CmaCh05G009180 (gene) Cucurbita maxima (Rimu)

NameCmaCh05G009180
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPectinesterase
LocationCma_Chr05 : 7332386 .. 7333457 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTAGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACAGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCTATTCAGGTGAAACCTGGAACATACAACGAGGTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGAGGATGCTTCTACCACCATCATCGTTGACGATCGAAGTAATGGCACTGGATCGACCACAGCAACTTCTGCAACTCTCAGTAAGCTTCAAATTCTCCGCTCACTGATTAAATTTGTGTTAATACTAAAACATATTTCATTGTATAGCTGTAAGAGGTGCAAATTTCATGGCTCAGTCGTTGACATTTCAAAACTCCGCTGGGCCGAAGGAGCACCAAGCCGTTGCTGTTCTTGATCAAGCCAGACACACATCATATTATAAATGTGTGTTTTTGGGTTATCAAGATACGCTCTATGCTGGGGGACTACCCCAATTCTTCAAAGAGTGCGATATATATGGAAGCATTGACTTCATATTCGGAAACGGAGTCTCCGTATTCCAAGATTGTAATATATATGTTCGAGAATTTGGTATTAAGACTACGGTAACAGCACAATCGAAAGCAAATATAGATGATCAAAGTGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGGCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAGAGAATATTCAACGGTTGTGTTCATCGAGTCATTTCTTGACAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAAATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCGGTGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

mRNA sequence

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTAGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACAGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCTATTCAGGTGAAACCTGGAACATACAACGAGGTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGAGGATGCTTCTACCACCATCATCGTTGACGATCGAAGTAATGGCACTGGATCGACCACAGCAACTTCTGCAACTCTCACTGTAAGAGGTGCAAATTTCATGGCTCAGTCGTTGACATTTCAAAACTCCGCTGGGCCGAAGGAGCACCAAGCCGTTGCTGTTCTTGATCAAGCCAGACACACATCATATTATAAATGTGTGTTTTTGGGTTATCAAGATACGCTCTATGCTGGGGGACTACCCCAATTCTTCAAAGAGTGCGATATATATGGAAGCATTGACTTCATATTCGGAAACGGAGTCTCCGTATTCCAAGATTGTAATATATATGTTCGAGAATTTGGTATTAAGACTACGGTAACAGCACAATCGAAAGCAAATATAGATGATCAAAGTGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGGCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAGAGAATATTCAACGGTTGTGTTCATCGAGTCATTTCTTGACAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAAATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCGGTGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

Coding sequence (CDS)

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTAGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACAGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCTATTCAGGTGAAACCTGGAACATACAACGAGGTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGAGGATGCTTCTACCACCATCATCGTTGACGATCGAAGTAATGGCACTGGATCGACCACAGCAACTTCTGCAACTCTCACTGTAAGAGGTGCAAATTTCATGGCTCAGTCGTTGACATTTCAAAACTCCGCTGGGCCGAAGGAGCACCAAGCCGTTGCTGTTCTTGATCAAGCCAGACACACATCATATTATAAATGTGTGTTTTTGGGTTATCAAGATACGCTCTATGCTGGGGGACTACCCCAATTCTTCAAAGAGTGCGATATATATGGAAGCATTGACTTCATATTCGGAAACGGAGTCTCCGTATTCCAAGATTGTAATATATATGTTCGAGAATTTGGTATTAAGACTACGGTAACAGCACAATCGAAAGCAAATATAGATGATCAAAGTGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGGCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAGAGAATATTCAACGGTTGTGTTCATCGAGTCATTTCTTGACAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAAATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCGGTGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

Protein sequence

MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQPKGWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSRWLPETGIPFRTGF
BLAST of CmaCh05G009180 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.2e-79
Identity = 141/310 (45.48%), Postives = 206/310 (66.45%), Query Frame = 1

Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGEDAS 85
           ++ V+ DG+GN   I +A+AAA      R+ I +K GTYNE IEV      I  +G+   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 86  TTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTSYY 145
            TII   +S G G+TT  SAT+ V G NF+A+ +T +N+AGP  HQAVA+   +  + +Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 146 KCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGIKT-TVTAQ 205
           +C F GYQDTLY     QF++ECDIYG++DFIFGN   V Q+CNI+ R+   +T T+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 206 SKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQPKG 265
            + + +  +G +  NC+VT A ++   +  V  +LGRPW++YS  V+I++FLDS++ P G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 266 WLGWP-NVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSRWL 325
           W+ W  +  ++ L++AEY NTGPG+ T+ RV W GYH+L + +QV++FTV NFI G+ WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 326 PETGIPFRTG 334
           P T +PF +G
Sbjct: 502 PATNVPFTSG 509

BLAST of CmaCh05G009180 vs. Swiss-Prot
Match: PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=PME39 PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.0e-79
Identity = 142/314 (45.22%), Postives = 204/314 (64.97%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGED 83
           +++++V++DGTGN+  I+DA+ AAPN S  RF I +K G Y E +E+P  KT I  IG+ 
Sbjct: 219 NYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDG 278

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTS 143
              T+I  +RS   G +T  + T+ V+G  ++A+ ++F NSAGP + QAVA    + H++
Sbjct: 279 IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSA 338

Query: 144 YYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVRE--FGIKTTV 203
           +Y+C F GYQDTLY     QF++ECDIYG+IDFIFGN   VFQ+ ++Y R+   G K   
Sbjct: 339 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 398

Query: 204 TAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQ 263
           TAQS+   D  +G    NC++  AP++   K+    YLGRPWR+YS  V I+SF+D ++ 
Sbjct: 399 TAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIH 458

Query: 264 PKGWL-GWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGS 323
           P GWL G  +  ++ L++ EY N GPGA+ ++RV WPG+  +   T+ T+FTV  FI GS
Sbjct: 459 PAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGS 518

Query: 324 RWLPETGIPFRTGF 335
            WL  TGIPF  GF
Sbjct: 519 TWLNSTGIPFSLGF 532

BLAST of CmaCh05G009180 vs. Swiss-Prot
Match: PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 5.5e-77
Identity = 137/312 (43.91%), Postives = 196/312 (62.82%), Query Frame = 1

Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGEDAS 85
           D  V+ DGTGN+  ISDA+ AAP++STKR+ I VK G Y E +E+   K  I ++G+   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 86  TTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTSYY 145
            T+I  +RS   G TT  SAT  V G  F+A+ +TFQN+AGP++HQAVA+        +Y
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFY 333

Query: 146 KCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGI---KTTVT 205
           +C   GYQDTLYA  + QFF+EC I G++DFIFG+  +VFQ C I  ++ G+   K ++T
Sbjct: 334 RCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQ-GLPNQKNSIT 393

Query: 206 AQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQP 265
           AQ + + ++ +GF  Q   +    ++  + +    YLGRPW+ YS  VF+++++   + P
Sbjct: 394 AQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINP 453

Query: 266 KGWLGW-PNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSR 325
            GWL W  N  +D L++ EY N+GPGA   RRV WPGYH+L+T+ +   FTV   I G+ 
Sbjct: 454 VGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNL 513

Query: 326 WLPETGIPFRTG 334
           WLP TGI F  G
Sbjct: 514 WLPSTGITFIAG 524

BLAST of CmaCh05G009180 vs. Swiss-Prot
Match: PME44_ARATH (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=PME44 PE=2 SV=2)

HSP 1 Score: 285.8 bits (730), Expect = 6.1e-76
Identity = 137/314 (43.63%), Postives = 199/314 (63.38%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGED 83
           ++DV+V+LDGTGN+ KI DAI  AP++S+ RF I +K G Y E +E+   K  I ++G+ 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTS 143
              T+I  +RS   G TT  SAT  V G  F+A+ +TFQN+AGP++HQAVA+   +  + 
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 329

Query: 144 YYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGI---KTT 203
           +++C   GYQDTLY   + QF++EC I G++DFIFG+G  VFQ+C I  +  G+   K T
Sbjct: 330 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKR-GLPNQKNT 389

Query: 204 VTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVV 263
           +TAQ + +++  SGF  Q   ++   ++    +    YLGRPW+ YS  VFI + +  VV
Sbjct: 390 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVV 449

Query: 264 QPKGWLGW-PNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGG 323
           +P+GWL W  +  +D LF+ E+ N GPG+  S RV WPGYH+ + + Q   FTV  FI G
Sbjct: 450 RPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKG 509

Query: 324 SRWLPETGIPFRTG 334
           + WLP TG+ F  G
Sbjct: 510 NLWLPSTGVTFSDG 522

BLAST of CmaCh05G009180 vs. Swiss-Prot
Match: PME12_ARATH (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana GN=PME12 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.0e-75
Identity = 143/316 (45.25%), Postives = 194/316 (61.39%), Query Frame = 1

Query: 21  YCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALI 80
           Y  S  + V+ DGTGN+  I++AI+ APN S  R  I VK G Y+E I++P  KT I LI
Sbjct: 232 YDPSESLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLI 291

Query: 81  GEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAR 140
           G+ +  T I  +RS G G TT  SATL V G  F+A+ +   N+AGP++HQAVA+   A 
Sbjct: 292 GDGSDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNAD 351

Query: 141 HTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVR--EFGIK 200
             + Y+CV  GYQDTLY     QF++ECDIYG+ID+IFGN   VFQ CNI  +    G  
Sbjct: 352 FVALYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQF 411

Query: 201 TTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDS 260
           T +TAQS+   D+ +G   QNC +  + ++ +S +KV  YLGRPWRE+S  V +ES++D 
Sbjct: 412 TVITAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDE 471

Query: 261 VVQPKGWLGW-PNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFI 320
            +   GW  W     +D L++ EYNN GPG++T +RV+WPG+HI+        FT   FI
Sbjct: 472 FIDGSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFI 531

Query: 321 GGSRWLPETGIPFRTG 334
            G  WL  T  P+  G
Sbjct: 532 TGDGWLGSTSFPYDNG 546

BLAST of CmaCh05G009180 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 2.1e-139
Identity = 240/330 (72.73%), Postives = 285/330 (86.36%), Query Frame = 1

Query: 8   MLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTY 67
           M+F++YLLV     FS C+SF+ TVSLDGT N++KI+DAIAAAPNFST RF I VKPGTY
Sbjct: 1   MIFVLYLLVVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTY 60

Query: 68  NEVIEVPPSKTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNS 127
            E+IEVP  KT IALIG+D+STTIIV++RSNGTGS+TA+SATLTV G+NFMAQ LTFQN+
Sbjct: 61  QEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNT 120

Query: 128 AGPKEHQAVAVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSV 187
           AGP E QA+A+LDQA HT+YYKCVFLGYQDTLYAG LPQFFKECDIYGS+DFIFGNG+ V
Sbjct: 121 AGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVV 180

Query: 188 FQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWR 247
           FQDCNIY R F ++ TVTAQSK +++  SGF+FQNCKVTV+PEIASSKDKV ++LGRPWR
Sbjct: 181 FQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWR 240

Query: 248 EYSTVVFIESFLDSVVQPKGWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHT 307
            YS VVFI+SFLD+VVQPKGWL WP VP + L++AEYNN+G GA+TS+RV+WPGYH L+ 
Sbjct: 241 MYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNN 300

Query: 308 ATQVTRFTVENFIGGSRWLPETGIPFRTGF 335
           A +V  FTVE FI G++WLP+TGIPFR GF
Sbjct: 301 AKEVATFTVEIFINGTQWLPQTGIPFRAGF 330

BLAST of CmaCh05G009180 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.6e-110
Identity = 196/310 (63.23%), Postives = 240/310 (77.42%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGED 83
           SF++TVSLDG+GN+ +ISDAIAAAPN S  RF I V PGTY+E +++P  K FIAL+G+D
Sbjct: 25  SFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDD 84

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTS 143
             TTII+D+RSNGTG  T  SATLTV+G NFMA+SLTF+NSAGP+ +QAVAV D+A HT+
Sbjct: 85  PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTA 144

Query: 144 YYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGIKTTVTA 203
           YYKC FL +QDTLY  G PQFFKE DIYGS+DFI G G  +FQDCNIY R      TVTA
Sbjct: 145 YYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTA 204

Query: 204 QSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQPK 263
           QSK  +   SGF FQNC VTV+ EI+S+K  V ++LGRPW++YS VVF+ESFLD VV  +
Sbjct: 205 QSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASE 264

Query: 264 GWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSRWL 323
           GW+ W  VPV+ LF+ E+NN GPGAD S+RV+W  YH+L   + + RFTV+NF+ GS WL
Sbjct: 265 GWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESAL-RFTVDNFVNGSEWL 324

Query: 324 PETGIPFRTG 334
           PETGIPFR G
Sbjct: 325 PETGIPFRRG 333

BLAST of CmaCh05G009180 vs. TrEMBL
Match: A0A151U4L2_CAJCA (Pectinesterase OS=Cajanus cajan GN=KK1_006907 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.2e-80
Identity = 144/312 (46.15%), Postives = 208/312 (66.67%), Query Frame = 1

Query: 25  FDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGEDA 84
           FD+ V+ DGTGN+  I +A+A APN ST RF I +K G Y E +EV   KT +  +G+  
Sbjct: 157 FDLVVAKDGTGNFTTIGEALAVAPNSSTTRFVIHIKAGAYLENVEVIRKKTNLMFVGDGI 216

Query: 85  STTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTSY 144
             T++   R+   G TT  SAT+ V GA F+A+ +TF+NSAGP++HQAVA+   A  +++
Sbjct: 217 GKTVVKGSRNVVDGWTTFQSATVAVVGAGFIAKGITFENSAGPEKHQAVALRSGADFSAF 276

Query: 145 YKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVRE--FGIKTTVT 204
           Y+C F+GYQDTLY   L QF++ECDIYG++DFIFGN   VFQ+CN+Y R+     K   T
Sbjct: 277 YQCSFVGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYARKPNQNQKNLFT 336

Query: 205 AQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQP 264
           AQ + + +  +G    NCKV  A ++   K     YLGRPW+ YS  VF++S+++ +++P
Sbjct: 337 AQGREDPNQNTGISILNCKVAAAADLIPVKTSFKSYLGRPWKMYSRTVFLKSYMEDLIEP 396

Query: 265 KGWLGWPNV-PVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSR 324
            GWL W +   +D L++ EY N GPG++TS RV WPGY +++++T+ ++FTV  FI G+ 
Sbjct: 397 AGWLEWNDTFALDTLYYGEYMNRGPGSNTSGRVTWPGYRVINSSTEASQFTVGEFIQGND 456

Query: 325 WLPETGIPFRTG 334
           WL  TGIPF +G
Sbjct: 457 WLNSTGIPFFSG 468

BLAST of CmaCh05G009180 vs. TrEMBL
Match: A0A0B0P3D3_GOSAR (Pectinesterase OS=Gossypium arboreum GN=F383_07269 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.2e-80
Identity = 150/322 (46.58%), Postives = 214/322 (66.46%), Query Frame = 1

Query: 15  LVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFS-TKRFSIQVKPGTYNEVIEVPPS 74
           L+ + S   + ++ V+ DG+GNY  I DAI+AA   S + R+ I VK GTY E +E+   
Sbjct: 197 LLQSSSPASTANIVVAQDGSGNYKTIKDAISAASKRSGSGRYVIYVKAGTYKENVEIGSK 256

Query: 75  KTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAV 134
              I ++G+    TII   +S G GSTT  SAT+ V G  F+A+ +TF+N+AGP  HQAV
Sbjct: 257 LKNIMMVGDGIGKTIITGSKSVGGGSTTFNSATVAVVGDGFIARGITFRNTAGPTNHQAV 316

Query: 135 AVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVR 194
           A+   +  + +YKC F GYQDTLY     QF++ECDIYG++D+IFGN   VFQDCNIY R
Sbjct: 317 ALRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRECDIYGTVDWIFGNAAVVFQDCNIYAR 376

Query: 195 EFGIKT-TVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFI 254
               KT TVTAQ + + +  +G +  N +VT A ++   +  V  YLGRPW++YS  VF+
Sbjct: 377 NPPNKTNTVTAQGRTDPNQNTGIIIHNSRVTAASDLKPVQSSVKTYLGRPWKQYSRTVFM 436

Query: 255 ESFLDSVVQPKGWLGWP-NVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRF 314
           +++LDS++ P GW+ W  +  +  L++AEY NTGPG+ TS RV W GYH+L +A++V++F
Sbjct: 437 KTYLDSLINPAGWMEWDGDFALKTLYYAEYMNTGPGSSTSNRVKWGGYHVLKSASEVSKF 496

Query: 315 TVENFIGGSRWLPETGIPFRTG 334
           TV NF+ G+ WLP TG+PF +G
Sbjct: 497 TVGNFLAGNSWLPSTGVPFTSG 518

BLAST of CmaCh05G009180 vs. TrEMBL
Match: V4T5L9_9ROSI (Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10003299mg PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.2e-80
Identity = 145/312 (46.47%), Postives = 208/312 (66.67%), Query Frame = 1

Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGEDAS 85
           DV V+ D +GNY KISDAI AAP+FS K + I++K G Y + + V   KT + LIG   +
Sbjct: 7   DVIVAQDSSGNYTKISDAILAAPSFSRKPYYIKIKEGNYTKYVVVGREKTNLVLIGNGIN 66

Query: 86  TTIIVDDRSNGTGS-TTATSATLTVRGANFMAQSLTFQNSAGPKEH--QAVAVLDQARHT 145
            TII  ++S+G G  +T  +AT+ ++G  F+A ++TF+N+AGP +   QAVA+L +   +
Sbjct: 67  NTIISGNKSHGNGHLSTYQTATVAIKGPGFVAMNITFENTAGPDQETGQAVALLSEV-DS 126

Query: 146 SYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGIKT-TV 205
           ++Y+C F+GYQDTLYAG   QF++ECDIYG+IDFIFG+  +VFQ+CNIYVR  G    T+
Sbjct: 127 AFYRCKFVGYQDTLYAGRNKQFYRECDIYGTIDFIFGDASAVFQNCNIYVRFLGGNVKTI 186

Query: 206 TAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQ 265
           TA+ +   +   G +  NC +T A +    +  +  YLGRPWR YS  V +++FLD ++ 
Sbjct: 187 TAEGREEPEGNGGIIIHNCTITAAEDFPKDRTSIKTYLGRPWRNYSRTVILQTFLDEIID 246

Query: 266 PKGWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSR 325
           P+GWL W    VD +++AEYNN GPG++T+ R+ W GY IL    +   FTV+ FI G +
Sbjct: 247 PEGWLEWQGQSVDTIYYAEYNNRGPGSNTTGRIRWKGYRIL-KRLEAENFTVKKFISGDK 306

Query: 326 WLPETGIPFRTG 334
           W+P TGIPF  G
Sbjct: 307 WIPSTGIPFFPG 316

BLAST of CmaCh05G009180 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 297.4 bits (760), Expect = 1.1e-80
Identity = 142/314 (45.22%), Postives = 204/314 (64.97%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGED 83
           +++++V++DGTGN+  I+DA+ AAPN S  RF I +K G Y E +E+P  KT I  IG+ 
Sbjct: 219 NYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDG 278

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTS 143
              T+I  +RS   G +T  + T+ V+G  ++A+ ++F NSAGP + QAVA    + H++
Sbjct: 279 IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSA 338

Query: 144 YYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVRE--FGIKTTV 203
           +Y+C F GYQDTLY     QF++ECDIYG+IDFIFGN   VFQ+ ++Y R+   G K   
Sbjct: 339 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 398

Query: 204 TAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQ 263
           TAQS+   D  +G    NC++  AP++   K+    YLGRPWR+YS  V I+SF+D ++ 
Sbjct: 399 TAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIH 458

Query: 264 PKGWL-GWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGS 323
           P GWL G  +  ++ L++ EY N GPGA+ ++RV WPG+  +   T+ T+FTV  FI GS
Sbjct: 459 PAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGS 518

Query: 324 RWLPETGIPFRTGF 335
            WL  TGIPF  GF
Sbjct: 519 TWLNSTGIPFSLGF 532

BLAST of CmaCh05G009180 vs. TAIR10
Match: AT3G43270.1 (AT3G43270.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 289.3 bits (739), Expect = 3.1e-78
Identity = 137/312 (43.91%), Postives = 196/312 (62.82%), Query Frame = 1

Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGEDAS 85
           D  V+ DGTGN+  ISDA+ AAP++STKR+ I VK G Y E +E+   K  I ++G+   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 86  TTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTSYY 145
            T+I  +RS   G TT  SAT  V G  F+A+ +TFQN+AGP++HQAVA+        +Y
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFY 333

Query: 146 KCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGI---KTTVT 205
           +C   GYQDTLYA  + QFF+EC I G++DFIFG+  +VFQ C I  ++ G+   K ++T
Sbjct: 334 RCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQ-GLPNQKNSIT 393

Query: 206 AQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQP 265
           AQ + + ++ +GF  Q   +    ++  + +    YLGRPW+ YS  VF+++++   + P
Sbjct: 394 AQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINP 453

Query: 266 KGWLGW-PNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSR 325
            GWL W  N  +D L++ EY N+GPGA   RRV WPGYH+L+T+ +   FTV   I G+ 
Sbjct: 454 VGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNL 513

Query: 326 WLPETGIPFRTG 334
           WLP TGI F  G
Sbjct: 514 WLPSTGITFIAG 524

BLAST of CmaCh05G009180 vs. TAIR10
Match: AT4G33220.1 (AT4G33220.1 pectin methylesterase 44)

HSP 1 Score: 285.8 bits (730), Expect = 3.4e-77
Identity = 137/314 (43.63%), Postives = 199/314 (63.38%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGED 83
           ++DV+V+LDGTGN+ KI DAI  AP++S+ RF I +K G Y E +E+   K  I ++G+ 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTS 143
              T+I  +RS   G TT  SAT  V G  F+A+ +TFQN+AGP++HQAVA+   +  + 
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 329

Query: 144 YYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGI---KTT 203
           +++C   GYQDTLY   + QF++EC I G++DFIFG+G  VFQ+C I  +  G+   K T
Sbjct: 330 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKR-GLPNQKNT 389

Query: 204 VTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVV 263
           +TAQ + +++  SGF  Q   ++   ++    +    YLGRPW+ YS  VFI + +  VV
Sbjct: 390 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVV 449

Query: 264 QPKGWLGW-PNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGG 323
           +P+GWL W  +  +D LF+ E+ N GPG+  S RV WPGYH+ + + Q   FTV  FI G
Sbjct: 450 RPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKG 509

Query: 324 SRWLPETGIPFRTG 334
           + WLP TG+ F  G
Sbjct: 510 NLWLPSTGVTFSDG 522

BLAST of CmaCh05G009180 vs. TAIR10
Match: AT2G26440.1 (AT2G26440.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 283.5 bits (724), Expect = 1.7e-76
Identity = 143/316 (45.25%), Postives = 194/316 (61.39%), Query Frame = 1

Query: 21  YCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALI 80
           Y  S  + V+ DGTGN+  I++AI+ APN S  R  I VK G Y+E I++P  KT I LI
Sbjct: 232 YDPSESLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLI 291

Query: 81  GEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQAR 140
           G+ +  T I  +RS G G TT  SATL V G  F+A+ +   N+AGP++HQAVA+   A 
Sbjct: 292 GDGSDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNAD 351

Query: 141 HTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVR--EFGIK 200
             + Y+CV  GYQDTLY     QF++ECDIYG+ID+IFGN   VFQ CNI  +    G  
Sbjct: 352 FVALYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQF 411

Query: 201 TTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDS 260
           T +TAQS+   D+ +G   QNC +  + ++ +S +KV  YLGRPWRE+S  V +ES++D 
Sbjct: 412 TVITAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDE 471

Query: 261 VVQPKGWLGW-PNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFI 320
            +   GW  W     +D L++ EYNN GPG++T +RV+WPG+HI+        FT   FI
Sbjct: 472 FIDGSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFI 531

Query: 321 GGSRWLPETGIPFRTG 334
            G  WL  T  P+  G
Sbjct: 532 TGDGWLGSTSFPYDNG 546

BLAST of CmaCh05G009180 vs. TAIR10
Match: AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 282.7 bits (722), Expect = 2.9e-76
Identity = 138/313 (44.09%), Postives = 198/313 (63.26%), Query Frame = 1

Query: 27  VTVSLDGTGNYVKISDAIAAAPNF---STKRFSIQVKPGTYNEVIEVPPSKTFIALIGED 86
           VTV  +GTGN+  I+ AIAAAPN    S   F I V  G Y E +EVP +K ++ +IG+ 
Sbjct: 246 VTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDG 305

Query: 87  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTS 146
            + T+I  +RS   G TT  SAT  + G NF+  ++T +N+AGP + QAVA+      + 
Sbjct: 306 INQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSV 365

Query: 147 YYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVRE--FGIKTTV 206
           +Y C F  YQDTLY   L QF++ECD+YG++DFIFGN   V Q+CN+Y R+   G    V
Sbjct: 366 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEV 425

Query: 207 TAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQ 266
           TAQ + + +  +G     C +  A ++A+S   V  YLGRPW+EYS  V +++++D  ++
Sbjct: 426 TAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLE 485

Query: 267 PKGWLGWP-NVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGS 326
           P GW  W  +  +  L++AEYNNTGPG+DT+ RV WPGYH+++ AT  + FTV NF+ G 
Sbjct: 486 PSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVIN-ATDASNFTVTNFLVGE 545

Query: 327 RWLPETGIPFRTG 334
            W+ +TG+PF  G
Sbjct: 546 GWIGQTGVPFVGG 557

BLAST of CmaCh05G009180 vs. NCBI nr
Match: gi|778697621|ref|XP_011654361.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 507.3 bits (1305), Expect = 2.0e-140
Identity = 243/337 (72.11%), Postives = 288/337 (85.46%), Query Frame = 1

Query: 1   MPSFKLAMLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSI 60
           M  F L M+F++YLLV     FS C+SF+ TVSLDGT N++KI+DAIAAAPNFST RF I
Sbjct: 1   MTIFTLEMIFVLYLLVVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYI 60

Query: 61  QVKPGTYNEVIEVPPSKTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQ 120
            VKPGTY E+IEVP  KT IALIG+D+STTIIV++RSNGTGS+TA+SATLTV G+NFMAQ
Sbjct: 61  HVKPGTYQEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQ 120

Query: 121 SLTFQNSAGPKEHQAVAVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFI 180
            LTFQN+AGP E QA+A+LDQA HT+YYKCVFLGYQDTLYAG LPQFFKECDIYGS+DFI
Sbjct: 121 FLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFI 180

Query: 181 FGNGVSVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLL 240
           FGNG+ VFQDCNIY R F ++ TVTAQSK +++  SGF+FQNCKVTV+PEIASSKDKV +
Sbjct: 181 FGNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTV 240

Query: 241 YLGRPWREYSTVVFIESFLDSVVQPKGWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWP 300
           +LGRPWR YS VVFI+SFLD+VVQPKGWL WP VP + L++AEYNN+G GA+TS+RV+WP
Sbjct: 241 FLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWP 300

Query: 301 GYHILHTATQVTRFTVENFIGGSRWLPETGIPFRTGF 335
           GYH L+ A +V  FTVE FI G++WLP+TGIPFR GF
Sbjct: 301 GYHALNNAKEVATFTVEIFINGTQWLPQTGIPFRAGF 337

BLAST of CmaCh05G009180 vs. NCBI nr
Match: gi|778708551|ref|XP_011656233.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 505.8 bits (1301), Expect = 6.0e-140
Identity = 244/332 (73.49%), Postives = 283/332 (85.24%), Query Frame = 1

Query: 6   LAMLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPG 65
           L MLFIVYLLV     FS C+ F+ TVSLDGTGN+VKI+DAIAAAPNFST RF I VKPG
Sbjct: 6   LKMLFIVYLLVVVIVEFSDCFIFNFTVSLDGTGNFVKINDAIAAAPNFSTTRFYIHVKPG 65

Query: 66  TYNEVIEVPPSKTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQ 125
           TY E+IEVP  KT IALIG+DASTTIIV++RSNGTGS+TA+SATLTV GANFMAQ LTFQ
Sbjct: 66  TYKEIIEVPYEKTCIALIGDDASTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQ 125

Query: 126 NSAGPKEHQAVAVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGV 185
           N+AGP E QA+A+LDQA HT+YYKCVFLGYQDTLYAG LPQFFKECDIYGS+DFIFGNG+
Sbjct: 126 NTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGL 185

Query: 186 SVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRP 245
            VFQDCNIY R F ++ TVTAQSK +++  SGF+FQNC VTV+PEIA SKD V ++LGRP
Sbjct: 186 VVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRP 245

Query: 246 WREYSTVVFIESFLDSVVQPKGWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHIL 305
           WR YS VVFI+SFLD+VVQPKGWL WP VP + L++AEYNN+G GA+TS+RV+WPGYH L
Sbjct: 246 WRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHAL 305

Query: 306 HTATQVTRFTVENFIGGSRWLPETGIPFRTGF 335
           + A +V  FTVE FI G++WLP+TGIPFR GF
Sbjct: 306 NNAKEVATFTVETFINGTQWLPQTGIPFRAGF 337

BLAST of CmaCh05G009180 vs. NCBI nr
Match: gi|700198848|gb|KGN54006.1| (hypothetical protein Csa_4G260430 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 3.0e-139
Identity = 240/330 (72.73%), Postives = 285/330 (86.36%), Query Frame = 1

Query: 8   MLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTY 67
           M+F++YLLV     FS C+SF+ TVSLDGT N++KI+DAIAAAPNFST RF I VKPGTY
Sbjct: 1   MIFVLYLLVVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTY 60

Query: 68  NEVIEVPPSKTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNS 127
            E+IEVP  KT IALIG+D+STTIIV++RSNGTGS+TA+SATLTV G+NFMAQ LTFQN+
Sbjct: 61  QEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNT 120

Query: 128 AGPKEHQAVAVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSV 187
           AGP E QA+A+LDQA HT+YYKCVFLGYQDTLYAG LPQFFKECDIYGS+DFIFGNG+ V
Sbjct: 121 AGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVV 180

Query: 188 FQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWR 247
           FQDCNIY R F ++ TVTAQSK +++  SGF+FQNCKVTV+PEIASSKDKV ++LGRPWR
Sbjct: 181 FQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWR 240

Query: 248 EYSTVVFIESFLDSVVQPKGWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHT 307
            YS VVFI+SFLD+VVQPKGWL WP VP + L++AEYNN+G GA+TS+RV+WPGYH L+ 
Sbjct: 241 MYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNN 300

Query: 308 ATQVTRFTVENFIGGSRWLPETGIPFRTGF 335
           A +V  FTVE FI G++WLP+TGIPFR GF
Sbjct: 301 AKEVATFTVEIFINGTQWLPQTGIPFRAGF 330

BLAST of CmaCh05G009180 vs. NCBI nr
Match: gi|659131731|ref|XP_008465826.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 486.5 bits (1251), Expect = 3.7e-134
Identity = 227/306 (74.18%), Postives = 267/306 (87.25%), Query Frame = 1

Query: 29  VSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGEDASTTI 88
           +SLDGTGN+VK++DAIAAAPNFS  RF I VKPGTY E+IEVP  KTFIALIG+D+STTI
Sbjct: 19  LSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSSTTI 78

Query: 89  IVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTSYYKCV 148
           IV++RSNGTGS+TA+SATLTV G NFMAQ LTFQNSAGP E QA+AVLDQA+HT+YY+CV
Sbjct: 79  IVNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCV 138

Query: 149 FLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGIKTTVTAQSKAN 208
           FLGYQDTLYAG +PQFFKECDIYGS+DFIFGNG+ VFQDCNIY R F ++ TVTAQSK +
Sbjct: 139 FLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQS 198

Query: 209 IDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQPKGWLGW 268
           +   SGF+FQNC VTV+PEIASSKDKV ++LGRPWR YS VVFI+SFLDSVVQPKGWL W
Sbjct: 199 LSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEW 258

Query: 269 PNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSRWLPETGI 328
           P VP + L++AEYNN+G GA+TS+RV+WPGYH+L  A +V  F+VE FI G++WLP+TGI
Sbjct: 259 PGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGI 318

Query: 329 PFRTGF 335
           PFR GF
Sbjct: 319 PFRAGF 324

BLAST of CmaCh05G009180 vs. NCBI nr
Match: gi|778657958|ref|XP_004152582.2| (PREDICTED: pectinesterase 2-like [Cucumis sativus])

HSP 1 Score: 406.8 bits (1044), Expect = 3.8e-110
Identity = 196/310 (63.23%), Postives = 240/310 (77.42%), Query Frame = 1

Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVKPGTYNEVIEVPPSKTFIALIGED 83
           SF++TVSLDG+GN+ +ISDAIAAAPN S  RF I V PGTY+E +++P  K FIAL+G+D
Sbjct: 25  SFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDD 84

Query: 84  ASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQNSAGPKEHQAVAVLDQARHTS 143
             TTII+D+RSNGTG  T  SATLTV+G NFMA+SLTF+NSAGP+ +QAVAV D+A HT+
Sbjct: 85  PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTA 144

Query: 144 YYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVSVFQDCNIYVREFGIKTTVTA 203
           YYKC FL +QDTLY  G PQFFKE DIYGS+DFI G G  +FQDCNIY R      TVTA
Sbjct: 145 YYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTA 204

Query: 204 QSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLGRPWREYSTVVFIESFLDSVVQPK 263
           QSK  +   SGF FQNC VTV+ EI+S+K  V ++LGRPW++YS VVF+ESFLD VV  +
Sbjct: 205 QSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASE 264

Query: 264 GWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSRWL 323
           GW+ W  VPV+ LF+ E+NN GPGAD S+RV+W  YH+L   + + RFTV+NF+ GS WL
Sbjct: 265 GWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESAL-RFTVDNFVNGSEWL 324

Query: 324 PETGIPFRTG 334
           PETGIPFR G
Sbjct: 325 PETGIPFRRG 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME2_CITSI1.2e-7945.48Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME39_ARATH2.0e-7945.22Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=P... [more]
PME32_ARATH5.5e-7743.91Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=P... [more]
PME44_ARATH6.1e-7643.63Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=P... [more]
PME12_ARATH3.0e-7545.25Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0L1M3_CUCSA2.1e-13972.73Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1[more]
A0A0A0LR14_CUCSA2.6e-11063.23Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1[more]
A0A151U4L2_CAJCA2.2e-8046.15Pectinesterase OS=Cajanus cajan GN=KK1_006907 PE=4 SV=1[more]
A0A0B0P3D3_GOSAR2.2e-8046.58Pectinesterase OS=Gossypium arboreum GN=F383_07269 PE=4 SV=1[more]
V4T5L9_9ROSI2.2e-8046.47Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10003299mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02300.11.1e-8045.22 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G43270.13.1e-7843.91 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G33220.13.4e-7743.63 pectin methylesterase 44[more]
AT2G26440.11.7e-7645.25 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G47550.12.9e-7644.09 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|778697621|ref|XP_011654361.1|2.0e-14072.11PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|778708551|ref|XP_011656233.1|6.0e-14073.49PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|700198848|gb|KGN54006.1|3.0e-13972.73hypothetical protein Csa_4G260430 [Cucumis sativus][more]
gi|659131731|ref|XP_008465826.1|3.7e-13474.18PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
gi|778657958|ref|XP_004152582.2|3.8e-11063.23PREDICTED: pectinesterase 2-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G009180.1CmaCh05G009180.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 26..319
score: 1.0E
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 21..332
score: 1.01
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 22..331
score: 4.6E
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 26..334
score: 4.0E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh05G009180CmaCh12G007210Cucurbita maxima (Rimu)cmacmaB201
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh05G009180Silver-seed gourdcarcmaB0174
CmaCh05G009180Silver-seed gourdcarcmaB1468
CmaCh05G009180Cucumber (Gy14) v1cgycmaB0771
CmaCh05G009180Cucurbita moschata (Rifu)cmacmoB788
CmaCh05G009180Cucurbita pepo (Zucchini)cmacpeB767
CmaCh05G009180Cucurbita pepo (Zucchini)cmacpeB800