Carg10289 (gene) Silver-seed gourd

NameCarg10289
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCalcium-binding protein
LocationCucurbita_argyrosperma_scaffold_047 : 253572 .. 254060 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGAATTGACCAAACATCCAAACTCGAGAAAATCCGAAATTTTCCAATCTCGAATCGAAATGGAAATCAACAGTTCGGCACAGTACGGTCGTGTGTTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGGTCGGATTGATGGGCGGAGAGCTGCTGCTGGGGGAAGCGGAGATGGCGGTGGAGTCGCTCGACTCGGACGGCGACGGGTTGCTGTGCGCGGGCGATTTCGAACGGCTTCTGGACGTGGGGGAGGAGGAGAAATTGAAGGATTTGAAAGAGGCGTTTGGTCTGTACGATTGCGAAGGTTGCGGGTTCATAACTCCGAAGAGCTTGAAGAGAATGCTGAGAAAATTGGGGGAATCGAAGTCCACAGCAGAGTGCAAGTTGATGATCGGTCAGTTTGATTTGAACGGGGATGGTTTGATTAGCTTTGAGGAGTTTCAGATAATGATGGCGTAA

mRNA sequence

ATGCCTGAATTGACCAAACATCCAAACTCGAGAAAATCCGAAATTTTCCAATCTCGAATCGAAATGGAAATCAACAGTTCGGCACAGTACGGTCGTGTGTTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGGTCGGATTGATGGGCGGAGAGCTGCTGCTGGGGGAAGCGGAGATGGCGGTGGAGTCGCTCGACTCGGACGGCGACGGGTTGCTGTGCGCGGGCGATTTCGAACGGCTTCTGGACGTGGGGGAGGAGGAGAAATTGAAGGATTTGAAAGAGGCGTTTGGTCTGTACGATTGCGAAGGTTGCGGGTTCATAACTCCGAAGAGCTTGAAGAGAATGCTGAGAAAATTGGGGGAATCGAAGTCCACAGCAGAGTGCAAGTTGATGATCGGTCAGTTTGATTTGAACGGGGATGGTTTGATTAGCTTTGAGGAGTTTCAGATAATGATGGCGTAA

Coding sequence (CDS)

ATGCCTGAATTGACCAAACATCCAAACTCGAGAAAATCCGAAATTTTCCAATCTCGAATCGAAATGGAAATCAACAGTTCGGCACAGTACGGTCGTGTGTTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGGTCGGATTGATGGGCGGAGAGCTGCTGCTGGGGGAAGCGGAGATGGCGGTGGAGTCGCTCGACTCGGACGGCGACGGGTTGCTGTGCGCGGGCGATTTCGAACGGCTTCTGGACGTGGGGGAGGAGGAGAAATTGAAGGATTTGAAAGAGGCGTTTGGTCTGTACGATTGCGAAGGTTGCGGGTTCATAACTCCGAAGAGCTTGAAGAGAATGCTGAGAAAATTGGGGGAATCGAAGTCCACAGCAGAGTGCAAGTTGATGATCGGTCAGTTTGATTTGAACGGGGATGGTTTGATTAGCTTTGAGGAGTTTCAGATAATGATGGCGTAA

Protein sequence

MPELTKHPNSRKSEIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA
BLAST of Carg10289 vs. NCBI nr
Match: XP_022921811.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 295.8 bits (756), Expect = 8.8e-77
Identity = 160/162 (98.77%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE 60
           MPELTKHPNSRKSE+FQSRIEMEIN+SAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE
Sbjct: 4   MPELTKHPNSRKSEVFQSRIEMEINNSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE 63

Query: 61  LLLGEAEMAVESLDSDGDGLLCAGDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKS 120
           LLLGEAEMAVESLDSDGDGLLCAGDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKS
Sbjct: 64  LLLGEAEMAVESLDSDGDGLLCAGDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKS 123

Query: 121 LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 163
           LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA
Sbjct: 124 LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 165

BLAST of Carg10289 vs. NCBI nr
Match: XP_023516478.1 (probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 254.2 bits (648), Expect = 2.9e-64
Identity = 129/141 (91.49%), Postives = 131/141 (92.91%), Query Frame = 0

Query: 22  MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 81
           MEIN+SAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAV+SLDSDGDGLL
Sbjct: 1   MEINNSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVQSLDSDGDGLL 60

Query: 82  CAGDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 141
           CAGDFERL      XX    KEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI
Sbjct: 61  CAGDFERLLDVGEEXXLNDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 120

Query: 142 GQFDLNGDGLISFEEFQIMMA 163
           GQFDLNGDGLISFEEFQIMMA
Sbjct: 121 GQFDLNGDGLISFEEFQIMMA 141

BLAST of Carg10289 vs. NCBI nr
Match: XP_022987451.1 (putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 243.8 bits (621), Expect = 4.0e-61
Identity = 123/141 (87.23%), Postives = 124/141 (87.94%), Query Frame = 0

Query: 22  MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 81
           MEINSSA YGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL
Sbjct: 1   MEINSSAHYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 60

Query: 82  CAGDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 141
           CAGDFERL            KEAF LYDCEGCGFITPKSLK+MLRKLGE KSTAECKLMI
Sbjct: 61  CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKSLKKMLRKLGERKSTAECKLMI 120

Query: 142 GQFDLNGDGLISFEEFQIMMA 163
           G FDLNGDGLISFEEFQ MMA
Sbjct: 121 GVFDLNGDGLISFEEFQKMMA 141

BLAST of Carg10289 vs. NCBI nr
Match: XP_008464450.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo])

HSP 1 Score: 218.0 bits (554), Expect = 2.3e-53
Identity = 112/142 (78.87%), Postives = 118/142 (83.10%), Query Frame = 0

Query: 22  MEIN-SSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGL 81
           MEIN S AQY RVLSYFDEDGDGKISPSELRNR+GL+GGELL  EAE AVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 82  LCAGDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLM 141
           LCAGD ERL            KEAF LYD EGCGFITPK+LK+MLRKLGE KST ECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 142 IGQFDLNGDGLISFEEFQIMMA 163
           I +FDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

BLAST of Carg10289 vs. NCBI nr
Match: XP_004138054.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis sativus] >KGN63491.1 hypothetical protein Csa_1G002110 [Cucumis sativus])

HSP 1 Score: 212.2 bits (539), Expect = 1.3e-51
Identity = 111/139 (79.86%), Postives = 118/139 (84.89%), Query Frame = 0

Query: 24  INSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCA 83
           + S AQY RVLSYFDEDGDGKISPSELRNR+GL+GGEL   EAE AVESLDSDGDGLLC 
Sbjct: 4   VKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLDSDGDGLLCV 63

Query: 84  GDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQ 143
           GD ERL   XXXXX    KEAF LYD EGCGFITPK+LK+MLRKLGE KST ECK+MI +
Sbjct: 64  GDIERLLEVXXXXXLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRR 123

Query: 144 FDLNGDGLISFEEFQIMMA 163
           FDLNGDGLISFEEFQIMMA
Sbjct: 124 FDLNGDGLISFEEFQIMMA 142

BLAST of Carg10289 vs. TAIR10
Match: AT1G76650.1 (calmodulin-like 38)

HSP 1 Score: 110.2 bits (274), Expect = 1.2e-24
Identity = 61/130 (46.92%), Postives = 81/130 (62.31%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERL-XX 92
           V SY D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   +F +L   
Sbjct: 47  VFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLIKV 106

Query: 93  XXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGL 152
                     K AF LY  EG   ITP+SLK ML+KLGES++T +C++MI  FDLN DG+
Sbjct: 107 DDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNADGV 166

Query: 153 ISFEEFQIMM 162
           +SF+EF +MM
Sbjct: 167 LSFDEFALMM 176

BLAST of Carg10289 vs. TAIR10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 104.8 bits (260), Expect = 5.2e-23
Identity = 59/133 (44.36%), Postives = 79/133 (59.40%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLXXX 92
           V +Y D + DG+IS  EL+     +G ++   EAE AV+  D DGDG+L   +F  L   
Sbjct: 26  VFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLIKG 85

Query: 93  XXXXXXXXXK----EAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNG 152
                    K    EAF +Y  +G   ITP SLK ML KLGES++T +CK+MI  FDLN 
Sbjct: 86  NDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDLNA 145

Query: 153 DGLISFEEFQIMM 162
           DG++SF+EF +MM
Sbjct: 146 DGVLSFDEFALMM 158

BLAST of Carg10289 vs. TAIR10
Match: AT5G42380.1 (calmodulin like 37)

HSP 1 Score: 102.1 bits (253), Expect = 3.4e-22
Identity = 67/174 (38.51%), Postives = 92/174 (52.87%), Query Frame = 0

Query: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYG----------RVLSYFDEDGDGKISPSEL 60
           +  L K  +S++SE  ++  +    SS   G           V  Y D + DGKIS  EL
Sbjct: 11  LSRLYKKVSSKRSESSRNLEDESRTSSNSSGSSSLNVNELRTVFDYMDANSDGKISGEEL 70

Query: 61  RNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERL---XXXXXXXXXXXXKEAFGL 120
           ++ V L+GG L   E E  V++ D DGDG +   +F +L               KEAFG+
Sbjct: 71  QSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEGEDGSDEERRKELKEAFGM 130

Query: 121 YDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMM 162
           Y  EG  FIT  SL+R L +LGES +   CK+MI  FD N DG++SF+EF +MM
Sbjct: 131 YVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDDGVLSFDEFVLMM 184

BLAST of Carg10289 vs. TAIR10
Match: AT3G50770.1 (calmodulin-like 41)

HSP 1 Score: 97.4 bits (241), Expect = 8.4e-21
Identity = 57/143 (39.86%), Postives = 82/143 (57.34%), Query Frame = 0

Query: 26  SSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGD 85
           S  +  +V S+FD DGDGKIS  ELR+  G +G  +    A+ A+  +D+D DG L   D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 86  F------ERLXXXXXXXXXXXXKEAFGLYDCE-GCGFITPKSLKRMLRKLGESKSTAECK 145
           F        L            K AF +++ E G G ITPK L++ML KLGES++  EC+
Sbjct: 121 FVGLMTRRDLYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECE 180

Query: 146 LMIGQFDLNGDGLISFEEFQIMM 162
            MI  +D++G+G++ F EF+ MM
Sbjct: 181 AMIKFYDIDGNGILDFHEFRQMM 203

BLAST of Carg10289 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 92.0 bits (227), Expect = 3.5e-19
Identity = 54/131 (41.22%), Postives = 72/131 (54.96%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERL--X 92
           V   FD +GDGKIS  EL   +  +G E+   E E A+  +D  GDG +   +F  L   
Sbjct: 41  VFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTK 100

Query: 93  XXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDG 152
                      K+AF +YD +G G I+ + L  +LR LG+  S AEC+ MIG  D +GDG
Sbjct: 101 GMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDG 160

Query: 153 LISFEEFQIMM 162
            I FEEF+IMM
Sbjct: 161 TIDFEEFKIMM 171

BLAST of Carg10289 vs. Swiss-Prot
Match: sp|Q8RYJ8|CML19_ORYSJ (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 9.1e-25
Identity = 67/143 (46.85%), Postives = 88/143 (61.54%), Query Frame = 0

Query: 24  INSSAQYGRVLSYFDEDGDGKISPSELRNRV-GLMGGELLLGEAEMAVESLDSDGDGLLC 83
           + ++A++ RV S FD D DGKIS +ELR  +   +G ++   EAE  V S D+D DGLL 
Sbjct: 2   VAATAEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLD 61

Query: 84  AGDFER----LXXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECK 143
             +F +    L             EAF +Y+ EG G ITP SLKRML KLG  +   EC+
Sbjct: 62  EEEFTKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQ 121

Query: 144 LMIGQFDLNGDGLISFEEFQIMM 162
            MI +FDL+GDG+ISFEEF+IMM
Sbjct: 122 TMICRFDLDGDGVISFEEFKIMM 144

BLAST of Carg10289 vs. Swiss-Prot
Match: sp|Q9SRE6|CML38_ARATH (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.2e-23
Identity = 61/130 (46.92%), Postives = 81/130 (62.31%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERL-XX 92
           V SY D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   +F +L   
Sbjct: 47  VFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLIKV 106

Query: 93  XXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGL 152
                     K AF LY  EG   ITP+SLK ML+KLGES++T +C++MI  FDLN DG+
Sbjct: 107 DDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNADGV 166

Query: 153 ISFEEFQIMM 162
           +SF+EF +MM
Sbjct: 167 LSFDEFALMM 176

BLAST of Carg10289 vs. Swiss-Prot
Match: sp|Q8RYJ9|CML23_ORYSJ (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-22
Identity = 74/147 (50.34%), Postives = 95/147 (64.63%), Query Frame = 0

Query: 26  SSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESL-DSDGDGLLCAG 85
           +S ++ RV   FD+DGDGKIS +ELR  V    GE +  E   A+ +L D+DGDGLL   
Sbjct: 3   ASDEFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEE 62

Query: 86  DFERL----------XXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKST 145
           +F RL          XXXXXXXXX   +EAF +Y+ EG G ITP SLK ML KLG     
Sbjct: 63  EFVRLVTEMXXXXXXXXXXXXXXXRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDV 122

Query: 146 AECKLMIGQFDLNGDGLISFEEFQIMM 162
           AEC+ MI +FD+NGDG+++F+EF+ MM
Sbjct: 123 AECQAMICRFDMNGDGVLTFDEFKTMM 149

BLAST of Carg10289 vs. Swiss-Prot
Match: sp|Q9SRE7|CML39_ARATH (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 9.4e-22
Identity = 59/133 (44.36%), Postives = 79/133 (59.40%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLXXX 92
           V +Y D + DG+IS  EL+     +G ++   EAE AV+  D DGDG+L   +F  L   
Sbjct: 26  VFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLIKG 85

Query: 93  XXXXXXXXXK----EAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNG 152
                    K    EAF +Y  +G   ITP SLK ML KLGES++T +CK+MI  FDLN 
Sbjct: 86  NDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDLNA 145

Query: 153 DGLISFEEFQIMM 162
           DG++SF+EF +MM
Sbjct: 146 DGVLSFDEFALMM 158

BLAST of Carg10289 vs. Swiss-Prot
Match: sp|Q9FIH9|CML37_ARATH (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 6.1e-21
Identity = 67/174 (38.51%), Postives = 92/174 (52.87%), Query Frame = 0

Query: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYG----------RVLSYFDEDGDGKISPSEL 60
           +  L K  +S++SE  ++  +    SS   G           V  Y D + DGKIS  EL
Sbjct: 11  LSRLYKKVSSKRSESSRNLEDESRTSSNSSGSSSLNVNELRTVFDYMDANSDGKISGEEL 70

Query: 61  RNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERL---XXXXXXXXXXXXKEAFGL 120
           ++ V L+GG L   E E  V++ D DGDG +   +F +L               KEAFG+
Sbjct: 71  QSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEGEDGSDEERRKELKEAFGM 130

Query: 121 YDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMM 162
           Y  EG  FIT  SL+R L +LGES +   CK+MI  FD N DG++SF+EF +MM
Sbjct: 131 YVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDDGVLSFDEFVLMM 184

BLAST of Carg10289 vs. TrEMBL
Match: tr|A0A1S3CN12|A0A1S3CN12_CUCME (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.5e-53
Identity = 112/142 (78.87%), Postives = 118/142 (83.10%), Query Frame = 0

Query: 22  MEIN-SSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGL 81
           MEIN S AQY RVLSYFDEDGDGKISPSELRNR+GL+GGELL  EAE AVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 82  LCAGDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLM 141
           LCAGD ERL            KEAF LYD EGCGFITPK+LK+MLRKLGE KST ECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 142 IGQFDLNGDGLISFEEFQIMMA 163
           I +FDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

BLAST of Carg10289 vs. TrEMBL
Match: tr|A0A0A0LU40|A0A0A0LU40_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.5e-52
Identity = 111/139 (79.86%), Postives = 118/139 (84.89%), Query Frame = 0

Query: 24  INSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCA 83
           + S AQY RVLSYFDEDGDGKISPSELRNR+GL+GGEL   EAE AVESLDSDGDGLLC 
Sbjct: 4   VKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLDSDGDGLLCV 63

Query: 84  GDFERLXXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQ 143
           GD ERL   XXXXX    KEAF LYD EGCGFITPK+LK+MLRKLGE KST ECK+MI +
Sbjct: 64  GDIERLLEVXXXXXLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRR 123

Query: 144 FDLNGDGLISFEEFQIMMA 163
           FDLNGDGLISFEEFQIMMA
Sbjct: 124 FDLNGDGLISFEEFQIMMA 142

BLAST of Carg10289 vs. TrEMBL
Match: tr|A0A2P6P4Z4|A0A2P6P4Z4_ROSCH (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7g0190421 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-41
Identity = 93/139 (66.91%), Postives = 104/139 (74.82%), Query Frame = 0

Query: 24  INSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCA 83
           + S+  + RV SYFDEDGDGKISPSELRNR+GLMGGE+L  EAE+AV  LDSDGDGLL  
Sbjct: 1   MQSNRGFERVFSYFDEDGDGKISPSELRNRLGLMGGEMLQNEAEVAVGFLDSDGDGLLGL 60

Query: 84  GDFERL-XXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIG 143
            D   L             +EAF +YD EGCGFITPKSLKRML +LGESKS  ECK+MI 
Sbjct: 61  DDLVGLMEGGEEEEKMKGLREAFEVYDEEGCGFITPKSLKRMLNRLGESKSIEECKVMIN 120

Query: 144 QFDLNGDGLISFEEFQIMM 162
           QFDLNGDGLISFEEF +MM
Sbjct: 121 QFDLNGDGLISFEEFTVMM 139

BLAST of Carg10289 vs. TrEMBL
Match: tr|M5WYD1|M5WYD1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019709mg PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-39
Identity = 91/139 (65.47%), Postives = 103/139 (74.10%), Query Frame = 0

Query: 24  INSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCA 83
           + S+  + RVLSYFDEDGDGKISPSELR  +G MGGELL  EAE+AVE LDSDGDGLL  
Sbjct: 1   MTSNRGFDRVLSYFDEDGDGKISPSELRRGLGFMGGELLQKEAEVAVELLDSDGDGLLGL 60

Query: 84  GDFERL-XXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIG 143
            D   L             ++AF +YD EGCGFITPKSLKRML  LGES+S  ECK+MI 
Sbjct: 61  EDLVCLMEGGEEEEKVKGLRDAFQVYDVEGCGFITPKSLKRMLSSLGESRSIDECKVMIN 120

Query: 144 QFDLNGDGLISFEEFQIMM 162
           QFDLNGDG+ISFEEF+IMM
Sbjct: 121 QFDLNGDGVISFEEFRIMM 139

BLAST of Carg10289 vs. TrEMBL
Match: tr|A0A251P8V2|A0A251P8V2_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G152700 PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-39
Identity = 91/139 (65.47%), Postives = 103/139 (74.10%), Query Frame = 0

Query: 24  INSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCA 83
           + S+  + RVLSYFDEDGDGKISPSELR  +G MGGELL  EAE+AVE LDSDGDGLL  
Sbjct: 1   MKSNRGFDRVLSYFDEDGDGKISPSELRRGLGFMGGELLQKEAEVAVELLDSDGDGLLGL 60

Query: 84  GDFERL-XXXXXXXXXXXXKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIG 143
            D   L             ++AF +YD EGCGFITPKSLKRML  LGES+S  ECK+MI 
Sbjct: 61  EDLVCLMEGGEEEEKVKGLRDAFQVYDVEGCGFITPKSLKRMLSSLGESRSIDECKVMIN 120

Query: 144 QFDLNGDGLISFEEFQIMM 162
           QFDLNGDG+ISFEEF+IMM
Sbjct: 121 QFDLNGDGVISFEEFRIMM 139

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022921811.18.8e-7798.77putative calcium-binding protein CML19 [Cucurbita moschata][more]
XP_023516478.12.9e-6491.49probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo][more]
XP_022987451.14.0e-6187.23putative calcium-binding protein CML19 [Cucurbita maxima][more]
XP_008464450.12.3e-5378.87PREDICTED: putative calcium-binding protein CML19 [Cucumis melo][more]
XP_004138054.11.3e-5179.86PREDICTED: putative calcium-binding protein CML19 [Cucumis sativus] >KGN63491.1 ... [more]
Match NameE-valueIdentityDescription
AT1G76650.11.2e-2446.92calmodulin-like 38[more]
AT1G76640.15.2e-2344.36Calcium-binding EF-hand family protein[more]
AT5G42380.13.4e-2238.51calmodulin like 37[more]
AT3G50770.18.4e-2139.86calmodulin-like 41[more]
AT1G24620.13.5e-1941.22EF hand calcium-binding protein family[more]
Match NameE-valueIdentityDescription
sp|Q8RYJ8|CML19_ORYSJ9.1e-2546.85Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9SRE6|CML38_ARATH2.2e-2346.92Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1[more]
sp|Q8RYJ9|CML23_ORYSJ1.9e-2250.34Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9SRE7|CML39_ARATH9.4e-2244.36Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1[more]
sp|Q9FIH9|CML37_ARATH6.1e-2138.51Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CN12|A0A1S3CN12_CUCME1.5e-5378.87putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 P... [more]
tr|A0A0A0LU40|A0A0A0LU40_CUCSA8.5e-5279.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1[more]
tr|A0A2P6P4Z4|A0A2P6P4Z4_ROSCH3.0e-4166.91Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7... [more]
tr|M5WYD1|M5WYD1_PRUPE1.3e-3965.47Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019709mg PE=4 SV=1[more]
tr|A0A251P8V2|A0A251P8V2_PRUPE1.3e-3965.47Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G152700 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10289-RACarg10289-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 65..93
e-value: 59.0
score: 3.5
coord: 100..128
e-value: 0.27
score: 20.3
coord: 136..162
e-value: 1.1
score: 17.3
coord: 29..57
e-value: 1.2
score: 17.2
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 35..52
e-value: 4.2E-5
score: 22.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 99..161
e-value: 7.4E-13
score: 48.7
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 132..162
score: 12.672
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 61..95
score: 5.894
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 25..60
score: 11.25
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 96..131
score: 10.775
IPR002048EF-hand domainCDDcd00051EFhcoord: 100..162
e-value: 1.72223E-13
score: 61.0245
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 92..162
e-value: 1.0E-20
score: 75.7
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..91
e-value: 1.5E-9
score: 40.0
NoneNo IPR availablePANTHERPTHR43996FAMILY NOT NAMEDcoord: 27..161
NoneNo IPR availablePANTHERPTHR43996:SF8SUBFAMILY NOT NAMEDcoord: 27..161
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 145..157
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 38..50
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 25..161

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg10289Carg15572Silver-seed gourdcarcarB387
Carg10289Carg20175Silver-seed gourdcarcarB396