CSPI01G00280 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI01G00280
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionMitochondrial import inner membrane translocase subunit Tim8
LocationChr1 : 156011 .. 157692 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCTCGTCTCTTAACTCCCCGGAGCTGATGAATTTTATCAATGTGAGTTCATTTTCGTGGTTTTTAATTGTCACTTCTTGGCTATGATTCATTCTGGATCGAATATAAAAAATGAAATGACGGTAATCATTTGTGGTATTTGAAATAGGAAAAAAGACAAGATCTTTAATTTCCTCTCCCATGAATATGCTATTGGGTTGCTTTTTATCGCCTTTATGCATTACCTATTTAGTTCGTAGAATATCCGAGAATGGTAAAATTGATATCAGTCATGATTATTTCTTGTCAACTCTGCTACTTAGTGTCTAATAGGACATAATGTGTTTGCAAGAAACTATTTTTTAGAAGCAATGACAGAGCCCTTGAGACTGAGACCTACACTGACCCTGTCTAAAGATAAGTCTTAAGAAATGCTTTGTTGTCATCAGAACTCCTTACTCACCAGATAGTACGCTCTTGAAATTAGAATATTATGTCTACAGCAGATTTGAGTTTGTCTCTAAATCGGAAATGAATTGTATTTTTATCACAGTTTGACTTTGGTCCGAAAACTTCAGAGACGTCACTTGATGATTGTACTGCTATGTGGAATTTCAATGAATACAAATCTGGAAACAATACATGCTCTGACTTCTTTTTGTTTCTTTTTCTTGAAAACAGAAACAAACATTTTTTTCCGATAAGCGTATTCAATGAGTTTATCAGCCATGCAAGTAATTATAGGCTATGGAATTCCATGGGTTCATATCACTTAAAAAGATCAGAGTTTGTCTGAACTTGGGGGTTACAGTTATATGTCTTTAAGTATGATTTTGTTTATGTAAGATGTTCAATTTTATTTGCCAACAAACCATAGGTGGCAATCAGTTGACTACTCATTGTGTGTTGTCTACTCTTCACCACGCACCCTCCATCGTTTTTCATTTCTGGATTTCAACCTTCCTCCCTGTTCAGTTTAGACAGAAGATGCTTTGTTTTGTGACTTCTATTTGTTGCAGTGACAAAATTTAAATGCTATTGGTCAATTGTTTGGTCATTTGTTAAACAGGAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCTAAGCTTACCAGTGTCTGTTGGGACAAGTGCATAACTGGAACACCAGGGAGTAAGTTCAGCTCCAGTGAATCCAACTGTCTATCCAACTGTGCCCAGCGCTATATGGATATGAGCATCATCATTATGAAACGATTTCAAAATAGTTAAAAGGATCTCAACGTGAGGCTTGCTTTTCCAGTTACAAGACAATGTATTTTCTTGGGTATATTGTTCAATTCAAACCGATAGGCAAAAGGTTATGAATTTCTGGAAATACTGTTCATTTTTTTGAAGTTGTTGCTGCATTCCCTAAATCAGATTATTTTACATTACATCTTGATGAGAAGAGTAATGAACTGATATGGGGTATTCTGTTTCTTTCAGAGAAAAAAGAGAACGAAAATTAGGGGCATTCTGTTTTTTCTTTTTAATTTTGGCATATCAATATGTAGTTTGGAGTAATTGGAATGTGCTTTCGTTCGGGCAACCTGCTGCCTACTTCTTTGTCGTCCCAAGAGGAAGTCGCTCAACGTGGAGCACTGCCTCATATGTGTGGATGAAGATAGGCAGCAGGCGTTCCAATTTTCATGTAAGTGGCTACATGGAATAG

mRNA sequence

ATGGATTCCTCGTCTCTTAACTCCCCGGAGCTGATGAATTTTATCAATGAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCTAAGCTTACCAGTGTCTGTTGGGACAAGTGCATAACTGGAACACCAGGGATTGTTGCTGCATTCCCTAAATCAGATTATTTTACATTACATCTTGATGAGAAGAGTAATGAACTGATATGGGTTTGGAGTAATTGGAATGTGCTTTCGTTCGGGCAACCTGCTGCCTACTTCTTTGTCGTCCCAAGAGGAAGTCGCTCAACGTGGAGCACTGCCTCATATGTGTGGATGAAGATAGGCAGCAGGCGTTCCAATTTTCATGTAAGTGGCTACATGGAATAG

Coding sequence (CDS)

ATGGATTCCTCGTCTCTTAACTCCCCGGAGCTGATGAATTTTATCAATGAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCTAAGCTTACCAGTGTCTGTTGGGACAAGTGCATAACTGGAACACCAGGGATTGTTGCTGCATTCCCTAAATCAGATTATTTTACATTACATCTTGATGAGAAGAGTAATGAACTGATATGGGTTTGGAGTAATTGGAATGTGCTTTCGTTCGGGCAACCTGCTGCCTACTTCTTTGTCGTCCCAAGAGGAAGTCGCTCAACGTGGAGCACTGCCTCATATGTGTGGATGAAGATAGGCAGCAGGCGTTCCAATTTTCATGTAAGTGGCTACATGGAATAG
Homology
[Close]
BLAST of CSPI01G00280 vs. Swiss-Prot
Match: TIM8_ARATH (Mitochondrial import inner membrane translocase subunit TIM8 OS=Arabidopsis thaliana GN=TIM8 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.3e-11
Identity = 30/45 (66.67%), Postives = 37/45 (82.22%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD S  N+PEL+ F+ +EK+RAMV EMV+K+TSVCWDKCIT  PG
Sbjct: 1  MDPSMANNPELLQFLAQEKERAMVNEMVSKMTSVCWDKCITSAPG 45

[Close]
BLAST of CSPI01G00280 vs. TrEMBL
Match: A0A0A0LTY7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G001280 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.4e-16
Identity = 45/45 (100.00%), Postives = 45/45 (100.00%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG
Sbjct: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 45

[Close]
BLAST of CSPI01G00280 vs. TrEMBL
Match: Q9XGY3_MALDO (Small zinc finger-like protein (Fragment) OS=Malus domestica GN=TIM8 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.6e-12
Identity = 35/45 (77.78%), Postives = 42/45 (93.33%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD S++N+PEL+NFIN+EK+RAMV EMV KLT+VCWDKCITGTPG
Sbjct: 1  MDPSAMNNPELLNFINQEKERAMVNEMVGKLTNVCWDKCITGTPG 45

[Close]
BLAST of CSPI01G00280 vs. TrEMBL
Match: M5WBB3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa014244mg PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 3.5e-12
Identity = 35/45 (77.78%), Postives = 41/45 (91.11%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD SS+N+PEL+ FIN+EK+RAMV EMV KLT+VCWDKCITGTPG
Sbjct: 1  MDPSSMNNPELLQFINQEKERAMVNEMVGKLTNVCWDKCITGTPG 45

[Close]
BLAST of CSPI01G00280 vs. TrEMBL
Match: W9RHB0_9ROSA (Mitochondrial import inner membrane translocase subunit Tim8 OS=Morus notabilis GN=L484_008830 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 7.8e-12
Identity = 34/45 (75.56%), Postives = 41/45 (91.11%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD S++NSPEL+ FIN+EK+RAM+ EMVAKLT+VCWDKCIT TPG
Sbjct: 1  MDPSAINSPELLQFINQEKERAMINEMVAKLTTVCWDKCITSTPG 45

[Close]
BLAST of CSPI01G00280 vs. TrEMBL
Match: A0A022RSI6_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a017418mg PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.0e-11
Identity = 34/45 (75.56%), Postives = 41/45 (91.11%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD+SSL+SPEL +FIN+EKQ+AMV EM+ KLT+ CWDKCITGTPG
Sbjct: 1  MDASSLSSPELQDFINQEKQKAMVNEMIGKLTAACWDKCITGTPG 45

[Close]
BLAST of CSPI01G00280 vs. TAIR10
Match: AT5G50810.1 (AT5G50810.1 translocase inner membrane subunit 8)

HSP 1 Score: 68.9 bits (167), Expect = 2.4e-12
Identity = 30/45 (66.67%), Postives = 37/45 (82.22%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD S  N+PEL+ F+ +EK+RAMV EMV+K+TSVCWDKCIT  PG
Sbjct: 1  MDPSMANNPELLQFLAQEKERAMVNEMVSKMTSVCWDKCITSAPG 45

[Close]
BLAST of CSPI01G00280 vs. NCBI nr
Match: gi|659128830|ref|XP_008464391.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Cucumis melo])

HSP 1 Score: 94.0 bits (232), Expect = 2.0e-16
Identity = 45/45 (100.00%), Postives = 45/45 (100.00%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG
Sbjct: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 45

[Close]
BLAST of CSPI01G00280 vs. NCBI nr
Match: gi|5107157|gb|AAD39991.1|AF150084_1 (small zinc finger-like protein [Malus domestica])

HSP 1 Score: 80.5 bits (197), Expect = 2.3e-12
Identity = 35/45 (77.78%), Postives = 42/45 (93.33%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD S++N+PEL+NFIN+EK+RAMV EMV KLT+VCWDKCITGTPG
Sbjct: 1  MDPSAMNNPELLNFINQEKERAMVNEMVGKLTNVCWDKCITGTPG 45

[Close]
BLAST of CSPI01G00280 vs. NCBI nr
Match: gi|694429363|ref|XP_009342209.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM8-like [Pyrus x bretschneideri])

HSP 1 Score: 80.5 bits (197), Expect = 2.3e-12
Identity = 35/45 (77.78%), Postives = 42/45 (93.33%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD S++N+PEL+NFIN+EK+RAMV EMV KLT+VCWDKCITGTPG
Sbjct: 1  MDPSAMNNPELLNFINQEKERAMVNEMVGKLTNVCWDKCITGTPG 45

[Close]
BLAST of CSPI01G00280 vs. NCBI nr
Match: gi|747053860|ref|XP_011073110.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Sesamum indicum])

HSP 1 Score: 79.7 bits (195), Expect = 3.9e-12
Identity = 36/57 (63.16%), Postives = 44/57 (77.19%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPGIVAAFPKSDYFT 58
          MD SSLNSPEL  F+N+EK++AMV EM+ KLTS CWDKCITGTPG   +  +S+  T
Sbjct: 1  MDPSSLNSPELQQFLNQEKEKAMVTEMIGKLTSACWDKCITGTPGSKFSSSESNCLT 57

[Close]
BLAST of CSPI01G00280 vs. NCBI nr
Match: gi|595849876|ref|XP_007209810.1| (hypothetical protein PRUPE_ppa014244mg [Prunus persica])

HSP 1 Score: 79.3 bits (194), Expect = 5.0e-12
Identity = 35/45 (77.78%), Postives = 41/45 (91.11%), Query Frame = 1 

Query: 1  MDSSSLNSPELMNFINEEKQRAMVAEMVAKLTSVCWDKCITGTPG 46
          MD SS+N+PEL+ FIN+EK+RAMV EMV KLT+VCWDKCITGTPG
Sbjct: 1  MDPSSMNNPELLQFINQEKERAMVNEMVGKLTNVCWDKCITGTPG 45

The following BLAST results are available for this feature:
BLAST of CSPI01G00280 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TIM8_ARATH4.3e-1166.67Mitochondrial import inner membrane translocase subunit TIM8 OS=Arabidopsis thal... [more]
BLAST of CSPI01G00280 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LTY7_CUCSA1.4e-16100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G001280 PE=3 SV=1[more]
Q9XGY3_MALDO1.6e-1277.78Small zinc finger-like protein (Fragment) OS=Malus domestica GN=TIM8 PE=2 SV=1[more]
M5WBB3_PRUPE3.5e-1277.78Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa014244mg PE=3 SV=1[more]
W9RHB0_9ROSA7.8e-1275.56Mitochondrial import inner membrane translocase subunit Tim8 OS=Morus notabilis ... [more]
A0A022RSI6_ERYGU1.0e-1175.56Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a017418mg PE=3 SV=1[more]
BLAST of CSPI01G00280 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G50810.12.4e-1266.67 translocase inner membrane subunit 8[more]
BLAST of CSPI01G00280 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659128830|ref|XP_008464391.1|2.0e-16100.00PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Cucumis... [more]
gi|5107157|gb|AAD39991.1|AF150084_12.3e-1277.78small zinc finger-like protein [Malus domestica][more]
gi|694429363|ref|XP_009342209.1|2.3e-1277.78PREDICTED: mitochondrial import inner membrane translocase subunit TIM8-like [Py... [more]
gi|747053860|ref|XP_011073110.1|3.9e-1263.16PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Sesamum... [more]
gi|595849876|ref|XP_007209810.1|5.0e-1277.78hypothetical protein PRUPE_ppa014244mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004217Tim10-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
biological_process GO:0008643 carbohydrate transport
biological_process GO:0008150 biological_process
biological_process GO:0072321 chaperone-mediated protein transport
cellular_component GO:0005758 mitochondrial intermembrane space
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G00280.1CSPI01G00280.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004217Tim10/DDP family zinc fingerGENE3DG3DSA:1.10.287.810coord: 16..44
score: 6.
IPR004217Tim10/DDP family zinc fingerPFAMPF02953zf-Tim10_DDPcoord: 13..44
score: 2.
IPR004217Tim10/DDP family zinc fingerunknownSSF144122Tim10-likecoord: 20..43
score: 7.8

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI01G00280Cla019302Watermelon (97103) v1cpiwmB099
CSPI01G00280Csa1G001280Cucumber (Chinese Long) v2cpicuB000
CSPI01G00280MELO3C026182Melon (DHL92) v3.5.1cpimeB055
CSPI01G00280ClCG06G018150Watermelon (Charleston Gray)cpiwcgB083
CSPI01G00280Lsi06G016640Bottle gourd (USVL1VR-Ls)cpilsiB070
CSPI01G00280MELO3C026182.2Melon (DHL92) v3.6.1cpimedB049
CSPI01G00280CsaV3_1G000290Cucumber (Chinese Long) v3cpicucB001
CSPI01G00280Cla97C06G127920Watermelon (97103) v2cpiwmbB079
CSPI01G00280Cucsa.157100Cucumber (Gy14) v1cgycpiB229
CSPI01G00280CmaCh11G000150Cucurbita maxima (Rimu)cmacpiB085
CSPI01G00280CmaCh10G000380Cucurbita maxima (Rimu)cmacpiB049
CSPI01G00280CmoCh11G000140Cucurbita moschata (Rifu)cmocpiB079
CSPI01G00280Cp4.1LG18g09230Cucurbita pepo (Zucchini)cpecpiB361
CSPI01G00280Cp4.1LG04g16430Cucurbita pepo (Zucchini)cpecpiB656
CSPI01G00280CsGy1G000290Cucumber (Gy14) v2cgybcpiB001
CSPI01G00280Carg10260Silver-seed gourdcarcpiB0396
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI01G00280Melon (DHL92) v3.6.1cpimedB014
CSPI01G00280Cucumber (Gy14) v2cgybcpiB013
CSPI01G00280Cucumber (Gy14) v2cgybcpiB095
CSPI01G00280Silver-seed gourdcarcpiB0642
CSPI01G00280Cucumber (Chinese Long) v3cpicucB015
CSPI01G00280Cucumber (Chinese Long) v3cpicucB028
CSPI01G00280Cucumber (Chinese Long) v3cpicucB053
CSPI01G00280Watermelon (97103) v2cpiwmbB051
CSPI01G00280Wax gourdcpiwgoB061
CSPI01G00280Wax gourdcpiwgoB081
CSPI01G00280Wild cucumber (PI 183967)cpicpiB003
CSPI01G00280Wild cucumber (PI 183967)cpicpiB014
CSPI01G00280Cucurbita maxima (Rimu)cmacpiB227
CSPI01G00280Cucurbita moschata (Rifu)cmocpiB040
CSPI01G00280Cucurbita moschata (Rifu)cmocpiB213
CSPI01G00280Cucurbita moschata (Rifu)cmocpiB396
CSPI01G00280Cucumber (Chinese Long) v2cpicuB009
CSPI01G00280Cucumber (Chinese Long) v2cpicuB024
CSPI01G00280Melon (DHL92) v3.5.1cpimeB015
CSPI01G00280Melon (DHL92) v3.5.1cpimeB079
CSPI01G00280Watermelon (Charleston Gray)cpiwcgB053
CSPI01G00280Watermelon (97103) v1cpiwmB029
CSPI01G00280Cucurbita pepo (Zucchini)cpecpiB515
CSPI01G00280Bottle gourd (USVL1VR-Ls)cpilsiB001
CSPI01G00280Bottle gourd (USVL1VR-Ls)cpilsiB041