Cp4.1LG11g07420 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG11g07420
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Pectinesterase
Location	Cp4.1LG11 : 5790846 .. 5791917 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTTGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACGGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCCATTCATGTGAAACTTGGAACATACAAGGAGTTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATTATCGTTGACGATCGAAGTAATGGCACTGGATCGACCACAGCAACTTCTGCAACTCTTAGTAAGCTTCAAATTCTCCGCTAACCGATTAAATTTGTGTTAATACTAAAACATATTTCATTTTATAGCTGTAAGAGGTGCAAATTTCATGGCTCAGTCGTTGACATTTCAAAACTCCGCTGGGCCGAAGGAGCACCAAGCTGTTGCAGTTCTTGACCAAGCCAAACACACAGCATACTATAAGTGTGTGTTTTTGGGTTATCAAGATACGCTCTATGCTGGGGGACTACCCCAATTCTTCAGAGAGTGCGATATATACGGAAGCATTGACTTCATATTCGGAAACGGAGTCACCGTATTCCAAGATTGTAATATATATGTTCGAGAATTTGGTAGTAAGACTACGGTAACAGCACAATCGAAAGCAAATATAGATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTTATCGAGTCATTTCTTGATAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAATGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

mRNA sequence

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTTGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACGGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCCATTCATGTGAAACTTGGAACATACAAGGAGTTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATTATCGTTGACGATCGAAATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTTATCGAGTCATTTCTTGATAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAATGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

Coding sequence (CDS)

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTTGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACGGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCCATTCATGTGAAACTTGGAACATACAAGGAGTTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATTATCGTTGACGATCGAAATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTTATCGAGTCATTTCTTGATAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAATGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

Protein sequence

MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDDASTTIIVDDRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIPFRTGF

Homology

[Close]

BLAST of Cp4.1LG11g07420 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.2e-35
Identity = 60/127 (47.24%), Postives = 89/127 (70.08%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G +  NC+VT + ++   +  V  +LGRPWK+YS  V+I++FLDS++ P GW+ 
Sbjct: 383 DPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWME 442

Query: 152 WP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  ++ L++AEY NTGPG+ T+ RV W GYH+L + +QV++FTV NFI G+ WLP T
Sbjct: 443 WSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPAT 502

Query: 212 GIPFRTG 218
            +PF +G
Sbjct: 503 NVPFTSG 509

[Close]

BLAST of Cp4.1LG11g07420 vs. Swiss-Prot
Match: PME20_ARATH (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=PME20 PE=2 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 6.0e-32
Identity = 57/127 (44.88%), Postives = 83/127 (65.35%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G     C +  + ++A+S   V  YLGRPWKEYS  V +++++D  ++P GW  
Sbjct: 432 DPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNA 491

Query: 152 WP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  +  L++AEYNNTGPG+DT+ RV WPGYH+++ AT  + FTV NF+ G  W+ +T
Sbjct: 492 WSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVIN-ATDASNFTVTNFLVGEGWIGQT 551

Query: 212 GIPFRTG 218
           G+PF  G
Sbjct: 552 GVPFVGG 557

[Close]

BLAST of Cp4.1LG11g07420 vs. Swiss-Prot
Match: PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=PME41 PE=2 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 1.0e-31
Identity = 58/127 (45.67%), Postives = 84/127 (66.14%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G    NC +  + ++ SS   V  YLGRPWKEYS  VF++S++D VV+P GW  
Sbjct: 445 DPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWRE 504

Query: 152 W-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  +  L++AEYNNTG G+ T+ RV WPGYH+++ +T    FTVENF+ G  W+ ++
Sbjct: 505 WNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVIN-STDANNFTVENFLLGDGWMVQS 564

Query: 212 GIPFRTG 218
           G+P+ +G
Sbjct: 565 GVPYISG 570

[Close]

BLAST of Cp4.1LG11g07420 vs. Swiss-Prot
Match: PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=PME39 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.9e-30
Identity = 57/129 (44.19%), Postives = 84/129 (65.12%), Query Frame = 1


Query: 92  DRNDQ-SGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWL 151
           +++DQ +G    NC++  +P++   K+    YLGRPW++YS  V I+SF+D ++ P GWL
Sbjct: 404 NQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWL 463

Query: 152 -GWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPE 211
            G  D  ++ L++ EY N GPGA+ ++RV WPG+  +   T+ T+FTV  FI GS WL  
Sbjct: 464 EGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNS 523

Query: 212 TGIPFRTGF 219
           TGIPF  GF
Sbjct: 524 TGIPFSLGF 532

[Close]

BLAST of Cp4.1LG11g07420 vs. Swiss-Prot
Match: PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PME7 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 4.3e-30
Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G   QNC +  + ++ SS   V  YLGRPWKEYS  V+++S++D  V+P GW  
Sbjct: 451 DPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWRE 510

Query: 152 W-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  +  L++AEYNNTGPG++T+ RV WPGYH+++ +T    FTV      + W+ +T
Sbjct: 511 WNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVIN-STDAANFTVTGLFIEADWIWKT 570

Query: 212 GIPFRTG 218
           G+P+ +G
Sbjct: 571 GVPYTSG 576

[Close]

BLAST of Cp4.1LG11g07420 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.6e-50
Identity = 90/125 (72.00%), Postives = 107/125 (85.60%), Query Frame = 1


Query: 94  NDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWP 153
           N  SGF+FQNCKVTVSPEIASSKDKV ++LGRPW+ YS VVFI+SFLD+VVQPKGWL WP
Sbjct: 206 NSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWP 265

Query: 154 DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIP 213
            VP + L++AEYNN+G GA+TS+RV+WPGYH L+ A +V  FTVE FI G+QWLP+TGIP
Sbjct: 266 GVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVEIFINGTQWLPQTGIP 325

Query: 214 FRTGF 219
           FR GF
Sbjct: 326 FRAGF 330

[Close]

BLAST of Cp4.1LG11g07420 vs. TrEMBL
Match: A0A0A0KQN0_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G488750 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 6.4e-49
Identity = 87/125 (69.60%), Postives = 104/125 (83.20%), Query Frame = 1


Query: 94  NDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWP 153
           N  SGF+FQNC VTVSPEIA SKD V ++LGRPW+ YS VVFI+SFLD+VVQPKGWL WP
Sbjct: 44  NSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWP 103

Query: 154 DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIP 213
            VP + L++AEYNN+G GA+TS+RV+WPGYH L+ A +V  FTVE FI G+QWLP+TGIP
Sbjct: 104 GVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGIP 163

Query: 214 FRTGF 219
           FR GF
Sbjct: 164 FRAGF 168

[Close]

BLAST of Cp4.1LG11g07420 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.4e-40
Identity = 76/121 (62.81%), Postives = 95/121 (78.51%), Query Frame = 1


Query: 97  SGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWPDVP 156
           SGF FQNC VTVS EI+S+K  V ++LGRPWK+YS VVF+ESFLD VV  +GW+ W  VP
Sbjct: 214 SGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEWIGVP 273

Query: 157 VDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIPFRT 216
           V+ LF+ E+NN GPGAD S+RV+W  YH+L   + + RFTV+NF+ GS+WLPETGIPFR 
Sbjct: 274 VNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESAL-RFTVDNFVNGSEWLPETGIPFRR 333

Query: 217 G 218
           G
Sbjct: 334 G 333

[Close]

BLAST of Cp4.1LG11g07420 vs. TrEMBL
Match: A0A067H5N1_CITSI (Pectinesterase OS=Citrus sinensis GN=CISIN_1g043374mg PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 5.3e-35
Identity = 64/128 (50.00%), Postives = 86/128 (67.19%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G    NC +  S ++AS    V  YLGRPWKEYS  V++++F+DS++ P GW  
Sbjct: 439 DPNQNTGTSIHNCTIRASNDLASGSQTVQTYLGRPWKEYSRTVYMQTFMDSLINPAGWHD 498

Query: 152 WP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  +  L++AEYNNTGPG+DT+ RV WPGYHI+  AT+   FTV NF+ G  WLP+T
Sbjct: 499 WSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHII-DATEAANFTVSNFLSGDNWLPQT 558

Query: 212 GIPFRTGF 219
           G+P+  GF
Sbjct: 559 GVPYNGGF 565

[Close]

BLAST of Cp4.1LG11g07420 vs. TrEMBL
Match: V4TBH6_9ROSI (Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10023718mg PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 5.3e-35
Identity = 64/128 (50.00%), Postives = 86/128 (67.19%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G    NC +  S ++AS    V  YLGRPWKEYS  V++++F+DS++ P GW  
Sbjct: 412 DPNQNTGTSIHNCTIRASNDLASGSQTVQTYLGRPWKEYSRTVYMQTFMDSLINPAGWHD 471

Query: 152 WP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  +  L++AEYNNTGPG+DT+ RV WPGYHI+  AT+   FTV NF+ G  WLP+T
Sbjct: 472 WSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHII-DATEAANFTVSNFLSGDNWLPQT 531

Query: 212 GIPFRTGF 219
           G+P+  GF
Sbjct: 532 GVPYNGGF 538

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR10
Match: AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 139.0 bits (349), Expect = 3.4e-33
Identity = 57/127 (44.88%), Postives = 83/127 (65.35%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G     C +  + ++A+S   V  YLGRPWKEYS  V +++++D  ++P GW  
Sbjct: 432 DPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNA 491

Query: 152 WP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  +  L++AEYNNTGPG+DT+ RV WPGYH+++ AT  + FTV NF+ G  W+ +T
Sbjct: 492 WSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVIN-ATDASNFTVTNFLVGEGWIGQT 551

Query: 212 GIPFRTG 218
           G+PF  G
Sbjct: 552 GVPFVGG 557

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 138.3 bits (347), Expect = 5.8e-33
Identity = 58/127 (45.67%), Postives = 84/127 (66.14%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G    NC +  + ++ SS   V  YLGRPWKEYS  VF++S++D VV+P GW  
Sbjct: 445 DPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWRE 504

Query: 152 W-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  +  L++AEYNNTG G+ T+ RV WPGYH+++ +T    FTVENF+ G  W+ ++
Sbjct: 505 WNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVIN-STDANNFTVENFLLGDGWMVQS 564

Query: 212 GIPFRTG 218
           G+P+ +G
Sbjct: 565 GVPYISG 570

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 134.0 bits (336), Expect = 1.1e-31
Identity = 57/129 (44.19%), Postives = 84/129 (65.12%), Query Frame = 1


Query: 92  DRNDQ-SGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWL 151
           +++DQ +G    NC++  +P++   K+    YLGRPW++YS  V I+SF+D ++ P GWL
Sbjct: 404 NQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWL 463

Query: 152 -GWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPE 211
            G  D  ++ L++ EY N GPGA+ ++RV WPG+  +   T+ T+FTV  FI GS WL  
Sbjct: 464 EGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNS 523

Query: 212 TGIPFRTGF 219
           TGIPF  GF
Sbjct: 524 TGIPFSLGF 532

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR10
Match: AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 132.9 bits (333), Expect = 2.4e-31
Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G   QNC +  + ++ SS   V  YLGRPWKEYS  V+++S++D  V+P GW  
Sbjct: 451 DPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWRE 510

Query: 152 W-PDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  +  L++AEYNNTGPG++T+ RV WPGYH+++ +T    FTV      + W+ +T
Sbjct: 511 WNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVIN-STDAANFTVTGLFIEADWIWKT 570

Query: 212 GIPFRTG 218
           G+P+ +G
Sbjct: 571 GVPYTSG 576

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR10
Match: AT5G04970.1 (AT5G04970.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 132.5 bits (332), Expect = 3.2e-31
Identity = 58/127 (45.67%), Postives = 79/127 (62.20%), Query Frame = 1


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N ++G    NC +  +P++A+     + +LGRPWK YS  V+I+S++  VVQP GWL 
Sbjct: 494 DPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 553

Query: 152 WPDVP-VDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W     +D + + EY+N GPGADTS+RV W GY +L+   Q   FTV NF  G  WLP+T
Sbjct: 554 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLN-LVQAMNFTVYNFTLGDTWLPQT 613

Query: 212 GIPFRTG 218
            IPF  G
Sbjct: 614 DIPFYGG 619

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: gi|778697621|ref|XP_011654361.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 206.8 bits (525), Expect = 3.7e-50
Identity = 90/125 (72.00%), Postives = 107/125 (85.60%), Query Frame = 1


Query: 94  NDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWP 153
           N  SGF+FQNCKVTVSPEIASSKDKV ++LGRPW+ YS VVFI+SFLD+VVQPKGWL WP
Sbjct: 213 NSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWP 272

Query: 154 DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIP 213
            VP + L++AEYNN+G GA+TS+RV+WPGYH L+ A +V  FTVE FI G+QWLP+TGIP
Sbjct: 273 GVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVEIFINGTQWLPQTGIP 332

Query: 214 FRTGF 219
           FR GF
Sbjct: 333 FRAGF 337

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: gi|700198848|gb|KGN54006.1| (hypothetical protein Csa_4G260430 [Cucumis sativus])

HSP 1 Score: 206.8 bits (525), Expect = 3.7e-50
Identity = 90/125 (72.00%), Postives = 107/125 (85.60%), Query Frame = 1


Query: 94  NDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWP 153
           N  SGF+FQNCKVTVSPEIASSKDKV ++LGRPW+ YS VVFI+SFLD+VVQPKGWL WP
Sbjct: 206 NSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWP 265

Query: 154 DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIP 213
            VP + L++AEYNN+G GA+TS+RV+WPGYH L+ A +V  FTVE FI G+QWLP+TGIP
Sbjct: 266 GVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVEIFINGTQWLPQTGIP 325

Query: 214 FRTGF 219
           FR GF
Sbjct: 326 FRAGF 330

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: gi|659131731|ref|XP_008465826.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 204.1 bits (518), Expect = 2.4e-49
Identity = 88/122 (72.13%), Postives = 105/122 (86.07%), Query Frame = 1


Query: 97  SGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWPDVP 156
           SGF+FQNC VTVSPEIASSKDKV ++LGRPW+ YS VVFI+SFLDSVVQPKGWL WP VP
Sbjct: 203 SGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEWPGVP 262

Query: 157 VDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIPFRT 216
            + L++AEYNN+G GA+TS+RV+WPGYH+L  A +V  F+VE FI G+QWLP+TGIPFR 
Sbjct: 263 ENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGIPFRA 322

Query: 217 GF 219
           GF
Sbjct: 323 GF 324

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: gi|778708551|ref|XP_011656233.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 202.2 bits (513), Expect = 9.2e-49
Identity = 87/125 (69.60%), Postives = 104/125 (83.20%), Query Frame = 1


Query: 94  NDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWP 153
           N  SGF+FQNC VTVSPEIA SKD V ++LGRPW+ YS VVFI+SFLD+VVQPKGWL WP
Sbjct: 213 NSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWP 272

Query: 154 DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIP 213
            VP + L++AEYNN+G GA+TS+RV+WPGYH L+ A +V  FTVE FI G+QWLP+TGIP
Sbjct: 273 GVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGIP 332

Query: 214 FRTGF 219
           FR GF
Sbjct: 333 FRAGF 337

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: gi|700196022|gb|KGN51199.1| (hypothetical protein Csa_5G488750 [Cucumis sativus])

HSP 1 Score: 202.2 bits (513), Expect = 9.2e-49
Identity = 87/125 (69.60%), Postives = 104/125 (83.20%), Query Frame = 1


Query: 94  NDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWP 153
           N  SGF+FQNC VTVSPEIA SKD V ++LGRPW+ YS VVFI+SFLD+VVQPKGWL WP
Sbjct: 44  NSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWP 103

Query: 154 DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIP 213
            VP + L++AEYNN+G GA+TS+RV+WPGYH L+ A +V  FTVE FI G+QWLP+TGIP
Sbjct: 104 GVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGIP 163

Query: 214 FRTGF 219
           FR GF
Sbjct: 164 FRAGF 168

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g07420 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME2_CITSI	1.2e-35	47.24	Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1	[more]
PME20_ARATH	6.0e-32	44.88	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=P...	[more]
PME41_ARATH	1.0e-31	45.67	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=P...	[more]
PME39_ARATH	1.9e-30	44.19	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=P...	[more]
PME7_ARATH	4.3e-30	43.31	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PM...	[more]

BLAST of Cp4.1LG11g07420 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1M3_CUCSA	2.6e-50	72.00	Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1	[more]
A0A0A0KQN0_CUCSA	6.4e-49	69.60	Pectinesterase OS=Cucumis sativus GN=Csa_5G488750 PE=4 SV=1	[more]
A0A0A0LR14_CUCSA	1.4e-40	62.81	Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1	[more]
A0A067H5N1_CITSI	5.3e-35	50.00	Pectinesterase OS=Citrus sinensis GN=CISIN_1g043374mg PE=4 SV=1	[more]
V4TBH6_9ROSI	5.3e-35	50.00	Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10023718mg PE=4 SV=1	[more]

BLAST of Cp4.1LG11g07420 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G47550.1	3.4e-33	44.88	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	5.8e-33	45.67	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	1.1e-31	44.19	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	2.4e-31	43.31	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G04970.1	3.2e-31	45.67	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

BLAST of Cp4.1LG11g07420 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778697621\|ref\|XP_011654361.1\|	3.7e-50	72.00	PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus]	[more]
gi\|700198848\|gb\|KGN54006.1\|	3.7e-50	72.00	hypothetical protein Csa_4G260430 [Cucumis sativus]	[more]
gi\|659131731\|ref\|XP_008465826.1\|	2.4e-49	72.13	PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo]	[more]
gi\|778708551\|ref\|XP_011656233.1\|	9.2e-49	69.60	PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus]	[more]
gi\|700196022\|gb\|KGN51199.1\|	9.2e-49	69.60	hypothetical protein Csa_5G488750 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **INTERPRO**
Term	Definition
IPR018040	Pectinesterase_Tyr_AS
IPR012334	Pectin_lyas_fold
IPR011050	Pectin_lyase_fold/virulence
IPR000070	Pectinesterase_cat

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005618	cell wall
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g07420.1	Cp4.1LG11g07420.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 92..203 score: 7.8
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 21..216 score: 3.92
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 22..93 score: 4.6E-18coord: 94..215 score: 3.9
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 51..70 scor
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 26..218 score: 6.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG11g07420	CmaCh12G007210	Cucurbita maxima (Rimu)	cmacpeB181
Cp4.1LG11g07420	CmoCh12G006530	Cucurbita moschata (Rifu)	cmocpeB155
Cp4.1LG11g07420	CmoCh05G009280	Cucurbita moschata (Rifu)	cmocpeB721
Cp4.1LG11g07420	Csa5G488750	Cucumber (Chinese Long) v2	cpecuB117
Cp4.1LG11g07420	CSPI05G16600	Wild cucumber (PI 183967)	cpecpiB115
Cp4.1LG11g07420	MELO3C002915.2	Melon (DHL92) v3.6.1	cpemedB100
Cp4.1LG11g07420	CsaV3_5G025660	Cucumber (Chinese Long) v3	cpecucB0129
Cp4.1LG11g07420	Bhi06G000656	Wax gourd	cpewgoB0129
Cp4.1LG11g07420	CsGy5G016420	Cucumber (Gy14) v2	cgybcpeB593

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG11g07420	Cp4.1LG07g05500	Cucurbita pepo (Zucchini)	cpecpeB149

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG11g07420	Cucumber (Gy14) v1	cgycpeB0723
Cp4.1LG11g07420	Cucurbita maxima (Rimu)	cmacpeB767
Cp4.1LG11g07420	Cucurbita moschata (Rifu)	cmocpeB726
Cp4.1LG11g07420	Bottle gourd (USVL1VR-Ls)	cpelsiB084
Cp4.1LG11g07420	Watermelon (Charleston Gray)	cpewcgB123
Cp4.1LG11g07420	Melon (DHL92) v3.5.1	cpemeB078
Cp4.1LG11g07420	Silver-seed gourd	carcpeB0191
Cp4.1LG11g07420	Silver-seed gourd	carcpeB1477