Bhi02G000399 (gene) Wax gourd

Overview
NameBhi02G000399
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCalcium-binding protein
Locationchr2 : 10125430 .. 10126907 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATTTTCATTTTTGACCCAATTAAACACGTTCTTCTTTTTTAATCCCTTTTTTCTTTTCTCTTTTGAATTTTATACCATTCCGAGGCTTTCCTTCGCCGCAGTTTCCAAAAATATCCCGAAGCCTATAAATGGAAACCCCAAACCCTGAATTGACCAAAAATCCAAATTCAGAGAAAAATCCACAAAACTCCTTTCTAAAAATTCTCCAATCCCGAATTGAAATGGAGATCAACTGTGCGGCACAGTACGATCGCGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATTTCGCCGTCGGAGCTGAGGAATCGGCTGGGATTAATGGGCGGAGAGCTGCTGCAGGCGGAGGCTGAGGCGGCGGTGGAATCGCTGGACTCGGACGGCGATGGATTGCTGTGCGCCGGCGATTTCGAACGGCTACTGGAGGTTGGGGAGGAAGAGAAATTGAAGGATTTGAAAGAGGCGTTTGCTCTGTACGATTGTGAAGGTTGCGGGTTCATTACTCCGAAAAATTTGAAGAAAATGCTAAGAAAATTGGGGGAAAGAAAATCCACCGAGGAGTGTAAATTGATGATTCGTCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCTTAACTCTGTACATATCTTCATTTCTTTTTCTTTCTTACATCGACATATTCTTTTTAATTTCATTTTTTTGGATATCTGATTATTCAATTCATTTTAGTTGTTGAATTTGGGGCATTTCCCATTTGTATAAATACACAAACACATTTCTATCTGTAGACTTTTGAGATTATATGAATAAATAATGACGTGAGTTTAGCTGGGTTGAATATTCGATCTCATTTATATGGATAAATATATATGTATTTGGGTTATGAATTAAGGATGAATTGTTTATAGTCTCATTCCATGTGTTTTGAGTCTTCCAAAGAAACTATTTATGTTTATGGTTACACGTGATTAGATATTAAAATTTTTAGCATGAAAGAATTGACTCTTCTAAATGAAGAAAAAAGGGTATTTCCTTAATCCTTCCCAAGAAAGATTTTTGTTTCTTCCTATGAACTATGAAGGAGGTCTTGTCACTGTCAAATGGAAGCAGAGTTGATTAGTAATGATGATATTACGAAATAATCAACTCTCTTTGTCTCTGTCTCTTTCTTTCCTGTTTAAGAAGAAGATAGAAAAATTTTAAACATGTACTCTGTCTCTGTCTCTCTCTAGAAATAGGTCATTTCCACTTTAGTTACGTGCAAATTTAGAATTTCGATTTATGTAGGTACTTCCTTTTGAATGACATCTGTTCTCCTTTTAGTTTTTTTTGTATGTATGCATCAAGGAGTTCTCTAATCTCTAGGTGTATATGAGTTGTACTGGTTCGTGTGGATGGATTCTTTGTCGAACAGAAGTGATCACGTATGTCAATAGGGATATTTAAAAAACCTTTATCGAGGG

mRNA sequence

CCCATTTTCATTTTTGACCCAATTAAACACGTTCTTCTTTTTTAATCCCTTTTTTCTTTTCTCTTTTGAATTTTATACCATTCCGAGGCTTTCCTTCGCCGCAGTTTCCAAAAATATCCCGAAGCCTATAAATGGAAACCCCAAACCCTGAATTGACCAAAAATCCAAATTCAGAGAAAAATCCACAAAACTCCTTTCTAAAAATTCTCCAATCCCGAATTGAAATGGAGATCAACTGTGCGGCACAGTACGATCGCGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATTTCGCCGTCGGAGCTGAGGAATCGGCTGGGATTAATGGGCGGAGAGCTGCTGCAGGCGGAGGCTGAGGCGGCGGTGGAATCGCTGGACTCGGACGGCGATGGATTGCTGTGCGCCGGCGATTTCGAACGGCTACTGGAGGTTGGGGAGGAAGAGAAATTGAAGGATTTGAAAGAGGCGTTTGCTCTGTACGATTGTGAAGGTTGCGGGTTCATTACTCCGAAAAATTTGAAGAAAATGCTAAGAAAATTGGGGGAAAGAAAATCCACCGAGGAGTGTAAATTGATGATTCGTCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCTTAACTCTGTACATATCTTCATTTCTTTTTCTTTCTTACATCGACATATTCTTTTTAATTTCATTTTTTTGGATATCTGATTATTCAATTCATTTTAGTTGTTGAATTTGGGGCATTTCCCATTTGTATAAATACACAAACACATTTCTATCTGTAGACTTTTGAGATTATATGAATAAATAATGACGTGAGTTTAGCTGGGTTGAATATTCGATCTCATTTATATGGATAAATATATATGTATTTGGGTTATGAATTAAGGATGAATTGTTTATAGTCTCATTCCATGTGTTTTGAGTCTTCCAAAGAAACTATTTATGTTTATGGTTACACGTGATTAGATATTAAAATTTTTAGCATGAAAGAATTGACTCTTCTAAATGAAGAAAAAAGGGTATTTCCTTAATCCTTCCCAAGAAAGATTTTTGTTTCTTCCTATGAACTATGAAGGAGGTCTTGTCACTGTCAAATGGAAGCAGAGTTGATTAGTAATGATGATATTACGAAATAATCAACTCTCTTTGTCTCTGTCTCTTTCTTTCCTGTTTAAGAAGAAGATAGAAAAATTTTAAACATGTACTCTGTCTCTGTCTCTCTCTAGAAATAGGTCATTTCCACTTTAGTTACGTGCAAATTTAGAATTTCGATTTATGTAGGTACTTCCTTTTGAATGACATCTGTTCTCCTTTTAGTTTTTTTTGTATGTATGCATCAAGGAGTTCTCTAATCTCTAGGTGTATATGAGTTGTACTGGTTCGTGTGGATGGATTCTTTGTCGAACAGAAGTGATCACGTATGTCAATAGGGATATTTAAAAAACCTTTATCGAGGG

Coding sequence (CDS)

ATGGAAACCCCAAACCCTGAATTGACCAAAAATCCAAATTCAGAGAAAAATCCACAAAACTCCTTTCTAAAAATTCTCCAATCCCGAATTGAAATGGAGATCAACTGTGCGGCACAGTACGATCGCGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATTTCGCCGTCGGAGCTGAGGAATCGGCTGGGATTAATGGGCGGAGAGCTGCTGCAGGCGGAGGCTGAGGCGGCGGTGGAATCGCTGGACTCGGACGGCGATGGATTGCTGTGCGCCGGCGATTTCGAACGGCTACTGGAGGTTGGGGAGGAAGAGAAATTGAAGGATTTGAAAGAGGCGTTTGCTCTGTACGATTGTGAAGGTTGCGGGTTCATTACTCCGAAAAATTTGAAGAAAATGCTAAGAAAATTGGGGGAAAGAAAATCCACCGAGGAGTGTAAATTGATGATTCGTCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCTTAA

Protein sequence

METPNPELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA
Homology
[Close]
BLAST of Bhi02G000399 vs. Swiss-Prot
Match: sp|Q8RYJ8|CML19_ORYSJ (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.4e-30
Identity = 75/143 (52.45%), Postives = 98/143 (68.53%), Query Frame = 0 

Query: 34  INCAAQYDRVLSYFDEDGDGKISPSELRNRL-GLMGGELLQAEAEAAVESLDSDGDGLLC 93
           +   A++ RV S FD D DGKIS +ELR  +   +G ++   EAEA V S D+D DGLL 
Sbjct: 2   VAATAEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLD 61

Query: 94  AGDFERL---LEVG-EEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECK 153
             +F +L   LE+G EEE+ + L EAF +Y+ EG G ITP +LK+ML KLG  +  EEC+
Sbjct: 62  EEEFTKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQ 121

Query: 154 LMIRRFDLNGDGLISFEEFQIMM 172
            MI RFDL+GDG+ISFEEF+IMM
Sbjct: 122 TMICRFDLDGDGVISFEEFKIMM 144

[Close]
BLAST of Bhi02G000399 vs. Swiss-Prot
Match: sp|Q9SRE6|CML38_ARATH (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 6.5e-29
Identity = 66/134 (49.25%), Postives = 93/134 (69.40%), Query Frame = 0 

Query: 39  QYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFER 98
           + + V SY D + DG+ISP EL+     +G +L   EA AAV   D+DGDG+L   +F +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 99  LLEV-GEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLN 158
           L++V  EEEK  +LK AF LY  EG   ITP++LK ML+KLGE ++T++C++MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 159 GDGLISFEEFQIMM 172
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 176

[Close]
BLAST of Bhi02G000399 vs. Swiss-Prot
Match: sp|Q9SRE7|CML39_ARATH (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.6e-27
Identity = 67/163 (41.10%), Postives = 101/163 (61.96%), Query Frame = 0 

Query: 13  NSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELL 72
           N+++   +SF+K L+ +           + V +Y D + DG+IS  EL+     +G ++ 
Sbjct: 3   NTQRQLSSSFMKFLEEK-------NRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMS 62

Query: 73  QAEAEAAVESLDSDGDGLLCAGDFERLL----EVGEEEKLKDLKEAFALYDCEGCGFITP 132
             EAEAAV+  D DGDG+L   +F  L+    E  EEEK + + EAF +Y  +G   ITP
Sbjct: 63  DEEAEAAVKLSDIDGDGMLDINEFALLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITP 122

Query: 133 KNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMM 172
            +LK ML KLGE ++T++CK+MI+ FDLN DG++SF+EF +MM
Sbjct: 123 GSLKMMLMKLGESRTTDDCKVMIQAFDLNADGVLSFDEFALMM 158

[Close]
BLAST of Bhi02G000399 vs. Swiss-Prot
Match: sp|Q9FIH9|CML37_ARATH (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 9.7e-25
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 0 

Query: 43  VLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLE- 102
           V  Y D + DGKIS  EL++ + L+GG L   E E  V++ D DGDG +   +F +L+E 
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 103 --VGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGD 162
               +EE+ K+LKEAF +Y  EG  FIT  +L++ L +LGE  + + CK+MIR FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 163 GLISFEEFQIMM 172
           G++SF+EF +MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]
BLAST of Bhi02G000399 vs. Swiss-Prot
Match: sp|Q8RYJ9|CML23_ORYSJ (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.1e-23
Identity = 62/144 (43.06%), Postives = 87/144 (60.42%), Query Frame = 0 

Query: 39  QYDRVLSYFDEDGDGKISPSELRNRL-GLMGGELLQAEAEAAVESLDSDGDGLLCAGDFE 98
           ++ RV   FD+DGDGKIS +ELR  +   +G ++   E +A +   D+DGDGLL   +F 
Sbjct: 6   EFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFV 65

Query: 99  RLL----------EVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEEC 158
           RL+                  + L+EAFA+Y+ EG G ITP +LK ML KLG      EC
Sbjct: 66  RLVTEMXXXXXXXXXXXXXXXRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAEC 125

Query: 159 KLMIRRFDLNGDGLISFEEFQIMM 172
           + MI RFD+NGDG+++F+EF+ MM
Sbjct: 126 QAMICRFDMNGDGVLTFDEFKTMM 149

[Close]
BLAST of Bhi02G000399 vs. TAIR10
Match: AT1G76650.1 (calmodulin-like 38)

HSP 1 Score: 128.6 bits (322), Expect = 3.6e-30
Identity = 66/134 (49.25%), Postives = 93/134 (69.40%), Query Frame = 0 

Query: 39  QYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFER 98
           + + V SY D + DG+ISP EL+     +G +L   EA AAV   D+DGDG+L   +F +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 99  LLEV-GEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLN 158
           L++V  EEEK  +LK AF LY  EG   ITP++LK ML+KLGE ++T++C++MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 159 GDGLISFEEFQIMM 172
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 176

[Close]
BLAST of Bhi02G000399 vs. TAIR10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 124.0 bits (310), Expect = 8.9e-29
Identity = 67/163 (41.10%), Postives = 101/163 (61.96%), Query Frame = 0 

Query: 13  NSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELL 72
           N+++   +SF+K L+ +           + V +Y D + DG+IS  EL+     +G ++ 
Sbjct: 3   NTQRQLSSSFMKFLEEK-------NRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMS 62

Query: 73  QAEAEAAVESLDSDGDGLLCAGDFERLL----EVGEEEKLKDLKEAFALYDCEGCGFITP 132
             EAEAAV+  D DGDG+L   +F  L+    E  EEEK + + EAF +Y  +G   ITP
Sbjct: 63  DEEAEAAVKLSDIDGDGMLDINEFALLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITP 122

Query: 133 KNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMM 172
            +LK ML KLGE ++T++CK+MI+ FDLN DG++SF+EF +MM
Sbjct: 123 GSLKMMLMKLGESRTTDDCKVMIQAFDLNADGVLSFDEFALMM 158

[Close]
BLAST of Bhi02G000399 vs. TAIR10
Match: AT5G42380.1 (calmodulin like 37)

HSP 1 Score: 114.8 bits (286), Expect = 5.4e-26
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 0 

Query: 43  VLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLE- 102
           V  Y D + DGKIS  EL++ + L+GG L   E E  V++ D DGDG +   +F +L+E 
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 103 --VGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGD 162
               +EE+ K+LKEAF +Y  EG  FIT  +L++ L +LGE  + + CK+MIR FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 163 GLISFEEFQIMM 172
           G++SF+EF +MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]
BLAST of Bhi02G000399 vs. TAIR10
Match: AT3G50770.1 (calmodulin-like 41)

HSP 1 Score: 105.5 bits (262), Expect = 3.3e-23
Identity = 59/137 (43.07%), Postives = 86/137 (62.77%), Query Frame = 0 

Query: 42  RVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLE 101
           +V S+FD DGDGKIS  ELR+  G +G  +    A+ A+  +D+D DG L   DF  L+ 
Sbjct: 67  QVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMT 126

Query: 102 ------VGEEEKLKDLKEAFALYDCE-GCGFITPKNLKKMLRKLGERKSTEECKLMIRRF 161
                  GE +   +LK AF +++ E G G ITPK L+KML KLGE ++  EC+ MI+ +
Sbjct: 127 RRDLYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFY 186

Query: 162 DLNGDGLISFEEFQIMM 172
           D++G+G++ F EF+ MM
Sbjct: 187 DIDGNGILDFHEFRQMM 203

[Close]
BLAST of Bhi02G000399 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 99.8 bits (247), Expect = 1.8e-21
Identity = 55/135 (40.74%), Postives = 82/135 (60.74%), Query Frame = 0 

Query: 39  QYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFER 98
           + + V   FD +GDGKIS  EL   +  +G E+ + E E A+  +D  GDG +   +F  
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 99  LLEVG--EEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDL 158
           L   G  + + L++LK+AF++YD +G G I+ + L ++LR LG+  S  EC+ MI   D 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 159 NGDGLISFEEFQIMM 172
           +GDG I FEEF+IMM
Sbjct: 157 DGDGTIDFEEFKIMM 171

[Close]
BLAST of Bhi02G000399 vs. TrEMBL
Match: tr|A0A1S3CN12|A0A1S3CN12_CUCME (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 5.3e-68
Identity = 135/142 (95.07%), Postives = 137/142 (96.48%), Query Frame = 0 

Query: 32  MEIN-CAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGL 91
           MEIN   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGELLQAEAEAAVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 92  LCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLM 151
           LCAGD ERLLEVGEEEKLKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKSTEECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 152 IRRFDLNGDGLISFEEFQIMMA 173
           IRRFDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

[Close]
BLAST of Bhi02G000399 vs. TrEMBL
Match: tr|A0A0A0LU40|A0A0A0LU40_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-62
Identity = 124/139 (89.21%), Postives = 127/139 (91.37%), Query Frame = 0 

Query: 34  INCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCA 93
           +   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGEL QAEAEAAVESLDSDGDGLLC 
Sbjct: 4   VKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLDSDGDGLLCV 63

Query: 94  GDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRR 153
           GD ERLLEV     LKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKSTEECK+MIRR
Sbjct: 64  GDIERLLEVXXXXXLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRR 123

Query: 154 FDLNGDGLISFEEFQIMMA 173
           FDLNGDGLISFEEFQIMMA
Sbjct: 124 FDLNGDGLISFEEFQIMMA 142

[Close]
BLAST of Bhi02G000399 vs. TrEMBL
Match: tr|A0A2P6P4Z4|A0A2P6P4Z4_ROSCH (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7g0190421 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 5.1e-47
Identity = 99/133 (74.44%), Postives = 112/133 (84.21%), Query Frame = 0 

Query: 40  YDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERL 99
           ++RV SYFDEDGDGKISPSELRNRLGLMGGE+LQ EAE AV  LDSDGDGLL   D   L
Sbjct: 7   FERVFSYFDEDGDGKISPSELRNRLGLMGGEMLQNEAEVAVGFLDSDGDGLLGLDDLVGL 66

Query: 100 LEVG-EEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNG 159
           +E G EEEK+K L+EAF +YD EGCGFITPK+LK+ML +LGE KS EECK+MI +FDLNG
Sbjct: 67  MEGGEEEEKMKGLREAFEVYDEEGCGFITPKSLKRMLNRLGESKSIEECKVMINQFDLNG 126

Query: 160 DGLISFEEFQIMM 172
           DGLISFEEF +MM
Sbjct: 127 DGLISFEEFTVMM 139

[Close]
BLAST of Bhi02G000399 vs. TrEMBL
Match: tr|M5WYD1|M5WYD1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019709mg PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 9.7e-46
Identity = 97/133 (72.93%), Postives = 111/133 (83.46%), Query Frame = 0 

Query: 40  YDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERL 99
           +DRVLSYFDEDGDGKISPSELR  LG MGGELLQ EAE AVE LDSDGDGLL   D   L
Sbjct: 7   FDRVLSYFDEDGDGKISPSELRRGLGFMGGELLQKEAEVAVELLDSDGDGLLGLEDLVCL 66

Query: 100 LEVG-EEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNG 159
           +E G EEEK+K L++AF +YD EGCGFITPK+LK+ML  LGE +S +ECK+MI +FDLNG
Sbjct: 67  MEGGEEEEKVKGLRDAFQVYDVEGCGFITPKSLKRMLSSLGESRSIDECKVMINQFDLNG 126

Query: 160 DGLISFEEFQIMM 172
           DG+ISFEEF+IMM
Sbjct: 127 DGVISFEEFRIMM 139

[Close]
BLAST of Bhi02G000399 vs. TrEMBL
Match: tr|A0A251P8V2|A0A251P8V2_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G152700 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 9.7e-46
Identity = 97/133 (72.93%), Postives = 111/133 (83.46%), Query Frame = 0 

Query: 40  YDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERL 99
           +DRVLSYFDEDGDGKISPSELR  LG MGGELLQ EAE AVE LDSDGDGLL   D   L
Sbjct: 7   FDRVLSYFDEDGDGKISPSELRRGLGFMGGELLQKEAEVAVELLDSDGDGLLGLEDLVCL 66

Query: 100 LEVG-EEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNG 159
           +E G EEEK+K L++AF +YD EGCGFITPK+LK+ML  LGE +S +ECK+MI +FDLNG
Sbjct: 67  MEGGEEEEKVKGLRDAFQVYDVEGCGFITPKSLKRMLSSLGESRSIDECKVMINQFDLNG 126

Query: 160 DGLISFEEFQIMM 172
           DG+ISFEEF+IMM
Sbjct: 127 DGVISFEEFRIMM 139

[Close]
BLAST of Bhi02G000399 vs. NCBI nr
Match: XP_008464450.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo])

HSP 1 Score: 266.2 bits (679), Expect = 8.0e-68
Identity = 135/142 (95.07%), Postives = 137/142 (96.48%), Query Frame = 0 

Query: 32  MEIN-CAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGL 91
           MEIN   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGELLQAEAEAAVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 92  LCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLM 151
           LCAGD ERLLEVGEEEKLKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKSTEECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 152 IRRFDLNGDGLISFEEFQIMMA 173
           IRRFDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

[Close]
BLAST of Bhi02G000399 vs. NCBI nr
Match: XP_022987451.1 (putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 255.0 bits (650), Expect = 1.8e-64
Identity = 128/141 (90.78%), Postives = 131/141 (92.91%), Query Frame = 0 

Query: 32  MEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLL 91
           MEIN +A Y RVLSYFDEDGDGKISPSELRNR+GLMGGELL  EAE AVESLDSDGDGLL
Sbjct: 1   MEINSSAHYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 60

Query: 92  CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMI 151
           CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPK+LKKMLRKLGERKST ECKLMI
Sbjct: 61  CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKSLKKMLRKLGERKSTAECKLMI 120

Query: 152 RRFDLNGDGLISFEEFQIMMA 173
             FDLNGDGLISFEEFQ MMA
Sbjct: 121 GVFDLNGDGLISFEEFQKMMA 141

[Close]
BLAST of Bhi02G000399 vs. NCBI nr
Match: XP_022921811.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 249.6 bits (636), Expect = 7.7e-63
Identity = 129/167 (77.25%), Postives = 138/167 (82.63%), Query Frame = 0 

Query: 6   PELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLG 65
           PELTK+PNS K+      ++ QSRIEMEIN +AQY RVLSYFDEDGDGKISPSELRNR+G
Sbjct: 5   PELTKHPNSRKS------EVFQSRIEMEINNSAQYGRVLSYFDEDGDGKISPSELRNRVG 64

Query: 66  LMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGF 125
           LMGGELL  EAE AVESLDSDGDGLLCAGDFERL            KEAF LYDCEGCGF
Sbjct: 65  LMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLXXXXXXXXXXXXKEAFGLYDCEGCGF 124

Query: 126 ITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA 173
           ITPK+LK+MLRKLGE KST ECKLMI +FDLNGDGLISFEEFQIMMA
Sbjct: 125 ITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 165

[Close]
BLAST of Bhi02G000399 vs. NCBI nr
Match: XP_004138054.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis sativus] >KGN63491.1 hypothetical protein Csa_1G002110 [Cucumis sativus])

HSP 1 Score: 248.4 bits (633), Expect = 1.7e-62
Identity = 124/139 (89.21%), Postives = 127/139 (91.37%), Query Frame = 0 

Query: 34  INCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCA 93
           +   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGEL QAEAEAAVESLDSDGDGLLC 
Sbjct: 4   VKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLDSDGDGLLCV 63

Query: 94  GDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRR 153
           GD ERLLEV     LKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKSTEECK+MIRR
Sbjct: 64  GDIERLLEVXXXXXLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRR 123

Query: 154 FDLNGDGLISFEEFQIMMA 173
           FDLNGDGLISFEEFQIMMA
Sbjct: 124 FDLNGDGLISFEEFQIMMA 142

[Close]
BLAST of Bhi02G000399 vs. NCBI nr
Match: XP_023516478.1 (probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 246.5 bits (628), Expect = 6.5e-62
Identity = 122/141 (86.52%), Postives = 129/141 (91.49%), Query Frame = 0 

Query: 32  MEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLL 91
           MEIN +AQY RVLSYFDEDGDGKISPSELRNR+GLMGGELL  EAE AV+SLDSDGDGLL
Sbjct: 1   MEINNSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVQSLDSDGDGLL 60

Query: 92  CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMI 151
           CAGDFERLL+VGEE  L DLKEAF LYDCEGCGFITPK+LK+MLRKLGE KST ECKLMI
Sbjct: 61  CAGDFERLLDVGEEXXLNDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 120

Query: 152 RRFDLNGDGLISFEEFQIMMA 173
            +FDLNGDGLISFEEFQIMMA
Sbjct: 121 GQFDLNGDGLISFEEFQIMMA 141

The following BLAST results are available for this feature:
BLAST of Bhi02G000399 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8RYJ8|CML19_ORYSJ3.4e-3052.45Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9SRE6|CML38_ARATH6.5e-2949.25Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1[more]
sp|Q9SRE7|CML39_ARATH1.6e-2741.10Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1[more]
sp|Q9FIH9|CML37_ARATH9.7e-2545.45Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1[more]
sp|Q8RYJ9|CML23_ORYSJ4.1e-2343.06Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
BLAST of Bhi02G000399 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G76650.13.6e-3049.25calmodulin-like 38[more]
AT1G76640.18.9e-2941.10Calcium-binding EF-hand family protein[more]
AT5G42380.15.4e-2645.45calmodulin like 37[more]
AT3G50770.13.3e-2343.07calmodulin-like 41[more]
AT1G24620.11.8e-2140.74EF hand calcium-binding protein family[more]
BLAST of Bhi02G000399 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3CN12|A0A1S3CN12_CUCME5.3e-6895.07putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 P... [more]
tr|A0A0A0LU40|A0A0A0LU40_CUCSA1.1e-6289.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1[more]
tr|A0A2P6P4Z4|A0A2P6P4Z4_ROSCH5.1e-4774.44Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7... [more]
tr|M5WYD1|M5WYD1_PRUPE9.7e-4672.93Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019709mg PE=4 SV=1[more]
tr|A0A251P8V2|A0A251P8V2_PRUPE9.7e-4672.93Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G152700 PE=4 SV=1[more]
BLAST of Bhi02G000399 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008464450.18.0e-6895.07PREDICTED: putative calcium-binding protein CML19 [Cucumis melo][more]
XP_022987451.11.8e-6490.78putative calcium-binding protein CML19 [Cucurbita maxima][more]
XP_022921811.17.7e-6377.25putative calcium-binding protein CML19 [Cucurbita moschata][more]
XP_004138054.11.7e-6289.21PREDICTED: putative calcium-binding protein CML19 [Cucumis sativus] >KGN63491.1 ... [more]
XP_023516478.16.5e-6286.52probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000399Bhi02M000399mRNA


InterPro
Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 75..103
e-value: 45.0
score: 4.4
coord: 39..67
e-value: 0.13
score: 21.4
coord: 146..172
e-value: 0.2
score: 20.8
coord: 110..138
e-value: 0.047
score: 22.8
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 43..62
e-value: 2.8E-5
score: 23.3
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 109..171
e-value: 1.1E-14
score: 54.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 106..141
score: 12.087
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 35..70
score: 11.501
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 142..172
score: 14.318
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 71..105
score: 7.735
IPR002048EF-hand domainCDDcd00051EFhcoord: 110..172
e-value: 2.23539E-15
score: 66.4173
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 100..172
e-value: 3.8E-22
score: 80.3
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 2..99
e-value: 7.4E-12
score: 47.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR43996FAMILY NOT NAMEDcoord: 37..171
NoneNo IPR availablePANTHERPTHR43996:SF8SUBFAMILY NOT NAMEDcoord: 37..171
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 48..60
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 155..167
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 35..171

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Bhi02G000399Csa1G002110Cucumber (Chinese Long) v2cuwgoB078
Bhi02G000399MELO3C026214Melon (DHL92) v3.5.1mewgoB311
Bhi02G000399ClCG06G017800Watermelon (Charleston Gray)wcgwgoB549
Bhi02G000399CSPI01G00620Wild cucumber (PI 183967)cpiwgoB080
Bhi02G000399Cucsa.157410Cucumber (Gy14) v1cgywgoB305
Bhi02G000399CmaCh11G000410Cucurbita maxima (Rimu)cmawgoB0153
Bhi02G000399CmaCh10G000700Cucurbita maxima (Rimu)cmawgoB0081
Bhi02G000399CmoCh11G000420Cucurbita moschata (Rifu)cmowgoB0148
Bhi02G000399CmoCh10G000650Cucurbita moschata (Rifu)cmowgoB0074
Bhi02G000399Lsi06G016340Bottle gourd (USVL1VR-Ls)lsiwgoB526
Bhi02G000399Cp4.1LG18g08810Cucurbita pepo (Zucchini)cpewgoB0469
Bhi02G000399Cp4.1LG04g16040Cucurbita pepo (Zucchini)cpewgoB0898
Bhi02G000399CsGy1G000630Cucumber (Gy14) v2cgybwgoB076
Bhi02G000399MELO3C026214.2Melon (DHL92) v3.6.1medwgoB309
Bhi02G000399Carg20175Silver-seed gourdcarwgoB1226
Bhi02G000399Carg10289Silver-seed gourdcarwgoB0447
Bhi02G000399CsaV3_1G000650Cucumber (Chinese Long) v3cucwgoB086
Bhi02G000399Cla019269Watermelon (97103) v1wgowmB686
Bhi02G000399Cla97C06G127530Watermelon (97103) v2wgowmbB657
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi02G000399Cucurbita pepo (Zucchini)cpewgoB0459
Bhi02G000399Cucurbita pepo (Zucchini)cpewgoB0883
Bhi02G000399Bottle gourd (USVL1VR-Ls)lsiwgoB501
Bhi02G000399Melon (DHL92) v3.6.1medwgoB291
Bhi02G000399Melon (DHL92) v3.5.1mewgoB291
Bhi02G000399Silver-seed gourdcarwgoB1227