Cp4.1LG14g05770 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG14g05770
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPlant invertase/pectin methylesterase inhibitor
LocationCp4.1LG14 : 848177 .. 848761 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACATTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCTTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTCCAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACATTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACTGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCGAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAGTTGACCAGTAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

mRNA sequence

ATGGGTGTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACATTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCTTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTCCAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACATTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACTGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCGAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAGTTGACCAGTAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

Coding sequence (CDS)

ATGGGTGTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACATTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCTTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTCCAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACATTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACTGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCGAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAGTTGACCAGTAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

Protein sequence

MGVSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAELH
Homology
[Close]
BLAST of Cp4.1LG14g05770 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 9.4e-37
Identity = 86/195 (44.10%), Postives = 132/195 (67.69%), Query Frame = 1 

Query: 3   VSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTS 62
           + + K +L+F  +++ L    +A +   + QFI+TSCT T YP +C  SLS +A +IQ +
Sbjct: 1   MKLSKSTLVFSALLVIL----AAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNN 60

Query: 63  PRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISE 122
           P+ LA+TAL V+L+  + A T + +L+   GL  R  +A++DCLEE+ DS+D ++ S  E
Sbjct: 61  PQELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDE 120

Query: 123 MPKL---RGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQL 182
           M  L   +G+D    MS+V+TWVSAALTDETTC +GF GK ++G +K +VR ++V +A++
Sbjct: 121 MKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARV 180

Query: 183 TSNALSLINRIAELH 195
           TSNAL+L+N  A  H
Sbjct: 181 TSNALALVNNFAAKH 191

[Close]
BLAST of Cp4.1LG14g05770 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 2.7e-15
Identity = 58/176 (32.95%), Postives = 102/176 (57.95%), Query Frame = 1 

Query: 26  ITPQSSTQ--FIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 85
           IT  SST    +++ C+ST YP LCFS+++       TS + +   +L++T  +VK    
Sbjct: 51  ITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYF 110

Query: 86  QILKL-SHAHGLPPRDVSALNDCLEELSDSVDSLASSISEM---PK---LRGHDIDLVMS 145
            + KL +   GL PR+V+AL+DCLE + +++D L  ++ ++   PK   LR H       
Sbjct: 111 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKH-----AD 170

Query: 146 DVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           D++T +S+A+T++ TC +GF     +  V+ A+ +  V++  + SNAL++I  + E
Sbjct: 171 DLKTLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTE 221

[Close]
BLAST of Cp4.1LG14g05770 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 8.5e-14
Identity = 49/170 (28.82%), Postives = 97/170 (57.06%), Query Frame = 1 

Query: 30  SSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKL- 89
           SS   +R+SC+ST+YP LC S++        TS + +   ++++T+++V+     + KL 
Sbjct: 54  SSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLI 113

Query: 90  SHAHGLPPRDVSALNDCLEELSDSVDSLASSISEM------PKLRGHDIDLVMSDVQTWV 149
               GL PR+ +AL+DCLE + +++D L  ++ ++        LR H       D++T +
Sbjct: 114 KKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREH-----AGDLKTLI 173

Query: 150 SAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           S+A+T++ TC +GF     +  V+ A+ +  +++  + SNAL++I  + +
Sbjct: 174 SSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTD 218

[Close]
BLAST of Cp4.1LG14g05770 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 7.2e-13
Identity = 62/200 (31.00%), Postives = 98/200 (49.00%), Query Frame = 1 

Query: 7   KKSLIFVQIILQLTFIHSAIT-----------------PQSSTQFIRTSCTSTKYPRLCF 66
           KK L+   I+L ++ I +A                    +  +Q I  +C  T++P LC 
Sbjct: 42  KKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCV 101

Query: 67  SSLSIHANSIQ-TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEE 126
            SL     S+  +S + L    +++TL     A      LS    +PPR  SA + C+E 
Sbjct: 102 DSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVD-MPPRARSAYDSCVEL 161

Query: 127 LSDSVDSLASSISEMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAA 186
           L DSVD+L+ ++S +             DV TW+SAALT+  TC+EGF G   +GGVK  
Sbjct: 162 LDDSVDALSRALSSVVSSSAKP-----QDVTTWLSAALTNHDTCTEGFDG-VDDGGVKDH 221

Query: 187 VRRRIVNIAQLTSNALSLIN 189
           +   + N+++L SN L++ +
Sbjct: 222 MTAALQNLSELVSNCLAIFS 234

[Close]
BLAST of Cp4.1LG14g05770 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 51/156 (32.69%), Postives = 83/156 (53.21%), Query Frame = 1 

Query: 32  TQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHA 91
           TQ I  +C+ + YP LC  +L     S+      L   + + TL     A      +++ 
Sbjct: 72  TQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYT 131

Query: 92  HGLPPRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDIDLVMSDVQTWVSAALTDETT 151
             +PPR  SA + CLE L DSVD+L  ++S +  + G   D   SDV TW+S+A+T+  T
Sbjct: 132 Q-MPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSG---DESHSDVMTWLSSAMTNHDT 191

Query: 152 CSEGFQGKAVEGG-VKAAVRRRIVNIAQLTSNALSL 187
           C++GF     +GG VK  V   + +++++ SN L++
Sbjct: 192 CTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAI 223

[Close]
BLAST of Cp4.1LG14g05770 vs. TrEMBL
Match: A0A0A0LB27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 1.8e-79
Identity = 161/194 (82.99%), Postives = 178/194 (91.75%), Query Frame = 1 

Query: 1   MGVSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MG+S  KK+LI  Q+ILQLT IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP RDVSAL+DCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLT 180
           SEMPKLRG + DL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIA+LH
Sbjct: 181 SNALSLINRIADLH 194

[Close]
BLAST of Cp4.1LG14g05770 vs. TrEMBL
Match: A0A0A0L8D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 6.9e-79
Identity = 160/193 (82.90%), Postives = 176/193 (91.19%), Query Frame = 1 

Query: 2   GVSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQT 61
           G+S  KKSLI  Q+ILQLT IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSIS 121
           SPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP RDVSAL+DCLEELSDSVDSLA+SIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTS 181
           EMPKLRG + DL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIAELH 195
           NALSLINRI +LH
Sbjct: 224 NALSLINRIGDLH 236

[Close]
BLAST of Cp4.1LG14g05770 vs. TrEMBL
Match: M5XKR0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.6e-51
Identity = 109/179 (60.89%), Postives = 140/179 (78.21%), Query Frame = 1 

Query: 16  ILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTL 75
           I+  T     + P+++T+FI+TSC++T YP+LC +SLS HA++IQTSP+L+A  AL+VTL
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDIDLVM 135
           SS KS +  +LKLS +HGL P++V A+ DCLEELSDSVD L SSI+EM   + +D  L++
Sbjct: 84  SSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           SDVQTWVSAALTDE TCSEGF G  + G +K AVR RIVNIAQLTSNAL+LINR A +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]
BLAST of Cp4.1LG14g05770 vs. TrEMBL
Match: B9ICT6_POPTR (Ripening-related family protein OS=Populus trichocarpa GN=POPTR_0015s14690g PE=4 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-49
Identity = 108/190 (56.84%), Postives = 146/190 (76.84%), Query Frame = 1 

Query: 5   ICKKSLIFVQIILQLTFIHSA-ITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSP 64
           +  KSLI + I   +    +A +TPQSS +FIRTSC++T YPRLC++SLSIH+ +I TSP
Sbjct: 4   LTSKSLILIAISFFINSSSAATVTPQSSIEFIRTSCSTTTYPRLCYTSLSIHSRTIHTSP 63

Query: 65  RLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISEM 124
           +L+A  AL+VTLSS KS +T + KLS +HGL P++VSA+ DC+EELSD+V  L  SI EM
Sbjct: 64  KLIANAALNVTLSSAKSTSTMMSKLSQSHGLKPKEVSAMKDCVEELSDAVYELRESIDEM 123

Query: 125 PKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNA 184
             ++  D ++++SDV+TWVSAA+TDE+TCS+GF G A+ G +K AVR RI+NIAQLTSNA
Sbjct: 124 DHVKRSDFEVMISDVRTWVSAAMTDESTCSDGFAGNAMNGNLKRAVRGRIMNIAQLTSNA 183

Query: 185 LSLINRIAEL 194
           L+L+N  A L
Sbjct: 184 LALVNNYALL 193

[Close]
BLAST of Cp4.1LG14g05770 vs. TrEMBL
Match: A0A061G793_THECC (DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 3.3e-49
Identity = 109/197 (55.33%), Postives = 147/197 (74.62%), Query Frame = 1 

Query: 4   SICKKSLIFVQIILQL-TFIHSA-----ITPQSSTQFIRTSCTSTKYPRLCFSSLSIHAN 63
           + C  S  F+ I+L + ++I+ +     I PQSS +FIRTSC+ST YP+LCF +LS HA+
Sbjct: 4   TFCSHSFKFLLILLAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLSTHAS 63

Query: 64  SIQTSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLA 123
            IQTSP+LLA  AL+VTLS+ +S +  ++ LS +HGL PR+  A+ DC+EELSDS+D L 
Sbjct: 64  LIQTSPQLLAHAALNVTLSTTESTSAMMVTLSKSHGLKPREAEAMQDCVEELSDSIDELR 123

Query: 124 SSISEMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIA 183
            SISEM +++G +  L+++DVQTWVSAALTDE+TCS+GFQG  + G VK AVR +I+ IA
Sbjct: 124 KSISEMGQVKGSNFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTKILRIA 183

Query: 184 QLTSNALSLINRIAELH 195
            LTSNAL+LIN  A LH
Sbjct: 184 HLTSNALALINNYASLH 200

[Close]
BLAST of Cp4.1LG14g05770 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 176.4 bits (446), Expect = 1.7e-44
Identity = 94/187 (50.27%), Postives = 137/187 (73.26%), Query Frame = 1 

Query: 12  FVQIILQLTFIHSAITPQSST----QFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLA 71
           F+ II  L  +H+  T  ++T    +F+++SCT T YPRLCFSSLS HA+ IQTSP+L+A
Sbjct: 17  FLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMA 76

Query: 72  TTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISEMPKLR 131
             AL++TL+S K  +  +++LS++  L P++VSA+ DC+EEL D+++ L  SI EM +L 
Sbjct: 77  HAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLS 136

Query: 132 GHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLI 191
           G + ++ +SD+QTWVSAALTD  TC++GF+G+ ++G VK  VR RI+ IA LTSNAL+LI
Sbjct: 137 GSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALI 196

Query: 192 NRIAELH 195
           N  A +H
Sbjct: 197 NHFASIH 202

[Close]
BLAST of Cp4.1LG14g05770 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-36
Identity = 83/171 (48.54%), Postives = 116/171 (67.84%), Query Frame = 1 

Query: 29  QSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKL 88
           + +  FI++SC +T YP LC  SLS++AN IQTSP+ LA TA++VTLS  +S    + +L
Sbjct: 33  KKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRL 92

Query: 89  SHAHGLPPRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDID-----LVMSDVQTWVS 148
           +   GL  R+V A+ DC+EE++D+VD L  S+ E+ KL G   D       MS+ QTW S
Sbjct: 93  TRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQEL-KLCGSAKDQDQFAYHMSNAQTWTS 152

Query: 149 AALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           AALTDE TCS+GF G+ ++G +K +VR RI+N+   TSNALSLIN  A+ +
Sbjct: 153 AALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFAKTY 202

[Close]
BLAST of Cp4.1LG14g05770 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 144.4 bits (363), Expect = 7.1e-35
Identity = 78/167 (46.71%), Postives = 112/167 (67.07%), Query Frame = 1 

Query: 32  TQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHA 91
           T+FI+ SC +T YP  CF SLS +A+ I+  PR LA TAL+V+++  KSA T + +++  
Sbjct: 35  TEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDY 94

Query: 92  HGLPPRDVSALNDCLEELSDSVDSLASSISEMPKLR----GHDIDLVMSDVQTWVSAALT 151
            G+  R   A+ DCLEE+ D+VD L++S+ E+  L     G D    +S+V+TW SAALT
Sbjct: 95  KGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALT 154

Query: 152 DETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           DET C +GF GKA+ G +K+ +R  IV++A+ TSNAL+LIN  A  H
Sbjct: 155 DETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]
BLAST of Cp4.1LG14g05770 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 141.0 bits (354), Expect = 7.9e-34
Identity = 85/197 (43.15%), Postives = 126/197 (63.96%), Query Frame = 1 

Query: 5   ICKKSLIFVQIILQLTFIH--SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTS 64
           +C   L+F+ ++ Q T     S+  P SS  FI +SC  T+Y  LC   L+  A+ I+ +
Sbjct: 8   LCYCLLLFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRN 67

Query: 65  PRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISE 124
              LA TAL+VTL  V+S    + KL+ A  +  R+  A+ DC+E L D ++ LA S+ E
Sbjct: 68  ENQLAQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRE 127

Query: 125 MPKL--RGHDIDLVM---SDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIA 184
           + ++   G D D  +   S+V+TWVSAALTDETTC +GF GK ++G VK+A+RRR+V++A
Sbjct: 128 LKQVGRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVA 187

Query: 185 QLTSNALSLINRIAELH 195
           ++TSNAL+L+NR A  H
Sbjct: 188 RVTSNALALVNRFAARH 204

[Close]
BLAST of Cp4.1LG14g05770 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 140.2 bits (352), Expect = 1.3e-33
Identity = 80/173 (46.24%), Postives = 115/173 (66.47%), Query Frame = 1 

Query: 24  SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 83
           +AIT + +  FI+ SC +T YP +C +SL+ +ANSIQTSPR LA TAL+VT++  +S   
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 84  QILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISEMP---KLRGHD-IDLVMSDVQ 143
            + +L     L  R++ A+ DC+EE+ D+VD L  SI E+      +G D     MS+ Q
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQ 147

Query: 144 TWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           TW SAALT+  TCS+GF G+ ++G VK +VR RI+N+ + TSNAL+LIN  A+
Sbjct: 148 TWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAK 200

[Close]
BLAST of Cp4.1LG14g05770 vs. NCBI nr
Match: gi|778679325|ref|XP_004148138.2| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 303.5 bits (776), Expect = 2.6e-79
Identity = 161/194 (82.99%), Postives = 178/194 (91.75%), Query Frame = 1 

Query: 1   MGVSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MG+S  KK+LI  Q+ILQLT IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP RDVSAL+DCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLT 180
           SEMPKLRG + DL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIA+LH
Sbjct: 181 SNALSLINRIADLH 194

[Close]
BLAST of Cp4.1LG14g05770 vs. NCBI nr
Match: gi|778688522|ref|XP_011652768.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 301.6 bits (771), Expect = 9.9e-79
Identity = 160/193 (82.90%), Postives = 176/193 (91.19%), Query Frame = 1 

Query: 2   GVSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQT 61
           G+S  KKSLI  Q+ILQLT IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSIS 121
           SPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP RDVSAL+DCLEELSDSVDSLA+SIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTS 181
           EMPKLRG + DL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIAELH 195
           NALSLINRI +LH
Sbjct: 224 NALSLINRIGDLH 236

[Close]
BLAST of Cp4.1LG14g05770 vs. NCBI nr
Match: gi|596006342|ref|XP_007218426.1| (hypothetical protein PRUPE_ppa011607mg [Prunus persica])

HSP 1 Score: 210.7 bits (535), Expect = 2.3e-51
Identity = 109/179 (60.89%), Postives = 140/179 (78.21%), Query Frame = 1 

Query: 16  ILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTL 75
           I+  T     + P+++T+FI+TSC++T YP+LC +SLS HA++IQTSP+L+A  AL+VTL
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDIDLVM 135
           SS KS +  +LKLS +HGL P++V A+ DCLEELSDSVD L SSI+EM   + +D  L++
Sbjct: 84  SSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           SDVQTWVSAALTDE TCSEGF G  + G +K AVR RIVNIAQLTSNAL+LINR A +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]
BLAST of Cp4.1LG14g05770 vs. NCBI nr
Match: gi|645257371|ref|XP_008234384.1| (PREDICTED: 21 kDa protein [Prunus mume])

HSP 1 Score: 209.1 bits (531), Expect = 6.7e-51
Identity = 109/179 (60.89%), Postives = 139/179 (77.65%), Query Frame = 1 

Query: 16  ILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTL 75
           I+  T     + P+++T+FI+TSC +T YP+LC +SLS HA++IQTSP+L+A  AL+VTL
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCRATTYPKLCRTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDIDLVM 135
           SS KS +  +LKLS +HGL P++V A+ DCLEELSDSVD L SSI+EM   + +D  L++
Sbjct: 84  SSAKSTSATMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           SDVQTWVSAALTDE TCSEGF G  + G +K AVR RIVNIAQLTSNAL+LINR A +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]
BLAST of Cp4.1LG14g05770 vs. NCBI nr
Match: gi|566207616|ref|XP_002322404.2| (ripening-related family protein [Populus trichocarpa])

HSP 1 Score: 203.8 bits (517), Expect = 2.8e-49
Identity = 108/190 (56.84%), Postives = 146/190 (76.84%), Query Frame = 1 

Query: 5   ICKKSLIFVQIILQLTFIHSA-ITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSP 64
           +  KSLI + I   +    +A +TPQSS +FIRTSC++T YPRLC++SLSIH+ +I TSP
Sbjct: 4   LTSKSLILIAISFFINSSSAATVTPQSSIEFIRTSCSTTTYPRLCYTSLSIHSRTIHTSP 63

Query: 65  RLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSISEM 124
           +L+A  AL+VTLSS KS +T + KLS +HGL P++VSA+ DC+EELSD+V  L  SI EM
Sbjct: 64  KLIANAALNVTLSSAKSTSTMMSKLSQSHGLKPKEVSAMKDCVEELSDAVYELRESIDEM 123

Query: 125 PKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLTSNA 184
             ++  D ++++SDV+TWVSAA+TDE+TCS+GF G A+ G +K AVR RI+NIAQLTSNA
Sbjct: 124 DHVKRSDFEVMISDVRTWVSAAMTDESTCSDGFAGNAMNGNLKRAVRGRIMNIAQLTSNA 183

Query: 185 LSLINRIAEL 194
           L+L+N  A L
Sbjct: 184 LALVNNYALL 193

The following BLAST results are available for this feature:
BLAST of Cp4.1LG14g05770 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA9.4e-3744.1021 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME2_ARATH2.7e-1532.95Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PME3_ARATH8.5e-1428.82Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
PME34_ARATH7.2e-1331.00Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P... [more]
PME61_ARATH1.6e-1232.69Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cp4.1LG14g05770 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LB27_CUCSA1.8e-7982.99Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1[more]
A0A0A0L8D1_CUCSA6.9e-7982.90Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1[more]
M5XKR0_PRUPE1.6e-5160.89Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1[more]
B9ICT6_POPTR2.0e-4956.84Ripening-related family protein OS=Populus trichocarpa GN=POPTR_0015s14690g PE=4... [more]
A0A061G793_THECC3.3e-4955.33DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1[more]
BLAST of Cp4.1LG14g05770 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G62360.11.7e-4450.27 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.11.3e-3648.54 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25260.17.1e-3546.71 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.17.9e-3443.15 pectin methylesterase inhibitor 1[more]
AT3G47380.11.3e-3346.24 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Cp4.1LG14g05770 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778679325|ref|XP_004148138.2|2.6e-7982.99PREDICTED: 21 kDa protein [Cucumis sativus][more]
gi|778688522|ref|XP_011652768.1|9.9e-7982.90PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|596006342|ref|XP_007218426.1|2.3e-5160.89hypothetical protein PRUPE_ppa011607mg [Prunus persica][more]
gi|645257371|ref|XP_008234384.1|6.7e-5160.89PREDICTED: 21 kDa protein [Prunus mume][more]
gi|566207616|ref|XP_002322404.2|2.8e-4956.84ripening-related family protein [Populus trichocarpa][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g05770.1Cp4.1LG14g05770.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 31..190
score: 1.7
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 34..186
score: 3.3
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 30..186
score: 8.8
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 7..189
score: 1.6
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 33..190
score: 7.19
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 10..194
score: 4.9
NoneNo IPR availablePANTHERPTHR31080:SF19PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEINcoord: 10..194
score: 4.9

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG14g05770CmaCh04G003320Cucurbita maxima (Rimu)cmacpeB715
Cp4.1LG14g05770MELO3C006819Melon (DHL92) v3.5.1cpemeB190
Cp4.1LG14g05770Lsi05G013170Bottle gourd (USVL1VR-Ls)cpelsiB172
Cp4.1LG14g05770MELO3C006819.2Melon (DHL92) v3.6.1cpemedB233
Cp4.1LG14g05770CsaV3_3G016210Cucumber (Chinese Long) v3cpecucB0257
Cp4.1LG14g05770Bhi01G000900Wax gourdcpewgoB0298
Cp4.1LG14g05770CsGy3G016070Cucumber (Gy14) v2cgybcpeB327
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG14g05770Cp4.1LG01g03820Cucurbita pepo (Zucchini)cpecpeB234
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG14g05770Cucurbita pepo (Zucchini)cpecpeB245
Cp4.1LG14g05770Cucumber (Gy14) v1cgycpeB0673
Cp4.1LG14g05770Cucurbita maxima (Rimu)cmacpeB344
Cp4.1LG14g05770Cucurbita moschata (Rifu)cmocpeB307
Cp4.1LG14g05770Cucurbita moschata (Rifu)cmocpeB668
Cp4.1LG14g05770Wild cucumber (PI 183967)cpecpiB217
Cp4.1LG14g05770Cucumber (Chinese Long) v2cpecuB214
Cp4.1LG14g05770Watermelon (Charleston Gray)cpewcgB207
Cp4.1LG14g05770Watermelon (97103) v1cpewmB227
Cp4.1LG14g05770Silver-seed gourdcarcpeB0523
Cp4.1LG14g05770Silver-seed gourdcarcpeB0998
Cp4.1LG14g05770Silver-seed gourdcarcpeB1215