Cucsa.375430 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.375430
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionEF hand calcium-binding protein family
Locationscaffold03746 : 518464 .. 518877 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAG

mRNA sequence

ATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAG

Coding sequence (CDS)

ATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAG

Protein sequence

MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS*
Homology
[Close]
BLAST of Cucsa.375430 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.1e-39
Identity = 82/139 (58.99%), Postives = 102/139 (73.38%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           M++I +VF +FDKN DGKIS+ EL   +G LS N S +E   +M E D DG+GFIDLDEF
Sbjct: 13  MEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEF 72

Query: 61  TDF-----TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMIS 120
                    SS+    +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC RMI+
Sbjct: 73  VALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMIN 132

Query: 121 SVDVDGDGHVNFEEFKKMM 135
            VD DGDG V+FEEFKKMM
Sbjct: 133 KVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cucsa.375430 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 4.2e-39
Identity = 82/142 (57.75%), Postives = 101/142 (71.13%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           MD+I +VF +FDKNGDGKISV EL   +  LS   S +E   +M + D DG+GFIDLDEF
Sbjct: 15  MDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEF 74

Query: 61  TDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM 120
                   GG         DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++DC +M
Sbjct: 75  VALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKM 134

Query: 121 ISSVDVDGDGHVNFEEFKKMMT 136
           IS VD+DGDG VNF+EFKKMM+
Sbjct: 135 ISKVDIDGDGCVNFDEFKKMMS 156

[Close]
BLAST of Cucsa.375430 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 7.9e-38
Identity = 76/136 (55.88%), Postives = 100/136 (73.53%), Query Frame = 1 

Query: 2   DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 61
           +E+ +VF++FD NGDGKISV EL      +  + +  E++R++ E+D D DG+I+LDEF+
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 62  DFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDG 121
               SS+    +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI  VD DG
Sbjct: 82  TLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDADG 141

Query: 122 DGHVNFEEFKKMMTRS 138
           DG+VNFEEF+KMMT S
Sbjct: 142 DGNVNFEEFQKMMTSS 156

[Close]
BLAST of Cucsa.375430 vs. Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 8.7e-37
Identity = 80/140 (57.14%), Postives = 98/140 (70.00%), Query Frame = 1 

Query: 2   DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 61
           +E+  VFN+FD NGDGKIS  ELA  L  L  N S +EI RIM EID D DGFI++ EF 
Sbjct: 19  NEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFA 78

Query: 62  DFTSS------STGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMIS 121
            F  +      S+GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ +  DC  MI 
Sbjct: 79  AFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIK 138

Query: 122 SVDVDGDGHVNFEEFKKMMT 136
           SVD DGDG+V+FEEFKKMMT
Sbjct: 139 SVDSDGDGYVSFEEFKKMMT 158

[Close]
BLAST of Cucsa.375430 vs. Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.4e-34
Identity = 71/133 (53.38%), Postives = 98/133 (73.68%), Query Frame = 1 

Query: 3   EIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFTD 62
           E+ +VF+KFD NGDGKISV+EL      +  + + +E++R++ EID D DGFI+ +EF  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 63  FTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGD 122
              SS+    ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC RMI  VD DGD
Sbjct: 80  ICRSSSSA-VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDTDGD 139

Query: 123 GHVNFEEFKKMMT 136
           G+VNFEEF+KMM+
Sbjct: 140 GNVNFEEFQKMMS 151


HSP 2 Score: 48.1 bits (113), Expect = 8.8e-05
Identity = 22/66 (33.33%), Postives = 40/66 (60.61%), Query Frame = 1 

Query: 65  SSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGH 124
           S++   + +L+  FD +D + +G IS  EL +V K +G   + ++  R++  +D+D DG 
Sbjct: 12  STTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGF 71

Query: 125 VNFEEF 131
           +N EEF
Sbjct: 72  INQEEF 77

[Close]
BLAST of Cucsa.375430 vs. TrEMBL
Match: A0A0A0LCM1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G319280 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.3e-71
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF
Sbjct: 20  MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 79

Query: 61  TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD 120
           TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD
Sbjct: 80  TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD 139

Query: 121 GDGHVNFEEFKKMMTRS 138
           GDGHVNFEEFKKMMTRS
Sbjct: 140 GDGHVNFEEFKKMMTRS 156

[Close]
BLAST of Cucsa.375430 vs. TrEMBL
Match: V7B7N2_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_008G235100g PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 9.4e-46
Identity = 92/138 (66.67%), Postives = 110/138 (79.71%), Query Frame = 1 

Query: 2   DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 61
           DE+ ++FNKFDKNGDGKIS+TEL   LG L    + +E+ RIM E+D++GDG+IDL EF 
Sbjct: 3   DEVRQIFNKFDKNGDGKISITELRDMLGTLGSKTTKEELKRIMEELDQNGDGYIDLKEFA 62

Query: 62  DFTSSSTGGN--KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 121
           DF  +  G +  K+L+DAFDLYD+DKNGLISAKELH VL +LGEKCSL DC RMIS+VD 
Sbjct: 63  DFHCNDAGNDESKELRDAFDLYDVDKNGLISAKELHDVLSKLGEKCSLSDCRRMISNVDA 122

Query: 122 DGDGHVNFEEFKKMMTRS 138
           DGDG VNFEEFKKMM RS
Sbjct: 123 DGDGSVNFEEFKKMMARS 140

[Close]
BLAST of Cucsa.375430 vs. TrEMBL
Match: A0A166AVJ5_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_010743 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.2e-45
Identity = 95/140 (67.86%), Postives = 110/140 (78.57%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           M+E+ +VFNKFD NGDGKI +TEL + L  L  N   DE+ RIMSEID DGDG IDL EF
Sbjct: 12  MEEVKKVFNKFDTNGDGKIDLTELGSILRALGTNAPHDELQRIMSEIDTDGDGVIDLKEF 71

Query: 61  TDFT---SSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSV 120
           +DF    S      KDL+DAFDLYD+DKNGLIS  ELH+VLKRLGEKCSLKDC +M+SSV
Sbjct: 72  SDFHLGGSDDAANLKDLRDAFDLYDLDKNGLISVSELHAVLKRLGEKCSLKDCKKMVSSV 131

Query: 121 DVDGDGHVNFEEFKKMMTRS 138
           DVDGDG+VNFEEFKKMM ++
Sbjct: 132 DVDGDGNVNFEEFKKMMNKA 151

[Close]
BLAST of Cucsa.375430 vs. TrEMBL
Match: A0A0L9UWX6_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan07g073800 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 3.6e-45
Identity = 91/138 (65.94%), Postives = 110/138 (79.71%), Query Frame = 1 

Query: 2   DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 61
           DE+ ++FNKFDKNGDGKIS+TEL   LG L    + +E+ R+M E+DK+GDG+IDL EF 
Sbjct: 3   DEVRQIFNKFDKNGDGKISITELRDMLGTLGSKTTKEELKRMMEELDKNGDGYIDLKEFA 62

Query: 62  DFTSSSTGGN--KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 121
           DF  +  G +  K+L+DAFDLYD+DKNGLI+AKELH VL +LGEKCSL DC RMIS+VD 
Sbjct: 63  DFHCNDAGNDDSKELRDAFDLYDVDKNGLITAKELHDVLCKLGEKCSLSDCRRMISNVDA 122

Query: 122 DGDGHVNFEEFKKMMTRS 138
           DGDG VNFEEFKKMM RS
Sbjct: 123 DGDGSVNFEEFKKMMARS 140

[Close]
BLAST of Cucsa.375430 vs. TrEMBL
Match: V4UVY1_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10013012mg PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 3.6e-45
Identity = 93/139 (66.91%), Postives = 106/139 (76.26%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           MDE+ +VFNKFDKNGDGKIS  EL   L  L    S +E+ R+M EID DGDG+ID  EF
Sbjct: 15  MDEVRKVFNKFDKNGDGKISADELKDVLRSLGSKTSPEEVKRVMDEIDTDGDGYIDFKEF 74

Query: 61  TDF--TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVD 120
           + F     ST G K+L+DAFDLYD+D+NGLISA ELH+VLK+LGEK SLKDC  MI  VD
Sbjct: 75  STFHLAGGSTDGTKELKDAFDLYDMDQNGLISANELHAVLKKLGEKSSLKDCVNMIKKVD 134

Query: 121 VDGDGHVNFEEFKKMMTRS 138
            DGDGHVNFEEFKKMMTRS
Sbjct: 135 ADGDGHVNFEEFKKMMTRS 153

[Close]
BLAST of Cucsa.375430 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 164.1 bits (414), Expect = 6.2e-41
Identity = 82/139 (58.99%), Postives = 102/139 (73.38%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           M++I +VF +FDKN DGKIS+ EL   +G LS N S +E   +M E D DG+GFIDLDEF
Sbjct: 13  MEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEF 72

Query: 61  TDF-----TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMIS 120
                    SS+    +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC RMI+
Sbjct: 73  VALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMIN 132

Query: 121 SVDVDGDGHVNFEEFKKMM 135
            VD DGDG V+FEEFKKMM
Sbjct: 133 KVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cucsa.375430 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 162.2 bits (409), Expect = 2.4e-40
Identity = 82/142 (57.75%), Postives = 101/142 (71.13%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           MD+I +VF +FDKNGDGKISV EL   +  LS   S +E   +M + D DG+GFIDLDEF
Sbjct: 15  MDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEF 74

Query: 61  TDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM 120
                   GG         DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++DC +M
Sbjct: 75  VALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKM 134

Query: 121 ISSVDVDGDGHVNFEEFKKMMT 136
           IS VD+DGDG VNF+EFKKMM+
Sbjct: 135 ISKVDIDGDGCVNFDEFKKMMS 156

[Close]
BLAST of Cucsa.375430 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 157.9 bits (398), Expect = 4.4e-39
Identity = 76/136 (55.88%), Postives = 100/136 (73.53%), Query Frame = 1 

Query: 2   DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 61
           +E+ +VF++FD NGDGKISV EL      +  + +  E++R++ E+D D DG+I+LDEF+
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 62  DFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDG 121
               SS+    +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI  VD DG
Sbjct: 82  TLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDADG 141

Query: 122 DGHVNFEEFKKMMTRS 138
           DG+VNFEEF+KMMT S
Sbjct: 142 DGNVNFEEFQKMMTSS 156

[Close]
BLAST of Cucsa.375430 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 147.1 bits (370), Expect = 7.8e-36
Identity = 71/133 (53.38%), Postives = 98/133 (73.68%), Query Frame = 1 

Query: 3   EIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFTD 62
           E+ +VF+KFD NGDGKISV+EL      +  + + +E++R++ EID D DGFI+ +EF  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 63  FTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGD 122
              SS+    ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC RMI  VD DGD
Sbjct: 80  ICRSSSSA-VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDTDGD 139

Query: 123 GHVNFEEFKKMMT 136
           G+VNFEEF+KMM+
Sbjct: 140 GNVNFEEFQKMMS 151


HSP 2 Score: 48.1 bits (113), Expect = 5.0e-06
Identity = 22/66 (33.33%), Postives = 40/66 (60.61%), Query Frame = 1 

Query: 65  SSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGH 124
           S++   + +L+  FD +D + +G IS  EL +V K +G   + ++  R++  +D+D DG 
Sbjct: 12  STTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGF 71

Query: 125 VNFEEF 131
           +N EEF
Sbjct: 72  INQEEF 77

[Close]
BLAST of Cucsa.375430 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 138.7 bits (348), Expect = 2.8e-33
Identity = 67/136 (49.26%), Postives = 94/136 (69.12%), Query Frame = 1 

Query: 3   EIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFTD 62
           E+  VF KFD NGDGKIS  EL A +  L   +  +E+ + ++EID+ GDG+I+ +EF +
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 63  FTSSSTGGN---KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 122
             +     N   ++L+DAF +YDID NG ISA+ELH VL+ LG++CS+ +C +MI  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 123 DGDGHVNFEEFKKMMT 136
           DGDG ++FEEFK MMT
Sbjct: 157 DGDGTIDFEEFKIMMT 172

[Close]
BLAST of Cucsa.375430 vs. NCBI nr
Match: gi|449456192|ref|XP_004145834.1| (PREDICTED: probable calcium-binding protein CML23 [Cucumis sativus])

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-71
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF
Sbjct: 20  MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 79

Query: 61  TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD 120
           TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD
Sbjct: 80  TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD 139

Query: 121 GDGHVNFEEFKKMMTRS 138
           GDGHVNFEEFKKMMTRS
Sbjct: 140 GDGHVNFEEFKKMMTRS 156

[Close]
BLAST of Cucsa.375430 vs. NCBI nr
Match: gi|659114432|ref|XP_008457051.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo])

HSP 1 Score: 270.8 bits (691), Expect = 1.3e-69
Identity = 133/137 (97.08%), Postives = 135/137 (98.54%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           MDE+IRVFNKFDKNGDGKISV+EL AALGELSGNIST EIHRIMSEIDKDGDGFIDLDEF
Sbjct: 21  MDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEIHRIMSEIDKDGDGFIDLDEF 80

Query: 61  TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD 120
           TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD
Sbjct: 81  TDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVD 140

Query: 121 GDGHVNFEEFKKMMTRS 138
           GDGHVNFEEFKKMMTRS
Sbjct: 141 GDGHVNFEEFKKMMTRS 157

[Close]
BLAST of Cucsa.375430 vs. NCBI nr
Match: gi|719962060|ref|XP_010262278.1| (PREDICTED: probable calcium-binding protein CML23 [Nelumbo nucifera])

HSP 1 Score: 191.8 bits (486), Expect = 7.9e-46
Identity = 97/142 (68.31%), Postives = 113/142 (79.58%), Query Frame = 1 

Query: 1   MDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEF 60
           +DE+ +VF +FD NGDGKIS TEL   LG L  + S +EIHR+MSEID DGDGFIDL EF
Sbjct: 16  VDEVEKVFKRFDANGDGKISSTELRDVLGALGSDTSPEEIHRMMSEIDTDGDGFIDLKEF 75

Query: 61  TDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM 120
            +F  S+  GN       K+L+DAF+LYD DKNGLISAKEL++VLKRLGEKCSL+DC RM
Sbjct: 76  AEFHKSNGTGNTKAEDELKELKDAFNLYDKDKNGLISAKELYTVLKRLGEKCSLQDCSRM 135

Query: 121 ISSVDVDGDGHVNFEEFKKMMT 136
           I+SVDVDGDGHVNFEEFK MMT
Sbjct: 136 INSVDVDGDGHVNFEEFKLMMT 157

[Close]
BLAST of Cucsa.375430 vs. NCBI nr
Match: gi|950987490|ref|XP_014503786.1| (PREDICTED: probable calcium-binding protein CML23 [Vigna radiata var. radiata])

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-45
Identity = 91/138 (65.94%), Postives = 111/138 (80.43%), Query Frame = 1 

Query: 2   DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 61
           DE+ ++FNKFDKNGDGKIS+TEL   LG L    + +E+ R+M E+DK+GDG+IDL EF 
Sbjct: 3   DEVRQIFNKFDKNGDGKISITELRDMLGTLGSKTTKEELKRMMEELDKNGDGYIDLKEFA 62

Query: 62  DFTSSSTGGN--KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 121
           DF  +  G +  K+L+DAFDLYD+DKNGLI+AKELH VL++LGEKCSL DC RMIS+VD 
Sbjct: 63  DFHCNDAGNDDSKELRDAFDLYDVDKNGLITAKELHDVLRKLGEKCSLSDCRRMISNVDA 122

Query: 122 DGDGHVNFEEFKKMMTRS 138
           DGDG VNFEEFKKMM RS
Sbjct: 123 DGDGSVNFEEFKKMMARS 140

[Close]
BLAST of Cucsa.375430 vs. NCBI nr
Match: gi|593490061|ref|XP_007141898.1| (hypothetical protein PHAVU_008G235100g [Phaseolus vulgaris])

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-45
Identity = 92/138 (66.67%), Postives = 110/138 (79.71%), Query Frame = 1 

Query: 2   DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 61
           DE+ ++FNKFDKNGDGKIS+TEL   LG L    + +E+ RIM E+D++GDG+IDL EF 
Sbjct: 3   DEVRQIFNKFDKNGDGKISITELRDMLGTLGSKTTKEELKRIMEELDQNGDGYIDLKEFA 62

Query: 62  DFTSSSTGGN--KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDV 121
           DF  +  G +  K+L+DAFDLYD+DKNGLISAKELH VL +LGEKCSL DC RMIS+VD 
Sbjct: 63  DFHCNDAGNDESKELRDAFDLYDVDKNGLISAKELHDVLSKLGEKCSLSDCRRMISNVDA 122

Query: 122 DGDGHVNFEEFKKMMTRS 138
           DGDG VNFEEFKKMM RS
Sbjct: 123 DGDGSVNFEEFKKMMARS 140

The following BLAST results are available for this feature:
BLAST of Cucsa.375430 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML23_ARATH1.1e-3958.99Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML24_ARATH4.2e-3957.75Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2[more]
CML27_ARATH7.9e-3855.88Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=... [more]
ALL8_OLEEU8.7e-3757.14Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1[more]
CML26_ARATH1.4e-3453.38Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=... [more]
BLAST of Cucsa.375430 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LCM1_CUCSA1.3e-71100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G319280 PE=4 SV=1[more]
V7B7N2_PHAVU9.4e-4666.67Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_008G235100g PE=4 SV=1[more]
A0A166AVJ5_DAUCA1.2e-4567.86Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_010743 PE=4 SV=1[more]
A0A0L9UWX6_PHAAN3.6e-4565.94Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan07g073800 PE=4 SV=1[more]
V4UVY1_9ROSI3.6e-4566.91Uncharacterized protein OS=Citrus clementina GN=CICLE_v10013012mg PE=4 SV=1[more]
BLAST of Cucsa.375430 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G66400.16.2e-4158.99 calmodulin like 23[more]
AT5G37770.12.4e-4057.75 EF hand calcium-binding protein family[more]
AT1G18210.14.4e-3955.88 Calcium-binding EF-hand family protein[more]
AT1G73630.17.8e-3653.38 EF hand calcium-binding protein family[more]
AT1G24620.12.8e-3349.26 EF hand calcium-binding protein family[more]
BLAST of Cucsa.375430 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449456192|ref|XP_004145834.1|1.9e-71100.00PREDICTED: probable calcium-binding protein CML23 [Cucumis sativus][more]
gi|659114432|ref|XP_008457051.1|1.3e-6997.08PREDICTED: probable calcium-binding protein CML27 [Cucumis melo][more]
gi|719962060|ref|XP_010262278.1|7.9e-4668.31PREDICTED: probable calcium-binding protein CML23 [Nelumbo nucifera][more]
gi|950987490|ref|XP_014503786.1|1.3e-4565.94PREDICTED: probable calcium-binding protein CML23 [Vigna radiata var. radiata][more]
gi|593490061|ref|XP_007141898.1|1.3e-4566.67hypothetical protein PHAVU_008G235100g [Phaseolus vulgaris][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.375430.1Cucsa.375430.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 72..134
score: 8.8E-12coord: 3..64
score: 4.1
IPR002048EF-hand domainSMARTSM00054efh_1coord: 39..67
score: 2.1E-5coord: 109..137
score: 2.1E-4coord: 73..101
score: 3.2E-5coord: 3..31
score: 2.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 69..104
score: 14.318coord: 105..137
score: 13.174coord: 35..63
score: 9.966coord: 1..34
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 2..135
score: 7.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 2..136
score: 3.46
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 12..24
score: -coord: 82..94
score: -coord: 48..60
score: -coord: 118..130
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 1..137
score: 1.2
NoneNo IPR availablePANTHERPTHR10891:SF605CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 1..137
score: 1.2