CSPI03G21520 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G21520
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	EF hand calcium-binding protein family
Location	Chr3 : 17810960 .. 17811872 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAGGTAAAAAAACCTCTCTCTATTCAAATTTTAAATCCTCTTCACAATCAACACCATGGCCAAGAACAACTCCCTTTCAATTTCTACTCCAGGATCCTCCGCCTTAGGCTCCATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAGTGTTCTTCGCCAGTAGAGAGGTAGTTTCACAAATTTAATCAAATTTAGGCTTTAGAATTTCGAGACCAAAATCGAGTATTATCGGTTCATTCTTAGTGTGTACAGTCCATGAATCTATCCTTTCTTTCACGGTTTGTAACCGATTTAGGGCACCAAACGCCTCTACAATGAATTTCTCCTTTGATTTTAGATCTTAATCGGAGATCGGAAAAGGAAAAAAAAAGAACAAACAGATTATAAGTTCATATCATCTCCCTCTAATTGATTCTGATTTTCTCTTTCTGAAACGGACGGAAGAATCCGAAGTTGGAGGAAGTAAATTTTGTTGAATATTTGATTGGTTGATGCTATAGTTATTGAAGCAATTATTTGCATCGGTTAG

mRNA sequence

ATGGCCAAGAACAACTCCCTTTCAATTTCTACTCCAGGATCCTCCGCCTTAGGCTCCATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAG

Coding sequence (CDS)

ATGGCCAAGAACAACTCCCTTTCAATTTCTACTCCAGGATCCTCCGCCTTAGGCTCCATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAG

Homology

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BLAST of CSPI03G21520 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 8.7e-41
Identity = 85/144 (59.03%), Postives = 106/144 (73.61%), Query Frame = 1


Query: 15  SALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFI 74
           + LGSM++I +VF +FDKN DGKIS+ EL   +G LS N S +E   +M E D DG+GFI
Sbjct: 8   NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFI 67

Query: 75  DLDEFTDF-----TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDC 134
           DLDEF         SS+    +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC
Sbjct: 68  DLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDC 127

Query: 135 CRMISSVDVDGDGHVNFEEFKKMM 154
            RMI+ VD DGDG V+FEEFKKMM
Sbjct: 128 QRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CSPI03G21520 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 167.2 bits (422), Expect = 1.5e-40
Identity = 86/147 (58.50%), Postives = 105/147 (71.43%), Query Frame = 1


Query: 15  SALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFI 74
           S LGSMD+I +VF +FDKNGDGKISV EL   +  LS   S +E   +M + D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 75  DLDEFTDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLK 134
           DLDEF        GG         DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 135 DCCRMISSVDVDGDGHVNFEEFKKMMT 155
           DC +MIS VD+DGDG VNF+EFKKMM+
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of CSPI03G21520 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.8e-38
Identity = 78/146 (53.42%), Postives = 105/146 (71.92%), Query Frame = 1


Query: 11  TPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDG 70
           TP +  + + +E+ +VF++FD NGDGKISV EL      +  + +  E++R++ E+D D 
Sbjct: 12  TPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDR 71

Query: 71  DGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCC 130
           DG+I+LDEF+    SS+    +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC 
Sbjct: 72  DGYINLDEFSTLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCT 131

Query: 131 RMISSVDVDGDGHVNFEEFKKMMTRS 157
           RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 132 RMIGPVDADGDGNVNFEEFQKMMTSS 156

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BLAST of CSPI03G21520 vs. Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 2.9e-36
Identity = 80/140 (57.14%), Postives = 98/140 (70.00%), Query Frame = 1


Query: 21  DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 80
           +E+  VFN+FD NGDGKIS  ELA  L  L  N S +EI RIM EID D DGFI++ EF 
Sbjct: 19  NEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFA 78

Query: 81  DFTSS------STGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMIS 140
            F  +      S+GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ +  DC  MI 
Sbjct: 79  AFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIK 138

Query: 141 SVDVDGDGHVNFEEFKKMMT 155
           SVD DGDG+V+FEEFKKMMT
Sbjct: 139 SVDSDGDGYVSFEEFKKMMT 158

[Close]

BLAST of CSPI03G21520 vs. Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 3.5e-34
Identity = 74/145 (51.03%), Postives = 103/145 (71.03%), Query Frame = 1


Query: 10  STPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKD 69
           ST  S+   +  E+ +VF+KFD NGDGKISV+EL      +  + + +E++R++ EID D
Sbjct: 8   STNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDID 67

Query: 70  GDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDC 129
            DGFI+ +EF     SS+    ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC
Sbjct: 68  CDGFINQEEFATICRSSSSA-VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDC 127

Query: 130 CRMISSVDVDGDGHVNFEEFKKMMT 155
            RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 128 VRMIGHVDTDGDGNVNFEEFQKMMS 151

[Close]

BLAST of CSPI03G21520 vs. TrEMBL
Match: A0A0A0LCM1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G319280 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 2.7e-81
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 1


Query: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60
           MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH
Sbjct: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60

Query: 61  RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 120
           RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL
Sbjct: 61  RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 120

Query: 121 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

[Close]

BLAST of CSPI03G21520 vs. TrEMBL
Match: A0A067F6W5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031791mg PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.5e-47
Identity = 102/158 (64.56%), Postives = 117/158 (74.05%), Query Frame = 1


Query: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60
           MA NNS      G++ LGSMDE+ +VFNKFDKNGDGKIS  EL   L  L    S +E+ 
Sbjct: 1   MAANNS-----NGANYLGSMDEVRKVFNKFDKNGDGKISADELKDVLRSLGSKTSPEEVK 60

Query: 61  RIMSEIDKDGDGFIDLDEFTDF--TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLK 120
           R+M EID DGDG+ID  EF+ F     ST G K+L+DAFDLYD+D+NGLISA ELH+VLK
Sbjct: 61  RVMDEIDTDGDGYIDFKEFSTFHLAGGSTDGTKELKDAFDLYDMDQNGLISANELHAVLK 120

Query: 121 RLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           +LGEK SLKDC  MI  VD DGDGHVNFEEFKKMMTRS
Sbjct: 121 KLGEKSSLKDCVNMIKKVDADGDGHVNFEEFKKMMTRS 153

[Close]

BLAST of CSPI03G21520 vs. TrEMBL
Match: V4UVY1_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10013012mg PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.5e-47
Identity = 102/158 (64.56%), Postives = 117/158 (74.05%), Query Frame = 1


Query: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60
           MA NNS      G++ LGSMDE+ +VFNKFDKNGDGKIS  EL   L  L    S +E+ 
Sbjct: 1   MAANNS-----NGANYLGSMDEVRKVFNKFDKNGDGKISADELKDVLRSLGSKTSPEEVK 60

Query: 61  RIMSEIDKDGDGFIDLDEFTDF--TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLK 120
           R+M EID DGDG+ID  EF+ F     ST G K+L+DAFDLYD+D+NGLISA ELH+VLK
Sbjct: 61  RVMDEIDTDGDGYIDFKEFSTFHLAGGSTDGTKELKDAFDLYDMDQNGLISANELHAVLK 120

Query: 121 RLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           +LGEK SLKDC  MI  VD DGDGHVNFEEFKKMMTRS
Sbjct: 121 KLGEKSSLKDCVNMIKKVDADGDGHVNFEEFKKMMTRS 153

[Close]

BLAST of CSPI03G21520 vs. TrEMBL
Match: A0A166AVJ5_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_010743 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 8.1e-46
Identity = 96/142 (67.61%), Postives = 111/142 (78.17%), Query Frame = 1


Query: 18  GSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLD 77
           G M+E+ +VFNKFD NGDGKI +TEL + L  L  N   DE+ RIMSEID DGDG IDL 
Sbjct: 10  GDMEEVKKVFNKFDTNGDGKIDLTELGSILRALGTNAPHDELQRIMSEIDTDGDGVIDLK 69

Query: 78  EFTDFT---SSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMIS 137
           EF+DF    S      KDL+DAFDLYD+DKNGLIS  ELH+VLKRLGEKCSLKDC +M+S
Sbjct: 70  EFSDFHLGGSDDAANLKDLRDAFDLYDLDKNGLISVSELHAVLKRLGEKCSLKDCKKMVS 129

Query: 138 SVDVDGDGHVNFEEFKKMMTRS 157
           SVDVDGDG+VNFEEFKKMM ++
Sbjct: 130 SVDVDGDGNVNFEEFKKMMNKA 151

[Close]

BLAST of CSPI03G21520 vs. TrEMBL
Match: A0A061EGG0_THECC (Calmodulin like 23, putative OS=Theobroma cacao GN=TCM_019204 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.8e-45
Identity = 97/151 (64.24%), Postives = 116/151 (76.82%), Query Frame = 1


Query: 14  SSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGF 73
           +S LGSM+E+  VFNKFDKNGDGKIS  EL + L  L    S+DE+ RIMS +DKDG+G+
Sbjct: 2   ASPLGSMEEMKEVFNKFDKNGDGKISRDELKSILSALGSPPSSDEVDRIMSMMDKDGNGY 61

Query: 74  IDLDEFTDFTSSSTGGN--------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCS 133
           +DLDEF  F  ++  GN        K+L+DAFD+YD+DKNGLISA ELH+VLKRLGEKCS
Sbjct: 62  VDLDEFIAFHGTNINGNGDDSQCGNKELKDAFDMYDLDKNGLISANELHAVLKRLGEKCS 121

Query: 134 LKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           L DC RMIS VD DGDG+VNFEEFKKMMT +
Sbjct: 122 LSDCRRMISQVDKDGDGNVNFEEFKKMMTNA 152

[Close]

BLAST of CSPI03G21520 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 167.9 bits (424), Expect = 4.9e-42
Identity = 85/144 (59.03%), Postives = 106/144 (73.61%), Query Frame = 1


Query: 15  SALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFI 74
           + LGSM++I +VF +FDKN DGKIS+ EL   +G LS N S +E   +M E D DG+GFI
Sbjct: 8   NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFI 67

Query: 75  DLDEFTDF-----TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDC 134
           DLDEF         SS+    +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC
Sbjct: 68  DLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDC 127

Query: 135 CRMISSVDVDGDGHVNFEEFKKMM 154
            RMI+ VD DGDG V+FEEFKKMM
Sbjct: 128 QRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CSPI03G21520 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 167.2 bits (422), Expect = 8.3e-42
Identity = 86/147 (58.50%), Postives = 105/147 (71.43%), Query Frame = 1


Query: 15  SALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFI 74
           S LGSMD+I +VF +FDKNGDGKISV EL   +  LS   S +E   +M + D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 75  DLDEFTDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLK 134
           DLDEF        GG         DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 135 DCCRMISSVDVDGDGHVNFEEFKKMMT 155
           DC +MIS VD+DGDG VNF+EFKKMM+
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of CSPI03G21520 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 160.2 bits (404), Expect = 1.0e-39
Identity = 78/146 (53.42%), Postives = 105/146 (71.92%), Query Frame = 1


Query: 11  TPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDG 70
           TP +  + + +E+ +VF++FD NGDGKISV EL      +  + +  E++R++ E+D D 
Sbjct: 12  TPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDR 71

Query: 71  DGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCC 130
           DG+I+LDEF+    SS+    +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC 
Sbjct: 72  DGYINLDEFSTLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCT 131

Query: 131 RMISSVDVDGDGHVNFEEFKKMMTRS 157
           RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 132 RMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CSPI03G21520 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 146.0 bits (367), Expect = 2.0e-35
Identity = 74/145 (51.03%), Postives = 103/145 (71.03%), Query Frame = 1


Query: 10  STPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKD 69
           ST  S+   +  E+ +VF+KFD NGDGKISV+EL      +  + + +E++R++ EID D
Sbjct: 8   STNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDID 67

Query: 70  GDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDC 129
            DGFI+ +EF     SS+    ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC
Sbjct: 68  CDGFINQEEFATICRSSSSA-VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDC 127

Query: 130 CRMISSVDVDGDGHVNFEEFKKMMT 155
            RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 128 VRMIGHVDTDGDGNVNFEEFQKMMS 151

[Close]

BLAST of CSPI03G21520 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 137.1 bits (344), Expect = 9.2e-33
Identity = 67/136 (49.26%), Postives = 94/136 (69.12%), Query Frame = 1


Query: 22  EIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFTD 81
           E+  VF KFD NGDGKIS  EL A +  L   +  +E+ + ++EID+ GDG+I+ +EF +
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 82  FTSSSTGGN---KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 141
             +     N   ++L+DAF +YDID NG ISA+ELH VL+ LG++CS+ +C +MI  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 142 DGDGHVNFEEFKKMMT 155
           DGDG ++FEEFK MMT
Sbjct: 157 DGDGTIDFEEFKIMMT 172

[Close]

BLAST of CSPI03G21520 vs. NCBI nr
Match: gi|449456192|ref|XP_004145834.1| (PREDICTED: probable calcium-binding protein CML23 [Cucumis sativus])

HSP 1 Score: 309.3 bits (791), Expect = 3.8e-81
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 1


Query: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60
           MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH
Sbjct: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60

Query: 61  RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 120
           RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL
Sbjct: 61  RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 120

Query: 121 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

[Close]

BLAST of CSPI03G21520 vs. NCBI nr
Match: gi|659114432|ref|XP_008457051.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo])

HSP 1 Score: 295.4 bits (755), Expect = 5.7e-77
Identity = 150/157 (95.54%), Postives = 153/157 (97.45%), Query Frame = 1


Query: 1   MAKNN-SLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEI 60
           MA NN SLSISTPGSS+LGSMDE+IRVFNKFDKNGDGKISV+EL AALGELSGNIST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

[Close]

BLAST of CSPI03G21520 vs. NCBI nr
Match: gi|567876069|ref|XP_006430624.1| (hypothetical protein CICLE_v10013012mg [Citrus clementina])

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-47
Identity = 102/158 (64.56%), Postives = 117/158 (74.05%), Query Frame = 1


Query: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60
           MA NNS      G++ LGSMDE+ +VFNKFDKNGDGKIS  EL   L  L    S +E+ 
Sbjct: 1   MAANNS-----NGANYLGSMDEVRKVFNKFDKNGDGKISADELKDVLRSLGSKTSPEEVK 60

Query: 61  RIMSEIDKDGDGFIDLDEFTDF--TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLK 120
           R+M EID DGDG+ID  EF+ F     ST G K+L+DAFDLYD+D+NGLISA ELH+VLK
Sbjct: 61  RVMDEIDTDGDGYIDFKEFSTFHLAGGSTDGTKELKDAFDLYDMDQNGLISANELHAVLK 120

Query: 121 RLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           +LGEK SLKDC  MI  VD DGDGHVNFEEFKKMMTRS
Sbjct: 121 KLGEKSSLKDCVNMIKKVDADGDGHVNFEEFKKMMTRS 153

[Close]

BLAST of CSPI03G21520 vs. NCBI nr
Match: gi|719962060|ref|XP_010262278.1| (PREDICTED: probable calcium-binding protein CML23 [Nelumbo nucifera])

HSP 1 Score: 194.1 bits (492), Expect = 1.8e-46
Identity = 100/150 (66.67%), Postives = 118/150 (78.67%), Query Frame = 1


Query: 12  PGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGD 71
           P S +L ++DE+ +VF +FD NGDGKIS TEL   LG L  + S +EIHR+MSEID DGD
Sbjct: 8   PPSVSLQNVDEVEKVFKRFDANGDGKISSTELRDVLGALGSDTSPEEIHRMMSEIDTDGD 67

Query: 72  GFIDLDEFTDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKC 131
           GFIDL EF +F  S+  GN       K+L+DAF+LYD DKNGLISAKEL++VLKRLGEKC
Sbjct: 68  GFIDLKEFAEFHKSNGTGNTKAEDELKELKDAFNLYDKDKNGLISAKELYTVLKRLGEKC 127

Query: 132 SLKDCCRMISSVDVDGDGHVNFEEFKKMMT 155
           SL+DC RMI+SVDVDGDGHVNFEEFK MMT
Sbjct: 128 SLQDCSRMINSVDVDGDGHVNFEEFKLMMT 157

[Close]

BLAST of CSPI03G21520 vs. NCBI nr
Match: gi|657972691|ref|XP_008378130.1| (PREDICTED: probable calcium-binding protein CML23 [Malus domestica])

HSP 1 Score: 191.8 bits (486), Expect = 9.0e-46
Identity = 98/156 (62.82%), Postives = 121/156 (77.56%), Query Frame = 1


Query: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGEL-SGNISTDEI 60
           M+K++ +S  + G  A GSM+E+ +VF+KFDKNGDG+IS  EL    G+L S   S +E+
Sbjct: 1   MSKSDLVSKGS-GDGAAGSMEELKKVFDKFDKNGDGRISCDELRGVFGDLGSETASLEEV 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
            RIM+E DKDGDG ID++EF +  +   G  K+L+DAFDLYD+DKNGLIS  ELH VLKR
Sbjct: 61  QRIMAEFDKDGDGHIDIEEFAEIING--GSTKELRDAFDLYDLDKNGLISTSELHEVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTR 156
           LG+KCSLKDC +MISSVDVDGDGHVNF EFKKMMTR
Sbjct: 121 LGQKCSLKDCAKMISSVDVDGDGHVNFAEFKKMMTR 153

The following BLAST results are available for this feature:

BLAST of CSPI03G21520 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML23_ARATH	8.7e-41	59.03	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=...	[more]
CML24_ARATH	1.5e-40	58.50	Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2	[more]
CML27_ARATH	1.8e-38	53.42	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=...	[more]
ALL8_OLEEU	2.9e-36	57.14	Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1	[more]
CML26_ARATH	3.5e-34	51.03	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=...	[more]

BLAST of CSPI03G21520 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCM1_CUCSA	2.7e-81	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G319280 PE=4 SV=1	[more]
A0A067F6W5_CITSI	1.5e-47	64.56	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031791mg PE=4 SV=1	[more]
V4UVY1_9ROSI	1.5e-47	64.56	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10013012mg PE=4 SV=1	[more]
A0A166AVJ5_DAUCA	8.1e-46	67.61	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_010743 PE=4 SV=1	[more]
A0A061EGG0_THECC	1.8e-45	64.24	Calmodulin like 23, putative OS=Theobroma cacao GN=TCM_019204 PE=4 SV=1	[more]

BLAST of CSPI03G21520 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G66400.1	4.9e-42	59.03	calmodulin like 23	[more]
AT5G37770.1	8.3e-42	58.50	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.0e-39	53.42	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	2.0e-35	51.03	EF hand calcium-binding protein family	[more]
AT1G24620.1	9.2e-33	49.26	EF hand calcium-binding protein family	[more]

BLAST of CSPI03G21520 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449456192\|ref\|XP_004145834.1\|	3.8e-81	100.00	PREDICTED: probable calcium-binding protein CML23 [Cucumis sativus]	[more]
gi\|659114432\|ref\|XP_008457051.1\|	5.7e-77	95.54	PREDICTED: probable calcium-binding protein CML27 [Cucumis melo]	[more]
gi\|567876069\|ref\|XP_006430624.1\|	2.1e-47	64.56	hypothetical protein CICLE_v10013012mg [Citrus clementina]	[more]
gi\|719962060\|ref\|XP_010262278.1\|	1.8e-46	66.67	PREDICTED: probable calcium-binding protein CML23 [Nelumbo nucifera]	[more]
gi\|657972691\|ref\|XP_008378130.1\|	9.0e-46	62.82	PREDICTED: probable calcium-binding protein CML23 [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G21520.1	CSPI03G21520.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 22..83 score: 5.6E-14coord: 92..153 score: 1.2
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 128..156 score: 2.1E-4coord: 22..50 score: 2.4E-5coord: 58..86 score: 2.1E-5coord: 92..120 score: 3.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 124..156 score: 13.174coord: 18..53 score: 14.569coord: 88..123 score: 14.318coord: 54..82 score: 9
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 20..154 score: 3.0
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 20..155 score: 3.55
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113 score: -coord: 137..149 score: -coord: 31..43 score: -coord: 67..79 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 7..156 score: 1.9
None	No IPR available	PANTHER	PTHR10891:SF573	CALCIUM-BINDING PROTEIN CML24-RELATED	coord: 7..156 score: 1.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G21520	Cla013188	Watermelon (97103) v1	cpiwmB245
CSPI03G21520	Csa3G319280	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G21520	MELO3C020263	Melon (DHL92) v3.5.1	cpimeB243
CSPI03G21520	ClCG05G011200	Watermelon (Charleston Gray)	cpiwcgB240
CSPI03G21520	Lsi05G003280	Bottle gourd (USVL1VR-Ls)	cpilsiB210
CSPI03G21520	MELO3C020263.2	Melon (DHL92) v3.6.1	cpimedB240
CSPI03G21520	CsaV3_3G021210	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G21520	Cla97C05G093380	Watermelon (97103) v2	cpiwmbB224
CSPI03G21520	Bhi01G001514	Wax gourd	cpiwgoB271
CSPI03G21520	Cucsa.375430	Cucumber (Gy14) v1	cgycpiB554
CSPI03G21520	CmaCh01G003620	Cucurbita maxima (Rimu)	cmacpiB458
CSPI03G21520	CmaCh14G008380	Cucurbita maxima (Rimu)	cmacpiB244
CSPI03G21520	CmoCh14G008530	Cucurbita moschata (Rifu)	cmocpiB232
CSPI03G21520	CmoCh01G003790	Cucurbita moschata (Rifu)	cmocpiB446
CSPI03G21520	Cp4.1LG03g05830	Cucurbita pepo (Zucchini)	cpecpiB602
CSPI03G21520	Cp4.1LG02g16750	Cucurbita pepo (Zucchini)	cpecpiB552
CSPI03G21520	CsGy3G020790	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G21520	Carg07449	Silver-seed gourd	carcpiB0798
CSPI03G21520	Carg26921	Silver-seed gourd	carcpiB0545

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None