Carg07449 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg07449
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Calcium-binding EF-hand
Location	Cucurbita_argyrosperma_scaffold_019 : 898212 .. 898700 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTATGGCGAAGACGTCGGTTTCAAATCCAAGCTCCCCAAATCCTGGAGGAGCCTTGGGCTCCATGGACGAGGTTATCATGGTCTTCAAGAAGTTTGATAAAAACGGCGATGGCAAGATTTCCATCAAGGAGCTCGCTGCCGCCCTTGGGGAACTTGGCGGCAAGATCTCTGACGATGAGATCCGCCGCATTATGTCGGAGATTGACAAGGACGGTAATGGATTCATCGACCTAGACGAGTTCGCCGAGTTTCATCAGAACTCTTCTTCTACTGGCGGAGGTAGCAAAGATCTGCAAGACGCTTTTGATCTGTACGATGTCGATAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTCTGTTCTCAAGCGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGCTCCGTCGATGTTGACGGTGACGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGACTCGCTCATAG

mRNA sequence

ATGCCTATGGCGAAGACGTCGGTTTCAAATCCAAGCTCCCCAAATCCTGGAGGAGCCTTGGGCTCCATGGACGAGGTTATCATGGTCTTCAAGAAGTTTGATAAAAACGGCGATGGCAAGATTTCCATCAAGGAGCTCGCTGCCGCCCTTGGGGAACTTGGCGGCAAGATCTCTGACGATGAGATCCGCCGCATTATGTCGGAGATTGACAAGGACGGTAATGGATTCATCGACCTAGACGAGTTCGCCGAGTTTCATCAGAACTCTTCTTCTACTGGCGGAGGTAGCAAAGATCTGCAAGACGCTTTTGATCTGTACGATGTCGATAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTCTGTTCTCAAGCGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGCTCCGTCGATGTTGACGGTGACGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGACTCGCTCATAG

Coding sequence (CDS)

ATGCCTATGGCGAAGACGTCGGTTTCAAATCCAAGCTCCCCAAATCCTGGAGGAGCCTTGGGCTCCATGGACGAGGTTATCATGGTCTTCAAGAAGTTTGATAAAAACGGCGATGGCAAGATTTCCATCAAGGAGCTCGCTGCCGCCCTTGGGGAACTTGGCGGCAAGATCTCTGACGATGAGATCCGCCGCATTATGTCGGAGATTGACAAGGACGGTAATGGATTCATCGACCTAGACGAGTTCGCCGAGTTTCATCAGAACTCTTCTTCTACTGGCGGAGGTAGCAAAGATCTGCAAGACGCTTTTGATCTGTACGATGTCGATAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTCTGTTCTCAAGCGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGCTCCGTCGATGTTGACGGTGACGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGACTCGCTCATAG

Protein sequence

MPMAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEIRRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS

Homology

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BLAST of Carg07449 vs. NCBI nr
Match: XP_022948508.1 (probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 probable calcium-binding protein CML23 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 264.2 bits (674), Expect = 2.8e-67
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0


Query: 1   MPMAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXX 60
           MPMAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXX
Sbjct: 1   MPMAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELH 120
           XXXXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELH
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELH 120

Query: 121 SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

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BLAST of Carg07449 vs. NCBI nr
Match: XP_022998716.1 (probable calcium-binding protein CML23 [Cucurbita maxima])

HSP 1 Score: 260.4 bits (664), Expect = 4.1e-66
Identity = 160/162 (98.77%), Postives = 161/162 (99.38%), Query Frame = 0


Query: 1   MPMAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXX 60
           MPMAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXX
Sbjct: 1   MPMAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELH 120
           XXXXXXXXXXXXXXXXXXXXXXXEFHQN SSTGGGSKDLQDAFDL+DVDKNGLISAKELH
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXEFHQNFSSTGGGSKDLQDAFDLFDVDKNGLISAKELH 120

Query: 121 SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

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BLAST of Carg07449 vs. NCBI nr
Match: XP_022143269.1 (probable calcium-binding protein CML23 [Momordica charantia])

HSP 1 Score: 227.3 bits (578), Expect = 3.9e-56
Identity = 143/162 (88.27%), Postives = 149/162 (91.98%), Query Frame = 0


Query: 1   MPMAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXX 60
           MPMAK SVS+PSS N GGALGSMDEV  VF KFDKNGDGKIS+ EL  ALGELGG  XXX
Sbjct: 1   MPMAKKSVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELH 120
           XXXXXXXXXXXXXXXXXXXXXXX FHQ+ S+TGGG+KDLQDAFDLYD+DKNGLISAKELH
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELH 120

Query: 121 SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 SVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

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BLAST of Carg07449 vs. NCBI nr
Match: XP_022983538.1 (calcium-binding protein CML24-like [Cucurbita maxima])

HSP 1 Score: 203.8 bits (517), Expect = 4.6e-49
Identity = 133/160 (83.12%), Postives = 141/160 (88.12%), Query Frame = 0


Query: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXX 62
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKIS+ EL AALGELG   XXXXX
Sbjct: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGXXXXXXXX 60

Query: 63  XXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122
           XXXXXXXXXXXXXXXXXXXXX FH+ +   GGG+KDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120

Query: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

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BLAST of Carg07449 vs. NCBI nr
Match: XP_022934113.1 (calcium-binding protein CML24-like [Cucurbita moschata])

HSP 1 Score: 203.4 bits (516), Expect = 6.0e-49
Identity = 134/160 (83.75%), Postives = 141/160 (88.12%), Query Frame = 0


Query: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXX 62
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKISI EL AALGEL    XXXXX
Sbjct: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISISELGAALGELXXXXXXXXX 60

Query: 63  XXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122
           XXXXXXXXXXXXXXXXXXXXX FHQ++   GGG+KDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120

Query: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

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BLAST of Carg07449 vs. TAIR10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 121.7 bits (304), Expect = 4.1e-28
Identity = 69/157 (43.95%), Postives = 88/157 (56.05%), Query Frame = 0


Query: 9   SNPSSPNPGGA---LGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXX 68
           +NP +  P  A   + + +E+  VF +FD NGDGKIS+ EL      +G           
Sbjct: 4   ANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRV 63

Query: 69  XXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKR 128
                                ++SSS    + +++DAFDLYD DKNGLISA ELH VL R
Sbjct: 64  LEEVDTDRDGYINLDEFSTLCRSSSS----AAEIRDAFDLYDQDKNGLISASELHQVLNR 123

Query: 129 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           LG  CS++DC RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 124 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Carg07449 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 112.1 bits (279), Expect = 3.3e-25
Identity = 59/136 (43.38%), Postives = 78/136 (57.35%), Query Frame = 0


Query: 25  EVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXXXXXXXXXXXXXXXXXXXE 84
           E+  VFKKFD NGDGKIS KEL A +  LG ++                          E
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 85  FHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 144
            +          ++L+DAF +YD+D NG ISA+ELH VL+ LG++CS+ +C +MI  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 145 DGDGHVNFEEFKKMMT 161
           DGDG ++FEEFK MMT
Sbjct: 157 DGDGTIDFEEFKIMMT 172

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BLAST of Carg07449 vs. TAIR10
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 102.8 bits (255), Expect = 2.0e-22
Identity = 85/152 (55.92%), Postives = 102/152 (67.11%), Query Frame = 0


Query: 9   SNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXXXX 68
           +N  S N      +  E+  VF KFD NGDGKIS+ EL      +G    XXXXXXXXXX
Sbjct: 4   TNLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMG----XXXXXXXXXX 63

Query: 69  XXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGE 128
           XXXXXXXXXXXXXXX              ++++AFDLYD +KNGLIS+ E+H VL RLG 
Sbjct: 64  XXXXXXXXXXXXXXXXXXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGM 123

Query: 129 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMT 161
            CS++DC RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 124 TCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151

[Close]

BLAST of Carg07449 vs. TAIR10
Match: AT1G66400.1 (calmodulin like 23)

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-20
Identity = 84/142 (59.15%), Postives = 97/142 (68.31%), Query Frame = 0


Query: 20  LGSMDEVIMVFKKFDKNGDGKISIKELAAALGEL--GGKIXXXXXXXXXXXXXXXXXXXX 79
           LGSM+++  VF++FDKN DGKISI EL   +G L      XXXXXXXXXXXXXXXXXXXX
Sbjct: 10  LGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAXXXXXXXXXXXXXXXXXXXXXX 69

Query: 80  XXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGEKCSLKDCCR 139
           XXXXXX                        +D+NG ISA ELHSV+K LGEKCS++DC R
Sbjct: 70  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDRNGRISANELHSVMKNLGEKCSIQDCQR 129

Query: 140 MISSVDVDGDGHVNFEEFKKMM 160
           MI+ VD DGDG V+FEEFKKMM
Sbjct: 130 MINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Carg07449 vs. TAIR10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 94.7 bits (234), Expect = 5.4e-20
Identity = 77/153 (50.33%), Postives = 103/153 (67.32%), Query Frame = 0


Query: 9   SNPSSPNPGG-ALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXXX 68
           S  SSP PGG +  S++E+  VF +FD + DGKIS  E    L  LG +     XXXXXX
Sbjct: 33  SGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQTEYKVVLRALGQERAIEDXXXXXX 92

Query: 69  XXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLG 128
           XXXXXXXXXXX     + ++ S   G  S D++++F  +D++ +G ISA+E+ SVL +LG
Sbjct: 93  XXXXXXXXXXXFREFIDAYKRSG--GIRSSDIRNSFWTFDLNGDGKISAEEVMSVLWKLG 152

Query: 129 EKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMT 161
           E+CSL+DC RM+ +VD DGDG VN EEF KMM+
Sbjct: 153 ERCSLEDCNRMVRAVDADGDGLVNMEEFIKMMS 183

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BLAST of Carg07449 vs. Swiss-Prot
Match: sp|Q9LE22|CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 7.5e-27
Identity = 69/157 (43.95%), Postives = 88/157 (56.05%), Query Frame = 0


Query: 9   SNPSSPNPGGA---LGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXX 68
           +NP +  P  A   + + +E+  VF +FD NGDGKIS+ EL      +G           
Sbjct: 4   ANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRV 63

Query: 69  XXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKR 128
                                ++SSS    + +++DAFDLYD DKNGLISA ELH VL R
Sbjct: 64  LEEVDTDRDGYINLDEFSTLCRSSSS----AAEIRDAFDLYDQDKNGLISASELHQVLNR 123

Query: 129 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           LG  CS++DC RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 124 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Carg07449 vs. Swiss-Prot
Match: sp|Q0DJV6|CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.4e-25
Identity = 66/149 (44.30%), Postives = 84/149 (56.38%), Query Frame = 0


Query: 23  MDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXXXXXXXXXXXXXXXXXX 82
           M E+  VF+++D NGDGKIS +ELA+ L  LG  +                         
Sbjct: 3   MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

Query: 83  XEFHQNSS----STGGGSKD--------LQDAFDLYDVDKNGLISAKELHSVLKRLGEKC 142
             FH   +      GG +KD        L++AF +YD D NG ISA+ELH VL++LG+KC
Sbjct: 63  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 122

Query: 143 SLKDCCRMISSVDVDGDGHVNFEEFKKMM 160
           S+ DC RMI SVD DGDG VNF+EFKKMM
Sbjct: 123 SVADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of Carg07449 vs. Swiss-Prot
Match: sp|Q9FYK2|CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.9e-24
Identity = 59/136 (43.38%), Postives = 78/136 (57.35%), Query Frame = 0


Query: 25  EVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXXXXXXXXXXXXXXXXXXXE 84
           E+  VFKKFD NGDGKIS KEL A +  LG ++                          E
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 85  FHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 144
            +          ++L+DAF +YD+D NG ISA+ELH VL+ LG++CS+ +C +MI  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 145 DGDGHVNFEEFKKMMT 161
           DGDG ++FEEFK MMT
Sbjct: 157 DGDGTIDFEEFKIMMT 172

[Close]

BLAST of Carg07449 vs. Swiss-Prot
Match: sp|Q9M7R0|ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.3e-23
Identity = 65/148 (43.92%), Postives = 80/148 (54.05%), Query Frame = 0


Query: 16  PGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXXXXXXXXXXX 75
           P   L   +EV  VF +FD NGDGKIS  ELA  L  LG                     
Sbjct: 11  PSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDG 70

Query: 76  XXXXXXXXEFHQNSSS---TGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGEKCSL 135
                    F +  +    + GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ + 
Sbjct: 71  FINVQEFAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAE 130

Query: 136 KDCCRMISSVDVDGDGHVNFEEFKKMMT 161
            DC  MI SVD DGDG+V+FEEFKKMMT
Sbjct: 131 HDCVEMIKSVDSDGDGYVSFEEFKKMMT 158

[Close]

BLAST of Carg07449 vs. Swiss-Prot
Match: sp|Q9C9U8|CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 3.6e-21
Identity = 85/152 (55.92%), Postives = 102/152 (67.11%), Query Frame = 0


Query: 9   SNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXXXX 68
           +N  S N      +  E+  VF KFD NGDGKIS+ EL      +G    XXXXXXXXXX
Sbjct: 4   TNLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMG----XXXXXXXXXX 63

Query: 69  XXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRLGE 128
           XXXXXXXXXXXXXXX              ++++AFDLYD +KNGLIS+ E+H VL RLG 
Sbjct: 64  XXXXXXXXXXXXXXXXXXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGM 123

Query: 129 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMT 161
            CS++DC RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 124 TCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151

[Close]

BLAST of Carg07449 vs. TrEMBL
Match: tr|A0A1S3C5W9|A0A1S3C5W9_CUCME (probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 PE=4 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.3e-47
Identity = 131/156 (83.97%), Postives = 139/156 (89.10%), Query Frame = 0


Query: 7   SVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXX 66
           S+S P S     +LGSMDEVI VF KFDKNGDGKIS+ EL AALGEL G IXXXXXXXXX
Sbjct: 9   SISTPGS----SSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNIXXXXXXXXX 68

Query: 67  XXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRL 126
           XXXXXXXXXXXXXXXXX+F   +SS+ GG+KDLQDAFDLYD+DKNGLISAKELHSVLKRL
Sbjct: 69  XXXXXXXXXXXXXXXXXDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 128

Query: 127 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 129 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

[Close]

BLAST of Carg07449 vs. TrEMBL
Match: tr|A0A0A0LCM1|A0A0A0LCM1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-44
Identity = 129/160 (80.62%), Postives = 137/160 (85.62%), Query Frame = 0


Query: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXX 62
           MAK +  + S+P    ALGSMDE+I VF KFDKNGDGKIS+ ELAAALGEL G  XXXXX
Sbjct: 1   MAKNNSLSISTPG-SSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNXXXXXX 60

Query: 63  XXXXXXXXXXXXXXXXXXXXXEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122
           XXXXXXXXXXXXXXXXXXXXX      +   GG+KDLQDAFDLYD+DKNGLISAKELHSV
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXT---GGNKDLQDAFDLYDIDKNGLISAKELHSV 120

Query: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 163
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

[Close]

BLAST of Carg07449 vs. TrEMBL
Match: tr|A0A2P5E778|A0A2P5E778_9ROSA (Parvalbumin OS=Trema orientalis OX=63057 GN=TorRG33x02_228270 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.6e-34
Identity = 102/168 (60.71%), Postives = 116/168 (69.05%), Query Frame = 0


Query: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXX 62
           MAK S    S+ +   +LG+MD+V  VF KFD NGDG+IS  EL   L  LG K     X
Sbjct: 1   MAKKS----SASSEPSSLGTMDDVRKVFNKFDSNGDGRISCDELRGMLLALGSKTSTEEX 60

Query: 63  XXXXXXXXXXXXXXXXXXXXXEFHQNS---------SSTGGGSKDLQDAFDLYDVDKNGL 122
           XXXXXXXXXXXXXX       EFH  S         ++ G G+K+L+DAFDLYD+DKNGL
Sbjct: 61  XXXXXXXXXXXXXXIDLKEFAEFHSGSEADQCGGAGAAAGAGNKELKDAFDLYDLDKNGL 120

Query: 123 ISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTR 162
           ISA ELH+V+KRLGEKCSL DC RMI SVD DGDG VNFEEFKKMMTR
Sbjct: 121 ISASELHAVMKRLGEKCSLSDCSRMIRSVDADGDGSVNFEEFKKMMTR 164

[Close]

BLAST of Carg07449 vs. TrEMBL
Match: tr|A0A2P5BY36|A0A2P5BY36_PARAD (Parvalbumin OS=Parasponia andersonii OX=3476 GN=PanWU01x14_200320 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.0e-33
Identity = 103/166 (62.05%), Postives = 117/166 (70.48%), Query Frame = 0


Query: 5   KTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXX 64
           K+SVS+  S     +LG+MD+V  VF KFD NGDG+IS  EL   L  LG K    XXXX
Sbjct: 4   KSSVSSEPS-----SLGTMDDVRKVFNKFDSNGDGRISCDELRGMLLALGSKTSTEXXXX 63

Query: 65  XXXXXXXXXXXXXXXXXXXEFHQNS---------SSTGGGSKDLQDAFDLYDVDKNGLIS 124
           XXXXXXXXXXXXX      EFH  S         ++   G+K+L+DAFDLYD+DKNGLIS
Sbjct: 64  XXXXXXXXXXXXXDLKEFAEFHSGSEADQCGGDGAAAAAGNKELKDAFDLYDLDKNGLIS 123

Query: 125 AKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTR 162
           A ELH+V+KRLGEKCSL DC RMI SVD DGDG VNFEEFKKMMTR
Sbjct: 124 ASELHAVMKRLGEKCSLSDCSRMIRSVDADGDGSVNFEEFKKMMTR 164

[Close]

BLAST of Carg07449 vs. TrEMBL
Match: tr|W9S192|W9S192_9ROSA (Putative calcium-binding protein CML23 OS=Morus notabilis OX=981085 GN=L484_027232 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.8e-33
Identity = 80/146 (54.79%), Postives = 93/146 (63.70%), Query Frame = 0


Query: 19  ALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKIXXXXXXXXXXXXXXXXXXXXX 78
           +LGSM+EV  +F KFDKN DGKIS  EL   LG LG K                      
Sbjct: 5   SLGSMEEVQKIFNKFDKNRDGKISRSELRDVLGALGSKTSDDEVKRVMAEIDADGDGYID 64

Query: 79  XXXXXEFHQNSSSTGGGS--KDLQDAFDLYDVDKNGLISAKELHSVLKRLGEKCSLKDCC 138
                +FH   S   G +  K+L+DAFDLYD+DKNG+IS++ELHSVL+ LGEKCSL DC 
Sbjct: 65  FDEFVKFHSGGSENDGAANEKELRDAFDLYDLDKNGVISSRELHSVLRSLGEKCSLSDCS 124

Query: 139 RMISSVDVDGDGHVNFEEFKKMMTRS 163
           RMI SVD DGDG VNFEEFKKMMTR+
Sbjct: 125 RMIRSVDADGDGSVNFEEFKKMMTRA 150

The following BLAST results are available for this feature:

BLAST of Carg07449 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948508.1	2.8e-67	100.00	probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 prob...	[more]
XP_022998716.1	4.1e-66	98.77	probable calcium-binding protein CML23 [Cucurbita maxima]	[more]
XP_022143269.1	3.9e-56	88.27	probable calcium-binding protein CML23 [Momordica charantia]	[more]
XP_022983538.1	4.6e-49	83.13	calcium-binding protein CML24-like [Cucurbita maxima]	[more]
XP_022934113.1	6.0e-49	83.75	calcium-binding protein CML24-like [Cucurbita moschata]	[more]

BLAST of Carg07449 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G18210.1	4.1e-28	43.95	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	3.3e-25	43.38	EF hand calcium-binding protein family	[more]
AT1G73630.1	2.0e-22	55.92	EF hand calcium-binding protein family	[more]
AT1G66400.1	1.1e-20	59.15	calmodulin like 23	[more]
AT2G15680.1	5.4e-20	50.33	Calcium-binding EF-hand family protein	[more]

BLAST of Carg07449 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LE22\|CML27_ARATH	7.5e-27	43.95	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
sp\|Q0DJV6\|CML18_ORYSJ	2.4e-25	44.30	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q9FYK2\|CML25_ARATH	5.9e-24	43.38	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
sp\|Q9M7R0\|ALL8_OLEEU	1.3e-23	43.92	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
sp\|Q9C9U8\|CML26_ARATH	3.6e-21	55.92	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ...	[more]

BLAST of Carg07449 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3C5W9\|A0A1S3C5W9_CUCME	6.3e-47	83.97	probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 P...	[more]
tr\|A0A0A0LCM1\|A0A0A0LCM1_CUCSA	1.3e-44	80.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1	[more]
tr\|A0A2P5E778\|A0A2P5E778_9ROSA	3.6e-34	60.71	Parvalbumin OS=Trema orientalis OX=63057 GN=TorRG33x02_228270 PE=4 SV=1	[more]
tr\|A0A2P5BY36\|A0A2P5BY36_PARAD	1.0e-33	62.05	Parvalbumin OS=Parasponia andersonii OX=3476 GN=PanWU01x14_200320 PE=4 SV=1	[more]
tr\|W9S192\|W9S192_9ROSA	1.8e-33	54.79	Putative calcium-binding protein CML23 OS=Morus notabilis OX=981085 GN=L484_0272...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07449-RA	Carg07449-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 134..162 e-value: 2.1E-4 score: 30.6 coord: 98..126 e-value: 2.8E-5 score: 33.5 coord: 25..53 e-value: 7.9E-6 score: 35.4 coord: 61..89 e-value: 2.4E-5 score: 33.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 28..85 e-value: 5.4E-15 score: 55.5 coord: 97..159 e-value: 4.0E-12 score: 46.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 21..56 score: 14.904
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 130..162 score: 13.174
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 94..129 score: 14.569
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 57..92 score: 13.928
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 25..87 e-value: 4.26445E-19 score: 76.0473
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 98..160 e-value: 1.08468E-18 score: 74.8917
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 125..161 e-value: 8.5E-14 score: 53.2
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 64..124 e-value: 2.8E-18 score: 67.6
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 21..63 e-value: 1.9E-10 score: 42.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
None	No IPR available	PANTHER	PTHR10891:SF698	CALCIUM-BINDING PROTEIN CML26-RELATED	coord: 15..162
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..162
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 143..155
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 107..119
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 70..82
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 34..46
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 23..161

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg07449	Cla013188	Watermelon (97103) v1	carwmB0760
Carg07449	Csa3G319280	Cucumber (Chinese Long) v2	carcuB0780
Carg07449	MELO3C020263	Melon (DHL92) v3.5.1	carmeB0756
Carg07449	ClCG05G011200	Watermelon (Charleston Gray)	carwcgB0739
Carg07449	CSPI03G21520	Wild cucumber (PI 183967)	carcpiB0798
Carg07449	Cucsa.375430	Cucumber (Gy14) v1	carcgyB0866
Carg07449	CmaCh01G003620	Cucurbita maxima (Rimu)	carcmaB1059
Carg07449	CmaCh14G008380	Cucurbita maxima (Rimu)	carcmaB1057
Carg07449	CmoCh01G003790	Cucurbita moschata (Rifu)	carcmoB1033
Carg07449	CmoCh14G008530	Cucurbita moschata (Rifu)	carcmoB1031
Carg07449	Lsi05G003280	Bottle gourd (USVL1VR-Ls)	carlsiB700
Carg07449	Cp4.1LG03g05830	Cucurbita pepo (Zucchini)	carcpeB1073
Carg07449	Cp4.1LG02g16750	Cucurbita pepo (Zucchini)	carcpeB1072
Carg07449	CsGy3G020790	Cucumber (Gy14) v2	carcgybB0757
Carg07449	MELO3C020263.2	Melon (DHL92) v3.6.1	carmedB0771
Carg07449	CsaV3_3G021210	Cucumber (Chinese Long) v3	carcucB0801

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg07449	Carg26921	Silver-seed gourd	carcarB495

The following block(s) are covering this gene:


Gene	Organism	Block
Carg07449	Watermelon (97103) v2	carwmbB0795
Carg07449	Wax gourd	carwgoB0896