ClCG09G015860 (gene) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG09G015860
Type	gene
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	Cysteine proteinase inhibitor
Location	CG_Chr09 : 30822231 .. 30822608 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTCTACCGTGCTCGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGAATACGACGAAGGGCGATGAACAGTCCGAATGGATATCGATTCGTGCTATTGAAGATCCTTACGTGCAAGAAGTTGCAAAGTTTGCAGTGACAGAGTTTGATAAGGAAAAAATGGCAAAGTTTCAATACAAGCACTTGCTACGTTGTTGGATTAAGGAGGAAATGGGAGGCATAAAATATCGCCTTATATTAGAGGTAACTAAAGAAGGAAAAGTTGAGGGTAAATATGAAGCTATTGTATGGGAAGCTTCTGGCCGTCTGATTATAAGGAAGCTTAAGCTTATATCTTTTATGCCTTGTATTCATGGGGCTGAATGA

mRNA sequence

ATGATTTCTACCGTGCTCGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGAATACGACGAAGGGCGATGAACAGTCCGAATGGATATCGATTCGTGCTATTGAAGATCCTTACGTGCAAGAAGTTGCAAAGTTTGCAGTGACAGAGTTTGATAAGGAAAAAATGGCAAAGTTTCAATACAAGCACTTGCTACGTTGTTGGATTAAGGAGGAAATGGGAGGCATAAAATATCGCCTTATATTAGAGGTAACTAAAGAAGGAAAAGTTGAGGGTAAATATGAAGCTATTGTATGGGAAGCTTCTGGCCGTCTGATTATAAGGAAGCTTAAGCTTATATCTTTTATGCCTTGTATTCATGGGGCTGAATGA

Coding sequence (CDS)

ATGATTTCTACCGTGCTCGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGAATACGACGAAGGGCGATGAACAGTCCGAATGGATATCGATTCGTGCTATTGAAGATCCTTACGTGCAAGAAGTTGCAAAGTTTGCAGTGACAGAGTTTGATAAGGAAAAAATGGCAAAGTTTCAATACAAGCACTTGCTACGTTGTTGGATTAAGGAGGAAATGGGAGGCATAAAATATCGCCTTATATTAGAGGTAACTAAAGAAGGAAAAGTTGAGGGTAAATATGAAGCTATTGTATGGGAAGCTTCTGGCCGTCTGATTATAAGGAAGCTTAAGCTTATATCTTTTATGCCTTGTATTCATGGGGCTGAATGA

Protein sequence

MISTVLANIGQHDPILHVTKNTTKGDEQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVTKEGKVEGKYEAIVWEASGRLIIRKLKLISFMPCIHGAE

Homology

[Close]

BLAST of ClCG09G015860 vs. Swiss-Prot
Match: CYT7_ORYSJ (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 8.5e-07
Identity = 32/83 (38.55%), Postives = 46/83 (55.42%), Query Frame = 1


Query: 37  IEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVTKEGKVEGKY 96
           I DP+VQE+ ++AV E D+   A       +    K+ + G+ YRL LE +  G  +G+Y
Sbjct: 35  INDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGRY 94

Query: 97  EAIVWEASGRLIIRKLKLISFMP 120
           EA+V+E   R   R  KL+SF P
Sbjct: 95  EAVVYEQDPRSNAR--KLVSFEP 115

[Close]

BLAST of ClCG09G015860 vs. Swiss-Prot
Match: CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 8.5e-07
Identity = 27/72 (37.50%), Postives = 44/72 (61.11%), Query Frame = 1


Query: 31  WISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVTKEG 90
           W  I ++    VQ+VA+FAV+E +K+   + QY+ ++R +  + + G  YRL++   K+G
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGY-TQVVAGTNYRLVI-AAKDG 91

Query: 91  KVEGKYEAIVWE 103
            V G YEA+VW+
Sbjct: 92  AVVGNYEAVVWD 101

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BLAST of ClCG09G015860 vs. TrEMBL
Match: A0A0A0K644_CUCSA (Cystatin domain containing protein OS=Cucumis sativus GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 2.2e-54
Identity = 105/124 (84.68%), Postives = 114/124 (91.94%), Query Frame = 1


Query: 1   MISTVLANIGQHDPILHVTKNTTKGDEQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAK 60
           MIST+LAN+GQHDPILHVT   TKGD QSEWISIRAIEDPYVQEVAKFA+ EF+KEKMA+
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKHLLRCWIKEEMGGIKYRLILEVTKEGKVEGKYEAIVWEASGRLIIRKLKLISFMPC 120
           FQYK LL CWIKEEMGGIKYRL+LEV KE K+EGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 IHGA 125
           +HGA
Sbjct: 121 MHGA 124

[Close]

BLAST of ClCG09G015860 vs. TrEMBL
Match: A0A0B4ZWL9_MORAL (Cysteine proteinase inhibitor OS=Morus alba var. atropurpurea PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 6.6e-06
Identity = 28/72 (38.89%), Postives = 46/72 (63.89%), Query Frame = 1


Query: 31  WISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVTKEG 90
           W  I+ + DP+V+E+A FAV+E++K+  A  + K +++    + + G  YRL+L V K G
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVLAV-KNG 88

Query: 91  KVEGKYEAIVWE 103
               +YEA+VWE
Sbjct: 89  ATAERYEAVVWE 98

[Close]

BLAST of ClCG09G015860 vs. TrEMBL
Match: A0A0D2SVE6_GOSRA (Cysteine proteinase inhibitor OS=Gossypium raimondii GN=B456_010G187300 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 8.6e-06
Identity = 31/87 (35.63%), Postives = 51/87 (58.62%), Query Frame = 1


Query: 18  VTKNTTKGDEQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGG 77
           +  +  KG     W  I+ I+DP+V E+A+FAV E++K   +    + +++    + + G
Sbjct: 20  IFSDAKKGPIIGGWTRIKDIKDPHVTEIAEFAVNEYNKLSSSSLVLQKVVK-GETQVVAG 79

Query: 78  IKYRLILEVTKEGKVEGKYEAIVWEAS 105
           I YRL+L+  K G V  KY+A+VWE S
Sbjct: 80  INYRLVLK-AKNGSVAKKYQAVVWEKS 104

[Close]

BLAST of ClCG09G015860 vs. NCBI nr
Match: gi|700188534|gb|KGN43767.1| (Cystatin domain containing protein [Cucumis sativus])

HSP 1 Score: 219.5 bits (558), Expect = 3.2e-54
Identity = 105/124 (84.68%), Postives = 114/124 (91.94%), Query Frame = 1


Query: 1   MISTVLANIGQHDPILHVTKNTTKGDEQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAK 60
           MIST+LAN+GQHDPILHVT   TKGD QSEWISIRAIEDPYVQEVAKFA+ EF+KEKMA+
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKHLLRCWIKEEMGGIKYRLILEVTKEGKVEGKYEAIVWEASGRLIIRKLKLISFMPC 120
           FQYK LL CWIKEEMGGIKYRL+LEV KE K+EGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 IHGA 125
           +HGA
Sbjct: 121 MHGA 124

[Close]

BLAST of ClCG09G015860 vs. NCBI nr
Match: gi|502184098|ref|XP_004517283.1| (PREDICTED: cysteine proteinase inhibitor 5-like [Cicer arietinum])

HSP 1 Score: 62.8 bits (151), Expect = 5.0e-07
Identity = 37/104 (35.58%), Postives = 57/104 (54.81%), Query Frame = 1


Query: 15  ILHVTKNTTKGDEQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEE 74
           +L  +K T        W  I  I DP+V +VA FAVTE+DK   AK +++ +++    + 
Sbjct: 12  VLLASKTTRNEAIPGGWSPIENINDPHVTDVANFAVTEYDKRSGAKLKFEKVIK-GESQV 71

Query: 75  MGGIKYRLILEVTKEGKVEGKYEAIVWEASGRLIIRKLKLISFM 119
           + G  YRL+L    +G V   YEA+VWE   ++ +R   L SF+
Sbjct: 72  VSGANYRLVLS-ANDGSVSNHYEAVVWE---KVWLRFRNLTSFV 110

[Close]

BLAST of ClCG09G015860 vs. NCBI nr
Match: gi|746657137|gb|AJD79055.1| (CPI-4 [Morus alba var. atropurpurea])

HSP 1 Score: 58.5 bits (140), Expect = 9.4e-06
Identity = 28/72 (38.89%), Postives = 46/72 (63.89%), Query Frame = 1


Query: 31  WISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVTKEG 90
           W  I+ + DP+V+E+A FAV+E++K+  A  + K +++    + + G  YRL+L V K G
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVLAV-KNG 88

Query: 91  KVEGKYEAIVWE 103
               +YEA+VWE
Sbjct: 89  ATAERYEAVVWE 98

The following BLAST results are available for this feature:

BLAST of ClCG09G015860 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYT7_ORYSJ	8.5e-07	38.55	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica GN=Os03...	[more]
CYT1_ACTDE	8.5e-07	37.50	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1	[more]

BLAST of ClCG09G015860 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K644_CUCSA	2.2e-54	84.68	Cystatin domain containing protein OS=Cucumis sativus GN=Csa_7G066240 PE=4 SV=1	[more]
A0A0B4ZWL9_MORAL	6.6e-06	38.89	Cysteine proteinase inhibitor OS=Morus alba var. atropurpurea PE=2 SV=1	[more]
A0A0D2SVE6_GOSRA	8.6e-06	35.63	Cysteine proteinase inhibitor OS=Gossypium raimondii GN=B456_010G187300 PE=3 SV=...	[more]

BLAST of ClCG09G015860 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700188534\|gb\|KGN43767.1\|	3.2e-54	84.68	Cystatin domain containing protein [Cucumis sativus]	[more]
gi\|502184098\|ref\|XP_004517283.1\|	5.0e-07	35.58	PREDICTED: cysteine proteinase inhibitor 5-like [Cicer arietinum]	[more]
gi\|746657137\|gb\|AJD79055.1\|	9.4e-06	38.89	CPI-4 [Morus alba var. atropurpurea]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR020381	Proteinase inhibitor I25, cystatin, conserved region

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0044464	cell part
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity
molecular_function	GO:0030414	peptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG09G015860.1	ClCG09G015860.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PRODOM	PD001231		coord: 42..102 score: 8.
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 34..117 score: 3.4
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 35..103 score: 1.
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 31..119 score: 1.08

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
ClCG09G015860	Csa7G066240	Cucumber (Chinese Long) v2	cuwcgB514
ClCG09G015860	MELO3C018593	Melon (DHL92) v3.5.1	mewcgB168
ClCG09G015860	Cla012895	Watermelon (97103) v1	wcgwmB009
ClCG09G015860	Bhi05G000747	Wax gourd	wcgwgoB033
ClCG09G015860	CSPI07G06040	Wild cucumber (PI 183967)	cpiwcgB557
ClCG09G015860	Cucsa.218360	Cucumber (Gy14) v1	cgywcgB391
ClCG09G015860	CmaCh19G004710	Cucurbita maxima (Rimu)	cmawcgB442
ClCG09G015860	CmoCh11G014750	Cucurbita moschata (Rifu)	cmowcgB072
ClCG09G015860	Lsi03G007240	Bottle gourd (USVL1VR-Ls)	lsiwcgB215
ClCG09G015860	Cp4.1LG15g03650	Cucurbita pepo (Zucchini)	cpewcgB224
ClCG09G015860	CsGy7G005700	Cucumber (Gy14) v2	cgybwcgB473
ClCG09G015860	MELO3C018593.2	Melon (DHL92) v3.6.1	medwcgB159
ClCG09G015860	Carg22817	Silver-seed gourd	carwcgB0280
ClCG09G015860	CsaV3_7G007000	Cucumber (Chinese Long) v3	cucwcgB535

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
ClCG09G015860	Silver-seed gourd	carwcgB0281
ClCG09G015860	Silver-seed gourd	carwcgB0363
ClCG09G015860	Watermelon (97103) v2	wcgwmbB035
ClCG09G015860	Wax gourd	wcgwgoB050
ClCG09G015860	Cucurbita maxima (Rimu)	cmawcgB075
ClCG09G015860	Cucurbita maxima (Rimu)	cmawcgB450
ClCG09G015860	Cucurbita moschata (Rifu)	cmowcgB441
ClCG09G015860	Cucurbita moschata (Rifu)	cmowcgB450
ClCG09G015860	Wild cucumber (PI 183967)	cpiwcgB541
ClCG09G015860	Melon (DHL92) v3.5.1	mewcgB176
ClCG09G015860	Cucurbita pepo (Zucchini)	cpewcgB218
ClCG09G015860	Cucurbita pepo (Zucchini)	cpewcgB578
ClCG09G015860	Bottle gourd (USVL1VR-Ls)	lsiwcgB213
ClCG09G015860	Melon (DHL92) v3.6.1	medwcgB171