Bhi05G000749 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi05G000749
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cysteine proteinase inhibitor
Location	chr5 : 31890076 .. 31890647 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGATAAACGCAAAGCCATCACAAAATTTCTTCCATATACAAAAGTTTTACAATGAGTTGTGGAGCGATTGCCGATGCCAAGCATGAGATAAAGGATGCAAAGAAAGGTTATGTGTGGATCACATGGACACCAATTCCCAACATTGGAGATCTGTTCGTGAAAGAAATTGCAAAGTTCGCAGTGGAAGAATACAATAAGAAATACAAAGCACATTGGAAATGCAAACGCATTCAAAAAGGTTGGTACACAGTGATGGATAATAATCAAATTCAATATCGTTTTCATTTAGAGCAAGATGAAGTTCCTCAAGGAGATGAGAAGATATATGAGGCTGTTGTGTCAGGAGAGCAATCCCATGAGTTGCCTTTTCTACTAATAATTAACAAGAATCTCCTACTTTTCATGCCATACTTCAATTATTGACCTAACTTTCACTTGTATTCTACAATAATAATACTCATTACATCACTTATGAAGTAGTGAGTTTGATTATCTATATCTAAACTATAGATTATAAATAAAAGCTACTTATGTAATGATCTTTTCTCTTCATTTTCATGTTAATGGCG

mRNA sequence

GAAAGATAAACGCAAAGCCATCACAAAATTTCTTCCATATACAAAAGTTTTACAATGAGTTGTGGAGCGATTGCCGATGCCAAGCATGAGATAAAGGATGCAAAGAAAGGTTATGTGTGGATCACATGGACACCAATTCCCAACATTGGAGATCTGTTCGTGAAAGAAATTGCAAAGTTCGCAGTGGAAGAATACAATAAGAAATACAAAGCACATTGGAAATGCAAACGCATTCAAAAAGGTTGGTACACAGTGATGGATAATAATCAAATTCAATATCGTTTTCATTTAGAGCAAGATGAAGTTCCTCAAGGAGATGAGAAGATATATGAGGCTGTTGTGTCAGGAGAGCAATCCCATGAGTTGCCTTTTCTACTAATAATTAACAAGAATCTCCTACTTTTCATGCCATACTTCAATTATTGACCTAACTTTCACTTGTATTCTACAATAATAATACTCATTACATCACTTATGAAGTAGTGAGTTTGATTATCTATATCTAAACTATAGATTATAAATAAAAGCTACTTATGTAATGATCTTTTCTCTTCATTTTCATGTTAATGGCG

Coding sequence (CDS)

ATGAGTTGTGGAGCGATTGCCGATGCCAAGCATGAGATAAAGGATGCAAAGAAAGGTTATGTGTGGATCACATGGACACCAATTCCCAACATTGGAGATCTGTTCGTGAAAGAAATTGCAAAGTTCGCAGTGGAAGAATACAATAAGAAATACAAAGCACATTGGAAATGCAAACGCATTCAAAAAGGTTGGTACACAGTGATGGATAATAATCAAATTCAATATCGTTTTCATTTAGAGCAAGATGAAGTTCCTCAAGGAGATGAGAAGATATATGAGGCTGTTGTGTCAGGAGAGCAATCCCATGAGTTGCCTTTTCTACTAATAATTAACAAGAATCTCCTACTTTTCATGCCATACTTCAATTATTGA

Protein sequence

MSCGAIADAKHEIKDAKKGYVWITWTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQYRFHLEQDEVPQGDEKIYEAVVSGEQSHELPFLLIINKNLLLFMPYFNY

Homology

[Close]

BLAST of Bhi05G000749 vs. TrEMBL
Match: tr|A0A2P2NS36|A0A2P2NS36_RHIMU (Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.0e-04
Identity = 32/72 (44.44%), Postives = 43/72 (59.72%), Query Frame = 0


Query: 25 WTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQYRFHLEQDEV 84
          WTPI N+ D  V+EIA +AV EYN +  A+ K +R+ KG   V+    I+YR  L   E 
Sbjct: 32 WTPITNLSDPHVQEIASYAVREYNHRSNANLKLQRLVKGEKQVVSG--IKYRLVLAVKE- 91

Query: 85 PQGDEKIYEAVV 97
            G +K Y+AVV
Sbjct: 92 -GGSDKKYQAVV 99

[Close]

BLAST of Bhi05G000749 vs. TrEMBL
Match: tr|A0A2P5X992|A0A2P5X992_GOSBA (Cysteine proteinase inhibitor OS=Gossypium barbadense OX=3634 GN=GOBAR_AA20764 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 6.5e-04
Identity = 35/83 (42.17%), Postives = 48/83 (57.83%), Query Frame = 0


Query: 15  DAKKGYVWITWTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQ 74
           DA+K  +   WTPI NI D  V EIA+FA++EYNK+ K + K + + KG   V+    I 
Sbjct: 23  DARKDALTGGWTPIKNIKDPHVTEIAEFAIDEYNKQSKGNLKLEEVVKGKTQVVSG--IN 82

Query: 75  YRFHLE-QDEVPQGDEKIYEAVV 97
           Y   L+ +DE     +  YEAVV
Sbjct: 83  YTLVLQVKDETV---DNSYEAVV 100

[Close]

BLAST of Bhi05G000749 vs. TrEMBL
Match: tr|P94012|P94012_CUCMA (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=PP1 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 8.5e-04
Identity = 26/76 (34.21%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 25  WTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQYRFHLEQDEV 84
           W  IP++    V+E+AK AVE++N ++    K   I +GWY  +  N ++YR H+   + 
Sbjct: 17  WIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHIRAIDF 76

Query: 85  PQGDEKIYEAVVSGEQ 101
            Q    +YEA+V  E+
Sbjct: 77  LQ-RSLLYEALVFEEK 91

[Close]

BLAST of Bhi05G000749 vs. NCBI nr
Match: XP_023522970.1 (uncharacterized protein LOC111787034 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 55.1 bits (131), Expect = 2.0e-04
Identity = 28/93 (30.11%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 25  WTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQYRFHLEQDE- 84
           W  IP++ ++ V+ IAKFAV++YN  +K  +K K I+ GW+  +    + +  H+E  + 
Sbjct: 28  WIQIPDVSEVCVQNIAKFAVQDYNLIHKESFKYKSIKCGWFMELKGEDLAFHLHIEVKDC 87

Query: 85  ---VPQGDEKIYEAVVSGEQSHELPFLLIINKN 114
              + + D  + E    GE+  +L    +I KN
Sbjct: 88  LGRIFELDVVVSEKKACGEKVRKLESAKLIKKN 120

[Close]

BLAST of Bhi05G000749 vs. NCBI nr
Match: XP_007040765.2 (PREDICTED: cysteine proteinase inhibitor 5 [Theobroma cacao])

HSP 1 Score: 54.7 bits (130), Expect = 2.6e-04
Identity = 31/82 (37.80%), Postives = 46/82 (56.10%), Query Frame = 0


Query: 15  DAKKGYVWITWTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQ 74
           DA++  +   W PI ++ D  VKE+A+FAV EYNK+ K+  + K + KG   V+    I 
Sbjct: 23  DARRENLAGGWEPIKDLNDPHVKEVAEFAVSEYNKQSKSSLELKSVMKGEMQVVSG--IN 82

Query: 75  YRFHLEQDEVPQGDEKIYEAVV 97
           Y+  +E       + K YEAVV
Sbjct: 83  YKLDVETTAGTAAEAKEYEAVV 102

[Close]

BLAST of Bhi05G000749 vs. NCBI nr
Match: XP_021289294.1 (cysteine proteinase inhibitor 5-like [Herrania umbratica])

HSP 1 Score: 53.9 bits (128), Expect = 4.4e-04
Identity = 31/82 (37.80%), Postives = 45/82 (54.88%), Query Frame = 0


Query: 15  DAKKGYVWITWTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQ 74
           DA++  +   W PI ++ D  V E+AKFAV EYNK+ K+  + K + KG   V+    I 
Sbjct: 23  DARRKTLVGGWKPIKDLNDPHVTEVAKFAVSEYNKQSKSSLELKSVMKGEMQVVSG--IN 82

Query: 75  YRFHLEQDEVPQGDEKIYEAVV 97
           Y+  +E       + K YEAVV
Sbjct: 83  YKLDVETTAGTTAEAKEYEAVV 102

[Close]

BLAST of Bhi05G000749 vs. NCBI nr
Match: XP_022927699.1 (uncharacterized protein LOC111434517 [Cucurbita moschata])

HSP 1 Score: 53.1 bits (126), Expect = 7.6e-04
Identity = 29/72 (40.28%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 25  WTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQYRFHLEQDEV 84
           W  IPN+  +FV EI+KFAV E+N KYK +     I +GWY  M  + +++R  L+  + 
Sbjct: 124 WIKIPNLQAVFVIEISKFAVHEFNIKYKDYLVFDSIYEGWYMEMGRDDLKFRLKLKAIDC 183

Query: 85  PQGDEKIYEAVV 97
            +     YEAVV
Sbjct: 184 LKRVNS-YEAVV 194

[Close]

BLAST of Bhi05G000749 vs. NCBI nr
Match: XP_022927698.1 (uncharacterized protein LOC111434515 [Cucurbita moschata])

HSP 1 Score: 53.1 bits (126), Expect = 7.6e-04
Identity = 28/72 (38.89%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 25  WTPIPNIGDLFVKEIAKFAVEEYNKKYKAHWKCKRIQKGWYTVMDNNQIQYRFHLEQDEV 84
           W  IPN+    V+E+AKFAV+E+N K     K   +  GW+  +  + I++RFHL+  + 
Sbjct: 517 WIKIPNVQVPLVQELAKFAVDEHNNKSGEGLKYVEVYDGWFMDLGQDNIKFRFHLKAKDW 576

Query: 85  PQGDEKIYEAVV 97
             G  + YEAVV
Sbjct: 577 -LGRVRSYEAVV 587

The following BLAST results are available for this feature:

BLAST of Bhi05G000749 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Bhi05G000749 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Bhi05G000749 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A2P2NS36\|A0A2P2NS36_RHIMU	5.0e-04	44.44	Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=3 SV=1	[more]
tr\|A0A2P5X992\|A0A2P5X992_GOSBA	6.5e-04	42.17	Cysteine proteinase inhibitor OS=Gossypium barbadense OX=3634 GN=GOBAR_AA20764 P...	[more]
tr\|P94012\|P94012_CUCMA	8.5e-04	34.21	Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=PP1 PE=3 SV=1	[more]

BLAST of Bhi05G000749 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023522970.1	2.0e-04	30.11	uncharacterized protein LOC111787034 [Cucurbita pepo subsp. pepo]	[more]
XP_007040765.2	2.6e-04	37.80	PREDICTED: cysteine proteinase inhibitor 5 [Theobroma cacao]	[more]
XP_021289294.1	4.4e-04	37.80	cysteine proteinase inhibitor 5-like [Herrania umbratica]	[more]
XP_022927699.1	7.6e-04	40.28	uncharacterized protein LOC111434517 [Cucurbita moschata]	[more]
XP_022927698.1	7.6e-04	38.89	uncharacterized protein LOC111434515 [Cucurbita moschata]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027214	Cystatin
IPR009994	PP1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0030414	peptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000749	Bhi05M000749	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 21..116 e-value: 8.4E-13 score: 50.2
None	No IPR available	PANTHER	PTHR11413:SF65	CYSTEINE PROTEINASE INHIBITOR 5	coord: 23..102
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 25..100
IPR009994	Phloem filament PP1	PFAM	PF07430	PP1	coord: 35..101 e-value: 1.4E-10 score: 41.0
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 23..102

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi05G000749	MELO3C018594	Melon (DHL92) v3.5.1	mewgoB219
Bhi05G000749	ClCG09G015870	Watermelon (Charleston Gray)	wcgwgoB033
Bhi05G000749	CSPI07G06030	Wild cucumber (PI 183967)	cpiwgoB686
Bhi05G000749	CmaCh19G004720	Cucurbita maxima (Rimu)	cmawgoB0642
Bhi05G000749	CmoCh19G004880	Cucurbita moschata (Rifu)	cmowgoB0642
Bhi05G000749	Lsi03G007230	Bottle gourd (USVL1VR-Ls)	lsiwgoB320
Bhi05G000749	CsGy7G005690	Cucumber (Gy14) v2	cgybwgoB646
Bhi05G000749	MELO3C018594.2	Melon (DHL92) v3.6.1	medwgoB219
Bhi05G000749	CsaV3_7G006990	Cucumber (Chinese Long) v3	cucwgoB700
Bhi05G000749	Cla012896	Watermelon (97103) v1	wgowmB243
Bhi05G000749	Cla97C09G177710	Watermelon (97103) v2	wgowmbB263

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi05G000749	Cucumber (Gy14) v1	cgywgoB445
Bhi05G000749	Cucumber (Gy14) v1	cgywgoB451
Bhi05G000749	Cucumber (Gy14) v2	cgybwgoB632
Bhi05G000749	Cucurbita maxima (Rimu)	cmawgoB0116
Bhi05G000749	Cucurbita pepo (Zucchini)	cpewgoB0317
Bhi05G000749	Wild cucumber (PI 183967)	cpiwgoB672
Bhi05G000749	Cucumber (Chinese Long) v3	cucwgoB686
Bhi05G000749	Cucumber (Chinese Long) v2	cuwgoB661
Bhi05G000749	Cucumber (Chinese Long) v2	cuwgoB675
Bhi05G000749	Melon (DHL92) v3.6.1	medwgoB220
Bhi05G000749	Melon (DHL92) v3.6.1	medwgoB320
Bhi05G000749	Melon (DHL92) v3.5.1	mewgoB220
Bhi05G000749	Melon (DHL92) v3.5.1	mewgoB321
Bhi05G000749	Watermelon (Charleston Gray)	wcgwgoB050
Bhi05G000749	Watermelon (97103) v2	wgowmbB279
Bhi05G000749	Watermelon (97103) v1	wgowmB261
Bhi05G000749	Silver-seed gourd	carwgoB0377