Cp4.1LG01g11850 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG01g11850 vs. Swiss-Prot
Match: TRM1_THEON (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) GN=trm1 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 9.4e-35
Identity = 122/379 (32.19%), Postives = 184/379 (48.55%), Query Frame = 1

Query: 30  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 89
           F+    A  RD+ VLA     K+ GS RVLDAL   GIR +RY +E  A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 90  NRGVIVKNLS-----------KVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVD 149
              +IV N+            K+A   GE +  +VTN DANR+M+E   Q  +FDF+D+D
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGE-KTLVVTNADANRLMNE---QFRYFDFVDLD 146

Query: 150 SFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLR 209
            FGS   FL  A+ A+K  G+L +T+TD     G + +  L  Y A        +E GLR
Sbjct: 147 PFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYRNACLRKYLAEPIRGELCHEAGLR 206

Query: 210 MLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNS 269
           +LIG + R A+     V  L +YY  H   FR  L++  G    ++    + Y  +    
Sbjct: 207 ILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ---- 266

Query: 270 QAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGK 329
                 E G+      +T +       GPLW GPL + +++E++  L+ +   +G+   K
Sbjct: 267 -----DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS---K 326

Query: 330 DLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASN 389
             +K LG + DE +  +P  Y     VA R  +    L  ++  + + GY A+R+H +  
Sbjct: 327 KTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPT 377

Query: 390 AIKTNCPMTECIRIALELQ 398
           A+KT+ P  E +    ELQ
Sbjct: 387 ALKTDAPFEEVLEALRELQ 377

BLAST of Cp4.1LG01g11850 vs. Swiss-Prot
Match: TRM1_PYRAB (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=trm1 PE=3 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 1.3e-31
Identity = 103/369 (27.91%), Postives = 176/369 (47.70%), Query Frame = 1

Query: 30  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 89
           F+    A  RD+ V+   + +       VLDAL   GIR +R+ +E  A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVVLLNVLKPRI----VLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 90  NRGVIVKNL-----SKVAEGFGET-----RRWMVTNCDANRVMSEFYLQKDFFDFIDVDS 149
              ++ KN+      ++ E  G       +  +V + DANR+M+E   +  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 150 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 209
           FGS   FL  A+ ++K  G+L +T+TDG    G HP   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 210 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNR---HYGFVSYCKKCG 269
           L+G VAR A+     +  + +YY  H   FR  +++  G    +    + G++ + +  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 270 NSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQ 329
                   EV +   P             GP+W GPL S + +E+ML ++ Q   +   +
Sbjct: 267 KF------EVERSFLPSKPNAY-------GPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 330 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 386
            K   KLL  + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

BLAST of Cp4.1LG01g11850 vs. Swiss-Prot
Match: TRM1_PYRFU (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) GN=trm1 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.7e-31
Identity = 109/369 (29.54%), Postives = 173/369 (46.88%), Query Frame = 1

Query: 30  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 89
           F+    A  RD+ VL   + +       VLDAL   GIR +R+ +E  A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVLLLNVLKPKI----VLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 90  NRGVIVKNLSKVAEG----------FGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDS 149
              ++ KN+    +G          F   +  ++ N DANR+M+E   +  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 150 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 209
           FGS   FL  A+ ++K  G+L VT+TDG    G HP   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 210 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKN---RHYGFVSYCKKCG 269
           L+G +AR A+     +  L +YY  H   FR  +++  G    +    + G+V + +K G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 270 NSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQ 329
             +     E   +S   N           GPLW GPL +   + +ML L +    + N  
Sbjct: 269 RFEV----EKSFLSTRPN---------AYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 330 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 386
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

BLAST of Cp4.1LG01g11850 vs. Swiss-Prot
Match: TRM1_SULSO (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=trm1 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.4e-30
Identity = 105/371 (28.30%), Postives = 190/371 (51.21%), Query Frame = 1

Query: 30  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEA-EADFVLANDAND 89
           F+  +    RDL V+  ++ +       ++DAL   GIR +RY VE+ +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPKI----IVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 90  ENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLR 149
               +I  N      G    +   + N DAN ++ E        D+ID+D FGS + F+ 
Sbjct: 95  NATSLIQINAKN--NGIENAK---IYNKDANALLYEVKS-----DYIDIDPFGSPAPFIL 154

Query: 150 PAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREA 209
            ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + REA
Sbjct: 155 SSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREA 214

Query: 210 SVLGFYVTPLFSYYSYHGPVFRVMLRISRGR----INKNRHYGFVSYCKKCGNSQAFSWS 269
           ++L   V PLFS+Y+ +   +R+ +R+  G      N N+H  +   C +CG  Q F   
Sbjct: 215 AILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG-FQTFV-E 274

Query: 270 EVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLL 329
           E  +  CP       E   + GPL+ GPL+S ++++ ++ L   + ++ +     ++KLL
Sbjct: 275 ENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKLL 334

Query: 330 GQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLNTMMSEMIKAGYAASRSHIASNAIKTN 389
              V E +++    +  + ++AS+ K+++ PP+ +++  +      ASR+H A   I+T+
Sbjct: 335 N--VIEKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRTD 375

Query: 390 C---PMTECIR 392
                +T+CI+
Sbjct: 395 KEYEEITKCIK 375

BLAST of Cp4.1LG01g11850 vs. Swiss-Prot
Match: TRM1_SULIY (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1) GN=trm1 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 9.1e-30
Identity = 109/374 (29.14%), Postives = 189/374 (50.53%), Query Frame = 1

Query: 30  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEA-EADFVLANDAND 89
           F+  +    RDL V+  +L +       +LDAL   GIR +RY VE+ +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSLLKPKI----ILDALSATGIRGIRYYVESWKSEQLILNDKNS 94

Query: 90  ENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLR 149
               +I  N+     G    +   + N DAN ++ E   +     +ID+D FGS   F+ 
Sbjct: 95  TAASLIQINVKN--NGIENAK---IYNKDANALLYEIKSE-----YIDIDPFGSPVPFIL 154

Query: 150 PAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREA 209
            ++NA   +G++  T+TD     G   +     Y A    +  S E+GLR+LIG + REA
Sbjct: 155 SSVNATIRNGIVAFTATDLSPLEGSSRTSCRRKYDAINYKLSSSKELGLRILIGKIIREA 214

Query: 210 SVLGFYVTPLFSYYS-YHGPVFRVM---LRISRGRINKNRHYGFVSYCKKCGNSQAFSWS 269
           + L   V PLFS+Y+ Y+  +F ++    R +   INKN  Y     C +CG  Q F   
Sbjct: 215 ATLEKTVHPLFSFYADYYYRLFAIVESGARKADENINKNLKY--FGECPRCG-FQTFV-D 274

Query: 270 EVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLL 329
           E  +  CP       E+ ++ GPL+ GPLH+ ++++ M+     + ++ +     ++KLL
Sbjct: 275 ENCKTKCPI----CGENFIIIGPLYIGPLHNMEFLKRMIDTYSDFNYLSS--FNRIQKLL 334

Query: 330 GQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLNTMMSEMIKAGYAASRSHIASNAIKTN 389
              V E ++K    +  + ++AS+ KV++ PP+++++  +      AS++H A   I+T+
Sbjct: 335 N--VIEKEAKYKSVFYNISKLASKLKVSAIPPIDSILECL----GDASKTHFAPTGIRTD 378

Query: 390 CPMTECIRIALELQ 398
               E IR    L+
Sbjct: 395 KGYEEIIRCVKSLR 378

BLAST of Cp4.1LG01g11850 vs. TrEMBL
Match: A0A0A0KP99_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G220920 PE=4 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 2.3e-213
Identity = 365/398 (91.71%), Postives = 384/398 (96.48%), Query Frame = 1

Query: 9   LNNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIR 68
           LNNCL NNRTERG++FDIGDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIR
Sbjct: 43  LNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIR 102

Query: 69  SLRYLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQ 128
           SLRYLVE+EADFVLANDANDENR VIVKNLS+VAE  G+ RRW+VTNCDANRVM+EFYL+
Sbjct: 103 SLRYLVESEADFVLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLK 162

Query: 129 KDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRP 188
           KDFFDFIDVDSFGSDS+FLRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRP
Sbjct: 163 KDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRP 222

Query: 189 MPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGF 248
           MPFSNEIGLRMLIGGVAREASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGF
Sbjct: 223 MPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGF 282

Query: 249 VSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQ 308
           VSYC+KCGNSQAFSWSE+GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA Q
Sbjct: 283 VSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQ 342

Query: 309 WEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGY 368
           WEWIGNGQGKDLEKLL QMVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGY
Sbjct: 343 WEWIGNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGY 402

Query: 369 AASRSHIASNAIKTNCPMTECIRIALELQQSCLGVHQV 407
           AASRSHIASNAIKTNCPM ECIRIA +LQ++CLG HQV
Sbjct: 403 AASRSHIASNAIKTNCPMAECIRIA-QLQRNCLGAHQV 439

BLAST of Cp4.1LG01g11850 vs. TrEMBL
Match: B9I878_POPTR (N2 family protein OS=Populus trichocarpa GN=POPTR_0013s09180g PE=4 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 2.9e-168
Identity = 286/387 (73.90%), Postives = 331/387 (85.53%), Query Frame = 1

Query: 12  CLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLR 71
           C    ++ERG+EF+ G+TFFRHESA GRDLGVLAA+LY +SKG LRVLDALCGCGIRSLR
Sbjct: 35  CKSELQSERGLEFETGETFFRHESAKGRDLGVLAASLYNQSKGKLRVLDALCGCGIRSLR 94

Query: 72  YLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDF 131
           YL+EA+ADFVLANDANDE+R VI++NL +V  GFG+ R+W+VT+ DANRV+SE YLQKDF
Sbjct: 95  YLMEAKADFVLANDANDEHRRVILENLKRVDRGFGDERKWVVTHLDANRVLSECYLQKDF 154

Query: 132 FDFIDVDSFGSD-STFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMP 191
           FD ID+DSFGSD S FLR A+N L  DGLLYVTSTDG SSGGHHP  SLAAYGAYVRPMP
Sbjct: 155 FDLIDIDSFGSDISLFLRSAMNTLSFDGLLYVTSTDGHSSGGHHPDHSLAAYGAYVRPMP 214

Query: 192 FSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVS 251
           +SNE+GLRMLIGG  REASVLG+Y+TPLFSYYSYHGPVFRVMLR++RG++ +NRHYGF++
Sbjct: 215 YSNEVGLRMLIGGAVREASVLGYYITPLFSYYSYHGPVFRVMLRVNRGKLLENRHYGFIT 274

Query: 252 YCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWE 311
           YC  CGNSQ FSW+E+GQ+SC C+ +  S SLV+SGPLWTGPLHSA YI  M+ LA +W 
Sbjct: 275 YCNNCGNSQEFSWAELGQISCSCSGSHGSRSLVISGPLWTGPLHSAAYISKMINLAEEWG 334

Query: 312 WIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAA 371
           WIGNG   DLEKLL QM+DESD +L  GYIKMDEVASRAK+NSPPL TMMS M K GYA 
Sbjct: 335 WIGNGAETDLEKLLKQMLDESDPRLKSGYIKMDEVASRAKINSPPLRTMMSAMQKEGYAT 394

Query: 372 SRSHIASNAIKTNCPMTECIRIALELQ 398
           SRSHIASNAIKT+CPM+ CIRI+ ELQ
Sbjct: 395 SRSHIASNAIKTDCPMSVCIRISKELQ 421

BLAST of Cp4.1LG01g11850 vs. TrEMBL
Match: M5XJK8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005915mg PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 9.4e-167
Identity = 285/389 (73.26%), Postives = 333/389 (85.60%), Query Frame = 1

Query: 10  NNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRS 69
           +NC    +TERG++FDIG+TFFRHESATGRDLGVLAAALY++SKG LRVLDALCGCGIRS
Sbjct: 46  SNCESEFQTERGLKFDIGETFFRHESATGRDLGVLAAALYKRSKGKLRVLDALCGCGIRS 105

Query: 70  LRYLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQK 129
           LRYL+EAEADFVLANDAND+NR VI +NLS+V  G  +  RW VT  DANR+M+E YL++
Sbjct: 106 LRYLIEAEADFVLANDANDDNRKVITENLSQVESG--DDPRWAVTLSDANRIMTECYLKR 165

Query: 130 DFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPM 189
           DFFD ID+DSFGSDS+FLR AINALKLDGLLYVTSTDGFSSGG  P Q+LAAYGAYVRPM
Sbjct: 166 DFFDLIDIDSFGSDSSFLRSAINALKLDGLLYVTSTDGFSSGGRRPQQTLAAYGAYVRPM 225

Query: 190 PFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFV 249
           P+SNE+GLRMLIGG  R ASVLG+ VTPLFSYYSYHGP+FRVMLR++RG++  NR+YGF+
Sbjct: 226 PYSNELGLRMLIGGAVRVASVLGYRVTPLFSYYSYHGPIFRVMLRVNRGKLPDNRNYGFI 285

Query: 250 SYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQW 309
           SYC  CG+SQ +SW+E+G+MSCPC+D++VS+SL+VSGPLWTGPLH   YI +ML LA +W
Sbjct: 286 SYCHNCGSSQEYSWAELGRMSCPCSDSKVSKSLIVSGPLWTGPLHDTIYITEMLHLAEEW 345

Query: 310 EWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYA 369
            W G+G G DL+KLL QMV ESD +LP GY K+DEVASRAKVNSPPL  +MS + K GYA
Sbjct: 346 GWAGSGSGTDLDKLLNQMVAESDPRLPFGYTKLDEVASRAKVNSPPLRAVMSTLEKEGYA 405

Query: 370 ASRSHIASNAIKTNCPMTECIRIALELQQ 399
           ASRSHI SNAIKTNC M  CIRIA +LQQ
Sbjct: 406 ASRSHIESNAIKTNCSMAGCIRIAKQLQQ 432

BLAST of Cp4.1LG01g11850 vs. TrEMBL
Match: A5ACL3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g02690 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.2e-166
Identity = 286/393 (72.77%), Postives = 335/393 (85.24%), Query Frame = 1

Query: 8   SLNNCLPNN--RTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGC 67
           S +  LP +  RTERG+ FD G++FFRHESATGRDLGVLAA+LY+KSKGS+RVLDA+CGC
Sbjct: 31  SASQALPKSEYRTERGLTFDSGNSFFRHESATGRDLGVLAASLYKKSKGSVRVLDAMCGC 90

Query: 68  GIRSLRYLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEF 127
           GIRSLRYLVEAEADFVLANDAN+     I++NLS+   G GE RRW+VT+ DANRVM+E 
Sbjct: 91  GIRSLRYLVEAEADFVLANDANENYGRTILENLSQGLGGSGEERRWVVTHTDANRVMTEC 150

Query: 128 YLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAY 187
           YL++DF+D IDVDSFGS+S+FLR A NALKLDGL YVTSTDG++SGGH P  SLA+YGA+
Sbjct: 151 YLRRDFYDLIDVDSFGSESSFLRSAYNALKLDGLFYVTSTDGYTSGGHRPHHSLASYGAF 210

Query: 188 VRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRH 247
           VRPMP+SNE+GLRMLIGG  REASVLG+ +TPLFSYYSYHGPVFRVMLR++RG++ +NR+
Sbjct: 211 VRPMPYSNEVGLRMLIGGALREASVLGYRITPLFSYYSYHGPVFRVMLRMNRGKLPENRN 270

Query: 248 YGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTL 307
           YGF+SYC KCGNSQAFSW+E+G++SCPC+D +VS SLVVSGPLW GPLH+A Y+ +ML L
Sbjct: 271 YGFISYCTKCGNSQAFSWNELGRISCPCSDAKVSSSLVVSGPLWIGPLHNASYLTEMLNL 330

Query: 308 AMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIK 367
           A QW W GN     L KLL QM+DESD KLP GYIK+DEVASRAK+NSPPL TMMS + K
Sbjct: 331 AEQWGWTGNDTETHLGKLLKQMIDESDPKLPFGYIKLDEVASRAKINSPPLRTMMSALNK 390

Query: 368 AGYAASRSHIASNAIKTNCPMTECIRIALELQQ 399
            GYA SRSHIASNAIKTNCPM  CIRIA ELQQ
Sbjct: 391 EGYAVSRSHIASNAIKTNCPMAVCIRIAKELQQ 423

BLAST of Cp4.1LG01g11850 vs. TrEMBL
Match: A0A067JFV0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_03334 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 9.7e-164
Identity = 275/387 (71.06%), Postives = 330/387 (85.27%), Query Frame = 1

Query: 11  NCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSL 70
           NC    +TERGI F+ G TFF+HESA GRDLGVLAA+LY++SKG+LRVLDA+CGCGIRS 
Sbjct: 36  NCKFELQTERGIVFETGGTFFQHESAKGRDLGVLAASLYKQSKGNLRVLDAMCGCGIRSC 95

Query: 71  RYLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKD 130
           RYL+EA+ADFVLANDANDE RG+I+ NL KV  GFG+ RRW V++ DANRV++E YLQ+D
Sbjct: 96  RYLIEAKADFVLANDANDEYRGIILGNLKKVERGFGDDRRWAVSHFDANRVLTECYLQRD 155

Query: 131 FFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMP 190
           FFD ID+DSFGSDS FLR A++ALKLDGLLY+TSTDG+SSGGH P  SLAAYGAY+RPMP
Sbjct: 156 FFDLIDIDSFGSDSLFLRSAMSALKLDGLLYLTSTDGYSSGGHRPHCSLAAYGAYIRPMP 215

Query: 191 FSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVS 250
             NEIGLRMLIGG  REAS+LG++VTPLFSYYSYHGPVFRVML++++G+ ++NRHYGF+S
Sbjct: 216 SCNEIGLRMLIGGAVREASLLGYHVTPLFSYYSYHGPVFRVMLQVNKGKDHENRHYGFIS 275

Query: 251 YCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWE 310
           YC +CGN+Q+FSW E+GQ SC CN +  S  + VSGPLWTGPLH+A +I +ML LA QW 
Sbjct: 276 YCNQCGNTQSFSWEELGQSSCSCNSSNDSSPVTVSGPLWTGPLHNATFITEMLNLAEQWG 335

Query: 311 WIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAA 370
           WIGN  G DL+KLL +M+DESD +LP GYIKMDE+ASR K+NSP L TMMS ++K GYAA
Sbjct: 336 WIGNDVGADLDKLLKRMLDESDPRLPFGYIKMDEMASRGKINSPSLKTMMSTLVKKGYAA 395

Query: 371 SRSHIASNAIKTNCPMTECIRIALELQ 398
           +RSHIASNAIKT+CPM+ CIRIA ELQ
Sbjct: 396 TRSHIASNAIKTDCPMSMCIRIAKELQ 422

BLAST of Cp4.1LG01g11850 vs. TAIR10
Match: AT3G56330.1 (AT3G56330.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 548.5 bits (1412), Expect = 3.9e-156
Identity = 255/377 (67.64%), Postives = 311/377 (82.49%), Query Frame = 1

Query: 19  ERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEA 78
           ER +EF+ G+TFFRHESA GRDLGVL+A LY++S GSLRVLDA+CGCGIRSLRYLVEAEA
Sbjct: 53  ERNLEFETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEA 112

Query: 79  DFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVD 138
           DFV+ANDAND+NR VI  NLSKV  G G+ RRW+VT+  AN+ M E Y+  DFFD ID+D
Sbjct: 113 DFVMANDANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDID 172

Query: 139 SFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLR 198
           SFGSDS+FLR A NAL+L GLLY+TSTDG+SSGGH P  SLAAYGA++RPMPF NEIGLR
Sbjct: 173 SFGSDSSFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLR 232

Query: 199 MLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNS 258
           MLIGG  REA++LG++VTPLFSYYSYHGPVFRVMLR+ RG+++++R+YGFV++C  CG+S
Sbjct: 233 MLIGGAVREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHS 292

Query: 259 QAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGK 318
               + E+G M CPC+DT+ S SLVVSGP+W GPLH A Y+ +ML LA +W W+  G G 
Sbjct: 293 HTLRFDELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGM 352

Query: 319 DLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASN 378
           DL+KLL  M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  N
Sbjct: 353 DLDKLLSIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPN 412

Query: 379 AIKTNCPMTECIRIALE 396
           A+KT+CPM+  +RIA E
Sbjct: 413 ALKTDCPMSHFVRIAKE 429

BLAST of Cp4.1LG01g11850 vs. TAIR10
Match: AT5G15810.1 (AT5G15810.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 94.7 bits (234), Expect = 1.5e-19
Identity = 100/415 (24.10%), Postives = 169/415 (40.72%), Query Frame = 1

Query: 25  DIGDTFFRHESATGRDLGVLAAALYRKSKGSL---RVLDALCGCGIRSLRYLVEAEA-DF 84
           D G T   HE  T       A   Y  ++  L   RVL+AL   G+R+LRY  E E    
Sbjct: 186 DNGKTNGEHEVTTQDGPKEAAKTAYESARRELKPPRVLEALSASGLRALRYAREVEGIGQ 245

Query: 85  VLANDANDENRGVIVKNL--------SKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFF 144
           V+A D +  +     +N+        SKV     + R  M+++             KD F
Sbjct: 246 VVALDNDPASVEACQRNIKFNGLMSTSKVESHLTDARVHMLSH------------PKD-F 305

Query: 145 DFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFS 204
           D +D+D +G+ S FL  A+ ++   GLL  T+TD     G +     + YG+Y     + 
Sbjct: 306 DVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVLCGANGEVCYSKYGSYPLKGKYC 365

Query: 205 NEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYC 264
           +E+ LR+L+  +   A+    Y+ P+ S         RV +R+             +SY 
Sbjct: 366 HEMALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVRVYTSASAMKNTPLKLSYV 425

Query: 265 KKCGNSQAFSWSEVGQMSCPCND----------------TRVSESLVVSGPLWTGPLHSA 324
            +C    +F    VG+ S P N+                T   +   + GP+W+ P+H  
Sbjct: 426 YQCIGCDSFHLQSVGR-SLPKNNSVRYLPGVGPVVPQDCTHCGKKYNMGGPIWSAPIHDQ 485

Query: 325 DYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPL 384
           +++  +L      +        D    +   + E    +P+ ++ +  +++  K  SP  
Sbjct: 486 EWVNSILNGVKSMK--DRYPAYDRICAVLTTISEELPDVPL-FLSLHSLSATLKCTSPSA 545

Query: 385 NTMMSEMIKAGYAASRSHIASNAIKTNCPMTECIRIALELQQSCLGVHQVMPPLP 412
               S +I A Y  S SH+    IKT+ PM     I  ++ +  +  H + P  P
Sbjct: 546 ALFRSAVINAKYRVSGSHVNPLGIKTDAPM----EIIWDIMRCWVKNHPIKPQSP 577

BLAST of Cp4.1LG01g11850 vs. TAIR10
Match: AT3G02320.1 (AT3G02320.1 N2,N2-dimethylguanosine tRNA methyltransferase)

HSP 1 Score: 85.1 bits (209), Expect = 1.2e-16
Identity = 84/356 (23.60%), Postives = 148/356 (41.57%), Query Frame = 1

Query: 57  RVLDALCGCGIRSLRYLVEAEA-DFVLANDANDENRGVIVKNL--------SKVAEGFGE 116
           +VL+AL   G+R+LRY  E E    V+A D +  +     +N+        SKV     +
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHTD 182

Query: 117 TRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDG 176
            R  M+T+                FD +D+D +GS S FL  AI ++   GLL  T+TD 
Sbjct: 183 ARVHMLTHPKE-------------FDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDM 242

Query: 177 FSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGP 236
               G +     + YG+Y     + +E+ LR+L+  +   A+    Y+ P+ S       
Sbjct: 243 AVLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF-- 302

Query: 237 VFRVMLRISRGRINKNRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDT----------- 296
             RV +R+             +SY  +C    +F    VG+ S P N++           
Sbjct: 303 YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVV 362

Query: 297 -----RVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 356
                   +   + GP+W+ P+H  +++  +L      +     +    +++   +   S
Sbjct: 363 KQDCNHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK----DRYPAYDRISAVLTTVS 422

Query: 357 DSKLPVG-YIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 387
           +  L V  ++ +  + +  K  SP      S +I A Y  S +H+    +KT+ PM
Sbjct: 423 EELLDVPLFLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

BLAST of Cp4.1LG01g11850 vs. NCBI nr
Match: gi|659114163|ref|XP_008456934.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 753.1 bits (1943), Expect = 3.0e-214
Identity = 366/403 (90.82%), Postives = 388/403 (96.28%), Query Frame = 1

Query: 4   SQIKSLNNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALC 63
           S  KS NNCL NNRTERG++FDIGDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALC
Sbjct: 38  SNKKSPNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALC 97

Query: 64  GCGIRSLRYLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMS 123
           GCGIRSLRYLVE+EADFVLANDANDENR VIVKNLSKVAE FG+ RRW+VTNCDANRVM+
Sbjct: 98  GCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMT 157

Query: 124 EFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYG 183
           EFYL+KDFFDFIDVDSFGSDS+FLRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYG
Sbjct: 158 EFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYG 217

Query: 184 AYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKN 243
           AY+RPMPFSNEIGLRMLIGGVAREASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI+KN
Sbjct: 218 AYIRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKN 277

Query: 244 RHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDML 303
           RHYGFVSYC+KCGNSQAFSWSE+GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+L
Sbjct: 278 RHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDIL 337

Query: 304 TLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEM 363
           TLA QWEWIGNGQGKDLEKLL QM+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEM
Sbjct: 338 TLAKQWEWIGNGQGKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEM 397

Query: 364 IKAGYAASRSHIASNAIKTNCPMTECIRIALELQQSCLGVHQV 407
           IKAGYAASRSHIASNAIKTNCPM +CIRIA +LQQ+CLGVHQV
Sbjct: 398 IKAGYAASRSHIASNAIKTNCPMADCIRIA-QLQQNCLGVHQV 439

BLAST of Cp4.1LG01g11850 vs. NCBI nr
Match: gi|778701515|ref|XP_011655038.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus])

HSP 1 Score: 749.6 bits (1934), Expect = 3.3e-213
Identity = 365/398 (91.71%), Postives = 384/398 (96.48%), Query Frame = 1

Query: 9   LNNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIR 68
           LNNCL NNRTERG++FDIGDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIR
Sbjct: 43  LNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIR 102

Query: 69  SLRYLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQ 128
           SLRYLVE+EADFVLANDANDENR VIVKNLS+VAE  G+ RRW+VTNCDANRVM+EFYL+
Sbjct: 103 SLRYLVESEADFVLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLK 162

Query: 129 KDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRP 188
           KDFFDFIDVDSFGSDS+FLRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRP
Sbjct: 163 KDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRP 222

Query: 189 MPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGF 248
           MPFSNEIGLRMLIGGVAREASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGF
Sbjct: 223 MPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGF 282

Query: 249 VSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQ 308
           VSYC+KCGNSQAFSWSE+GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA Q
Sbjct: 283 VSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQ 342

Query: 309 WEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGY 368
           WEWIGNGQGKDLEKLL QMVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGY
Sbjct: 343 WEWIGNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGY 402

Query: 369 AASRSHIASNAIKTNCPMTECIRIALELQQSCLGVHQV 407
           AASRSHIASNAIKTNCPM ECIRIA +LQ++CLG HQV
Sbjct: 403 AASRSHIASNAIKTNCPMAECIRIA-QLQRNCLGAHQV 439

BLAST of Cp4.1LG01g11850 vs. NCBI nr
Match: gi|659114167|ref|XP_008456937.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 683.3 bits (1762), Expect = 2.9e-193
Identity = 330/362 (91.16%), Postives = 349/362 (96.41%), Query Frame = 1

Query: 4   SQIKSLNNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALC 63
           S  KS NNCL NNRTERG++FDIGDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALC
Sbjct: 38  SNKKSPNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALC 97

Query: 64  GCGIRSLRYLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMS 123
           GCGIRSLRYLVE+EADFVLANDANDENR VIVKNLSKVAE FG+ RRW+VTNCDANRVM+
Sbjct: 98  GCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMT 157

Query: 124 EFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYG 183
           EFYL+KDFFDFIDVDSFGSDS+FLRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYG
Sbjct: 158 EFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYG 217

Query: 184 AYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKN 243
           AY+RPMPFSNEIGLRMLIGGVAREASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI+KN
Sbjct: 218 AYIRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKN 277

Query: 244 RHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDML 303
           RHYGFVSYC+KCGNSQAFSWSE+GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+L
Sbjct: 278 RHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDIL 337

Query: 304 TLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEM 363
           TLA QWEWIGNGQGKDLEKLL QM+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEM
Sbjct: 338 TLAKQWEWIGNGQGKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEM 397

Query: 364 IK 366
           IK
Sbjct: 398 IK 399

BLAST of Cp4.1LG01g11850 vs. NCBI nr
Match: gi|224126557|ref|XP_002319867.1| (N2 family protein [Populus trichocarpa])

HSP 1 Score: 599.7 bits (1545), Expect = 4.2e-168
Identity = 286/387 (73.90%), Postives = 331/387 (85.53%), Query Frame = 1

Query: 12  CLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLR 71
           C    ++ERG+EF+ G+TFFRHESA GRDLGVLAA+LY +SKG LRVLDALCGCGIRSLR
Sbjct: 35  CKSELQSERGLEFETGETFFRHESAKGRDLGVLAASLYNQSKGKLRVLDALCGCGIRSLR 94

Query: 72  YLVEAEADFVLANDANDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDF 131
           YL+EA+ADFVLANDANDE+R VI++NL +V  GFG+ R+W+VT+ DANRV+SE YLQKDF
Sbjct: 95  YLMEAKADFVLANDANDEHRRVILENLKRVDRGFGDERKWVVTHLDANRVLSECYLQKDF 154

Query: 132 FDFIDVDSFGSD-STFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMP 191
           FD ID+DSFGSD S FLR A+N L  DGLLYVTSTDG SSGGHHP  SLAAYGAYVRPMP
Sbjct: 155 FDLIDIDSFGSDISLFLRSAMNTLSFDGLLYVTSTDGHSSGGHHPDHSLAAYGAYVRPMP 214

Query: 192 FSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVS 251
           +SNE+GLRMLIGG  REASVLG+Y+TPLFSYYSYHGPVFRVMLR++RG++ +NRHYGF++
Sbjct: 215 YSNEVGLRMLIGGAVREASVLGYYITPLFSYYSYHGPVFRVMLRVNRGKLLENRHYGFIT 274

Query: 252 YCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWE 311
           YC  CGNSQ FSW+E+GQ+SC C+ +  S SLV+SGPLWTGPLHSA YI  M+ LA +W 
Sbjct: 275 YCNNCGNSQEFSWAELGQISCSCSGSHGSRSLVISGPLWTGPLHSAAYISKMINLAEEWG 334

Query: 312 WIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAA 371
           WIGNG   DLEKLL QM+DESD +L  GYIKMDEVASRAK+NSPPL TMMS M K GYA 
Sbjct: 335 WIGNGAETDLEKLLKQMLDESDPRLKSGYIKMDEVASRAKINSPPLRTMMSAMQKEGYAT 394

Query: 372 SRSHIASNAIKTNCPMTECIRIALELQ 398
           SRSHIASNAIKT+CPM+ CIRI+ ELQ
Sbjct: 395 SRSHIASNAIKTDCPMSVCIRISKELQ 421

BLAST of Cp4.1LG01g11850 vs. NCBI nr
Match: gi|1009121668|ref|XP_015877582.1| (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2 [Ziziphus jujuba])

HSP 1 Score: 598.6 bits (1542), Expect = 9.4e-168
Identity = 284/391 (72.63%), Postives = 335/391 (85.68%), Query Frame = 1

Query: 11  NCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSL 70
           +C    ++ERG++F+ G++FFRHESATGRDLGVLAA+LYRKSKG+LRVLDALCGCG+RSL
Sbjct: 51  SCKSEYQSERGLQFETGESFFRHESATGRDLGVLAASLYRKSKGNLRVLDALCGCGVRSL 110

Query: 71  RYLVEAEADFVLANDANDENRGVIVKNLSK-VAEGFGETRRWMVTNCDANRVMSEFYLQK 130
           RYLVEAEADFVLANDAN+E   VI KNLS+ V  G G+  RW VTN DANR+M+E YLQ+
Sbjct: 111 RYLVEAEADFVLANDANEEYNSVICKNLSRMVPSGPGDECRWRVTNSDANRIMTECYLQR 170

Query: 131 DFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPM 190
           DFFDFIDVDSFGSDS+FLR A+NAL+LDGLLYVTSTDGFSSGGH P  SLAAYGAY++P+
Sbjct: 171 DFFDFIDVDSFGSDSSFLRSAMNALRLDGLLYVTSTDGFSSGGHRPHHSLAAYGAYIQPL 230

Query: 191 PFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFV 250
           P+SNE+GLRMLIGG  REASVLG+ V PLFSYY+YHGPVFRV+LR++RG++  NRHYGF+
Sbjct: 231 PYSNEVGLRMLIGGAVREASVLGYRVAPLFSYYAYHGPVFRVLLRVNRGKLPDNRHYGFI 290

Query: 251 SYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQW 310
           SYC++CGNSQAFSW E+GQ+SC C +++VSES++VSGPLWTG LH   Y+++ML LA +W
Sbjct: 291 SYCEQCGNSQAFSWDELGQISCLCTNSKVSESVIVSGPLWTGSLHDTVYVQEMLNLAEEW 350

Query: 311 EWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYA 370
            W G      LEK+L QMVDESD +LP GYIK+DEVASRAK+NSPPL TMMS + K GYA
Sbjct: 351 GWTGRDTETSLEKVLKQMVDESDPRLPFGYIKLDEVASRAKINSPPLRTMMSAIQKEGYA 410

Query: 371 ASRSHIASNAIKTNCPMTECIRIALELQQSC 401
           ASRSHI SNAIKTNCPMT CIRIA ELQQ C
Sbjct: 411 ASRSHIVSNAIKTNCPMTVCIRIAKELQQCC 441