CmaCh04G003320 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh04G003320
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cma_Chr04 : 1634013 .. 1634621 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTTTCGATTTGCAAGCAAAGCTTGATTCTTTTGGTAGCAATCTTCCAATTAACACTAAACCCAATCTTCATCCATTCAGCCATCACTCCACAAAGCAGCAGCAGCAGCAGCACTGAGTTCATTAAAACCTCATGCAGCTCCACCACTTACCCTCGTCTCTGTTTCTCATCCCTCTCACTCCACGCCACTTCCATCCAAACAAGCCCTCGCCTTCTGGCCACCGCCGCCCTCTCTGTCTCCATCTCCTCCGTCAAGTCTGCCACTACCCAAATTCTCAAGCTCTCACACGCTCACGGCCTCTCTCCCCGAGATGTCTCTGCTCTTAATGATTGTTTGGAAGAGCTGGGTGACTCTATCGACTCCCTCATCAGCTCCATTTCTGAGATGCCAAAGCTTCGAGGCAACGATTTTGATGTCGTTATGAGCAATATCCAGACGTGGGTCAGCGCTGCCCTCACCGATGAGACCACTTGTAGCGAGGGGTTTCAAGGGAAGACGGTGAACGGCGGCATTAAGGCAGCGGTGAGGTCGAAGATTGTGAACATTGCTCAGTTGACTAGTAATGCTATTTCTTTGATCAACCGGATTGCTGAGCTGCATTAG

mRNA sequence

ATGGGTTTTTCGATTTGCAAGCAAAGCTTGATTCTTTTGGTAGCAATCTTCCAATTAACACTAAACCCAATCTTCATCCATTCAGCCATCACTCCACAAAGCAGCAGCAGCAGCAGCACTGAGTTCATTAAAACCTCATGCAGCTCCACCACTTACCCTCGTCTCTGTTTCTCATCCCTCTCACTCCACGCCACTTCCATCCAAACAAGCCCTCGCCTTCTGGCCACCGCCGCCCTCTCTGTCTCCATCTCCTCCGTCAAGTCTGCCACTACCCAAATTCTCAAGCTCTCACACGCTCACGGCCTCTCTCCCCGAGATGTCTCTGCTCTTAATGATTGTTTGGAAGAGCTGGGTGACTCTATCGACTCCCTCATCAGCTCCATTTCTGAGATGCCAAAGCTTCGAGGCAACGATTTTGATGTCGTTATGAGCAATATCCAGACGTGGGTCAGCGCTGCCCTCACCGATGAGACCACTTGTAGCGAGGGGTTTCAAGGGAAGACGGTGAACGGCGGCATTAAGGCAGCGGTGAGGTCGAAGATTGTGAACATTGCTCAGTTGACTAGTAATGCTATTTCTTTGATCAACCGGATTGCTGAGCTGCATTAG

Coding sequence (CDS)

ATGGGTTTTTCGATTTGCAAGCAAAGCTTGATTCTTTTGGTAGCAATCTTCCAATTAACACTAAACCCAATCTTCATCCATTCAGCCATCACTCCACAAAGCAGCAGCAGCAGCAGCACTGAGTTCATTAAAACCTCATGCAGCTCCACCACTTACCCTCGTCTCTGTTTCTCATCCCTCTCACTCCACGCCACTTCCATCCAAACAAGCCCTCGCCTTCTGGCCACCGCCGCCCTCTCTGTCTCCATCTCCTCCGTCAAGTCTGCCACTACCCAAATTCTCAAGCTCTCACACGCTCACGGCCTCTCTCCCCGAGATGTCTCTGCTCTTAATGATTGTTTGGAAGAGCTGGGTGACTCTATCGACTCCCTCATCAGCTCCATTTCTGAGATGCCAAAGCTTCGAGGCAACGATTTTGATGTCGTTATGAGCAATATCCAGACGTGGGTCAGCGCTGCCCTCACCGATGAGACCACTTGTAGCGAGGGGTTTCAAGGGAAGACGGTGAACGGCGGCATTAAGGCAGCGGTGAGGTCGAAGATTGTGAACATTGCTCAGTTGACTAGTAATGCTATTTCTTTGATCAACCGGATTGCTGAGCTGCATTAG

Protein sequence

MGFSICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISLINRIAELH

Homology

[Close]

BLAST of CmaCh04G003320 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.2e-36
Identity = 84/176 (47.73%), Postives = 124/176 (70.45%), Query Frame = 1


Query: 30  ITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAALSVSISSVKSA 89
           I   +S++ + +FIKTSC+ TTYP +C  SLS +A +IQ +P+ LA+ AL VS++  + A
Sbjct: 16  ILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQA 75

Query: 90  TTQILKLSHAHGLSPRDVSALNDCLEELGDSIDSLISSISEMPKL---RGNDFDVVMSNI 149
            T + +L+   GL  R  +A++DCLEE+ DS+D +  S  EM  L   +GNDF   MSN+
Sbjct: 76  QTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNV 135

Query: 150 QTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISLINRIAELH 203
           +TWVSAALTDETTC +GF GK ++G IK +VR+++V +A++TSNA++L+N  A  H
Sbjct: 136 ETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNFAAKH 191

[Close]

BLAST of CmaCh04G003320 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 6.8e-14
Identity = 52/169 (30.77%), Postives = 99/169 (58.58%), Query Frame = 1


Query: 36  SSSSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAALSVSISSVKSATTQILK 95
           SS+S   +K+ CSST YP LCFS+++       TS + +  A+L+++  +VK     + K
Sbjct: 55  SSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKK 114

Query: 96  L-SHAHGLSPRDVSALNDCLEELGDSIDSL---ISSISEMPKLRGNDFDVVMSNIQTWVS 155
           L +   GL+PR+V+AL+DCLE + +++D L   +  + + PK +         +++T +S
Sbjct: 115 LIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQK--SLRKHADDLKTLIS 174

Query: 156 AALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISLINRIAE 201
           +A+T++ TC +GF     +  ++ A+    V++  + SNA+++I  + E
Sbjct: 175 SAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTE 221

[Close]

BLAST of CmaCh04G003320 vs. Swiss-Prot
Match: PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.4e-11
Identity = 64/204 (31.37%), Postives = 99/204 (48.53%), Query Frame = 1


Query: 9   SLILLVAIFQLTLNPIFIH---SAITPQSSSSSSTEF-----IKTSCSSTTYPRLCFSSL 68
           S I+L+A+    +  + IH   S  +P S S   TE      +K  C +T YP  CFSS+
Sbjct: 35  SSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSI 94

Query: 69  SLHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDS 128
           S    S  T P LL   +L V+I  + S  +   KL        R   A++ C    GD+
Sbjct: 95  SSLPESNTTDPELLFKLSLRVAIDELSSFPS---KLRANAEQDARLQKAIDVCSSVFGDA 154

Query: 129 IDSLISSISEMPKLRGN-DFDVVMSNIQTWVSAALTDETTCSE--GFQGKTVNGGIKAAV 188
           +D L  SIS +  + G       +SN++TW+SAALTD+ TC +  G    T   G    +
Sbjct: 155 LDRLNDSISALGTVAGRIASSASVSNVETWLSAALTDQDTCLDAVGELNSTAARGALQEI 214

Query: 189 RSKIVNIAQLTSNAISLINRIAEL 202
            + + N  +  SN+++++ +I  L
Sbjct: 215 ETAMRNSTEFASNSLAIVTKILGL 235

[Close]

BLAST of CmaCh04G003320 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 5.4e-11
Identity = 48/172 (27.91%), Postives = 98/172 (56.98%), Query Frame = 1


Query: 36  SSSSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAALSVSISSVKSATTQILK 95
           S SS   +++SCSST YP LC S++        TS + +  A+++++I++V+     + K
Sbjct: 52  SPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKK 111

Query: 96  L-SHAHGLSPRDVSALNDCLEELGDSIDSLISSISEM------PKLRGNDFDVVMSNIQT 155
           L     GL+PR+ +AL+DCLE + +++D L  ++ ++        LR +  D     ++T
Sbjct: 112 LIKKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGD-----LKT 171

Query: 156 WVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISLINRIAE 201
            +S+A+T++ TC +GF     +  ++ A+    +++  + SNA+++I  + +
Sbjct: 172 LISSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTD 218

[Close]

BLAST of CmaCh04G003320 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.7e-10
Identity = 54/208 (25.96%), Postives = 110/208 (52.88%), Query Frame = 1


Query: 4   SICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTE--------FIKTSCSSTTYPRL 63
           +I K+   L +A+F   L    +   +   +S  +S +         +K+SCSST YP L
Sbjct: 22  NIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDL 81

Query: 64  CFSSLSL--HATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDC 123
           CFS+++    A+   TS + +   +L+++ ++V+     I KL     L+ R+  AL+DC
Sbjct: 82  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDC 141

Query: 124 LEELGDSIDSLISSISEMPKLRGN-DFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGG 183
           LE + +++D L  ++ ++ +            +++T +SAA+T++ TC +GF     N  
Sbjct: 142 LETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKH 201

Query: 184 IKAAVRSKIVNIAQLTSNAISLINRIAE 201
           ++ A+    V++ ++ SNA+++I  + +
Sbjct: 202 VRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of CmaCh04G003320 vs. TrEMBL
Match: A0A0A0LB27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 6.3e-75
Identity = 160/202 (79.21%), Postives = 177/202 (87.62%), Query Frame = 1


Query: 1   MGFSICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSL 60
           MG S  K++LIL   I QLTL    IHSAITPQSS    TEFIK+SCSSTTYPRLCFSSL
Sbjct: 1   MGISNSKKTLILFQLILQLTL----IHSAITPQSS----TEFIKSSCSSTTYPRLCFSSL 60

Query: 61  SLHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDS 120
           S+HA +IQTSPRLLATAALSVS+SSVKS  TQILKLSH+HGL  RDVSAL+DCLEEL DS
Sbjct: 61  SVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDS 120

Query: 121 IDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSK 180
           +DSL +SISEMPKLRG +FD+ MSN+QTWVSAALTDETTCSEGFQGKTVNGG+K AVR+K
Sbjct: 121 VDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTK 180

Query: 181 IVNIAQLTSNAISLINRIAELH 203
           IVNIAQLTSNA+SLINRIA+LH
Sbjct: 181 IVNIAQLTSNALSLINRIADLH 194

[Close]

BLAST of CmaCh04G003320 vs. TrEMBL
Match: A0A0A0L8D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 2.4e-74
Identity = 159/201 (79.10%), Postives = 175/201 (87.06%), Query Frame = 1


Query: 2   GFSICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLS 61
           G S  K+SLIL   I QLTL    IHSAITPQSS    TEFIK+SCSSTTYPRLCFSSLS
Sbjct: 44  GISNSKKSLILFQLILQLTL----IHSAITPQSS----TEFIKSSCSSTTYPRLCFSSLS 103

Query: 62  LHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSI 121
           +HA +IQTSPRLLATAALSVS+SSVKS  TQILKLSH+HGL  RDVSAL+DCLEEL DS+
Sbjct: 104 VHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSV 163

Query: 122 DSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKI 181
           DSL +SISEMPKLRG +FD+ MSN+QTWVSAALTDETTCSEGFQGKTVNGG+K AVR+KI
Sbjct: 164 DSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKI 223

Query: 182 VNIAQLTSNAISLINRIAELH 203
           VNIAQLTSNA+SLINRI +LH
Sbjct: 224 VNIAQLTSNALSLINRIGDLH 236

[Close]

BLAST of CmaCh04G003320 vs. TrEMBL
Match: M5XKR0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 7.0e-50
Identity = 104/165 (63.03%), Postives = 136/165 (82.42%), Query Frame = 1


Query: 38  SSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAALSVSISSVKSATTQILKLS 97
           ++TEFIKTSCS+TTYP+LC +SLS HA++IQTSP+L+A AAL+V++SS KS +  +LKLS
Sbjct: 38  TNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTLSSAKSTSAVMLKLS 97

Query: 98  HAHGLSPRDVSALNDCLEELGDSIDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDE 157
            +HGL P++V A+ DCLEEL DS+D L SSI+EM   +  DF +++S++QTWVSAALTDE
Sbjct: 98  QSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMISDVQTWVSAALTDE 157

Query: 158 TTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISLINRIAELH 203
            TCSEGF G  +NG +K AVR +IVNIAQLTSNA++LINR A +H
Sbjct: 158 NTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of CmaCh04G003320 vs. TrEMBL
Match: A0A061G793_THECC (DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 5.0e-48
Identity = 106/199 (53.27%), Postives = 148/199 (74.37%), Query Frame = 1


Query: 4   SICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLSLH 63
           + C  S   L+ +  L +N     S+   Q +  SS EFI+TSCSSTTYP+LCF +LS H
Sbjct: 4   TFCSHSFKFLLIL--LAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLSTH 63

Query: 64  ATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDS 123
           A+ IQTSP+LLA AAL+V++S+ +S +  ++ LS +HGL PR+  A+ DC+EEL DSID 
Sbjct: 64  ASLIQTSPQLLAHAALNVTLSTTESTSAMMVTLSKSHGLKPREAEAMQDCVEELSDSIDE 123

Query: 124 LISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVN 183
           L  SISEM +++G++F ++++++QTWVSAALTDE+TCS+GFQG  +NG +K AVR+KI+ 
Sbjct: 124 LRKSISEMGQVKGSNFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTKILR 183

Query: 184 IAQLTSNAISLINRIAELH 203
           IA LTSNA++LIN  A LH
Sbjct: 184 IAHLTSNALALINNYASLH 200

[Close]

BLAST of CmaCh04G003320 vs. TrEMBL
Match: B9ICT5_POPTR (Ripening-related family protein OS=Populus trichocarpa GN=POPTR_0015s14700g PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 3.3e-47
Identity = 104/190 (54.74%), Postives = 147/190 (77.37%), Query Frame = 1


Query: 10  LILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLSLHATSIQT 69
           L+LL+AI        +++S+   + ++ SSTEFI+TSCS+TTYPRLC++SLS+H+++IQT
Sbjct: 13  LLLLIAISS------YLNSSSAARVTTKSSTEFIRTSCSTTTYPRLCYTSLSIHSSTIQT 72

Query: 70  SPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDSLISSIS 129
           SP+LLA AAL+V++SS KS +T I  LS  H + PR+VSA+ DC+EELGD+++ L  S+ 
Sbjct: 73  SPKLLANAALNVTLSSAKSTSTMISTLSQTHRMKPREVSAMKDCVEELGDAVNELGKSMD 132

Query: 130 EMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTS 189
           EM   R ++F  ++ ++QTWVSAALTDE+TCS+GF G  +NG +K AVR +IVNIAQLTS
Sbjct: 133 EMSHARQSNFQSMIDDVQTWVSAALTDESTCSDGFAGNAMNGNLKTAVRGRIVNIAQLTS 192

Query: 190 NAISLINRIA 200
           NA++LIN  A
Sbjct: 193 NALALINNYA 196

[Close]

BLAST of CmaCh04G003320 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 179.5 bits (454), Expect = 2.1e-45
Identity = 92/194 (47.42%), Postives = 150/194 (77.32%), Query Frame = 1


Query: 9   SLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLSLHATSIQ 68
           + ++++A+ +L      +H+  T  ++++++TEF+K+SC+ TTYPRLCFSSLS HA+ IQ
Sbjct: 16  TFLIIIAMLKL------VHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQ 75

Query: 69  TSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDSLISSI 128
           TSP+L+A AAL+++++S K  +  +++LS++  L P++VSA+ DC+EELGD+++ L  SI
Sbjct: 76  TSPKLMAHAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSI 135

Query: 129 SEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQLT 188
            EM +L G++++V +S+IQTWVSAALTD  TC++GF+G+ ++G +K  VR +I+ IA LT
Sbjct: 136 GEMCQLSGSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLT 195

Query: 189 SNAISLINRIAELH 203
           SNA++LIN  A +H
Sbjct: 196 SNALALINHFASIH 202

[Close]

BLAST of CmaCh04G003320 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 151.4 bits (381), Expect = 6.1e-37
Identity = 88/195 (45.13%), Postives = 126/195 (64.62%), Query Frame = 1


Query: 12  LLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSP 71
           L + +F L L+   I  A   +  SS  TEFIK SC +T+YP  CF SLS +A+ I+  P
Sbjct: 7   LSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQP 66

Query: 72  RLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDSLISSISEM 131
           R LA  AL+VSI+  KSA T + +++   G++ R   A+ DCLEE+GD++D L +S+ E+
Sbjct: 67  RKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKEL 126

Query: 132 PKLR----GNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQL 191
             L     G DF   +SN++TW SAALTDET C +GF GK + G +K+ +R+ IV++A+ 
Sbjct: 127 KHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEE 186

Query: 192 TSNAISLINRIAELH 203
           TSNA++LIN  A  H
Sbjct: 187 TSNALALINDFASKH 201

[Close]

BLAST of CmaCh04G003320 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 148.3 bits (373), Expect = 5.2e-36
Identity = 87/203 (42.86%), Postives = 131/203 (64.53%), Query Frame = 1


Query: 5   ICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLSLHA 64
           + KQ L +L++I  L    +   +A +   +S  +  FI++SC +TTYP LC  SLS++A
Sbjct: 1   MAKQYLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYA 60

Query: 65  TSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDSL 124
             IQTSP+ LA  A++V++S  +S    + +L+   GL  R+V A+ DC+EE+ D++D L
Sbjct: 61  NDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRL 120

Query: 125 ISSISEMPKLRG-----NDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRS 184
             S+ E+ KL G     + F   MSN QTW SAALTDE TCS+GF G+ ++G IK +VR+
Sbjct: 121 TKSVQEL-KLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRA 180

Query: 185 KIVNIAQLTSNAISLINRIAELH 203
           +I+N+   TSNA+SLIN  A+ +
Sbjct: 181 RIMNVGHETSNALSLINAFAKTY 202

[Close]

BLAST of CmaCh04G003320 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 135.6 bits (340), Expect = 3.5e-32
Identity = 79/180 (43.89%), Postives = 126/180 (70.00%), Query Frame = 1


Query: 28  SAITPQSSSSS-----STEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAALSVS 87
           S+++P SSSS+     + ++IKTSC+ T Y  +C++SLS +A++I+++P+ LA  AL+++
Sbjct: 128 SSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLT 187

Query: 88  ISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDSLISSISEMPKLRGND---F 147
           +SS KSA+  +  +SH  GL+  +V A+ DC+EE+GDS+ SL  SI E+  +   D   F
Sbjct: 188 LSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKF 247

Query: 148 DVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISLINRIA 200
           ++VMS+++TWVSAALT++ TC +GF    V   +K  VR  +V +A+LTSNA++LIN  A
Sbjct: 248 EMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of CmaCh04G003320 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 131.0 bits (328), Expect = 8.5e-31
Identity = 79/188 (42.02%), Postives = 118/188 (62.77%), Query Frame = 1


Query: 23  PIFIHSAITPQSSS---SSSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAAL 82
           P+   S I   SSS   SSS  FI +SC  T Y  LC   L+  A  I+ +   LA  AL
Sbjct: 17  PLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTAL 76

Query: 83  SVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDSLISSISEMPKL--RGN 142
           +V++  V+S T  + KL+ A  +  R+  A+ DC+E LGD ++ L  S+ E+ ++   G 
Sbjct: 77  AVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSGR 136

Query: 143 DFDVVM---SNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISL 202
           D D  +   SN++TWVSAALTDETTC +GF GK ++G +K+A+R ++V++A++TSNA++L
Sbjct: 137 DRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALAL 196

[Close]

BLAST of CmaCh04G003320 vs. NCBI nr
Match: gi|778679325|ref|XP_004148138.2| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 288.5 bits (737), Expect = 9.0e-75
Identity = 160/202 (79.21%), Postives = 177/202 (87.62%), Query Frame = 1


Query: 1   MGFSICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSL 60
           MG S  K++LIL   I QLTL    IHSAITPQSS    TEFIK+SCSSTTYPRLCFSSL
Sbjct: 1   MGISNSKKTLILFQLILQLTL----IHSAITPQSS----TEFIKSSCSSTTYPRLCFSSL 60

Query: 61  SLHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDS 120
           S+HA +IQTSPRLLATAALSVS+SSVKS  TQILKLSH+HGL  RDVSAL+DCLEEL DS
Sbjct: 61  SVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDS 120

Query: 121 IDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSK 180
           +DSL +SISEMPKLRG +FD+ MSN+QTWVSAALTDETTCSEGFQGKTVNGG+K AVR+K
Sbjct: 121 VDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTK 180

Query: 181 IVNIAQLTSNAISLINRIAELH 203
           IVNIAQLTSNA+SLINRIA+LH
Sbjct: 181 IVNIAQLTSNALSLINRIADLH 194

[Close]

BLAST of CmaCh04G003320 vs. NCBI nr
Match: gi|778688522|ref|XP_011652768.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 286.6 bits (732), Expect = 3.4e-74
Identity = 159/201 (79.10%), Postives = 175/201 (87.06%), Query Frame = 1


Query: 2   GFSICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLS 61
           G S  K+SLIL   I QLTL    IHSAITPQSS    TEFIK+SCSSTTYPRLCFSSLS
Sbjct: 44  GISNSKKSLILFQLILQLTL----IHSAITPQSS----TEFIKSSCSSTTYPRLCFSSLS 103

Query: 62  LHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSI 121
           +HA +IQTSPRLLATAALSVS+SSVKS  TQILKLSH+HGL  RDVSAL+DCLEEL DS+
Sbjct: 104 VHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSV 163

Query: 122 DSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKI 181
           DSL +SISEMPKLRG +FD+ MSN+QTWVSAALTDETTCSEGFQGKTVNGG+K AVR+KI
Sbjct: 164 DSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKI 223

Query: 182 VNIAQLTSNAISLINRIAELH 203
           VNIAQLTSNA+SLINRI +LH
Sbjct: 224 VNIAQLTSNALSLINRIGDLH 236

[Close]

BLAST of CmaCh04G003320 vs. NCBI nr
Match: gi|596006342|ref|XP_007218426.1| (hypothetical protein PRUPE_ppa011607mg [Prunus persica])

HSP 1 Score: 205.3 bits (521), Expect = 1.0e-49
Identity = 104/165 (63.03%), Postives = 136/165 (82.42%), Query Frame = 1


Query: 38  SSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAALSVSISSVKSATTQILKLS 97
           ++TEFIKTSCS+TTYP+LC +SLS HA++IQTSP+L+A AAL+V++SS KS +  +LKLS
Sbjct: 38  TNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTLSSAKSTSAVMLKLS 97

Query: 98  HAHGLSPRDVSALNDCLEELGDSIDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDE 157
            +HGL P++V A+ DCLEEL DS+D L SSI+EM   +  DF +++S++QTWVSAALTDE
Sbjct: 98  QSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMISDVQTWVSAALTDE 157

Query: 158 TTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISLINRIAELH 203
            TCSEGF G  +NG +K AVR +IVNIAQLTSNA++LINR A +H
Sbjct: 158 NTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of CmaCh04G003320 vs. NCBI nr
Match: gi|645257371|ref|XP_008234384.1| (PREDICTED: 21 kDa protein [Prunus mume])

HSP 1 Score: 202.6 bits (514), Expect = 6.5e-49
Identity = 103/165 (62.42%), Postives = 135/165 (81.82%), Query Frame = 1


Query: 38  SSTEFIKTSCSSTTYPRLCFSSLSLHATSIQTSPRLLATAALSVSISSVKSATTQILKLS 97
           ++TEFIKTSC +TTYP+LC +SLS HA++IQTSP+L+A AAL+V++SS KS +  +LKLS
Sbjct: 38  TNTEFIKTSCRATTYPKLCRTSLSSHASTIQTSPKLMAGAALNVTLSSAKSTSATMLKLS 97

Query: 98  HAHGLSPRDVSALNDCLEELGDSIDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDE 157
            +HGL P++V A+ DCLEEL DS+D L SSI+EM   +  DF +++S++QTWVSAALTDE
Sbjct: 98  QSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMISDVQTWVSAALTDE 157

Query: 158 TTCSEGFQGKTVNGGIKAAVRSKIVNIAQLTSNAISLINRIAELH 203
            TCSEGF G  +NG +K AVR +IVNIAQLTSNA++LINR A +H
Sbjct: 158 NTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of CmaCh04G003320 vs. NCBI nr
Match: gi|590671750|ref|XP_007038418.1| (DC1.2-like, putative [Theobroma cacao])

HSP 1 Score: 199.1 bits (505), Expect = 7.2e-48
Identity = 106/199 (53.27%), Postives = 148/199 (74.37%), Query Frame = 1


Query: 4   SICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSLSLH 63
           + C  S   L+ +  L +N     S+   Q +  SS EFI+TSCSSTTYP+LCF +LS H
Sbjct: 4   TFCSHSFKFLLIL--LAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLSTH 63

Query: 64  ATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSIDS 123
           A+ IQTSP+LLA AAL+V++S+ +S +  ++ LS +HGL PR+  A+ DC+EEL DSID 
Sbjct: 64  ASLIQTSPQLLAHAALNVTLSTTESTSAMMVTLSKSHGLKPREAEAMQDCVEELSDSIDE 123

Query: 124 LISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSKIVN 183
           L  SISEM +++G++F ++++++QTWVSAALTDE+TCS+GFQG  +NG +K AVR+KI+ 
Sbjct: 124 LRKSISEMGQVKGSNFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTKILR 183

Query: 184 IAQLTSNAISLINRIAELH 203
           IA LTSNA++LIN  A LH
Sbjct: 184 IAHLTSNALALINNYASLH 200

The following BLAST results are available for this feature:

BLAST of CmaCh04G003320 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	2.2e-36	47.73	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME2_ARATH	6.8e-14	30.77	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2	[more]
PME3_PHAVU	2.4e-11	31.37	Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1	[more]
PME3_ARATH	5.4e-11	27.91	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S...	[more]
PME1_CITSI	2.7e-10	25.96	Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1	[more]

BLAST of CmaCh04G003320 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB27_CUCSA	6.3e-75	79.21	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1	[more]
A0A0A0L8D1_CUCSA	2.4e-74	79.10	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1	[more]
M5XKR0_PRUPE	7.0e-50	63.03	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1	[more]
A0A061G793_THECC	5.0e-48	53.27	DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1	[more]
B9ICT5_POPTR	3.3e-47	54.74	Ripening-related family protein OS=Populus trichocarpa GN=POPTR_0015s14700g PE=4...	[more]

BLAST of CmaCh04G003320 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62360.1	2.1e-45	47.42	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	6.1e-37	45.13	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	5.2e-36	42.86	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	3.5e-32	43.89	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	8.5e-31	42.02	pectin methylesterase inhibitor 1	[more]

BLAST of CmaCh04G003320 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778679325\|ref\|XP_004148138.2\|	9.0e-75	79.21	PREDICTED: 21 kDa protein [Cucumis sativus]	[more]
gi\|778688522\|ref\|XP_011652768.1\|	3.4e-74	79.10	PREDICTED: 21 kDa protein-like [Cucumis sativus]	[more]
gi\|596006342\|ref\|XP_007218426.1\|	1.0e-49	63.03	hypothetical protein PRUPE_ppa011607mg [Prunus persica]	[more]
gi\|645257371\|ref\|XP_008234384.1\|	6.5e-49	62.42	PREDICTED: 21 kDa protein [Prunus mume]	[more]
gi\|590671750\|ref\|XP_007038418.1\|	7.2e-48	53.27	DC1.2-like, putative [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G003320.1	CmaCh04G003320.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 39..198 score: 4.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 42..194 score: 1.8
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 38..194 score: 5.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 20..197 score: 5.1
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 41..198 score: 1.7
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 2..202 score: 5.4
None	No IPR available	PANTHER	PTHR31080:SF19	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 2..202 score: 5.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh04G003320	MELO3C006819	Melon (DHL92) v3.5.1	cmameB692
CmaCh04G003320	Cp4.1LG14g05770	Cucurbita pepo (Zucchini)	cmacpeB715
CmaCh04G003320	MELO3C006819.2	Melon (DHL92) v3.6.1	cmamedB779

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh04G003320	Watermelon (97103) v2	cmawmbB767
CmaCh04G003320	Wax gourd	cmawgoB0856
CmaCh04G003320	Cucurbita maxima (Rimu)	cmacmaB351
CmaCh04G003320	Cucumber (Gy14) v1	cgycmaB0722
CmaCh04G003320	Cucurbita moschata (Rifu)	cmacmoB701
CmaCh04G003320	Cucurbita moschata (Rifu)	cmacmoB728
CmaCh04G003320	Wild cucumber (PI 183967)	cmacpiB712
CmaCh04G003320	Cucumber (Chinese Long) v2	cmacuB703
CmaCh04G003320	Watermelon (Charleston Gray)	cmawcgB663
CmaCh04G003320	Watermelon (97103) v1	cmawmB707
CmaCh04G003320	Cucurbita pepo (Zucchini)	cmacpeB720
CmaCh04G003320	Bottle gourd (USVL1VR-Ls)	cmalsiB688
CmaCh04G003320	Cucumber (Gy14) v2	cgybcmaB417
CmaCh04G003320	Silver-seed gourd	carcmaB0529
CmaCh04G003320	Silver-seed gourd	carcmaB1209
CmaCh04G003320	Cucumber (Chinese Long) v3	cmacucB0838