Bhi03G000481 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi03G000481
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	RING/U-box superfamily protein
Location	chr3 : 12114564 .. 12116089 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCAATGTGGTACCGCATTTTCCAAAGAAATAAGAGAGAGACTCTGTAAAGTGTAGACTCCCTCCACTTCCTCTGAGTTCCTCATTCTAATTTCTTTGTCTTCTTCTTCTTCATTTCTCTCTTAATTGCTTAATTAATTTTGGCGTCCATTAAACTGGCATTTAAGACTCTCACGATTCTCGTATTTTTGTTCTTGGGGCCTGGTTTTAGTTTCGAATTTCGACCCCTTACGGGGTTCCGAGAATAATTAAGAACAGAGAGGTGGGTTTCATCAGAATCTTTGAATAAACTCACGAGATCTCCATGGCCATGGCTAAAGGTTATTAATCCTAATCCTAACTATTTACATTTCCTCTCAATTTTCTTCATTCATTTTCATACTTTTTTTCGATTTTCTTCACTAAATTTTGTTTCCCCATCGGAAAAATGAGTCTTCTCAGTGTGATTTTACAGGAAGCTGCTCGGCGAGAAAGGGAGGCTAGGACCATTTGGGGATTTTTAACCGATCAAATGGACGGTGTCGATGGAGGAGGAAGGCAGCGGCCCAGTTTAAAGCAACGGCTAGGATTTAAGGTTATGGGCTGTTGTGGTGCTACTTGGGGTTTCCGGTCGCCGACTGTAAGCGTCAGAAACGGCGGCGGCGATGACGATGACCGGAGAGTACCGGATGTGGAGGTGACGAACACGCGCCGGGAGGAAAGGGAGTTGGATCGGAGTTGCTTGAGTCCGTTGTCCGTGCAGTCGCCGGGGCCGTCCGGGATGAACTTGGCCACAGCTCTAGCGGCGGAGCGTCGTTTAAGAGCGTCCCCACGTGGAATGGAAGGGGGCATTGTGGAATTTAATAACAACGATTTTGACTCACTGGGCGGAATGATGGGAACGGGGACGCCGTTGAGGGTGTCGCTGATGCGGCTGCTGGAAGAAACAGACGGCGGTCGTGGCGGTTGTGGGAATTTGGGAGTGGCGGAAACAAAGAGAGAAGAGCCGGGAAATGATTCGATGTGTTGCGTGTGCATGGGAAGGAAAAAAGGAGCGGCATTTATCCCATGTGGGCATACGTTTTGTAGGGTTTGTTCGAGGGAGCTGTGGCTAAATCGAGGATCTTGTCCGCTTTGCAACCGTCCGATTATAGAGATTCTTGATATATTTTGAAGGACCATGATTAATACGATGCGGATGAGAACAGAGGATTGTTGGTGACGGGAAGGGCCTAGTGCTTTTGCCTCAAGATTCGCGACAGACTGTTTTCATTGGACGGCACTGAACGATGGCCGTATCTGGGCGCCATGTGTATGCGATGCGGATGACAAAACTTTTTCACGTCCGAGAAAAAAAAAAAAAGTTTAATTAATAATTTAATAGTTTCGATGTAATTCATATAATTTTAAACTTTATACATGGGA

mRNA sequence

CTTCAATGTGGTACCGCATTTTCCAAAGAAATAAGAGAGAGACTCTGTAAAGTGTAGACTCCCTCCACTTCCTCTGAGTTCCTCATTCTAATTTCTTTGTCTTCTTCTTCTTCATTTCTCTCTTAATTGCTTAATTAATTTTGGCGTCCATTAAACTGGCATTTAAGACTCTCACGATTCTCGTATTTTTGTTCTTGGGGCCTGGTTTTAGTTTCGAATTTCGACCCCTTACGGGGTTCCGAGAATAATTAAGAACAGAGAGGTGGGTTTCATCAGAATCTTTGAATAAACTCACGAGATCTCCATGGCCATGGCTAAAGTGTGATTTTACAGGAAGCTGCTCGGCGAGAAAGGGAGGCTAGGACCATTTGGGGATTTTTAACCGATCAAATGGACGGTGTCGATGGAGGAGGAAGGCAGCGGCCCAGTTTAAAGCAACGGCTAGGATTTAAGGTTATGGGCTGTTGTGGTGCTACTTGGGGTTTCCGGTCGCCGACTGTAAGCGTCAGAAACGGCGGCGGCGATGACGATGACCGGAGAGTACCGGATGTGGAGGTGACGAACACGCGCCGGGAGGAAAGGGAGTTGGATCGGAGTTGCTTGAGTCCGTTGTCCGTGCAGTCGCCGGGGCCGTCCGGGATGAACTTGGCCACAGCTCTAGCGGCGGAGCGTCGTTTAAGAGCGTCCCCACGTGGAATGGAAGGGGGCATTGTGGAATTTAATAACAACGATTTTGACTCACTGGGCGGAATGATGGGAACGGGGACGCCGTTGAGGGTGTCGCTGATGCGGCTGCTGGAAGAAACAGACGGCGGTCGTGGCGGTTGTGGGAATTTGGGAGTGGCGGAAACAAAGAGAGAAGAGCCGGGAAATGATTCGATGTGTTGCGTGTGCATGGGAAGGAAAAAAGGAGCGGCATTTATCCCATGTGGGCATACGTTTTGTAGGGTTTGTTCGAGGGAGCTGTGGCTAAATCGAGGATCTTGTCCGCTTTGCAACCGTCCGATTATAGAGATTCTTGATATATTTTGAAGGACCATGATTAATACGATGCGGATGAGAACAGAGGATTGTTGGTGACGGGAAGGGCCTAGTGCTTTTGCCTCAAGATTCGCGACAGACTGTTTTCATTGGACGGCACTGAACGATGGCCGTATCTGGGCGCCATGTGTATGCGATGCGGATGACAAAACTTTTTCACGTCCGAGAAAAAAAAAAAAAGTTTAATTAATAATTTAATAGTTTCGATGTAATTCATATAATTTTAAACTTTATACATGGGA

Coding sequence (CDS)

ATGGACGGTGTCGATGGAGGAGGAAGGCAGCGGCCCAGTTTAAAGCAACGGCTAGGATTTAAGGTTATGGGCTGTTGTGGTGCTACTTGGGGTTTCCGGTCGCCGACTGTAAGCGTCAGAAACGGCGGCGGCGATGACGATGACCGGAGAGTACCGGATGTGGAGGTGACGAACACGCGCCGGGAGGAAAGGGAGTTGGATCGGAGTTGCTTGAGTCCGTTGTCCGTGCAGTCGCCGGGGCCGTCCGGGATGAACTTGGCCACAGCTCTAGCGGCGGAGCGTCGTTTAAGAGCGTCCCCACGTGGAATGGAAGGGGGCATTGTGGAATTTAATAACAACGATTTTGACTCACTGGGCGGAATGATGGGAACGGGGACGCCGTTGAGGGTGTCGCTGATGCGGCTGCTGGAAGAAACAGACGGCGGTCGTGGCGGTTGTGGGAATTTGGGAGTGGCGGAAACAAAGAGAGAAGAGCCGGGAAATGATTCGATGTGTTGCGTGTGCATGGGAAGGAAAAAAGGAGCGGCATTTATCCCATGTGGGCATACGTTTTGTAGGGTTTGTTCGAGGGAGCTGTGGCTAAATCGAGGATCTTGTCCGCTTTGCAACCGTCCGATTATAGAGATTCTTGATATATTTTGA

Protein sequence

MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGGGDDDDRRVPDVEVTNTRREERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF

Homology

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BLAST of Bhi03G000481 vs. Swiss-Prot
Match: sp|Q8LA32|LUL4_ARATH (Probable E3 ubiquitin-protein ligase LUL4 OS=Arabidopsis thaliana OX=3702 GN=LUL4 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 8.6e-07
Identity = 23/57 (40.35%), Postives = 34/57 (59.65%), Query Frame = 0


Query: 157 EEPGNDSMCCVCMGRKKGAAFIPCGH-TFCRVCSRELWLNRGSCPLCNRPIIEILDI 213
           +E G+ + C +CM   K  A +PC H   C  C++EL L    CP+C +PI E+L+I
Sbjct: 294 DESGSGTECVICMTEAKDTAVLPCRHLCMCSDCAKELRLQSNKCPICRQPIEELLEI 350

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BLAST of Bhi03G000481 vs. Swiss-Prot
Match: sp|Q8BFW4|TRI65_MOUSE (Tripartite motif-containing protein 65 OS=Mus musculus OX=10090 GN=Trim65 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.1e-04
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = 0


Query: 165 CCVCMGRKKGAAFIPCGHTFCRVCSRELWLN-RGSCPLCNRPIIE 209
           C +C+GR +    +PCGH+FC  C ++ W +   SCP C +P  E
Sbjct: 13  CSICLGRYRDPVTLPCGHSFCGNCIQDSWRSCEKSCPECRQPFPE 57

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BLAST of Bhi03G000481 vs. Swiss-Prot
Match: sp|Q8IUD6|RN135_HUMAN (E3 ubiquitin-protein ligase RNF135 OS=Homo sapiens OX=9606 GN=RNF135 PE=1 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 5.2e-04
Identity = 19/48 (39.58%), Postives = 25/48 (52.08%), Query Frame = 0


Query: 161 NDSMCCVCMGRKKGAAFIPCGHTFCRVCSRELW----LNRGSCPLCNR 205
           +D  C +C G     A +PCGH+FCR C   LW      R +CP C +
Sbjct: 17  DDLGCIICQGLLDWPATLPCGHSFCRHCLEALWGARDARRWACPTCRQ 64

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BLAST of Bhi03G000481 vs. TAIR10
Match: AT4G03965.1 (RING/U-box superfamily protein)

HSP 1 Score: 179.5 bits (454), Expect = 2.2e-45
Identity = 107/226 (47.35%), Postives = 124/226 (54.87%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTR 60
           MDG+D   R++ +LK+RLGFK +GCCG TWG R                       +NT 
Sbjct: 29  MDGLDRSRRRKRTLKERLGFKRIGCCGPTWGLRL---------------------TSNTG 88

Query: 61  REERELDRSCLSPLSVQSPGP--SGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSL 120
            ++ E   + L   S    GP   GMNLATALAAER  R       G +           
Sbjct: 89  EDDDEPFENRLVSGSDHVTGPLNQGMNLATALAAERNYRTEETEASGSL----------- 148

Query: 121 GGMMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREE-----------PGNDSMCCV 180
                  TPL+VSLMRLLEET         + V E + E              NDS+CCV
Sbjct: 149 -------TPLKVSLMRLLEET------AERVVVEENETERVTASASSTVRGVNNDSVCCV 208

Query: 181 CMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           CMGRKKGAAFIPCGHTFCRVCSRE+WLNRGSCPLCNRPIIEILDI+
Sbjct: 209 CMGRKKGAAFIPCGHTFCRVCSREVWLNRGSCPLCNRPIIEILDIY 209

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BLAST of Bhi03G000481 vs. TAIR10
Match: AT4G22250.1 (RING/U-box superfamily protein)

HSP 1 Score: 164.1 bits (414), Expect = 9.6e-41
Identity = 100/212 (47.17%), Postives = 112/212 (52.83%), Query Frame = 0


Query: 9   RQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTRREERELDR 68
           R++ +LK+RL FK +GCCG TW  R    +  +               T+   E  EL  
Sbjct: 38  RRKRTLKERLRFKCIGCCGPTWNLRLTNNTTLHSP-------------TSRDDEIEELQI 97

Query: 69  SCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSLGGMMGTGTPL 128
                L       +GMNLATAL AER  R      E  +                  TP 
Sbjct: 98  ETGLVLESDHGSGTGMNLATALEAERYNRGESTEAEADM------------------TPR 157

Query: 129 RVSLMRLLEET-------DGGRGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAFIPCG 188
           RVSLMRLLEET       DG         +        GNDS+CCVCMGRKKGAAFIPCG
Sbjct: 158 RVSLMRLLEETAEKIVDDDGKETEILTASIGTL----TGNDSVCCVCMGRKKGAAFIPCG 214

Query: 189 HTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           HTFCRVCSRELWLNRGSCPLCNRPIIEILDIF
Sbjct: 218 HTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214

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BLAST of Bhi03G000481 vs. TAIR10
Match: AT1G62370.1 (RING/U-box superfamily protein)

HSP 1 Score: 124.4 bits (311), Expect = 8.4e-29
Identity = 88/217 (40.55%), Postives = 113/217 (52.07%), Query Frame = 0


Query: 2   DGVDGGGR---QRPSLKQRLGFKVMGCCG--ATWGFRSPTVSVRNGXXXXXXRRVPDVEV 61
           D ++GG R   +  +LKQRL F  +GCCG   T   R     +   XXXXXX     V  
Sbjct: 29  DQINGGDRTMSKWTTLKQRLRFDWVGCCGKPLTLRLRQAETPIXXXXXXXXXXXXXXVVD 88

Query: 62  TNTRREERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFD 121
            +      ELD  CL+  S +       NLA ALA ER    +    E  +         
Sbjct: 89  FSDPGTGTELD--CLTRGSTR-------NLAEALAEERLAHVTEE--ETAVT-------- 148

Query: 122 SLGGMMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAA 181
                       +V LMRLL E+DG           ++     GND +CCVCMGR+KGAA
Sbjct: 149 ------------KVPLMRLLAESDG----------CDSTTTWLGNDPLCCVCMGREKGAA 204

Query: 182 FIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           FIPCGHT+CRVCSRE+W+NRG+CPLCNR I ++LD++
Sbjct: 209 FIPCGHTYCRVCSREIWMNRGTCPLCNRSIFDVLDLY 204

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BLAST of Bhi03G000481 vs. TAIR10
Match: AT4G13100.2 (RING/U-box superfamily protein)

HSP 1 Score: 112.5 bits (280), Expect = 3.3e-25
Identity = 58/103 (56.31%), Postives = 66/103 (64.08%), Query Frame = 0


Query: 127 PLRVSLMRLLEETDG--------------GRGGC--GNLGVAETKREEPGNDSMCCVCMG 186
           P R+SLM LLE+ DG              GR GC  G   VA T   E G    CCVCM 
Sbjct: 167 PPRMSLMELLEDNDGHMYELSARDEVEVEGRDGCDGGGEAVAVTGAAELG----CCVCMV 226

Query: 187 RKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           R KGAAFIPCGHTFCR+CSRELW+ RG+CPLCN  I+++LDIF
Sbjct: 227 RSKGAAFIPCGHTFCRLCSRELWVQRGNCPLCNTAILQVLDIF 265

[Close]

BLAST of Bhi03G000481 vs. TAIR10
Match: AT3G25030.1 (RING/U-box superfamily protein)

HSP 1 Score: 109.4 bits (272), Expect = 2.8e-24
Identity = 51/88 (57.95%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 127 PLRVSLMRLLEETDGGRGGCGNLGVAETK-REEPGNDSMCCVCMGRKKGAAFIPCGHTFC 186
           P R+SLM LLEE +G        G AE +       +  CCVCM R KGAAFIPCGHTFC
Sbjct: 163 PARMSLMELLEENEGQMSFVSVDGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCGHTFC 222

Query: 187 RVCSRELWLNRGSCPLCNRPIIEILDIF 214
           R+CSRELW+ RG+CPLCN  I+E+LD+F
Sbjct: 223 RLCSRELWVQRGNCPLCNTTILEVLDLF 250

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BLAST of Bhi03G000481 vs. TrEMBL
Match: tr|A0A1S3AZT0|A0A1S3AZT0_CUCME (uncharacterized protein LOC103484644 OS=Cucumis melo OX=3656 GN=LOC103484644 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.6e-106
Identity = 190/213 (89.20%), Postives = 196/213 (92.02%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTR 60
           MDGVDGGGR+RPSLKQRLGFKVMGCCGATWGFR P+VSVR+G      RRVPD+EV NTR
Sbjct: 1   MDGVDGGGRRRPSLKQRLGFKVMGCCGATWGFRPPSVSVRDGGGEDDDRRVPDLEVMNTR 60

Query: 61  REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSLGG 120
           REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRG EGGIVEFNNNDFDS  G
Sbjct: 61  REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGAEGGIVEFNNNDFDSAVG 120

Query: 121 MMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAFIPC 180
           MM TGTPLRVSL+RLLEET+GG  G GNLGVAE KREE GNDSMCCVCMGRKKGAAFIPC
Sbjct: 121 MMETGTPLRVSLLRLLEETEGGCDGGGNLGVAEKKREETGNDSMCCVCMGRKKGAAFIPC 180

Query: 181 GHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           GHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF
Sbjct: 181 GHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 213

[Close]

BLAST of Bhi03G000481 vs. TrEMBL
Match: tr|A0A0A0KMY7|A0A0A0KMY7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G512860 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 3.8e-100
Identity = 182/215 (84.65%), Postives = 191/215 (88.84%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTR 60
           MDG+DGGGR+RPSLKQRLGFKVMGCCGATWGFR P+ SVR+G      RRVPD+EV NTR
Sbjct: 29  MDGIDGGGRRRPSLKQRLGFKVMGCCGATWGFRPPSDSVRDGGGEDDDRRVPDLEVMNTR 88

Query: 61  REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSLGG 120
           REEREL+RSCLSPLSVQSPGPSGMNLATALAAERRLRASPRG EG IVEFNNNDFDS  G
Sbjct: 89  REERELNRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGAEGDIVEFNNNDFDSAVG 148

Query: 121 MMGTGTPLRVSLMRLLEETDGG--RGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAFI 180
           MM TGTPLRVSL+RLLEETDGG       NLGVAE KR+E  NDSMCCVCMGRKKGAAFI
Sbjct: 149 MMETGTPLRVSLLRLLEETDGGCDXXXXXNLGVAEKKRDETRNDSMCCVCMGRKKGAAFI 208

Query: 181 PCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           PCGHTFCR+CSRELWLNRGSCPLCNRPIIEILDIF
Sbjct: 209 PCGHTFCRICSRELWLNRGSCPLCNRPIIEILDIF 243

[Close]

BLAST of Bhi03G000481 vs. TrEMBL
Match: tr|A0A2G9GM08|A0A2G9GM08_9LAMI (Putative E3 ubiquitin ligase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_21136 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.1e-54
Identity = 129/224 (57.59%), Postives = 143/224 (63.84%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTR 60
           MDGVD   R RPSLK+RLG + +GCCGATWG    TVSVR+          P VEV N  
Sbjct: 1   MDGVDRTMR-RPSLKERLGLRGLGCCGATWGLGPTTVSVRDEEEEDAGPLSPQVEVVNVV 60

Query: 61  REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIV------EFNNND 120
           +   E       P  VQSP  +GMNLA ALAAER+LRA      GG +        N+ND
Sbjct: 61  QNPTE--NGPALPCVVQSPAATGMNLAAALAAERQLRAVLDSENGGAIISPSPLRPNSND 120

Query: 121 FDS---LGGMMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREEPGN--DSMCCVCM 180
             S   + G    GTP RVSLMRLLEETDG  G        E K+EE G   DS+CCVCM
Sbjct: 121 ERSPTPVTGRNAPGTPSRVSLMRLLEETDGYDG--------EMKKEEDGGGCDSVCCVCM 180

Query: 181 GRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           GRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNR I+EILDI+
Sbjct: 181 GRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRSILEILDIY 213

[Close]

BLAST of Bhi03G000481 vs. TrEMBL
Match: tr|A0A061GHE7|A0A061GHE7_THECC (RING/U-box superfamily protein, putative isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_030318 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.0e-54
Identity = 132/219 (60.27%), Postives = 151/219 (68.95%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNG------XXXXXXRRVPDV 60
           MDGVD   R+R S K+RLGFK++GCCGATWG  S T+SVR+       XXXXXX++   V
Sbjct: 1   MDGVD-RERRRRSFKERLGFKIVGCCGATWGLGSTTISVRDDDEVEVVXXXXXXQQREQV 60

Query: 61  EVTNTRREERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNND 120
           E T++       D  C++P    SP  SGMNLA ALAAER  RA+ +  EGG      +D
Sbjct: 61  EATHSGHNP---DPGCINP----SPVSSGMNLAAALAAERHFRAA-QEPEGGNAA-PTSD 120

Query: 121 FDSLGGMMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREEPGNDSMCCVCMGRKKG 180
             S  G+   GTPLRVSLMRLLEE DG  GG    G  E    E G+DSMCCVCMG+KKG
Sbjct: 121 GQSNAGLRSPGTPLRVSLMRLLEEADGFCGG----GEQEKGGVEMGSDSMCCVCMGKKKG 180

Query: 181 AAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           AAFIPCGHTFCRVCSRELWLNRGSCPLCNR I+EILDIF
Sbjct: 181 AAFIPCGHTFCRVCSRELWLNRGSCPLCNRSILEILDIF 205

[Close]

BLAST of Bhi03G000481 vs. TrEMBL
Match: tr|A0A2N9EQT2|A0A2N9EQT2_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5015 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.5e-51
Identity = 129/223 (57.85%), Postives = 140/223 (62.78%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTR 60
           MDGVDG  R+R SLK RL FK +GCCGATW F   TV   N           D EV +  
Sbjct: 1   MDGVDGTTRRRRSLKDRLRFKGIGCCGATWVFGGTTVGDNNDE--------EDEEVVDVG 60

Query: 61  R--EERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSL 120
           +   E   D  C+ P+S      SGMNLA ALAAER+ RAS    E   V  N+ +  SL
Sbjct: 61  QGPSENNPDPDCVGPVS----SGSGMNLAAALAAERQFRASSNEQERDAVGPNDLNGVSL 120

Query: 121 GGMMGT--GTPLRVSLMRLLEETDGG------RGGCGNLGVAETKREEPGNDSMCCVCMG 180
            G +GT  GTPLRVSLMRLLEETDGG          G L  A       GNDSMCCVCM 
Sbjct: 121 -GTVGTAPGTPLRVSLMRLLEETDGGDEETIENEKAGTLDGAVA----TGNDSMCCVCMV 180

Query: 181 RKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           RKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNR I+EILDIF
Sbjct: 181 RKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRSILEILDIF 206

[Close]

BLAST of Bhi03G000481 vs. NCBI nr
Match: XP_008440052.1 (PREDICTED: uncharacterized protein LOC103484644 [Cucumis melo] >XP_008440053.1 PREDICTED: uncharacterized protein LOC103484644 [Cucumis melo])

HSP 1 Score: 392.9 bits (1008), Expect = 7.0e-106
Identity = 190/213 (89.20%), Postives = 196/213 (92.02%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTR 60
           MDGVDGGGR+RPSLKQRLGFKVMGCCGATWGFR P+VSVR+G      RRVPD+EV NTR
Sbjct: 1   MDGVDGGGRRRPSLKQRLGFKVMGCCGATWGFRPPSVSVRDGGGEDDDRRVPDLEVMNTR 60

Query: 61  REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSLGG 120
           REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRG EGGIVEFNNNDFDS  G
Sbjct: 61  REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGAEGGIVEFNNNDFDSAVG 120

Query: 121 MMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAFIPC 180
           MM TGTPLRVSL+RLLEET+GG  G GNLGVAE KREE GNDSMCCVCMGRKKGAAFIPC
Sbjct: 121 MMETGTPLRVSLLRLLEETEGGCDGGGNLGVAEKKREETGNDSMCCVCMGRKKGAAFIPC 180

Query: 181 GHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           GHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF
Sbjct: 181 GHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 213

[Close]

BLAST of Bhi03G000481 vs. NCBI nr
Match: KGN49071.1 (hypothetical protein Csa_6G512860 [Cucumis sativus])

HSP 1 Score: 373.2 bits (957), Expect = 5.7e-100
Identity = 182/215 (84.65%), Postives = 191/215 (88.84%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTR 60
           MDG+DGGGR+RPSLKQRLGFKVMGCCGATWGFR P+ SVR+G      RRVPD+EV NTR
Sbjct: 29  MDGIDGGGRRRPSLKQRLGFKVMGCCGATWGFRPPSDSVRDGGGEDDDRRVPDLEVMNTR 88

Query: 61  REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSLGG 120
           REEREL+RSCLSPLSVQSPGPSGMNLATALAAERRLRASPRG EG IVEFNNNDFDS  G
Sbjct: 89  REERELNRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGAEGDIVEFNNNDFDSAVG 148

Query: 121 MMGTGTPLRVSLMRLLEETDGG--RGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAFI 180
           MM TGTPLRVSL+RLLEETDGG       NLGVAE KR+E  NDSMCCVCMGRKKGAAFI
Sbjct: 149 MMETGTPLRVSLLRLLEETDGGCDXXXXXNLGVAEKKRDETRNDSMCCVCMGRKKGAAFI 208

Query: 181 PCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           PCGHTFCR+CSRELWLNRGSCPLCNRPIIEILDIF
Sbjct: 209 PCGHTFCRICSRELWLNRGSCPLCNRPIIEILDIF 243

[Close]

BLAST of Bhi03G000481 vs. NCBI nr
Match: XP_004134982.2 (PREDICTED: uncharacterized protein LOC101211804 [Cucumis sativus])

HSP 1 Score: 373.2 bits (957), Expect = 5.7e-100
Identity = 182/215 (84.65%), Postives = 191/215 (88.84%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRNGXXXXXXRRVPDVEVTNTR 60
           MDG+DGGGR+RPSLKQRLGFKVMGCCGATWGFR P+ SVR+G      RRVPD+EV NTR
Sbjct: 1   MDGIDGGGRRRPSLKQRLGFKVMGCCGATWGFRPPSDSVRDGGGEDDDRRVPDLEVMNTR 60

Query: 61  REERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDSLGG 120
           REEREL+RSCLSPLSVQSPGPSGMNLATALAAERRLRASPRG EG IVEFNNNDFDS  G
Sbjct: 61  REERELNRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGAEGDIVEFNNNDFDSAVG 120

Query: 121 MMGTGTPLRVSLMRLLEETDGG--RGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAFI 180
           MM TGTPLRVSL+RLLEETDGG       NLGVAE KR+E  NDSMCCVCMGRKKGAAFI
Sbjct: 121 MMETGTPLRVSLLRLLEETDGGCDXXXXXNLGVAEKKRDETRNDSMCCVCMGRKKGAAFI 180

Query: 181 PCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           PCGHTFCR+CSRELWLNRGSCPLCNRPIIEILDIF
Sbjct: 181 PCGHTFCRICSRELWLNRGSCPLCNRPIIEILDIF 215

[Close]

BLAST of Bhi03G000481 vs. NCBI nr
Match: XP_022977841.1 (uncharacterized protein LOC111478017 isoform X2 [Cucurbita maxima] >XP_022977842.1 uncharacterized protein LOC111478017 isoform X2 [Cucurbita maxima])

HSP 1 Score: 341.3 bits (874), Expect = 2.4e-90
Identity = 178/216 (82.41%), Postives = 187/216 (86.57%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRN-GXXXXXXRRVPDVEVTNT 60
           MDGVDGGGR+R SLKQRLGFKVMGCCGATWG R  T SVR   XXXXXX  VP+ EVTN 
Sbjct: 1   MDGVDGGGRRRQSLKQRLGFKVMGCCGATWGSRPATGSVRGXXXXXXXXXXVPETEVTNP 60

Query: 61  RR--EERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDS 120
           RR  EER+LDRSC+SPLSV SP  SGMNLATALAAERRLRASPRG EGG VE +NNDFDS
Sbjct: 61  RRFQEERQLDRSCMSPLSVPSPATSGMNLATALAAERRLRASPRGAEGGFVESSNNDFDS 120

Query: 121 LGGMMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAF 180
           LGGMM T TPL+VSL+RLL+ETDG  GG G LGVAE KREE GNDSMCCVCMGRKKGAAF
Sbjct: 121 LGGMMVTETPLKVSLIRLLKETDGSDGGRGILGVAEKKREEAGNDSMCCVCMGRKKGAAF 180

Query: 181 IPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           IPCGHTFCRVCSRELWLNRG CPLCNRPI+EILDIF
Sbjct: 181 IPCGHTFCRVCSRELWLNRGFCPLCNRPIMEILDIF 216

[Close]

BLAST of Bhi03G000481 vs. NCBI nr
Match: XP_022977839.1 (uncharacterized protein LOC111478017 isoform X1 [Cucurbita maxima])

HSP 1 Score: 341.3 bits (874), Expect = 2.4e-90
Identity = 178/216 (82.41%), Postives = 187/216 (86.57%), Query Frame = 0


Query: 1   MDGVDGGGRQRPSLKQRLGFKVMGCCGATWGFRSPTVSVRN-GXXXXXXRRVPDVEVTNT 60
           MDGVDGGGR+R SLKQRLGFKVMGCCGATWG R  T SVR   XXXXXX  VP+ EVTN 
Sbjct: 29  MDGVDGGGRRRQSLKQRLGFKVMGCCGATWGSRPATGSVRGXXXXXXXXXXVPETEVTNP 88

Query: 61  RR--EERELDRSCLSPLSVQSPGPSGMNLATALAAERRLRASPRGMEGGIVEFNNNDFDS 120
           RR  EER+LDRSC+SPLSV SP  SGMNLATALAAERRLRASPRG EGG VE +NNDFDS
Sbjct: 89  RRFQEERQLDRSCMSPLSVPSPATSGMNLATALAAERRLRASPRGAEGGFVESSNNDFDS 148

Query: 121 LGGMMGTGTPLRVSLMRLLEETDGGRGGCGNLGVAETKREEPGNDSMCCVCMGRKKGAAF 180
           LGGMM T TPL+VSL+RLL+ETDG  GG G LGVAE KREE GNDSMCCVCMGRKKGAAF
Sbjct: 149 LGGMMVTETPLKVSLIRLLKETDGSDGGRGILGVAEKKREEAGNDSMCCVCMGRKKGAAF 208

Query: 181 IPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214
           IPCGHTFCRVCSRELWLNRG CPLCNRPI+EILDIF
Sbjct: 209 IPCGHTFCRVCSRELWLNRGFCPLCNRPIMEILDIF 244

The following BLAST results are available for this feature:

BLAST of Bhi03G000481 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8LA32\|LUL4_ARATH	8.6e-07	40.35	Probable E3 ubiquitin-protein ligase LUL4 OS=Arabidopsis thaliana OX=3702 GN=LUL...	[more]
sp\|Q8BFW4\|TRI65_MOUSE	1.1e-04	40.00	Tripartite motif-containing protein 65 OS=Mus musculus OX=10090 GN=Trim65 PE=1 S...	[more]
sp\|Q8IUD6\|RN135_HUMAN	5.2e-04	39.58	E3 ubiquitin-protein ligase RNF135 OS=Homo sapiens OX=9606 GN=RNF135 PE=1 SV=2	[more]

BLAST of Bhi03G000481 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G03965.1	2.2e-45	47.35	RING/U-box superfamily protein	[more]
AT4G22250.1	9.6e-41	47.17	RING/U-box superfamily protein	[more]
AT1G62370.1	8.4e-29	40.55	RING/U-box superfamily protein	[more]
AT4G13100.2	3.3e-25	56.31	RING/U-box superfamily protein	[more]
AT3G25030.1	2.8e-24	57.95	RING/U-box superfamily protein	[more]

BLAST of Bhi03G000481 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3AZT0\|A0A1S3AZT0_CUCME	4.6e-106	89.20	uncharacterized protein LOC103484644 OS=Cucumis melo OX=3656 GN=LOC103484644 PE=...	[more]
tr\|A0A0A0KMY7\|A0A0A0KMY7_CUCSA	3.8e-100	84.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G512860 PE=4 SV=1	[more]
tr\|A0A2G9GM08\|A0A2G9GM08_9LAMI	3.1e-54	57.59	Putative E3 ubiquitin ligase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_21...	[more]
tr\|A0A061GHE7\|A0A061GHE7_THECC	7.0e-54	60.27	RING/U-box superfamily protein, putative isoform 1 OS=Theobroma cacao OX=3641 GN...	[more]
tr\|A0A2N9EQT2\|A0A2N9EQT2_FAGSY	1.5e-51	57.85	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5015 PE=4 SV=1	[more]

BLAST of Bhi03G000481 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008440052.1	7.0e-106	89.20	PREDICTED: uncharacterized protein LOC103484644 [Cucumis melo] >XP_008440053.1 P...	[more]
KGN49071.1	5.7e-100	84.65	hypothetical protein Csa_6G512860 [Cucumis sativus]	[more]
XP_004134982.2	5.7e-100	84.65	PREDICTED: uncharacterized protein LOC101211804 [Cucumis sativus]	[more]
XP_022977841.1	2.4e-90	82.41	uncharacterized protein LOC111478017 isoform X2 [Cucurbita maxima] >XP_022977842...	[more]
XP_022977839.1	2.4e-90	82.41	uncharacterized protein LOC111478017 isoform X1 [Cucurbita maxima]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013083	Znf_RING/FYVE/PHD
IPR001841	Znf_RING

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000481	Bhi03M000481	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 165..202 e-value: 2.8E-6 score: 36.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 165..203 score: 10.896
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 162..208 e-value: 1.8E-12 score: 46.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..69
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..83
None	No IPR available	PANTHER	PTHR12183	UNCHARACTERIZED RING ZINC FINGER-CONTAINING PROTEIN	coord: 1..213
None	No IPR available	PANTHER	PTHR12183:SF20	C3HC4 TYPE RING-FINGER PROTEIN	coord: 1..213
None	No IPR available	CDD	cd16449	RING-HC	coord: 165..202 e-value: 1.73201E-8 score: 47.4621
None	No IPR available	SUPERFAMILY	SSF57850	RING/U-box	coord: 157..210
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 149..213 e-value: 4.5E-14 score: 53.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi03G000481	Csa6G512860	Cucumber (Chinese Long) v2	cuwgoB628
Bhi03G000481	MELO3C007424	Melon (DHL92) v3.5.1	mewgoB635
Bhi03G000481	ClCG01G021600	Watermelon (Charleston Gray)	wcgwgoB227
Bhi03G000481	CSPI06G33180	Wild cucumber (PI 183967)	cpiwgoB634
Bhi03G000481	Cucsa.044890	Cucumber (Gy14) v1	cgywgoB026
Bhi03G000481	CmaCh07G003360	Cucurbita maxima (Rimu)	cmawgoB1058
Bhi03G000481	CmaCh03G005610	Cucurbita maxima (Rimu)	cmawgoB0788
Bhi03G000481	CmoCh07G003250	Cucurbita moschata (Rifu)	cmowgoB1051
Bhi03G000481	CmoCh03G005870	Cucurbita moschata (Rifu)	cmowgoB0781
Bhi03G000481	Lsi01G004640	Bottle gourd (USVL1VR-Ls)	lsiwgoB168
Bhi03G000481	Cp4.1LG10g09770	Cucurbita pepo (Zucchini)	cpewgoB0097
Bhi03G000481	Cp4.1LG19g08010	Cucurbita pepo (Zucchini)	cpewgoB0607
Bhi03G000481	CsGy6G032750	Cucumber (Gy14) v2	cgybwgoB598
Bhi03G000481	MELO3C007424.2	Melon (DHL92) v3.6.1	medwgoB641
Bhi03G000481	Carg13668	Silver-seed gourd	carwgoB1075
Bhi03G000481	Carg07864	Silver-seed gourd	carwgoB0759
Bhi03G000481	CsaV3_6G048640	Cucumber (Chinese Long) v3	cucwgoB648
Bhi03G000481	Cla014415	Watermelon (97103) v1	wgowmB432
Bhi03G000481	Cla97C01G021190	Watermelon (97103) v2	wgowmbB402

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi03G000481	Cucurbita maxima (Rimu)	cmawgoB0515
Bhi03G000481	Bottle gourd (USVL1VR-Ls)	lsiwgoB233
Bhi03G000481	Watermelon (97103) v2	wgowmbB401