MELO3C020140.2 (gene) Melon (DHL92) v3.6.1

Overview
NameMELO3C020140.2
Typegene
OrganismCucumis melo (Melon (DHL92) v3.6.1)
DescriptiontRNA (guanine(26)-N(2))-dimethyltransferase
Locationchr10 : 13529697 .. 13533741 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCATGAAAATGCAAAATCCAACGATTTCCCGCCATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAGTGAGTGCATAATCTTACTCTTTAGTTATCTGCTTTAACCGGTTCATTAAAATCTTGATATGAAATGTCTTATCATTTTAATGTCCTGTACCTATATATAAAGGTGCTGAAGGATTGATAATGGTTATAATTAAATTTAACAATAACTCAATGTTTAAGCTCTTGGGTCAATGAGTGATTAGATATGGTATAGAGTTGATATGTTTGAGCTGTATCATGTTCTAATGTCCATCTTTTCCCCACTTAATATTGATTTTCACTTTTTTTTTTGAGAATTTCATATAAATTTTCAAGTCGACAAGTGTGGTGGAATATTATTCTTATAATCAAATTTATCATAAATCATCAACTTAAATTCTTGGGTCAATCATTTAATAAAAGGATTTTAAATGATTAAGTATAGTTATTTTAGTTTTGAGTTTTAGTAGTTGAATTTGGTGTTAGCTCCCCATTAATGCTGTTAGTTACTTGTTGAATCTTTGTCTCTAGGACTAAGCAAATCGACTTGTAACTGATAGATTACTTAAAACTGACTTTTTAGCTGAATGTTTTAAGAAATTGAACTGAACTAGCTAAATTGACAAAACCAGCTGAAATTGAATAAGGGTTGCTATTTGCTAAAAATAACAAACTTTATCCAAATCATTTTGATTGTTTTTGGTTTTGACTTCCATCCTTACCTGTACTAAGTTTTAAGCCTAAAAGTGAAATGGTTGCTAAGTATATGAATATAGGTGGATAATTATATATGAAGATGCGGTAATTAAAATTCTTTTTTCTCCTTTTTCTTGTCTCTCTTTTCTTCTTTCCATTTCTCTCTAGATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGTGATAGTTGATGAGTTTAGCTTTGTCTGCTAGTGTTGGATGTATCTAGTATGATCATTGATGGTGATTGATCGAAGCTCTCACTATTCAATATCAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTAAGATTATGACTACTATGACGATGCTTGAAATGATTCATGAATATGCTTGGATTTGCTGTTTTCTTATTCATTTGGGTTCTTGCTTTCTAGTGTATTTACTTGCAACATTCAATCTTGTAAGAGTCTCGAGATACATTAAATTTTTTTGACTTGTTAGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCCAAATTTTATTTATTCTTCTCGTGATTTAATATTTTATCTTTCTTTTTGATCCAATAATGCAAGAAGTGATCTATCAAGGCCCCCCTTCTCTATGCTTAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGTGAGAATACTGGTAGTTAAGATGTGATAAATTGTGTGATGCATATTATTCCACATGGCTCAACTGTTTTTGTTGTTGTGATATTCTCTTGAATGCTTGAAAGTAGAGAAATGTTGTTGTATATATTAAACCACCAATTCACCCAAGAGCTTAAGCTGGTGATTGAAGACAAATTTAATTATATATCACTAACAGTATACATGAGACACAAGGCACAAATACGATAGGACACAAAAACAATGATGGGTTATTCTCTATGAGTAGGAAATGGCCATGGCGTGGATATGATTACTAAAATTTATATGTTTTTAAGCTATATCGTTTTTATTCAATTTGAAGTCTGTAAGCTTTGTGTGTATATATATCTATTTGAAAAATTAAGTTTGGATATGGTTTGCTTCAATATTTATTATTATTATTTTGCATTATCACTATACATATCTAAGTATTAATCTTAACAAATATTTGACACTAGTTGAACAAGTGCAAAAACTTGTATTCAATTTGTTCAACTACTGTCCAACAGTTGTATACTTGTCTTGTGGGTACAAGGATATCACACGTGTTCATCATGTTTTGGAGTGTCCAATAAGCGTCGGACTTGGACGATTTTTCCAAACTATAATGAATTTTTTTTTTTGCTTGGAATGCAGATGCTTGAAAAGAAAACAATTCTCTCTTTTCTCTCTTCTCTCTTTCTGAGGGTTTTGGTTGTGGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGAATGTTGTAGCCATAGCTTGGGGCTATGGAAACTACAACCCTTCATACGCAAACAAGAACGAAATGAAACCAATGTAAGCATCCATCTCTCTTTAGTTATCTCATTTTGAAAATTGATGAAGATAATTATGGGTCAAATGCGTTAAAAGTGGTAAAGAACCATGTCCGATGCTCTCAAGAAATTTGCGTTGCAGGTTTTGGTGACGAGAGACAACCAAGTTAGATCGAAAAACTTTAACTTTCTGTTGCATTCTGTCAAAGATAGACAAGAAATGAGCACATACAGACACAAGACAGGGTCACAGAGACACCATGCCATTTCATTTAAAGTGTCGTATTGATGCTTTATAGAAGTAGATAACTAAAATGTTTATATCAAATTCATGCATATTAAGTGTAAATTAATTAGTTTATTATATGAGCCAGGATGAGATCACGGAAGCAGTTGAACTTCGATTTTGAAGAACGAAAGCTGTGCGGAGCTCAAGTACGGGACTTGATAAAGAACCTTCCCAGACTTGTTAGCCACAACTCTTTATTTATACTTCTTATAGAGCCATGATGTGATGTGATGTGATGTTCTCATCTTAGGCACAAAATGAAATATTAAGCTAGAAAGTGCAGGCCAATAGTAAAGTCTACCTTCTCAGTTTTTAGTTGAACATTGCTGACAAGACTAACCTAATATGTAGGGATATATTATGAAATTTTCAATTTTTGTGTGCAACAGGAAAAGTAACTCAATCATAAATGGCATAGTCTACCTCCTTGTAGGTTCAAGATT

mRNA sequence

ATCCATGAAAATGCAAAATCCAACGATTTCCCGCCATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGAATGTTGTAGCCATAGCTTGGGGCTATGGAAACTACAACCCTTCATACGCAAACAAGAACGAAATGAAACCAATGTTTTGGTGACGAGAGACAACCAAGTTAGATCGAAAAACTTTAACTTTCTGTTGCATTCTGTCAAAGATAGACAAGAAATGAGCACATACAGACACAAGACAGGGTCACAGAGACACCATGCCATTTCATTTAAAGTGTCGTATTGATGCTTTATAGAAGTAGATAACTAAAATGTTTATATCAAATTCATGCATATTAAGTGTAAATTAATTAGTTTATTATATGAGCCAGGATGAGATCACGGAAGCAGTTGAACTTCGATTTTGAAGAACGAAAGCTGTGCGGAGCTCAAGTACGGGACTTGATAAAGAACCTTCCCAGACTTGTTAGCCACAACTCTTTATTTATACTTCTTATAGAGCCATGATGTGATGTGATGTGATGTTCTCATCTTAGGCACAAAATGAAATATTAAGCTAGAAAGTGCAGGCCAATAGTAAAGTCTACCTTCTCAGTTTTTAGTTGAACATTGCTGACAAGACTAACCTAATATGTAGGGATATATTATGAAATTTTCAATTTTTGTGTGCAACAGGAAAAGTAACTCAATCATAAATGGCATAGTCTACCTCCTTGTAGGTTCAAGATT

Coding sequence (CDS)

ATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGA

Protein sequence

MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMADCIRIAQLQQNCLGVHQVT
Homology
[Close]
BLAST of MELO3C020140.2 vs. NCBI nr
Match: XP_008456934.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >XP_008456936.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 889.8 bits (2298), Expect = 3.8e-255
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           ADCIRIAQLQQNCLGVHQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]
BLAST of MELO3C020140.2 vs. NCBI nr
Match: XP_011655038.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_5G220920 [Cucumis sativus])

HSP 1 Score: 850.5 bits (2196), Expect = 2.5e-243
Identity = 420/440 (95.45%), Postives = 428/440 (97.27%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

[Close]
BLAST of MELO3C020140.2 vs. NCBI nr
Match: XP_008456937.1 (PREDICTED: tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 808.1 bits (2086), Expect = 1.4e-230
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 400
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

[Close]
BLAST of MELO3C020140.2 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 797.0 bits (2057), Expect = 3.3e-227
Identity = 392/441 (88.89%), Postives = 416/441 (94.33%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIA-QLQQNCLGVHQVT 441
           A+CIRIA +LQQ+CLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]
BLAST of MELO3C020140.2 vs. NCBI nr
Match: XP_022986521.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima])

HSP 1 Score: 794.7 bits (2051), Expect = 1.6e-226
Identity = 391/441 (88.66%), Postives = 416/441 (94.33%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIA-QLQQNCLGVHQVT 441
           A+CIRIA +LQQ+CLGVHQVT
Sbjct: 421 AECIRIALELQQSCLGVHQVT 441

[Close]
BLAST of MELO3C020140.2 vs. TAIR10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 553.9 bits (1426), Expect = 8.9e-158
Identity = 270/430 (62.79%), Postives = 337/430 (78.37%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCL--SNNRTERGLDF 60
           +LT++  TLS S F  + ++ +    SP    F+   S K S +  L  S  + ER L+F
Sbjct: 2   LLTLSPKTLSSSSFFTVHKSQNPKCKSPDSCRFK---SLKCSFDRILVKSEVQIERNLEF 61

Query: 61  DIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLAN 120
           + G+TFFRHESA GRDLGVL+A LY++S G LR+LDA+CGCGIRSLRYLVE+EADFV+AN
Sbjct: 62  ETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMAN 121

Query: 121 DANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
           DAND+NRRVI  NLSKV    GD RRW+VT+  AN+ M E Y+  DFFD ID+DSFGSDS
Sbjct: 122 DANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDS 181

Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGV 240
           SFLR A NAL+L GLLY+TSTDG+SSGGHRP  SLAAYGA+IRPMPF NEIGLRMLIGG 
Sbjct: 182 SFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGA 241

Query: 241 AREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWS 300
            REA++LGY+ TPLFSYYSYHGPVFRVMLR++RG++ ++R+YGFV++C  CG+S    + 
Sbjct: 242 VREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFD 301

Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 360
           ELG M CPC+D++ S SLVVSGP+W+GPLH A Y+ ++L LAK+W W+  G G DL+KLL
Sbjct: 302 ELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLL 361

Query: 361 MQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNC 420
             M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT+C
Sbjct: 362 SIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDC 421

Query: 421 PMADCIRIAQ 429
           PM+  +RIA+
Sbjct: 422 PMSHFVRIAK 428

[Close]
BLAST of MELO3C020140.2 vs. TAIR10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 87.8 bits (216), Expect = 1.8e-17
Identity = 85/355 (23.94%), Postives = 148/355 (41.69%), Query Frame = 0 

Query: 91  RILDALCGCGIRSLRYLVESEA-DFVLANDANDENRRVIVKN--------LSKVAENFGD 150
           ++L+AL   G+R+LRY  E E    V+A D +  +     +N        +SKV  +  D
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHTD 182

Query: 151 NRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDG 210
            R  ++T+                FD +D+D +GS S FL  AI ++   GLL  T+TD 
Sbjct: 183 ARVHMLTH-------------PKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDM 242

Query: 211 FSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGP 270
               G       + YG+Y     + +E+ LR+L+  +   A+    Y  P+ S       
Sbjct: 243 AVLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF-- 302

Query: 271 VFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDS----------- 330
             RV +R+     +       +SY  +C    +F    +G+ S P N+S           
Sbjct: 303 YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVV 362

Query: 331 -----RVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDES 390
                   +   + GP+W  P+H  +++  IL   K  +        D    ++  + E 
Sbjct: 363 KQDCNHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK--DRYPAYDRISAVLTTVSEE 422

Query: 391 DSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
              +P+ ++ +  + +  K  SP  +   S +I A Y  S +H+    +KT+ PM
Sbjct: 423 LLDVPL-FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

[Close]
BLAST of MELO3C020140.2 vs. Swiss-Prot
Match: sp|B6YUU9|TRM1_THEON (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.6e-31
Identity = 114/373 (30.56%), Postives = 184/373 (49.33%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VLA     K+ G  R+LDAL   GIR +RY +E+ A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 124 NRRVIVKNL-----------SKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVD 183
             R+IV N+            K+A   G+ +  +VTN DANR+M E +    +FDF+D+D
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGE-KTLVVTNADANRLMNEQF---RYFDFVDLD 146

Query: 184 SFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYIRPMPFSNEIGL 243
            FGS   FL  A+ A+K  G+L +T+TD G   G +R +  L  Y A        +E GL
Sbjct: 147 PFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGL 206

Query: 244 RMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGN 303
           R+LIG + R A+        L +YY  H   FR  L++  G  + ++    + Y  +   
Sbjct: 207 RILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ--- 266

Query: 304 SQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQG 363
                  E G+      ++ +       GPLW+GPL + +++E++  L+ +   +G+   
Sbjct: 267 ------DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS--- 326

Query: 364 KDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIAS 423
           K  +K L  + DE +  +P  Y     VA R  +    L+ ++  + + GY A+R+H + 
Sbjct: 327 KKTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSP 370

Query: 424 NAIKTNCPMADCI 425
            A+KT+ P  + +
Sbjct: 387 TALKTDAPFEEVL 370

[Close]
BLAST of MELO3C020140.2 vs. Swiss-Prot
Match: sp|P81554|TRM1_PYRFU (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.7e-31
Identity = 108/369 (29.27%), Postives = 171/369 (46.34%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VL   + +       +LDAL   GIR +R+ +E+ A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVLLLNVLKPK----IVLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 124 NRRVIVKNL-----SKVAEN-----FGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN+       + EN     F   +  ++ N DANR+M E   K  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L VT+TDG    G  P   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 244 LIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKN---RHYGFVSYCEKCG 303
           L+G +AR A+        L +YY  H   FR  +++  G    +    + G+V + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 363
             +                S +S      GPLW+GPL +   + ++L L      + N  
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 364 GKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 420
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

[Close]
BLAST of MELO3C020140.2 vs. Swiss-Prot
Match: sp|Q9V1P3|TRM1_PYRAB (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 136.0 bits (341), Expect = 1.0e-30
Identity = 101/369 (27.37%), Postives = 178/369 (48.24%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ V+   + +       +LDAL   GIR +R+ +E+ A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVVLLNVLKPR----IVLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 124 NRRVIVKNL-----SKVAENFG-----DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN+      ++ E+ G       +  +V + DANR+M E   K  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L +T+TDG    G  P   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 244 LIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKN---RHYGFVSYCEKCG 303
           L+G VAR A+        + +YY  H   FR  +++  G    +    + G++ + E  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 363
                   E+ +   P   +         GP+W+GPL S + +E++L +++Q   +   +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 364 GKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 420
            K   KLL  + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

[Close]
BLAST of MELO3C020140.2 vs. Swiss-Prot
Match: sp|Q97ZH0|TRM1_SULSO (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=trm1 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.3e-30
Identity = 106/361 (29.36%), Postives = 188/361 (52.08%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVES-EADFVLANDAND 123
           F+  +    RDL V+  ++ +       I+DAL   GIR +RY VES +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPK----IIVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 124 ENRRVIVKNLSKVAENFG-DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFL 183
               +I  N    A+N G +N +  + N DAN ++ E        D+ID+D FGS + F+
Sbjct: 95  NATSLIQIN----AKNNGIENAK--IYNKDANALLYEVK-----SDYIDIDPFGSPAPFI 154

Query: 184 RPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVARE 243
             ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + RE
Sbjct: 155 LSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIRE 214

Query: 244 ASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISK----NRHYGFVSYCEKCGNSQAFSW 303
           A++L     PLFS+Y+ +   +R+ +R+  G        N+H  +   C +CG  Q F  
Sbjct: 215 AAILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG-FQTFV- 274

Query: 304 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKL 363
            E  +  CP       E   + GPL+IGPL+S ++++ I+ L   + ++ +     ++KL
Sbjct: 275 EENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKL 334

Query: 364 LMQMLDESDSKLPVGYIKMDEVASRAKVNS-PPLSTMMSEMIKAGYAASRSHIASNAIKT 418
           L  +  E +++    +  + ++AS+ K+++ PP+ +++  +      ASR+H A   I+T
Sbjct: 335 LNVI--EKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRT 364

[Close]
BLAST of MELO3C020140.2 vs. Swiss-Prot
Match: sp|C5A6N2|TRM1_THEGJ (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) OX=593117 GN=trm1 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.7e-29
Identity = 114/379 (30.08%), Postives = 177/379 (46.70%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VLA     K+    R+LDAL   GIR LRY +E+ A+ V  ND N++
Sbjct: 27  FYNPVMALNRDISVLAV----KALSPRRVLDALSATGIRGLRYSLETSAEEVWMNDINED 86

Query: 124 NRRVIVKNLSKVAENFGD--------------NRRWLVTNCDANRVMTEFYLKKDFFDFI 183
             R+I+KNL     NFG                +R +V   DAN++M+E +    +FDFI
Sbjct: 87  AFRLILKNLEL---NFGSKPVLANGRAELGLPEKRLVVNLGDANKLMSENF---RYFDFI 146

Query: 184 DVDSFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYIRPMPFSNE 243
           D+D FGS   FL  A+ +++  G L +T+TD G   G +R +         IR     +E
Sbjct: 147 DLDPFGSPVEFLDSALRSVRRRGFLGLTATDTGVLCGAYRRACRRKYLAEPIRG-ELCHE 206

Query: 244 IGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEK 303
            GLR+LIG + R A+        L +YY  H   FRV LR+  G    +     + Y   
Sbjct: 207 AGLRILIGTIVRYAAKYDLGVEVLLAYYRDH--YFRVFLRLRSGAKKADESVELLGYL-- 266

Query: 304 CGNSQAFSW-SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAK-QWEWI 363
                   W  E G+         +       GP+W+GPL +  ++E+++ L K   E I
Sbjct: 267 --------WQDETGKFK--YERDFLPGKRGAYGPMWLGPLKNQAFLEELVRLTKTSEESI 326

Query: 364 GNGQGKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASR 423
            + +      LL++ LD     +P  Y     +A +  +    +S ++  +   G+ A+R
Sbjct: 327 SHRKTFQFLSLLVEELD-----VPFHY-DTHALARKNGLEVVKISRVIETLHSLGFEATR 374

Query: 424 SHIASNAIKTNCPMADCIR 426
           +H +  +IKTN P    +R
Sbjct: 387 THFSPTSIKTNAPFDVVLR 374

[Close]
BLAST of MELO3C020140.2 vs. TrEMBL
Match: tr|A0A1S3C509|A0A1S3C509_CUCME (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 2.5e-255
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           ADCIRIAQLQQNCLGVHQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]
BLAST of MELO3C020140.2 vs. TrEMBL
Match: tr|A0A0A0KP99|A0A0A0KP99_CUCSA (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 1.7e-243
Identity = 420/440 (95.45%), Postives = 428/440 (97.27%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

[Close]
BLAST of MELO3C020140.2 vs. TrEMBL
Match: tr|A0A1S3C4F5|A0A1S3C4F5_CUCME (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 9.5e-231
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 400
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

[Close]
BLAST of MELO3C020140.2 vs. TrEMBL
Match: tr|M5XJK8|M5XJK8_PRUPE (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Prunus persica OX=3760 GN=PRUPE_2G262600 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.3e-171
Identity = 303/435 (69.66%), Postives = 353/435 (81.15%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSP--NNCLSNNRTERGLDF 60
           M+T+   TLSP  FL           S    + Q+S SN K    +NC S  +TERGL F
Sbjct: 1   MITLTPKTLSPFSFLCNPIPKHQNQNSKYTIVTQYSDSNSKPTFNSNCESEFQTERGLKF 60

Query: 61  DIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLAN 120
           DIG+TFFRHESATGRDLGVLAAALY++SKG LR+LDALCGCGIRSLRYL+E+EADFVLAN
Sbjct: 61  DIGETFFRHESATGRDLGVLAAALYKRSKGKLRVLDALCGCGIRSLRYLIEAEADFVLAN 120

Query: 121 DANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
           DAND+NR+VI +NLS+V    GD+ RW VT  DANR+MTE YLK+DFFD ID+DSFGSDS
Sbjct: 121 DANDDNRKVITENLSQVES--GDDPRWAVTLSDANRIMTECYLKRDFFDLIDIDSFGSDS 180

Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGV 240
           SFLR AINALKLDGLLYVTSTDGFSSGG RP Q+LAAYGAY+RPMP+SNE+GLRMLIGG 
Sbjct: 181 SFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPMPYSNELGLRMLIGGA 240

Query: 241 AREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWS 300
            R ASVLGY  TPLFSYYSYHGP+FRVMLR+NRG++  NR+YGF+SYC  CG+SQ +SW+
Sbjct: 241 VRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFISYCHNCGSSQEYSWA 300

Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 360
           ELG+MSCPC+DS+VS+SL+VSGPLW GPLH   YI ++L LA++W W G+G G DL+KLL
Sbjct: 301 ELGRMSCPCSDSKVSKSLIVSGPLWTGPLHDTIYITEMLHLAEEWGWAGSGSGTDLDKLL 360

Query: 361 MQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNC 420
            QM+ ESD +LP GY K+DEVASRAKVNSPPL  +MS + K GYAASRSHI SNAIKTNC
Sbjct: 361 NQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYAASRSHIESNAIKTNC 420

Query: 421 PMADCIRIAQLQQNC 434
            MA CIRIA+  Q C
Sbjct: 421 SMAGCIRIAKQLQQC 433

[Close]
BLAST of MELO3C020140.2 vs. TrEMBL
Match: tr|A0A2N9J6K0|A0A2N9J6K0_FAGSY (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60350 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 8.5e-171
Identity = 305/436 (69.95%), Postives = 358/436 (82.11%), Query Frame = 0 

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           ML +   TLSPSPFL+      + ++   I   QF  +      NC S+  TERGL+F  
Sbjct: 1   MLNLTPKTLSPSPFLF------NPISQNPIFTVQFKPTTNA---NCKSDYHTERGLEFQA 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           G+TFFRHESATGRDLGVLAA L++KSK  LR+LDALCGCGIRSLRYLVE+EADFVLAND 
Sbjct: 61  GETFFRHESATGRDLGVLAAYLHKKSKSKLRVLDALCGCGIRSLRYLVEAEADFVLANDG 120

Query: 121 NDENRRVIVKNL--SKVAENFGDN-RRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSD 180
           NDE RR+I +NL  S+V   FGD+ RRW VT+ DANR+MTE YL++D FD IDVDSFGSD
Sbjct: 121 NDEYRRIISENLCRSRVPRGFGDDERRWAVTHFDANRLMTECYLQRDLFDLIDVDSFGSD 180

Query: 181 SSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGG 240
           SSFLR A+NALKLDGLLY+TSTDG++SGGHRPS SLAAYGAY+RPMP+SNE+GLRMLIGG
Sbjct: 181 SSFLRSAMNALKLDGLLYITSTDGYTSGGHRPSHSLAAYGAYVRPMPYSNEVGLRMLIGG 240

Query: 241 VAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSW 300
             +EASVLGY  TPLFSYYSYHGPVFRVMLR+NRG++  +R+YGF+SYC +CGNS +FSW
Sbjct: 241 AVKEASVLGYSVTPLFSYYSYHGPVFRVMLRLNRGKLVDDRYYGFISYCHQCGNSHSFSW 300

Query: 301 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKL 360
            +LGQ+SCPC+DS+VS SLVVSGPLW GPLH A Y+ ++L LA+Q  W+G+G+G  LEKL
Sbjct: 301 DQLGQISCPCSDSKVSSSLVVSGPLWTGPLHDAAYVTEMLNLAEQRGWVGSGKGTHLEKL 360

Query: 361 LMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTN 420
           L QM+DESD  LP+GYIKMDEVASRAK+NSPPL TMMS M K GYAASRSHIASNAIKTN
Sbjct: 361 LKQMVDESDPNLPIGYIKMDEVASRAKINSPPLMTMMSTMHKEGYAASRSHIASNAIKTN 420

Query: 421 CPMADCIRIAQ-LQQN 433
           CPM  CIRIA+ LQQ+
Sbjct: 421 CPMTVCIRIAKNLQQS 427

The following BLAST results are available for this feature:
BLAST of MELO3C020140.2 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008456934.13.8e-255100.00PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo]... [more]
XP_011655038.12.5e-24395.45PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011... [more]
XP_008456937.11.4e-230100.00PREDICTED: tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase isoform ... [more]
XP_022943113.13.3e-22788.89tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata][more]
XP_022986521.11.6e-22688.66tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima][more]
BLAST of MELO3C020140.2 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G56330.18.9e-15862.79N2,N2-dimethylguanosine tRNA methyltransferase[more]
AT3G02320.11.8e-1723.94N2,N2-dimethylguanosine tRNA methyltransferase[more]
BLAST of MELO3C020140.2 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|B6YUU9|TRM1_THEON1.6e-3130.56tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N... [more]
sp|P81554|TRM1_PYRFU2.7e-3129.27tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ... [more]
sp|Q9V1P3|TRM1_PYRAB1.0e-3027.37tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O... [more]
sp|Q97ZH0|TRM1_SULSO2.3e-3029.36tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus solfataricus (strain A... [more]
sp|C5A6N2|TRM1_THEGJ5.7e-2930.08tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strai... [more]
BLAST of MELO3C020140.2 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3C509|A0A1S3C509_CUCME2.5e-255100.00tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496... [more]
tr|A0A0A0KP99|A0A0A0KP99_CUCSA1.7e-24395.45tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
tr|A0A1S3C4F5|A0A1S3C4F5_CUCME9.5e-231100.00tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496... [more]
tr|M5XJK8|M5XJK8_PRUPE1.3e-17169.66tRNA (guanine(26)-N(2))-dimethyltransferase OS=Prunus persica OX=3760 GN=PRUPE_2... [more]
tr|A0A2N9J6K0|A0A2N9J6K0_FAGSY8.5e-17169.95tRNA (guanine(26)-N(2))-dimethyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_L... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003723RNA binding
GO:0004809tRNA (guanine-N2-)-methyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0008033tRNA processing
Vocabulary: INTERPRO
TermDefinition
IPR029063SAM-dependent_MTases
IPR002905Trm1
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0030488 tRNA methylation
biological_process GO:0008033 tRNA processing
biological_process GO:0016556 mRNA modification
biological_process GO:0002940 tRNA N2-guanine methylation
biological_process GO:0009451 RNA modification
cellular_component GO:0005575 cellular_component
cellular_component GO:0009536 plastid
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0016740 transferase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C020140.2.1MELO3C020140.2.1mRNA


InterPro
Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.56.70coord: 372..428
e-value: 2.8E-11
score: 45.0
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 34..367
e-value: 8.7E-73
score: 246.8
NoneNo IPR availablePANTHERPTHR10631:SF9N2, N2-DIMETHYLGUANOSINE TRNA METHYLTRANSFERASES-LIKE PROTEINcoord: 36..431
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 91..197
e-value: 8.05528E-5
score: 39.7207
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 64..426
e-value: 2.7E-65
score: 220.9
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 36..431
IPR002905tRNA methyltransferase, Trm1PROSITEPS51626SAM_MT_TRM1coord: 49..427
score: 92.743
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILYSSF53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 52..427