Carg15572 (gene) Silver-seed gourd

NameCarg15572
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCalcium-binding protein
LocationCucurbita_argyrosperma_scaffold_059 : 289851 .. 290276 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAAGTTATCGTGTTACAAGAGAGTGTTCGATCATTTTGACATGGATGGAGATGGGAAGATTTCTCCGGTCGAGGTACGGGAGTGTATAGCAGCGATATCAGGGGCGGAGGTGTCGATGGTGGAGGCAGAGGAGGTGGTGGCCGCATTCGACGTGGATGGAGACGGTGGACTTGACGTGGAGGAGTTTGAGAGGTTCGTCGACGACGGAGAGGAGGAGGAGAAGGTGAAAGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGGTCGGGATTGATTACGGCGGTGAGCTTGAGGAGGACGTTGAAGAAATTAGGAGAGTTCGTAAGTTTGGAGGATTGTATGGCTATGATTGCCAAGTTTGACCTTGACGGTGACGGTGTTCTTAGTTTTGATGAATTTAAGGTTATGATGGGATGA

mRNA sequence

ATGGATAAGTTATCGTGTTACAAGAGAGTGTTCGATCATTTTGACATGGATGGAGATGGGAAGATTTCTCCGGTCGAGGTACGGGAGTGTATAGCAGCGATATCAGGGGCGGAGGTGTCGATGGTGGAGGCAGAGGAGGTGGTGGCCGCATTCGACGTGGATGGAGACGGTGGACTTGACGTGGAGGAGTTTGAGAGGTTCGTCGACGACGGAGAGGAGGAGGAGAAGGTGAAAGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGGTCGGGATTGATTACGGCGGTGAGCTTGAGGAGGACGTTGAAGAAATTAGGAGAGTTCGTAAGTTTGGAGGATTGTATGGCTATGATTGCCAAGTTTGACCTTGACGGTGACGGTGTTCTTAGTTTTGATGAATTTAAGGTTATGATGGGATGA

Coding sequence (CDS)

ATGGATAAGTTATCGTGTTACAAGAGAGTGTTCGATCATTTTGACATGGATGGAGATGGGAAGATTTCTCCGGTCGAGGTACGGGAGTGTATAGCAGCGATATCAGGGGCGGAGGTGTCGATGGTGGAGGCAGAGGAGGTGGTGGCCGCATTCGACGTGGATGGAGACGGTGGACTTGACGTGGAGGAGTTTGAGAGGTTCGTCGACGACGGAGAGGAGGAGGAGAAGGTGAAAGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGGTCGGGATTGATTACGGCGGTGAGCTTGAGGAGGACGTTGAAGAAATTAGGAGAGTTCGTAAGTTTGGAGGATTGTATGGCTATGATTGCCAAGTTTGACCTTGACGGTGACGGTGTTCTTAGTTTTGATGAATTTAAGGTTATGATGGGATGA

Protein sequence

MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDLDGDGVLSFDEFKVMMG
BLAST of Carg15572 vs. NCBI nr
Match: XP_022928328.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 265.8 bits (678), Expect = 8.5e-68
Identity = 137/141 (97.16%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKLSCYKRVFDHFD DGDGKISP EVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD
Sbjct: 1   MDKLSCYKRVFDHFDTDGDGKISPFEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           VEEFERFVDDGEEEEKVKELREAFKMYEM+GSGLITAVSLRRTLKKLGEFVSLEDCMAMI
Sbjct: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMDGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMMG 142
           AKFDLD DGVLSFDEFKVMMG
Sbjct: 121 AKFDLDSDGVLSFDEFKVMMG 141

BLAST of Carg15572 vs. NCBI nr
Match: KYP32675.1 (Putative calcium-binding protein CML19 [Cajanus cajan])

HSP 1 Score: 172.9 bits (437), Expect = 7.5e-40
Identity = 89/140 (63.57%), Postives = 112/140 (80.00%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDK+S Y RVF+HFD +GDGKISP E+R+C+ A+ G E+S+ EAE VV   D D DG + 
Sbjct: 1   MDKMSQYVRVFNHFDENGDGKISPSELRQCVEAV-GGELSLEEAEAVVELLDSDKDGLVG 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           +++F RF ++GEEEEKVK+LREAFKMYEMEG G IT  SL+R L +LGE  S+++C  MI
Sbjct: 61  LDDFVRFAEEGEEEEKVKDLREAFKMYEMEGCGCITPKSLKRMLGRLGECKSIDECKVMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           A+FDLDGDGVLSFDEF+VMM
Sbjct: 121 ARFDLDGDGVLSFDEFRVMM 139

BLAST of Carg15572 vs. NCBI nr
Match: XP_020208193.1 (putative calcium-binding protein CML19 [Cajanus cajan])

HSP 1 Score: 172.9 bits (437), Expect = 7.5e-40
Identity = 89/140 (63.57%), Postives = 112/140 (80.00%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDK+S Y RVF+HFD +GDGKISP E+R+C+ A+ G E+S+ EAE VV   D D DG + 
Sbjct: 2   MDKMSQYVRVFNHFDENGDGKISPSELRQCVEAV-GGELSLEEAEAVVELLDSDKDGLVG 61

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           +++F RF ++GEEEEKVK+LREAFKMYEMEG G IT  SL+R L +LGE  S+++C  MI
Sbjct: 62  LDDFVRFAEEGEEEEKVKDLREAFKMYEMEGCGCITPKSLKRMLGRLGECKSIDECKVMI 121

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           A+FDLDGDGVLSFDEF+VMM
Sbjct: 122 ARFDLDGDGVLSFDEFRVMM 140

BLAST of Carg15572 vs. NCBI nr
Match: RDY13437.1 (putative calcium-binding protein CML19, partial [Mucuna pruriens])

HSP 1 Score: 166.8 bits (421), Expect = 5.4e-38
Identity = 86/138 (62.32%), Postives = 110/138 (79.71%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKL+ Y  VF+HFD +GDGKISP E+R+C+ AI G E+S+ EAE V    D D DG + 
Sbjct: 1   MDKLTQYGLVFNHFDENGDGKISPSELRQCVEAI-GGELSLEEAEAVAKLLDSDRDGLVG 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           +++F RFV++GEEEEKVK+LREAFKMYEMEG G IT  SL+R L +LGE  ++++C AMI
Sbjct: 61  LDDFVRFVEEGEEEEKVKDLREAFKMYEMEGCGCITPKSLKRMLGRLGECKNIDECQAMI 120

Query: 121 AKFDLDGDGVLSFDEFKV 139
           A+FDLDGDGVLSFDEF++
Sbjct: 121 ARFDLDGDGVLSFDEFRI 137

BLAST of Carg15572 vs. NCBI nr
Match: XP_014508533.1 (putative calcium-binding protein CML19 [Vigna radiata var. radiata])

HSP 1 Score: 164.5 bits (415), Expect = 2.7e-37
Identity = 85/140 (60.71%), Postives = 111/140 (79.29%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           M+K+S Y RVF+HFD +GDG+IS  E+R+C+ AI G +VS  EAE +V   D D DG + 
Sbjct: 2   MEKVSQYVRVFNHFDENGDGRISASELRQCVEAI-GGKVSWAEAEALVEVLDSDKDGLVG 61

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           +++F RFV++GEEEEKVK+L+EAFKMYEMEG G IT  SL+R L +LGE  S+++C  MI
Sbjct: 62  LDDFVRFVEEGEEEEKVKDLKEAFKMYEMEGCGCITPKSLKRMLGRLGECKSIDECQTMI 121

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           A+FDL+GDGVLSFDEF+VMM
Sbjct: 122 ARFDLNGDGVLSFDEFRVMM 140

BLAST of Carg15572 vs. TAIR10
Match: AT5G42380.1 (calmodulin like 37)

HSP 1 Score: 127.9 bits (320), Expect = 5.0e-30
Identity = 72/135 (53.33%), Postives = 94/135 (69.63%), Query Frame = 0

Query: 8   KRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERF 67
           + VFD+ D + DGKIS  E++ C++ + GA +S  E EEVV   DVDGDG +D EEF + 
Sbjct: 51  RTVFDYMDANSDGKISGEELQSCVSLLGGA-LSSREVEEVVKTSDVDGDGFIDFEEFLKL 110

Query: 68  V--DDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDL 127
           +  +DG +EE+ KEL+EAF MY MEG   ITA SLRRTL +LGE  +++ C  MI  FD 
Sbjct: 111 MEGEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQ 170

Query: 128 DGDGVLSFDEFKVMM 141
           + DGVLSFDEF +MM
Sbjct: 171 NDDGVLSFDEFVLMM 184

BLAST of Carg15572 vs. TAIR10
Match: AT1G76650.1 (calmodulin-like 38)

HSP 1 Score: 110.5 bits (275), Expect = 8.3e-25
Identity = 65/139 (46.76%), Postives = 87/139 (62.59%), Query Frame = 0

Query: 2   DKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDV 61
           DK    + VF + D + DG+ISP E+++    + G ++S  EA   V   D DGDG LD 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDF 98

Query: 62  EEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIA 121
           EEF + +   +EEEK  EL+ AF++Y  EG   IT  SL+  LKKLGE  + +DC  MI+
Sbjct: 99  EEFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMIS 158

Query: 122 KFDLDGDGVLSFDEFKVMM 141
            FDL+ DGVLSFDEF +MM
Sbjct: 159 AFDLNADGVLSFDEFALMM 176

BLAST of Carg15572 vs. TAIR10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 102.4 bits (254), Expect = 2.3e-22
Identity = 61/134 (45.52%), Postives = 84/134 (62.69%), Query Frame = 0

Query: 10  VFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERFV- 69
           VF + D + DG+IS  E+++    + G ++S  EAE  V   D+DGDG LD+ EF   + 
Sbjct: 26  VFAYMDANRDGRISAEELKKSFKTL-GEQMSDEEAEAAVKLSDIDGDGMLDINEFALLIK 85

Query: 70  --DDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDLD 129
             D+  EEEK +++ EAF+MY  +G   IT  SL+  L KLGE  + +DC  MI  FDL+
Sbjct: 86  GNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDLN 145

Query: 130 GDGVLSFDEFKVMM 141
            DGVLSFDEF +MM
Sbjct: 146 ADGVLSFDEFALMM 158

BLAST of Carg15572 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 99.8 bits (247), Expect = 1.5e-21
Identity = 57/135 (42.22%), Postives = 89/135 (65.93%), Query Frame = 0

Query: 9   RVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEF-ERF 68
           RVF  FD +GDGKI+  E+++   ++ G  V   E  E++A  DV+GDG +D++EF   +
Sbjct: 8   RVFQMFDKNGDGKIAKNELKDFFKSV-GIMVPENEINEMIAKMDVNGDGAMDIDEFGSLY 67

Query: 69  VDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLG--EFVSLEDCMAMIAKFDL 128
            +  EE+E+ +++REAF++++  G G IT   LR  L  +G  +  +LEDC  MI+K D+
Sbjct: 68  QEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDV 127

Query: 129 DGDGVLSFDEFKVMM 141
           DGDG+++F EFK MM
Sbjct: 128 DGDGMVNFKEFKQMM 141

BLAST of Carg15572 vs. TAIR10
Match: AT3G50770.1 (calmodulin-like 41)

HSP 1 Score: 93.6 bits (231), Expect = 1.1e-19
Identity = 60/140 (42.86%), Postives = 83/140 (59.29%), Query Frame = 0

Query: 8   KRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEF--- 67
           ++VF HFD DGDGKIS  E+R    ++ G  +S   A+E +   D D DG L  E+F   
Sbjct: 66  RQVFSHFDSDGDGKISAFELRHYFGSV-GEYISHEAAQEAINEVDTDADGSLGFEDFVGL 125

Query: 68  ---ERFVDDGEEEEKVKELREAFKMYEME-GSGLITAVSLRRTLKKLGEFVSLEDCMAMI 127
                   DGE +    EL+ AF+M+E+E GSG IT   L++ L KLGE  +  +C AMI
Sbjct: 126 MTRRDLYGDGEVDGD-GELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMI 185

Query: 128 AKFDLDGDGVLSFDEFKVMM 141
             +D+DG+G+L F EF+ MM
Sbjct: 186 KFYDIDGNGILDFHEFRQMM 203

BLAST of Carg15572 vs. Swiss-Prot
Match: sp|Q8RYJ8|CML19_ORYSJ (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.1e-32
Identity = 73/137 (53.28%), Postives = 96/137 (70.07%), Query Frame = 0

Query: 7   YKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFER 66
           ++RVF  FD D DGKIS  E+R C+ A  G ++S  EAE +V++ D D DG LD EEF +
Sbjct: 8   FRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEFTK 67

Query: 67  F---VDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKF 126
               ++ G+EEE+ + L EAF+MYEMEG G IT  SL+R L KLG    +E+C  MI +F
Sbjct: 68  LAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMICRF 127

Query: 127 DLDGDGVLSFDEFKVMM 141
           DLDGDGV+SF+EFK+MM
Sbjct: 128 DLDGDGVISFEEFKIMM 144

BLAST of Carg15572 vs. Swiss-Prot
Match: sp|Q9FIH9|CML37_ARATH (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 9.1e-29
Identity = 72/135 (53.33%), Postives = 94/135 (69.63%), Query Frame = 0

Query: 8   KRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERF 67
           + VFD+ D + DGKIS  E++ C++ + GA +S  E EEVV   DVDGDG +D EEF + 
Sbjct: 51  RTVFDYMDANSDGKISGEELQSCVSLLGGA-LSSREVEEVVKTSDVDGDGFIDFEEFLKL 110

Query: 68  V--DDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDL 127
           +  +DG +EE+ KEL+EAF MY MEG   ITA SLRRTL +LGE  +++ C  MI  FD 
Sbjct: 111 MEGEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQ 170

Query: 128 DGDGVLSFDEFKVMM 141
           + DGVLSFDEF +MM
Sbjct: 171 NDDGVLSFDEFVLMM 184

BLAST of Carg15572 vs. Swiss-Prot
Match: sp|Q8RYJ9|CML23_ORYSJ (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 6.5e-27
Identity = 69/143 (48.25%), Postives = 89/143 (62.24%), Query Frame = 0

Query: 7   YKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFER 66
           ++RVF  FD DGDGKIS  E+R C+ A  G ++   E + ++A  D DGDG LD EEF R
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  FVDD---------GEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCM 126
            V +                + LREAF MYEMEG G IT +SL+  L KLG  + + +C 
Sbjct: 67  LVTEMXXXXXXXXXXXXXXXRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 AMIAKFDLDGDGVLSFDEFKVMM 141
           AMI +FD++GDGVL+FDEFK MM
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMM 149

BLAST of Carg15572 vs. Swiss-Prot
Match: sp|Q9SRE6|CML38_ARATH (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-23
Identity = 65/139 (46.76%), Postives = 87/139 (62.59%), Query Frame = 0

Query: 2   DKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDV 61
           DK    + VF + D + DG+ISP E+++    + G ++S  EA   V   D DGDG LD 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDF 98

Query: 62  EEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIA 121
           EEF + +   +EEEK  EL+ AF++Y  EG   IT  SL+  LKKLGE  + +DC  MI+
Sbjct: 99  EEFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMIS 158

Query: 122 KFDLDGDGVLSFDEFKVMM 141
            FDL+ DGVLSFDEF +MM
Sbjct: 159 AFDLNADGVLSFDEFALMM 176

BLAST of Carg15572 vs. Swiss-Prot
Match: sp|Q9SRE7|CML39_ARATH (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.1e-21
Identity = 61/134 (45.52%), Postives = 84/134 (62.69%), Query Frame = 0

Query: 10  VFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERFV- 69
           VF + D + DG+IS  E+++    + G ++S  EAE  V   D+DGDG LD+ EF   + 
Sbjct: 26  VFAYMDANRDGRISAEELKKSFKTL-GEQMSDEEAEAAVKLSDIDGDGMLDINEFALLIK 85

Query: 70  --DDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDLD 129
             D+  EEEK +++ EAF+MY  +G   IT  SL+  L KLGE  + +DC  MI  FDL+
Sbjct: 86  GNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDLN 145

Query: 130 GDGVLSFDEFKVMM 141
            DGVLSFDEF +MM
Sbjct: 146 ADGVLSFDEFALMM 158

BLAST of Carg15572 vs. TrEMBL
Match: tr|A0A151QQW8|A0A151QQW8_CAJCA (Putative calcium-binding protein CML19 OS=Cajanus cajan OX=3821 GN=KK1_046572 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.0e-40
Identity = 89/140 (63.57%), Postives = 112/140 (80.00%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDK+S Y RVF+HFD +GDGKISP E+R+C+ A+ G E+S+ EAE VV   D D DG + 
Sbjct: 1   MDKMSQYVRVFNHFDENGDGKISPSELRQCVEAV-GGELSLEEAEAVVELLDSDKDGLVG 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           +++F RF ++GEEEEKVK+LREAFKMYEMEG G IT  SL+R L +LGE  S+++C  MI
Sbjct: 61  LDDFVRFAEEGEEEEKVKDLREAFKMYEMEGCGCITPKSLKRMLGRLGECKSIDECKVMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           A+FDLDGDGVLSFDEF+VMM
Sbjct: 121 ARFDLDGDGVLSFDEFRVMM 139

BLAST of Carg15572 vs. TrEMBL
Match: tr|A0A1S3UR21|A0A1S3UR21_VIGRR (putative calcium-binding protein CML19 OS=Vigna radiata var. radiata OX=3916 GN=LOC106768082 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.8e-37
Identity = 85/140 (60.71%), Postives = 111/140 (79.29%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           M+K+S Y RVF+HFD +GDG+IS  E+R+C+ AI G +VS  EAE +V   D D DG + 
Sbjct: 2   MEKVSQYVRVFNHFDENGDGRISASELRQCVEAI-GGKVSWAEAEALVEVLDSDKDGLVG 61

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           +++F RFV++GEEEEKVK+L+EAFKMYEMEG G IT  SL+R L +LGE  S+++C  MI
Sbjct: 62  LDDFVRFVEEGEEEEKVKDLKEAFKMYEMEGCGCITPKSLKRMLGRLGECKSIDECQTMI 121

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           A+FDL+GDGVLSFDEF+VMM
Sbjct: 122 ARFDLNGDGVLSFDEFRVMM 140

BLAST of Carg15572 vs. TrEMBL
Match: tr|A0A2C9UHG0|A0A2C9UHG0_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_15G159100 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.8e-37
Identity = 87/134 (64.93%), Postives = 105/134 (78.36%), Query Frame = 0

Query: 7   YKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFER 66
           Y+RVF HFD +GDGKISP E++ C+ A+ G E+S+ EAE  V   D DGDG L  EEF R
Sbjct: 10  YERVFSHFDENGDGKISPWELQRCVGAV-GGELSLAEAEAAVEYSDSDGDGLLCFEEFVR 69

Query: 67  FVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDLD 126
           FVD GEEEEKVK+L+EAFKMYEME SG IT  SL+R L++LG+  S+E C  MIA+FDL+
Sbjct: 70  FVDSGEEEEKVKDLKEAFKMYEMEESGCITPKSLKRMLRRLGQATSIEACKIMIAQFDLN 129

Query: 127 GDGVLSFDEFKVMM 141
           GDGVL+FDEFKVMM
Sbjct: 130 GDGVLNFDEFKVMM 142

BLAST of Carg15572 vs. TrEMBL
Match: tr|W9SWS7|W9SWS7_9ROSA (Calcium-binding protein CML38 OS=Morus notabilis OX=981085 GN=L484_017621 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.9e-37
Identity = 84/140 (60.00%), Postives = 108/140 (77.14%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKL+ Y+RVF+HFD DGDGKISP+E++ C+ +I G E+S  EAE  +   D DGD  L 
Sbjct: 1   MDKLTQYERVFNHFDADGDGKISPLELQTCVGSI-GGELSEAEAEAAMDYLDSDGDRLLG 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           +++F +F++ G EEEKVK+L+EAFKMYEMEG G IT  SL+R L +LGE  S++DC  MI
Sbjct: 61  LDDFVKFLEGGREEEKVKDLKEAFKMYEMEGCGCITPKSLKRMLSRLGESKSIDDCKVMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           A+FDL GDGVL+FDEF VMM
Sbjct: 121 ARFDLKGDGVLNFDEFMVMM 139

BLAST of Carg15572 vs. TrEMBL
Match: tr|A0A2I4ERQ5|A0A2I4ERQ5_9ROSI (putative calcium-binding protein CML19 OS=Juglans regia OX=51240 GN=LOC108992079 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-36
Identity = 82/140 (58.57%), Postives = 109/140 (77.86%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDK + Y+RVF++FD +GDGKISP E+++C+ AI   E+S+ EAE VV   D DGD  + 
Sbjct: 1   MDKHTQYERVFNYFDDNGDGKISPSELQQCVEAIGTGELSLEEAEAVVELLDSDGDSMVG 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           +E+F RFV+ GEEEEKV +LREAFKMYEM+G G IT  SL+R L KLGE  ++++C  MI
Sbjct: 61  LEDFVRFVEGGEEEEKVNDLREAFKMYEMDGFGGITPKSLKRMLSKLGESKTIDECRMMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           A+FDL+GDG++SFDEF+ MM
Sbjct: 121 ARFDLNGDGIISFDEFRSMM 140

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022928328.18.5e-6897.16putative calcium-binding protein CML19 [Cucurbita moschata][more]
KYP32675.17.5e-4063.57Putative calcium-binding protein CML19 [Cajanus cajan][more]
XP_020208193.17.5e-4063.57putative calcium-binding protein CML19 [Cajanus cajan][more]
RDY13437.15.4e-3862.32putative calcium-binding protein CML19, partial [Mucuna pruriens][more]
XP_014508533.12.7e-3760.71putative calcium-binding protein CML19 [Vigna radiata var. radiata][more]
Match NameE-valueIdentityDescription
AT5G42380.15.0e-3053.33calmodulin like 37[more]
AT1G76650.18.3e-2546.76calmodulin-like 38[more]
AT1G76640.12.3e-2245.52Calcium-binding EF-hand family protein[more]
AT4G12860.11.5e-2142.22EF hand calcium-binding protein family[more]
AT3G50770.11.1e-1942.86calmodulin-like 41[more]
Match NameE-valueIdentityDescription
sp|Q8RYJ8|CML19_ORYSJ5.1e-3253.28Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9FIH9|CML37_ARATH9.1e-2953.33Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=2 SV=1[more]
sp|Q8RYJ9|CML23_ORYSJ6.5e-2748.25Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9SRE6|CML38_ARATH1.5e-2346.76Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=2 SV=1[more]
sp|Q9SRE7|CML39_ARATH4.1e-2145.52Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A151QQW8|A0A151QQW8_CAJCA5.0e-4063.57Putative calcium-binding protein CML19 OS=Cajanus cajan OX=3821 GN=KK1_046572 PE... [more]
tr|A0A1S3UR21|A0A1S3UR21_VIGRR1.8e-3760.71putative calcium-binding protein CML19 OS=Vigna radiata var. radiata OX=3916 GN=... [more]
tr|A0A2C9UHG0|A0A2C9UHG0_MANES1.8e-3764.93Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_15G159100 PE=4 SV=... [more]
tr|W9SWS7|W9SWS7_9ROSA3.9e-3760.00Calcium-binding protein CML38 OS=Morus notabilis OX=981085 GN=L484_017621 PE=4 S... [more]
tr|A0A2I4ERQ5|A0A2I4ERQ5_9ROSI1.1e-3658.57putative calcium-binding protein CML19 OS=Juglans regia OX=51240 GN=LOC108992079... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15572-RACarg15572-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 6..34
e-value: 0.0076
score: 25.5
coord: 115..141
e-value: 0.77
score: 18.7
coord: 79..107
e-value: 2.3
score: 14.8
coord: 43..71
e-value: 3.2
score: 13.7
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 92..140
e-value: 5.8E-12
score: 45.1
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 8..68
e-value: 3.1E-9
score: 37.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 111..141
score: 14.709
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 2..37
score: 12.561
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 75..110
score: 9.632
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 39..74
score: 11.278
IPR002048EF-hand domainCDDcd00051EFhcoord: 79..140
e-value: 9.11644E-13
score: 58.3281
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 75..141
e-value: 4.6E-20
score: 73.6
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..74
e-value: 3.5E-15
score: 58.1
NoneNo IPR availablePANTHERPTHR43996:SF5CALCIUM-BINDING PROTEIN CML40-RELATEDcoord: 1..140
NoneNo IPR availablePANTHERPTHR43996FAMILY NOT NAMEDcoord: 1..140
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 15..27
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 124..136
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 4..140

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg15572Cla004056Watermelon (97103) v1carwmB0360
Carg15572Cla019269Watermelon (97103) v1carwmB0363
Carg15572Csa3G588520Cucumber (Chinese Long) v2carcuB0395
Carg15572Csa1G002110Cucumber (Chinese Long) v2carcuB0394
Carg15572MELO3C026214Melon (DHL92) v3.5.1carmeB0371
Carg15572ClCG06G017800Watermelon (Charleston Gray)carwcgB0362
Carg15572ClCG05G013360Watermelon (Charleston Gray)carwcgB0361
Carg15572CSPI01G00620Wild cucumber (PI 183967)carcpiB0399
Carg15572CSPI03G25670Wild cucumber (PI 183967)carcpiB0400
Carg15572Cucsa.054260Cucumber (Gy14) v1carcgyB0425
Carg15572CmaCh11G014910Cucurbita maxima (Rimu)carcmaB0520
Carg15572CmaCh10G000700Cucurbita maxima (Rimu)carcmaB0518
Carg15572CmaCh11G000410Cucurbita maxima (Rimu)carcmaB0519
Carg15572CmoCh11G016280Cucurbita moschata (Rifu)carcmoB0502
Carg15572CmoCh11G000420Cucurbita moschata (Rifu)carcmoB0503
Carg15572Lsi06G016340Bottle gourd (USVL1VR-Ls)carlsiB335
Carg15572Lsi07G014660Bottle gourd (USVL1VR-Ls)carlsiB337
Carg15572Cp4.1LG04g16040Cucurbita pepo (Zucchini)carcpeB0520
Carg15572Cp4.1LG04g05890Cucurbita pepo (Zucchini)carcpeB0519
Carg15572Cp4.1LG18g08810Cucurbita pepo (Zucchini)carcpeB0516
Carg15572CsGy1G000630Cucumber (Gy14) v2carcgybB0374
Carg15572CsGy3G024090Cucumber (Gy14) v2carcgybB0375
Carg15572MELO3C032128.2Melon (DHL92) v3.6.1carmedB0378
Carg15572MELO3C026214.2Melon (DHL92) v3.6.1carmedB0376
Carg15572CsaV3_3G027200Cucumber (Chinese Long) v3carcucB0399
Carg15572CsaV3_1G000650Cucumber (Chinese Long) v3carcucB0398
Carg15572Cla97C05G094910Watermelon (97103) v2carwmbB0387
Carg15572Cla97C06G127530Watermelon (97103) v2carwmbB0388
Carg15572Bhi01G001594Wax gourdcarwgoB0453
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg15572Carg10289Silver-seed gourdcarcarB387
Carg15572Carg20175Silver-seed gourdcarcarB399
The following block(s) are covering this gene:
GeneOrganismBlock
Carg15572Silver-seed gourdcarcarB397
Carg15572Cucurbita maxima (Rimu)carcmaB0521
Carg15572Cucurbita moschata (Rifu)carcmoB0504
Carg15572Cucurbita pepo (Zucchini)carcpeB0518
Carg15572Melon (DHL92) v3.5.1carmeB0373