Carg01574 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg01574
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	thioredoxin-like
Location	Cucurbita_argyrosperma_scaffold_004 : 1737505 .. 1738797 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAAACATTAAAAAAAAAAAATATAAGTGAATCAAACGAACACAAACAGCTCTGTTTACCCATCTCCAATGGCACTCGATAACTGTGTTCAACTGGGCGCCGCCGCCGCCACCGCCGCCTCCATGTGCGCCACCACTCCCAGACTCAAATCTTATCCCTTTTGTTATAATCACCACCTAAACACAACCTTAACCCTTCGACGCAAATTGCCCATTTCAACTTCCGGAAACAAGTCCTTCTCCCCCTCTACAATCATTTCCACTTTCAGAAAATCCTTCTCCCTTGCGTGTCGAGCTCGTGGTGCCGTCGACGATGGTTACTCACCCGATCCAGATTACATTGATTCTTCACTGCTTTTGTGTTTGTGGGGTTTGCGAATTTGATGAGATTTTTGTTTCTTTGTGCAGTGCCGGAGGTGACAGAGTCGAGTTGGGACAGTTTGGTGGTTGGGAGTCAAAAGGCCGTGCTGGTGGAGTTTTGGGCGCCGTGGTGTGGACCTTGCAAGATCATTGAGCCTGTGATTAAGGATTTGGCGCAGGAATATGCAGGAAAGATTGTGTGTTTGAAGCTCAATACAGATCTCAGCCCAAATGTGGCCTCAAAATATGGTATTAGAAGCATTCCCACGGTTCTGTTCTTCAAGAATGGGGAGAAAAGAGAGAGCGTCATTGGTGCTGTGCCCAGATCCACCTTGGCCGCCACCATTGACAAGTATACAGAGGTTTAATTTTGAAGAATCCCCACATCATCGAACTCCATCAGAGATACCTTCCTATATTTTTCTTCTTCAGCTTGTAAAAATTGCAAACTTTTTATGGATCTCTTCAATTCGACAATTTTCTTTTTGTTCTTCATGTATTTGAATCGTTTTATGTTTGGTTTTTGAAGTCTATTCATAACCTTTTCGAATTCGCAACTATTTTTGTAGTTTCTTGTCACTAATGGTGATATTTCCTCTCTCTTCACTTGCATAATAACTCGAGCACGACGCGTCTGCTCTTTTATCTGGGAACATGATTCGCATATGAGAATAGGAAGTTTTTTGTGCAATTCTTTCGGGTTTGTTCGTTTCGACCTTAAGATTAAGCATACACAAGTCATTTCCATGATCAATAAATTATAAATCTCCTGATGCAAAATCATTCGTCAAATACCAACAAGAACAACAACAGGTTGTATCCCAAGACTAAAGAGGGGCAACCTTGTTTTGCCTTCCATGTGGGACTCAACCTTTTAGTTCCCCAAAACTGTAGGAGAACCACTTGATTTTGTATTTTTAGTACTTTCTCAAG

mRNA sequence

TAAAAACATTAAAAAAAAAAAATATAAGTGAATCAAACGAACACAAACAGCTCTGTTTACCCATCTCCAATGGCACTCGATAACTGTGTTCAACTGGGCGCCGCCGCCGCCACCGCCGCCTCCATGTGCGCCACCACTCCCAGACTCAAATCTTATCCCTTTTGTTATAATCACCACCTAAACACAACCTTAACCCTTCGACGCAAATTGCCCATTTCAACTTCCGGAAACAAGTCCTTCTCCCCCTCTACAATCATTTCCACTTTCAGAAAATCCTTCTCCCTTGCGTGTCGAGCTCGTGGTGCCGTCGACGATGTGCCGGAGGTGACAGAGTCGAGTTGGGACAGTTTGGTGGTTGGGAGTCAAAAGGCCGTGCTGGTGGAGTTTTGGGCGCCGTGGTGTGGACCTTGCAAGATCATTGAGCCTGTGATTAAGGATTTGGCGCAGGAATATGCAGGAAAGATTGTGTGTTTGAAGCTCAATACAGATCTCAGCCCAAATGTGGCCTCAAAATATGGTATTAGAAGCATTCCCACGGTTCTGTTCTTCAAGAATGGGGAGAAAAGAGAGAGCGTCATTGGTGCTGTGCCCAGATCCACCTTGGCCGCCACCATTGACAAGTATACAGAGGTTTAATTTTGAAGAATCCCCACATCATCGAACTCCATCAGAGATACCTTCCTATATTTTTCTTCTTCAGCTTGTAAAAATTGCAAACTTTTTATGGATCTCTTCAATTCGACAATTTTCTTTTTGTTCTTCATGTATTTGAATCGTTTTATGTTTGGTTTTTGAAGTCTATTCATAACCTTTTCGAATTCGCAACTATTTTTGTAGTTTCTTGTCACTAATGGTGATATTTCCTCTCTCTTCACTTGCATAATAACTCGAGCACGACGCGTCTGCTCTTTTATCTGGGAACATGATTCGCATATGAGAATAGGAAGTTTTTTGTGCAATTCTTTCGGGTTTGTTCGTTTCGACCTTAAGATTAAGCATACACAAGTCATTTCCATGATCAATAAATTATAAATCTCCTGATGCAAAATCATTCGTCAAATACCAACAAGAACAACAACAGGTTGTATCCCAAGACTAAAGAGGGGCAACCTTGTTTTGCCTTCCATGTGGGACTCAACCTTTTAGTTCCCCAAAACTGTAGGAGAACCACTTGATTTTGTATTTTTAGTACTTTCTCAAG

Coding sequence (CDS)

ATGGCACTCGATAACTGTGTTCAACTGGGCGCCGCCGCCGCCACCGCCGCCTCCATGTGCGCCACCACTCCCAGACTCAAATCTTATCCCTTTTGTTATAATCACCACCTAAACACAACCTTAACCCTTCGACGCAAATTGCCCATTTCAACTTCCGGAAACAAGTCCTTCTCCCCCTCTACAATCATTTCCACTTTCAGAAAATCCTTCTCCCTTGCGTGTCGAGCTCGTGGTGCCGTCGACGATGTGCCGGAGGTGACAGAGTCGAGTTGGGACAGTTTGGTGGTTGGGAGTCAAAAGGCCGTGCTGGTGGAGTTTTGGGCGCCGTGGTGTGGACCTTGCAAGATCATTGAGCCTGTGATTAAGGATTTGGCGCAGGAATATGCAGGAAAGATTGTGTGTTTGAAGCTCAATACAGATCTCAGCCCAAATGTGGCCTCAAAATATGGTATTAGAAGCATTCCCACGGTTCTGTTCTTCAAGAATGGGGAGAAAAGAGAGAGCGTCATTGGTGCTGTGCCCAGATCCACCTTGGCCGCCACCATTGACAAGTATACAGAGGTTTAA

Protein sequence

MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRRKLPISTSGNKSFSPSTIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAATIDKYTEV

Homology

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BLAST of Carg01574 vs. NCBI nr
Match: XP_023550329.1 (thioredoxin M-type, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 368.2 bits (944), Expect = 1.6e-98
Identity = 182/188 (96.81%), Postives = 184/188 (97.87%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRRKLPISTSGNKSFSPS 60
           MALDNCVQLG AAA  A+MCATTPRLKSYPFCYNHH NTTLTL+RKLPISTSGNKSFSPS
Sbjct: 1   MALDNCVQLGTAAAATAAMCATTPRLKSYPFCYNHHPNTTLTLQRKLPISTSGNKSFSPS 60

Query: 61  TIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPV 120
           TIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPV
Sbjct: 61  TIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPV 120

Query: 121 IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAA 180
           IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAA
Sbjct: 121 IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAA 180

Query: 181 TIDKYTEV 189
           TIDKYTEV
Sbjct: 181 TIDKYTEV 188

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BLAST of Carg01574 vs. NCBI nr
Match: XP_022939027.1 (thioredoxin M5, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 367.5 bits (942), Expect = 2.8e-98
Identity = 185/188 (98.40%), Postives = 185/188 (98.40%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRRKLPISTSGNKSFSPS 60
           MALDNCVQLG AAA AASMCATT RLKSYPFCYNHHLNTTLTLRRKLPISTSGNKSFSPS
Sbjct: 1   MALDNCVQLGTAAA-AASMCATTTRLKSYPFCYNHHLNTTLTLRRKLPISTSGNKSFSPS 60

Query: 61  TIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPV 120
           TIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPV
Sbjct: 61  TIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPV 120

Query: 121 IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAA 180
           IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAA
Sbjct: 121 IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAA 180

Query: 181 TIDKYTEV 189
           TIDKYTEV
Sbjct: 181 TIDKYTEV 187

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BLAST of Carg01574 vs. NCBI nr
Match: XP_022993934.1 (thioredoxin M-type, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 355.5 bits (911), Expect = 1.1e-94
Identity = 177/188 (94.15%), Postives = 181/188 (96.28%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRRKLPISTSGNKSFSPS 60
           MALDNCVQL  AAATA +MCATTPR+KS+PFCYNHHLNTTLTL+RKLPISTSGNKSFSPS
Sbjct: 1   MALDNCVQLSTAAATATAMCATTPRIKSHPFCYNHHLNTTLTLQRKLPISTSGNKSFSPS 60

Query: 61  TIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPV 120
           TIISTFRKSFSL CRARGAVDDVPEVTESSWDSLV GSQKAVLVEFWAPWCGPCKIIEPV
Sbjct: 61  TIISTFRKSFSL-CRARGAVDDVPEVTESSWDSLVFGSQKAVLVEFWAPWCGPCKIIEPV 120

Query: 121 IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAA 180
           IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESV GAVPRSTLAA
Sbjct: 121 IKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVSGAVPRSTLAA 180

Query: 181 TIDKYTEV 189
           TIDKY EV
Sbjct: 181 TIDKYAEV 187

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BLAST of Carg01574 vs. NCBI nr
Match: XP_022955939.1 (uncharacterized protein LOC111457772 [Cucurbita moschata])

HSP 1 Score: 267.3 bits (682), Expect = 3.9e-68
Identity = 137/190 (72.11%), Postives = 157/190 (82.63%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRR--KLPISTSGNKSFS 60
           MALDNC+QL    +TAA+M  TT   KS+PF    H   TL+L+R  K P ST+  KSFS
Sbjct: 1   MALDNCIQL----STAAAMYGTTSIPKSHPFSSTFHQKPTLSLQRLNKFPTSTARFKSFS 60

Query: 61  PSTIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIE 120
           PST +STF KS S+ C+ARGAVDDV EVTESSW+ LVV S+K VLVEFWAPWCGPCK+IE
Sbjct: 61  PSTSVSTFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIE 120

Query: 121 PVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTL 180
           PVI++LAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNG+KRE VIGAVP+STL
Sbjct: 121 PVIRELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTL 180

Query: 181 AATIDKYTEV 189
           +A+IDKY EV
Sbjct: 181 SASIDKYLEV 186

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BLAST of Carg01574 vs. NCBI nr
Match: XP_022989806.1 (uncharacterized protein LOC111486882 [Cucurbita maxima])

HSP 1 Score: 267.3 bits (682), Expect = 3.9e-68
Identity = 137/190 (72.11%), Postives = 157/190 (82.63%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRR--KLPISTSGNKSFS 60
           MALDNC+QL    +TAA+M  TT   KS+PF    H  TTL+L+R  K P S +  KSFS
Sbjct: 1   MALDNCIQL----STAAAMYGTTSIPKSHPFSSTFHQKTTLSLQRLNKFPTSIARFKSFS 60

Query: 61  PSTIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIE 120
           PST +STF KS S+ C+ARGAVDDV EVTESSW+ LVV S+K VLVEFWAPWCGPCK+IE
Sbjct: 61  PSTSVSTFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIE 120

Query: 121 PVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTL 180
           PVI++LAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNG+KRE VIGAVP+STL
Sbjct: 121 PVIRELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTL 180

Query: 181 AATIDKYTEV 189
           +A+IDKY EV
Sbjct: 181 SASIDKYLEV 186

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BLAST of Carg01574 vs. TAIR10
Match: AT4G03520.1 (Thioredoxin superfamily protein)

HSP 1 Score: 149.4 bits (376), Expect = 2.2e-36
Identity = 65/135 (48.15%), Postives = 96/135 (71.11%), Query Frame = 0


Query: 56  SFSPSTIISTFRKSFS-----LACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPW 115
           S SP+++ S  +   S     + C A+    D+  V +S+WDSLV+ +   V+V+FWAPW
Sbjct: 50  SLSPASLTSIHQPRVSRLRRAVVCEAQETTTDIQVVNDSTWDSLVLKATGPVVVDFWAPW 109

Query: 116 CGPCKIIEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVI 175
           CGPCK+I+P++ DLAQ Y GKI   KLNTD SPN   +YG+RSIPT++ F  GEK++++I
Sbjct: 110 CGPCKMIDPLVNDLAQHYTGKIKFYKLNTDESPNTPGQYGVRSIPTIMIFVGGEKKDTII 169

Query: 176 GAVPRSTLAATIDKY 186
           GAVP++TL +++DK+
Sbjct: 170 GAVPKTTLTSSLDKF 184

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BLAST of Carg01574 vs. TAIR10
Match: AT1G03680.1 (thioredoxin M-type 1)

HSP 1 Score: 145.2 bits (365), Expect = 4.1e-35
Identity = 62/130 (47.69%), Postives = 94/130 (72.31%), Query Frame = 0


Query: 56  SFSPSTIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCK 115
           S S ++ +S  R+   + C A+     +P V +S+WDSLV+ + + V V+FWAPWCGPCK
Sbjct: 51  SLSKNSRVSRLRR--GVICEAQDTATGIPVVNDSTWDSLVLKADEPVFVDFWAPWCGPCK 110

Query: 116 IIEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPR 175
           +I+P++ +LAQ+YAG+    KLNTD SP    +YG+RSIPT++ F NGEK++++IGAV +
Sbjct: 111 MIDPIVNELAQKYAGQFKFYKLNTDESPATPGQYGVRSIPTIMIFVNGEKKDTIIGAVSK 170

Query: 176 STLAATIDKY 186
            TLA +I+K+
Sbjct: 171 DTLATSINKF 178

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BLAST of Carg01574 vs. TAIR10
Match: AT3G15360.1 (thioredoxin M-type 4)

HSP 1 Score: 145.2 bits (365), Expect = 4.1e-35
Identity = 62/117 (52.99%), Postives = 88/117 (75.21%), Query Frame = 0


Query: 72  LACRAR---GAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPVIKDLAQEY 131
           +AC A+    A  +VP +++S W + V+ S   VLVEFWAPWCGPC++I P++  LA+++
Sbjct: 74  IACEAQDTTAAAVEVPNLSDSEWQTKVLESDVPVLVEFWAPWCGPCRMIHPIVDQLAKDF 133

Query: 132 AGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAATIDKY 186
           AGK    K+NTD SPN A++YGIRS+PTV+ FK GEK++S+IGAVPR TL  TI+++
Sbjct: 134 AGKFKFYKINTDESPNTANRYGIRSVPTVIIFKGGEKKDSIIGAVPRETLEKTIERF 190

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BLAST of Carg01574 vs. TAIR10
Match: AT2G15570.2 (Thioredoxin superfamily protein)

HSP 1 Score: 111.7 bits (278), Expect = 5.0e-25
Identity = 58/137 (42.34%), Postives = 87/137 (63.50%), Query Frame = 0


Query: 56  SFSPSTIISTFRKSF--SLACRARGA---VDD---VPEVTESSWDSLVVGSQKAVLVEFW 115
           SFSP ++  + R+S   S A R R +   V D     EVT+ SW+  V+ S+  VLVEF+
Sbjct: 34  SFSPRSLRFSDRRSLLSSSASRLRLSPLCVRDSRAAAEVTQRSWEDSVLKSETPVLVEFY 93

Query: 116 APWCGPCKIIEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRE 175
             WCGPC+++  +I ++A +YAGK+ C  LN D    VA +Y I+++P VL FKNGEKRE
Sbjct: 94  TSWCGPCRMVHRIIDEIAGDYAGKLNCYLLNADNDLPVAEEYEIKAVPVVLLFKNGEKRE 153

Query: 176 SVIGAVPRSTLAATIDK 185
           S++G +P+    + I++
Sbjct: 154 SIMGTMPKEFYISAIER 170

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BLAST of Carg01574 vs. TAIR10
Match: AT1G43560.1 (thioredoxin Y2)

HSP 1 Score: 85.9 bits (211), Expect = 2.9e-17
Identity = 36/102 (35.29%), Postives = 67/102 (65.69%), Query Frame = 0


Query: 87  TESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPVIKDLAQEYAGKIVCLKLNTDLSPNVA 146
           T +S+D L+  S K VLV+F+A WCGPC+++ P++ ++++     I  +K++T+  P++A
Sbjct: 64  TFNSFDDLLQNSDKPVLVDFYATWCGPCQLMVPILNEVSETLKDIIAVVKIDTEKYPSLA 123

Query: 147 SKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAATIDKYTEV 189
           +KY I ++PT + FK+G+  +   GA+P + L   I+   +V
Sbjct: 124 NKYQIEALPTFILFKDGKLWDRFEGALPANQLVERIENSLQV 165

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BLAST of Carg01574 vs. Swiss-Prot
Match: sp|Q9ZP20|TRXM5_ORYSJ (Thioredoxin M5, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TRXM PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 6.6e-43
Identity = 83/145 (57.24%), Postives = 105/145 (72.41%), Query Frame = 0


Query: 44  RRKLPISTSGNKSFSPSTII---STFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQK 103
           R +L +S +G+    P   +   S  R +   AC+    VD+V    E +WDS+V+GS+ 
Sbjct: 25  RDRLLLSGAGSSQSKPRLSVASPSPLRPASRFACQCSNVVDEVVVADEKNWDSMVLGSEA 84

Query: 104 AVLVEFWAPWCGPCKIIEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFF 163
            VLVEFWAPWCGPC++I PVI +LA+EY GKI C K+NTD SPN+A+ YGIRSIPTVL F
Sbjct: 85  PVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIATNYGIRSIPTVLMF 144

Query: 164 KNGEKRESVIGAVPRSTLAATIDKY 186
           KNGEK+ESVIGAVP++TLA  IDKY
Sbjct: 145 KNGEKKESVIGAVPKTTLATIIDKY 169

[Close]

BLAST of Carg01574 vs. Swiss-Prot
Match: sp|P07591|TRXM_SPIOL (Thioredoxin M-type, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 9.5e-42
Identity = 94/193 (48.70%), Postives = 126/193 (65.28%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLN--------TTLTLRRKLPISTS 60
           MA++NC+QL  +A+        T  +KS+     HHL         T   L+R  P  +S
Sbjct: 1   MAIENCLQLSTSASVG------TVAVKSHV----HHLQPSSKVNVPTFRGLKRSFPALSS 60

Query: 61  GNKSFSPSTIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCG 120
              S SP      FR S S+ C+A  AV +V +V +SSW   V+ S+  V+V+FWAPWCG
Sbjct: 61  SVSSSSP----RQFRYS-SVVCKASEAVKEVQDVNDSSWKEFVLESEVPVMVDFWAPWCG 120

Query: 121 PCKIIEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGA 180
           PCK+I PVI +LA+EY+GKI   KLNTD +P +A++Y IRSIPTVLFFKNGE++ES+IGA
Sbjct: 121 PCKLIAPVIDELAKEYSGKIAVYKLNTDEAPGIATQYNIRSIPTVLFFKNGERKESIIGA 178

Query: 181 VPRSTLAATIDKY 186
           VP+STL  +I+KY
Sbjct: 181 VPKSTLTDSIEKY 178

[Close]

BLAST of Carg01574 vs. Swiss-Prot
Match: sp|P48384|TRXM_PEA (Thioredoxin M-type, chloroplastic OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.2e-41
Identity = 75/110 (68.18%), Postives = 95/110 (86.36%), Query Frame = 0


Query: 76  ARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPVIKDLAQEYAGKIVCL 135
           AR AV++V  V +SSWD LV+GS+  VLV+FWAPWCGPC++I P+I +LA+EYAGKI C 
Sbjct: 62  AREAVNEVQVVNDSSWDELVIGSETPVLVDFWAPWCGPCRMIAPIIDELAKEYAGKIKCY 121

Query: 136 KLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAATIDKY 186
           KLNTD SPN A+KYGIRSIPTVLFFKNGE+++SVIGAVP++TL+  ++KY
Sbjct: 122 KLNTDESPNTATKYGIRSIPTVLFFKNGERKDSVIGAVPKATLSEKVEKY 171

[Close]

BLAST of Carg01574 vs. Swiss-Prot
Match: sp|Q41864|TRXM_MAIZE (Thioredoxin M-type, chloroplastic OS=Zea mays OX=4577 GN=TRM1 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.8e-40
Identity = 75/112 (66.96%), Postives = 90/112 (80.36%), Query Frame = 0


Query: 74  CRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPVIKDLAQEYAGKIV 133
           C+++ AVD+V    E +WD LV+  +  VLVEFWAPWCGPC++I PVI +LA++YAGKI 
Sbjct: 52  CQSKNAVDEVVVADEKNWDGLVMACETPVLVEFWAPWCGPCRMIAPVIDELAKDYAGKIT 111

Query: 134 CLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAATIDKY 186
           C K+NTD SPNVAS YGIRSIPTVL FK GEK+ESVIGAVP+STL   IDKY
Sbjct: 112 CCKVNTDDSPNVASTYGIRSIPTVLIFKGGEKKESVIGAVPKSTLTTLIDKY 163

[Close]

BLAST of Carg01574 vs. Swiss-Prot
Match: sp|Q9ZP21|TRXM_WHEAT (Thioredoxin M-type, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 7.6e-39
Identity = 70/119 (58.82%), Postives = 90/119 (75.63%), Query Frame = 0


Query: 67  RKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIEPVIKDLAQ 126
           R+     C+ +  VD+V    E +WD++V+  +  VLVEFWAPWCGPC++I PVI +LA+
Sbjct: 54  RRPSRFVCKCKNVVDEVIVADEKNWDNMVIACESPVLVEFWAPWCGPCRMIAPVIDELAK 113

Query: 127 EYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTLAATIDKY 186
           +Y GKI C K+NTD  PN+AS YGIRSIPTVL FK+GEK+ESVIGAVP++TL   IDKY
Sbjct: 114 DYVGKIKCCKVNTDDCPNIASTYGIRSIPTVLMFKDGEKKESVIGAVPKTTLCTIIDKY 172

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BLAST of Carg01574 vs. TrEMBL
Match: tr|A0A0A0KIC1|A0A0A0KIC1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G343710 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.6e-63
Identity = 135/192 (70.31%), Postives = 150/192 (78.12%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRRKLPISTSG----NKS 60
           MALDNC+QL    +TAA+MCA     KS      H+ ++T    +K   ST      NKS
Sbjct: 1   MALDNCIQL----STAATMCAAASIPKS------HYFSSTTNYPQKTSFSTLSLQRLNKS 60

Query: 61  FSPSTIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKI 120
            +P     TFRK FSL C+ARGAVDDV EVTESSW++LVV +QKAVLVEFWAPWCGPCKI
Sbjct: 61  PTP-----TFRKPFSLTCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKI 120

Query: 121 IEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRS 180
           IEPVIK+LA EYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVP+S
Sbjct: 121 IEPVIKELAAEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKS 177

Query: 181 TLAATIDKYTEV 189
           TL A+IDKY EV
Sbjct: 181 TLTASIDKYIEV 177

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BLAST of Carg01574 vs. TrEMBL
Match: tr|A0A1S4DTR8|A0A1S4DTR8_CUCME (thioredoxin-like OS=Cucumis melo OX=3656 GN=LOC107990489 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.7e-62
Identity = 133/192 (69.27%), Postives = 150/192 (78.12%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRRKLPISTSG----NKS 60
           MALDNC+QL    +TAA+MCA     KS      H+ ++T    +KL  ST      NK 
Sbjct: 1   MALDNCIQL----STAATMCAAASIPKS------HYFSSTANFPQKLSFSTLSLQRLNKF 60

Query: 61  FSPSTIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKI 120
            +P     TFRK FS+ C+ARGAVDDV EVTESSW++LVV +QKAVLVEFWAPWCGPCKI
Sbjct: 61  PTP-----TFRKPFSVTCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKI 120

Query: 121 IEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRS 180
           IEPVIK+LA EYAGKI CLKLNTD+SPNVASKYGIRSIPTVLFFKNGEKRESVIGAVP+S
Sbjct: 121 IEPVIKELAAEYAGKIECLKLNTDVSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKS 177

Query: 181 TLAATIDKYTEV 189
           TLAA+IDKY EV
Sbjct: 181 TLAASIDKYIEV 177

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BLAST of Carg01574 vs. TrEMBL
Match: tr|A0A061FPW8|A0A061FPW8_THECC (Thioredoxin M-type 4 OS=Theobroma cacao OX=3641 GN=TCM_035372 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.6e-49
Identity = 112/197 (56.85%), Postives = 140/197 (71.07%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSYPFCYNHHLNTTLTLRRKLPISTSGNK---SF 60
           MAL+NC QL +A  T AS+      L+SY     HH ++   +   LP     NK   SF
Sbjct: 1   MALENCFQLSSACTTRASV------LQSY-----HHFSSVEKVH--LPTFRGFNKPNLSF 60

Query: 61  SPSTIISTFRKSFS-------LACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPW 120
           + S+  S+   SF+       L C+AR AVD+V  VTESSW  LVVGS+  VLVEFWAPW
Sbjct: 61  TSSSSSSSLAHSFTRRSQKSRLICKAREAVDEVAVVTESSWGELVVGSETPVLVEFWAPW 120

Query: 121 CGPCKIIEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVI 180
           CGPC++IEPVI +LA+EYAGKI C KLNTD SPN+A+++GIRSIPT+LFFK+GEK+ES+I
Sbjct: 121 CGPCRMIEPVIAELAKEYAGKIACYKLNTDDSPNIATEFGIRSIPTMLFFKDGEKKESII 180

Query: 181 GAVPRSTLAATIDKYTE 188
           GAVP+STLAATIDKY +
Sbjct: 181 GAVPKSTLAATIDKYVD 184

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BLAST of Carg01574 vs. TrEMBL
Match: tr|A0A2I4DIN7|A0A2I4DIN7_9ROSI (thioredoxin M-type, chloroplastic-like OS=Juglans regia OX=51240 GN=LOC108980524 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.3e-48
Identity = 109/189 (57.67%), Postives = 136/189 (71.96%), Query Frame = 0


Query: 1   MALDNCVQLGAAAATAASMCATTPRLKSY-PFCYNHHLNTTLTLRRKLPISTSGNKSFS- 60
           MAL+NC Q+     T A+       L+ Y PF     L+  +    K  I TS   S S 
Sbjct: 1   MALENCFQVSTVCTTRAA------ALQCYHPFYSKEKLHLPICSGLKKSILTSSLSSSSY 60

Query: 61  PSTIISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCKIIE 120
           P ++ S  RKS S+ C+AR A+D+V  VT+SSW++LV+ S+  VLVEFWAPWCGPCK+I 
Sbjct: 61  PHSLKSRPRKS-SIVCKARQALDEVQVVTDSSWNNLVIASENPVLVEFWAPWCGPCKMIA 120

Query: 121 PVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPRSTL 180
           PVI +LA+EYAGKI+C KLNTD SPN+A+KYGIRSIPTVLFFKNGEK+ESVIGAVP+STL
Sbjct: 121 PVIDELAKEYAGKILCCKLNTDDSPNIATKYGIRSIPTVLFFKNGEKKESVIGAVPKSTL 180

Query: 181 AATIDKYTE 188
           + TI+KY E
Sbjct: 181 STTIEKYIE 182

[Close]

BLAST of Carg01574 vs. TrEMBL
Match: tr|A0A2P4HX44|A0A2P4HX44_QUESU (Thioredoxin m-type, chloroplastic OS=Quercus suber OX=58331 GN=CFP56_39833 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.0e-48
Identity = 106/192 (55.21%), Postives = 134/192 (69.79%), Query Frame = 0


Query: 1   MALDNCVQLGAAAA--TAASMCATTPRLKSYPFCYNHHLNTTLTLRRKLPISTSGNKSFS 60
           MAL+NC QL       TAA  C        +PF     LN       K  + TS   S S
Sbjct: 1   MALENCFQLSTVCTTRTAALQC-------YHPFSSKERLNFLTCNALKKSLLTSATCSSS 60

Query: 61  PSTI---ISTFRKSFSLACRARGAVDDVPEVTESSWDSLVVGSQKAVLVEFWAPWCGPCK 120
            S+    IS   +  S+ C+AR AVD+V  VT+S+W++LV+ S+  VLVEFWAPWCGPC+
Sbjct: 61  SSSYPHSISVKPRKPSIVCKAREAVDEVQVVTDSNWNNLVIASENPVLVEFWAPWCGPCR 120

Query: 121 IIEPVIKDLAQEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPR 180
           +I PVI++LA+EYAGKI+CLKLNTD  PN+A++YGIRSIPTVLFFKNGEK+ESVIGAVP+
Sbjct: 121 MIAPVIEELAKEYAGKILCLKLNTDDCPNIATQYGIRSIPTVLFFKNGEKKESVIGAVPK 180

Query: 181 STLAATIDKYTE 188
           STL+AT++KY E
Sbjct: 181 STLSATVEKYVE 185

The following BLAST results are available for this feature:

BLAST of Carg01574 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023550329.1	1.6e-98	96.81	thioredoxin M-type, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022939027.1	2.8e-98	98.40	thioredoxin M5, chloroplastic-like [Cucurbita moschata]	[more]
XP_022993934.1	1.1e-94	94.15	thioredoxin M-type, chloroplastic-like [Cucurbita maxima]	[more]
XP_022955939.1	3.9e-68	72.11	uncharacterized protein LOC111457772 [Cucurbita moschata]	[more]
XP_022989806.1	3.9e-68	72.11	uncharacterized protein LOC111486882 [Cucurbita maxima]	[more]

BLAST of Carg01574 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G03520.1	2.2e-36	48.15	Thioredoxin superfamily protein	[more]
AT1G03680.1	4.1e-35	47.69	thioredoxin M-type 1	[more]
AT3G15360.1	4.1e-35	52.99	thioredoxin M-type 4	[more]
AT2G15570.2	5.0e-25	42.34	Thioredoxin superfamily protein	[more]
AT1G43560.1	2.9e-17	35.29	thioredoxin Y2	[more]

BLAST of Carg01574 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9ZP20\|TRXM5_ORYSJ	6.6e-43	57.24	Thioredoxin M5, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TRXM P...	[more]
sp\|P07591\|TRXM_SPIOL	9.5e-42	48.70	Thioredoxin M-type, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2	[more]
sp\|P48384\|TRXM_PEA	1.2e-41	68.18	Thioredoxin M-type, chloroplastic OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
sp\|Q41864\|TRXM_MAIZE	1.8e-40	66.96	Thioredoxin M-type, chloroplastic OS=Zea mays OX=4577 GN=TRM1 PE=2 SV=1	[more]
sp\|Q9ZP21\|TRXM_WHEAT	7.6e-39	58.82	Thioredoxin M-type, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1	[more]

BLAST of Carg01574 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KIC1\|A0A0A0KIC1_CUCSA	5.6e-63	70.31	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G343710 PE=4 SV=1	[more]
tr\|A0A1S4DTR8\|A0A1S4DTR8_CUCME	4.7e-62	69.27	thioredoxin-like OS=Cucumis melo OX=3656 GN=LOC107990489 PE=4 SV=1	[more]
tr\|A0A061FPW8\|A0A061FPW8_THECC	1.6e-49	56.85	Thioredoxin M-type 4 OS=Theobroma cacao OX=3641 GN=TCM_035372 PE=4 SV=1	[more]
tr\|A0A2I4DIN7\|A0A2I4DIN7_9ROSI	1.3e-48	57.67	thioredoxin M-type, chloroplastic-like OS=Juglans regia OX=51240 GN=LOC108980524...	[more]
tr\|A0A2P4HX44\|A0A2P4HX44_QUESU	3.0e-48	55.21	Thioredoxin m-type, chloroplastic OS=Quercus suber OX=58331 GN=CFP56_39833 PE=4 ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0045454	cell redox homeostasis
GO:0006662	glycerol ether metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0015035	protein disulfide oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036249	Thioredoxin-like_sf
IPR017937	Thioredoxin_CS
IPR013766	Thioredoxin_domain
IPR005746	Thioredoxin

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045454	cell redox homeostasis
biological_process	GO:0006662	glycerol ether metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0015035	protein disulfide oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01574-RA	Carg01574-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00421	THIOREDOXIN	coord: 110..119 score: 63.28 coord: 102..110 score: 64.57 coord: 150..161 score: 44.63
None	No IPR available	GENE3D	G3DSA:3.40.30.10		coord: 83..187 e-value: 1.8E-33 score: 116.7
None	No IPR available	PANTHER	PTHR10438:SF367	SUBFAMILY NOT NAMED	coord: 5..187
None	No IPR available	CDD	cd02947	TRX_family	coord: 89..183 e-value: 7.24332E-36 score: 121.511
IPR005746	Thioredoxin	TIGRFAM	TIGR01068	TIGR01068	coord: 87..184 e-value: 6.4E-35 score: 117.4
IPR005746	Thioredoxin	PANTHER	PTHR10438	THIOREDOXIN	coord: 5..187
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 83..184 e-value: 9.1E-32 score: 109.0
IPR013766	Thioredoxin domain	PROSITE	PS51352	THIOREDOXIN_2	coord: 61..187 score: 14.659
IPR017937	Thioredoxin, conserved site	PROSITE	PS00194	THIOREDOXIN_1	coord: 103..121
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	SSF52833	Thioredoxin-like	coord: 72..186

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg01574	Cla013870	Watermelon (97103) v1	carwmB0824
Carg01574	Csa6G343710	Cucumber (Chinese Long) v2	carcuB0852
Carg01574	MELO3C008421	Melon (DHL92) v3.5.1	carmeB0824
Carg01574	ClCG08G006360	Watermelon (Charleston Gray)	carwcgB0803
Carg01574	CSPI06G18180	Wild cucumber (PI 183967)	carcpiB0872
Carg01574	Cucsa.077150	Cucumber (Gy14) v1	carcgyB0944
Carg01574	CmaCh04G024160	Cucurbita maxima (Rimu)	carcmaB1150
Carg01574	CmaCh15G006320	Cucurbita maxima (Rimu)	carcmaB1148
Carg01574	CmoCh15G006400	Cucurbita moschata (Rifu)	carcmoB1118
Carg01574	CmoCh04G025340	Cucurbita moschata (Rifu)	carcmoB1121
Carg01574	Lsi08G005140	Bottle gourd (USVL1VR-Ls)	carlsiB758
Carg01574	Cp4.1LG01g20890	Cucurbita pepo (Zucchini)	carcpeB1158
Carg01574	CsGy6G017640	Cucumber (Gy14) v2	carcgybB0826
Carg01574	MELO3C008421.2	Melon (DHL92) v3.6.1	carmedB0835
Carg01574	CsaV3_6G031060	Cucumber (Chinese Long) v3	carcucB0876
Carg01574	Cla97C08G149150	Watermelon (97103) v2	carwmbB0862
Carg01574	Bhi04G001429	Wax gourd	carwgoB0967

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg01574	Carg15765	Silver-seed gourd	carcarB076

The following block(s) are covering this gene:


Gene	Organism	Block
Carg01574	Cucurbita maxima (Rimu)	carcmaB1146
Carg01574	Cucurbita moschata (Rifu)	carcmoB1116
Carg01574	Cucurbita pepo (Zucchini)	carcpeB1157
Carg01574	Cucurbita pepo (Zucchini)	carcpeB1161