CmaCh04G003300 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh04G003300
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPlant invertase/pectin methylesterase inhibitor
LocationCma_Chr04 : 1628739 .. 1629326 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAATCCGCCTTCCTTCTCCTACTCCTCTCTATCTCCACCCTATTCGCCACCACCAGCGCTGCCGGCTATGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACATACCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAGCCCTCGCCAATTAGTCCAAACAGCTCTAACGGTGAGCCTCTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGGAGATCGAAGAGGAGAGATTTCGAGTGGCACATTAGCAACGTGGAGACCTGGGTGAGCGCGGCGATGACGGACGGTAACACTTGCTCCGATGGATTTGCAGGGGCGGTGTTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATGCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG

mRNA sequence

ATGGCGAAATCCGCCTTCCTTCTCCTACTCCTCTCTATCTCCACCCTATTCGCCACCACCAGCGCTGCCGGCTATGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACATACCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAGCCCTCGCCAATTAGTCCAAACAGCTCTAACGGTGAGCCTCTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGGAGATCGAAGAGGAGAGATTTCGAGTGGCACATTAGCAACGTGGAGACCTGGGTGAGCGCGGCGATGACGGACGGTAACACTTGCTCCGATGGATTTGCAGGGGCGGTGTTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATGCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG

Coding sequence (CDS)

ATGGCGAAATCCGCCTTCCTTCTCCTACTCCTCTCTATCTCCACCCTATTCGCCACCACCAGCGCTGCCGGCTATGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACATACCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAGCCCTCGCCAATTAGTCCAAACAGCTCTAACGGTGAGCCTCTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGGAGATCGAAGAGGAGAGATTTCGAGTGGCACATTAGCAACGTGGAGACCTGGGTGAGCGCGGCGATGACGGACGGTAACACTTGCTCCGATGGATTTGCAGGGGCGGTGTTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATGCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG

Protein sequence

MAKSAFLLLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQSPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALALINNYAQNQS
Homology
[Close]
BLAST of CmaCh04G003300 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.9e-45
Identity = 91/181 (50.28%), Postives = 129/181 (71.27%), Query Frame = 1 

Query: 11  LSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQSPRQLVQTALTV 70
           L  S L    +AA    A+ FIK+ C  TTYP +C QSL+ +A  IQ +P++L  TAL V
Sbjct: 8   LVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQV 67

Query: 71  SLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRRD 130
           SL+  + A++F+ +L KF GLK R+YAA+ DCLEEV D+++R+S+S +E+K +  +K  D
Sbjct: 68  SLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGND 127

Query: 131 FEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALALINNY 190
           F + +SNVETWVSAA+TD  TC DGFAG  ++G+IK S+R ++V + R  SNALAL+NN+
Sbjct: 128 FTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNF 187

Query: 191 A 192
           A
Sbjct: 188 A 188

[Close]
BLAST of CmaCh04G003300 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.4e-16
Identity = 59/197 (29.95%), Postives = 104/197 (52.79%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATTSAAGYGGASN------FIKSRCNFTTYPDLCVQSLTNF-- 60
           +A  A LL++ ++  + A  ++    G +        +KS C+ T YPDLC  ++     
Sbjct: 32  LALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAVPE 91

Query: 61  ASRIQQSPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVER 120
           AS+   S + +++ +L ++ +        + KL K T L  RE  AL DCLE + +T++ 
Sbjct: 92  ASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDETLDE 151

Query: 121 LSKSVEELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRR 180
           L K+VE+L+      ++    H  +++T +SAAMT+  TC DGF+    N  ++ ++   
Sbjct: 152 LHKAVEDLE--EYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSDG 211

Query: 181 IVDLTRFISNALALINN 190
            V + +  SNALA+I N
Sbjct: 212 QVHVEKMCSNALAMIKN 226

[Close]
BLAST of CmaCh04G003300 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.4e-16
Identity = 59/197 (29.95%), Postives = 104/197 (52.79%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATTSAAGYGGASN------FIKSRCNFTTYPDLCVQSLTNF-- 60
           +A  A LL++ ++  + A  ++    G +        +KS C+ T YPDLC  ++     
Sbjct: 32  LALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAVPE 91

Query: 61  ASRIQQSPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVER 120
           AS+   S + +++ +L ++ +        + KL K T L  RE  AL DCLE + +T++ 
Sbjct: 92  ASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDETLDE 151

Query: 121 LSKSVEELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRR 180
           L K+VE+L+      ++    H  +++T +SAAMT+  TC DGF+    N  ++ ++   
Sbjct: 152 LHKAVEDLE--EYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSDG 211

Query: 181 IVDLTRFISNALALINN 190
            V + +  SNALA+I N
Sbjct: 212 QVHVEKMCSNALAMIKN 226

[Close]
BLAST of CmaCh04G003300 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 7.0e-16
Identity = 58/195 (29.74%), Postives = 103/195 (52.82%), Query Frame = 1 

Query: 7   LLLLLSISTLFATTSAAGYGG--------ASNFIKSRCNFTTYPDLCVQSLTNFASRIQQ 66
           LLLL SI  + ATT+              +   +KS C+ T YP+LC  ++     +   
Sbjct: 29  LLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELT 88

Query: 67  SPRQLVQTALTVSLSHARSARSFVWKL-TKFTGLKPREYAALRDCLEEVGDTVERLSKSV 126
           S +++++ +L ++    +     V KL  K  GL PRE  AL DCLE + +T++ L  +V
Sbjct: 89  SQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAV 148

Query: 127 EELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLT 186
           E+L   +  K++    H  +++T +S+A+T+  TC DGF+    + +++ ++ +  V + 
Sbjct: 149 EDLH--QYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVE 208

Query: 187 RFISNALALINNYAQ 193
              SNALA+I N  +
Sbjct: 209 HMCSNALAMIKNMTE 221

[Close]
BLAST of CmaCh04G003300 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-15
Identity = 50/165 (30.30%), Postives = 95/165 (57.58%), Query Frame = 1 

Query: 28  ASNFIKSRCNFTTYPDLCVQSLTNFA--SRIQQSPRQLVQTALTVSLSHARSARSFVWKL 87
           A   +KS C+ T +P+LC  ++ N +  S+   S + +++ +L +++   R     V +L
Sbjct: 62  AHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKEL 121

Query: 88  TKFT-GLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRRDFEWHISNVETWVSA 147
            K   GL PRE  AL DCLE + +T++ L  +VE+L+L     ++  + H+ +++T +S+
Sbjct: 122 IKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELY--PNKKSLKEHVEDLKTLISS 181

Query: 148 AMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALALINN 190
           A+T+  TC DGF+    + +++  + +    + +  SNALA+I N
Sbjct: 182 AITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICN 224

[Close]
BLAST of CmaCh04G003300 vs. TrEMBL
Match: A0A0A0L8D7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176260 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 4.1e-71
Identity = 148/196 (75.51%), Postives = 172/196 (87.76%), Query Frame = 1 

Query: 1   MAKS-AFLLLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQS 60
           MAKS + LLLLLSI T+ A+   A  GGAS+FIKS+C+  TYPDLCVQSL++F+S IQ++
Sbjct: 1   MAKSLSLLLLLLSILTISAS---AFNGGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRN 60

Query: 61  PRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEE 120
           PRQLVQTAL VSLSHA+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRF 180
           LK V  SK +DF+WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR 
Sbjct: 121 LKRVSGSK-KDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALALINNYAQNQS 196
           ISNAL+LIN YA+NQS
Sbjct: 181 ISNALSLINKYAENQS 192

[Close]
BLAST of CmaCh04G003300 vs. TrEMBL
Match: B9R9Q7_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500010 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.7e-56
Identity = 118/193 (61.14%), Postives = 149/193 (77.20%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATT--SAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQ 60
           M K   LLLL+S+    ++T  SAA    ASNFIK+ C  TTYP LC+QSL+ +A+ IQQ
Sbjct: 1   MVKPTLLLLLVSVLLYISSTATSAAAPAAASNFIKASCRTTTYPALCIQSLSAYATSIQQ 60

Query: 61  SPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVE 120
           +P QL QTAL+VSL +A+S R+FV+KLTKF G+KPRE AAL+DCLEE+ DT +RLSKS  
Sbjct: 61  NPLQLTQTALSVSLENAQSTRTFVYKLTKFKGVKPREMAALKDCLEEIDDTADRLSKSYN 120

Query: 121 ELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTR 180
           ELK +   K +DF+WH+SNVETWVSAA+TD NTC+DGFAG  LNG++KSSI+ RIV + +
Sbjct: 121 ELKNLGHYKGKDFQWHMSNVETWVSAALTDENTCTDGFAGKALNGKMKSSIKARIVKVAQ 180

Query: 181 FISNALALINNYA 192
             SNAL+LIN YA
Sbjct: 181 VTSNALSLINKYA 193

[Close]
BLAST of CmaCh04G003300 vs. TrEMBL
Match: M5X6V6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011831mg PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 3.1e-55
Identity = 111/191 (58.12%), Postives = 150/191 (78.53%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQSP 60
           MA+++F LLLL I     T ++      +NFIKS C+ TTYP +C+QSL+++A+ IQQSP
Sbjct: 1   MARASFFLLLLPIFYFVGTANSTATTSDTNFIKSSCSATTYPQVCIQSLSSYATSIQQSP 60

Query: 61  RQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQL Q AL+VSL+ A+ A++FV KL KF GLK REY A++DCLEE+GDTV+RLSKSV+EL
Sbjct: 61  RQLAQAALSVSLTRAQKAKTFVTKLAKFKGLKAREYGAVKDCLEEMGDTVDRLSKSVQEL 120

Query: 121 KLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K + +SK +DF WH+SNVETWVSAA+TD NTC DGF+G  L+G+IK+SIR +++++ +  
Sbjct: 121 KNMGKSKGQDFVWHMSNVETWVSAALTDDNTCLDGFSGKALDGKIKASIRAQVLNVAQCT 180

Query: 181 SNALALINNYA 192
           SNALAL N +A
Sbjct: 181 SNALALCNRFA 191

[Close]
BLAST of CmaCh04G003300 vs. TrEMBL
Match: A9PDL3_POPTR (21K protein OS=Populus trichocarpa GN=POPTR_0012s14560g PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 4.1e-55
Identity = 117/194 (60.31%), Postives = 147/194 (75.77%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATTSAAGY----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +LLLLSI  +  T ++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQSPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            QSPRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD NTC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDENTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNALALINNY 191
            R  SNALALIN +
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of CmaCh04G003300 vs. TrEMBL
Match: A9PD34_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.0e-54
Identity = 116/194 (59.79%), Postives = 146/194 (75.26%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATTSAAGY----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +LLLLSI  +  T ++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQSPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            QSPRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD  TC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDEKTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNALALINNY 191
            R  SNALALIN +
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of CmaCh04G003300 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 188.3 bits (477), Expect = 4.3e-48
Identity = 102/200 (51.00%), Postives = 142/200 (71.00%), Query Frame = 1 

Query: 1   MAKSAF---LLLLLSISTLFATTSAAGYGGAS----NFIKSRCNFTTYPDLCVQSLTNFA 60
           MAK  F    L LLS + L A++SA      S    NFI++ C  TTYP +CV SLT +A
Sbjct: 1   MAKQIFYTLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYA 60

Query: 61  SRIQQSPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERL 120
           + IQ SPR+L +TAL V+++ A+S + FVW+L +FT LK RE  A++DC+EE+ D V+RL
Sbjct: 61  NSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRL 120

Query: 121 SKSVEELKLVRRSKRRDFEW-HISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRR 180
           + S+ E+K+   +K RD  W H+SN +TW SAA+T+ NTCSDGFAG V++GR+K+S+R R
Sbjct: 121 TMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRAR 180

Query: 181 IVDLTRFISNALALINNYAQ 193
           I++L R  SNALALIN +A+
Sbjct: 181 ILNLGRGTSNALALINAFAK 200

[Close]
BLAST of CmaCh04G003300 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 188.0 bits (476), Expect = 5.7e-48
Identity = 106/201 (52.74%), Postives = 147/201 (73.13%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFA----TTSAAGYGGAS----NFIKSRCNFTTYPDLCVQSLTNF 60
           MAK  +L +LLSIS L +      +AA   GAS    NFI+S C  TTYP LCV SL+ +
Sbjct: 1   MAKQ-YLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVY 60

Query: 61  ASRIQQSPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVER 120
           A+ IQ SP++L +TA+ V+LS A+S + FV +LT+  GLK RE  A++DC+EE+ DTV+R
Sbjct: 61  ANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDR 120

Query: 121 LSKSVEELKLVRRSKRRD-FEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRR 180
           L+KSV+ELKL   +K +D F +H+SN +TW SAA+TD NTCSDGF+G V++GRIK+S+R 
Sbjct: 121 LTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRA 180

Query: 181 RIVDLTRFISNALALINNYAQ 193
           RI+++    SNAL+LIN +A+
Sbjct: 181 RIMNVGHETSNALSLINAFAK 200

[Close]
BLAST of CmaCh04G003300 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 166.8 bits (421), Expect = 1.4e-41
Identity = 92/186 (49.46%), Postives = 124/186 (66.67%), Query Frame = 1 

Query: 8   LLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQSPRQLVQTA 67
           L LL  ST+   +S+     + NFI S C  T Y  LCV+ L  FA +I+++  QL QTA
Sbjct: 16  LPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTA 75

Query: 68  LTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRS- 127
           L V+L   +S   +V KLTK   +K REY A++DC+E +GD +E L++S+ ELK V RS 
Sbjct: 76  LAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSG 135

Query: 128 -KRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALA 187
             R +F W +SNVETWVSAA+TD  TC DGF G V++G +KS+IRRR+V + R  SNALA
Sbjct: 136 RDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALA 195

Query: 188 LINNYA 192
           L+N +A
Sbjct: 196 LVNRFA 201

[Close]
BLAST of CmaCh04G003300 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 163.3 bits (412), Expect = 1.5e-40
Identity = 86/187 (45.99%), Postives = 125/187 (66.84%), Query Frame = 1 

Query: 6   FLLLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQSPRQLVQ 65
           F+L L + + + A        G + FIK+ C  T+YPD C QSL+++AS I++ PR+L +
Sbjct: 12  FVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAE 71

Query: 66  TALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELK-LVR 125
           TAL VS++ A+SA+++V ++T + G+  R++ A+ DCLEE+GDTV+RLS S++ELK L  
Sbjct: 72  TALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEE 131

Query: 126 RSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNAL 185
                DF + +SNV TW SAA+TD   C DGF G  + G +KS IR  IV +    SNAL
Sbjct: 132 GDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNAL 191

Query: 186 ALINNYA 192
           ALIN++A
Sbjct: 192 ALINDFA 198

[Close]
BLAST of CmaCh04G003300 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 154.8 bits (390), Expect = 5.3e-38
Identity = 86/198 (43.43%), Postives = 132/198 (66.67%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQSP 60
           +  + FL++LLSI+ L ++++      +  FI S C  T YP LCV +L+ +A++I+ + 
Sbjct: 3   LKNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNN 62

Query: 61  RQ-LVQTALTVSLSHARSARSFVWKLTKFTG-LKPREYAALRDCLEEVGDTVERLSKSVE 120
            Q L QTAL +SL+ A+S   FV KLTK T   K REY A++DC+E +G++V+RL++SV+
Sbjct: 63  DQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVK 122

Query: 121 ELKLVRRS-KRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLT 180
           EL     +    DF W +SNV+TWVSAA+TD  TC DGF+   + G++K  IR ++V + 
Sbjct: 123 ELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVA 182

Query: 181 RFISNALALINNYAQNQS 196
           +  SNALAL+N +A+ +S
Sbjct: 183 QVTSNALALVNQFAEKRS 200

[Close]
BLAST of CmaCh04G003300 vs. NCBI nr
Match: gi|659077241|ref|XP_008439100.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 276.9 bits (707), Expect = 2.6e-71
Identity = 148/196 (75.51%), Postives = 170/196 (86.73%), Query Frame = 1 

Query: 1   MAKS-AFLLLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQS 60
           MAKS + LLLLLSI T+ A+   A  GGASNFIKS+C+  TYPDLCVQSL++FAS IQ++
Sbjct: 1   MAKSLSLLLLLLSILTISAS---AFNGGASNFIKSKCSAATYPDLCVQSLSSFASTIQRN 60

Query: 61  PRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEE 120
           PRQLVQTAL VSLS A+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRF 180
           LK V  SK++DF WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR 
Sbjct: 121 LKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALALINNYAQNQS 196
           ISNAL+LIN YA+ QS
Sbjct: 181 ISNALSLINKYAETQS 193

[Close]
BLAST of CmaCh04G003300 vs. NCBI nr
Match: gi|449460808|ref|XP_004148136.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 275.8 bits (704), Expect = 5.9e-71
Identity = 148/196 (75.51%), Postives = 172/196 (87.76%), Query Frame = 1 

Query: 1   MAKS-AFLLLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQS 60
           MAKS + LLLLLSI T+ A+   A  GGAS+FIKS+C+  TYPDLCVQSL++F+S IQ++
Sbjct: 1   MAKSLSLLLLLLSILTISAS---AFNGGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRN 60

Query: 61  PRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEE 120
           PRQLVQTAL VSLSHA+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRF 180
           LK V  SK +DF+WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR 
Sbjct: 121 LKRVSGSK-KDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALALINNYAQNQS 196
           ISNAL+LIN YA+NQS
Sbjct: 181 ISNALSLINKYAENQS 192

[Close]
BLAST of CmaCh04G003300 vs. NCBI nr
Match: gi|255539735|ref|XP_002510932.1| (PREDICTED: 21 kDa protein [Ricinus communis])

HSP 1 Score: 227.3 bits (578), Expect = 2.4e-56
Identity = 118/193 (61.14%), Postives = 149/193 (77.20%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATT--SAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQ 60
           M K   LLLL+S+    ++T  SAA    ASNFIK+ C  TTYP LC+QSL+ +A+ IQQ
Sbjct: 1   MVKPTLLLLLVSVLLYISSTATSAAAPAAASNFIKASCRTTTYPALCIQSLSAYATSIQQ 60

Query: 61  SPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVE 120
           +P QL QTAL+VSL +A+S R+FV+KLTKF G+KPRE AAL+DCLEE+ DT +RLSKS  
Sbjct: 61  NPLQLTQTALSVSLENAQSTRTFVYKLTKFKGVKPREMAALKDCLEEIDDTADRLSKSYN 120

Query: 121 ELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTR 180
           ELK +   K +DF+WH+SNVETWVSAA+TD NTC+DGFAG  LNG++KSSI+ RIV + +
Sbjct: 121 ELKNLGHYKGKDFQWHMSNVETWVSAALTDENTCTDGFAGKALNGKMKSSIKARIVKVAQ 180

Query: 181 FISNALALINNYA 192
             SNAL+LIN YA
Sbjct: 181 VTSNALSLINKYA 193

[Close]
BLAST of CmaCh04G003300 vs. NCBI nr
Match: gi|596049257|ref|XP_007220475.1| (hypothetical protein PRUPE_ppa011831mg [Prunus persica])

HSP 1 Score: 223.0 bits (567), Expect = 4.5e-55
Identity = 111/191 (58.12%), Postives = 150/191 (78.53%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQSP 60
           MA+++F LLLL I     T ++      +NFIKS C+ TTYP +C+QSL+++A+ IQQSP
Sbjct: 1   MARASFFLLLLPIFYFVGTANSTATTSDTNFIKSSCSATTYPQVCIQSLSSYATSIQQSP 60

Query: 61  RQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQL Q AL+VSL+ A+ A++FV KL KF GLK REY A++DCLEE+GDTV+RLSKSV+EL
Sbjct: 61  RQLAQAALSVSLTRAQKAKTFVTKLAKFKGLKAREYGAVKDCLEEMGDTVDRLSKSVQEL 120

Query: 121 KLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K + +SK +DF WH+SNVETWVSAA+TD NTC DGF+G  L+G+IK+SIR +++++ +  
Sbjct: 121 KNMGKSKGQDFVWHMSNVETWVSAALTDDNTCLDGFSGKALDGKIKASIRAQVLNVAQCT 180

Query: 181 SNALALINNYA 192
           SNALAL N +A
Sbjct: 181 SNALALCNRFA 191

[Close]
BLAST of CmaCh04G003300 vs. NCBI nr
Match: gi|224120250|ref|XP_002318283.1| (21K protein precursor [Populus trichocarpa])

HSP 1 Score: 222.6 bits (566), Expect = 5.9e-55
Identity = 117/194 (60.31%), Postives = 147/194 (75.77%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISTLFATTSAAGY----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +LLLLSI  +  T ++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQSPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            QSPRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRRDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD NTC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDENTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNALALINNY 191
            R  SNALALIN +
Sbjct: 181 ERVTSNALALINKF 194

The following BLAST results are available for this feature:
BLAST of CmaCh04G003300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA1.9e-4550.2821 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME1_CITSI1.4e-1629.95Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
PME3_CITSI1.4e-1629.95Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME2_ARATH7.0e-1629.74Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PMEU1_SOLLC1.2e-1530.30Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
BLAST of CmaCh04G003300 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8D7_CUCSA4.1e-7175.51Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176260 PE=4 SV=1[more]
B9R9Q7_RICCO1.7e-5661.1421 kDa protein, putative OS=Ricinus communis GN=RCOM_1500010 PE=4 SV=1[more]
M5X6V6_PRUPE3.1e-5558.12Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011831mg PE=4 SV=1[more]
A9PDL3_POPTR4.1e-5560.3121K protein OS=Populus trichocarpa GN=POPTR_0012s14560g PE=2 SV=1[more]
A9PD34_POPTR2.0e-5459.79Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1[more]
BLAST of CmaCh04G003300 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G47380.14.3e-4851.00 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.15.7e-4852.74 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.11.4e-4149.46 pectin methylesterase inhibitor 1[more]
AT4G25260.11.5e-4045.99 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62770.15.3e-3843.43 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of CmaCh04G003300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659077241|ref|XP_008439100.1|2.6e-7175.51PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|449460808|ref|XP_004148136.1|5.9e-7175.51PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|255539735|ref|XP_002510932.1|2.4e-5661.14PREDICTED: 21 kDa protein [Ricinus communis][more]
gi|596049257|ref|XP_007220475.1|4.5e-5558.12hypothetical protein PRUPE_ppa011831mg [Prunus persica][more]
gi|224120250|ref|XP_002318283.1|5.9e-5560.3121K protein precursor [Populus trichocarpa][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G003300.1CmaCh04G003300.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 29..189
score: 2.7
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..186
score: 2.3
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..186
score: 1.2
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 1..190
score: 5.1
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 29..190
score: 1.1
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 6..192
score: 1.5
NoneNo IPR availablePANTHERPTHR31080:SF9INVERTASE/PECTIN METHYLESTERASE INHIBITOR FAMILY PROTEIN / DC 1.2-LIKE PROTEIN-RELATEDcoord: 6..192
score: 1.5