CmaCh19G004710 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh19G004710
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Cystatin domain containing protein
Location	Cma_Chr19 : 5639964 .. 5640335 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTCTACCATGCTCGCTAACATCGGTCAACACGATCCCATCCTCCATGTCACAAAGGATGCGAAGGGCGACCATCGTTCTGAATGGATATCGATTCGCGCTATTGAGGACCCCTATGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTGGAGGTAAAAGGAGGTGCTTCTGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

mRNA sequence

ATGATTTCTACCATGCTCGCTAACATCGGTCAACACGATCCCATCCTCCATGTCACAAAGGATGCGAAGGGCGACCATCGTTCTGAATGGATATCGATTCGCGCTATTGAGGACCCCTATGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTGGAGGTAAAAGGAGGTGCTTCTGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

Coding sequence (CDS)

ATGATTTCTACCATGCTCGCTAACATCGGTCAACACGATCCCATCCTCCATGTCACAAAGGATGCGAAGGGCGACCATCGTTCTGAATGGATATCGATTCGCGCTATTGAGGACCCCTATGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTGGAGGTAAAAGGAGGTGCTTCTGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

Protein sequence

MISTMLANIGQHDPILHVTKDAKGDHRSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGVE

Homology

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BLAST of CmaCh19G004710 vs. Swiss-Prot
Match: CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.9e-07
Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 1


Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I ++    VQ+VA+F V E NK+     QY+ ++R +  + + G  YRL++  K GA
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYT-QVVAGTNYRLVIAAKDGA 91

Query: 90  SEGKYEAIVWE 101
             G YEA+VW+
Sbjct: 92  VVGNYEAVVWD 101

[Close]

BLAST of CmaCh19G004710 vs. TrEMBL
Match: A0A0A0K644_CUCSA (Cystatin domain containing protein OS=Cucumis sativus GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 3.7e-46
Identity = 96/123 (78.05%), Postives = 105/123 (85.37%), Query Frame = 1


Query: 1   MISTMLANIGQHDPILHVT-KDAKGDHRSEWISIRAIEDPYVQEVAKFVVVEVNKEKKAT 60
           MIST+LAN+GQHDPILHVT KD KGDH+SEWISIRAIEDPYVQEVAKF + E  KEK A 
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKGLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVAC 120
           FQYK LL CW+KEEMGGIKYRL+LEV K    EGKYEA+VWEASGRLIIRKLKL+SF+  
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 NHG 122
            HG
Sbjct: 121 MHG 123

[Close]

BLAST of CmaCh19G004710 vs. TrEMBL
Match: A0A0B4ZWL9_MORAL (Cysteine proteinase inhibitor OS=Morus alba var. atropurpurea PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.1e-08
Identity = 30/71 (42.25%), Postives = 47/71 (66.20%), Query Frame = 1


Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL+L VK GA
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVLAVKNGA 88

Query: 90  SEGKYEAIVWE 101
           +  +YEA+VWE
Sbjct: 89  TAERYEAVVWE 98

[Close]

BLAST of CmaCh19G004710 vs. TrEMBL
Match: W9R7F6_9ROSA (Cysteine proteinase inhibitor OS=Morus notabilis GN=L484_004273 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.4e-07
Identity = 28/71 (39.44%), Postives = 46/71 (64.79%), Query Frame = 1


Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL++ V  GA
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVVAVTNGA 88

Query: 90  SEGKYEAIVWE 101
           +  +YEA+VWE
Sbjct: 89  AAERYEAVVWE 98

[Close]

BLAST of CmaCh19G004710 vs. TrEMBL
Match: A0A0D2VC84_GOSRA (Cysteine proteinase inhibitor OS=Gossypium raimondii GN=B456_010G187500 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.0e-06
Identity = 28/71 (39.44%), Postives = 46/71 (64.79%), Query Frame = 1


Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ I+DP+V E+AKF V E NK+ K + +   +++    + + GI Y+L+L+ K G+
Sbjct: 33  WTPIKDIKDPHVMEIAKFAVDEYNKQSKVSLKLDEVVK-GETQVVSGINYKLVLKAKDGS 92

Query: 90  SEGKYEAIVWE 101
           +   YEA+VWE
Sbjct: 93  AVNTYEAVVWE 102

[Close]

BLAST of CmaCh19G004710 vs. TrEMBL
Match: A0A0A0LTF5_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_1G183580 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.0e-06
Identity = 31/79 (39.24%), Postives = 46/79 (58.23%), Query Frame = 1


Query: 23  KGDHRSEWISIRAIEDPYVQEVAKFVVVEVNKEKKAT-FQYKGLLRCWVKEEMGGIKYRL 82
           KG     W  I  ++DP+V+E+ KF V E NK+ K    ++K ++     + + G  YRL
Sbjct: 22  KGSMLGGWSKIPDLKDPHVEEIGKFAVAEYNKQSKGVAIEFKSVVS-GETQVVAGTNYRL 81

Query: 83  ILEVKGGASEGKYEAIVWE 101
           +++ K G S  KYEAIVWE
Sbjct: 82  LIDAKRGESMSKYEAIVWE 99

[Close]

BLAST of CmaCh19G004710 vs. NCBI nr
Match: gi|700188534|gb|KGN43767.1| (Cystatin domain containing protein [Cucumis sativus])

HSP 1 Score: 192.2 bits (487), Expect = 5.4e-46
Identity = 96/123 (78.05%), Postives = 105/123 (85.37%), Query Frame = 1


Query: 1   MISTMLANIGQHDPILHVT-KDAKGDHRSEWISIRAIEDPYVQEVAKFVVVEVNKEKKAT 60
           MIST+LAN+GQHDPILHVT KD KGDH+SEWISIRAIEDPYVQEVAKF + E  KEK A 
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKGLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVAC 120
           FQYK LL CW+KEEMGGIKYRL+LEV K    EGKYEA+VWEASGRLIIRKLKL+SF+  
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 NHG 122
            HG
Sbjct: 121 MHG 123

[Close]

BLAST of CmaCh19G004710 vs. NCBI nr
Match: gi|746657137|gb|AJD79055.1| (CPI-4 [Morus alba var. atropurpurea])

HSP 1 Score: 66.2 bits (160), Expect = 4.4e-08
Identity = 30/71 (42.25%), Postives = 47/71 (66.20%), Query Frame = 1


Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL+L VK GA
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVLAVKNGA 88

Query: 90  SEGKYEAIVWE 101
           +  +YEA+VWE
Sbjct: 89  TAERYEAVVWE 98

[Close]

BLAST of CmaCh19G004710 vs. NCBI nr
Match: gi|703092669|ref|XP_010094696.1| (Cysteine proteinase inhibitor 5 [Morus notabilis])

HSP 1 Score: 62.8 bits (151), Expect = 4.9e-07
Identity = 28/71 (39.44%), Postives = 46/71 (64.79%), Query Frame = 1


Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL++ V  GA
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVVAVTNGA 88

Query: 90  SEGKYEAIVWE 101
           +  +YEA+VWE
Sbjct: 89  AAERYEAVVWE 98

[Close]

BLAST of CmaCh19G004710 vs. NCBI nr
Match: gi|659127356|ref|XP_008463660.1| (PREDICTED: cysteine proteinase inhibitor 5 [Cucumis melo])

HSP 1 Score: 62.4 bits (150), Expect = 6.4e-07
Identity = 29/72 (40.28%), Postives = 46/72 (63.89%), Query Frame = 1


Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKAT-FQYKGLLRCWVKEEMGGIKYRLILEVKGG 89
           W  I  ++DP+V+E+AKF V + N++ K    ++KG+      + + G  YRL+++ K G
Sbjct: 32  WSKISDVKDPHVEEIAKFAVAQYNEQSKGVAIEFKGV-ESGETQVVAGTNYRLVIDAKRG 91

Query: 90  ASEGKYEAIVWE 101
            S GKYEA+VWE
Sbjct: 92  ESIGKYEALVWE 102

[Close]

BLAST of CmaCh19G004710 vs. NCBI nr
Match: gi|823138315|ref|XP_012469025.1| (PREDICTED: cysteine proteinase inhibitor 5-like, partial [Gossypium raimondii])

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-06
Identity = 34/99 (34.34%), Postives = 59/99 (59.60%), Query Frame = 1


Query: 18  VTKDAKGDHRSE-WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGG 77
           +  DA+ D   E W SI+ I+DP+V E+ +F V E N + K + + + +++    + + G
Sbjct: 5   ILSDARKDALIEGWTSIKNIKDPHVTEITEFAVDEHNTQLKGSLKLEEVVK-GETQVISG 64

Query: 78  IKYRLILEVKGGASEGKYEAIVWEASGRLIIRKLKLVSF 116
           I Y+L+L+ K G ++  YEA+VWE   +  ++  KL SF
Sbjct: 65  INYKLVLQAKDGTADNSYEAVVWE---KAWLKFRKLTSF 99

The following BLAST results are available for this feature:

BLAST of CmaCh19G004710 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYT1_ACTDE	2.9e-07	38.03	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1	[more]

BLAST of CmaCh19G004710 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K644_CUCSA	3.7e-46	78.05	Cystatin domain containing protein OS=Cucumis sativus GN=Csa_7G066240 PE=4 SV=1	[more]
A0A0B4ZWL9_MORAL	3.1e-08	42.25	Cysteine proteinase inhibitor OS=Morus alba var. atropurpurea PE=2 SV=1	[more]
W9R7F6_9ROSA	3.4e-07	39.44	Cysteine proteinase inhibitor OS=Morus notabilis GN=L484_004273 PE=3 SV=1	[more]
A0A0D2VC84_GOSRA	1.0e-06	39.44	Cysteine proteinase inhibitor OS=Gossypium raimondii GN=B456_010G187500 PE=3 SV=...	[more]
A0A0A0LTF5_CUCSA	1.0e-06	39.24	Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_1G183580 PE=3 SV=1	[more]

BLAST of CmaCh19G004710 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmaCh19G004710 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700188534\|gb\|KGN43767.1\|	5.4e-46	78.05	Cystatin domain containing protein [Cucumis sativus]	[more]
gi\|746657137\|gb\|AJD79055.1\|	4.4e-08	42.25	CPI-4 [Morus alba var. atropurpurea]	[more]
gi\|703092669\|ref\|XP_010094696.1\|	4.9e-07	39.44	Cysteine proteinase inhibitor 5 [Morus notabilis]	[more]
gi\|659127356\|ref\|XP_008463660.1\|	6.4e-07	40.28	PREDICTED: cysteine proteinase inhibitor 5 [Cucumis melo]	[more]
gi\|823138315\|ref\|XP_012469025.1\|	1.1e-06	34.34	PREDICTED: cysteine proteinase inhibitor 5-like, partial [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR020381	Proteinase inhibitor I25, cystatin, conserved region

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh19G004710.1	CmaCh19G004710.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 33..102 score: 3.7
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 34..101 score: 1.
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 28..115 score: 3.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh19G004710	Cucsa.218360	Cucumber (Gy14) v1	cgycmaB0626
CmaCh19G004710	Cla012895	Watermelon (97103) v1	cmawmB473
CmaCh19G004710	Csa7G066240	Cucumber (Chinese Long) v2	cmacuB535
CmaCh19G004710	MELO3C018593	Melon (DHL92) v3.5.1	cmameB457
CmaCh19G004710	ClCG09G015860	Watermelon (Charleston Gray)	cmawcgB442
CmaCh19G004710	CSPI07G06040	Wild cucumber (PI 183967)	cmacpiB540
CmaCh19G004710	CmoCh11G014750	Cucurbita moschata (Rifu)	cmacmoB494
CmaCh19G004710	Lsi03G007240	Bottle gourd (USVL1VR-Ls)	cmalsiB484
CmaCh19G004710	Cp4.1LG15g03650	Cucurbita pepo (Zucchini)	cmacpeB522
CmaCh19G004710	MELO3C018593.2	Melon (DHL92) v3.6.1	cmamedB530
CmaCh19G004710	CsaV3_7G007000	Cucumber (Chinese Long) v3	cmacucB0644
CmaCh19G004710	Bhi05G000747	Wax gourd	cmawgoB0642
CmaCh19G004710	CsGy7G005700	Cucumber (Gy14) v2	cgybcmaB951
CmaCh19G004710	Carg22817	Silver-seed gourd	carcmaB0401

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh19G004710	Cucurbita pepo (Zucchini)	cmacpeB539
CmaCh19G004710	Bottle gourd (USVL1VR-Ls)	cmalsiB483
CmaCh19G004710	Cucumber (Gy14) v2	cgybcmaB945
CmaCh19G004710	Melon (DHL92) v3.6.1	cmamedB535
CmaCh19G004710	Silver-seed gourd	carcmaB0528
CmaCh19G004710	Cucumber (Chinese Long) v3	cmacucB0639
CmaCh19G004710	Watermelon (97103) v2	cmawmbB524
CmaCh19G004710	Watermelon (97103) v2	cmawmbB550
CmaCh19G004710	Watermelon (97103) v2	cmawmbB558
CmaCh19G004710	Wax gourd	cmawgoB0634
CmaCh19G004710	Cucurbita maxima (Rimu)	cmacmaB149
CmaCh19G004710	Cucurbita maxima (Rimu)	cmacmaB342
CmaCh19G004710	Cucumber (Gy14) v1	cgycmaB0624
CmaCh19G004710	Cucurbita moschata (Rifu)	cmacmoB508
CmaCh19G004710	Cucurbita moschata (Rifu)	cmacmoB530
CmaCh19G004710	Wild cucumber (PI 183967)	cmacpiB534
CmaCh19G004710	Cucumber (Chinese Long) v2	cmacuB528
CmaCh19G004710	Melon (DHL92) v3.5.1	cmameB463
CmaCh19G004710	Watermelon (Charleston Gray)	cmawcgB450
CmaCh19G004710	Watermelon (Charleston Gray)	cmawcgB454