Carg00021 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg00021
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Metallothionein-like protein
Location	Cucurbita_argyrosperma_scaffold_001 : 83814 .. 85285 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAGAGATCATAAGCCAAAAAAAGTTCCATAAGCTATCAGTTTTTCGTTCTCCTACAACTTCTCTAGCTTCTTCACCATGTCAGGCAAGTGCGGCAACTGCGACTGCTCCGACAGCAGCAACTGCGTGTAAGTATTTTTTTTTTTTTTTTTTTTTGGTTTTCCTACGAACTTTGCAGCAAATCTCATCAAGAGCTTGCTTGTCTCAAAGCTATGATTCTGTTGCCCTCTCAGAAATTGTCTTAATATAGCAAGTCTGTCAATATGGTCCTTGGTTGCCCTCCCAATTATGACAGTTTGTTTGATGCTATAAAGGGAAGCAGATGGGGCAATAAACTATATTGGGGTGAAACTAAAAGTTCGAATCCAAATTATAGTGTCGCTGCAAATAAGATAATCATAAAACCACTTCTTCTTTCTTTTCCTTCACAATAAGACCAAATCAAAAGTAAGAAATTACTAGTAAAAAAAAAAAACGGATCGAAGAGTTTGCACATTCGAATTCATTCCTATGTTAATGACGTGCTCTATAATGACAGGAAAAAAGGGAACAGCTATGGCGCTGTCATCATGGAGAATGAGAAGAGGTAATTTTTTCAACTCATCGTGCATATACTTTCTTGAAACCAGCTGATTTAGAGCCCATTTTTTTTCATGAAAAATCAAATTCAAAGGATATAAACGCATTGAGAACTTTAATGAATCATGAACAAAAGTATGTTTAGATTTAGGAAAAAAGAGTGGAGCAGAGACAAAAAATCTCAACAAGTTAATGACTAAAAGTGCTTGACAGTACCTGGCATCTAACAATGAATCTCTTGGTTCTGCAGCTACTTTGACGCCATAGACATGGACGTCCCAGCGGCCGAGCACAATGACAAGTGCAGCTGCAACCCCTGCAGTTGCAAGGACTGCCACTGCGGCAAATGAACACATACACCTGTTGTTTCCATTAACACAGTTATATAAAGCCAGGAATAATGTAGCTGTGTTTGAAATGTGTCCTATTATGTTCAAAACCTCTGTGCTTAGCTAGAGGTGTGTTACGAATAATATCTACTTCTGTTCTTCATCCATAGTCATGTAATGCTGGTCACTTCTGTGGCCATGGGTTAACTATTAATGGATAAGTTATATACTAGTACACTCTCCCTCTCCTTCCATTTTTATTGTGGTTCTTTTCATCCTTGATTCAATTTGACTACAAATTACAAAGAGGCAACAATCTAGAAGGGCTTCAATACTTTTAAGAGTAGTGTAATACAATAAATCTATAGAACTCCACAAATCGAATAGAACAATTTCCTCAAAATAGTAAACCTGGCAAACAATTGATTCAAATCAAGCCCAGAATACATAGTGCATCACAGACCACAGTATCTTCTTGACCATTTTTTTAATTCTCTTTTCTTAGGTAGGAAACCGTATCTTATCAACAACAACTCATTAATAACATCCAAAGACATGTCAATTG

mRNA sequence

TTAGAGATCATAAGCCAAAAAAAGTTCCATAAGCTATCAGTTTTTCGTTCTCCTACAACTTCTCTAGCTTCTTCACCATGTCAGGCAAGTGCGGCAACTGCGACTGCTCCGACAGCAGCAACTGCGTGAAAAAAGGGAACAGCTATGGCGCTGTCATCATGGAGAATGAGAAGAGCTACTTTGACGCCATAGACATGGACGTCCCAGCGGCCGAGCACAATGACAAGTGCAGCTGCAACCCCTGCAGTTGCAAGGACTGCCACTGCGGCAAATGAACACATACACCTGTTGTTTCCATTAACACAGTTATATAAAGCCAGGAATAATGTAGCTGTGTTTGAAATGTGTCCTATTATGTTCAAAACCTCTGTGCTTAGCTAGAGGTGTGTTACGAATAATATCTACTTCTGTTCTTCATCCATAGTCATGTAATGCTGGTCACTTCTGTGGCCATGGGTTAACTATTAATGGATAAGTTATATACTAGTACACTCTCCCTCTCCTTCCATTTTTATTGTGGTTCTTTTCATCCTTGATTCAATTTGACTACAAATTACAAAGAGGCAACAATCTAGAAGGGCTTCAATACTTTTAAGAGTAGTGTAATACAATAAATCTATAGAACTCCACAAATCGAATAGAACAATTTCCTCAAAATAGTAAACCTGGCAAACAATTGATTCAAATCAAGCCCAGAATACATAGTGCATCACAGACCACAGTATCTTCTTGACCATTTTTTTAATTCTCTTTTCTTAGGTAGGAAACCGTATCTTATCAACAACAACTCATTAATAACATCCAAAGACATGTCAATTG

Coding sequence (CDS)

ATGTCAGGCAAGTGCGGCAACTGCGACTGCTCCGACAGCAGCAACTGCGTGAAAAAAGGGAACAGCTATGGCGCTGTCATCATGGAGAATGAGAAGAGCTACTTTGACGCCATAGACATGGACGTCCCAGCGGCCGAGCACAATGACAAGTGCAGCTGCAACCCCTGCAGTTGCAAGGACTGCCACTGCGGCAAATGA

Protein sequence

MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSCNPCSCKDCHCGK

Homology

[Close]

BLAST of Carg00021 vs. NCBI nr
Match: ADN33916.1 (metallothionein-like protein [Cucumis melo subsp. melo])

HSP 1 Score: 93.2 bits (230), Expect = 3.5e-16
Identity = 42/50 (84.00%), Postives = 44/50 (88.00%), Query Frame = 0


Query: 5  CGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSCN 55
          CGNCDCSD + CVKKGNSYGAVIMENE S FDAI M+ PAAEHNDKCSCN
Sbjct: 4  CGNCDCSDKTQCVKKGNSYGAVIMENENSSFDAIVMNFPAAEHNDKCSCN 53

[Close]

BLAST of Carg00021 vs. NCBI nr
Match: ACZ69388.1 (metallothionein-like protein type 3 [Cucumis sativus] >KGN56243.1 hypothetical protein Csa_3G105950 [Cucumis sativus])

HSP 1 Score: 89.4 bits (220), Expect = 5.0e-15
Identity = 40/50 (80.00%), Postives = 43/50 (86.00%), Query Frame = 0


Query: 5  CGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSCN 55
          CGNCDCSD + CVK+G+SYGAVI ENE S FDAI MD PAAEHNDKCSCN
Sbjct: 4  CGNCDCSDKTQCVKQGSSYGAVITENENSSFDAIVMDFPAAEHNDKCSCN 53

[Close]

BLAST of Carg00021 vs. NCBI nr
Match: PON68011.1 (Metallothionein-like protein type [Parasponia andersonii])

HSP 1 Score: 84.3 bits (207), Expect = 1.6e-13
Identity = 37/53 (69.81%), Postives = 42/53 (79.25%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCG+CDC+D S CVKKG+SY  VI+E EKSY DA+ MD PAAEHN KC C
Sbjct: 1  MSDKCGSCDCADKSQCVKKGSSYSLVIVETEKSYMDAVVMDAPAAEHNGKCKC 53

[Close]

BLAST of Carg00021 vs. NCBI nr
Match: PON91165.1 (Metallothionein-like protein type [Trema orientalis])

HSP 1 Score: 82.4 bits (202), Expect = 6.2e-13
Identity = 36/53 (67.92%), Postives = 42/53 (79.25%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCG+C+C+D S CVKKG+SY  VI+E EKSY DA+ MD PAAEHN KC C
Sbjct: 1  MSDKCGSCNCADKSQCVKKGSSYSLVIVETEKSYMDAVVMDAPAAEHNGKCKC 53

[Close]

BLAST of Carg00021 vs. NCBI nr
Match: POE77317.1 (metallothionein-like protein type 3 [Quercus suber])

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-12
Identity = 35/53 (66.04%), Postives = 42/53 (79.25%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCGNCDC+D S CVKKG+SYG VI+E +KSY + + MD PAAEH+ KC C
Sbjct: 1  MSDKCGNCDCADKSQCVKKGSSYGLVIVETDKSYSNTVVMDAPAAEHDGKCKC 53

[Close]

BLAST of Carg00021 vs. TAIR10
Match: AT3G15353.1 (metallothionein 3)

HSP 1 Score: 54.7 bits (130), Expect = 2.5e-08
Identity = 25/47 (53.19%), Postives = 34/47 (72.34%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEH 48
          MS  CG+CDC+D + CVKKG SY   I+E ++SY +A+ MDV A E+
Sbjct: 1  MSSNCGSCDCADKTQCVKKGTSYTFDIVETQESYKEAMIMDVGAEEN 47

[Close]

BLAST of Carg00021 vs. Swiss-Prot
Match: sp|O24059|MT3_MALDO (Metallothionein-like protein type 3 OS=Malus domestica OX=3750 GN=MT2 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.9e-13
Identity = 33/53 (62.26%), Postives = 39/53 (73.58%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MSGKC NCDC+DS+ CVKKGNSY  VI+E E    D + +D PAAEH+ KC C
Sbjct: 1  MSGKCDNCDCADSTQCVKKGNSYDLVIVETENRSMDTVFVDAPAAEHDGKCKC 53

[Close]

BLAST of Carg00021 vs. Swiss-Prot
Match: sp|Q40256|MT3_MUSAC (Metallothionein-like protein type 3 OS=Musa acuminata OX=4641 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.0e-11
Identity = 31/49 (63.27%), Postives = 35/49 (71.43%), Query Frame = 0


Query: 5  CGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          CGNCDC D S CVKKGNSYG  I+E EKSY D + +   AAEH+ KC C
Sbjct: 4  CGNCDCVDKSQCVKKGNSYGIDIVETEKSYVDEVIVAAEAAEHDGKCKC 52

[Close]

BLAST of Carg00021 vs. Swiss-Prot
Match: sp|P43389|MT3_ACTDE (Metallothionein-like protein type 3 OS=Actinidia deliciosa OX=3627 GN=pKIWI503 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.9e-11
Identity = 33/53 (62.26%), Postives = 38/53 (71.70%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCGNCDC+DSS CVKKGNS    I+E +KSY + + M VPAAE   KC C
Sbjct: 1  MSDKCGNCDCADSSQCVKKGNSID--IVETDKSYIEDVVMGVPAAESGGKCKC 51

[Close]

BLAST of Carg00021 vs. Swiss-Prot
Match: sp|A2WLS0|MT3A_ORYSI (Metallothionein-like protein 3A OS=Oryza sativa subsp. indica OX=39946 GN=MT3A PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.1e-10
Identity = 32/53 (60.38%), Postives = 37/53 (69.81%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCGNCDC+D S CVKKG SYG VI+E EKS+F+    +V A E N  C C
Sbjct: 1  MSDKCGNCDCADKSQCVKKGTSYGVVIVEAEKSHFE----EVAAGEENGGCKC 49

[Close]

BLAST of Carg00021 vs. Swiss-Prot
Match: sp|A1YTM8|MT3A_ORYSJ (Metallothionein-like protein 3A OS=Oryza sativa subsp. japonica OX=39947 GN=MT3A PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.1e-10
Identity = 32/53 (60.38%), Postives = 37/53 (69.81%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCGNCDC+D S CVKKG SYG VI+E EKS+F+    +V A E N  C C
Sbjct: 1  MSDKCGNCDCADKSQCVKKGTSYGVVIVEAEKSHFE----EVAAGEENGGCKC 49

[Close]

BLAST of Carg00021 vs. TrEMBL
Match: tr|E5GBS4|E5GBS4_CUCME (Metallothionein-like protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.3e-16
Identity = 42/50 (84.00%), Postives = 44/50 (88.00%), Query Frame = 0


Query: 5  CGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSCN 55
          CGNCDCSD + CVKKGNSYGAVIMENE S FDAI M+ PAAEHNDKCSCN
Sbjct: 4  CGNCDCSDKTQCVKKGNSYGAVIMENENSSFDAIVMNFPAAEHNDKCSCN 53

[Close]

BLAST of Carg00021 vs. TrEMBL
Match: tr|D2J0B0|D2J0B0_CUCSA (Metallothionein-like protein type 3 OS=Cucumis sativus OX=3659 GN=MTP-3 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.3e-15
Identity = 40/50 (80.00%), Postives = 43/50 (86.00%), Query Frame = 0


Query: 5  CGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSCN 55
          CGNCDCSD + CVK+G+SYGAVI ENE S FDAI MD PAAEHNDKCSCN
Sbjct: 4  CGNCDCSDKTQCVKQGSSYGAVITENENSSFDAIVMDFPAAEHNDKCSCN 53

[Close]

BLAST of Carg00021 vs. TrEMBL
Match: tr|A0A2P5D400|A0A2P5D400_PARAD (Metallothionein-like protein type OS=Parasponia andersonii OX=3476 GN=PanWU01x14_098150 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-13
Identity = 37/53 (69.81%), Postives = 42/53 (79.25%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCG+CDC+D S CVKKG+SY  VI+E EKSY DA+ MD PAAEHN KC C
Sbjct: 1  MSDKCGSCDCADKSQCVKKGSSYSLVIVETEKSYMDAVVMDAPAAEHNGKCKC 53

[Close]

BLAST of Carg00021 vs. TrEMBL
Match: tr|A0A2P5F066|A0A2P5F066_9ROSA (Metallothionein-like protein type OS=Trema orientalis OX=63057 GN=TorRG33x02_129810 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.1e-13
Identity = 36/53 (67.92%), Postives = 42/53 (79.25%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCG+C+C+D S CVKKG+SY  VI+E EKSY DA+ MD PAAEHN KC C
Sbjct: 1  MSDKCGSCNCADKSQCVKKGSSYSLVIVETEKSYMDAVVMDAPAAEHNGKCKC 53

[Close]

BLAST of Carg00021 vs. TrEMBL
Match: tr|A0A2P4J976|A0A2P4J976_QUESU (Metallothionein-like protein type 3 OS=Quercus suber OX=58331 GN=CFP56_45219 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 9.1e-13
Identity = 35/53 (66.04%), Postives = 42/53 (79.25%), Query Frame = 0


Query: 1  MSGKCGNCDCSDSSNCVKKGNSYGAVIMENEKSYFDAIDMDVPAAEHNDKCSC 54
          MS KCGNCDC+D S CVKKG+SYG VI+E +KSY + + MD PAAEH+ KC C
Sbjct: 1  MSDKCGNCDCADKSQCVKKGSSYGLVIVETDKSYSNTVVMDAPAAEHDGKCKC 53

The following BLAST results are available for this feature:

BLAST of Carg00021 vs. NCBI nr


Match Name	E-value	Identity	Description
ADN33916.1	3.5e-16	84.00	metallothionein-like protein [Cucumis melo subsp. melo]	[more]
ACZ69388.1	5.0e-15	80.00	metallothionein-like protein type 3 [Cucumis sativus] >KGN56243.1 hypothetical p...	[more]
PON68011.1	1.6e-13	69.81	Metallothionein-like protein type [Parasponia andersonii]	[more]
PON91165.1	6.2e-13	67.92	Metallothionein-like protein type [Trema orientalis]	[more]
POE77317.1	1.4e-12	66.04	metallothionein-like protein type 3 [Quercus suber]	[more]

BLAST of Carg00021 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G15353.1	2.5e-08	53.19	metallothionein 3	[more]

BLAST of Carg00021 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O24059\|MT3_MALDO	1.9e-13	62.26	Metallothionein-like protein type 3 OS=Malus domestica OX=3750 GN=MT2 PE=3 SV=1	[more]
sp\|Q40256\|MT3_MUSAC	1.0e-11	63.27	Metallothionein-like protein type 3 OS=Musa acuminata OX=4641 PE=3 SV=1	[more]
sp\|P43389\|MT3_ACTDE	3.9e-11	62.26	Metallothionein-like protein type 3 OS=Actinidia deliciosa OX=3627 GN=pKIWI503 P...	[more]
sp\|A2WLS0\|MT3A_ORYSI	1.1e-10	60.38	Metallothionein-like protein 3A OS=Oryza sativa subsp. indica OX=39946 GN=MT3A P...	[more]
sp\|A1YTM8\|MT3A_ORYSJ	1.1e-10	60.38	Metallothionein-like protein 3A OS=Oryza sativa subsp. japonica OX=39947 GN=MT3A...	[more]

BLAST of Carg00021 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|E5GBS4\|E5GBS4_CUCME	2.3e-16	84.00	Metallothionein-like protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
tr\|D2J0B0\|D2J0B0_CUCSA	3.3e-15	80.00	Metallothionein-like protein type 3 OS=Cucumis sativus OX=3659 GN=MTP-3 PE=2 SV=...	[more]
tr\|A0A2P5D400\|A0A2P5D400_PARAD	1.1e-13	69.81	Metallothionein-like protein type OS=Parasponia andersonii OX=3476 GN=PanWU01x14...	[more]
tr\|A0A2P5F066\|A0A2P5F066_9ROSA	4.1e-13	67.92	Metallothionein-like protein type OS=Trema orientalis OX=63057 GN=TorRG33x02_129...	[more]
tr\|A0A2P4J976\|A0A2P4J976_QUESU	9.1e-13	66.04	Metallothionein-like protein type 3 OS=Quercus suber OX=58331 GN=CFP56_45219 PE=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006878	cellular copper ion homeostasis

Vocabulary: **INTERPRO**
Term	Definition
IPR038918	MT3

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006878	cellular copper ion homeostasis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00021-RA	Carg00021-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038918	Metallothionein-like protein 3	PANTHER	PTHR33357	FAMILY NOT NAMED	coord: 2..58

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg00021	Csa3G105950	Cucumber (Chinese Long) v2	carcuB0684
Carg00021	MELO3C016513	Melon (DHL92) v3.5.1	carmeB0670
Carg00021	ClCG10G006940	Watermelon (Charleston Gray)	carwcgB0641
Carg00021	CSPI03G06180	Wild cucumber (PI 183967)	carcpiB0696
Carg00021	Cucsa.074770	Cucumber (Gy14) v1	carcgyB0740
Carg00021	CmaCh04G009030	Cucurbita maxima (Rimu)	carcmaB0948
Carg00021	CmoCh04G009700	Cucurbita moschata (Rifu)	carcmoB0923
Carg00021	Lsi05G007510	Bottle gourd (USVL1VR-Ls)	carlsiB614
Carg00021	Cp4.1LG01g08450	Cucurbita pepo (Zucchini)	carcpeB0938
Carg00021	CsGy3G006280	Cucumber (Gy14) v2	carcgybB0659
Carg00021	MELO3C016513.2	Melon (DHL92) v3.6.1	carmedB0683
Carg00021	CsaV3_3G006310	Cucumber (Chinese Long) v3	carcucB0698

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg00021	Watermelon (97103) v1	carwmB0664
Carg00021	Watermelon (97103) v2	carwmbB0686
Carg00021	Wax gourd	carwgoB0775
Carg00021	Wax gourd	carwgoB0778
Carg00021	Wax gourd	carwgoB0779
Carg00021	Silver-seed gourd	carcarB468
Carg00021	Cucurbita maxima (Rimu)	carcmaB0946
Carg00021	Cucurbita moschata (Rifu)	carcmoB0920
Carg00021	Cucurbita pepo (Zucchini)	carcpeB0937
Carg00021	Bottle gourd (USVL1VR-Ls)	carlsiB616