Carg20175 (gene) Silver-seed gourd

NameCarg20175
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCalcium-binding protein
LocationCucurbita_argyrosperma_scaffold_154 : 43825 .. 44250 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATCACCAGTGCGCGGCACTACGACCGCGTCTTGCGTTACTTCGATGAAGATGGCGACGGCAAAATCTCGCCGTCGGAGCTGAGGAATCGTCTCGGATTGATCGGCGGAGAGCTGCTGCCGGCGGAGGCAGAGGTGGCGGTGGAATCGCTCGACTCCGACGGCGACGGACTGCTCTGCGTCGGTGATATCGAGCGGCTACTGGACGTGGGCGAGGAAGAGAAATTGAAGGACTTGAGAGAGGCTTTCGGATTGTACGATGCCGAAGGCTGCGGGTTCATTACTCCGAAAAGCTTGAAGAGAATGCTTAAAAAATTGGGGGAAACGAAATCCACGGCGGAGTGTAAAGTGATGATTCGCCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCCTGA

mRNA sequence

ATGGAGATCACCAGTGCGCGGCACTACGACCGCGTCTTGCGTTACTTCGATGAAGATGGCGACGGCAAAATCTCGCCGTCGGAGCTGAGGAATCGTCTCGGATTGATCGGCGGAGAGCTGCTGCCGGCGGAGGCAGAGGTGGCGGTGGAATCGCTCGACTCCGACGGCGACGGACTGCTCTGCGTCGGTGATATCGAGCGGCTACTGGACGTGGGCGAGGAAGAGAAATTGAAGGACTTGAGAGAGGCTTTCGGATTGTACGATGCCGAAGGCTGCGGGTTCATTACTCCGAAAAGCTTGAAGAGAATGCTTAAAAAATTGGGGGAAACGAAATCCACGGCGGAGTGTAAAGTGATGATTCGCCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCCTGA

Coding sequence (CDS)

ATGGAGATCACCAGTGCGCGGCACTACGACCGCGTCTTGCGTTACTTCGATGAAGATGGCGACGGCAAAATCTCGCCGTCGGAGCTGAGGAATCGTCTCGGATTGATCGGCGGAGAGCTGCTGCCGGCGGAGGCAGAGGTGGCGGTGGAATCGCTCGACTCCGACGGCGACGGACTGCTCTGCGTCGGTGATATCGAGCGGCTACTGGACGTGGGCGAGGAAGAGAAATTGAAGGACTTGAGAGAGGCTTTCGGATTGTACGATGCCGAAGGCTGCGGGTTCATTACTCCGAAAAGCTTGAAGAGAATGCTTAAAAAATTGGGGGAAACGAAATCCACGGCGGAGTGTAAAGTGATGATTCGCCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCCTGA

Protein sequence

MEITSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGDIERLLDVGEEEKLKDLREAFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRFDLNGDGLISFEEFQIMMA
BLAST of Carg20175 vs. NCBI nr
Match: XP_022925940.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 238.8 bits (608), Expect = 1.1e-59
Identity = 139/141 (98.58%), Postives = 139/141 (98.58%), Query Frame = 0

Query: 1   MEITSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLL 60
           MEITSA HYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAE AVESLDSDGDGLL
Sbjct: 1   MEITSAAHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEAAVESLDSDGDGLL 60

Query: 61  CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI 120
           CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI
Sbjct: 61  CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI 120

Query: 121 RRFDLNGDGLISFEEFQIMMA 142
           RRFDLNGDGLISFEEFQIMMA
Sbjct: 121 RRFDLNGDGLISFEEFQIMMA 141

BLAST of Carg20175 vs. NCBI nr
Match: XP_022966390.1 (probable calcium-binding protein CML31 [Cucurbita maxima])

HSP 1 Score: 237.7 bits (605), Expect = 2.5e-59
Identity = 138/141 (97.87%), Postives = 139/141 (98.58%), Query Frame = 0

Query: 1   MEITSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLL 60
           MEITSA HYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAE AVESLDSDGDGLL
Sbjct: 1   MEITSAAHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEAAVESLDSDGDGLL 60

Query: 61  CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI 120
           CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI
Sbjct: 61  CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI 120

Query: 121 RRFDLNGDGLISFEEFQIMMA 142
           RRFDLNGDGLISF+EFQIMMA
Sbjct: 121 RRFDLNGDGLISFDEFQIMMA 141

BLAST of Carg20175 vs. NCBI nr
Match: XP_023529455.1 (probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 236.9 bits (603), Expect = 4.2e-59
Identity = 138/141 (97.87%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 1   MEITSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLL 60
           MEITSA HYD VLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAE AVESLDSDGDGLL
Sbjct: 1   MEITSAAHYDHVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEAAVESLDSDGDGLL 60

Query: 61  CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI 120
           CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI
Sbjct: 61  CVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI 120

Query: 121 RRFDLNGDGLISFEEFQIMMA 142
           RRFDLNGDGLISFEEFQIMMA
Sbjct: 121 RRFDLNGDGLISFEEFQIMMA 141

BLAST of Carg20175 vs. NCBI nr
Match: XP_008464450.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo])

HSP 1 Score: 208.4 bits (529), Expect = 1.6e-50
Identity = 107/142 (75.35%), Postives = 112/142 (78.87%), Query Frame = 0

Query: 1   MEIT-SARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGL 60
           MEI  S   YDRVL YFDEDGDGKISPSELRNRLGLIGGELL AEAE AVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 61  LCVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVM 120
           LC GDI                  F LYD+EGCGFITPK+LK+ML+KLGE KST ECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 121 IRRFDLNGDGLISFEEFQIMMA 142
           IRRFDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

BLAST of Carg20175 vs. NCBI nr
Match: XP_004138054.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis sativus] >KGN63491.1 hypothetical protein Csa_1G002110 [Cucumis sativus])

HSP 1 Score: 207.6 bits (527), Expect = 2.8e-50
Identity = 109/139 (78.42%), Postives = 115/139 (82.73%), Query Frame = 0

Query: 3   ITSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCV 62
           + S   YDRVL YFDEDGDGKISPSELRNRLGLIGGEL  AEAE AVESLDSDGDGLLCV
Sbjct: 4   VKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLDSDGDGLLCV 63

Query: 63  GDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRR 122
           GDI      XXXXX       F LYD+EGCGFITPK+LK+ML+KLGE KST ECK+MIRR
Sbjct: 64  GDIERLLEVXXXXXLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRR 123

Query: 123 FDLNGDGLISFEEFQIMMA 142
           FDLNGDGLISFEEFQIMMA
Sbjct: 124 FDLNGDGLISFEEFQIMMA 142

BLAST of Carg20175 vs. TAIR10
Match: AT1G76650.1 (calmodulin-like 38)

HSP 1 Score: 105.5 bits (262), Expect = 2.7e-23
Identity = 59/135 (43.70%), Postives = 78/135 (57.78%), Query Frame = 0

Query: 7   RHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGDI- 66
           R  + V  Y D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   +  
Sbjct: 42  RELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFS 101

Query: 67  XXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRFDL 126
                             F LY AEG   ITP+SLK MLKKLGE+++T +C+VMI  FDL
Sbjct: 102 QLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDL 161

Query: 127 NGDGLISFEEFQIMM 141
           N DG++SF+EF +MM
Sbjct: 162 NADGVLSFDEFALMM 176

BLAST of Carg20175 vs. TAIR10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 102.8 bits (255), Expect = 1.7e-22
Identity = 57/138 (41.30%), Postives = 79/138 (57.25%), Query Frame = 0

Query: 7   RHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGD-- 66
           R  + V  Y D + DG+IS  EL+     +G ++   EAE AV+  D DGDG+L + +  
Sbjct: 21  RDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFA 80

Query: 67  --IXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRR 126
             I                  F +Y A+G   ITP SLK ML KLGE+++T +CKVMI+ 
Sbjct: 81  LLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQA 140

Query: 127 FDLNGDGLISFEEFQIMM 141
           FDLN DG++SF+EF +MM
Sbjct: 141 FDLNADGVLSFDEFALMM 158

BLAST of Carg20175 vs. TAIR10
Match: AT5G42380.1 (calmodulin like 37)

HSP 1 Score: 94.4 bits (233), Expect = 6.2e-20
Identity = 54/132 (40.91%), Postives = 73/132 (55.30%), Query Frame = 0

Query: 12  VLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGD---IXXX 71
           V  Y D + DGKIS  EL++ + L+GG L   E E  V++ D DGDG +   +   +   
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 72  XXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRFDLNGD 131
                          FG+Y  EG  FIT  SL+R L +LGE+ +   CKVMIR FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 132 GLISFEEFQIMM 141
           G++SF+EF +MM
Sbjct: 173 GVLSFDEFVLMM 184

BLAST of Carg20175 vs. TAIR10
Match: AT3G50770.1 (calmodulin-like 41)

HSP 1 Score: 90.5 bits (223), Expect = 8.9e-19
Identity = 50/137 (36.50%), Postives = 76/137 (55.47%), Query Frame = 0

Query: 11  RVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGDI----- 70
           +V  +FD DGDGKIS  ELR+  G +G  +    A+ A+  +D+D DG L   D      
Sbjct: 67  QVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMT 126

Query: 71  -XXXXXXXXXXXXXXXXXXFGLYDAE-GCGFITPKSLKRMLKKLGETKSTAECKVMIRRF 130
                              F +++ E G G ITPK L++ML KLGE+++  EC+ MI+ +
Sbjct: 127 RRDLYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFY 186

Query: 131 DLNGDGLISFEEFQIMM 141
           D++G+G++ F EF+ MM
Sbjct: 187 DIDGNGILDFHEFRQMM 203

BLAST of Carg20175 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 84.0 bits (206), Expect = 8.3e-17
Identity = 50/139 (35.97%), Postives = 70/139 (50.36%), Query Frame = 0

Query: 4   TSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVG 63
           T  R  + V + FD +GDGKIS  EL   +  +G E+   E E A+  +D  GDG +   
Sbjct: 33  TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFE 92

Query: 64  DI--XXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIR 123
           +                     F +YD +G G I+ + L  +L+ LG+  S AEC+ MI 
Sbjct: 93  EFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 152

Query: 124 RFDLNGDGLISFEEFQIMM 141
             D +GDG I FEEF+IMM
Sbjct: 153 GVDKDGDGTIDFEEFKIMM 171

BLAST of Carg20175 vs. Swiss-Prot
Match: sp|Q9SRE6|CML38_ARATH (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 4.8e-22
Identity = 59/135 (43.70%), Postives = 78/135 (57.78%), Query Frame = 0

Query: 7   RHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGDI- 66
           R  + V  Y D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   +  
Sbjct: 42  RELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFS 101

Query: 67  XXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRFDL 126
                             F LY AEG   ITP+SLK MLKKLGE+++T +C+VMI  FDL
Sbjct: 102 QLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDL 161

Query: 127 NGDGLISFEEFQIMM 141
           N DG++SF+EF +MM
Sbjct: 162 NADGVLSFDEFALMM 176

BLAST of Carg20175 vs. Swiss-Prot
Match: sp|Q8RYJ8|CML19_ORYSJ (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-21
Identity = 62/143 (43.36%), Postives = 79/143 (55.24%), Query Frame = 0

Query: 3   ITSARHYDRVLRYFDEDGDGKISPSELRNRL-GLIGGELLPAEAEVAVESLDSDGDGLLC 62
           + +   + RV   FD D DGKIS +ELR  +   +G ++   EAE  V S D+D DGLL 
Sbjct: 2   VAATAEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLD 61

Query: 63  VGDI----XXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECK 122
             +                       F +Y+ EG G ITP SLKRML KLG  +   EC+
Sbjct: 62  EEEFTKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQ 121

Query: 123 VMIRRFDLNGDGLISFEEFQIMM 141
            MI RFDL+GDG+ISFEEF+IMM
Sbjct: 122 TMICRFDLDGDGVISFEEFKIMM 144

BLAST of Carg20175 vs. Swiss-Prot
Match: sp|Q9SRE7|CML39_ARATH (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 3.1e-21
Identity = 57/138 (41.30%), Postives = 79/138 (57.25%), Query Frame = 0

Query: 7   RHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGD-- 66
           R  + V  Y D + DG+IS  EL+     +G ++   EAE AV+  D DGDG+L + +  
Sbjct: 21  RDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFA 80

Query: 67  --IXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRR 126
             I                  F +Y A+G   ITP SLK ML KLGE+++T +CKVMI+ 
Sbjct: 81  LLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQA 140

Query: 127 FDLNGDGLISFEEFQIMM 141
           FDLN DG++SF+EF +MM
Sbjct: 141 FDLNADGVLSFDEFALMM 158

BLAST of Carg20175 vs. Swiss-Prot
Match: sp|Q8RYJ9|CML23_ORYSJ (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.2e-19
Identity = 72/149 (48.32%), Postives = 93/149 (62.42%), Query Frame = 0

Query: 3   ITSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESL-DSDGDGLLC 62
           + ++  + RV   FD+DGDGKIS +ELR  +    GE +P E   A+ +L D+DGDGLL 
Sbjct: 1   MVASDEFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLD 60

Query: 63  ----------VGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETK 122
                     +   XXXXXXXXXXXX      F +Y+ EG G ITP SLK ML KLG   
Sbjct: 61  EEEFVRLVTEMXXXXXXXXXXXXXXXRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHL 120

Query: 123 STAECKVMIRRFDLNGDGLISFEEFQIMM 141
             AEC+ MI RFD+NGDG+++F+EF+ MM
Sbjct: 121 DVAECQAMICRFDMNGDGVLTFDEFKTMM 149

BLAST of Carg20175 vs. Swiss-Prot
Match: sp|Q9FIH9|CML37_ARATH (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.1e-18
Identity = 54/132 (40.91%), Postives = 73/132 (55.30%), Query Frame = 0

Query: 12  VLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGD---IXXX 71
           V  Y D + DGKIS  EL++ + L+GG L   E E  V++ D DGDG +   +   +   
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 72  XXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRFDLNGD 131
                          FG+Y  EG  FIT  SL+R L +LGE+ +   CKVMIR FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 132 GLISFEEFQIMM 141
           G++SF+EF +MM
Sbjct: 173 GVLSFDEFVLMM 184

BLAST of Carg20175 vs. TrEMBL
Match: tr|A0A1S3CN12|A0A1S3CN12_CUCME (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-50
Identity = 107/142 (75.35%), Postives = 112/142 (78.87%), Query Frame = 0

Query: 1   MEIT-SARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGL 60
           MEI  S   YDRVL YFDEDGDGKISPSELRNRLGLIGGELL AEAE AVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 61  LCVGDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVM 120
           LC GDI                  F LYD+EGCGFITPK+LK+ML+KLGE KST ECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 121 IRRFDLNGDGLISFEEFQIMMA 142
           IRRFDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

BLAST of Carg20175 vs. TrEMBL
Match: tr|A0A0A0LU40|A0A0A0LU40_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.8e-50
Identity = 109/139 (78.42%), Postives = 115/139 (82.73%), Query Frame = 0

Query: 3   ITSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCV 62
           + S   YDRVL YFDEDGDGKISPSELRNRLGLIGGEL  AEAE AVESLDSDGDGLLCV
Sbjct: 4   VKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLDSDGDGLLCV 63

Query: 63  GDIXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRR 122
           GDI      XXXXX       F LYD+EGCGFITPK+LK+ML+KLGE KST ECK+MIRR
Sbjct: 64  GDIERLLEVXXXXXLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRR 123

Query: 123 FDLNGDGLISFEEFQIMMA 142
           FDLNGDGLISFEEFQIMMA
Sbjct: 124 FDLNGDGLISFEEFQIMMA 142

BLAST of Carg20175 vs. TrEMBL
Match: tr|M5WYD1|M5WYD1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019709mg PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.9e-40
Identity = 91/139 (65.47%), Postives = 102/139 (73.38%), Query Frame = 0

Query: 3   ITSARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCV 62
           +TS R +DRVL YFDEDGDGKISPSELR  LG +GGELL  EAEVAVE LDSDGDGLL +
Sbjct: 1   MTSNRGFDRVLSYFDEDGDGKISPSELRRGLGFMGGELLQKEAEVAVELLDSDGDGLLGL 60

Query: 63  GD-IXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIR 122
            D +                  F +YD EGCGFITPKSLKRML  LGE++S  ECKVMI 
Sbjct: 61  EDLVCLMEGGEEEEKVKGLRDAFQVYDVEGCGFITPKSLKRMLSSLGESRSIDECKVMIN 120

Query: 123 RFDLNGDGLISFEEFQIMM 141
           +FDLNGDG+ISFEEF+IMM
Sbjct: 121 QFDLNGDGVISFEEFRIMM 139

BLAST of Carg20175 vs. TrEMBL
Match: tr|A0A2P6P4Z4|A0A2P6P4Z4_ROSCH (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7g0190421 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.0e-40
Identity = 90/137 (65.69%), Postives = 101/137 (73.72%), Query Frame = 0

Query: 5   SARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGD 64
           S R ++RV  YFDEDGDGKISPSELRNRLGL+GGE+L  EAEVAV  LDSDGDGLL + D
Sbjct: 3   SNRGFERVFSYFDEDGDGKISPSELRNRLGLMGGEMLQNEAEVAVGFLDSDGDGLLGLDD 62

Query: 65  -IXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRF 124
            +                  F +YD EGCGFITPKSLKRML +LGE+KS  ECKVMI +F
Sbjct: 63  LVGLMEGGEEEEKMKGLREAFEVYDEEGCGFITPKSLKRMLNRLGESKSIEECKVMINQF 122

Query: 125 DLNGDGLISFEEFQIMM 141
           DLNGDGLISFEEF +MM
Sbjct: 123 DLNGDGLISFEEFTVMM 139

BLAST of Carg20175 vs. TrEMBL
Match: tr|A0A251P8V2|A0A251P8V2_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G152700 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.9e-39
Identity = 90/137 (65.69%), Postives = 100/137 (72.99%), Query Frame = 0

Query: 5   SARHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGD 64
           S R +DRVL YFDEDGDGKISPSELR  LG +GGELL  EAEVAVE LDSDGDGLL + D
Sbjct: 3   SNRGFDRVLSYFDEDGDGKISPSELRRGLGFMGGELLQKEAEVAVELLDSDGDGLLGLED 62

Query: 65  -IXXXXXXXXXXXXXXXXXXFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRF 124
            +                  F +YD EGCGFITPKSLKRML  LGE++S  ECKVMI +F
Sbjct: 63  LVCLMEGGEEEEKVKGLRDAFQVYDVEGCGFITPKSLKRMLSSLGESRSIDECKVMINQF 122

Query: 125 DLNGDGLISFEEFQIMM 141
           DLNGDG+ISFEEF+IMM
Sbjct: 123 DLNGDGVISFEEFRIMM 139

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022925940.11.1e-5998.58putative calcium-binding protein CML19 [Cucurbita moschata][more]
XP_022966390.12.5e-5997.87probable calcium-binding protein CML31 [Cucurbita maxima][more]
XP_023529455.14.2e-5997.87probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo][more]
XP_008464450.11.6e-5075.35PREDICTED: putative calcium-binding protein CML19 [Cucumis melo][more]
XP_004138054.12.8e-5078.42PREDICTED: putative calcium-binding protein CML19 [Cucumis sativus] >KGN63491.1 ... [more]
Match NameE-valueIdentityDescription
AT1G76650.12.7e-2343.70calmodulin-like 38[more]
AT1G76640.11.7e-2241.30Calcium-binding EF-hand family protein[more]
AT5G42380.16.2e-2040.91calmodulin like 37[more]
AT3G50770.18.9e-1936.50calmodulin-like 41[more]
AT1G24620.18.3e-1735.97EF hand calcium-binding protein family[more]
Match NameE-valueIdentityDescription
sp|Q9SRE6|CML38_ARATH4.8e-2243.70Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1[more]
sp|Q8RYJ8|CML19_ORYSJ1.4e-2143.36Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9SRE7|CML39_ARATH3.1e-2141.30Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1[more]
sp|Q8RYJ9|CML23_ORYSJ2.2e-1948.32Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9FIH9|CML37_ARATH1.1e-1840.91Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CN12|A0A1S3CN12_CUCME1.1e-5075.35putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 P... [more]
tr|A0A0A0LU40|A0A0A0LU40_CUCSA1.8e-5078.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1[more]
tr|M5WYD1|M5WYD1_PRUPE2.9e-4065.47Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019709mg PE=4 SV=1[more]
tr|A0A2P6P4Z4|A0A2P6P4Z4_ROSCH5.0e-4065.69Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7... [more]
tr|A0A251P8V2|A0A251P8V2_PRUPE1.9e-3965.69Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G152700 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20175-RACarg20175-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 79..107
e-value: 0.1
score: 21.7
coord: 8..36
e-value: 0.1
score: 21.7
coord: 115..141
e-value: 0.078
score: 22.1
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 12..31
e-value: 8.8E-6
score: 24.9
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 78..140
e-value: 1.2E-12
score: 48.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 4..39
score: 12.087
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 40..74
score: 5.698
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 75..110
score: 11.473
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 111..141
score: 14.262
IPR002048EF-hand domainCDDcd00051EFhcoord: 79..141
e-value: 4.69217E-15
score: 64.8765
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 2..69
e-value: 1.1E-9
score: 40.4
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 70..141
e-value: 3.2E-22
score: 80.6
NoneNo IPR availablePANTHERPTHR43996:SF8SUBFAMILY NOT NAMEDcoord: 7..140
NoneNo IPR availablePANTHERPTHR43996FAMILY NOT NAMEDcoord: 7..140
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 17..29
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 124..136
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 11..140

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg20175Carg10289Silver-seed gourdcarcarB396
Carg20175Carg15572Silver-seed gourdcarcarB399
The following block(s) are covering this gene:
GeneOrganismBlock
Carg20175Silver-seed gourdcarcarB446
Carg20175Silver-seed gourdcarcarB484