CSPI05G16600 (gene) Wild cucumber (PI 183967)

NameCSPI05G16600
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionPectinesterase
LocationChr5 : 17922348 .. 17923010 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTGTTCTTGACCAAGCAAAACACACAGCTTACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCACTTCCCCAGTTCTTCAAAGAATGCGATATCTATGGAAGCGTCGACTTCATTTTTGGAAATGGACTTGTTGTATTCCAAGATTGTAATATATACGCTCGATTATTTGACGTTCAAATTACCGTAACAGCACAATCGAAACCAAGTTTAAATTCTCTGAGCGGCTTCATCTTCCAAAACTGCAATGTGACTGTGTCGCCAGAGATAGCATCAAGTAAGGACAACGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCAATGGTTGTTTTCATTGATTCATTTCTTGACAATGTGGTTCAACCTAAAGGATGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTATCATGTGTTAAATAACGCAAAAGAAGTTGCAACTTTTACTGTTGAAATATTTATTAATGGGACTCAATGGTTACCTCAAACTGGCATACCATTTAGAGCTGGTTTTTAG

mRNA sequence

ATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTGTTCTTGACCAAGCAAAACACACAGCTTACTACAAGTGTGTGTTTTTGGCACAATCGAAACCAAGTTTAAATTCTCTGAGCGGCTTCATCTTCCAAAACTGCAATGTGACTGTGTCGCCAGAGATAGCATCAAGTAAGGACAACGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCAATGGTTGTTTTCATTGATTCATTTCTTGACAATGTGGTTCAACCTAAAGGATGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTATCATGTGTTAAATAACGCAAAAGAAGTTGCAACTTTTACTGTTGAAATATTTATTAATGGGACTCAATGGTTACCTCAAACTGGCATACCATTTAGAGCTGGTTTTTAG

Coding sequence (CDS)

ATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTGTTCTTGACCAAGCAAAACACACAGCTTACTACAAGTGTGTGTTTTTGGCACAATCGAAACCAAGTTTAAATTCTCTGAGCGGCTTCATCTTCCAAAACTGCAATGTGACTGTGTCGCCAGAGATAGCATCAAGTAAGGACAACGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCAATGGTTGTTTTCATTGATTCATTTCTTGACAATGTGGTTCAACCTAAAGGATGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTATCATGTGTTAAATAACGCAAAAGAAGTTGCAACTTTTACTGTTGAAATATTTATTAATGGGACTCAATGGTTACCTCAAACTGGCATACCATTTAGAGCTGGTTTTTAG
BLAST of CSPI05G16600 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 8.5e-34
Identity = 62/132 (46.97%), Postives = 85/132 (64.39%), Query Frame = 1

Query: 37  AQSKPSLNSLSGFIFQNCNVTVSPEIASSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQP 96
           AQ +   N  +G I  NC VT + ++   + +V  FLGRPW+ YS  V+I +FLD+++ P
Sbjct: 378 AQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINP 437

Query: 97  KGWLEWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGYHVLNNAKEVATFTVEIFINGTQ 156
            GW+EW G    N LYYAEY N+G G++T+ RV W GYHVL +  +V+ FTV  FI G  
Sbjct: 438 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 497

Query: 157 WLPQTGIPFRAG 168
           WLP T +PF +G
Sbjct: 498 WLPATNVPFTSG 509

BLAST of CSPI05G16600 vs. Swiss-Prot
Match: PME25_ARATH (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=PME25 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.5e-33
Identity = 83/222 (37.39%), Postives = 113/222 (50.90%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYYKCVFLA-QSKPSLNSLSGF---------- 60
           MA  +TF+NTAGP + QA+A+ + A+ +++Y+C F   Q    ++SL  F          
Sbjct: 394 MAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTV 453

Query: 61  ---------IFQNCNVTVSPEIASSKDNVT------------------------------ 120
                    IFQNCN+     +A  K+ +T                              
Sbjct: 454 DFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEP 513

Query: 121 ----VFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPG-VPENLLYYAEYNNSGGGANTS 168
                FLGRPW+ YS  VF+ S++ ++VQP GWLEW G +  + +YY EY+N G GANT+
Sbjct: 514 KSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTN 573

BLAST of CSPI05G16600 vs. Swiss-Prot
Match: PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.0e-31
Identity = 73/222 (32.88%), Postives = 104/222 (46.85%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYYKCVFLA-QSKPSLNSLSGF---------- 60
           +A+ +TFQNTAGP + QA+A+        +Y+C     Q     +S+  F          
Sbjct: 303 IARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTV 362

Query: 61  ---------IFQNCNVTVSPEIASSKDNVT------------------------------ 120
                    +FQ+C +     + + K+++T                              
Sbjct: 363 DFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNL 422

Query: 121 ----VFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPG-VPENLLYYAEYNNSGGGANTS 168
                +LGRPW++YS  VF+ +++ + + P GWLEW G    + LYY EY NSG GA+  
Sbjct: 423 NTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLD 482

BLAST of CSPI05G16600 vs. Swiss-Prot
Match: PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=PME39 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 6.7e-31
Identity = 61/139 (43.88%), Postives = 81/139 (58.27%), Query Frame = 1

Query: 31  YKCVFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDNVTVFLGRPWRMYSMVVFIDSFL 90
           +K  F AQS+   +  +G    NC +  +P++   K+N   +LGRPWR YS  V I SF+
Sbjct: 394 HKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFI 453

Query: 91  DNVVQPKGWLEWP-GVPENLLYYAEYNNSGGGANTSQRVNWPGYHVLNNAKEVATFTVEI 150
           D+++ P GWLE         LYY EY N G GAN ++RV WPG+  + N  E   FTV  
Sbjct: 454 DDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGP 513

Query: 151 FINGTQWLPQTGIPFRAGF 169
           FI+G+ WL  TGIPF  GF
Sbjct: 514 FIDGSTWLNSTGIPFSLGF 532

BLAST of CSPI05G16600 vs. Swiss-Prot
Match: PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=PME41 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 8.8e-31
Identity = 59/132 (44.70%), Postives = 85/132 (64.39%), Query Frame = 1

Query: 37  AQSKPSLNSLSGFIFQNCNVTVSPEIASSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQP 96
           AQ +   N  +G    NC +  + ++ SS   V  +LGRPW+ YS  VF+ S++D VV+P
Sbjct: 440 AQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEP 499

Query: 97  KGWLEWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGYHVLNNAKEVATFTVEIFINGTQ 156
            GW EW G    + LYYAEYNN+G G++T+ RV WPGYHV+N+  +   FTVE F+ G  
Sbjct: 500 VGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINST-DANNFTVENFLLGDG 559

Query: 157 WLPQTGIPFRAG 168
           W+ Q+G+P+ +G
Sbjct: 560 WMVQSGVPYISG 570

BLAST of CSPI05G16600 vs. TrEMBL
Match: A0A0A0KQN0_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G488750 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 1.5e-93
Identity = 163/168 (97.02%), Postives = 164/168 (97.62%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYYKCVFLAQSKPSLNSLSGFIFQNCNVTVSP 60
           MAQFLTFQNTAGPNEGQAIA+LDQA HTAYYKCVFLAQSKPSLNSLSGFIFQNCNVTVSP
Sbjct: 1   MAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLAQSKPSLNSLSGFIFQNCNVTVSP 60

Query: 61  EIASSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGG 120
           EIA SKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGG
Sbjct: 61  EIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGG 120

Query: 121 GANTSQRVNWPGYHVLNNAKEVATFTVEIFINGTQWLPQTGIPFRAGF 169
           GANTSQRVNWPGYH LNNAKEVATFTVE FINGTQWLPQTGIPFRAGF
Sbjct: 121 GANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGIPFRAGF 168

BLAST of CSPI05G16600 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 1.5e-82
Identity = 158/220 (71.82%), Postives = 160/220 (72.73%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYY----------------------------- 60
           MAQFLTFQNTAGPNEGQAIA+LDQA HTAYY                             
Sbjct: 111 MAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSV 170

Query: 61  -----------------------KCVFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDN 120
                                  +    AQSKPSLNSLSGFIFQNC VTVSPEIASSKD 
Sbjct: 171 DFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDK 230

Query: 121 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 169
           VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV
Sbjct: 231 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 290

BLAST of CSPI05G16600 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 9.4e-48
Identity = 101/219 (46.12%), Postives = 127/219 (57.99%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYY----------------------------- 60
           MA+ LTF+N+AGP   QA+AV D+A HTAYY                             
Sbjct: 116 MAESLTFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSV 175

Query: 61  -----------------------KCVFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDN 120
                                       AQSK  L S+SGF FQNC VTVS EI+S+K N
Sbjct: 176 DFICGYGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQN 235

Query: 121 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 168
           V VFLGRPW+ YS VVF++SFLD+VV  +GW+EW GVP N L+Y E+NN G GA+ S+RV
Sbjct: 236 VKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRV 295

BLAST of CSPI05G16600 vs. TrEMBL
Match: W9R0V2_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_012494 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.4e-38
Identity = 87/219 (39.73%), Postives = 119/219 (54.34%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYY----------------------------- 60
           +AQ LT +N+AGP   QA A+ + A  +A+Y                             
Sbjct: 6   VAQSLTIENSAGPQNHQAAALYNTADQSAFYNCIFLGYQDTLYAKENRQFYKDCKIYGTV 65

Query: 61  -------KCVF---------------LAQSKPSLNSLSGFIFQNCNVTVSPEIAS-SKDN 120
                  K VF                AQ++ S + ++GF FQ C+V  +P +    + N
Sbjct: 66  DFIFGHAKAVFQDCHIYARPETLITITAQNRASDSEVNGFSFQGCHVEATPGLTPPQRAN 125

Query: 121 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 168
           + VFLGRPW  YS V+F+ S  D++V P+GW EWPGVP + LYYAEY+NSG GA+TS+RV
Sbjct: 126 LRVFLGRPWDRYSTVIFMQSIFDDIVDPQGWTEWPGVPVDHLYYAEYDNSGLGADTSKRV 185

BLAST of CSPI05G16600 vs. TrEMBL
Match: A0A162AH30_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_001692 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.6e-37
Identity = 84/217 (38.71%), Postives = 115/217 (53.00%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYY----------------------------- 60
           +AQ +TF+NTAGP+ GQ IA+  Q+  +A+Y                             
Sbjct: 293 VAQDITFENTAGPSAGQEIALFSQSDQSAFYRCRFKGYQDTLLASMNRQFYRECEIYGTV 352

Query: 61  ------------KC------VFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDNVTVFL 120
                       KC      V  AQ +   NS +G + QNC +   PE+ +       +L
Sbjct: 353 DYIFGNAAVVFQKCLIYAGTVTTAQGRTDPNSPTGTVIQNCRIVSGPELPAGPS--PAYL 412

Query: 121 GRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPE---NLLYYAEYNNSGGGANTSQRVNW 168
           GRPW  YS+VV + SFLDNVV P GWLEWPG  +   + LYYAE+NN+G G+ T+ RV W
Sbjct: 413 GRPWFDYSLVVVMQSFLDNVVNPAGWLEWPGAGQGRYSTLYYAEFNNNGPGSGTNARVKW 472

BLAST of CSPI05G16600 vs. TAIR10
Match: AT3G10720.2 (AT3G10720.2 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 143.3 bits (360), Expect = 1.4e-34
Identity = 83/222 (37.39%), Postives = 113/222 (50.90%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYYKCVFLA-QSKPSLNSLSGF---------- 60
           MA  +TF+NTAGP + QA+A+ + A+ +++Y+C F   Q    ++SL  F          
Sbjct: 394 MAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTV 453

Query: 61  ---------IFQNCNVTVSPEIASSKDNVT------------------------------ 120
                    IFQNCN+     +A  K+ +T                              
Sbjct: 454 DFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEP 513

Query: 121 ----VFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPG-VPENLLYYAEYNNSGGGANTS 168
                FLGRPW+ YS  VF+ S++ ++VQP GWLEW G +  + +YY EY+N G GANT+
Sbjct: 514 KSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTN 573

BLAST of CSPI05G16600 vs. TAIR10
Match: AT3G43270.1 (AT3G43270.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 136.3 bits (342), Expect = 1.7e-32
Identity = 73/222 (32.88%), Postives = 104/222 (46.85%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYYKCVFLA-QSKPSLNSLSGF---------- 60
           +A+ +TFQNTAGP + QA+A+        +Y+C     Q     +S+  F          
Sbjct: 303 IARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTV 362

Query: 61  ---------IFQNCNVTVSPEIASSKDNVT------------------------------ 120
                    +FQ+C +     + + K+++T                              
Sbjct: 363 DFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNL 422

Query: 121 ----VFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPG-VPENLLYYAEYNNSGGGANTS 168
                +LGRPW++YS  VF+ +++ + + P GWLEW G    + LYY EY NSG GA+  
Sbjct: 423 NTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLD 482

BLAST of CSPI05G16600 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 135.2 bits (339), Expect = 3.8e-32
Identity = 61/139 (43.88%), Postives = 81/139 (58.27%), Query Frame = 1

Query: 31  YKCVFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDNVTVFLGRPWRMYSMVVFIDSFL 90
           +K  F AQS+   +  +G    NC +  +P++   K+N   +LGRPWR YS  V I SF+
Sbjct: 394 HKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFI 453

Query: 91  DNVVQPKGWLEWP-GVPENLLYYAEYNNSGGGANTSQRVNWPGYHVLNNAKEVATFTVEI 150
           D+++ P GWLE         LYY EY N G GAN ++RV WPG+  + N  E   FTV  
Sbjct: 454 DDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGP 513

Query: 151 FINGTQWLPQTGIPFRAGF 169
           FI+G+ WL  TGIPF  GF
Sbjct: 514 FIDGSTWLNSTGIPFSLGF 532

BLAST of CSPI05G16600 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 134.8 bits (338), Expect = 4.9e-32
Identity = 59/132 (44.70%), Postives = 85/132 (64.39%), Query Frame = 1

Query: 37  AQSKPSLNSLSGFIFQNCNVTVSPEIASSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQP 96
           AQ +   N  +G    NC +  + ++ SS   V  +LGRPW+ YS  VF+ S++D VV+P
Sbjct: 440 AQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEP 499

Query: 97  KGWLEWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGYHVLNNAKEVATFTVEIFINGTQ 156
            GW EW G    + LYYAEYNN+G G++T+ RV WPGYHV+N+  +   FTVE F+ G  
Sbjct: 500 VGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINST-DANNFTVENFLLGDG 559

Query: 157 WLPQTGIPFRAG 168
           W+ Q+G+P+ +G
Sbjct: 560 WMVQSGVPYISG 570

BLAST of CSPI05G16600 vs. TAIR10
Match: AT1G23200.1 (AT1G23200.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 134.8 bits (338), Expect = 4.9e-32
Identity = 73/213 (34.27%), Postives = 104/213 (48.83%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYYKC--------VFLAQSKPSLNSLS----- 60
           +AQ +TF+NTAGP + QA+A+   +  + +Y C        ++L  S+  L + +     
Sbjct: 341 IAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTV 400

Query: 61  GFIF-------QNCNVTVSPEIASSKDNVT-------------------------VFLGR 120
            FIF       QNCN+     ++  K+ +T                          +LGR
Sbjct: 401 DFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASETYLGR 460

Query: 121 PWRMYSMVVFIDSFLDNVVQPKGWLEWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGYH 168
           PWR +S  VF+   L  +V P GWL W G    + LYY EY N+G GA+ S RV WPGYH
Sbjct: 461 PWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYH 520

BLAST of CSPI05G16600 vs. NCBI nr
Match: gi|700196022|gb|KGN51199.1| (hypothetical protein Csa_5G488750 [Cucumis sativus])

HSP 1 Score: 350.1 bits (897), Expect = 2.1e-93
Identity = 163/168 (97.02%), Postives = 164/168 (97.62%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYYKCVFLAQSKPSLNSLSGFIFQNCNVTVSP 60
           MAQFLTFQNTAGPNEGQAIA+LDQA HTAYYKCVFLAQSKPSLNSLSGFIFQNCNVTVSP
Sbjct: 1   MAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLAQSKPSLNSLSGFIFQNCNVTVSP 60

Query: 61  EIASSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGG 120
           EIA SKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGG
Sbjct: 61  EIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGG 120

Query: 121 GANTSQRVNWPGYHVLNNAKEVATFTVEIFINGTQWLPQTGIPFRAGF 169
           GANTSQRVNWPGYH LNNAKEVATFTVE FINGTQWLPQTGIPFRAGF
Sbjct: 121 GANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGIPFRAGF 168

BLAST of CSPI05G16600 vs. NCBI nr
Match: gi|778708551|ref|XP_011656233.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 314.3 bits (804), Expect = 1.3e-82
Identity = 158/220 (71.82%), Postives = 160/220 (72.73%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYY----------------------------- 60
           MAQFLTFQNTAGPNEGQAIA+LDQA HTAYY                             
Sbjct: 118 MAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSV 177

Query: 61  -----------------------KCVFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDN 120
                                  +    AQSKPSLNSLSGFIFQNCNVTVSPEIA SKDN
Sbjct: 178 DFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCNVTVSPEIAPSKDN 237

Query: 121 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 169
           VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV
Sbjct: 238 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 297

BLAST of CSPI05G16600 vs. NCBI nr
Match: gi|700198848|gb|KGN54006.1| (hypothetical protein Csa_4G260430 [Cucumis sativus])

HSP 1 Score: 313.5 bits (802), Expect = 2.2e-82
Identity = 158/220 (71.82%), Postives = 160/220 (72.73%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYY----------------------------- 60
           MAQFLTFQNTAGPNEGQAIA+LDQA HTAYY                             
Sbjct: 111 MAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSV 170

Query: 61  -----------------------KCVFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDN 120
                                  +    AQSKPSLNSLSGFIFQNC VTVSPEIASSKD 
Sbjct: 171 DFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDK 230

Query: 121 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 169
           VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV
Sbjct: 231 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 290

BLAST of CSPI05G16600 vs. NCBI nr
Match: gi|778697621|ref|XP_011654361.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 313.5 bits (802), Expect = 2.2e-82
Identity = 158/220 (71.82%), Postives = 160/220 (72.73%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYY----------------------------- 60
           MAQFLTFQNTAGPNEGQAIA+LDQA HTAYY                             
Sbjct: 118 MAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSV 177

Query: 61  -----------------------KCVFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDN 120
                                  +    AQSKPSLNSLSGFIFQNC VTVSPEIASSKD 
Sbjct: 178 DFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDK 237

Query: 121 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 169
           VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV
Sbjct: 238 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 297

BLAST of CSPI05G16600 vs. NCBI nr
Match: gi|659131731|ref|XP_008465826.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 301.6 bits (771), Expect = 8.6e-79
Identity = 151/220 (68.64%), Postives = 161/220 (73.18%), Query Frame = 1

Query: 1   MAQFLTFQNTAGPNEGQAIAVLDQAKHTAYY----------------------------- 60
           MAQFLTFQN+AGPNEGQAIAVLDQAKHTAYY                             
Sbjct: 105 MAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCVFLGYQDTLYAGSVPQFFKECDIYGSV 164

Query: 61  -----------------------KCVFLAQSKPSLNSLSGFIFQNCNVTVSPEIASSKDN 120
                                  +    AQSK SL++ SGFIFQNCNVTVSPEIASSKD 
Sbjct: 165 DFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQSLSAPSGFIFQNCNVTVSPEIASSKDK 224

Query: 121 VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRV 169
           VTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWLEWPGVPEN+LYYAEYNNSGGGANTSQRV
Sbjct: 225 VTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEWPGVPENVLYYAEYNNSGGGANTSQRV 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME2_CITSI8.5e-3446.97Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME25_ARATH2.5e-3337.39Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=P... [more]
PME32_ARATH3.0e-3132.88Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=P... [more]
PME39_ARATH6.7e-3143.88Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=P... [more]
PME41_ARATH8.8e-3144.70Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0KQN0_CUCSA1.5e-9397.02Pectinesterase OS=Cucumis sativus GN=Csa_5G488750 PE=4 SV=1[more]
A0A0A0L1M3_CUCSA1.5e-8271.82Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1[more]
A0A0A0LR14_CUCSA9.4e-4846.12Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1[more]
W9R0V2_9ROSA1.4e-3839.73Pectinesterase OS=Morus notabilis GN=L484_012494 PE=4 SV=1[more]
A0A162AH30_DAUCA2.6e-3738.71Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_001692 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10720.21.4e-3437.39 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G43270.11.7e-3232.88 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02300.13.8e-3243.88 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02330.14.9e-3244.70 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G23200.14.9e-3234.27 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|700196022|gb|KGN51199.1|2.1e-9397.02hypothetical protein Csa_5G488750 [Cucumis sativus][more]
gi|778708551|ref|XP_011656233.1|1.3e-8271.82PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|700198848|gb|KGN54006.1|2.2e-8271.82hypothetical protein Csa_4G260430 [Cucumis sativus][more]
gi|778697621|ref|XP_011654361.1|2.2e-8271.82PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|659131731|ref|XP_008465826.1|8.6e-7968.64PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G16600.1CSPI05G16600.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 37..153
score: 7.1
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 4..166
score: 1.05
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 37..165
score: 3.8E-39coord: 2..36
score: 3.
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 1..168
score: 4.4