BLAST of CSPI05G11470 vs. Swiss-Prot
Match:
TRM1_PYRAB (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=trm1 PE=3 SV=2)
HSP 1 Score: 139.0 bits (349), Expect = 1.3e-31
Identity = 105/369 (28.46%), Postives = 180/369 (48.78%), Query Frame = 1
Query: 64 FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 123
F+ A RD+ V+ L K R+ VLDAL GIR +R+ +E+ A+ + ND +++
Sbjct: 27 FYNPRMALNRDIAVV---LLNVLKPRI-VLDALSATGIRGIRFALETPAEEIWMNDISED 86
Query: 124 NRRVIVKNL-----SRVAENIG-----DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
++ KN+ + E+ G + +V + DANR+M E K +F FID+D
Sbjct: 87 AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146
Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 243
FGS FL A+ ++K G+L +T+TDG G P + Y A +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206
Query: 244 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN---RHYGFVSYCEKCG 303
L+G VAR A+ + +YY H FR +++ G + + G++ + E G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266
Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 363
E+ + P + GP+W+GPL S + +E++L +++Q + +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326
Query: 364 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 420
K KLL+ + DE D L + + R K+ + + +++E+ GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367
BLAST of CSPI05G11470 vs. Swiss-Prot
Match:
TRM1_THEON (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) GN=trm1 PE=3 SV=1)
HSP 1 Score: 138.3 bits (347), Expect = 2.2e-31
Identity = 115/373 (30.83%), Postives = 183/373 (49.06%), Query Frame = 1
Query: 64 FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 123
F+ A RD+ VLA K+ G RVLDAL GIR +RY +E+ A + ND N
Sbjct: 27 FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86
Query: 124 NRRVIVKNLS-----------RVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVD 183
R+IV N+ ++A G+ + +VTN DANR+M E + +FDF+D+D
Sbjct: 87 AFRLIVDNVKLNFSGELEIEGKMAVLEGE-KTLVVTNADANRLMNEQFR---YFDFVDLD 146
Query: 184 SFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYVRPMPFSNEIGL 243
FGS FL A+ A+K G+L +T+TD G G +R + L Y A +E GL
Sbjct: 147 PFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGL 206
Query: 244 RMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGN 303
R+LIG + R A+ L +YY H FR L++ G ++ + Y +
Sbjct: 207 RILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ--- 266
Query: 304 SQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQG 363
E G+ ++ + GPLW+GPL + +++E++ L+ + +G+
Sbjct: 267 ------DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS--- 326
Query: 364 KDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIAS 423
K +K L + DE + +P Y VA R + L+ ++ + + GY A+R+H +
Sbjct: 327 KKTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSP 370
Query: 424 NAIKTNCPMAECI 425
A+KT+ P E +
Sbjct: 387 TALKTDAPFEEVL 370
BLAST of CSPI05G11470 vs. Swiss-Prot
Match:
TRM1_PYRFU (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) GN=trm1 PE=1 SV=1)
HSP 1 Score: 136.3 bits (342), Expect = 8.3e-31
Identity = 110/369 (29.81%), Postives = 172/369 (46.61%), Query Frame = 1
Query: 64 FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 123
F+ A RD+ VL L K ++ VLDAL GIR +R+ +E+ A+ + ND N+
Sbjct: 29 FYNPRMAPNRDVVVL---LLNVLKPKI-VLDALSATGIRGIRFALETPAEEIWMNDINEL 88
Query: 124 NRRVIVKNL-----SRVAEN-----IGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
++ KN+ + EN + ++ N DANR+M E K +F FID+D
Sbjct: 89 AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148
Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 243
FGS FL A+ ++K G+L VT+TDG G P L Y A +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208
Query: 244 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN---RHYGFVSYCEKCG 303
L+G +AR A+ L +YY H FR +++ G + + G+V + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268
Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 363
+ S +S GPLW+GPL + + ++L L + N
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328
Query: 364 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 420
+++ KLL + +E D L + + R K+ L ++ E+ GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370
BLAST of CSPI05G11470 vs. Swiss-Prot
Match:
TRM1_SULSO (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=trm1 PE=3 SV=1)
HSP 1 Score: 135.2 bits (339), Expect = 1.9e-30
Identity = 106/361 (29.36%), Postives = 189/361 (52.35%), Query Frame = 1
Query: 64 FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVES-EADFVLANDAND 123
F+ + RDL V+ ++ + ++DAL GIR +RY VES +++ ++ ND N
Sbjct: 35 FYNPKMTFNRDLSVIVVSILKPKI----IVDALSATGIRGIRYYVESWKSEQLILNDKNP 94
Query: 124 ENRRVIVKNLSRVAENIG-DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFL 183
+I N A+N G +N + + N DAN ++ E D+ID+D FGS + F+
Sbjct: 95 NATSLIQIN----AKNNGIENAK--IYNKDANALLYEVKS-----DYIDIDPFGSPAPFI 154
Query: 184 RPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVARE 243
++NA +G++ T+TD G P+ Y A + S E+GLR+LIG + RE
Sbjct: 155 LSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIRE 214
Query: 244 ASVLGFYATPLFSYYSYHGPVFRVMLRINRGR----IHKNRHYGFVSYCEKCGNSQAFSW 303
A++L PLFS+Y+ + +R+ +R+ G + N+H + C +CG Q F
Sbjct: 215 AAILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG-FQTFV- 274
Query: 304 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKL 363
E + CP E + GPL+IGPL+S ++++ I+ L + ++ + ++KL
Sbjct: 275 EENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKL 334
Query: 364 LRQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLSTMMSEMIKAGYAASRSHIASNAIKT 418
L V E +++ + + ++AS+ K+++ PP+ +++ + ASR+H A I+T
Sbjct: 335 LN--VIEKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRT 364
BLAST of CSPI05G11470 vs. Swiss-Prot
Match:
TRM1_SULIY (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1) GN=trm1 PE=3 SV=1)
HSP 1 Score: 128.6 bits (322), Expect = 1.7e-28
Identity = 110/374 (29.41%), Postives = 189/374 (50.53%), Query Frame = 1
Query: 64 FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVES-EADFVLANDAND 123
F+ + RDL V+ +L + +LDAL GIR +RY VES +++ ++ ND N
Sbjct: 35 FYNPKMTFNRDLSVIVVSLLKPKI----ILDALSATGIRGIRYYVESWKSEQLILNDKNS 94
Query: 124 ENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLR 183
+I N V N +N + + N DAN ++ E ++ID+D FGS F+
Sbjct: 95 TAASLIQIN---VKNNGIENAK--IYNKDANALLYEIKS-----EYIDIDPFGSPVPFIL 154
Query: 184 PAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREA 243
++NA +G++ T+TD G + Y A + S E+GLR+LIG + REA
Sbjct: 155 SSVNATIRNGIVAFTATDLSPLEGSSRTSCRRKYDAINYKLSSSKELGLRILIGKIIREA 214
Query: 244 SVLGFYATPLFSYYS-YHGPVFRVM---LRINRGRIHKNRHYGFVSYCEKCGNSQAFSWS 303
+ L PLFS+Y+ Y+ +F ++ R I+KN Y C +CG Q F
Sbjct: 215 ATLEKTVHPLFSFYADYYYRLFAIVESGARKADENINKNLKY--FGECPRCG-FQTFV-D 274
Query: 304 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 363
E + CP E+ ++ GPL+IGPLH+ ++++ ++ + ++ + ++KLL
Sbjct: 275 ENCKTKCPI----CGENFIIIGPLYIGPLHNMEFLKRMIDTYSDFNYLSS--FNRIQKLL 334
Query: 364 RQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLSTMMSEMIKAGYAASRSHIASNAIKTN 423
V E ++K + + ++AS+ KV++ PP+ +++ + AS++H A I+T+
Sbjct: 335 N--VIEKEAKYKSVFYNISKLASKLKVSAIPPIDSILECL----GDASKTHFAPTGIRTD 378
Query: 424 CPMAECIRIAQLQR 432
E IR + R
Sbjct: 395 KGYEEIIRCVKSLR 378
BLAST of CSPI05G11470 vs. TrEMBL
Match:
A0A0A0KP99_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G220920 PE=4 SV=1)
HSP 1 Score: 886.7 bits (2290), Expect = 1.2e-254
Identity = 438/438 (100.00%), Postives = 438/438 (100.00%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI
Sbjct: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIAQLQRNCLGAHQ 439
AECIRIAQLQRNCLGAHQ
Sbjct: 421 AECIRIAQLQRNCLGAHQ 438
BLAST of CSPI05G11470 vs. TrEMBL
Match:
M5XJK8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005915mg PE=4 SV=1)
HSP 1 Score: 620.5 bits (1599), Expect = 1.6e-174
Identity = 307/438 (70.09%), Postives = 358/438 (81.74%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPL--NNCLSNNRTERGLDF 60
M+T+ P+TLSP FL P S + Q+S SN P +NC S +TERGL F
Sbjct: 1 MITLTPKTLSPFSFLCNPIPKHQNQNSKYTIVTQYSDSNSKPTFNSNCESEFQTERGLKF 60
Query: 61 DIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLAN 120
DIG+TFFRHESATGRDLGVLAAALY++SKG+LRVLDALCGCGIRSLRYL+E+EADFVLAN
Sbjct: 61 DIGETFFRHESATGRDLGVLAAALYKRSKGKLRVLDALCGCGIRSLRYLIEAEADFVLAN 120
Query: 121 DANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
DAND+NR+VI +NLS+V GD+ RW VT DANR+MTE YLK+DFFD ID+DSFGSDS
Sbjct: 121 DANDDNRKVITENLSQVES--GDDPRWAVTLSDANRIMTECYLKRDFFDLIDIDSFGSDS 180
Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGV 240
SFLR AINALKLDGLLYVTSTDGFSSGG RP Q+LAAYGAYVRPMP+SNE+GLRMLIGG
Sbjct: 181 SFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRMLIGGA 240
Query: 241 AREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWS 300
R ASVLG+ TPLFSYYSYHGP+FRVMLR+NRG++ NR+YGF+SYC CG+SQ +SW+
Sbjct: 241 VRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQEYSWA 300
Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 360
ELG+MSCPC+DS+VS+SL+VSGPLW GPLH YI ++L LA++W W G+G G DL+KLL
Sbjct: 301 ELGRMSCPCSDSKVSKSLIVSGPLWTGPLHDTIYITEMLHLAEEWGWAGSGSGTDLDKLL 360
Query: 361 RQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNC 420
QMV ESD +LP GY K+DEVASRAKVNSPPL +MS + K GYAASRSHI SNAIKTNC
Sbjct: 361 NQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAIKTNC 420
Query: 421 PMAECIRIAQLQRNCLGA 437
MA CIRIA+ + C A
Sbjct: 421 SMAGCIRIAKQLQQCSSA 436
BLAST of CSPI05G11470 vs. TrEMBL
Match:
A5ACL3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g02690 PE=4 SV=1)
HSP 1 Score: 598.6 bits (1542), Expect = 6.6e-168
Identity = 298/433 (68.82%), Postives = 356/433 (82.22%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
M+ ++ +TLS SPFL +++ H + L S S +P S RTERGL FD
Sbjct: 1 MILLSAKTLSSSPFLL--RSSPHPQNPNPKPL---SASQALPK----SEYRTERGLTFDS 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
G++FFRHESATGRDLGVLAA+LY+KSKG +RVLDA+CGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61 GNSFFRHESATGRDLGVLAASLYKKSKGSVRVLDAMCGCGIRSLRYLVEAEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
N+ R I++NLS+ G+ RRW+VT+ DANRVMTE YL++DF+D IDVDSFGS+SSF
Sbjct: 121 NENYGRTILENLSQGLGGSGEERRWVVTHTDANRVMTECYLRRDFYDLIDVDSFGSESSF 180
Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
LR A NALKLDGL YVTSTDG++SGGHRP SLA+YGA+VRPMP+SNE+GLRMLIGG R
Sbjct: 181 LRSAYNALKLDGLFYVTSTDGYTSGGHRPHHSLASYGAFVRPMPYSNEVGLRMLIGGALR 240
Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
EASVLG+ TPLFSYYSYHGPVFRVMLR+NRG++ +NR+YGF+SYC KCGNSQAFSW+EL
Sbjct: 241 EASVLGYRITPLFSYYSYHGPVFRVMLRMNRGKLPENRNYGFISYCTKCGNSQAFSWNEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
G++SCPC+D++VS SLVVSGPLWIGPLH+A Y+ ++L LA+QW W GN L KLL+Q
Sbjct: 301 GRISCPCSDAKVSSSLVVSGPLWIGPLHNASYLTEMLNLAEQWGWTGNDTETHLGKLLKQ 360
Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
M+DESD KLP GYIK+DEVASRAK+NSPPL TMMS + K GYA SRSHIASNAIKTNCPM
Sbjct: 361 MIDESDPKLPFGYIKLDEVASRAKINSPPLRTMMSALNKEGYAVSRSHIASNAIKTNCPM 420
Query: 421 AECIRIAQLQRNC 434
A CIRIA+ + C
Sbjct: 421 AVCIRIAKELQQC 424
BLAST of CSPI05G11470 vs. TrEMBL
Match:
B9I878_POPTR (N2 family protein OS=Populus trichocarpa GN=POPTR_0013s09180g PE=4 SV=1)
HSP 1 Score: 592.4 bits (1526), Expect = 4.7e-166
Identity = 294/431 (68.21%), Postives = 350/431 (81.21%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNN--CLSNNRTERGLDF 60
MLT++P+ +PFL+ PQ + + I + K +N C S ++ERGL+F
Sbjct: 1 MLTLSPK----APFLYKPQNPN---PNPKIQHY------KPQINGIYCKSELQSERGLEF 60
Query: 61 DIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLAN 120
+ G+TFFRHESA GRDLGVLAA+LY +SKG+LRVLDALCGCGIRSLRYL+E++ADFVLAN
Sbjct: 61 ETGETFFRHESAKGRDLGVLAASLYNQSKGKLRVLDALCGCGIRSLRYLMEAKADFVLAN 120
Query: 121 DANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
DANDE+RRVI++NL RV GD R+W+VT+ DANRV++E YL+KDFFD ID+DSFGSD
Sbjct: 121 DANDEHRRVILENLKRVDRGFGDERKWVVTHLDANRVLSECYLQKDFFDLIDIDSFGSDI 180
Query: 181 S-FLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGG 240
S FLR A+N L DGLLYVTSTDG SSGGH P SLAAYGAYVRPMP+SNE+GLRMLIGG
Sbjct: 181 SLFLRSAMNTLSFDGLLYVTSTDGHSSGGHHPDHSLAAYGAYVRPMPYSNEVGLRMLIGG 240
Query: 241 VAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSW 300
REASVLG+Y TPLFSYYSYHGPVFRVMLR+NRG++ +NRHYGF++YC CGNSQ FSW
Sbjct: 241 AVREASVLGYYITPLFSYYSYHGPVFRVMLRVNRGKLLENRHYGFITYCNNCGNSQEFSW 300
Query: 301 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKL 360
+ELGQ+SC C+ S S SLV+SGPLW GPLHSA YI ++ LA++W WIGNG DLEKL
Sbjct: 301 AELGQISCSCSGSHGSRSLVISGPLWTGPLHSAAYISKMINLAEEWGWIGNGAETDLEKL 360
Query: 361 LRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTN 420
L+QM+DESD +L GYIKMDEVASRAK+NSPPL TMMS M K GYA SRSHIASNAIKT+
Sbjct: 361 LKQMLDESDPRLKSGYIKMDEVASRAKINSPPLRTMMSAMQKEGYATSRSHIASNAIKTD 418
Query: 421 CPMAECIRIAQ 429
CPM+ CIRI++
Sbjct: 421 CPMSVCIRISK 418
BLAST of CSPI05G11470 vs. TrEMBL
Match:
A0A061FZG2_THECC (N2,N2-dimethylguanosine tRNA methyltransferase isoform 1 OS=Theobroma cacao GN=TCM_045543 PE=4 SV=1)
HSP 1 Score: 590.1 bits (1520), Expect = 2.3e-165
Identity = 288/440 (65.45%), Postives = 359/440 (81.59%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
MLT P+TLSPSPF+H N + ++S L N L C N TERG+ FD
Sbjct: 1 MLTTNPKTLSPSPFIHKTLINPKYSSTSPKFL------NFNSLIRCKCNQVTERGILFDT 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
G+TFFR ESA GRDLGVL+AALY++SKG+LRVLDA+CGCGIRSLRYLVES+ADFVLANDA
Sbjct: 61 GETFFRQESAIGRDLGVLSAALYKQSKGQLRVLDAMCGCGIRSLRYLVESKADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
N+ +RRVI++NL++V G+ +RW+VT+C+ANRV+T+ YL++D+FDFID+DSFGSDSSF
Sbjct: 121 NESHRRVILENLAQVERFQGEKKRWVVTHCEANRVLTDCYLQRDYFDFIDIDSFGSDSSF 180
Query: 181 -LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVA 240
LR A ++LKLDGL+YVTSTDG+SSGGHRP SLAAYGAYVRPMP++NE+GLR+LIGG
Sbjct: 181 FLRAAFSSLKLDGLVYVTSTDGYSSGGHRPFHSLAAYGAYVRPMPYANELGLRILIGGAV 240
Query: 241 REASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN------RHYGFVSYCEKCGNSQ 300
REASVLG++ TPLFSYYSYHGPVFRV+LR+NRG++ N R YGF+ YC +CGNSQ
Sbjct: 241 REASVLGYHVTPLFSYYSYHGPVFRVLLRMNRGKLPDNSFHINDRDYGFICYCYRCGNSQ 300
Query: 301 AFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKD 360
A SWSELG++ CPC++S+ + SLVVSGP+W GPLHSA YI ++L LA+QW W+GNG G D
Sbjct: 301 AVSWSELGRIRCPCSNSKDAASLVVSGPMWTGPLHSAAYIMEMLNLAEQWGWVGNGAGTD 360
Query: 361 LEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNA 420
LEKLL++MV+ESD +LP GYIK+DEVA RA+ N+P + T+M+ + K GYAASRSHIASNA
Sbjct: 361 LEKLLKRMVEESDPRLPFGYIKLDEVACRAQTNTPSIRTIMNSLHKEGYAASRSHIASNA 420
Query: 421 IKTNCPMAECIRIAQLQRNC 434
IKT+CPMA CIRIA++ C
Sbjct: 421 IKTDCPMAGCIRIAKILHGC 434
BLAST of CSPI05G11470 vs. TAIR10
Match:
AT3G56330.1 (AT3G56330.1 N2,N2-dimethylguanosine tRNA methyltransferase)
HSP 1 Score: 557.4 bits (1435), Expect = 8.5e-159
Identity = 268/430 (62.33%), Postives = 338/430 (78.60%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCL--SNNRTERGLDF 60
+LT++P+TLS S F + ++ + S F+ S K + L S + ER L+F
Sbjct: 2 LLTLSPKTLSSSSFFTVHKSQNPKCKSPDSCRFK---SLKCSFDRILVKSEVQIERNLEF 61
Query: 61 DIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLAN 120
+ G+TFFRHESA GRDLGVL+A LY++S G LRVLDA+CGCGIRSLRYLVE+EADFV+AN
Sbjct: 62 ETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMAN 121
Query: 121 DANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
DAND+NRRVI NLS+V GD RRW+VT+ AN+ M E Y+ DFFD ID+DSFGSDS
Sbjct: 122 DANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDS 181
Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGV 240
SFLR A NAL+L GLLY+TSTDG+SSGGHRP SLAAYGA++RPMPF NEIGLRMLIGG
Sbjct: 182 SFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGA 241
Query: 241 AREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWS 300
REA++LG++ TPLFSYYSYHGPVFRVMLR++RG++H++R+YGFV++C CG+S +
Sbjct: 242 VREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFD 301
Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 360
ELG M CPC+D++ S SLVVSGP+W+GPLH A Y+ ++L LAK+W W+ G G DL+KLL
Sbjct: 302 ELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLL 361
Query: 361 RQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNC 420
M++ESD +LP GY KMDE+ASRAK+NSPPL MMS ++K GYAASRSHI NA+KT+C
Sbjct: 362 SIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDC 421
Query: 421 PMAECIRIAQ 429
PM+ +RIA+
Sbjct: 422 PMSHFVRIAK 428
BLAST of CSPI05G11470 vs. TAIR10
Match:
AT5G15810.1 (AT5G15810.1 N2,N2-dimethylguanosine tRNA methyltransferase)
HSP 1 Score: 92.8 bits (229), Expect = 6.0e-19
Identity = 96/390 (24.62%), Postives = 161/390 (41.28%), Query Frame = 1
Query: 59 DIGDTFFRHESATGRDLGVLAAALYRKSKGRL---RVLDALCGCGIRSLRYLVESEA-DF 118
D G T HE T A Y ++ L RVL+AL G+R+LRY E E
Sbjct: 186 DNGKTNGEHEVTTQDGPKEAAKTAYESARRELKPPRVLEALSASGLRALRYAREVEGIGQ 245
Query: 119 VLANDANDENRRVIVKNL--------SRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFF 178
V+A D + + +N+ S+V ++ D R ++++ KD F
Sbjct: 246 VVALDNDPASVEACQRNIKFNGLMSTSKVESHLTDARVHMLSH------------PKD-F 305
Query: 179 DFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFS 238
D +D+D +G+ S FL A+ ++ GLL T+TD G + YG+Y +
Sbjct: 306 DVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVLCGANGEVCYSKYGSYPLKGKYC 365
Query: 239 NEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYC 298
+E+ LR+L+ + A+ Y P+ S RV +R+ +SY
Sbjct: 366 HEMALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVRVYTSASAMKNTPLKLSYV 425
Query: 299 EKCGNSQAFSWSELGQMSCPCNDS----------------RVSESLVVSGPLWIGPLHSA 358
+C +F +G+ S P N+S + + GP+W P+H
Sbjct: 426 YQCIGCDSFHLQSVGR-SLPKNNSVRYLPGVGPVVPQDCTHCGKKYNMGGPIWSAPIHDQ 485
Query: 359 DYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPL 418
+++ IL K + D + + E +P+ ++ + +++ K SP
Sbjct: 486 EWVNSILNGVKSMK--DRYPAYDRICAVLTTISEELPDVPL-FLSLHSLSATLKCTSPSA 545
Query: 419 STMMSEMIKAGYAASRSHIASNAIKTNCPM 421
+ S +I A Y S SH+ IKT+ PM
Sbjct: 546 ALFRSAVINAKYRVSGSHVNPLGIKTDAPM 556
BLAST of CSPI05G11470 vs. TAIR10
Match:
AT3G02320.1 (AT3G02320.1 N2,N2-dimethylguanosine tRNA methyltransferase)
HSP 1 Score: 87.4 bits (215), Expect = 2.5e-17
Identity = 82/348 (23.56%), Postives = 143/348 (41.09%), Query Frame = 1
Query: 91 RVLDALCGCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNC 150
+VL+AL G+R+LRY E E + ND + ++ NI N ++
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDND------LASVEACQRNIKFNGSVAISKV 182
Query: 151 DANRVMTEFYL--KKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHR 210
+++ ++ FD +D+D +GS S FL AI ++ GLL T+TD G
Sbjct: 183 ESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVLCGGN 242
Query: 211 PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLR 270
+ YG+Y + +E+ LR+L+ + A+ Y P+ S RV +R
Sbjct: 243 GEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVR 302
Query: 271 INRGRIHKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDS----------------RV 330
+ +SY +C +F +G+ S P N+S
Sbjct: 303 VYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVVKQDCNHC 362
Query: 331 SESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSKLPVG 390
+ + GP+W P+H +++ IL K + D + V E +P+
Sbjct: 363 GKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK--DRYPAYDRISAVLTTVSEELLDVPL- 422
Query: 391 YIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
++ + + + K SP + S +I A Y S +H+ +KT+ PM
Sbjct: 423 FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458
BLAST of CSPI05G11470 vs. NCBI nr
Match:
gi|778701515|ref|XP_011655038.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus])
HSP 1 Score: 886.7 bits (2290), Expect = 1.7e-254
Identity = 438/438 (100.00%), Postives = 438/438 (100.00%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI
Sbjct: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIAQLQRNCLGAHQ 439
AECIRIAQLQRNCLGAHQ
Sbjct: 421 AECIRIAQLQRNCLGAHQ 438
BLAST of CSPI05G11470 vs. NCBI nr
Match:
gi|659114163|ref|XP_008456934.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo])
HSP 1 Score: 847.8 bits (2189), Expect = 9.0e-243
Identity = 418/438 (95.43%), Postives = 426/438 (97.26%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
MLTVA TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK NNCLSNNRTERGLDFDI
Sbjct: 1 MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIAQLQRNCLGAHQ 439
A+CIRIAQLQ+NCLG HQ
Sbjct: 421 ADCIRIAQLQQNCLGVHQ 438
BLAST of CSPI05G11470 vs. NCBI nr
Match:
gi|659114167|ref|XP_008456937.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Cucumis melo])
HSP 1 Score: 774.2 bits (1998), Expect = 1.3e-220
Identity = 382/399 (95.74%), Postives = 388/399 (97.24%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
MLTVA TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK NNCLSNNRTERGLDFDI
Sbjct: 1 MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 400
M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399
BLAST of CSPI05G11470 vs. NCBI nr
Match:
gi|595999826|ref|XP_007218021.1| (hypothetical protein PRUPE_ppa005915mg [Prunus persica])
HSP 1 Score: 620.5 bits (1599), Expect = 2.3e-174
Identity = 307/438 (70.09%), Postives = 358/438 (81.74%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPL--NNCLSNNRTERGLDF 60
M+T+ P+TLSP FL P S + Q+S SN P +NC S +TERGL F
Sbjct: 1 MITLTPKTLSPFSFLCNPIPKHQNQNSKYTIVTQYSDSNSKPTFNSNCESEFQTERGLKF 60
Query: 61 DIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLAN 120
DIG+TFFRHESATGRDLGVLAAALY++SKG+LRVLDALCGCGIRSLRYL+E+EADFVLAN
Sbjct: 61 DIGETFFRHESATGRDLGVLAAALYKRSKGKLRVLDALCGCGIRSLRYLIEAEADFVLAN 120
Query: 121 DANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
DAND+NR+VI +NLS+V GD+ RW VT DANR+MTE YLK+DFFD ID+DSFGSDS
Sbjct: 121 DANDDNRKVITENLSQVES--GDDPRWAVTLSDANRIMTECYLKRDFFDLIDIDSFGSDS 180
Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGV 240
SFLR AINALKLDGLLYVTSTDGFSSGG RP Q+LAAYGAYVRPMP+SNE+GLRMLIGG
Sbjct: 181 SFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRMLIGGA 240
Query: 241 AREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWS 300
R ASVLG+ TPLFSYYSYHGP+FRVMLR+NRG++ NR+YGF+SYC CG+SQ +SW+
Sbjct: 241 VRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQEYSWA 300
Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 360
ELG+MSCPC+DS+VS+SL+VSGPLW GPLH YI ++L LA++W W G+G G DL+KLL
Sbjct: 301 ELGRMSCPCSDSKVSKSLIVSGPLWTGPLHDTIYITEMLHLAEEWGWAGSGSGTDLDKLL 360
Query: 361 RQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNC 420
QMV ESD +LP GY K+DEVASRAKVNSPPL +MS + K GYAASRSHI SNAIKTNC
Sbjct: 361 NQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAIKTNC 420
Query: 421 PMAECIRIAQLQRNCLGA 437
MA CIRIA+ + C A
Sbjct: 421 SMAGCIRIAKQLQQCSSA 436
BLAST of CSPI05G11470 vs. NCBI nr
Match:
gi|645256266|ref|XP_008233872.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Prunus mume])
HSP 1 Score: 614.8 bits (1584), Expect = 1.3e-172
Identity = 304/438 (69.41%), Postives = 356/438 (81.28%), Query Frame = 1
Query: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPL--NNCLSNNRTERGLDF 60
M+T+ P+TLSP FL P S + Q+S S+ P +NC S +TERGL F
Sbjct: 1 MITLTPKTLSPFSFLCNPIPKHQNQNSKYTIVTQYSDSSSKPTFNSNCESEFQTERGLKF 60
Query: 61 DIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLAN 120
DIG+TFFRHESATGRDLGVLAAALY++SK +LRVLDALCGCGIRSLRYL+E+EADFVLAN
Sbjct: 61 DIGETFFRHESATGRDLGVLAAALYKRSKDKLRVLDALCGCGIRSLRYLIEAEADFVLAN 120
Query: 121 DANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
DAND+N +VI +NLS+V + GD+ RW VT DANR+MTE YLK+DFFD ID+DSFGSDS
Sbjct: 121 DANDDNGKVITENLSQVESSSGDDPRWAVTLLDANRIMTECYLKRDFFDLIDIDSFGSDS 180
Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGV 240
SFLR AINALKLDGLLYVTSTDGFSSGG RP Q+LAAYGAYVRPMP+SNE+GLRMLIGG
Sbjct: 181 SFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRMLIGGA 240
Query: 241 AREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWS 300
R ASVLG+ TPLFSYYSYHGP+FRVMLR+NRG++ NR+YGF+SYC CG+SQ +SW+
Sbjct: 241 VRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQEYSWA 300
Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 360
ELG+MSCPC+DS+VS+SLVVSGPLW GPLH YI ++L LA++W W G+G DL+KLL
Sbjct: 301 ELGRMSCPCSDSKVSKSLVVSGPLWTGPLHDTVYITEMLHLAEEWGWAGSGSRTDLDKLL 360
Query: 361 RQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNC 420
QMV ESD +LP GY K+DEVASRAKVNSPPL +MS + K GYAASRSHI SNAIKTNC
Sbjct: 361 NQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAIKTNC 420
Query: 421 PMAECIRIAQLQRNCLGA 437
MA CIRIA+ + C A
Sbjct: 421 SMAGCIRIAKQLQQCSSA 438
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| TRM1_PYRAB | 1.3e-31 | 28.46 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O... | [more] |
| TRM1_THEON | 2.2e-31 | 30.83 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N... | [more] |
| TRM1_PYRFU | 8.3e-31 | 29.81 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ... | [more] |
| TRM1_SULSO | 1.9e-30 | 29.36 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus solfataricus (strain A... | [more] |
| TRM1_SULIY | 1.7e-28 | 29.41 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus islandicus (strain Y.G... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KP99_CUCSA | 1.2e-254 | 100.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_5G220920 PE=4 SV=1 | [more] |
| M5XJK8_PRUPE | 1.6e-174 | 70.09 | Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005915mg PE=4 SV=1 | [more] |
| A5ACL3_VITVI | 6.6e-168 | 68.82 | Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g02690 PE=4 SV=... | [more] |
| B9I878_POPTR | 4.7e-166 | 68.21 | N2 family protein OS=Populus trichocarpa GN=POPTR_0013s09180g PE=4 SV=1 | [more] |
| A0A061FZG2_THECC | 2.3e-165 | 65.45 | N2,N2-dimethylguanosine tRNA methyltransferase isoform 1 OS=Theobroma cacao GN=T... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G56330.1 | 8.5e-159 | 62.33 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
| AT5G15810.1 | 6.0e-19 | 24.62 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
| AT3G02320.1 | 2.5e-17 | 23.56 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
