CmaCh04G010690 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh04G010690 vs. Swiss-Prot
Match: TRM1_THEON (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) GN=trm1 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.0e-34
Identity = 121/378 (32.01%), Postives = 180/378 (47.62%), Query Frame = 1

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 123
           F+    A  RD+ VLA     K+ GS RVLDAL   GIR +RY +E  A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 124 NRGVIVKNLSRVAEGFGETRRWM----------VTNCDANRVMSEFYLQKDFFDFIDVDS 183
              +IV N+     G  E    M          VTN DANR+M+E   Q  +FDF+D+D 
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNADANRLMNE---QFRYFDFVDLDP 146

Query: 184 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ A+K  G+L +T+TD     G + +  L  Y A        +E GLR+
Sbjct: 147 FGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYRNACLRKYLAEPIRGELCHEAGLRI 206

Query: 244 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQ 303
           LIG + R A+     V  L +YY  H   FR  L++  G    ++    + Y  +     
Sbjct: 207 LIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ----- 266

Query: 304 GFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKD 363
                E G+      +T +       GPLW GPL + +++E++  L+ +   +G+   K 
Sbjct: 267 ----DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS---KK 326

Query: 364 LEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNA 423
            +K LG + DE +  +P  Y     VA R  +    L  ++  + + GY A+R+H +  A
Sbjct: 327 TQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPTA 377

Query: 424 IKTNCPMAECIRIALELQ 432
           +KT+ P  E +    ELQ
Sbjct: 387 LKTDAPFEEVLEALRELQ 377

BLAST of CmaCh04G010690 vs. Swiss-Prot
Match: TRM1_PYRAB (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=trm1 PE=3 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 1.6e-31
Identity = 103/369 (27.91%), Postives = 175/369 (47.43%), Query Frame = 1

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 123
           F+    A  RD+ V+   + +       VLDAL   GIR +R+ +E  A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVVLLNVLKPRI----VLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 124 NRGVIVKNL-----SRVAEGFGET-----RRWMVTNCDANRVMSEFYLQKDFFDFIDVDS 183
              ++ KN+       + E  G       +  +V + DANR+M+E   +  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 184 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L +T+TDG    G HP   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 244 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNR---HYGFVSYCKKCG 303
           L+G VAR A+     +  + +YY  H   FR  +++  G    +    + G++ + +  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 304 NSQGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQ 363
                   EV +   P             GP+W GPL S + +E+ML ++ Q   +   +
Sbjct: 267 KF------EVERSFLPSKPNAY-------GPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 364 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 420
            K   KLL  + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

BLAST of CmaCh04G010690 vs. Swiss-Prot
Match: TRM1_PYRFU (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) GN=trm1 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.5e-31
Identity = 107/369 (29.00%), Postives = 172/369 (46.61%), Query Frame = 1

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 123
           F+    A  RD+ VL   + +       VLDAL   GIR +R+ +E  A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVLLLNVLKPKI----VLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 124 NRGVIVKNLSRVAEG----------FGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDS 183
              ++ KN+    +G          F   +  ++ N DANR+M+E   +  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 184 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L VT+TDG    G HP   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 244 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKN---RHYGFVSYCKKCG 303
           L+G +AR A+     +  L +YY  H   FR  +++  G    +    + G+V + +K G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 304 NSQGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQ 363
             +                + +S      GPLW GPL +   + +ML L +    + N  
Sbjct: 269 RFE-------------VEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 364 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 420
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

BLAST of CmaCh04G010690 vs. Swiss-Prot
Match: TRM1_SULSO (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=trm1 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 6.6e-30
Identity = 98/360 (27.22%), Postives = 182/360 (50.56%), Query Frame = 1

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEA-EADFVLANDAND 123
           F+  +    RDL V+  ++ +       ++DAL   GIR +RY VE+ +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPKI----IVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 124 ENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLR 183
               +I  N      G    +   + N DAN ++ E        D+ID+D FGS + F+ 
Sbjct: 95  NATSLIQINAKN--NGIENAK---IYNKDANALLYEVKS-----DYIDIDPFGSPAPFIL 154

Query: 184 PAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREA 243
            ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + REA
Sbjct: 155 SSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREA 214

Query: 244 SVLGFYVTPLFSYYSYHGPVFRVMLRISRGR----INKNRHYGFVSYCKKCGNSQGFSWS 303
           ++L   V PLFS+Y+ +   +R+ +R+  G      N N+H  +   C +CG      + 
Sbjct: 215 AILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG------FQ 274

Query: 304 EVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLL 363
              + +C        E   + GPL+ GPL+S ++++ ++ L   + ++ +     ++KLL
Sbjct: 275 TFVEENCKTKCPVCGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKLL 334

Query: 364 GQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLNTMMSEMIKAGYAASRSHIASNAIKTN 418
              V E +++    +  + ++AS+ K+++ PP+ +++  +      ASR+H A   I+T+
Sbjct: 335 N--VIEKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRTD 364

BLAST of CmaCh04G010690 vs. Swiss-Prot
Match: TRM1_THEKO (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) GN=trm1 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 6.6e-30
Identity = 107/366 (29.23%), Postives = 176/366 (48.09%), Query Frame = 1

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 123
           F+    +  RD+ VLA     K  G  RVLDAL   GIR +RY +E  A+ V  ND ++E
Sbjct: 27  FYNPVMSLNRDISVLAV----KVLGPKRVLDALSATGIRGIRYALETPAEEVWLNDISEE 86

Query: 124 NRGVIVKNLSRVAEG--FGETRR---W-----MVTNCDANRVMSEFYLQKDFFDFIDVDS 183
             G++ KN S   +G  + E  R   W     ++   DANR+M+E +    +FDF+D+D 
Sbjct: 87  AYGLMKKNASLNIDGELYEEGDRSYLWGEKLVVINKGDANRLMAENFR---YFDFLDLDP 146

Query: 184 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ +++ +G+L VT+TD     G + +  L  Y A     P  +E GLR+
Sbjct: 147 FGSPVEFLDTALRSVRRNGVLAVTATDTGVLCGAYRNACLRKYLAEPIRGPLCHEAGLRI 206

Query: 244 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQ 303
           LIG V R A+     V  L +Y+  H   FR  L++  G               K  +  
Sbjct: 207 LIGTVVRYAAKYDLGVEVLLAYHRDH--YFRAFLKLKSG----------AKKADKSLSQL 266

Query: 304 GFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKD 363
           GF W++            + +    SGPLW GPL    ++E++L  A     + + +   
Sbjct: 267 GFLWADKSG-RFEYRRGFLPDKPGASGPLWLGPLKDESFVEELLESARDHP-LAHKKTLS 326

Query: 364 LEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNA 420
             +L+ + +D     +P  Y     +A    +  P L+ ++  + + G++A+++H +  +
Sbjct: 327 FLQLISEELD-----VPFHY-DTHTLARACSLTPPKLDVIIQRLRELGHSATKTHFSPTS 365

BLAST of CmaCh04G010690 vs. TrEMBL
Match: A0A0A0KP99_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G220920 PE=4 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 1.8e-228
Identity = 395/441 (89.57%), Postives = 415/441 (94.10%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTV   TLSPSPFL   QT SH  +SS+IHLFQFS S K PLNNCL NNRTERG++FDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           AECIRIA +LQ++CLG HQVT
Sbjct: 421 AECIRIA-QLQRNCLGAHQVT 440

BLAST of CmaCh04G010690 vs. TrEMBL
Match: M5XJK8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005915mg PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 8.7e-170
Identity = 300/434 (69.12%), Postives = 354/434 (81.57%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPL--NNCLPNNRTERGIEF 60
           M+T+   TLSP  FL +        +S    + Q+S S  KP   +NC    +TERG++F
Sbjct: 1   MITLTPKTLSPFSFLCNPIPKHQNQNSKYTIVTQYSDSNSKPTFNSNCESEFQTERGLKF 60

Query: 61  DIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLAN 120
           DIG+TFFRHESATGRDLGVLAAALY++SKG LRVLDALCGCGIRSLRYL+EAEADFVLAN
Sbjct: 61  DIGETFFRHESATGRDLGVLAAALYKRSKGKLRVLDALCGCGIRSLRYLIEAEADFVLAN 120

Query: 121 DANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDS 180
           DAND+NR VI +NLS+V  G  +  RW VT  DANR+M+E YL++DFFD ID+DSFGSDS
Sbjct: 121 DANDDNRKVITENLSQVESG--DDPRWAVTLSDANRIMTECYLKRDFFDLIDIDSFGSDS 180

Query: 181 TFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGV 240
           +FLR AINALKLDGLLYVTSTDGFSSGG  P Q+LAAYGAYVRPMP+SNE+GLRMLIGG 
Sbjct: 181 SFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRMLIGGA 240

Query: 241 AREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWS 300
            R ASVLG+ VTPLFSYYSYHGP+FRVMLR++RG++  NR+YGF+SYC  CG+SQ +SW+
Sbjct: 241 VRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQEYSWA 300

Query: 301 EVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLL 360
           E+G+MSCPC+D++VS+SL+VSGPLWTGPLH   YI +ML LA +W W G+G G DL+KLL
Sbjct: 301 ELGRMSCPCSDSKVSKSLIVSGPLWTGPLHDTIYITEMLHLAEEWGWAGSGSGTDLDKLL 360

Query: 361 GQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNC 420
            QMV ESD +LP GY K+DEVASRAKVNSPPL  +MS + K GYAASRSHI SNAIKTNC
Sbjct: 361 NQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAIKTNC 420

Query: 421 PMAECIRIALELQQ 433
            MA CIRIA +LQQ
Sbjct: 421 SMAGCIRIAKQLQQ 432

BLAST of CmaCh04G010690 vs. TrEMBL
Match: B9I878_POPTR (N2 family protein OS=Populus trichocarpa GN=POPTR_0013s09180g PE=4 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.1e-168
Identity = 290/398 (72.86%), Postives = 335/398 (84.17%), Query Frame = 1

Query: 37  QSQKKPLNN--CLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLD 96
           Q  K  +N   C    ++ERG+EF+ G+TFFRHESA GRDLGVLAA+LY +SKG LRVLD
Sbjct: 24  QHYKPQINGIYCKSELQSERGLEFETGETFFRHESAKGRDLGVLAASLYNQSKGKLRVLD 83

Query: 97  ALCGCGIRSLRYLVEAEADFVLANDANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANR 156
           ALCGCGIRSLRYL+EA+ADFVLANDANDE+R VI++NL RV  GFG+ R+W+VT+ DANR
Sbjct: 84  ALCGCGIRSLRYLMEAKADFVLANDANDEHRRVILENLKRVDRGFGDERKWVVTHLDANR 143

Query: 157 VMSEFYLQKDFFDFIDVDSFGSD-STFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSL 216
           V+SE YLQKDFFD ID+DSFGSD S FLR A+N L  DGLLYVTSTDG SSGGHHP  SL
Sbjct: 144 VLSECYLQKDFFDLIDIDSFGSDISLFLRSAMNTLSFDGLLYVTSTDGHSSGGHHPDHSL 203

Query: 217 AAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGR 276
           AAYGAYVRPMP+SNE+GLRMLIGG  REASVLG+Y+TPLFSYYSYHGPVFRVMLR++RG+
Sbjct: 204 AAYGAYVRPMPYSNEVGLRMLIGGAVREASVLGYYITPLFSYYSYHGPVFRVMLRVNRGK 263

Query: 277 INKNRHYGFVSYCKKCGNSQGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYI 336
           + +NRHYGF++YC  CGNSQ FSW+E+GQ+SC C+ +  S SLV+SGPLWTGPLHSA YI
Sbjct: 264 LLENRHYGFITYCNNCGNSQEFSWAELGQISCSCSGSHGSRSLVISGPLWTGPLHSAAYI 323

Query: 337 EDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTM 396
             M+ LA +W WIGNG   DLEKLL QM+DESD +L  GYIKMDEVASRAK+NSPPL TM
Sbjct: 324 SKMINLAEEWGWIGNGAETDLEKLLKQMLDESDPRLKSGYIKMDEVASRAKINSPPLRTM 383

Query: 397 MSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALELQ 432
           MS M K GYA SRSHIASNAIKT+CPM+ CIRI+ ELQ
Sbjct: 384 MSAMQKEGYATSRSHIASNAIKTDCPMSVCIRISKELQ 421

BLAST of CmaCh04G010690 vs. TrEMBL
Match: A5ACL3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g02690 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.2e-166
Identity = 298/436 (68.35%), Postives = 350/436 (80.28%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLN--NCLPNN--RTERGI 60
           M+ +   TLS SPFL            S+ H         KPL+    LP +  RTERG+
Sbjct: 1   MILLSAKTLSSSPFLLR----------SSPHP---QNPNPKPLSASQALPKSEYRTERGL 60

Query: 61  EFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVL 120
            FD G++FFRHESATGRDLGVLAA+LY+KSKGS+RVLDA+CGCGIRSLRYLVEAEADFVL
Sbjct: 61  TFDSGNSFFRHESATGRDLGVLAASLYKKSKGSVRVLDAMCGCGIRSLRYLVEAEADFVL 120

Query: 121 ANDANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGS 180
           ANDAN+     I++NLS+   G GE RRW+VT+ DANRVM+E YL++DF+D IDVDSFGS
Sbjct: 121 ANDANENYGRTILENLSQGLGGSGEERRWVVTHTDANRVMTECYLRRDFYDLIDVDSFGS 180

Query: 181 DSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIG 240
           +S+FLR A NALKLDGL YVTSTDG++SGGH P  SLA+YGA+VRPMP+SNE+GLRMLIG
Sbjct: 181 ESSFLRSAYNALKLDGLFYVTSTDGYTSGGHRPHHSLASYGAFVRPMPYSNEVGLRMLIG 240

Query: 241 GVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFS 300
           G  REASVLG+ +TPLFSYYSYHGPVFRVMLR++RG++ +NR+YGF+SYC KCGNSQ FS
Sbjct: 241 GALREASVLGYRITPLFSYYSYHGPVFRVMLRMNRGKLPENRNYGFISYCTKCGNSQAFS 300

Query: 301 WSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEK 360
           W+E+G++SCPC+D +VS SLVVSGPLW GPLH+A Y+ +ML LA QW W GN     L K
Sbjct: 301 WNELGRISCPCSDAKVSSSLVVSGPLWIGPLHNASYLTEMLNLAEQWGWTGNDTETHLGK 360

Query: 361 LLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKT 420
           LL QM+DESD KLP GYIK+DEVASRAK+NSPPL TMMS + K GYA SRSHIASNAIKT
Sbjct: 361 LLKQMIDESDPKLPFGYIKLDEVASRAKINSPPLRTMMSALNKEGYAVSRSHIASNAIKT 420

Query: 421 NCPMAECIRIALELQQ 433
           NCPMA CIRIA ELQQ
Sbjct: 421 NCPMAVCIRIAKELQQ 423

BLAST of CmaCh04G010690 vs. TrEMBL
Match: A0A067JFV0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_03334 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 7.1e-164
Identity = 285/431 (66.13%), Postives = 344/431 (79.81%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           ML ++  TLS SPFL         F      + +F         NC    +TERGI F+ 
Sbjct: 1   MLILIPKTLSSSPFLLQNPLNVRNFEVRNPKVNKF---------NCKFELQTERGIVFET 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           G TFF+HESA GRDLGVLAA+LY++SKG+LRVLDA+CGCGIRS RYL+EA+ADFVLANDA
Sbjct: 61  GGTFFQHESAKGRDLGVLAASLYKQSKGNLRVLDAMCGCGIRSCRYLIEAKADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDE RG+I+ NL +V  GFG+ RRW V++ DANRV++E YLQ+DFFD ID+DSFGSDS F
Sbjct: 121 NDEYRGIILGNLKKVERGFGDDRRWAVSHFDANRVLTECYLQRDFFDLIDIDSFGSDSLF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LR A++ALKLDGLLY+TSTDG+SSGGH P  SLAAYGAY+RPMP  NEIGLRMLIGG  R
Sbjct: 181 LRSAMSALKLDGLLYLTSTDGYSSGGHRPHCSLAAYGAYIRPMPSCNEIGLRMLIGGAVR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EAS+LG++VTPLFSYYSYHGPVFRVML++++G+ ++NRHYGF+SYC +CGN+Q FSW E+
Sbjct: 241 EASLLGYHVTPLFSYYSYHGPVFRVMLQVNKGKDHENRHYGFISYCNQCGNTQSFSWEEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQ SC CN +  S  + VSGPLWTGPLH+A +I +ML LA QW WIGN  G DL+KLL +
Sbjct: 301 GQSSCSCNSSNDSSPVTVSGPLWTGPLHNATFITEMLNLAEQWGWIGNDVGADLDKLLKR 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESD +LP GYIKMDE+ASR K+NSP L TMMS ++K GYAA+RSHIASNAIKT+CPM
Sbjct: 361 MLDESDPRLPFGYIKMDEMASRGKINSPSLKTMMSTLVKKGYAATRSHIASNAIKTDCPM 420

Query: 421 AECIRIALELQ 432
           + CIRIA ELQ
Sbjct: 421 SMCIRIAKELQ 422

BLAST of CmaCh04G010690 vs. TAIR10
Match: AT3G56330.1 (AT3G56330.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 543.9 bits (1400), Expect = 9.2e-155
Identity = 266/432 (61.57%), Postives = 332/432 (76.85%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTIS-HCFSSSTIHLFQFSQSQKKPLNNCLPNNRT--ERGIE 60
           +LT+   TLS S F    ++ +  C S  +       +S K   +  L  +    ER +E
Sbjct: 2   LLTLSPKTLSSSSFFTVHKSQNPKCKSPDSCRF----KSLKCSFDRILVKSEVQIERNLE 61

Query: 61  FDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLA 120
           F+ G+TFFRHESA GRDLGVL+A LY++S GSLRVLDA+CGCGIRSLRYLVEAEADFV+A
Sbjct: 62  FETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMA 121

Query: 121 NDANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSD 180
           NDAND+NR VI  NLS+V  G G+ RRW+VT+  AN+ M E Y+  DFFD ID+DSFGSD
Sbjct: 122 NDANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSD 181

Query: 181 STFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGG 240
           S+FLR A NAL+L GLLY+TSTDG+SSGGH P  SLAAYGA++RPMPF NEIGLRMLIGG
Sbjct: 182 SSFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGG 241

Query: 241 VAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSW 300
             REA++LG++VTPLFSYYSYHGPVFRVMLR+ RG+++++R+YGFV++C  CG+S    +
Sbjct: 242 AVREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRF 301

Query: 301 SEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKL 360
            E+G M CPC+DT+ S SLVVSGP+W GPLH A Y+ +ML LA +W W+  G G DL+KL
Sbjct: 302 DELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKL 361

Query: 361 LGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTN 420
           L  M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT+
Sbjct: 362 LSIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTD 421

Query: 421 CPMAECIRIALE 430
           CPM+  +RIA E
Sbjct: 422 CPMSHFVRIAKE 429

BLAST of CmaCh04G010690 vs. TAIR10
Match: AT5G15810.1 (AT5G15810.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 92.4 bits (228), Expect = 7.3e-19
Identity = 96/382 (25.13%), Postives = 156/382 (40.84%), Query Frame = 1

Query: 59  DIGDTFFRHESATGRDLGVLAAALYRKSKGSL---RVLDALCGCGIRSLRYLVEAEA-DF 118
           D G T   HE  T       A   Y  ++  L   RVL+AL   G+R+LRY  E E    
Sbjct: 186 DNGKTNGEHEVTTQDGPKEAAKTAYESARRELKPPRVLEALSASGLRALRYAREVEGIGQ 245

Query: 119 VLANDANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSF 178
           V+A D +  +     +N+     G   T +      DA   M      KD FD +D+D +
Sbjct: 246 VVALDNDPASVEACQRNIK--FNGLMSTSKVESHLTDARVHMLSH--PKD-FDVVDLDPY 305

Query: 179 GSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRML 238
           G+ S FL  A+ ++   GLL  T+TD     G +     + YG+Y     + +E+ LR+L
Sbjct: 306 GAPSIFLDSAVQSVADGGLLMCTATDMAVLCGANGEVCYSKYGSYPLKGKYCHEMALRIL 365

Query: 239 IGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQG 298
           +  +   A+    Y+ P+ S         RV +R+             +SY  +C     
Sbjct: 366 LASIESHANRYKRYIVPVLSVQMDF--YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDS 425

Query: 299 FSWSEVGQMSCPCND----------------TRVSESLVVSGPLWTGPLHSADYIEDMLT 358
           F    VG+ S P N+                T   +   + GP+W+ P+H  +++  +L 
Sbjct: 426 FHLQSVGR-SLPKNNSVRYLPGVGPVVPQDCTHCGKKYNMGGPIWSAPIHDQEWVNSILN 485

Query: 359 LAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMI 418
                +        D    +   + E    +P+ ++ +  +++  K  SP      S +I
Sbjct: 486 GVKSMK--DRYPAYDRICAVLTTISEELPDVPL-FLSLHSLSATLKCTSPSAALFRSAVI 545

Query: 419 KAGYAASRSHIASNAIKTNCPM 421
            A Y  S SH+    IKT+ PM
Sbjct: 546 NAKYRVSGSHVNPLGIKTDAPM 556

BLAST of CmaCh04G010690 vs. TAIR10
Match: AT3G02320.1 (AT3G02320.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 83.2 bits (204), Expect = 4.4e-16
Identity = 83/356 (23.31%), Postives = 147/356 (41.29%), Query Frame = 1

Query: 91  RVLDALCGCGIRSLRYLVEAEA-DFVLANDANDENRGVIVKNL--------SRVAEGFGE 150
           +VL+AL   G+R+LRY  E E    V+A D +  +     +N+        S+V     +
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHTD 182

Query: 151 TRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDG 210
            R  M+T+                FD +D+D +GS S FL  AI ++   GLL  T+TD 
Sbjct: 183 ARVHMLTHPKE-------------FDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDM 242

Query: 211 FSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGP 270
               G +     + YG+Y     + +E+ LR+L+  +   A+    Y+ P+ S       
Sbjct: 243 AVLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF-- 302

Query: 271 VFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEVGQMSCPCNDT----------- 330
             RV +R+             +SY  +C     F    VG+ S P N++           
Sbjct: 303 YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVV 362

Query: 331 -----RVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 390
                   +   + GP+W+ P+H  +++  +L      +     +    +++   +   S
Sbjct: 363 KQDCNHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK----DRYPAYDRISAVLTTVS 422

Query: 391 DSKLPVG-YIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
           +  L V  ++ +  + +  K  SP      S +I A Y  S +H+    +KT+ PM
Sbjct: 423 EELLDVPLFLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

BLAST of CmaCh04G010690 vs. NCBI nr
Match: gi|778701515|ref|XP_011655038.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus])

HSP 1 Score: 799.7 bits (2064), Expect = 2.6e-228
Identity = 395/441 (89.57%), Postives = 415/441 (94.10%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTV   TLSPSPFL   QT SH  +SS+IHLFQFS S K PLNNCL NNRTERG++FDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           AECIRIA +LQ++CLG HQVT
Sbjct: 421 AECIRIA-QLQRNCLGAHQVT 440

BLAST of CmaCh04G010690 vs. NCBI nr
Match: gi|659114163|ref|XP_008456934.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 796.6 bits (2056), Expect = 2.2e-227
Identity = 391/441 (88.66%), Postives = 416/441 (94.33%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           A+CIRIA +LQQ+CLGVHQVT
Sbjct: 421 ADCIRIA-QLQQNCLGVHQVT 440

BLAST of CmaCh04G010690 vs. NCBI nr
Match: gi|659114167|ref|XP_008456937.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 723.8 bits (1867), Expect = 1.8e-205
Identity = 353/399 (88.47%), Postives = 375/399 (93.98%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIK 400
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIK
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

BLAST of CmaCh04G010690 vs. NCBI nr
Match: gi|595999826|ref|XP_007218021.1| (hypothetical protein PRUPE_ppa005915mg [Prunus persica])

HSP 1 Score: 604.7 bits (1558), Expect = 1.2e-169
Identity = 300/434 (69.12%), Postives = 354/434 (81.57%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPL--NNCLPNNRTERGIEF 60
           M+T+   TLSP  FL +        +S    + Q+S S  KP   +NC    +TERG++F
Sbjct: 1   MITLTPKTLSPFSFLCNPIPKHQNQNSKYTIVTQYSDSNSKPTFNSNCESEFQTERGLKF 60

Query: 61  DIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLAN 120
           DIG+TFFRHESATGRDLGVLAAALY++SKG LRVLDALCGCGIRSLRYL+EAEADFVLAN
Sbjct: 61  DIGETFFRHESATGRDLGVLAAALYKRSKGKLRVLDALCGCGIRSLRYLIEAEADFVLAN 120

Query: 121 DANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDS 180
           DAND+NR VI +NLS+V  G  +  RW VT  DANR+M+E YL++DFFD ID+DSFGSDS
Sbjct: 121 DANDDNRKVITENLSQVESG--DDPRWAVTLSDANRIMTECYLKRDFFDLIDIDSFGSDS 180

Query: 181 TFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGV 240
           +FLR AINALKLDGLLYVTSTDGFSSGG  P Q+LAAYGAYVRPMP+SNE+GLRMLIGG 
Sbjct: 181 SFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRMLIGGA 240

Query: 241 AREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWS 300
            R ASVLG+ VTPLFSYYSYHGP+FRVMLR++RG++  NR+YGF+SYC  CG+SQ +SW+
Sbjct: 241 VRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQEYSWA 300

Query: 301 EVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLL 360
           E+G+MSCPC+D++VS+SL+VSGPLWTGPLH   YI +ML LA +W W G+G G DL+KLL
Sbjct: 301 ELGRMSCPCSDSKVSKSLIVSGPLWTGPLHDTIYITEMLHLAEEWGWAGSGSGTDLDKLL 360

Query: 361 GQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNC 420
            QMV ESD +LP GY K+DEVASRAKVNSPPL  +MS + K GYAASRSHI SNAIKTNC
Sbjct: 361 NQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAIKTNC 420

Query: 421 PMAECIRIALELQQ 433
            MA CIRIA +LQQ
Sbjct: 421 SMAGCIRIAKQLQQ 432

BLAST of CmaCh04G010690 vs. NCBI nr
Match: gi|645256266|ref|XP_008233872.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Prunus mume])

HSP 1 Score: 601.3 bits (1549), Expect = 1.4e-168
Identity = 298/434 (68.66%), Postives = 351/434 (80.88%), Query Frame = 1

Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPL--NNCLPNNRTERGIEF 60
           M+T+   TLSP  FL +        +S    + Q+S S  KP   +NC    +TERG++F
Sbjct: 1   MITLTPKTLSPFSFLCNPIPKHQNQNSKYTIVTQYSDSSSKPTFNSNCESEFQTERGLKF 60

Query: 61  DIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLAN 120
           DIG+TFFRHESATGRDLGVLAAALY++SK  LRVLDALCGCGIRSLRYL+EAEADFVLAN
Sbjct: 61  DIGETFFRHESATGRDLGVLAAALYKRSKDKLRVLDALCGCGIRSLRYLIEAEADFVLAN 120

Query: 121 DANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDS 180
           DAND+N  VI +NLS+V    G+  RW VT  DANR+M+E YL++DFFD ID+DSFGSDS
Sbjct: 121 DANDDNGKVITENLSQVESSSGDDPRWAVTLLDANRIMTECYLKRDFFDLIDIDSFGSDS 180

Query: 181 TFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGV 240
           +FLR AINALKLDGLLYVTSTDGFSSGG  P Q+LAAYGAYVRPMP+SNE+GLRMLIGG 
Sbjct: 181 SFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRMLIGGA 240

Query: 241 AREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWS 300
            R ASVLG+ VTPLFSYYSYHGP+FRVMLR++RG++  NR+YGF+SYC  CG+SQ +SW+
Sbjct: 241 VRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQEYSWA 300

Query: 301 EVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLL 360
           E+G+MSCPC+D++VS+SLVVSGPLWTGPLH   YI +ML LA +W W G+G   DL+KLL
Sbjct: 301 ELGRMSCPCSDSKVSKSLVVSGPLWTGPLHDTVYITEMLHLAEEWGWAGSGSRTDLDKLL 360

Query: 361 GQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNC 420
            QMV ESD +LP GY K+DEVASRAKVNSPPL  +MS + K GYAASRSHI SNAIKTNC
Sbjct: 361 NQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAIKTNC 420

Query: 421 PMAECIRIALELQQ 433
            MA CIRIA +LQQ
Sbjct: 421 SMAGCIRIAKQLQQ 434