Carg00173 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg00173
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Location	Cucurbita_argyrosperma_scaffold_001 : 891602 .. 894176 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGCGCCACCGGACGCGACTTGGGCGTACTCGCGGCGGCGCTTTACCGTAAATCCAAGGGAAGTCTTCGGGTTCTCGACGCATTGTGCGGTTGCGGGATTCGGTCGCTTCGGTACTTGGTCGAGGCTGAGGCTGATTTTGTGTTGGCGAATGACGCCAATGACGAAAATCGGGGGGTTATAGTAAAGAATCTATCGAGGGTTGCGGAAGGTTTTGGGGAGATTCGGCGGTGGATGGTTACCAATTGTGATGCGAACAGGGTTATGTCGGAGTTCTATTTGCAAAGGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTTTTGAGGCCCGCTATTAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGCGGTCATCACCCTTCTCAGTGAGTGCACAATCTTATCTGTTACAATTTGTATTCCATTACTTGTTTGCTTTAACCGCTTAATTGAAAATCTTGATATGAAATGTGACCGCTAAAATCTTGATCAATGTAAGGGCTTTTATCTATATATAAATGATTGAATATATAACATTATTTTAGTTTGAAGTTTTAGTAGTTTCATATGGTATTAGCTCACTCTTATTGAGTCTTAAAGCCGACGTATTGGTTTAAGAAAATTTCAAAACTGAAGATAGAGCCGAATTTTCAAAAAGCTTTACTGACAAAACCAGCAAAAATTGGAAAGGCATACCAAGACTAAGAGAATTGACCCTAACCATTCCGAATGTTGTTTTTTTTGGTTTTGACTTGTACCCTTGTGCTAAGTTTTAAGTCTGCAAGCGAAATGAGTGCTAAGTAGTAGTTTGAATATTGGAGGGTAATAATATGAAGGTGTATGCTATACTATGGCTGGTGTGGTAATTAAAAATTCCTTTTTCCTTTCTCTTGTTTCTTTCTTTTTTTTTTTTTTTTTTTTTCTCTCTCTCTCTCTTTCTAGATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGTAACGCCACTCTTTTCGTATTATTCATACCATGGACCTGTTTTTAGAGTTATGCTTCGAATCAATCGAGGGAGGATCAATAAAAACAGGTGATAGTTGACGAGTTTAGAATGTCTGCTAGTGCTGGATGTATCTTGTATGATAATTGATGATGCTTGATTCATGATCCCACTATTTATGTCAGGCATTATGGTTTCGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGCTTTTTCCTGGAGTGAGGTTGGACAGATGAGTTGCCCTTGCAATGATACAAGGGTAAGACAATGATTATGATTGACATTCTGCTACTCTTTTGAGCTTAAGCATCCACTGGTTTCAGCAGCTTCCATATTTTAAACTGATACCGAAAGTTCCTCATATAATTTGCATGGTCCAGATAGCCATGGCTTCAAGTTGAATATGCTTGAATTTGTTGTTTGCTTATTCACTGGGTGCTTTCGTTCTAATGTAAATACTTGCAACATTTAATCTTGTAGTTGGAGATACATTAAAAAAAAATTTCATGTTAGGTTTCCGAGTCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAATGCAGACTACATTGAAGACATGTTGACCTTGGCTATGCAGTGGGAATGGATAGGCAATGGCCAAGGGAAGGATCTTGAGAAACTTCTGGGGCAAATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGTTTATTTTCTCATGGTTTAATATTTCATTTCTGTTTCTGGACCGACAATGTAAGACGTGATCTATCAACCCCCCATGCTTAGGTGGCAAGTCGAGCAAAAGTAAATTCGCCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGTGAGAATATAGGTAGTTAAGATTTGATAAATTGTGTGATGCATGTTATTGGCCACACGGCTAAACTGTTTTTGTTGTCATGAGATTCTAGTACATGCTGGAAATCAAAAAAATGTTGGCATAGACATGACGGGTTTTCTATGAGTATAGGACATAAATGTGGCCTGCATACGTTTATTTAAATTTATATAGTTTACAACTATATCATTTTCATACAAGAGAATTTAAAGTCTGTATGTCTAACTCATGTGTACTTGTCTACTGTACTTACAAGTACATCACGTGTTCAACATGTGTAGAAATGTCCGATACACGTTGAGTGTGGATAGTTTCCCGAACTAGAGTGTTTGCGTTTCATAGCTAGAGAACAGGATTTTGCGTGGAATGTAGATGCGTGAAGAGAAAAATAGTTCTCTCTTTCCTTTTTTCTCTCTTTCTGAGGCTTTTTGTTGTTGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAATGCAATCAAGACGAACTGTCCTATGGCGGAGTGCATCAGAATTGCTCTTGAACTGCAGCAGAGTTGTCTTGGTGTGCATCAAGCCACATGA

mRNA sequence

ATGAAAGCGCCACCGGACGCGACTTGGGCGTACTCGCGGCGGCGCTTTACCGTAAATCCAAGGGAAGTCTTCGGGTTCTCGACGCATTGTGCGGTTGCGGGATTCGGTCGCTTCGTAAAGAATCTATCGAGGGTTGCGGAAGGTTTTGGGGAGATTCGGCGGTGGATGGTTACCAATTGTGATGCGAACAGGGTTATGTCGGAGTTCTATTTGCAAAGGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTTTTGAGGCCCGCTATTAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGCGGTCATCACCCTTCTCAATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGTAACGCCACTCTTTTCGTATTATTCATACCATGGACCTGTTTTTAGAGTTATGCTTCGAATCAATCGAGGGAGGATCAATAAAAACAGGCATTATGGTTTCGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGCTTTTTCCTGGAGTGAGGTTGGACAGATGAGTTGCCCTTGCAATGATACAAGGGTTTCCGAGTCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAATGCAGACTACATTGAAGACATGTTGACCTTGGCTATGCAGTGGGAATGGATAGGCAATGGCCAAGGGAAGGATCTTGAGAAACTTCTGGGGCAAATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGAGCAAAAGTAAATTCGCCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAATGCAATCAAGACGAACTGTCCTATGGCGGAGTGCATCAGAATTGCTCTTGAACTGCAGCAGAGTTGTCTTGGTGTGCATCAAGCCACATGA

Coding sequence (CDS)

ATGAAAGCGCCACCGGACGCGACTTGGGCGTACTCGCGGCGGCGCTTTACCGTAAATCCAAGGGAAGTCTTCGGGTTCTCGACGCATTGTGCGGTTGCGGGATTCGGTCGCTTCGTAAAGAATCTATCGAGGGTTGCGGAAGGTTTTGGGGAGATTCGGCGGTGGATGGTTACCAATTGTGATGCGAACAGGGTTATGTCGGAGTTCTATTTGCAAAGGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTTTTGAGGCCCGCTATTAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGCGGTCATCACCCTTCTCAATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGTAACGCCACTCTTTTCGTATTATTCATACCATGGACCTGTTTTTAGAGTTATGCTTCGAATCAATCGAGGGAGGATCAATAAAAACAGGCATTATGGTTTCGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGCTTTTTCCTGGAGTGAGGTTGGACAGATGAGTTGCCCTTGCAATGATACAAGGGTTTCCGAGTCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAATGCAGACTACATTGAAGACATGTTGACCTTGGCTATGCAGTGGGAATGGATAGGCAATGGCCAAGGGAAGGATCTTGAGAAACTTCTGGGGCAAATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGAGCAAAAGTAAATTCGCCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAATGCAATCAAGACGAACTGTCCTATGGCGGAGTGCATCAGAATTGCTCTTGAACTGCAGCAGAGTTGTCTTGGTGTGCATCAAGCCACATGA

Protein sequence

MKAPPDATWAYSRRRFTVNPREVFGFSTHCAVAGFGRFVKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALELQQSCLGVHQAT

Homology

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BLAST of Carg00173 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 639.8 bits (1649), Expect = 5.4e-180
Identity = 311/316 (98.42%), Postives = 313/316 (99.05%), Query Frame = 0


Query: 36  GRFVKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAI 95
           G  VKNLSRVAEGFGE RRWMVTNCDANRVMSEFYLQ+DFFDFIDVDSFGSDSTFLRPAI
Sbjct: 126 GVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLRPAI 185

Query: 96  NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL 155
           NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL
Sbjct: 186 NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL 245

Query: 156 GFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSC 215
           GFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSC
Sbjct: 246 GFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSC 305

Query: 216 PCNDTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 275
           PCNDTRVSESLVVSGPLWTGPLH+ADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES
Sbjct: 306 PCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 365

Query: 276 DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIR 335
           DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIR
Sbjct: 366 DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIR 425

Query: 336 IALELQQSCLGVHQAT 352
           IALELQQSCLGVHQAT
Sbjct: 426 IALELQQSCLGVHQAT 441

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BLAST of Carg00173 vs. NCBI nr
Match: XP_022986521.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima])

HSP 1 Score: 634.8 bits (1636), Expect = 1.7e-178
Identity = 308/316 (97.47%), Postives = 311/316 (98.42%), Query Frame = 0


Query: 36  GRFVKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAI 95
           G  VKNLSRVAEGFGE RRWMVTNCDANRVMSEFYLQ+DFFDFIDVDSFGSDSTFLRPAI
Sbjct: 126 GVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLRPAI 185

Query: 96  NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL 155
           NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL
Sbjct: 186 NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL 245

Query: 156 GFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSC 215
           GFYVTPLFSYYSYHGPVFRVMLRI+RGRINKNRHYGFVSYCKKCGNSQ FSWSEVGQMSC
Sbjct: 246 GFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEVGQMSC 305

Query: 216 PCNDTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 275
           PCNDTRVSESLVVSGPLWTGPLH+ADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES
Sbjct: 306 PCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 365

Query: 276 DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIR 335
           DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIR
Sbjct: 366 DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIR 425

Query: 336 IALELQQSCLGVHQAT 352
           IALELQQSCLGVHQ T
Sbjct: 426 IALELQQSCLGVHQVT 441

[Close]

BLAST of Carg00173 vs. NCBI nr
Match: XP_023529370.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 633.6 bits (1633), Expect = 3.9e-178
Identity = 307/316 (97.15%), Postives = 311/316 (98.42%), Query Frame = 0


Query: 36  GRFVKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAI 95
           G  VKNLS+VAEGFGE RRWMVTNCDANRVMSEFYLQ+DFFDFIDVDSFGSDSTFLRPAI
Sbjct: 126 GVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLRPAI 185

Query: 96  NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL 155
           NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL
Sbjct: 186 NALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVL 245

Query: 156 GFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSC 215
           GFYVTPLFSYYSYHGPVFRVMLRI+RGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSC
Sbjct: 246 GFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSC 305

Query: 216 PCNDTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 275
           PCNDTRVSESLVVSGPLWTGPLH+ADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES
Sbjct: 306 PCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 365

Query: 276 DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIR 335
           DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM ECIR
Sbjct: 366 DSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMTECIR 425

Query: 336 IALELQQSCLGVHQAT 352
           IALELQQSCLGVHQ T
Sbjct: 426 IALELQQSCLGVHQVT 441

[Close]

BLAST of Carg00173 vs. NCBI nr
Match: XP_011655038.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_5G220920 [Cucumis sativus])

HSP 1 Score: 592.0 bits (1525), Expect = 1.3e-165
Identity = 286/313 (91.37%), Postives = 302/313 (96.49%), Query Frame = 0


Query: 39  VKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINAL 98
           VKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL++DFFDFIDVDSFGSDS+FLRPAINAL
Sbjct: 129 VKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINAL 188

Query: 99  KLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY 158
           KLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY
Sbjct: 189 KLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY 248

Query: 159 VTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCN 218
            TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+GQMSCPCN
Sbjct: 249 ATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCN 308

Query: 219 DTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSK 278
           D+RVSESLVVSGPLW GPLH+ADYIED+LTLA QWEWIGNGQGKDLEKLL QMVDESDSK
Sbjct: 309 DSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSK 368

Query: 279 LPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIAL 338
           LPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIA 
Sbjct: 369 LPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIA- 428

Query: 339 ELQQSCLGVHQAT 352
           +LQ++CLG HQ T
Sbjct: 429 QLQRNCLGAHQVT 440

[Close]

BLAST of Carg00173 vs. NCBI nr
Match: XP_008456934.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >XP_008456936.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 592.0 bits (1525), Expect = 1.3e-165
Identity = 284/313 (90.73%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 39  VKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINAL 98
           VKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL++DFFDFIDVDSFGSDS+FLRPAINAL
Sbjct: 129 VKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINAL 188

Query: 99  KLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY 158
           KLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAREASVLG+Y
Sbjct: 189 KLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYY 248

Query: 159 VTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCN 218
            TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+GQMSCPCN
Sbjct: 249 ATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCN 308

Query: 219 DTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSK 278
           D+RVSESLVVSGPLW GPLH+ADYIED+LTLA QWEWIGNGQGKDLEKLL QM+DESDSK
Sbjct: 309 DSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDESDSK 368

Query: 279 LPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIAL 338
           LPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPMA+CIRIA 
Sbjct: 369 LPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMADCIRIA- 428

Query: 339 ELQQSCLGVHQAT 352
           +LQQ+CLGVHQ T
Sbjct: 429 QLQQNCLGVHQVT 440

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BLAST of Carg00173 vs. TAIR10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 422.2 bits (1084), Expect = 3.2e-118
Identity = 193/299 (64.55%), Postives = 243/299 (81.27%), Query Frame = 0


Query: 41  NLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINALKL 100
           NLS+V  G G+ RRW+VT+  AN+ M E Y+  DFFD ID+DSFGSDS+FLR A NAL+L
Sbjct: 131 NLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRL 190

Query: 101 DGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVT 160
            GLLY+TSTDG+SSGGH P  SLAAYGA++RPMPF NEIGLRMLIGG  REA++LG++VT
Sbjct: 191 GGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVT 250

Query: 161 PLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDT 220
           PLFSYYSYHGPVFRVMLR++RG+++++R+YGFV++C  CG+S    + E+G M CPC+DT
Sbjct: 251 PLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFDELGLMGCPCSDT 310

Query: 221 RVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLP 280
           + S SLVVSGP+W GPLH+A Y+ +ML LA +W W+  G G DL+KLL  M++ESD +LP
Sbjct: 311 KASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSIMIEESDPRLP 370

Query: 281 VGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALE 340
            GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT+CPM+  +RIA E
Sbjct: 371 PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPMSHFVRIAKE 429

[Close]

BLAST of Carg00173 vs. TAIR10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 77.8 bits (190), Expect = 1.5e-14
Identity = 63/272 (23.16%), Postives = 116/272 (42.65%), Query Frame = 0


Query: 76  FDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPF 135
           FD +D+D +GS S FL  AI ++   GLL  T+TD     G +     + YG+Y     +
Sbjct: 194 FDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVLCGGNGEVCYSKYGSYPLRAKY 253

Query: 136 SNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSY 195
            +E+ LR+L+  +   A+    Y+ P+ S         RV +R+             +SY
Sbjct: 254 CHEMALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVRVYTSASAMKNTPLKLSY 313

Query: 196 CKKCGNSQAFSWSEVGQMSCPCNDT----------------RVSESLVVSGPLWTGPLHN 255
             +C    +F    VG+ S P N++                   +   + GP+W+ P+H+
Sbjct: 314 VYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVVKQDCNHCGKKYNMGGPIWSAPMHD 373

Query: 256 ADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVG-YIKMDEVASRAKVNSP 315
            +++  +L      +     +    +++   +   S+  L V  ++ +  + +  K  SP
Sbjct: 374 PEWVTSILNSVKSMK----DRYPAYDRISAVLTTVSEELLDVPLFLSLHNLCATLKCISP 433

Query: 316 PLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 331
                 S +I A Y  S +H+    +KT+ PM
Sbjct: 434 SAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

[Close]

BLAST of Carg00173 vs. Swiss-Prot
Match: sp|P81554|TRM1_PYRFU (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-25
Identity = 86/277 (31.05%), Postives = 134/277 (48.38%), Query Frame = 0


Query: 56  MVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSG 115
           ++ N DANR+M+E   +  +F FID+D FGS   FL  A+ ++K  G+L VT+TDG    
Sbjct: 117 VINNDDANRLMAE---KHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGVTATDGAPLC 176

Query: 116 GHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRV 175
           G HP   L  Y A        +E+G R+L+G +AR A+     +  L +YY  H   FR 
Sbjct: 177 GAHPKACLRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGMEVLLAYYKDH--YFRA 236

Query: 176 MLRINRGRINKN---RHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPL 235
            +++  G    +    + G+V + +K G  +     E   +S   N           GPL
Sbjct: 237 FVKLKDGAKKGDETLENLGYVYFDEKTGRFEV----EKSFLSTRPN---------AYGPL 296

Query: 236 WTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASR 295
           W GPL N   + +ML L +    + N   +++ KLL  + +E D  L   +     +  R
Sbjct: 297 WLGPLKNEKVVGEMLELLVSGFEVAN--YREVLKLLHMLHEELDIPL---FYDTHALGKR 356

Query: 296 AKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCP 330
            K+    L  ++ E+   GY A+R+H +   IKTN P
Sbjct: 357 LKIEPKKLGEIIKELKSMGYEATRTHFSPTGIKTNAP 370

[Close]

BLAST of Carg00173 vs. Swiss-Prot
Match: sp|B6YUU9|TRM1_THEON (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-25
Identity = 90/286 (31.47%), Postives = 141/286 (49.30%), Query Frame = 0


Query: 56  MVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSG 115
           +VTN DANR+M+E   Q  +FDF+D+D FGS   FL  A+ A+K  G+L +T+TD     
Sbjct: 115 VVTNADANRLMNE---QFRYFDFVDLDPFGSPMEFLDSALRAVKRRGVLAITATDTGVLC 174

Query: 116 GHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRV 175
           G + +  L  Y A        +E GLR+LIG + R A+     V  L +YY  H   FR 
Sbjct: 175 GAYRNACLRKYLAEPIRGELCHEAGLRILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRA 234

Query: 176 MLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTG 235
            L++  G    ++    + Y  +          E G+      +T +       GPLW G
Sbjct: 235 FLQLRNGAKTADKSMKQLGYLYQ---------DESGKFE--FENTFLPSKATAHGPLWLG 294

Query: 236 PLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKV 295
           PL N +++E++  L+ +   +G+   K  +K LG + DE +  +P  Y     VA R  +
Sbjct: 295 PLKNQEFVEELHRLSAEHP-LGS---KKTQKFLGLLKDELE--VPFFY-DTHAVARRHGL 354

Query: 296 NSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALELQ 342
               L  ++  + + GY A+R+H +  A+KT+ P  E +    ELQ
Sbjct: 355 EVRKLAGVIEILYEKGYKATRTHFSPTALKTDAPFEEVLEALRELQ 377

[Close]

BLAST of Carg00173 vs. Swiss-Prot
Match: sp|Q9V1P3|TRM1_PYRAB (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 8.9e-25
Identity = 80/277 (28.88%), Postives = 135/277 (48.74%), Query Frame = 0


Query: 56  MVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSG 115
           +V + DANR+M+E   +  +F FID+D FGS   FL  A+ ++K  G+L +T+TDG    
Sbjct: 115 VVNHDDANRLMAE---KHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLC 174

Query: 116 GHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRV 175
           G HP   +  Y A        +E+G R+L+G VAR A+     +  + +YY  H   FR 
Sbjct: 175 GAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDH--YFRA 234

Query: 176 MLRINRGRINKN---RHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPL 235
            +++  G    +    + G++ + +  G        EV +   P             GP+
Sbjct: 235 FIKLKDGARKGDESLENLGYIYFDESTGK------FEVERSFLPSKPN-------AYGPV 294

Query: 236 WTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASR 295
           W GPL + + +E+ML ++ Q   +   + K   KLL  + DE D  L   +     +  R
Sbjct: 295 WLGPLKSQEIVEEMLEISQQ---LSLARKKQAVKLLKILKDELDVPL---FYDTHGLGRR 354

Query: 296 AKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCP 330
            K+ +  +  +++E+   GY ASR+H +   +KT+ P
Sbjct: 355 LKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAP 367

[Close]

BLAST of Carg00173 vs. Swiss-Prot
Match: sp|Q9YDY7|TRM1_AERPE (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=trm1 PE=3 SV=3)

HSP 1 Score: 112.8 bits (281), Expect = 7.5e-24
Identity = 86/301 (28.57%), Postives = 149/301 (49.50%), Query Frame = 0


Query: 55  WMVTNCDANRVMSEFYLQRDF---FDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTD- 114
           +M+ N ++N +M  F+L R+       ID+D +GS + F+  A+       ++ +T+TD 
Sbjct: 122 YMIFNKESNSLM--FHLSRERPTPVSLIDIDPYGSPAPFVDAALALSGKGTVVAMTATDL 181

Query: 115 GFSSGGHHPSQSLAAYGAY----VRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYY 174
               GG    ++ AA   Y    V   P S E GLR+L+G VAR A+     V PL +YY
Sbjct: 182 AVLEGG----KARAAVRRYMLRSVSKTPVSKETGLRVLLGYVARVAAAHDKAVKPLLAYY 241

Query: 175 SYHGPVFRVMLRINRGRINKN----RHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRV 234
           + H   +RV + + RG    +     + G + YC + G + A S++E    +C       
Sbjct: 242 ADH--YYRVYVAVERGARRSDSMLEENLGRLVYCPETGVALALSYAEDPASACG------ 301

Query: 235 SESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVG 294
              +V + P W G L +  ++E ML +A++  W+G      +EKLL  +  E+       
Sbjct: 302 GSYVVAADPAWIGSLGDQAFLEAMLNIAVEAVWLGT--RPRVEKLLNTLHGEAPLSPRSI 361

Query: 295 YIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALELQQ 344
           Y+ +  VAS+A+VN+P  + ++  +   GY A  +H +   ++T+ P  E +   ++L  
Sbjct: 362 YVSLTSVASKARVNTPKKSKVVELLRSMGYRAVATHFSGEGVRTDAPWGEVLEAVVKLGS 406

[Close]

BLAST of Carg00173 vs. Swiss-Prot
Match: sp|Q9P804|TRM1_SCHPO (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=trm1 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 9.8e-24
Identity = 86/291 (29.55%), Postives = 137/291 (47.08%), Query Frame = 0


Query: 61  DANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTD-GFSSGGHHP 120
           DAN VM   ++ +  +D ID+D +GS + FL  A+ ++  DGLL +T TD    +G  +P
Sbjct: 183 DANAVM---HMNKFHYDVIDLDPYGSAAPFLDAAVQSVSKDGLLCITCTDSAVLAGNAYP 242

Query: 121 SQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFS----YYSYHGPVFRV 180
            +  + YG       F +E  +R L+  +A  A+  G  + PL S    +Y      FRV
Sbjct: 243 EKCFSNYGGSSLRSNFCHEQAVRHLLYAIAASAAKYGRAIKPLLSLSIDFY------FRV 302

Query: 181 MLRINRGRI---NKNRHYGFVSYCKKCGN--SQAFSWSEVGQMSCPCNDTRVSESLV--- 240
            ++I    +   N       + +C  CG+   Q    +  G++      +  S   V   
Sbjct: 303 FVQIKAKPVLVKNLQSQSLLIYHCSGCGSFAEQPLGKTSPGRLPGTTKFSNASGPPVSAN 362

Query: 241 ---------VSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSK 300
                    V GPLW GPLH+A++++ M  +A   +    G  + +  +L  + DE    
Sbjct: 363 CEHCGYVHHVGGPLWGGPLHDAEFLKKMRAIAEDLDPEVYGTKRRILGMLA-LADEELPD 422

Query: 301 LPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCP 330
           +P  Y  + ++ S  +  SPP N  +S ++ AGY  S SH  SNAIKTN P
Sbjct: 423 VPF-YFVLSQICSVLRSQSPPQNIFVSALLNAGYRVSGSHAKSNAIKTNAP 462

[Close]

BLAST of Carg00173 vs. TrEMBL
Match: tr|A0A1S3C509|A0A1S3C509_CUCME (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 8.5e-166
Identity = 284/313 (90.73%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 39  VKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINAL 98
           VKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL++DFFDFIDVDSFGSDS+FLRPAINAL
Sbjct: 129 VKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINAL 188

Query: 99  KLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY 158
           KLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAREASVLG+Y
Sbjct: 189 KLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYY 248

Query: 159 VTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCN 218
            TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+GQMSCPCN
Sbjct: 249 ATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCN 308

Query: 219 DTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSK 278
           D+RVSESLVVSGPLW GPLH+ADYIED+LTLA QWEWIGNGQGKDLEKLL QM+DESDSK
Sbjct: 309 DSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDESDSK 368

Query: 279 LPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIAL 338
           LPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPMA+CIRIA 
Sbjct: 369 LPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMADCIRIA- 428

Query: 339 ELQQSCLGVHQAT 352
           +LQQ+CLGVHQ T
Sbjct: 429 QLQQNCLGVHQVT 440

[Close]

BLAST of Carg00173 vs. TrEMBL
Match: tr|A0A0A0KP99|A0A0A0KP99_CUCSA (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 8.5e-166
Identity = 286/313 (91.37%), Postives = 302/313 (96.49%), Query Frame = 0


Query: 39  VKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINAL 98
           VKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL++DFFDFIDVDSFGSDS+FLRPAINAL
Sbjct: 129 VKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINAL 188

Query: 99  KLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY 158
           KLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY
Sbjct: 189 KLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY 248

Query: 159 VTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCN 218
            TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+GQMSCPCN
Sbjct: 249 ATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCN 308

Query: 219 DTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSK 278
           D+RVSESLVVSGPLW GPLH+ADYIED+LTLA QWEWIGNGQGKDLEKLL QMVDESDSK
Sbjct: 309 DSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSK 368

Query: 279 LPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIAL 338
           LPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIA 
Sbjct: 369 LPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIA- 428

Query: 339 ELQQSCLGVHQAT 352
           +LQ++CLG HQ T
Sbjct: 429 QLQRNCLGAHQVT 440

[Close]

BLAST of Carg00173 vs. TrEMBL
Match: tr|A0A1S3C4F5|A0A1S3C4F5_CUCME (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 2.4e-144
Identity = 247/271 (91.14%), Postives = 264/271 (97.42%), Query Frame = 0


Query: 39  VKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINAL 98
           VKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL++DFFDFIDVDSFGSDS+FLRPAINAL
Sbjct: 129 VKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINAL 188

Query: 99  KLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFY 158
           KLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAREASVLG+Y
Sbjct: 189 KLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYY 248

Query: 159 VTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCN 218
            TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+GQMSCPCN
Sbjct: 249 ATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCN 308

Query: 219 DTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSK 278
           D+RVSESLVVSGPLW GPLH+ADYIED+LTLA QWEWIGNGQGKDLEKLL QM+DESDSK
Sbjct: 309 DSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDESDSK 368

Query: 279 LPVGYIKMDEVASRAKVNSPPLNTMMSEMIK 310
           LPVGYIKMDEVASRAKVNSPPL+TMMSEMIK
Sbjct: 369 LPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

[Close]

BLAST of Carg00173 vs. TrEMBL
Match: tr|A0A2N9J6K0|A0A2N9J6K0_FAGSY (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60350 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 1.5e-130
Identity = 227/302 (75.17%), Postives = 262/302 (86.75%), Query Frame = 0


Query: 43  SRVAEGFG-EIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRPAINALKLD 102
           SRV  GFG + RRW VT+ DANR+M+E YLQRD FD IDVDSFGSDS+FLR A+NALKLD
Sbjct: 126 SRVPRGFGDDERRWAVTHFDANRLMTECYLQRDLFDLIDVDSFGSDSSFLRSAMNALKLD 185

Query: 103 GLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTP 162
           GLLY+TSTDG++SGGH PS SLAAYGAYVRPMP+SNE+GLRMLIGG  +EASVLG+ VTP
Sbjct: 186 GLLYITSTDGYTSGGHRPSHSLAAYGAYVRPMPYSNEVGLRMLIGGAVKEASVLGYSVTP 245

Query: 163 LFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTR 222
           LFSYYSYHGPVFRVMLR+NRG++  +R+YGF+SYC +CGNS +FSW ++GQ+SCPC+D++
Sbjct: 246 LFSYYSYHGPVFRVMLRLNRGKLVDDRYYGFISYCHQCGNSHSFSWDQLGQISCPCSDSK 305

Query: 223 VSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPV 282
           VS SLVVSGPLWTGPLH+A Y+ +ML LA Q  W+G+G+G  LEKLL QMVDESD  LP+
Sbjct: 306 VSSSLVVSGPLWTGPLHDAAYVTEMLNLAEQRGWVGSGKGTHLEKLLKQMVDESDPNLPI 365

Query: 283 GYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALELQ 342
           GYIKMDEVASRAK+NSPPL TMMS M K GYAASRSHIASNAIKTNCPM  CIRIA  LQ
Sbjct: 366 GYIKMDEVASRAKINSPPLMTMMSTMHKEGYAASRSHIASNAIKTNCPMTVCIRIAKNLQ 425

Query: 343 QS 344
           QS
Sbjct: 426 QS 427

[Close]

BLAST of Carg00173 vs. TrEMBL
Match: tr|A5ACL3|A5ACL3_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_13s0067g02690 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 2.6e-130
Identity = 226/309 (73.14%), Postives = 263/309 (85.11%), Query Frame = 0


Query: 35  FGR-FVKNLSRVAEGFGEIRRWMVTNCDANRVMSEFYLQRDFFDFIDVDSFGSDSTFLRP 94
           +GR  ++NLS+   G GE RRW+VT+ DANRVM+E YL+RDF+D IDVDSFGS+S+FLR 
Sbjct: 115 YGRTILENLSQGLGGSGEERRWVVTHTDANRVMTECYLRRDFYDLIDVDSFGSESSFLRS 174

Query: 95  AINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREAS 154
           A NALKLDGL YVTSTDG++SGGH P  SLA+YGA+VRPMP+SNE+GLRMLIGG  REAS
Sbjct: 175 AYNALKLDGLFYVTSTDGYTSGGHRPHHSLASYGAFVRPMPYSNEVGLRMLIGGALREAS 234

Query: 155 VLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQM 214
           VLG+ +TPLFSYYSYHGPVFRVMLR+NRG++ +NR+YGF+SYC KCGNSQAFSW+E+G++
Sbjct: 235 VLGYRITPLFSYYSYHGPVFRVMLRMNRGKLPENRNYGFISYCTKCGNSQAFSWNELGRI 294

Query: 215 SCPCNDTRVSESLVVSGPLWTGPLHNADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVD 274
           SCPC+D +VS SLVVSGPLW GPLHNA Y+ +ML LA QW W GN     L KLL QM+D
Sbjct: 295 SCPCSDAKVSSSLVVSGPLWIGPLHNASYLTEMLNLAEQWGWTGNDTETHLGKLLKQMID 354

Query: 275 ESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAEC 334
           ESD KLP GYIK+DEVASRAK+NSPPL TMMS + K GYA SRSHIASNAIKTNCPMA C
Sbjct: 355 ESDPKLPFGYIKLDEVASRAKINSPPLRTMMSALNKEGYAVSRSHIASNAIKTNCPMAVC 414

Query: 335 IRIALELQQ 343
           IRIA ELQQ
Sbjct: 415 IRIAKELQQ 423

The following BLAST results are available for this feature:

BLAST of Carg00173 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943113.1	5.4e-180	98.42	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata]	[more]
XP_022986521.1	1.7e-178	97.47	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima]	[more]
XP_023529370.1	3.9e-178	97.15	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo]	[more]
XP_011655038.1	1.3e-165	91.37	PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011...	[more]
XP_008456934.1	1.3e-165	90.73	PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo]...	[more]

BLAST of Carg00173 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G56330.1	3.2e-118	64.55	N2,N2-dimethylguanosine tRNA methyltransferase	[more]
AT3G02320.1	1.5e-14	23.16	N2,N2-dimethylguanosine tRNA methyltransferase	[more]

BLAST of Carg00173 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P81554\|TRM1_PYRFU	1.4e-25	31.05	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ...	[more]
sp\|B6YUU9\|TRM1_THEON	1.4e-25	31.47	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N...	[more]
sp\|Q9V1P3\|TRM1_PYRAB	8.9e-25	28.88	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O...	[more]
sp\|Q9YDY7\|TRM1_AERPE	7.5e-24	28.57	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Aeropyrum pernix (strain ATCC 700...	[more]
sp\|Q9P804\|TRM1_SCHPO	9.8e-24	29.55	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Schizosaccharomyces pombe (strain...	[more]

BLAST of Carg00173 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3C509\|A0A1S3C509_CUCME	8.5e-166	90.73	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
tr\|A0A0A0KP99\|A0A0A0KP99_CUCSA	8.5e-166	91.37	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
tr\|A0A1S3C4F5\|A0A1S3C4F5_CUCME	2.4e-144	91.14	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
tr\|A0A2N9J6K0\|A0A2N9J6K0_FAGSY	1.5e-130	75.17	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_L...	[more]
tr\|A5ACL3\|A5ACL3_VITVI	2.6e-130	73.14	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_13s0067g02690 PE=3 SV=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0008033	tRNA processing

Vocabulary: **Molecular Function**
Term	Definition
GO:0004809	tRNA (guanine-N2-)-methyltransferase activity
GO:0003723	RNA binding

Vocabulary: **INTERPRO**
Term	Definition
IPR029063	SAM-dependent_MTases
IPR002905	Trm1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009451	RNA modification
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00173-RA	Carg00173-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002905	tRNA methyltransferase, Trm1	PFAM	PF02005	TRM	coord: 56..336 e-value: 2.8E-51 score: 174.8
IPR002905	tRNA methyltransferase, Trm1	PANTHER	PTHR10631	N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE	coord: 38..342
IPR002905	tRNA methyltransferase, Trm1	PROSITE	PS51626	SAM_MT_TRM1	coord: 1..337 score: 73.938
None	No IPR available	GENE3D	G3DSA:3.40.50.150		coord: 37..277 e-value: 5.8E-54 score: 185.0
None	No IPR available	GENE3D	G3DSA:3.30.56.70		coord: 282..338 e-value: 8.7E-12 score: 46.6
None	No IPR available	PANTHER	PTHR10631:SF9	N2, N2-DIMETHYLGUANOSINE TRNA METHYLTRANSFERASES-LIKE PROTEIN	coord: 38..342
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	SSF53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 53..337

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg00173	Csa5G220920	Cucumber (Chinese Long) v2	carcuB0694
Carg00173	MELO3C020140	Melon (DHL92) v3.5.1	carmeB0656
Carg00173	CSPI05G11470	Wild cucumber (PI 183967)	carcpiB0706
Carg00173	CmaCh04G010690	Cucurbita maxima (Rimu)	carcmaB0948
Carg00173	CmoCh04G011360	Cucurbita moschata (Rifu)	carcmoB0923
Carg00173	CsaV3_5G010870	Cucumber (Chinese Long) v3	carcucB0708
Carg00173	Cla97C07G133390	Watermelon (97103) v2	carwmbB0700
Carg00173	Bhi07G000423	Wax gourd	carwgoB0785

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg00173	Cucumber (Gy14) v2	carcgybB0670
Carg00173	Cucumber (Gy14) v2	carcgybB0673
Carg00173	Cucumber (Gy14) v1	carcgyB0745
Carg00173	Cucurbita maxima (Rimu)	carcmaB0940
Carg00173	Cucurbita maxima (Rimu)	carcmaB0943
Carg00173	Cucurbita maxima (Rimu)	carcmaB0949
Carg00173	Cucurbita maxima (Rimu)	carcmaB0950
Carg00173	Cucurbita maxima (Rimu)	carcmaB0952
Carg00173	Cucurbita moschata (Rifu)	carcmoB0914
Carg00173	Cucurbita moschata (Rifu)	carcmoB0918
Carg00173	Cucurbita moschata (Rifu)	carcmoB0924
Carg00173	Cucurbita moschata (Rifu)	carcmoB0925
Carg00173	Cucurbita moschata (Rifu)	carcmoB0927
Carg00173	Cucurbita pepo (Zucchini)	carcpeB0934
Carg00173	Cucurbita pepo (Zucchini)	carcpeB0938
Carg00173	Cucurbita pepo (Zucchini)	carcpeB0939
Carg00173	Cucurbita pepo (Zucchini)	carcpeB0944
Carg00173	Cucurbita pepo (Zucchini)	carcpeB0952
Carg00173	Wild cucumber (PI 183967)	carcpiB0709
Carg00173	Cucumber (Chinese Long) v2	carcuB0697
Carg00173	Cucumber (Chinese Long) v3	carcucB0711
Carg00173	Bottle gourd (USVL1VR-Ls)	carlsiB603
Carg00173	Bottle gourd (USVL1VR-Ls)	carlsiB605
Carg00173	Melon (DHL92) v3.6.1	carmedB0671
Carg00173	Watermelon (Charleston Gray)	carwcgB0644
Carg00173	Watermelon (Charleston Gray)	carwcgB0651
Carg00173	Watermelon (97103) v1	carwmB0667
Carg00173	Watermelon (97103) v2	carwmbB0689
Carg00173	Wax gourd	carwgoB0781
Carg00173	Silver-seed gourd	carcarB025
Carg00173	Silver-seed gourd	carcarB197
Carg00173	Silver-seed gourd	carcarB305
Carg00173	Silver-seed gourd	carcarB570
Carg00173	Silver-seed gourd	carcarB573