Carg26921 (gene) Silver-seed gourd

Overview
NameCarg26921
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCalcium-binding EF-hand
LocationCucurbita_argyrosperma_scaffold_323 : 24842 .. 25306 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGAAGTCGGTTTCGAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTATTAGCGAGCTTGGCGCCGCCCTCGGCGAACTCGGCGGCAGAATCTCCTCGGACGAGATCCGCCGCATTATGTTAGAGATCGATACGGATGGCGATGGATTCATCGACCTTGACGAGTTCACCGCGTTTCATCAGGACGCCTATCCAGAGGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

mRNA sequence

ATGGCGAAGAAGTCGGTTTCGAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTATTAGCGAGCTTGGCGCCGCCCTCGGCGAACTCGGCGGCAGAATCTCCTCGGACGAGATCCGCCGCATTATGTTAGAGATCGATACGGATGGCGATGGATTCATCGACCTTGACGAGTTCACCGCGTTTCATCAGGACGCCTATCCAGAGGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

Coding sequence (CDS)

ATGGCGAAGAAGTCGGTTTCGAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTATTAGCGAGCTTGGCGCCGCCCTCGGCGAACTCGGCGGCAGAATCTCCTCGGACGAGATCCGCCGCATTATGTTAGAGATCGATACGGATGGCGATGGATTCATCGACCTTGACGAGTTCACCGCGTTTCATCAGGACGCCTATCCAGAGGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

Protein sequence

MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELGGRISSDEIRRIMLEIDTDGDGFIDLDEFTAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Homology
[Close]
BLAST of Carg26921 vs. NCBI nr
Match: XP_022934113.1 (calcium-binding protein CML24-like [Cucurbita moschata])

HSP 1 Score: 241.9 bits (616), Expect = 1.4e-60
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0 

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXX 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXX
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           XXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  XXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

[Close]
BLAST of Carg26921 vs. NCBI nr
Match: XP_023526443.1 (calcium-binding protein CML24-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 241.9 bits (616), Expect = 1.4e-60
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0 

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXX 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXX
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           XXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  XXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

[Close]
BLAST of Carg26921 vs. NCBI nr
Match: XP_022983538.1 (calcium-binding protein CML24-like [Cucurbita maxima])

HSP 1 Score: 235.3 bits (599), Expect = 1.3e-58
Identity = 148/154 (96.10%), Postives = 152/154 (98.70%), Query Frame = 0 

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXX 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKIS+SELGAALGEL XXXXXXXXXXXXXX
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGXXXXXXXXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           XXXXXXXXXXXXXXX FH++AYP+GGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  XXXXXXXXXXXXXXXXFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

[Close]
BLAST of Carg26921 vs. NCBI nr
Match: XP_022143269.1 (probable calcium-binding protein CML23 [Momordica charantia])

HSP 1 Score: 206.8 bits (525), Expect = 5.1e-50
Identity = 137/160 (85.62%), Postives = 143/160 (89.38%), Query Frame = 0 

Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXX 60
           MAKKSVS+P      GALGSMDEV K+F KFDKNGDGKIS++ELG ALGEL  XXXXXXX
Sbjct: 3   MAKKSVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGXXXXXXX 62

Query: 61  XXXXXXXXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           XXXXXXXXXXXXXXXXXXXXX FHQD    GGGNKDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  XXXXXXXXXXXXXXXXXXXXXXFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]
BLAST of Carg26921 vs. NCBI nr
Match: XP_022948508.1 (probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 probable calcium-binding protein CML23 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.4 bits (516), Expect = 5.7e-49
Identity = 134/160 (83.75%), Postives = 141/160 (88.12%), Query Frame = 0 

Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXX 60
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKISI EL AALGEL    XXXXX
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXX 62

Query: 61  XXXXXXXXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           XXXXXXXXXXXXXXXXXXXXX FHQ++   GGG+KDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  XXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]
BLAST of Carg26921 vs. TAIR10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 114.4 bits (285), Expect = 6.3e-26
Identity = 61/143 (42.66%), Postives = 79/143 (55.24%), Query Frame = 0 

Query: 12  LGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXX 71
           + + +E+ K+F +FD NGDGKIS+ ELG     +                          
Sbjct: 18  MANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINL 77

Query: 72  XXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMI 131
                  + +        +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI
Sbjct: 78  DEFSTLCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMI 137

Query: 132 SSVDVDGDGHVNFEEFKKMMSRS 155
             VD DGDG+VNFEEF+KMM+ S
Sbjct: 138 GPVDADGDGNVNFEEFQKMMTSS 156

[Close]
BLAST of Carg26921 vs. TAIR10
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 107.5 bits (267), Expect = 7.7e-24
Identity = 84/136 (61.76%), Postives = 99/136 (72.79%), Query Frame = 0 

Query: 17  EVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXA 76
           E+ K+F KFD NGDGKIS+SELG     +     XXXXXXXXXXXXXXXXXXXXXXXXX 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSM----GXXXXXXXXXXXXXXXXXXXXXXXXXX 79

Query: 77  FHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136
                        ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC RMI  VD 
Sbjct: 80  XXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 137 DGDGHVNFEEFKKMMS 153
           DGDG+VNFEEF+KMMS
Sbjct: 140 DGDGNVNFEEFQKMMS 151

[Close]
BLAST of Carg26921 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 102.1 bits (253), Expect = 3.2e-22
Identity = 55/136 (40.44%), Postives = 75/136 (55.15%), Query Frame = 0 

Query: 17  EVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXA 76
           E+  +FKKFD NGDGKIS  ELGA +  L                               
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 77  FHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136
            +     +    ++L+DAF +YD+D NG ISA+ELH VL+ LG++CS+ +C +MI  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 137 DGDGHVNFEEFKKMMS 153
           DGDG ++FEEFK MM+
Sbjct: 157 DGDGTIDFEEFKIMMT 172

[Close]
BLAST of Carg26921 vs. TAIR10
Match: AT1G66400.1 (calmodulin like 23)

HSP 1 Score: 97.8 bits (242), Expect = 6.1e-21
Identity = 88/150 (58.67%), Postives = 103/150 (68.67%), Query Frame = 0 

Query: 4   KSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGEL--XXXXXXXXXXXXXXXX 63
           K+VS    LGSM+++ K+F++FDKN DGKISI EL   +G L    XXXXXXXXXXXXXX
Sbjct: 3   KNVSR-NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAXXXXXXXXXXXXXX 62

Query: 64  XXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEK 123
           XXXXXXXXXXXXXX                        +D+NG ISA ELH V+K LGEK
Sbjct: 63  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDRNGRISANELHSVMKNLGEK 122

Query: 124 CSLKDCCRMISSVDVDGDGHVNFEEFKKMM 152
           CS++DC RMI+ VD DGDG V+FEEFKKMM
Sbjct: 123 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Carg26921 vs. TAIR10
Match: AT2G36180.1 (EF hand calcium-binding protein family)

HSP 1 Score: 90.5 bits (223), Expect = 9.7e-19
Identity = 56/139 (40.29%), Postives = 70/139 (50.36%), Query Frame = 0 

Query: 20  KIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAFHQ 79
           +IF+  DKN DGKI   E   A   +                               F  
Sbjct: 3   EIFESVDKNKDGKILWDEFAEA---IRVFSPQITSEEIDKMFIVLDVDGDGQIDDVEFAS 62

Query: 80  DAYPEGGGNKD------LQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISS 139
                GGG KD      +++AFDLYDMD +G ISA E+H VLKRLGEK +++DC  M+ +
Sbjct: 63  CLMVNGGGEKDTEEEVVMKEAFDLYDMDGDGKISASEIHVVLKRLGEKHTMEDCVVMVQT 122

Query: 140 VDVDGDGHVNFEEFKKMMS 153
           VD D DG VNFEEFK MM+
Sbjct: 123 VDKDSDGFVNFEEFKIMMN 138

[Close]
BLAST of Carg26921 vs. Swiss-Prot
Match: sp|Q9M7R0|ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-24
Identity = 63/140 (45.00%), Postives = 77/140 (55.00%), Query Frame = 0 

Query: 16  DEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 75
           +EV  +F +FD NGDGKIS  EL   L  L                              
Sbjct: 19  NEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFA 78

Query: 76  AF---HQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMIS 135
           AF     D YP  GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ +  DC  MI 
Sbjct: 79  AFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIK 138

Query: 136 SVDVDGDGHVNFEEFKKMMS 153
           SVD DGDG+V+FEEFKKMM+
Sbjct: 139 SVDSDGDGYVSFEEFKKMMT 158

[Close]
BLAST of Carg26921 vs. Swiss-Prot
Match: sp|Q9LE22|CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-24
Identity = 61/143 (42.66%), Postives = 79/143 (55.24%), Query Frame = 0 

Query: 12  LGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXX 71
           + + +E+ K+F +FD NGDGKIS+ ELG     +                          
Sbjct: 18  MANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINL 77

Query: 72  XXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMI 131
                  + +        +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI
Sbjct: 78  DEFSTLCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMI 137

Query: 132 SSVDVDGDGHVNFEEFKKMMSRS 155
             VD DGDG+VNFEEF+KMM+ S
Sbjct: 138 GPVDADGDGNVNFEEFQKMMTSS 156

[Close]
BLAST of Carg26921 vs. Swiss-Prot
Match: sp|Q0DJV6|CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.3e-24
Identity = 63/149 (42.28%), Postives = 80/149 (53.69%), Query Frame = 0 

Query: 15  MDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 74
           M E+ ++F+++D NGDGKIS  EL + L  L                             
Sbjct: 3   MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

Query: 75  XAFHQDAYPEGGG------------NKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKC 134
            AFH    P  GG              +L++AF +YD D NG ISA+ELH VL++LG+KC
Sbjct: 63  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 122

Query: 135 SLKDCCRMISSVDVDGDGHVNFEEFKKMM 152
           S+ DC RMI SVD DGDG VNF+EFKKMM
Sbjct: 123 SVADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of Carg26921 vs. Swiss-Prot
Match: sp|Q9C9U8|CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.4e-22
Identity = 84/136 (61.76%), Postives = 99/136 (72.79%), Query Frame = 0 

Query: 17  EVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXA 76
           E+ K+F KFD NGDGKIS+SELG     +     XXXXXXXXXXXXXXXXXXXXXXXXX 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSM----GXXXXXXXXXXXXXXXXXXXXXXXXXX 79

Query: 77  FHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136
                        ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC RMI  VD 
Sbjct: 80  XXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 137 DGDGHVNFEEFKKMMS 153
           DGDG+VNFEEF+KMMS
Sbjct: 140 DGDGNVNFEEFQKMMS 151

[Close]
BLAST of Carg26921 vs. Swiss-Prot
Match: sp|Q9FYK2|CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 5.8e-21
Identity = 55/136 (40.44%), Postives = 75/136 (55.15%), Query Frame = 0 

Query: 17  EVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXA 76
           E+  +FKKFD NGDGKIS  ELGA +  L                               
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 77  FHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136
            +     +    ++L+DAF +YD+D NG ISA+ELH VL+ LG++CS+ +C +MI  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 137 DGDGHVNFEEFKKMMS 153
           DGDG ++FEEFK MM+
Sbjct: 157 DGDGTIDFEEFKIMMT 172

[Close]
BLAST of Carg26921 vs. TrEMBL
Match: tr|A0A1S3C5W9|A0A1S3C5W9_CUCME (probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.1e-44
Identity = 126/152 (82.89%), Postives = 135/152 (88.82%), Query Frame = 0 

Query: 5   SVSNPG--ALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXX 64
           S+S PG  +LGSMDEV+++F KFDKNGDGKIS+SELGAALGEL    XXXXXXXXXXXXX
Sbjct: 9   SISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNIXXXXXXXXXXXXX 68

Query: 65  XXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKC 124
           XXXXXXXXXXXXX F   +    GGNKDLQDAFDLYD+DKNGLISAKELH VLKRLGEKC
Sbjct: 69  XXXXXXXXXXXXXDFTSSS---TGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKC 128

Query: 125 SLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           SLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 129 SLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

[Close]
BLAST of Carg26921 vs. TrEMBL
Match: tr|A0A0A0LCM1|A0A0A0LCM1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.5e-42
Identity = 121/152 (79.61%), Postives = 130/152 (85.53%), Query Frame = 0 

Query: 5   SVSNPG--ALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXX 64
           S+S PG  ALGSMDE++++F KFDKNGDGKIS++EL AALGEL      XXXXXXXXXXX
Sbjct: 8   SISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGEL---SGNXXXXXXXXXXX 67

Query: 65  XXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKC 124
           XXXXXXXXXXXXX          GGNKDLQDAFDLYD+DKNGLISAKELH VLKRLGEKC
Sbjct: 68  XXXXXXXXXXXXXXXXXXXXXXTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKC 127

Query: 125 SLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           SLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 128 SLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

[Close]
BLAST of Carg26921 vs. TrEMBL
Match: tr|A0A2P5E778|A0A2P5E778_9ROSA (Parvalbumin OS=Trema orientalis OX=63057 GN=TorRG33x02_228270 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.3e-33
Identity = 100/164 (60.98%), Postives = 112/164 (68.29%), Query Frame = 0 

Query: 1   MAKKS--VSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXX 60
           MAKKS   S P +LG+MD+V K+F KFD NGDG+IS  EL   L  L        XXXXX
Sbjct: 1   MAKKSSASSEPSSLGTMDDVRKVFNKFDSNGDGRISCDELRGMLLALGSKTSTEEXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXAFHQDAYPE---------GGGNKDLQDAFDLYDMDKNGLISAK 120
           XXXXXXXXXX        FH  +  +         G GNK+L+DAFDLYD+DKNGLISA 
Sbjct: 61  XXXXXXXXXXIDLKEFAEFHSGSEADQCGGAGAAAGAGNKELKDAFDLYDLDKNGLISAS 120

Query: 121 ELHFVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSR 154
           ELH V+KRLGEKCSL DC RMI SVD DGDG VNFEEFKKMM+R
Sbjct: 121 ELHAVMKRLGEKCSLSDCSRMIRSVDADGDGSVNFEEFKKMMTR 164

[Close]
BLAST of Carg26921 vs. TrEMBL
Match: tr|A0A2P5BY36|A0A2P5BY36_PARAD (Parvalbumin OS=Parasponia andersonii OX=3476 GN=PanWU01x14_200320 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.9e-33
Identity = 102/164 (62.20%), Postives = 112/164 (68.29%), Query Frame = 0 

Query: 1   MAKKS--VSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXX 60
           MAKKS   S P +LG+MD+V K+F KFD NGDG+IS  EL   L  L       XXXXXX
Sbjct: 1   MAKKSSVSSEPSSLGTMDDVRKVFNKFDSNGDGRISCDELRGMLLALGSKTSTEXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXAFHQ---------DAYPEGGGNKDLQDAFDLYDMDKNGLISAK 120
           XXXXXXXXXXX       FH          D      GNK+L+DAFDLYD+DKNGLISA 
Sbjct: 61  XXXXXXXXXXXDLKEFAEFHSGSEADQCGGDGAAAAAGNKELKDAFDLYDLDKNGLISAS 120

Query: 121 ELHFVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSR 154
           ELH V+KRLGEKCSL DC RMI SVD DGDG VNFEEFKKMM+R
Sbjct: 121 ELHAVMKRLGEKCSLSDCSRMIRSVDADGDGSVNFEEFKKMMTR 164

[Close]
BLAST of Carg26921 vs. TrEMBL
Match: tr|A0A166AVJ5|A0A166AVJ5_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_010743 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-32
Identity = 100/142 (70.42%), Postives = 114/142 (80.28%), Query Frame = 0 

Query: 13  GSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXXXXXXXXXXXXXXXXXXXX 72
           G M+EV K+F KFD NGDGKI ++ELG+ L  L      XXXXXXXXXXXXXXXXXXXXX
Sbjct: 10  GDMEEVKKVFNKFDTNGDGKIDLTELGSILRALGTNAPHXXXXXXXXXXXXXXXXXXXXX 69

Query: 73  XXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMIS 132
           XXX FH     +    KDL+DAFDLYD+DKNGLIS  ELH VLKRLGEKCSLKDC +M+S
Sbjct: 70  XXXDFHLGGSDDAANLKDLRDAFDLYDLDKNGLISVSELHAVLKRLGEKCSLKDCKKMVS 129

Query: 133 SVDVDGDGHVNFEEFKKMMSRS 155
           SVDVDGDG+VNFEEFKKMM+++
Sbjct: 130 SVDVDGDGNVNFEEFKKMMNKA 151

The following BLAST results are available for this feature:
BLAST of Carg26921 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022934113.11.4e-60100.00calcium-binding protein CML24-like [Cucurbita moschata][more]
XP_023526443.11.4e-60100.00calcium-binding protein CML24-like [Cucurbita pepo subsp. pepo][more]
XP_022983538.11.3e-5896.10calcium-binding protein CML24-like [Cucurbita maxima][more]
XP_022143269.15.1e-5085.63probable calcium-binding protein CML23 [Momordica charantia][more]
XP_022948508.15.7e-4983.75probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 prob... [more]
BLAST of Carg26921 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.16.3e-2642.66Calcium-binding EF-hand family protein[more]
AT1G73630.17.7e-2461.76EF hand calcium-binding protein family[more]
AT1G24620.13.2e-2240.44EF hand calcium-binding protein family[more]
AT1G66400.16.1e-2158.67calmodulin like 23[more]
AT2G36180.19.7e-1940.29EF hand calcium-binding protein family[more]
BLAST of Carg26921 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9M7R0|ALL8_OLEEU1.1e-2445.00Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1[more]
sp|Q9LE22|CML27_ARATH1.1e-2442.66Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ... [more]
sp|Q0DJV6|CML18_ORYSJ3.3e-2442.28Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9C9U8|CML26_ARATH1.4e-2261.76Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ... [more]
sp|Q9FYK2|CML25_ARATH5.8e-2140.44Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
BLAST of Carg26921 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3C5W9|A0A1S3C5W9_CUCME2.1e-4482.89probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 P... [more]
tr|A0A0A0LCM1|A0A0A0LCM1_CUCSA1.5e-4279.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1[more]
tr|A0A2P5E778|A0A2P5E778_9ROSA1.3e-3360.98Parvalbumin OS=Trema orientalis OX=63057 GN=TorRG33x02_228270 PE=4 SV=1[more]
tr|A0A2P5BY36|A0A2P5BY36_PARAD2.9e-3362.20Parvalbumin OS=Parasponia andersonii OX=3476 GN=PanWU01x14_200320 PE=4 SV=1[more]
tr|A0A166AVJ5|A0A166AVJ5_DAUCA1.1e-3270.42Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_010743 ... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26921-RACarg26921-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 126..154
e-value: 2.4E-4
score: 30.5
coord: 53..81
e-value: 0.0018
score: 27.5
coord: 90..118
e-value: 1.2E-4
score: 31.5
coord: 17..45
e-value: 4.2E-7
score: 39.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 17..77
e-value: 2.1E-13
score: 50.5
coord: 89..151
e-value: 3.1E-10
score: 40.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 122..154
score: 13.063
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 86..121
score: 13.677
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 49..84
score: 13.537
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 13..48
score: 16.159
IPR002048EF-hand domainCDDcd00051EFhcoord: 90..152
e-value: 2.67364E-17
score: 71.0397
IPR002048EF-hand domainCDDcd00051EFhcoord: 17..79
e-value: 2.70137E-17
score: 71.0397
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 87..154
e-value: 3.4E-22
score: 80.4
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 3..85
e-value: 2.6E-21
score: 78.1
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 5..154
NoneNo IPR availablePANTHERPTHR10891:SF698CALCIUM-BINDING PROTEIN CML26-RELATEDcoord: 5..154
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 62..74
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 26..38
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 99..111
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 135..147
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 15..153