CmoCh05G009280 (gene) Cucurbita moschata (Rifu)

NameCmoCh05G009280
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionPectinesterase (3.1.1.11)
LocationCmo_Chr05 : 7255594 .. 7256665 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTAGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACAGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTGAGCACGAAAAGATTTTCCATTCATGTGAAACCTGGAACATACAAGGAACTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATCATCGTTGACGATCGAAGTAATGGCACTGGATCGACCACAGCAACTTCTGCAACTCTCAGTAAGCTTCAAATTCTCCACTAGCCGATTAAATTTGTGTTAATACTAAAACATATTTCATTTTATAGCTGTAAGAGGTGCAAATTTCATGGCTCAGTCGTTGACATTTCAAAACTCAGCTGGGCCGAAGGAGCACCAAGCTGTTGCAGTTCTTGACCAAGCCAAACACTCCGCATACTATAAGTGTGTGTTTTTGGGTTATCAAGATACGCTTTATGCTGGGGGACTACCCCAATTCTTCAGAGAGTGCGATATATACGGAAGCATTGACTTCATATTCGGAAACGGAGTCACAGTATTCCAAGATTGTAATATATATGTTCGAGAATTTGGTATTAAGACTACGGTAACAGCACAATCGAAAGCAAATATAGATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTCATCGAGTCATTTCTTGACACTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAGTGGTTGCCTGAAACTGGCATTCCATTTAGAACTGGTTTTTAG

mRNA sequence

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTAGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACAGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTGAGCACGAAAAGATTTTCCATTCATGTGAAACCTGGAACATACAAGGAACTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATCATCGTTGACGATCGAAGTAATGGCACTGGATCGACCACAGCAACTTCTGCAACTCTCACTGTAAGAGGTGCAAATTTCATGGCTCAGTCGTTGACATTTCAAAACTCAGCTGGGCCGAAGGAGCACCAAGCTGTTGCAGTTCTTGACCAAGCCAAACACTCCGCATACTATAAGTGTGTGTTTTTGGGTTATCAAGATACGCTTTATGCTGGGGGACTACCCCAATTCTTCAGAGAGTGCGATATATACGGAAGCATTGACTTCATATTCGGAAACGGAGTCACAGTATTCCAAGATTGTAATATATATGTTCGAGAATTTGGTATTAAGACTACGGTAACAGCACAATCGAAAGCAAATATAGATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTCATCGAGTCATTTCTTGACACTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAGTGGTTGCCTGAAACTGGCATTCCATTTAGAACTGGTTTTTAG

Coding sequence (CDS)

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTAGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACAGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTGAGCACGAAAAGATTTTCCATTCATGTGAAACCTGGAACATACAAGGAACTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATCATCGTTGACGATCGAAGTAATGGCACTGGATCGACCACAGCAACTTCTGCAACTCTCACTGTAAGAGGTGCAAATTTCATGGCTCAGTCGTTGACATTTCAAAACTCAGCTGGGCCGAAGGAGCACCAAGCTGTTGCAGTTCTTGACCAAGCCAAACACTCCGCATACTATAAGTGTGTGTTTTTGGGTTATCAAGATACGCTTTATGCTGGGGGACTACCCCAATTCTTCAGAGAGTGCGATATATACGGAAGCATTGACTTCATATTCGGAAACGGAGTCACAGTATTCCAAGATTGTAATATATATGTTCGAGAATTTGGTATTAAGACTACGGTAACAGCACAATCGAAAGCAAATATAGATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTCATCGAGTCATTTCTTGACACTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAGTGGTTGCCTGAAACTGGCATTCCATTTAGAACTGGTTTTTAG
BLAST of CmoCh05G009280 vs. Swiss-Prot
Match: PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=PME39 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.7e-81
Identity = 145/314 (46.18%), Postives = 206/314 (65.61%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDD 83
           +++++V++DGTGN+  I+DA+ AAPN+S  RF I++K G Y E +E+P  KT I  IGD 
Sbjct: 219 NYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDG 278

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 143
              T+I  +RS   G +T  + T+ V+G  ++A+ ++F NSAGP + QAVA    + HSA
Sbjct: 279 IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSA 338

Query: 144 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVRE--FGIKTTV 203
           +Y+C F GYQDTLY     QF+RECDIYG+IDFIFGN   VFQ+ ++Y R+   G K   
Sbjct: 339 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 398

Query: 204 TAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQ 263
           TAQS+   D  +G    NC++  +P++   K+    YLGRPW++YS  V I+SF+D ++ 
Sbjct: 399 TAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIH 458

Query: 264 PKGWL-GWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGS 323
           P GWL G  D  ++ L++ EY N GPGA+ ++RV WPG+  +   T+ T+FTV  FI GS
Sbjct: 459 PAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGS 518

Query: 324 QWLPETGIPFRTGF 335
            WL  TGIPF  GF
Sbjct: 519 TWLNSTGIPFSLGF 532

BLAST of CmoCh05G009280 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.7e-81
Identity = 143/310 (46.13%), Postives = 206/310 (66.45%), Query Frame = 1

Query: 26  DVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDDAS 85
           ++ V+ DG+GN   I +A+AAA      R+ I++K GTY E IEV      I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 86  TTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSAYY 145
            TII   +S G G+TT  SAT+ V G NF+A+ +T +N+AGP  HQAVA+   +  S +Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 146 KCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVREFGIKT-TVTAQ 205
           +C F GYQDTLY     QF+RECDIYG++DFIFGN   V Q+CNI+ R+   +T T+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 206 SKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQPKG 265
            + + +  +G +  NC+VT + ++   +  V  +LGRPWK+YS  V+I++FLD+++ P G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 266 WLGWP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWL 325
           W+ W  D  ++ L++AEY NTGPG+ T+ RV W GYH+L + +QV++FTV NFI G+ WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 326 PETGIPFRTG 334
           P T +PF +G
Sbjct: 502 PATNVPFTSG 509

BLAST of CmoCh05G009280 vs. Swiss-Prot
Match: PME44_ARATH (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=PME44 PE=2 SV=2)

HSP 1 Score: 293.9 bits (751), Expect = 2.2e-78
Identity = 142/314 (45.22%), Postives = 199/314 (63.38%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDD 83
           ++DV+V+LDGTGN+ KI DAI  AP+ S+ RF I++K G Y E +E+   K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 143
              T+I  +RS   G TT  SAT  V G  F+A+ +TFQN+AGP++HQAVA+   +  S 
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 329

Query: 144 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVREFGI---KTT 203
           +++C   GYQDTLY   + QF+REC I G++DFIFG+G  VFQ+C I  +  G+   K T
Sbjct: 330 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKR-GLPNQKNT 389

Query: 204 VTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVV 263
           +TAQ + +++  SGF  Q   ++   ++    +    YLGRPWK YS  VFI + +  VV
Sbjct: 390 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVV 449

Query: 264 QPKGWLGW-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGG 323
           +P+GWL W  D  +D LF+ E+ N GPG+  S RV WPGYH+ + + Q   FTV  FI G
Sbjct: 450 RPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKG 509

Query: 324 SQWLPETGIPFRTG 334
           + WLP TG+ F  G
Sbjct: 510 NLWLPSTGVTFSDG 522

BLAST of CmoCh05G009280 vs. Swiss-Prot
Match: PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 2.9e-78
Identity = 140/312 (44.87%), Postives = 195/312 (62.50%), Query Frame = 1

Query: 26  DVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDDAS 85
           D  V+ DGTGN+  ISDA+ AAP+ STKR+ IHVK G Y E +E+   K  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 86  TTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSAYY 145
            T+I  +RS   G TT  SAT  V G  F+A+ +TFQN+AGP++HQAVA+        +Y
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFY 333

Query: 146 KCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVREFGI---KTTVT 205
           +C   GYQDTLYA  + QFFREC I G++DFIFG+   VFQ C I  ++ G+   K ++T
Sbjct: 334 RCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQ-GLPNQKNSIT 393

Query: 206 AQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQP 265
           AQ + + ++ +GF  Q   +    ++  + +    YLGRPWK YS  VF+++++   + P
Sbjct: 394 AQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINP 453

Query: 266 KGWLGW-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQ 325
            GWL W  +  +D L++ EY N+GPGA   RRV WPGYH+L+T+ +   FTV   I G+ 
Sbjct: 454 VGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNL 513

Query: 326 WLPETGIPFRTG 334
           WLP TGI F  G
Sbjct: 514 WLPSTGITFIAG 524

BLAST of CmoCh05G009280 vs. Swiss-Prot
Match: PME20_ARATH (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=PME20 PE=2 SV=2)

HSP 1 Score: 290.4 bits (742), Expect = 2.5e-77
Identity = 142/313 (45.37%), Postives = 200/313 (63.90%), Query Frame = 1

Query: 27  VTVSLDGTGNYVKISDAIAAAPNL---STKRFSIHVKPGTYKELIEVPPSKTFIALIGDD 86
           VTV  +GTGN+  I+ AIAAAPN    S   F I+V  G Y+E +EVP +K ++ +IGD 
Sbjct: 246 VTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDG 305

Query: 87  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 146
            + T+I  +RS   G TT  SAT  + G NF+  ++T +N+AGP + QAVA+      S 
Sbjct: 306 INQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSV 365

Query: 147 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVRE--FGIKTTV 206
           +Y C F  YQDTLY   L QF+RECD+YG++DFIFGN   V Q+CN+Y R+   G    V
Sbjct: 366 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEV 425

Query: 207 TAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQ 266
           TAQ + + +  +G     C +  + ++A+S   V  YLGRPWKEYS  V +++++D  ++
Sbjct: 426 TAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLE 485

Query: 267 PKGWLGWP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGS 326
           P GW  W  D  +  L++AEYNNTGPG+DT+ RV WPGYH+++ AT  + FTV NF+ G 
Sbjct: 486 PSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVIN-ATDASNFTVTNFLVGE 545

Query: 327 QWLPETGIPFRTG 334
            W+ +TG+PF  G
Sbjct: 546 GWIGQTGVPFVGG 557

BLAST of CmoCh05G009280 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 9.2e-140
Identity = 241/330 (73.03%), Postives = 285/330 (86.36%), Query Frame = 1

Query: 8   MLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTY 67
           M+F++YLLV     FS C+SF+ TVSLDGT N++KI+DAIAAAPN ST RF IHVKPGTY
Sbjct: 1   MIFVLYLLVVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTY 60

Query: 68  KELIEVPPSKTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNS 127
           +E+IEVP  KT IALIGDD+STTIIV++RSNGTGS+TA+SATLTV G+NFMAQ LTFQN+
Sbjct: 61  QEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNT 120

Query: 128 AGPKEHQAVAVLDQAKHSAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTV 187
           AGP E QA+A+LDQA H+AYYKCVFLGYQDTLYAG LPQFF+ECDIYGS+DFIFGNG+ V
Sbjct: 121 AGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVV 180

Query: 188 FQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWK 247
           FQDCNIY R F ++ TVTAQSK +++  SGF+FQNCKVTVSPEIASSKDKV ++LGRPW+
Sbjct: 181 FQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWR 240

Query: 248 EYSTVVFIESFLDTVVQPKGWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHT 307
            YS VVFI+SFLD VVQPKGWL WP VP + L++AEYNN+G GA+TS+RV+WPGYH L+ 
Sbjct: 241 MYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNN 300

Query: 308 ATQVTRFTVENFIGGSQWLPETGIPFRTGF 335
           A +V  FTVE FI G+QWLP+TGIPFR GF
Sbjct: 301 AKEVATFTVEIFINGTQWLPQTGIPFRAGF 330

BLAST of CmoCh05G009280 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 1.8e-111
Identity = 199/310 (64.19%), Postives = 241/310 (77.74%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDD 83
           SF++TVSLDG+GN+ +ISDAIAAAPN S  RF IHV PGTY E +++P  K FIAL+GDD
Sbjct: 25  SFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDD 84

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 143
             TTII+D+RSNGTG  T  SATLTV+G NFMA+SLTF+NSAGP+ +QAVAV D+A H+A
Sbjct: 85  PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTA 144

Query: 144 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVREFGIKTTVTA 203
           YYKC FL +QDTLY  G PQFF+E DIYGS+DFI G G  +FQDCNIY R      TVTA
Sbjct: 145 YYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTA 204

Query: 204 QSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQPK 263
           QSK  +   SGF FQNC VTVS EI+S+K  V ++LGRPWK+YS VVF+ESFLD VV  +
Sbjct: 205 QSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASE 264

Query: 264 GWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWL 323
           GW+ W  VPV+ LF+ E+NN GPGAD S+RV+W  YH+L   + + RFTV+NF+ GS+WL
Sbjct: 265 GWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESAL-RFTVDNFVNGSEWL 324

Query: 324 PETGIPFRTG 334
           PETGIPFR G
Sbjct: 325 PETGIPFRRG 333

BLAST of CmoCh05G009280 vs. TrEMBL
Match: A0A151U4L2_CAJCA (Pectinesterase OS=Cajanus cajan GN=KK1_006907 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-83
Identity = 150/312 (48.08%), Postives = 209/312 (66.99%), Query Frame = 1

Query: 25  FDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDDA 84
           FD+ V+ DGTGN+  I +A+A APN ST RF IH+K G Y E +EV   KT +  +GD  
Sbjct: 157 FDLVVAKDGTGNFTTIGEALAVAPNSSTTRFVIHIKAGAYLENVEVIRKKTNLMFVGDGI 216

Query: 85  STTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSAY 144
             T++   R+   G TT  SAT+ V GA F+A+ +TF+NSAGP++HQAVA+   A  SA+
Sbjct: 217 GKTVVKGSRNVVDGWTTFQSATVAVVGAGFIAKGITFENSAGPEKHQAVALRSGADFSAF 276

Query: 145 YKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVRE--FGIKTTVT 204
           Y+C F+GYQDTLY   L QF+RECDIYG++DFIFGN   VFQ+CN+Y R+     K   T
Sbjct: 277 YQCSFVGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYARKPNQNQKNLFT 336

Query: 205 AQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQP 264
           AQ + + +  +G    NCKV  + ++   K     YLGRPWK YS  VF++S+++ +++P
Sbjct: 337 AQGREDPNQNTGISILNCKVAAAADLIPVKTSFKSYLGRPWKMYSRTVFLKSYMEDLIEP 396

Query: 265 KGWLGWPDV-PVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQ 324
            GWL W D   +D L++ EY N GPG++TS RV WPGY +++++T+ ++FTV  FI G+ 
Sbjct: 397 AGWLEWNDTFALDTLYYGEYMNRGPGSNTSGRVTWPGYRVINSSTEASQFTVGEFIQGND 456

Query: 325 WLPETGIPFRTG 334
           WL  TGIPF +G
Sbjct: 457 WLNSTGIPFFSG 468

BLAST of CmoCh05G009280 vs. TrEMBL
Match: A0A0B0P3D3_GOSAR (Pectinesterase OS=Gossypium arboreum GN=F383_07269 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.6e-83
Identity = 154/322 (47.83%), Postives = 216/322 (67.08%), Query Frame = 1

Query: 15  LVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNLS-TKRFSIHVKPGTYKELIEVPPS 74
           L+ + S   + ++ V+ DG+GNY  I DAI+AA   S + R+ I+VK GTYKE +E+   
Sbjct: 197 LLQSSSPASTANIVVAQDGSGNYKTIKDAISAASKRSGSGRYVIYVKAGTYKENVEIGSK 256

Query: 75  KTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAV 134
              I ++GD    TII   +S G GSTT  SAT+ V G  F+A+ +TF+N+AGP  HQAV
Sbjct: 257 LKNIMMVGDGIGKTIITGSKSVGGGSTTFNSATVAVVGDGFIARGITFRNTAGPTNHQAV 316

Query: 135 AVLDQAKHSAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVR 194
           A+   +  S +YKC F GYQDTLY     QF+RECDIYG++D+IFGN   VFQDCNIY R
Sbjct: 317 ALRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRECDIYGTVDWIFGNAAVVFQDCNIYAR 376

Query: 195 EFGIKT-TVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFI 254
               KT TVTAQ + + +  +G +  N +VT + ++   +  V  YLGRPWK+YS  VF+
Sbjct: 377 NPPNKTNTVTAQGRTDPNQNTGIIIHNSRVTAASDLKPVQSSVKTYLGRPWKQYSRTVFM 436

Query: 255 ESFLDTVVQPKGWLGWP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRF 314
           +++LD+++ P GW+ W  D  +  L++AEY NTGPG+ TS RV W GYH+L +A++V++F
Sbjct: 437 KTYLDSLINPAGWMEWDGDFALKTLYYAEYMNTGPGSSTSNRVKWGGYHVLKSASEVSKF 496

Query: 315 TVENFIGGSQWLPETGIPFRTG 334
           TV NF+ G+ WLP TG+PF +G
Sbjct: 497 TVGNFLAGNSWLPSTGVPFTSG 518

BLAST of CmoCh05G009280 vs. TrEMBL
Match: R9QQU4_GOSBA (Pectinesterase OS=Gossypium barbadense PE=2 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.4e-82
Identity = 153/322 (47.52%), Postives = 216/322 (67.08%), Query Frame = 1

Query: 15  LVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNLS-TKRFSIHVKPGTYKELIEVPPS 74
           L+ + S   + ++ V+ DG+GNY  I DAI+AA   S + R+ I+VK GTYKE +E+   
Sbjct: 197 LLQSSSPASTANIVVAQDGSGNYKTIKDAISAASKRSGSGRYVIYVKAGTYKENVEIGSK 256

Query: 75  KTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAV 134
              I ++GD    TII   +S G GSTT  SAT+ V G  F+A+ +TF+N+AGP  HQAV
Sbjct: 257 LKNIMMVGDGIGKTIITGSKSVGGGSTTFNSATVAVVGDGFIARGITFRNTAGPTNHQAV 316

Query: 135 AVLDQAKHSAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVR 194
           A+   +  S +YKC F GYQDTLY     QF+RECDIYG++D+IFGN   VFQ+CNIY R
Sbjct: 317 ALRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRECDIYGTVDWIFGNAAVVFQNCNIYAR 376

Query: 195 EFGIKT-TVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFI 254
               KT TVTAQ + + +  +G +  N +VT + ++   +  V  YLGRPWK+YS  VF+
Sbjct: 377 NPPNKTNTVTAQGRTDPNQNTGIIIHNSRVTAASDLKPVQSSVKTYLGRPWKQYSRTVFM 436

Query: 255 ESFLDTVVQPKGWLGWP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRF 314
           +++LD+++ P GW+ W  D  +  L++AEY NTGPG+ TS RV W GYH+L +A++V++F
Sbjct: 437 KTYLDSLINPAGWMEWDGDFALKTLYYAEYMNTGPGSSTSNRVKWGGYHVLKSASEVSKF 496

Query: 315 TVENFIGGSQWLPETGIPFRTG 334
           TV NF+ G+ WLP TG+PF +G
Sbjct: 497 TVGNFLAGNSWLPSTGVPFTSG 518

BLAST of CmoCh05G009280 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 303.1 bits (775), Expect = 2.1e-82
Identity = 145/314 (46.18%), Postives = 206/314 (65.61%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDD 83
           +++++V++DGTGN+  I+DA+ AAPN+S  RF I++K G Y E +E+P  KT I  IGD 
Sbjct: 219 NYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDG 278

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 143
              T+I  +RS   G +T  + T+ V+G  ++A+ ++F NSAGP + QAVA    + HSA
Sbjct: 279 IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSA 338

Query: 144 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVRE--FGIKTTV 203
           +Y+C F GYQDTLY     QF+RECDIYG+IDFIFGN   VFQ+ ++Y R+   G K   
Sbjct: 339 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 398

Query: 204 TAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQ 263
           TAQS+   D  +G    NC++  +P++   K+    YLGRPW++YS  V I+SF+D ++ 
Sbjct: 399 TAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIH 458

Query: 264 PKGWL-GWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGS 323
           P GWL G  D  ++ L++ EY N GPGA+ ++RV WPG+  +   T+ T+FTV  FI GS
Sbjct: 459 PAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGS 518

Query: 324 QWLPETGIPFRTGF 335
            WL  TGIPF  GF
Sbjct: 519 TWLNSTGIPFSLGF 532

BLAST of CmoCh05G009280 vs. TAIR10
Match: AT4G33220.1 (AT4G33220.1 pectin methylesterase 44)

HSP 1 Score: 293.9 bits (751), Expect = 1.3e-79
Identity = 142/314 (45.22%), Postives = 199/314 (63.38%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDD 83
           ++DV+V+LDGTGN+ KI DAI  AP+ S+ RF I++K G Y E +E+   K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 143
              T+I  +RS   G TT  SAT  V G  F+A+ +TFQN+AGP++HQAVA+   +  S 
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 329

Query: 144 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVREFGI---KTT 203
           +++C   GYQDTLY   + QF+REC I G++DFIFG+G  VFQ+C I  +  G+   K T
Sbjct: 330 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKR-GLPNQKNT 389

Query: 204 VTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVV 263
           +TAQ + +++  SGF  Q   ++   ++    +    YLGRPWK YS  VFI + +  VV
Sbjct: 390 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVV 449

Query: 264 QPKGWLGW-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGG 323
           +P+GWL W  D  +D LF+ E+ N GPG+  S RV WPGYH+ + + Q   FTV  FI G
Sbjct: 450 RPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKG 509

Query: 324 SQWLPETGIPFRTG 334
           + WLP TG+ F  G
Sbjct: 510 NLWLPSTGVTFSDG 522

BLAST of CmoCh05G009280 vs. TAIR10
Match: AT3G43270.1 (AT3G43270.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 293.5 bits (750), Expect = 1.6e-79
Identity = 140/312 (44.87%), Postives = 195/312 (62.50%), Query Frame = 1

Query: 26  DVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDDAS 85
           D  V+ DGTGN+  ISDA+ AAP+ STKR+ IHVK G Y E +E+   K  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 86  TTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSAYY 145
            T+I  +RS   G TT  SAT  V G  F+A+ +TFQN+AGP++HQAVA+        +Y
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFY 333

Query: 146 KCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVREFGI---KTTVT 205
           +C   GYQDTLYA  + QFFREC I G++DFIFG+   VFQ C I  ++ G+   K ++T
Sbjct: 334 RCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQ-GLPNQKNSIT 393

Query: 206 AQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQP 265
           AQ + + ++ +GF  Q   +    ++  + +    YLGRPWK YS  VF+++++   + P
Sbjct: 394 AQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINP 453

Query: 266 KGWLGW-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQ 325
            GWL W  +  +D L++ EY N+GPGA   RRV WPGYH+L+T+ +   FTV   I G+ 
Sbjct: 454 VGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNL 513

Query: 326 WLPETGIPFRTG 334
           WLP TGI F  G
Sbjct: 514 WLPSTGITFIAG 524

BLAST of CmoCh05G009280 vs. TAIR10
Match: AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 290.4 bits (742), Expect = 1.4e-78
Identity = 142/313 (45.37%), Postives = 200/313 (63.90%), Query Frame = 1

Query: 27  VTVSLDGTGNYVKISDAIAAAPNL---STKRFSIHVKPGTYKELIEVPPSKTFIALIGDD 86
           VTV  +GTGN+  I+ AIAAAPN    S   F I+V  G Y+E +EVP +K ++ +IGD 
Sbjct: 246 VTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDG 305

Query: 87  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 146
            + T+I  +RS   G TT  SAT  + G NF+  ++T +N+AGP + QAVA+      S 
Sbjct: 306 INQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSV 365

Query: 147 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVRE--FGIKTTV 206
           +Y C F  YQDTLY   L QF+RECD+YG++DFIFGN   V Q+CN+Y R+   G    V
Sbjct: 366 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEV 425

Query: 207 TAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQ 266
           TAQ + + +  +G     C +  + ++A+S   V  YLGRPWKEYS  V +++++D  ++
Sbjct: 426 TAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLE 485

Query: 267 PKGWLGWP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGS 326
           P GW  W  D  +  L++AEYNNTGPG+DT+ RV WPGYH+++ AT  + FTV NF+ G 
Sbjct: 486 PSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVIN-ATDASNFTVTNFLVGE 545

Query: 327 QWLPETGIPFRTG 334
            W+ +TG+PF  G
Sbjct: 546 GWIGQTGVPFVGG 557

BLAST of CmoCh05G009280 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 290.0 bits (741), Expect = 1.8e-78
Identity = 139/313 (44.41%), Postives = 204/313 (65.18%), Query Frame = 1

Query: 27  VTVSLDGTGNYVKISDAIAAAPNLSTKR---FSIHVKPGTYKELIEVPPSKTFIALIGDD 86
           VTV+ +GTGN+  I++A+ +APN +      F I+V  G Y+E + +  +K ++ +IGD 
Sbjct: 259 VTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDG 318

Query: 87  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 146
            + T++  +R+   G TT  SAT  V   NF+A ++TF+N+AGP++HQAVA+   A  S 
Sbjct: 319 INRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSI 378

Query: 147 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVRE--FGIKTTV 206
           +Y C F  YQDTLY   L QF+RECDIYG++DFIFGN   VFQDCN+Y R+        +
Sbjct: 379 FYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAI 438

Query: 207 TAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQ 266
           TAQ + + +  +G    NC +  + ++ SS   V  YLGRPWKEYS  VF++S++D VV+
Sbjct: 439 TAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVE 498

Query: 267 PKGWLGW-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGS 326
           P GW  W  D  +  L++AEYNNTG G+ T+ RV WPGYH+++ +T    FTVENF+ G 
Sbjct: 499 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVIN-STDANNFTVENFLLGD 558

Query: 327 QWLPETGIPFRTG 334
            W+ ++G+P+ +G
Sbjct: 559 GWMVQSGVPYISG 570

BLAST of CmoCh05G009280 vs. NCBI nr
Match: gi|778697621|ref|XP_011654361.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 508.4 bits (1308), Expect = 9.2e-141
Identity = 244/337 (72.40%), Postives = 288/337 (85.46%), Query Frame = 1

Query: 1   MPSFKLAMLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSI 60
           M  F L M+F++YLLV     FS C+SF+ TVSLDGT N++KI+DAIAAAPN ST RF I
Sbjct: 1   MTIFTLEMIFVLYLLVVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYI 60

Query: 61  HVKPGTYKELIEVPPSKTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQ 120
           HVKPGTY+E+IEVP  KT IALIGDD+STTIIV++RSNGTGS+TA+SATLTV G+NFMAQ
Sbjct: 61  HVKPGTYQEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQ 120

Query: 121 SLTFQNSAGPKEHQAVAVLDQAKHSAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFI 180
            LTFQN+AGP E QA+A+LDQA H+AYYKCVFLGYQDTLYAG LPQFF+ECDIYGS+DFI
Sbjct: 121 FLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFI 180

Query: 181 FGNGVTVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLL 240
           FGNG+ VFQDCNIY R F ++ TVTAQSK +++  SGF+FQNCKVTVSPEIASSKDKV +
Sbjct: 181 FGNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTV 240

Query: 241 YLGRPWKEYSTVVFIESFLDTVVQPKGWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWP 300
           +LGRPW+ YS VVFI+SFLD VVQPKGWL WP VP + L++AEYNN+G GA+TS+RV+WP
Sbjct: 241 FLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWP 300

Query: 301 GYHILHTATQVTRFTVENFIGGSQWLPETGIPFRTGF 335
           GYH L+ A +V  FTVE FI G+QWLP+TGIPFR GF
Sbjct: 301 GYHALNNAKEVATFTVEIFINGTQWLPQTGIPFRAGF 337

BLAST of CmoCh05G009280 vs. NCBI nr
Match: gi|778708551|ref|XP_011656233.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 508.4 bits (1308), Expect = 9.2e-141
Identity = 246/332 (74.10%), Postives = 283/332 (85.24%), Query Frame = 1

Query: 6   LAMLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPG 65
           L MLFIVYLLV     FS C+ F+ TVSLDGTGN+VKI+DAIAAAPN ST RF IHVKPG
Sbjct: 6   LKMLFIVYLLVVVIVEFSDCFIFNFTVSLDGTGNFVKINDAIAAAPNFSTTRFYIHVKPG 65

Query: 66  TYKELIEVPPSKTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQ 125
           TYKE+IEVP  KT IALIGDDASTTIIV++RSNGTGS+TA+SATLTV GANFMAQ LTFQ
Sbjct: 66  TYKEIIEVPYEKTCIALIGDDASTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQ 125

Query: 126 NSAGPKEHQAVAVLDQAKHSAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGV 185
           N+AGP E QA+A+LDQA H+AYYKCVFLGYQDTLYAG LPQFF+ECDIYGS+DFIFGNG+
Sbjct: 126 NTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGL 185

Query: 186 TVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRP 245
            VFQDCNIY R F ++ TVTAQSK +++  SGF+FQNC VTVSPEIA SKD V ++LGRP
Sbjct: 186 VVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRP 245

Query: 246 WKEYSTVVFIESFLDTVVQPKGWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHIL 305
           W+ YS VVFI+SFLD VVQPKGWL WP VP + L++AEYNN+G GA+TS+RV+WPGYH L
Sbjct: 246 WRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHAL 305

Query: 306 HTATQVTRFTVENFIGGSQWLPETGIPFRTGF 335
           + A +V  FTVE FI G+QWLP+TGIPFR GF
Sbjct: 306 NNAKEVATFTVETFINGTQWLPQTGIPFRAGF 337

BLAST of CmoCh05G009280 vs. NCBI nr
Match: gi|700198848|gb|KGN54006.1| (hypothetical protein Csa_4G260430 [Cucumis sativus])

HSP 1 Score: 504.6 bits (1298), Expect = 1.3e-139
Identity = 241/330 (73.03%), Postives = 285/330 (86.36%), Query Frame = 1

Query: 8   MLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTY 67
           M+F++YLLV     FS C+SF+ TVSLDGT N++KI+DAIAAAPN ST RF IHVKPGTY
Sbjct: 1   MIFVLYLLVVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTY 60

Query: 68  KELIEVPPSKTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNS 127
           +E+IEVP  KT IALIGDD+STTIIV++RSNGTGS+TA+SATLTV G+NFMAQ LTFQN+
Sbjct: 61  QEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNT 120

Query: 128 AGPKEHQAVAVLDQAKHSAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTV 187
           AGP E QA+A+LDQA H+AYYKCVFLGYQDTLYAG LPQFF+ECDIYGS+DFIFGNG+ V
Sbjct: 121 AGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVV 180

Query: 188 FQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWK 247
           FQDCNIY R F ++ TVTAQSK +++  SGF+FQNCKVTVSPEIASSKDKV ++LGRPW+
Sbjct: 181 FQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWR 240

Query: 248 EYSTVVFIESFLDTVVQPKGWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHT 307
            YS VVFI+SFLD VVQPKGWL WP VP + L++AEYNN+G GA+TS+RV+WPGYH L+ 
Sbjct: 241 MYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNN 300

Query: 308 ATQVTRFTVENFIGGSQWLPETGIPFRTGF 335
           A +V  FTVE FI G+QWLP+TGIPFR GF
Sbjct: 301 AKEVATFTVEIFINGTQWLPQTGIPFRAGF 330

BLAST of CmoCh05G009280 vs. NCBI nr
Match: gi|659131731|ref|XP_008465826.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 488.0 bits (1255), Expect = 1.3e-134
Identity = 228/306 (74.51%), Postives = 268/306 (87.58%), Query Frame = 1

Query: 29  VSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDDASTTI 88
           +SLDGTGN+VK++DAIAAAPN S  RF IHVKPGTY+E+IEVP  KTFIALIGDD+STTI
Sbjct: 19  LSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSSTTI 78

Query: 89  IVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSAYYKCV 148
           IV++RSNGTGS+TA+SATLTV G NFMAQ LTFQNSAGP E QA+AVLDQAKH+AYY+CV
Sbjct: 79  IVNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCV 138

Query: 149 FLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVREFGIKTTVTAQSKAN 208
           FLGYQDTLYAG +PQFF+ECDIYGS+DFIFGNG+ VFQDCNIY R F ++ TVTAQSK +
Sbjct: 139 FLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQS 198

Query: 209 IDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQPKGWLGW 268
           +   SGF+FQNC VTVSPEIASSKDKV ++LGRPW+ YS VVFI+SFLD+VVQPKGWL W
Sbjct: 199 LSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEW 258

Query: 269 PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGI 328
           P VP + L++AEYNN+G GA+TS+RV+WPGYH+L  A +V  F+VE FI G+QWLP+TGI
Sbjct: 259 PGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGI 318

Query: 329 PFRTGF 335
           PFR GF
Sbjct: 319 PFRAGF 324

BLAST of CmoCh05G009280 vs. NCBI nr
Match: gi|778657958|ref|XP_004152582.2| (PREDICTED: pectinesterase 2-like [Cucumis sativus])

HSP 1 Score: 410.6 bits (1054), Expect = 2.6e-111
Identity = 199/310 (64.19%), Postives = 241/310 (77.74%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVKPGTYKELIEVPPSKTFIALIGDD 83
           SF++TVSLDG+GN+ +ISDAIAAAPN S  RF IHV PGTY E +++P  K FIAL+GDD
Sbjct: 25  SFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDD 84

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAKHSA 143
             TTII+D+RSNGTG  T  SATLTV+G NFMA+SLTF+NSAGP+ +QAVAV D+A H+A
Sbjct: 85  PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTA 144

Query: 144 YYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVTVFQDCNIYVREFGIKTTVTA 203
           YYKC FL +QDTLY  G PQFF+E DIYGS+DFI G G  +FQDCNIY R      TVTA
Sbjct: 145 YYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTA 204

Query: 204 QSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDTVVQPK 263
           QSK  +   SGF FQNC VTVS EI+S+K  V ++LGRPWK+YS VVF+ESFLD VV  +
Sbjct: 205 QSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASE 264

Query: 264 GWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWL 323
           GW+ W  VPV+ LF+ E+NN GPGAD S+RV+W  YH+L   + + RFTV+NF+ GS+WL
Sbjct: 265 GWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESAL-RFTVDNFVNGSEWL 324

Query: 324 PETGIPFRTG 334
           PETGIPFR G
Sbjct: 325 PETGIPFRRG 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME39_ARATH3.7e-8146.18Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=P... [more]
PME2_CITSI3.7e-8146.13Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME44_ARATH2.2e-7845.22Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=P... [more]
PME32_ARATH2.9e-7844.87Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=P... [more]
PME20_ARATH2.5e-7745.37Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0L1M3_CUCSA9.2e-14073.03Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1[more]
A0A0A0LR14_CUCSA1.8e-11164.19Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1[more]
A0A151U4L2_CAJCA1.2e-8348.08Pectinesterase OS=Cajanus cajan GN=KK1_006907 PE=4 SV=1[more]
A0A0B0P3D3_GOSAR3.6e-8347.83Pectinesterase OS=Gossypium arboreum GN=F383_07269 PE=4 SV=1[more]
R9QQU4_GOSBA1.4e-8247.52Pectinesterase OS=Gossypium barbadense PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02300.12.1e-8246.18 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G33220.11.3e-7945.22 pectin methylesterase 44[more]
AT3G43270.11.6e-7944.87 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G47550.11.4e-7845.37 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02330.11.8e-7844.41 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|778697621|ref|XP_011654361.1|9.2e-14172.40PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|778708551|ref|XP_011656233.1|9.2e-14174.10PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|700198848|gb|KGN54006.1|1.3e-13973.03hypothetical protein Csa_4G260430 [Cucumis sativus][more]
gi|659131731|ref|XP_008465826.1|1.3e-13474.51PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
gi|778657958|ref|XP_004152582.2|2.6e-11164.19PREDICTED: pectinesterase 2-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
IPR018040Pectinesterase_Tyr_AS
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G009280.1CmoCh05G009280.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 26..319
score: 1.2E
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 21..332
score: 4.89
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 22..331
score: 9.1E
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 51..70
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 26..334
score: 1.0E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh05G009280CmoCh12G006530Cucurbita moschata (Rifu)cmocmoB155