Cucsa.144000 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.144000
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionN(2),N(2)-dimethylguanosine tRNA methyltransferase, putative
Locationscaffold01080 : 381996 .. 384915 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGTCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTTTACCGGAAATCCAAGGGCCGTCTTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGATCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTATCTAGAGTTGCGGAAAATATTGGGGACAATCGGCGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGCTTCTCTTCCGGCGGACATCGGCCTTCTCAGTGAGTGCAGAATCTTACTCTTTTAGTTATTTGCTTTAACTGGTTCATTAAAATCTTGATATGAAATATCTTATCATTGTAATATTTTGTATCTATGCATAGAGGTGCTGAGGGATTGATAATGTTTATAATTAAATTTAACAATAACTCAATAGTTTAAGCTCTTGGGTCTTTGAGTGATTAGATATGGTATAGAGTTGATGTGTTTAAACTCTGTAATGTACTAATGTCATCTCCTTCCCACTTAATATTGTTTTTCACTTTTGTTGGGAATTTTATATAAATTTTCAAGAGATAACTGTGGTGGAGTGCTATTGTTATAATTAAATTTATCACAAATTATCAGCTTAAACTTTTTGGGTCAATTATTTAATAAAAAAGATTAGATATAGTTATTTTAGTTTTGATTTTTAGTAGTTGAATTTGGTGTTAGCTCTCAATTAATAGTGTTAGTTACTTGGTGAGTCTTTGTTTCTAGGACTAAGCAATTAGACTTGGAACTGACGGATTGGTTAAACAAAAACTTTAAACTGACTTTTCAGCGGAATGTTTTAAAAAACTGAACTGAACTGAACTAGCTAAATTGACAAAACCAGCTTAAATTGAATAAGGGTTGCTAAAAATAACAAACTTTCTCCAAATCATTCTGATCGTTTCTGGTTTTGACTTGCATCCTTACTTGTACCAAGTTTTAAGCCTAAAAGTGAAATGGTTGCTAAGTATATGAATATAGGTGGATAATTATATGAAGATGTGGTAATTAAAATTCTTTTTTCTCCTTTTTCTTGTTTCTCTCTTTTTCTTTCCATTTCTCTCTAGATCTTTAGCTGCATACGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTCATAAAAACAGGTGATAGTTGATGGGTTTAGCGTTGTCTGCTAGTGTTGGATGTATCTAGTATGATCATTGATGGTGATTGATCGAAGCTCTCACTATTCAATATCAGGCATTATGGTTTTGTTAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTAAGATAACGACTACTATGATTTTGCTTGAAATGATTAATGAATATGCTTGGATTTGGTGGTTTCTTATTCGTTTAGGTTCTTGATTTCTAGTGTAATTACTTGCAACATTCAATCTTACAAGTTCGGAGATACATTAAGTTTTTTGACTTGTTAGGTTTCTGAGTCACTTGTGGTCTCGGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATCGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGAGGCAGATGGTGGATGAGAGCGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCCAATTTTATTTGTTCTTCTCGTGATTTAATATTTTATCTTGTTTTGATCCAATAATGCAAGAAGTGATCTATCAAAGCCCCCCTTCTCTATGCTTAGGTGGCAAGTCGGGCAAAAGTGAATTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGTGAGAATACTGGTACTTAAGATGTGATAAATTGTGTGATGAATGTTATTCCACACGGCTTAACTGGTTTTGTTGTCGTGATATTCTCTTGAATGCTTGAAAATAAAGAAATATTGTTGTATACATGAGACACAAGACAAAAAAACAATGATGGGTTATTCTCTATGAGTAGGACATAGCCATGGCGTGGATGTGCTTTGCTAAAATTTATATGTTTTTGAGCTATATCGTTTTTATACAATTTGAAGTCTGTAAGCTTTGTGTGTATAATCTATTTGAAAAAATAAGTTTGGATAGTTTTGTTTCAATATTTATTATTATTATTTTAACAAATTTTTGACACGTGTCTAACAAGTGCAAAAACTTATACTCAATTTGTTTAACTACTGTCCAGCACATGTCTATTTGTCTTGTGGGTACAAGTATATCACACTTGTTCATCATGTTTTGGAAGTGTCCAATACGCGTTGAACATGGACGATTTGTCCAAACTAGCATGTTTTTTTGCTTGGATCGTAGATGGGTGAAAAGAAAAACTGTTCTCTCTTTTCTCTCTCTTTCTGAGGGTTTTGGTTGTTGGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCAACTGCAGCGAAATTGTCTTGGTGCGCATCAAGTCACATGA

mRNA sequence

GGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGTCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTTTACCGGAAATCCAAGGGCCGTCTTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGATCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTATCTAGAGTTGCGGAAAATATTGGGGACAATCGGCGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGCTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATACGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTCATAAAAACAGGCATTATGGTTTTGTTAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTTGTGGTCTCGGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATCGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGAGGCAGATGGTGGATGAGAGCGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTGAATTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCAACTGCAGCGAAATTGTCTTGGTGCGCATCAAGTCACATGA

Coding sequence (CDS)

GGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGTCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTTTACCGGAAATCCAAGGGCCGTCTTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGATCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTATCTAGAGTTGCGGAAAATATTGGGGACAATCGGCGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGCTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATACGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTCATAAAAACAGGCATTATGGTTTTGTTAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTTGTGGTCTCGGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATCGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGAGGCAGATGGTGGATGAGAGCGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTGAATTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCAACTGCAGCGAAATTGTCTTGGTGCGCATCAAGTCACATGA

Protein sequence

GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIAQLQRNCLGAHQVT*
Homology
[Close]
BLAST of Cucsa.144000 vs. Swiss-Prot
Match: TRM1_PYRAB (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=trm1 PE=3 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 1.1e-31
Identity = 105/369 (28.46%), Postives = 180/369 (48.78%), Query Frame = 1 

Query: 10  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 69
           F+    A  RD+ V+   L    K R+ VLDAL   GIR +R+ +E+ A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVV---LLNVLKPRI-VLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 70  NRRVIVKNL-----SRVAENIG-----DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 129
              ++ KN+       + E+ G       +  +V + DANR+M E   K  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 130 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 189
           FGS   FL  A+ ++K  G+L +T+TDG    G  P   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 190 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN---RHYGFVSYCEKCG 249
           L+G VAR A+        + +YY  H   FR  +++  G    +    + G++ + E  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 250 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 309
                   E+ +   P   +         GP+W+GPL S + +E++L +++Q   +   +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 310 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 366
            K   KLL+ + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

[Close]
BLAST of Cucsa.144000 vs. Swiss-Prot
Match: TRM1_THEON (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) GN=trm1 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.4e-31
Identity = 115/373 (30.83%), Postives = 183/373 (49.06%), Query Frame = 1 

Query: 10  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 69
           F+    A  RD+ VLA     K+ G  RVLDAL   GIR +RY +E+ A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 70  NRRVIVKNLS-----------RVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVD 129
             R+IV N+            ++A   G+ +  +VTN DANR+M E +    +FDF+D+D
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGE-KTLVVTNADANRLMNEQFR---YFDFVDLD 146

Query: 130 SFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYVRPMPFSNEIGL 189
            FGS   FL  A+ A+K  G+L +T+TD G   G +R +  L  Y A        +E GL
Sbjct: 147 PFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGL 206

Query: 190 RMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGN 249
           R+LIG + R A+        L +YY  H   FR  L++  G    ++    + Y  +   
Sbjct: 207 RILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ--- 266

Query: 250 SQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQG 309
                  E G+      ++ +       GPLW+GPL + +++E++  L+ +   +G+   
Sbjct: 267 ------DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS--- 326

Query: 310 KDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIAS 369
           K  +K L  + DE +  +P  Y     VA R  +    L+ ++  + + GY A+R+H + 
Sbjct: 327 KKTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSP 370

Query: 370 NAIKTNCPMAECI 371
            A+KT+ P  E +
Sbjct: 387 TALKTDAPFEEVL 370

[Close]
BLAST of Cucsa.144000 vs. Swiss-Prot
Match: TRM1_PYRFU (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) GN=trm1 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 6.9e-31
Identity = 110/369 (29.81%), Postives = 172/369 (46.61%), Query Frame = 1 

Query: 10  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 69
           F+    A  RD+ VL   L    K ++ VLDAL   GIR +R+ +E+ A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVL---LLNVLKPKI-VLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 70  NRRVIVKNL-----SRVAEN-----IGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 129
              ++ KN+       + EN         +  ++ N DANR+M E   K  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 130 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 189
           FGS   FL  A+ ++K  G+L VT+TDG    G  P   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 190 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN---RHYGFVSYCEKCG 249
           L+G +AR A+        L +YY  H   FR  +++  G    +    + G+V + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 250 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 309
             +                S +S      GPLW+GPL +   + ++L L      + N  
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 310 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 366
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

[Close]
BLAST of Cucsa.144000 vs. Swiss-Prot
Match: TRM1_SULSO (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=trm1 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.0e-30
Identity = 106/361 (29.36%), Postives = 189/361 (52.35%), Query Frame = 1 

Query: 10  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVES-EADFVLANDAND 69
           F+  +    RDL V+  ++ +       ++DAL   GIR +RY VES +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPKI----IVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 70  ENRRVIVKNLSRVAENIG-DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFL 129
               +I  N    A+N G +N +  + N DAN ++ E        D+ID+D FGS + F+
Sbjct: 95  NATSLIQIN----AKNNGIENAK--IYNKDANALLYEVKS-----DYIDIDPFGSPAPFI 154

Query: 130 RPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVARE 189
             ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + RE
Sbjct: 155 LSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIRE 214

Query: 190 ASVLGFYATPLFSYYSYHGPVFRVMLRINRGR----IHKNRHYGFVSYCEKCGNSQAFSW 249
           A++L     PLFS+Y+ +   +R+ +R+  G      + N+H  +   C +CG  Q F  
Sbjct: 215 AAILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG-FQTFV- 274

Query: 250 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKL 309
            E  +  CP       E   + GPL+IGPL+S ++++ I+ L   + ++ +     ++KL
Sbjct: 275 EENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKL 334

Query: 310 LRQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLSTMMSEMIKAGYAASRSHIASNAIKT 364
           L   V E +++    +  + ++AS+ K+++ PP+ +++  +      ASR+H A   I+T
Sbjct: 335 LN--VIEKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRT 364

[Close]
BLAST of Cucsa.144000 vs. Swiss-Prot
Match: TRM1_SULIK (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus islandicus (strain M.16.4 / Kamchatka #3) GN=trm1 PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.4e-28
Identity = 110/376 (29.26%), Postives = 189/376 (50.27%), Query Frame = 1 

Query: 10  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVES-EADFVLANDAND 69
           F+  +    RDL V+  +L +       +LDAL   GIR +RY VES +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSLLKPKI----ILDALSATGIRGIRYYVESWKSEQLILNDKNS 94

Query: 70  ENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLR 129
               +I  N   V  N  +N +  + N DAN ++ E        ++ID+D FGS   F+ 
Sbjct: 95  TAASLIQIN---VKNNGIENAK--IYNKDANALLYEIKS-----EYIDIDPFGSPVPFIL 154

Query: 130 PAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREA 189
            +INA   +G++  T+TD     G   +     Y A    +  S E+GLR+LIG + REA
Sbjct: 155 SSINATIRNGIVAFTATDLSPLEGSSRTSCRRKYDAINYKLSSSKELGLRILIGKIIREA 214

Query: 190 SVLGFYATPLFSYYSYHGPVFRVMLRINRG------RIHKNRHYGFVSYCEKCGNSQAFS 249
           + L     PLFS+Y+ +   +R+ + +  G       I+KN  Y     C +CG  Q F 
Sbjct: 215 ATLEKTVHPLFSFYADY--YYRLFVIVESGARKADENINKNLKY--FGECPRCG-FQTFV 274

Query: 250 WSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEK 309
             E  +  CP       E+ ++ GPL+IGPLH+ ++++ ++     + ++ +     ++K
Sbjct: 275 -DENCKTKCPI----CGENFIIIGPLYIGPLHNMEFLKRMIDTYSDFNYLSS--FNRIQK 334

Query: 310 LLRQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLSTMMSEMIKAGYAASRSHIASNAIK 369
           LL   V E ++K    +  + ++AS+ KV++ PP+ +++  +      AS++H A   I+
Sbjct: 335 LLN--VIEKEAKYKSVFYNISKLASKLKVSAIPPIDSILECL----GDASKTHFAPTGIR 378

Query: 370 TNCPMAECIRIAQLQR 378
           T+    E IR  +  R
Sbjct: 395 TDKGYEEIIRCVKSLR 378

[Close]
BLAST of Cucsa.144000 vs. TrEMBL
Match: A0A0A0KP99_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G220920 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 1.6e-223
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF
Sbjct: 55  GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 114

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF
Sbjct: 115 VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 174

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML
Sbjct: 175 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 234

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA
Sbjct: 235 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 294

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL
Sbjct: 295 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 354

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 360
           EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI
Sbjct: 355 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 414

Query: 361 KTNCPMAECIRIAQLQRNCLGAHQVT 387
           KTNCPMAECIRIAQLQRNCLGAHQVT
Sbjct: 415 KTNCPMAECIRIAQLQRNCLGAHQVT 440

[Close]
BLAST of Cucsa.144000 vs. TrEMBL
Match: M5XJK8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005915mg PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 2.3e-166
Identity = 285/382 (74.61%), Postives = 329/382 (86.13%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GL FDIG+TFFRHESATGRDLGVLAAALY++SKG+LRVLDALCGCGIRSLRYL+E+EADF
Sbjct: 57  GLKFDIGETFFRHESATGRDLGVLAAALYKRSKGKLRVLDALCGCGIRSLRYLIEAEADF 116

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDAND+NR+VI +NLS+V    GD+ RW VT  DANR+MTE YLK+DFFD ID+DSF
Sbjct: 117 VLANDANDDNRKVITENLSQVES--GDDPRWAVTLSDANRIMTECYLKRDFFDLIDIDSF 176

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GSDSSFLR AINALKLDGLLYVTSTDGFSSGG RP Q+LAAYGAYVRPMP+SNE+GLRML
Sbjct: 177 GSDSSFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRML 236

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGG  R ASVLG+  TPLFSYYSYHGP+FRVMLR+NRG++  NR+YGF+SYC  CG+SQ 
Sbjct: 237 IGGAVRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQE 296

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           +SW+ELG+MSCPC+DS+VS+SL+VSGPLW GPLH   YI ++L LA++W W G+G G DL
Sbjct: 297 YSWAELGRMSCPCSDSKVSKSLIVSGPLWTGPLHDTIYITEMLHLAEEWGWAGSGSGTDL 356

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 360
           +KLL QMV ESD +LP GY K+DEVASRAKVNSPPL  +MS + K GYAASRSHI SNAI
Sbjct: 357 DKLLNQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAI 416

Query: 361 KTNCPMAECIRIAQLQRNCLGA 383
           KTNC MA CIRIA+  + C  A
Sbjct: 417 KTNCSMAGCIRIAKQLQQCSSA 436

[Close]
BLAST of Cucsa.144000 vs. TrEMBL
Match: A5ACL3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g02690 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 5.6e-165
Identity = 280/379 (73.88%), Postives = 329/379 (86.81%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GL FD G++FFRHESATGRDLGVLAA+LY+KSKG +RVLDA+CGCGIRSLRYLVE+EADF
Sbjct: 46  GLTFDSGNSFFRHESATGRDLGVLAASLYKKSKGSVRVLDAMCGCGIRSLRYLVEAEADF 105

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDAN+   R I++NLS+     G+ RRW+VT+ DANRVMTE YL++DF+D IDVDSF
Sbjct: 106 VLANDANENYGRTILENLSQGLGGSGEERRWVVTHTDANRVMTECYLRRDFYDLIDVDSF 165

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GS+SSFLR A NALKLDGL YVTSTDG++SGGHRP  SLA+YGA+VRPMP+SNE+GLRML
Sbjct: 166 GSESSFLRSAYNALKLDGLFYVTSTDGYTSGGHRPHHSLASYGAFVRPMPYSNEVGLRML 225

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGG  REASVLG+  TPLFSYYSYHGPVFRVMLR+NRG++ +NR+YGF+SYC KCGNSQA
Sbjct: 226 IGGALREASVLGYRITPLFSYYSYHGPVFRVMLRMNRGKLPENRNYGFISYCTKCGNSQA 285

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           FSW+ELG++SCPC+D++VS SLVVSGPLWIGPLH+A Y+ ++L LA+QW W GN     L
Sbjct: 286 FSWNELGRISCPCSDAKVSSSLVVSGPLWIGPLHNASYLTEMLNLAEQWGWTGNDTETHL 345

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 360
            KLL+QM+DESD KLP GYIK+DEVASRAK+NSPPL TMMS + K GYA SRSHIASNAI
Sbjct: 346 GKLLKQMIDESDPKLPFGYIKLDEVASRAKINSPPLRTMMSALNKEGYAVSRSHIASNAI 405

Query: 361 KTNCPMAECIRIAQLQRNC 380
           KTNCPMA CIRIA+  + C
Sbjct: 406 KTNCPMAVCIRIAKELQQC 424

[Close]
BLAST of Cucsa.144000 vs. TrEMBL
Match: B9I878_POPTR (N2 family protein OS=Populus trichocarpa GN=POPTR_0013s09180g PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 1.8e-163
Identity = 278/375 (74.13%), Postives = 323/375 (86.13%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GL+F+ G+TFFRHESA GRDLGVLAA+LY +SKG+LRVLDALCGCGIRSLRYL+E++ADF
Sbjct: 44  GLEFETGETFFRHESAKGRDLGVLAASLYNQSKGKLRVLDALCGCGIRSLRYLMEAKADF 103

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDANDE+RRVI++NL RV    GD R+W+VT+ DANRV++E YL+KDFFD ID+DSF
Sbjct: 104 VLANDANDEHRRVILENLKRVDRGFGDERKWVVTHLDANRVLSECYLQKDFFDLIDIDSF 163

Query: 121 GSDSS-FLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 180
           GSD S FLR A+N L  DGLLYVTSTDG SSGGH P  SLAAYGAYVRPMP+SNE+GLRM
Sbjct: 164 GSDISLFLRSAMNTLSFDGLLYVTSTDGHSSGGHHPDHSLAAYGAYVRPMPYSNEVGLRM 223

Query: 181 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQ 240
           LIGG  REASVLG+Y TPLFSYYSYHGPVFRVMLR+NRG++ +NRHYGF++YC  CGNSQ
Sbjct: 224 LIGGAVREASVLGYYITPLFSYYSYHGPVFRVMLRVNRGKLLENRHYGFITYCNNCGNSQ 283

Query: 241 AFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKD 300
            FSW+ELGQ+SC C+ S  S SLV+SGPLW GPLHSA YI  ++ LA++W WIGNG   D
Sbjct: 284 EFSWAELGQISCSCSGSHGSRSLVISGPLWTGPLHSAAYISKMINLAEEWGWIGNGAETD 343

Query: 301 LEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNA 360
           LEKLL+QM+DESD +L  GYIKMDEVASRAK+NSPPL TMMS M K GYA SRSHIASNA
Sbjct: 344 LEKLLKQMLDESDPRLKSGYIKMDEVASRAKINSPPLRTMMSAMQKEGYATSRSHIASNA 403

Query: 361 IKTNCPMAECIRIAQ 375
           IKT+CPM+ CIRI++
Sbjct: 404 IKTDCPMSVCIRISK 418

[Close]
BLAST of Cucsa.144000 vs. TrEMBL
Match: A0A067JFV0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_03334 PE=4 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.3e-158
Identity = 264/374 (70.59%), Postives = 320/374 (85.56%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           G+ F+ G TFF+HESA GRDLGVLAA+LY++SKG LRVLDA+CGCGIRS RYL+E++ADF
Sbjct: 46  GIVFETGGTFFQHESAKGRDLGVLAASLYKQSKGNLRVLDAMCGCGIRSCRYLIEAKADF 105

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDANDE R +I+ NL +V    GD+RRW V++ DANRV+TE YL++DFFD ID+DSF
Sbjct: 106 VLANDANDEYRGIILGNLKKVERGFGDDRRWAVSHFDANRVLTECYLQRDFFDLIDIDSF 165

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GSDS FLR A++ALKLDGLLY+TSTDG+SSGGHRP  SLAAYGAY+RPMP  NEIGLRML
Sbjct: 166 GSDSLFLRSAMSALKLDGLLYLTSTDGYSSGGHRPHCSLAAYGAYIRPMPSCNEIGLRML 225

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGG  REAS+LG++ TPLFSYYSYHGPVFRVML++N+G+ H+NRHYGF+SYC +CGN+Q+
Sbjct: 226 IGGAVREASLLGYHVTPLFSYYSYHGPVFRVMLQVNKGKDHENRHYGFISYCNQCGNTQS 285

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           FSW ELGQ SC CN S  S  + VSGPLW GPLH+A +I ++L LA+QW WIGN  G DL
Sbjct: 286 FSWEELGQSSCSCNSSNDSSPVTVSGPLWTGPLHNATFITEMLNLAEQWGWIGNDVGADL 345

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 360
           +KLL++M+DESD +LP GYIKMDE+ASR K+NSP L TMMS ++K GYAA+RSHIASNAI
Sbjct: 346 DKLLKRMLDESDPRLPFGYIKMDEMASRGKINSPSLKTMMSTLVKKGYAATRSHIASNAI 405

Query: 361 KTNCPMAECIRIAQ 375
           KT+CPM+ CIRIA+
Sbjct: 406 KTDCPMSMCIRIAK 419

[Close]
BLAST of Cucsa.144000 vs. TAIR10
Match: AT3G56330.1 (AT3G56330.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 547.0 bits (1408), Expect = 9.5e-156
Identity = 252/373 (67.56%), Postives = 311/373 (83.38%), Query Frame = 1 

Query: 2   LDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFV 61
           L+F+ G+TFFRHESA GRDLGVL+A LY++S G LRVLDA+CGCGIRSLRYLVE+EADFV
Sbjct: 56  LEFETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFV 115

Query: 62  LANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFG 121
           +ANDAND+NRRVI  NLS+V    GD RRW+VT+  AN+ M E Y+  DFFD ID+DSFG
Sbjct: 116 MANDANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFG 175

Query: 122 SDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLI 181
           SDSSFLR A NAL+L GLLY+TSTDG+SSGGHRP  SLAAYGA++RPMPF NEIGLRMLI
Sbjct: 176 SDSSFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLI 235

Query: 182 GGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAF 241
           GG  REA++LG++ TPLFSYYSYHGPVFRVMLR++RG++H++R+YGFV++C  CG+S   
Sbjct: 236 GGAVREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTL 295

Query: 242 SWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLE 301
            + ELG M CPC+D++ S SLVVSGP+W+GPLH A Y+ ++L LAK+W W+  G G DL+
Sbjct: 296 RFDELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLD 355

Query: 302 KLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIK 361
           KLL  M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+K
Sbjct: 356 KLLSIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALK 415

Query: 362 TNCPMAECIRIAQ 375
           T+CPM+  +RIA+
Sbjct: 416 TDCPMSHFVRIAK 428

[Close]
BLAST of Cucsa.144000 vs. TAIR10
Match: AT5G15810.1 (AT5G15810.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 93.6 bits (231), Expect = 2.9e-19
Identity = 96/390 (24.62%), Postives = 161/390 (41.28%), Query Frame = 1 

Query: 5   DIGDTFFRHESATGRDLGVLAAALYRKSKGRL---RVLDALCGCGIRSLRYLVESEA-DF 64
           D G T   HE  T       A   Y  ++  L   RVL+AL   G+R+LRY  E E    
Sbjct: 186 DNGKTNGEHEVTTQDGPKEAAKTAYESARRELKPPRVLEALSASGLRALRYAREVEGIGQ 245

Query: 65  VLANDANDENRRVIVKNL--------SRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFF 124
           V+A D +  +     +N+        S+V  ++ D R  ++++             KD F
Sbjct: 246 VVALDNDPASVEACQRNIKFNGLMSTSKVESHLTDARVHMLSH------------PKD-F 305

Query: 125 DFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFS 184
           D +D+D +G+ S FL  A+ ++   GLL  T+TD     G       + YG+Y     + 
Sbjct: 306 DVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVLCGANGEVCYSKYGSYPLKGKYC 365

Query: 185 NEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYC 244
           +E+ LR+L+  +   A+    Y  P+ S         RV +R+             +SY 
Sbjct: 366 HEMALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVRVYTSASAMKNTPLKLSYV 425

Query: 245 EKCGNSQAFSWSELGQMSCPCNDS----------------RVSESLVVSGPLWIGPLHSA 304
            +C    +F    +G+ S P N+S                   +   + GP+W  P+H  
Sbjct: 426 YQCIGCDSFHLQSVGR-SLPKNNSVRYLPGVGPVVPQDCTHCGKKYNMGGPIWSAPIHDQ 485

Query: 305 DYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPL 364
           +++  IL   K  +        D    +   + E    +P+ ++ +  +++  K  SP  
Sbjct: 486 EWVNSILNGVKSMK--DRYPAYDRICAVLTTISEELPDVPL-FLSLHSLSATLKCTSPSA 545

Query: 365 STMMSEMIKAGYAASRSHIASNAIKTNCPM 367
           +   S +I A Y  S SH+    IKT+ PM
Sbjct: 546 ALFRSAVINAKYRVSGSHVNPLGIKTDAPM 556

[Close]
BLAST of Cucsa.144000 vs. TAIR10
Match: AT3G02320.1 (AT3G02320.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-17
Identity = 82/348 (23.56%), Postives = 143/348 (41.09%), Query Frame = 1 

Query: 37  RVLDALCGCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNC 96
           +VL+AL   G+R+LRY  E E    +    ND      + ++     NI  N    ++  
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDND------LASVEACQRNIKFNGSVAISKV 182

Query: 97  DANRVMTEFYL--KKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHR 156
           +++      ++      FD +D+D +GS S FL  AI ++   GLL  T+TD     G  
Sbjct: 183 ESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVLCGGN 242

Query: 157 PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLR 216
                + YG+Y     + +E+ LR+L+  +   A+    Y  P+ S         RV +R
Sbjct: 243 GEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVR 302

Query: 217 INRGRIHKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDS----------------RV 276
           +             +SY  +C    +F    +G+ S P N+S                  
Sbjct: 303 VYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVVKQDCNHC 362

Query: 277 SESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSKLPVG 336
            +   + GP+W  P+H  +++  IL   K  +        D    +   V E    +P+ 
Sbjct: 363 GKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK--DRYPAYDRISAVLTTVSEELLDVPL- 422

Query: 337 YIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 367
           ++ +  + +  K  SP  +   S +I A Y  S +H+    +KT+ PM
Sbjct: 423 FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

[Close]
BLAST of Cucsa.144000 vs. NCBI nr
Match: gi|778701515|ref|XP_011655038.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus])

HSP 1 Score: 783.1 bits (2021), Expect = 2.2e-223
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF
Sbjct: 55  GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 114

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF
Sbjct: 115 VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 174

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML
Sbjct: 175 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 234

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA
Sbjct: 235 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 294

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL
Sbjct: 295 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 354

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 360
           EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI
Sbjct: 355 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 414

Query: 361 KTNCPMAECIRIAQLQRNCLGAHQVT 387
           KTNCPMAECIRIAQLQRNCLGAHQVT
Sbjct: 415 KTNCPMAECIRIAQLQRNCLGAHQVT 440

[Close]
BLAST of Cucsa.144000 vs. NCBI nr
Match: gi|659114163|ref|XP_008456934.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 765.4 bits (1975), Expect = 4.8e-218
Identity = 374/386 (96.89%), Postives = 381/386 (98.70%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADF
Sbjct: 55  GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADF 114

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDANDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF
Sbjct: 115 VLANDANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 174

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRML
Sbjct: 175 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRML 234

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGGVAREASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQA
Sbjct: 235 IGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQA 294

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL
Sbjct: 295 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 354

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 360
           EKLL QM+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI
Sbjct: 355 EKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 414

Query: 361 KTNCPMAECIRIAQLQRNCLGAHQVT 387
           KTNCPMA+CIRIAQLQ+NCLG HQVT
Sbjct: 415 KTNCPMADCIRIAQLQQNCLGVHQVT 440

[Close]
BLAST of Cucsa.144000 vs. NCBI nr
Match: gi|659114167|ref|XP_008456937.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 687.2 bits (1772), Expect = 1.7e-194
Identity = 336/345 (97.39%), Postives = 341/345 (98.84%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADF
Sbjct: 55  GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADF 114

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDANDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF
Sbjct: 115 VLANDANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 174

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRML
Sbjct: 175 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRML 234

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGGVAREASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQA
Sbjct: 235 IGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQA 294

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL
Sbjct: 295 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 354

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 346
           EKLL QM+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK
Sbjct: 355 EKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

[Close]
BLAST of Cucsa.144000 vs. NCBI nr
Match: gi|595999826|ref|XP_007218021.1| (hypothetical protein PRUPE_ppa005915mg [Prunus persica])

HSP 1 Score: 593.2 bits (1528), Expect = 3.3e-166
Identity = 285/382 (74.61%), Postives = 329/382 (86.13%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GL FDIG+TFFRHESATGRDLGVLAAALY++SKG+LRVLDALCGCGIRSLRYL+E+EADF
Sbjct: 57  GLKFDIGETFFRHESATGRDLGVLAAALYKRSKGKLRVLDALCGCGIRSLRYLIEAEADF 116

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDAND+NR+VI +NLS+V    GD+ RW VT  DANR+MTE YLK+DFFD ID+DSF
Sbjct: 117 VLANDANDDNRKVITENLSQVES--GDDPRWAVTLSDANRIMTECYLKRDFFDLIDIDSF 176

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GSDSSFLR AINALKLDGLLYVTSTDGFSSGG RP Q+LAAYGAYVRPMP+SNE+GLRML
Sbjct: 177 GSDSSFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRML 236

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGG  R ASVLG+  TPLFSYYSYHGP+FRVMLR+NRG++  NR+YGF+SYC  CG+SQ 
Sbjct: 237 IGGAVRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQE 296

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           +SW+ELG+MSCPC+DS+VS+SL+VSGPLW GPLH   YI ++L LA++W W G+G G DL
Sbjct: 297 YSWAELGRMSCPCSDSKVSKSLIVSGPLWTGPLHDTIYITEMLHLAEEWGWAGSGSGTDL 356

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 360
           +KLL QMV ESD +LP GY K+DEVASRAKVNSPPL  +MS + K GYAASRSHI SNAI
Sbjct: 357 DKLLNQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAI 416

Query: 361 KTNCPMAECIRIAQLQRNCLGA 383
           KTNC MA CIRIA+  + C  A
Sbjct: 417 KTNCSMAGCIRIAKQLQQCSSA 436

[Close]
BLAST of Cucsa.144000 vs. NCBI nr
Match: gi|645256266|ref|XP_008233872.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Prunus mume])

HSP 1 Score: 589.7 bits (1519), Expect = 3.6e-165
Identity = 283/382 (74.08%), Postives = 327/382 (85.60%), Query Frame = 1 

Query: 1   GLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADF 60
           GL FDIG+TFFRHESATGRDLGVLAAALY++SK +LRVLDALCGCGIRSLRYL+E+EADF
Sbjct: 57  GLKFDIGETFFRHESATGRDLGVLAAALYKRSKDKLRVLDALCGCGIRSLRYLIEAEADF 116

Query: 61  VLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSF 120
           VLANDAND+N +VI +NLS+V  + GD+ RW VT  DANR+MTE YLK+DFFD ID+DSF
Sbjct: 117 VLANDANDDNGKVITENLSQVESSSGDDPRWAVTLLDANRIMTECYLKRDFFDLIDIDSF 176

Query: 121 GSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRML 180
           GSDSSFLR AINALKLDGLLYVTSTDGFSSGG RP Q+LAAYGAYVRPMP+SNE+GLRML
Sbjct: 177 GSDSSFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRML 236

Query: 181 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQA 240
           IGG  R ASVLG+  TPLFSYYSYHGP+FRVMLR+NRG++  NR+YGF+SYC  CG+SQ 
Sbjct: 237 IGGAVRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQE 296

Query: 241 FSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDL 300
           +SW+ELG+MSCPC+DS+VS+SLVVSGPLW GPLH   YI ++L LA++W W G+G   DL
Sbjct: 297 YSWAELGRMSCPCSDSKVSKSLVVSGPLWTGPLHDTVYITEMLHLAEEWGWAGSGSRTDL 356

Query: 301 EKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAI 360
           +KLL QMV ESD +LP GY K+DEVASRAKVNSPPL  +MS + K GYAASRSHI SNAI
Sbjct: 357 DKLLNQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAI 416

Query: 361 KTNCPMAECIRIAQLQRNCLGA 383
           KTNC MA CIRIA+  + C  A
Sbjct: 417 KTNCSMAGCIRIAKQLQQCSSA 438

The following BLAST results are available for this feature:
BLAST of Cucsa.144000 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TRM1_PYRAB1.1e-3128.46tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O... [more]
TRM1_THEON2.4e-3130.83tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N... [more]
TRM1_PYRFU6.9e-3129.81tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ... [more]
TRM1_SULSO2.0e-3029.36tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus solfataricus (strain A... [more]
TRM1_SULIK1.4e-2829.26tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus islandicus (strain M.1... [more]
BLAST of Cucsa.144000 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KP99_CUCSA1.6e-223100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G220920 PE=4 SV=1[more]
M5XJK8_PRUPE2.3e-16674.61Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005915mg PE=4 SV=1[more]
A5ACL3_VITVI5.6e-16573.88Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g02690 PE=4 SV=... [more]
B9I878_POPTR1.8e-16374.13N2 family protein OS=Populus trichocarpa GN=POPTR_0013s09180g PE=4 SV=1[more]
A0A067JFV0_JATCU1.3e-15870.59Uncharacterized protein OS=Jatropha curcas GN=JCGZ_03334 PE=4 SV=1[more]
BLAST of Cucsa.144000 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G56330.19.5e-15667.56 N2,N2-dimethylguanosine tRNA methyltransferase[more]
AT5G15810.12.9e-1924.62 N2,N2-dimethylguanosine tRNA methyltransferase[more]
AT3G02320.12.1e-1723.56 N2,N2-dimethylguanosine tRNA methyltransferase[more]
BLAST of Cucsa.144000 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778701515|ref|XP_011655038.1|2.2e-223100.00PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus][more]
gi|659114163|ref|XP_008456934.1|4.8e-21896.89PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo][more]
gi|659114167|ref|XP_008456937.1|1.7e-19497.39PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Cucumis melo][more]
gi|595999826|ref|XP_007218021.1|3.3e-16674.61hypothetical protein PRUPE_ppa005915mg [Prunus persica][more]
gi|645256266|ref|XP_008233872.1|3.6e-16574.08PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002905Trm1
Vocabulary: Molecular Function
TermDefinition
GO:0003723RNA binding
GO:0004809tRNA (guanine-N2-)-methyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0008033tRNA processing
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016556 mRNA modification
biological_process GO:0030488 tRNA methylation
biological_process GO:0008033 tRNA processing
cellular_component GO:0009536 plastid
molecular_function GO:0003723 RNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.144000.1Cucsa.144000.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 1..379
score: 1.7E
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 10..372
score: 7.9
IPR002905tRNA methyltransferase, Trm1PROFILEPS51626SAM_MT_TRM1coord: 1..373
score: 94
NoneNo IPR availablePANTHERPTHR10631:SF2N2, N2-DIMETHYLGUANOSINE TRNA METHYLTRANSFERASES-LIKE PROTEINcoord: 1..379
score: 1.7E