Lsi02G017320 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi02G017320
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPectinesterase
Locationchr02 : 23002588 .. 23003916 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTAGATGGTACAGGAAATTTTGTTAAAGTTAATGATGCTATAGCAGCTGCACCAAATTTCAGTACTACAAGATTTTACATTCATGTAAAACCTGGAACTTATAATGAAATTATTGAAGTTCCATCTCAAAAAACTTTCATTGCTTTAATTGGAGATGATGCTTCTACCACCATTATTGTTAACAATCGAAGCAATGCCACTGGATTCTCCACAGCAACTACTGCTACTCTCAGTAAGCTTCAATCCCGACTTGTTTGGTAATCATTTGATTTTTTATTTTTGATTTTTTAAAATCAAACCAATAATAAACAATGCTTCCACTTCTAAATTATTTTTTTTATTACCTATTTTTTACATCTCTATTGGGATGAGATATAATAAGGACATTACCGGTGTGTTAATCCTAATTGAGATATTTGGGTGCGTCTACTGATCCCTTTACATTTAGTTTCCCGTTAAAAAAAATTACCCTACGTTTTATCAATCTTTTTAAAAACTAAGGCATGTTTTTAAAAATTTGGTACTTTAAAAAAATGTAAAACACGGTAAGAAGTTGAGAGAAAATAAGTTTGATTTTTAAAAACCAAATGGTTATGAAATCGGGTCTAAATTTTTTAATTACTAAAACAATGTTTGATTTTGGTAGATGTAAATGGAGGAAACTTCATGGCTCAGTCCTTGACCTTTGAAAACTCTGCTGGACCAAATGGGGGTCAAGCAATTGCTGTTCTTGACAAAGCAACACACACAGCATACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCAGTTCCCCAATTCTTCAAAGAATGCAATATATATGGAAGCATCGACTTTATTTTCGGAAACGGGCTTGTTATATTCCAAGACTGTAATATATACACTCGATTACCCGGTGTTGAGAGTACTGTAACGGCACAATCCAAAGAGACTTTAGATGCTATGAGCGGCTTCGTCTTCCAAAACTGCACTGTGACAGTGTCGCCGGAGATAGCTTCGAGTAAGGACAAGGTAACGGTGTTTCTTGGACGGCCATGGAGAATTTATTCGATGGTTACTTTCATTAAGTCATTTCTTGACAATGTGGTTCAACCTAAAGGATGGTTGGAGTGGGATGGAGTACCAGAGAATCTATTGTTTTATGCAGAATACAATAATAGAGGTGCAGGAGCTGACACTTCCCAAAGGGTGAATTGGCCTGGTTTCCATGTATTACACAATGCACACGAAGTTGCAACTTTTACCGTTGAAATTTTTATTCATGGGACTCATTGGTTGCCTGAAACTGGCATACCATTTAGGCCTGCTTTT

mRNA sequence

TCTTTAGATGGTACAGGAAATTTTGTTAAAGTTAATGATGCTATAGCAGCTGCACCAAATTTCAGTACTACAAGATTTTACATTCATGTAAAACCTGGAACTTATAATGAAATTATTGAAGTTCCATCTCAAAAAACTTTCATTGCTTTAATTGGAGATGATGCTTCTACCACCATTATTTCCTTGACCTTTGAAAACTCTGCTGGACCAAATGGGGGTCAAGCAATTGCTGTTCTTGACAAAGCAACACACACAGCATACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCAGTTCCCCAATTCTTCAAAGAATGCAATATATATGGAAGCATCGACTTTATTTTCGGAAACGGGCTTGTTATATTCCAAGACTGTAATATATACACTCGATTACCCGGTGTTGAGAGTACTGTAACGGCACAATCCAAAGAGACTTTAGATGCTATGAGCGGCTTCGTCTTCCAAAACTGCACTGTGACAGTGTCGCCGGAGATAGCTTCGAGTAAGGACAAGGTAACGGTGTTTCTTGGACGGCCATGGAGAATTTATTCGATGGTTACTTTCATTAAGTCATTTCTTGACAATGTGGTTCAACCTAAAGGATGGTTGGAGTGGGATGGAGTACCAGAGAATCTATTGTTTTATGCAGAATACAATAATAGAGGTGCAGGAGCTGACACTTCCCAAAGGGTGAATTGGCCTGGTTTCCATGTATTACACAATGCACACGAAGTTGCAACTTTTACCGTTGAAATTTTTATTCATGGGACTCATTGGTTGCCTGAAACTGGCATACCATTTAGGCCTGCTTTT

Coding sequence (CDS)

TCTTTAGATGGTACAGGAAATTTTGTTAAAGTTAATGATGCTATAGCAGCTGCACCAAATTTCAGTACTACAAGATTTTACATTCATGTAAAACCTGGAACTTATAATGAAATTATTGAAGTTCCATCTCAAAAAACTTTCATTGCTTTAATTGGAGATGATGCTTCTACCACCATTATTTCCTTGACCTTTGAAAACTCTGCTGGACCAAATGGGGGTCAAGCAATTGCTGTTCTTGACAAAGCAACACACACAGCATACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCAGTTCCCCAATTCTTCAAAGAATGCAATATATATGGAAGCATCGACTTTATTTTCGGAAACGGGCTTGTTATATTCCAAGACTGTAATATATACACTCGATTACCCGGTGTTGAGAGTACTGTAACGGCACAATCCAAAGAGACTTTAGATGCTATGAGCGGCTTCGTCTTCCAAAACTGCACTGTGACAGTGTCGCCGGAGATAGCTTCGAGTAAGGACAAGGTAACGGTGTTTCTTGGACGGCCATGGAGAATTTATTCGATGGTTACTTTCATTAAGTCATTTCTTGACAATGTGGTTCAACCTAAAGGATGGTTGGAGTGGGATGGAGTACCAGAGAATCTATTGTTTTATGCAGAATACAATAATAGAGGTGCAGGAGCTGACACTTCCCAAAGGGTGAATTGGCCTGGTTTCCATGTATTACACAATGCACACGAAGTTGCAACTTTTACCGTTGAAATTTTTATTCATGGGACTCATTGGTTGCCTGAAACTGGCATACCATTTAGGCCTGCTTTT

Protein sequence

SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTIISLTFENSAGPNGGQAIAVLDKATHTAYYKCVFLGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPGVESTVTAQSKETLDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWDGVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGIPFRPAF
BLAST of Lsi02G017320 vs. Swiss-Prot
Match: PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=PME39 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 9.2e-70
Identity = 132/308 (42.86%), Postives = 177/308 (57.47%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           ++DGTGNF  +NDA+ AAPN S TRF I++K G Y E +E+P +KT I  IGD    T+I
Sbjct: 225 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 284

Query: 61  S----------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVF 120
                                        ++F NSAGP   QA+A    + H+A+Y+C F
Sbjct: 285 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 344

Query: 121 LGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRL--PGVESTVTAQSKE 180
            GYQDTLY  +  QF++EC+IYG+IDFIFGN  V+FQ+ ++Y R   PG +   TAQS+ 
Sbjct: 345 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 404

Query: 181 TLDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLE 240
             D  +G    NC +  +P++   K+    +LGRPWR YS    IKSF+D+++ P GWLE
Sbjct: 405 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 464

Query: 241 W-DGVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPET 278
                    L+Y EY N G GA+ ++RV WPGF  + N  E   FTV  FI G+ WL  T
Sbjct: 465 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 524

BLAST of Lsi02G017320 vs. Swiss-Prot
Match: PME44_ARATH (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=PME44 PE=2 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 4.7e-66
Identity = 119/304 (39.14%), Postives = 181/304 (59.54%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           +LDGTGNF K+ DAI  AP++S+TRF I++K G Y E +E+  +K  I ++GD    T+I
Sbjct: 216 ALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVI 275

Query: 61  S----------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVF 120
           S                            +TF+N+AGP   QA+A+   +  + +++C  
Sbjct: 276 SGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSVFFRCAM 335

Query: 121 LGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTR--LPGVESTVTAQSKE 180
            GYQDTLY   + QF++EC I G++DFIFG+G V+FQ+C I  +  LP  ++T+TAQ ++
Sbjct: 336 RGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTITAQGRK 395

Query: 181 TLDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLE 240
            ++  SGF  Q   ++   ++    +    +LGRPW++YS   FI++ + +VV+P+GWLE
Sbjct: 396 DVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLE 455

Query: 241 WDG-VPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPET 274
           W+     + LFY E+ N G G+  S RV WPG+HV +N+ +   FTV  FI G  WLP T
Sbjct: 456 WNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGNLWLPST 515

BLAST of Lsi02G017320 vs. Swiss-Prot
Match: PME12_ARATH (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana GN=PME12 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 8.1e-66
Identity = 125/302 (41.39%), Postives = 177/302 (58.61%), Query Frame = 1

Query: 3   DGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTIIS- 62
           DGTGNF  +N+AI+ APN S  R  I+VK G Y+E I++P  KT I LIGD +  T I+ 
Sbjct: 243 DGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTFITG 302

Query: 63  ---------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVFLG 122
                                      +   N+AGP   QA+A+   A   A Y+CV  G
Sbjct: 303 NRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCVIDG 362

Query: 123 YQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRL--PGVESTVTAQSKETL 182
           YQDTLY  +  QF++EC+IYG+ID+IFGN  V+FQ CNI ++L  PG  + +TAQS++T 
Sbjct: 363 YQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQSRDTQ 422

Query: 183 DAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWD 242
           D  +G   QNC++  S ++ +S +KV  +LGRPWR +S    ++S++D  +   GW +W+
Sbjct: 423 DEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGWSKWN 482

Query: 243 GVPE-NLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 274
           G    + L+Y EYNN G G++T +RVNWPGFH++    +   FT   FI G  WL  T  
Sbjct: 483 GGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGDGWLGSTSF 542

BLAST of Lsi02G017320 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.3e-65
Identity = 123/301 (40.86%), Postives = 176/301 (58.47%), Query Frame = 1

Query: 3   DGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTIIS- 62
           DG+GN   + +A+AAA     +R+ I++K GTYNE IEV  +   I  +GD    TII+ 
Sbjct: 208 DGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIGKTIITG 267

Query: 63  ---------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVFLG 122
                                      +T  N+AGPN  QA+A+   +  + +Y+C F G
Sbjct: 268 SKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEG 327

Query: 123 YQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRL-PGVESTVTAQSKETLD 182
           YQDTLY  +  QF++EC+IYG++DFIFGN  V+ Q+CNI+ R  P   +T+TAQ +   +
Sbjct: 328 YQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQGRTDPN 387

Query: 183 AMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWDG 242
             +G +  NC VT + ++   +  V  FLGRPW+ YS   +IK+FLD+++ P GW+EW G
Sbjct: 388 QSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSG 447

Query: 243 -VPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGIP 274
               N L+YAEY N G G+ T+ RV W G+HVL +  +V+ FTV  FI G  WLP T +P
Sbjct: 448 DFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVP 506

BLAST of Lsi02G017320 vs. Swiss-Prot
Match: PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 5.2e-65
Identity = 120/307 (39.09%), Postives = 178/307 (57.98%), Query Frame = 1

Query: 3   DGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTIIS- 62
           DGTGNF  V DA+ AAP++S  R+ I++K GTY E +E+  +K  + +IGD    TIIS 
Sbjct: 214 DGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISG 273

Query: 63  ---------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVFLG 122
                                      +TFEN+AGP   QA+A+   +  + +Y+C   G
Sbjct: 274 NRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRG 333

Query: 123 YQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTR--LPGVESTVTAQSKETL 182
           YQDTLY   + QF+++C I G++DFIFG+  V+FQ+C I  +  LP  ++++TAQ ++  
Sbjct: 334 YQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDP 393

Query: 183 DAMSGFVFQNCTVTVSPEI-ASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEW 242
           +  +G   Q C +T   ++ A+S +    +LGRPW++YS    ++SFL NV++P+GWLEW
Sbjct: 394 NEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEW 453

Query: 243 DG-VPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETG 278
           +G    N LFY EY N G GA    RV WPG+ V + + +   +TV  FI G  WLP TG
Sbjct: 454 NGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTG 513

BLAST of Lsi02G017320 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.0e-127
Identity = 228/306 (74.51%), Postives = 253/306 (82.68%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           SLDGT NF+K+NDAIAAAPNFSTTRFYIHVKPGTY EIIEVP +KT IALIGDD+STTII
Sbjct: 26  SLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTII 85

Query: 61  ----------------------------SLTFENSAGPNG-GQAIAVLDKATHTAYYKCV 120
                                        LTF+N+AG    GQAIA+LD+ATHTAYYKCV
Sbjct: 86  VNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAG-PNEGQAIALLDQATHTAYYKCV 145

Query: 121 FLGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPGVESTVTAQSKET 180
           FLGYQDTLYAGA+PQFFKEC+IYGS+DFIFGNGLV+FQDCNIY RL  V+ TVTAQSK +
Sbjct: 146 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 205

Query: 181 LDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEW 240
           L+++SGF+FQNC VTVSPEIASSKDKVTVFLGRPWR+YSMV FI SFLDNVVQPKGWLEW
Sbjct: 206 LNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 265

Query: 241 DGVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 278
            GVPENLL+YAEYNN G GA+TSQRVNWPG+H L+NA EVATFTVEIFI+GT WLP+TGI
Sbjct: 266 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVEIFINGTQWLPQTGI 325

BLAST of Lsi02G017320 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.6e-97
Identity = 179/302 (59.27%), Postives = 213/302 (70.53%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           SLDG+GNF +++DAIAAAPN S TRFYIHV PGTY+E +++P  K FIAL+GDD  TTII
Sbjct: 31  SLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDDPFTTII 90

Query: 61  ----------------------------SLTFENSAGPNGGQAIAVLDKATHTAYYKCVF 120
                                       SLTFENSAGP   QA+AV DKA HTAYYKC F
Sbjct: 91  IDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKCRF 150

Query: 121 LGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPGVESTVTAQSKETL 180
           L +QDTLY    PQFFKE +IYGS+DFI G G V+FQDCNIY R+P    TVTAQSK  L
Sbjct: 151 LSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTAQSKYIL 210

Query: 181 DAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWD 240
            ++SGF FQNCTVTVS EI+S+K  V VFLGRPW+ YS V F++SFLD+VV  +GW+EW 
Sbjct: 211 RSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEWI 270

Query: 241 GVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGIP 275
           GVP N LFY E+NN G GAD S+RVNW  +H+L +      FTV+ F++G+ WLPETGIP
Sbjct: 271 GVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLL-DKESALRFTVDNFVNGSEWLPETGIP 330

BLAST of Lsi02G017320 vs. TrEMBL
Match: A0A072U3V6_MEDTR (Pectinesterase OS=Medicago truncatula GN=MTR_7g108905 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.6e-71
Identity = 131/302 (43.38%), Postives = 184/302 (60.93%), Query Frame = 1

Query: 3   DGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII-- 62
           DGTGNF  + +AIAAAPN S TRF IH+K G Y E +EV  +KT + L+GD    T++  
Sbjct: 251 DGTGNFATIGEAIAAAPNSSATRFVIHIKAGAYFENVEVIKKKTNLMLVGDGIGQTVVKA 310

Query: 63  --------------------------SLTFENSAGPNGGQAIAVLDKATHTAYYKCVFLG 122
                                      +TFENSAGP+  QA+AV + A  +A+Y+C F+ 
Sbjct: 311 SRNVVDGWTTFQSSTFAVVGDKFIAKGITFENSAGPSKHQAVAVRNGADFSAFYQCSFVA 370

Query: 123 YQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPG--VESTVTAQSKETL 182
           YQDTLY  ++ QF++EC++YG++DFIFGN  V+FQ+CN+Y R P    ++  TAQ +E  
Sbjct: 371 YQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYARKPDPKQKNLFTAQGREDP 430

Query: 183 DAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWD 242
           +  +G    NC +  + ++   +     +LGRPW+ YS   ++ SF+DN++ P GWLEW+
Sbjct: 431 NQNTGISILNCKIAAAADLIPVQSTFKSYLGRPWKKYSRTVYLNSFIDNLIDPAGWLEWN 490

Query: 243 GV-PENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 274
           G    + LFY EY NRG G++TS RV WPG+ V+ NA E + FTV  FI G  WL  TG+
Sbjct: 491 GTFALDTLFYGEYKNRGPGSNTSARVTWPGYKVITNATEASQFTVRQFIQGNEWLNSTGV 550

BLAST of Lsi02G017320 vs. TrEMBL
Match: A0A151U4L2_CAJCA (Pectinesterase OS=Cajanus cajan GN=KK1_006907 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 6.4e-70
Identity = 129/302 (42.72%), Postives = 185/302 (61.26%), Query Frame = 1

Query: 3   DGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII-- 62
           DGTGNF  + +A+A APN STTRF IH+K G Y E +EV  +KT +  +GD    T++  
Sbjct: 164 DGTGNFTTIGEALAVAPNSSTTRFVIHIKAGAYLENVEVIRKKTNLMFVGDGIGKTVVKG 223

Query: 63  --------------------------SLTFENSAGPNGGQAIAVLDKATHTAYYKCVFLG 122
                                      +TFENSAGP   QA+A+   A  +A+Y+C F+G
Sbjct: 224 SRNVVDGWTTFQSATVAVVGAGFIAKGITFENSAGPEKHQAVALRSGADFSAFYQCSFVG 283

Query: 123 YQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLP--GVESTVTAQSKETL 182
           YQDTLY  ++ QF++EC+IYG++DFIFGN  V+FQ+CN+Y R P    ++  TAQ +E  
Sbjct: 284 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYARKPNQNQKNLFTAQGREDP 343

Query: 183 DAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEW- 242
           +  +G    NC V  + ++   K     +LGRPW++YS   F+KS+++++++P GWLEW 
Sbjct: 344 NQNTGISILNCKVAAAADLIPVKTSFKSYLGRPWKMYSRTVFLKSYMEDLIEPAGWLEWN 403

Query: 243 DGVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 274
           D    + L+Y EY NRG G++TS RV WPG+ V++++ E + FTV  FI G  WL  TGI
Sbjct: 404 DTFALDTLYYGEYMNRGPGSNTSGRVTWPGYRVINSSTEASQFTVGEFIQGNDWLNSTGI 463

BLAST of Lsi02G017320 vs. TrEMBL
Match: A0A0D2SCA1_GOSRA (Pectinesterase OS=Gossypium raimondii GN=B456_007G127000 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 7.1e-69
Identity = 126/302 (41.72%), Postives = 182/302 (60.26%), Query Frame = 1

Query: 3   DGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII-- 62
           DGTGNF  +NDA+AAAPN S TRF I++K G+Y E +EV  +K  +  +GD    T++  
Sbjct: 254 DGTGNFTTINDAVAAAPNNSNTRFVIYIKAGSYLENVEVVKKKKMLMFVGDGIGKTVVKA 313

Query: 63  --------------------------SLTFENSAGPNGGQAIAVLDKATHTAYYKCVFLG 122
                                      +TFENSAGP+  QA+A+   +  +A+YKC F+ 
Sbjct: 314 SRNVVDGFTTFRSATVAVVGEGFIAKGITFENSAGPSKHQAVALRSGSDLSAFYKCSFVA 373

Query: 123 YQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRL--PGVESTVTAQSKETL 182
           YQDTLY  ++ QF++EC+IYG++DFIFGN  V+FQDCN+Y R   P  ++  TAQ +E  
Sbjct: 374 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYARKPNPNQKNIFTAQGREDP 433

Query: 183 DAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWD 242
           +  +G    NC +  + ++   K     +LGRPW+ YS    ++S++D+ V P GWLEW+
Sbjct: 434 NQNTGISILNCKIAAAADLVPVKSSFKTYLGRPWKEYSRTVIMRSYIDDSVDPAGWLEWN 493

Query: 243 GV-PENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 274
           G    + L+Y EY NRG G++TS RV WPG+ V++++ E   FT+E FI G  WL  + I
Sbjct: 494 GTFALSTLYYGEYLNRGPGSNTSARVTWPGYRVINSSTEATQFTIEPFIQGNEWLNSSNI 553

BLAST of Lsi02G017320 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 265.0 bits (676), Expect = 5.2e-71
Identity = 132/308 (42.86%), Postives = 177/308 (57.47%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           ++DGTGNF  +NDA+ AAPN S TRF I++K G Y E +E+P +KT I  IGD    T+I
Sbjct: 225 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 284

Query: 61  S----------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVF 120
                                        ++F NSAGP   QA+A    + H+A+Y+C F
Sbjct: 285 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 344

Query: 121 LGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRL--PGVESTVTAQSKE 180
            GYQDTLY  +  QF++EC+IYG+IDFIFGN  V+FQ+ ++Y R   PG +   TAQS+ 
Sbjct: 345 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 404

Query: 181 TLDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLE 240
             D  +G    NC +  +P++   K+    +LGRPWR YS    IKSF+D+++ P GWLE
Sbjct: 405 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 464

Query: 241 W-DGVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPET 278
                    L+Y EY N G GA+ ++RV WPGF  + N  E   FTV  FI G+ WL  T
Sbjct: 465 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 524

BLAST of Lsi02G017320 vs. TAIR10
Match: AT4G33220.1 (AT4G33220.1 pectin methylesterase 44)

HSP 1 Score: 252.7 bits (644), Expect = 2.7e-67
Identity = 119/304 (39.14%), Postives = 181/304 (59.54%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           +LDGTGNF K+ DAI  AP++S+TRF I++K G Y E +E+  +K  I ++GD    T+I
Sbjct: 216 ALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVI 275

Query: 61  S----------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVF 120
           S                            +TF+N+AGP   QA+A+   +  + +++C  
Sbjct: 276 SGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSVFFRCAM 335

Query: 121 LGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTR--LPGVESTVTAQSKE 180
            GYQDTLY   + QF++EC I G++DFIFG+G V+FQ+C I  +  LP  ++T+TAQ ++
Sbjct: 336 RGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTITAQGRK 395

Query: 181 TLDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLE 240
            ++  SGF  Q   ++   ++    +    +LGRPW++YS   FI++ + +VV+P+GWLE
Sbjct: 396 DVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLE 455

Query: 241 WDG-VPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPET 274
           W+     + LFY E+ N G G+  S RV WPG+HV +N+ +   FTV  FI G  WLP T
Sbjct: 456 WNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGNLWLPST 515

BLAST of Lsi02G017320 vs. TAIR10
Match: AT2G26440.1 (AT2G26440.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 251.9 bits (642), Expect = 4.5e-67
Identity = 125/302 (41.39%), Postives = 177/302 (58.61%), Query Frame = 1

Query: 3   DGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTIIS- 62
           DGTGNF  +N+AI+ APN S  R  I+VK G Y+E I++P  KT I LIGD +  T I+ 
Sbjct: 243 DGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTFITG 302

Query: 63  ---------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVFLG 122
                                      +   N+AGP   QA+A+   A   A Y+CV  G
Sbjct: 303 NRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCVIDG 362

Query: 123 YQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRL--PGVESTVTAQSKETL 182
           YQDTLY  +  QF++EC+IYG+ID+IFGN  V+FQ CNI ++L  PG  + +TAQS++T 
Sbjct: 363 YQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQSRDTQ 422

Query: 183 DAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWD 242
           D  +G   QNC++  S ++ +S +KV  +LGRPWR +S    ++S++D  +   GW +W+
Sbjct: 423 DEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGWSKWN 482

Query: 243 GVPE-NLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 274
           G    + L+Y EYNN G G++T +RVNWPGFH++    +   FT   FI G  WL  T  
Sbjct: 483 GGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGDGWLGSTSF 542

BLAST of Lsi02G017320 vs. TAIR10
Match: AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 246.5 bits (628), Expect = 1.9e-65
Identity = 120/305 (39.34%), Postives = 177/305 (58.03%), Query Frame = 1

Query: 3   DGTGNFVKVNDAIAAAPNF---STTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTI 62
           +GTGNF  +N AIAAAPN    S   F I+V  G Y E +EVP  K ++ +IGD  + T+
Sbjct: 251 NGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTV 310

Query: 63  I----------------------------SLTFENSAGPNGGQAIAVLDKATHTAYYKCV 122
           I                            ++T  N+AGP  GQA+A+      + +Y C 
Sbjct: 311 ITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCS 370

Query: 123 FLGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLP--GVESTVTAQSK 182
           F  YQDTLY  ++ QF++EC++YG++DFIFGN  V+ Q+CN+Y R P  G  + VTAQ +
Sbjct: 371 FEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGR 430

Query: 183 ETLDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWL 242
              +  +G     CT+  + ++A+S   V  +LGRPW+ YS    +++++D  ++P GW 
Sbjct: 431 TDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWN 490

Query: 243 EWDG-VPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPE 274
            W G    + L+YAEYNN G G+DT+ RV WPG+HV+ NA + + FTV  F+ G  W+ +
Sbjct: 491 AWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQ 550

BLAST of Lsi02G017320 vs. TAIR10
Match: AT3G05620.1 (AT3G05620.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 241.5 bits (615), Expect = 6.1e-64
Identity = 123/304 (40.46%), Postives = 178/304 (58.55%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           ++DG G +  +N+AI  APN ST R+ I+VK G Y E I++  +KT I L+GD    TII
Sbjct: 244 AIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIGQTII 303

Query: 61  S----------------------------LTFENSAGPNGGQAIAVLDKATHTAYYKCVF 120
           +                            +TF N+AGP   QA+A+   +  +A+Y+C  
Sbjct: 304 TGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAFYRCSV 363

Query: 121 LGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTR--LPGVESTVTAQSKE 180
            GYQDTLYA ++ QF+++C IYG+IDFIFGNG  + Q+C IYTR  LP  + T+TAQ ++
Sbjct: 364 EGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQGRK 423

Query: 181 TLDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLE 240
           + +  +GFV QN  V  +            +LGRPW++YS   ++ +++  +VQP+GWLE
Sbjct: 424 SPNQNTGFVIQNSYVLATQ---------PTYLGRPWKLYSRTVYMNTYMSQLVQPRGWLE 483

Query: 241 WDG-VPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPET 274
           W G    + L+Y EYNN G G  +S RV WPG+H++ +     +FTV  FI G  WLP T
Sbjct: 484 WFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIM-DKRTALSFTVGSFIDGRRWLPAT 537

BLAST of Lsi02G017320 vs. NCBI nr
Match: gi|778697621|ref|XP_011654361.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 463.4 bits (1191), Expect = 2.8e-127
Identity = 228/306 (74.51%), Postives = 253/306 (82.68%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           SLDGT NF+K+NDAIAAAPNFSTTRFYIHVKPGTY EIIEVP +KT IALIGDD+STTII
Sbjct: 33  SLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTII 92

Query: 61  ----------------------------SLTFENSAGPNG-GQAIAVLDKATHTAYYKCV 120
                                        LTF+N+AG    GQAIA+LD+ATHTAYYKCV
Sbjct: 93  VNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAG-PNEGQAIALLDQATHTAYYKCV 152

Query: 121 FLGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPGVESTVTAQSKET 180
           FLGYQDTLYAGA+PQFFKEC+IYGS+DFIFGNGLV+FQDCNIY RL  V+ TVTAQSK +
Sbjct: 153 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 212

Query: 181 LDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEW 240
           L+++SGF+FQNC VTVSPEIASSKDKVTVFLGRPWR+YSMV FI SFLDNVVQPKGWLEW
Sbjct: 213 LNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 272

Query: 241 DGVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 278
            GVPENLL+YAEYNN G GA+TSQRVNWPG+H L+NA EVATFTVEIFI+GT WLP+TGI
Sbjct: 273 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVEIFINGTQWLPQTGI 332

BLAST of Lsi02G017320 vs. NCBI nr
Match: gi|700198848|gb|KGN54006.1| (hypothetical protein Csa_4G260430 [Cucumis sativus])

HSP 1 Score: 463.4 bits (1191), Expect = 2.8e-127
Identity = 228/306 (74.51%), Postives = 253/306 (82.68%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           SLDGT NF+K+NDAIAAAPNFSTTRFYIHVKPGTY EIIEVP +KT IALIGDD+STTII
Sbjct: 26  SLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTII 85

Query: 61  ----------------------------SLTFENSAGPNG-GQAIAVLDKATHTAYYKCV 120
                                        LTF+N+AG    GQAIA+LD+ATHTAYYKCV
Sbjct: 86  VNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAG-PNEGQAIALLDQATHTAYYKCV 145

Query: 121 FLGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPGVESTVTAQSKET 180
           FLGYQDTLYAGA+PQFFKEC+IYGS+DFIFGNGLV+FQDCNIY RL  V+ TVTAQSK +
Sbjct: 146 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 205

Query: 181 LDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEW 240
           L+++SGF+FQNC VTVSPEIASSKDKVTVFLGRPWR+YSMV FI SFLDNVVQPKGWLEW
Sbjct: 206 LNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 265

Query: 241 DGVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 278
            GVPENLL+YAEYNN G GA+TSQRVNWPG+H L+NA EVATFTVEIFI+GT WLP+TGI
Sbjct: 266 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVEIFINGTQWLPQTGI 325

BLAST of Lsi02G017320 vs. NCBI nr
Match: gi|659131731|ref|XP_008465826.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 463.0 bits (1190), Expect = 3.7e-127
Identity = 225/305 (73.77%), Postives = 248/305 (81.31%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           SLDGTGNFVKVNDAIAAAPNFS TRFYIHVKPGTY EIIEVPS+KTFIALIGDD+STTII
Sbjct: 20  SLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSSTTII 79

Query: 61  SLTFENSAGPNG----------------------------GQAIAVLDKATHTAYYKCVF 120
                N  G +                             GQAIAVLD+A HTAYY+CVF
Sbjct: 80  VNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCVF 139

Query: 121 LGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPGVESTVTAQSKETL 180
           LGYQDTLYAG+VPQFFKEC+IYGS+DFIFGNGLV+FQDCNIY RL  V+ TVTAQSK++L
Sbjct: 140 LGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQSL 199

Query: 181 DAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWD 240
            A SGF+FQNC VTVSPEIASSKDKVTVFLGRPWR+YSMV FI SFLD+VVQPKGWLEW 
Sbjct: 200 SAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEWP 259

Query: 241 GVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGIP 278
           GVPEN+L+YAEYNN G GA+TSQRVNWPG+HVL NA EVATF+VE+FI+GT WLP+TGIP
Sbjct: 260 GVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGIP 319

BLAST of Lsi02G017320 vs. NCBI nr
Match: gi|778708551|ref|XP_011656233.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 462.6 bits (1189), Expect = 4.8e-127
Identity = 228/306 (74.51%), Postives = 251/306 (82.03%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           SLDGTGNFVK+NDAIAAAPNFSTTRFYIHVKPGTY EIIEVP +KT IALIGDDASTTII
Sbjct: 33  SLDGTGNFVKINDAIAAAPNFSTTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDASTTII 92

Query: 61  ----------------------------SLTFENSAGPNG-GQAIAVLDKATHTAYYKCV 120
                                        LTF+N+AG    GQAIA+LD+ATHTAYYKCV
Sbjct: 93  VNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAG-PNEGQAIALLDQATHTAYYKCV 152

Query: 121 FLGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPGVESTVTAQSKET 180
           FLGYQDTLYAGA+PQFFKEC+IYGS+DFIFGNGLV+FQDCNIY RL  V+ TVTAQSK +
Sbjct: 153 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 212

Query: 181 LDAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEW 240
           L+++SGF+FQNC VTVSPEIA SKD VTVFLGRPWR+YSMV FI SFLDNVVQPKGWLEW
Sbjct: 213 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 272

Query: 241 DGVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGI 278
            GVPENLL+YAEYNN G GA+TSQRVNWPG+H L+NA EVATFTVE FI+GT WLP+TGI
Sbjct: 273 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI 332

BLAST of Lsi02G017320 vs. NCBI nr
Match: gi|778657958|ref|XP_004152582.2| (PREDICTED: pectinesterase 2-like [Cucumis sativus])

HSP 1 Score: 362.8 bits (930), Expect = 5.2e-97
Identity = 179/302 (59.27%), Postives = 213/302 (70.53%), Query Frame = 1

Query: 1   SLDGTGNFVKVNDAIAAAPNFSTTRFYIHVKPGTYNEIIEVPSQKTFIALIGDDASTTII 60
           SLDG+GNF +++DAIAAAPN S TRFYIHV PGTY+E +++P  K FIAL+GDD  TTII
Sbjct: 31  SLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDDPFTTII 90

Query: 61  ----------------------------SLTFENSAGPNGGQAIAVLDKATHTAYYKCVF 120
                                       SLTFENSAGP   QA+AV DKA HTAYYKC F
Sbjct: 91  IDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKCRF 150

Query: 121 LGYQDTLYAGAVPQFFKECNIYGSIDFIFGNGLVIFQDCNIYTRLPGVESTVTAQSKETL 180
           L +QDTLY    PQFFKE +IYGS+DFI G G V+FQDCNIY R+P    TVTAQSK  L
Sbjct: 151 LSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTAQSKYIL 210

Query: 181 DAMSGFVFQNCTVTVSPEIASSKDKVTVFLGRPWRIYSMVTFIKSFLDNVVQPKGWLEWD 240
            ++SGF FQNCTVTVS EI+S+K  V VFLGRPW+ YS V F++SFLD+VV  +GW+EW 
Sbjct: 211 RSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEWI 270

Query: 241 GVPENLLFYAEYNNRGAGADTSQRVNWPGFHVLHNAHEVATFTVEIFIHGTHWLPETGIP 275
           GVP N LFY E+NN G GAD S+RVNW  +H+L +      FTV+ F++G+ WLPETGIP
Sbjct: 271 GVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLL-DKESALRFTVDNFVNGSEWLPETGIP 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME39_ARATH9.2e-7042.86Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=P... [more]
PME44_ARATH4.7e-6639.14Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=P... [more]
PME12_ARATH8.1e-6641.39Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana GN=P... [more]
PME2_CITSI2.3e-6540.86Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME_PRUPE5.2e-6539.09Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1M3_CUCSA2.0e-12774.51Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1[more]
A0A0A0LR14_CUCSA3.6e-9759.27Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1[more]
A0A072U3V6_MEDTR2.6e-7143.38Pectinesterase OS=Medicago truncatula GN=MTR_7g108905 PE=4 SV=1[more]
A0A151U4L2_CAJCA6.4e-7042.72Pectinesterase OS=Cajanus cajan GN=KK1_006907 PE=4 SV=1[more]
A0A0D2SCA1_GOSRA7.1e-6941.72Pectinesterase OS=Gossypium raimondii GN=B456_007G127000 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02300.15.2e-7142.86 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G33220.12.7e-6739.14 pectin methylesterase 44[more]
AT2G26440.14.5e-6741.39 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G47550.11.9e-6539.34 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G05620.16.1e-6440.46 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|778697621|ref|XP_011654361.1|2.8e-12774.51PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|700198848|gb|KGN54006.1|2.8e-12774.51hypothetical protein Csa_4G260430 [Cucumis sativus][more]
gi|659131731|ref|XP_008465826.1|3.7e-12773.77PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
gi|778708551|ref|XP_011656233.1|4.8e-12774.51PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|778657958|ref|XP_004152582.2|5.2e-9759.27PREDICTED: pectinesterase 2-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR018040Pectinesterase_Tyr_AS
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0044765 single-organism transport
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G017320.1Lsi02G017320.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 62..262
score: 1.2E-73coord: 3..61
score: 4.7
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 3..273
score: 8.97
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 62..274
score: 1.7E-73coord: 3..61
score: 2.6
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 22..41
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 1..277
score: 1.4E