Bhi12G000323 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G000323
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Subtilisin-like protease
Location	chr12: 10883915 .. 10885038 (+)
RNA-Seq Expression	Bhi12G000323
Synteny	Bhi12G000323

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATTAAGTTTTCAGGTACTTCCAACACAATCAAATTACAAACCTACATTGTCCATGTGCAGCAGCCAGAGCTCGGGATTTCGAATAACACCATTGATCTACAAAGTTGGTATACATCATTCTTACCAGAAACGATCGAAGCATCGAATGAACAGTCACGGTTACTTTATTTGTATCGTAACGTGATGAGTGGTTTTTCTGCAAGACTTACAGAAGAAGAAGTTAAAGCCATGGAAGAGAAGCAAGGTTTCATCTCAGCCAGACCAGAAACCATATTGAATTTGCACACAACTCATACACCCGAGTACTTGGGGTTGAACAAACAATTTGGATTGTGGAAAGATTCAAATTTTGGAAAGGGAGTTATCATAGGAGTGTTGGATACTGGAATTTCTCCTAAACATCCTTCGTTTAATGATGATGGAATGCCACAACCACCAGCTAAATGGAAAGGGAGATGCGAATTTGGTGCCTCCATCTGTAATAATAAGTTGATTGGTGCAAGAACTTTCAATCATGCTTATAGTGCTTTGATGGGGGAATCACCGATTGATGAACATGGACATGGCACACACACAGCAGGCACGGCTGCAGGCACTTTTGTTGAGGGTGCTGAAGCTTTGGGAAATGCAAGAGGGAAGGCAGTTGGAATGGCACCTCTAGCTCATCTTGCAATATATAAAGTTTGTACTCCAAAAGGCTGCTCAGATAGCGATGTACTTGCCGCATTAGATGCCGCAATTGATGACGGTGTCGACGTGCTCTCACTCTCCCTTGGCTCCTCTCCATGTCCGTTTTTCAATGACAGTATTGCTATAGGCTCATTTGCAGCCATTGAAAAGGGAATTTTTGTGAGTTGTTCAGCTGGGAATGAAGGCCCTTCCAAAAGCACAGTACGCAACGGAGCACCTTGGATTCTAACAGTCGGAGCAAGCACTATTGATCGAAAAATATCGGCACTATTGATCGAAAAATATCGGCATTAGCAAAGCTTGGAAATGGTGAAGTCCATGACGGTGAATCTTTGTTCCAGCCAAGAGATTTTGCATCAACATTCTTACCACTTGTATACGCTGGCAGCAGTGTAATCGAAAGGTCTAAATTTTGTGTTCATGGCTGA

mRNA sequence

ATGAAAATTAAGTTTTCAGGTACTTCCAACACAATCAAATTACAAACCTACATTGTCCATGTGCAGCAGCCAGAGCTCGGGATTTCGAATAACACCATTGATCTACAAAGTTGGTATACATCATTCTTACCAGAAACGATCGAAGCATCGAATGAACAGTCACGGTTACTTTATTTGTATCGTAACGTGATGAGTGGTTTTTCTGCAAGACTTACAGAAGAAGAAGTTAAAGCCATGGAAGAGAAGCAAGGTTTCATCTCAGCCAGACCAGAAACCATATTGAATTTGCACACAACTCATACACCCGAGTACTTGGGGTTGAACAAACAATTTGGATTGTGGAAAGATTCAAATTTTGGAAAGGGAGTTATCATAGGAGTGTTGGATACTGGAATTTCTCCTAAACATCCTTCGTTTAATGATGATGGAATGCCACAACCACCAGCTAAATGGAAAGGGAGATGCGAATTTGGTGCCTCCATCTGTAATAATAAGTTGATTGGTGCAAGAACTTTCAATCATGCTTATAGTGCTTTGATGGGGGAATCACCGATTGATGAACATGGACATGGCACACACACAGCAGGCACGGCTGCAGGCACTTTTGTTGAGGGTGCTGAAGCTTTGGGAAATGCAAGAGGGAAGGCAGTTGGAATGGCACCTCTAGCTCATCTTGCAATATATAAAGTTTGTACTCCAAAAGGCTGCTCAGATAGCGATGTACTTGCCGCATTAGATGCCGCAATTGATGACGGTGTCGACGTGCTCTCACTCTCCCTTGGCTCCTCTCCATGTCCGTTTTTCAATGACAGTATTGCTATAGGCTCATTTGCAGCCATTGAAAAGGGAATTTTTGTGAGTTGTTCAGCTGGGAATGAAGGCCCTTCCAAAAGCACACCAAGAGATTTTGCATCAACATTCTTACCACTTGTATACGCTGGCAGCAGTGTAATCGAAAGGTCTAAATTTTGTGTTCATGGCTGA

Coding sequence (CDS)

ATGAAAATTAAGTTTTCAGGTACTTCCAACACAATCAAATTACAAACCTACATTGTCCATGTGCAGCAGCCAGAGCTCGGGATTTCGAATAACACCATTGATCTACAAAGTTGGTATACATCATTCTTACCAGAAACGATCGAAGCATCGAATGAACAGTCACGGTTACTTTATTTGTATCGTAACGTGATGAGTGGTTTTTCTGCAAGACTTACAGAAGAAGAAGTTAAAGCCATGGAAGAGAAGCAAGGTTTCATCTCAGCCAGACCAGAAACCATATTGAATTTGCACACAACTCATACACCCGAGTACTTGGGGTTGAACAAACAATTTGGATTGTGGAAAGATTCAAATTTTGGAAAGGGAGTTATCATAGGAGTGTTGGATACTGGAATTTCTCCTAAACATCCTTCGTTTAATGATGATGGAATGCCACAACCACCAGCTAAATGGAAAGGGAGATGCGAATTTGGTGCCTCCATCTGTAATAATAAGTTGATTGGTGCAAGAACTTTCAATCATGCTTATAGTGCTTTGATGGGGGAATCACCGATTGATGAACATGGACATGGCACACACACAGCAGGCACGGCTGCAGGCACTTTTGTTGAGGGTGCTGAAGCTTTGGGAAATGCAAGAGGGAAGGCAGTTGGAATGGCACCTCTAGCTCATCTTGCAATATATAAAGTTTGTACTCCAAAAGGCTGCTCAGATAGCGATGTACTTGCCGCATTAGATGCCGCAATTGATGACGGTGTCGACGTGCTCTCACTCTCCCTTGGCTCCTCTCCATGTCCGTTTTTCAATGACAGTATTGCTATAGGCTCATTTGCAGCCATTGAAAAGGGAATTTTTGTGAGTTGTTCAGCTGGGAATGAAGGCCCTTCCAAAAGCACACCAAGAGATTTTGCATCAACATTCTTACCACTTGTATACGCTGGCAGCAGTGTAATCGAAAGGTCTAAATTTTGTGTTCATGGCTGA

Protein sequence

MKIKFSGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEASNEQSRLLYLYRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSALMGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDSDVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKSTPRDFASTFLPLVYAGSSVIERSKFCVHG

Homology

[Close]

BLAST of Bhi12G000323 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-71
Identity = 152/332 (45.78%), Postives = 201/332 (60.54%), Query Frame = 0


Query: 8   TSNTIKLQTYIVHVQ---QPELGISNNTIDLQSWYTSFLPETIEASNEQSRLLYLYRNVM 67
           +S++  L++YIVHVQ   +P L  S+N     +W+ S L  ++ +S + + LLY Y   +
Sbjct: 24  SSSSDGLESYIVHVQRSHKPSLFSSHN-----NWHVSLL-RSLPSSPQPATLLYSYSRAV 83

Query: 68  SGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKGVI 127
            GFSARL+  +  A+      IS  P+    +HTTHTP +LG ++  GLW +SN+G+ VI
Sbjct: 84  HGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVI 143

Query: 128 IGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFG----ASICNNKLIGARTFNHAY---- 187
           +GVLDTGI P+HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F   Y    
Sbjct: 144 VGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQR 203

Query: 188 ------SALMGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKV 247
                 +A    SP D  GHGTHTA TAAG+ V  A     ARG A GMA  A +A YK+
Sbjct: 204 NGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKI 263

Query: 248 CTPKGCSDSDVLAALDAAIDDGVDVLSLSLGSSPC--PFFNDSIAIGSFAAIEKGIFVSC 307
           C   GC DSD+LAA+D A+ DGV V+SLS+G+S     +  DSIAIG+F A   GI VSC
Sbjct: 264 CWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSC 323

Query: 308 SAGNEGPSKSTPRDFASTFLPLVYAGSSVIER 321
           SAGN GP+  T  + A   L +   G+S ++R
Sbjct: 324 SAGNSGPNPETATNIAPWILTV---GASTVDR 346

[Close]

BLAST of Bhi12G000323 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.6e-69
Identity = 146/316 (46.20%), Postives = 191/316 (60.44%), Query Frame = 0


Query: 6   SGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETI-----EASNEQSRLLYLY 65
           S +S  ++ QTYIV +  P    +        W+ SFL E +     E     SRLLY Y
Sbjct: 17  SSSSEILQKQTYIVQL-HPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSY 76

Query: 66  RNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLN--KQFGLWKDSN 125
            + + GF+A+LTE E + +      ++ RP+ +L + TT++ ++LGL+     G+W  S 
Sbjct: 77  GSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSR 136

Query: 126 FGKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCE----FGASICNNKLIGARTF-- 185
           FG+G IIGVLDTG+ P+ PSF+D GMP  P KWKG C+    F +S CN KLIGAR F  
Sbjct: 137 FGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIR 196

Query: 186 NHAYSALMGESP---------IDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLA 245
            H  +    ESP          D  GHGTHTA T  G+ V  A  LGN  G A GMAP A
Sbjct: 197 GHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGA 256

Query: 246 HLAIYKVCTPKGCSDSDVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKG 300
           H+A+YKVC   GC  SD+LAA+D AI D VDVLSLSLG  P P ++D+IAIG+F A+E+G
Sbjct: 257 HIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERG 316

[Close]

BLAST of Bhi12G000323 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 258.5 bits (659), Expect = 7.5e-69
Identity = 142/298 (47.65%), Postives = 191/298 (64.09%), Query Frame = 0


Query: 16  TYIVHVQQPELGISNNTIDLQS-WYTSFLPETIEASNEQSRLLYLYRNVMSGFSARLTEE 75
           TYIVH+ + ++    ++ DL S WY S    ++ + ++ + LLY Y N + GFS RLT+E
Sbjct: 31  TYIVHMAKSQM---PSSFDLHSNWYDS----SLRSISDSAELLYTYENAIHGFSTRLTQE 90

Query: 76  EVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFG-LWKDSNFGKGVIIGVLDTGIS 135
           E  ++  + G IS  PE    LHTT TP +LGL++    L+ ++     V++GVLDTG+ 
Sbjct: 91  EADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVW 150

Query: 136 PKHPSFNDDGMPQPPAKWKGRCE----FGASICNNKLIGARTFNHAYSALMG-------- 195
           P+  S++D+G    P+ WKG CE    F AS+CN KLIGAR F   Y + MG        
Sbjct: 151 PESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKES 210

Query: 196 ESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDSDV 255
            SP D+ GHGTHT+ TAAG+ VEGA  LG A G A GMAP A +A+YKVC   GC  SD+
Sbjct: 211 RSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDI 270

Query: 256 LAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST 300
           LAA+D AI D V+VLS+SLG     ++ D +AIG+FAA+E+GI VSCSAGN GPS S+
Sbjct: 271 LAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSS 321

[Close]

BLAST of Bhi12G000323 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.2e-66
Identity = 136/309 (44.01%), Postives = 191/309 (61.81%), Query Frame = 0


Query: 5   FSGTSNTIKLQTYIVHVQQPELGISNNTIDL-QSWYTSFLPETIEASN-EQSRLLYLYRN 64
           F  ++ + + QTY++H       ++ +T  +  S + S   E I   +     + Y+Y N
Sbjct: 32  FFASNVSSRKQTYVIHT------VTTSTKHIVTSLFNSLQTENINDDDFSLPEIHYIYEN 91

Query: 65  VMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKG 124
            MSGFSA LT++++  ++  +GFISA P+ +L+LHTT++ E+LGL    GLW +++    
Sbjct: 92  AMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSD 151

Query: 125 VIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCE----FGASICNNKLIGARTFNHAYSA 184
           VIIG++DTGISP+H SF D  M   P++W+G C+    F +S CN K+IGA  F   Y +
Sbjct: 152 VIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYES 211

Query: 185 LMGE--------SPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKV 244
           ++G+        S  D  GHGTHTA TAAG  V  A   G A+G A GM   + +A YK 
Sbjct: 212 IVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKA 271

Query: 245 CTPKGCSDSDVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSA 300
           C   GC+ +DV+AA+D AI DGVDV+SLSLG S  PF+ D IAI  F A++K IFVSCSA
Sbjct: 272 CWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSA 331

[Close]

BLAST of Bhi12G000323 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.6e-66
Identity = 142/312 (45.51%), Postives = 188/312 (60.26%), Query Frame = 0


Query: 10  NTIKLQTYIV---HVQQPELGISNNTIDLQSWYTSFLPETIEASNEQSRLLYLYRNVMSG 69
           +T   +TYI+   H  +PE  ++++      WYTS L       N +S LLY Y     G
Sbjct: 23  HTTAKKTYIIRVNHSDKPESFLTHH-----DWYTSQL-------NSESSLLYTYTTSFHG 82

Query: 70  FSARLTEEEVKA-MEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKGVII 129
           FSA L   E  + +      +    + +  LHTT TPE+LGLN +FG+    +   GVII
Sbjct: 83  FSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVII 142

Query: 130 GVLDTGISPKHPSFNDDGMPQPPAKWKGRCE----FGASICNNKLIGARTFNHAYSALMG 189
           GVLDTG+ P+  SF+D  MP+ P+KWKG CE    F + +CN KLIGAR+F+  +    G
Sbjct: 143 GVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASG 202

Query: 190 ---------ESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCT 249
                     SP D  GHGTHT+ TAAG+ V  A  LG A G A GMA  A +A YKVC 
Sbjct: 203 GGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCW 262

Query: 250 PKGCSDSDVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGN 305
             GC  SD+LAA+D AI DGVDVLSLSLG    P++ D+IAIG+F+A+E+G+FVSCSAGN
Sbjct: 263 STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGN 322

[Close]

BLAST of Bhi12G000323 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.3e-106
Identity = 197/363 (54.27%), Postives = 246/363 (67.77%), Query Frame = 0


Query: 8   TSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEASNEQSRLLYLYRNVMSGF 67
           ++ T   + YI+HV  PE  +   + DL+SWY SFLP T+ +S EQ R++Y Y+NV+ GF
Sbjct: 32  STETSSSKIYIIHVTGPEGKMLTESEDLESWYHSFLPPTLMSSEEQPRVIYSYKNVLRGF 91

Query: 68  SARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKGVIIGV 127
           +A LT+EE+ A+E+K GFISA P+ +L+  TTHTP++LGL +  G+WK+SNFGKGVIIGV
Sbjct: 92  AASLTQEELSAVEKKNGFISAHPQRVLHRQTTHTPKFLGLQQDTGVWKESNFGKGVIIGV 151

Query: 128 LDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSALMG---ESP 187
           LD+GI+P HPSF+D G+P PP KWKGRC+   + CNNKLIGAR FN A  A+ G   E+P
Sbjct: 152 LDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTACNNKLIGARAFNLAAEAMNGKKAEAP 211

Query: 188 IDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDSDVLAA 247
           IDE GHGTHTA TAAG FV  AE LGNA+G A GMAP AHLAIYKVC  + C +SD+LAA
Sbjct: 212 IDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAGMAPHAHLAIYKVCFGEDCPESDILAA 271

Query: 248 LDAAIDDGVDVLSLSLG-SSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST---- 307
           LDAA++DGVDV+S+SLG S P PFFNDS AIG+FAA++KGIFVSC+AGN GP  S+    
Sbjct: 272 LDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAFAAMQKGIFVSCAAGNSGPFNSSIVNA 331

Query: 308 -----------------------------------PRDFASTFLPLVYAGSSVIERSKFC 328
                                              P  F  T LPL YAG +  E S FC
Sbjct: 332 APWILTVGASTIDRRIVATAKLGNGQEFDGESVFQPSSFTPTLLPLAYAGKNGKEESAFC 391

[Close]

BLAST of Bhi12G000323 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.7e-102
Identity = 195/354 (55.08%), Postives = 236/354 (66.67%), Query Frame = 0


Query: 14  LQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEASNEQSRLLYLYRNVMSGFSARLTE 73
           LQTYIVHV +P+  +  N+ DL+S+Y SFLP T+  S   SR+++ Y +V +GF+A+L+ 
Sbjct: 26  LQTYIVHVDKPDAQVLANSADLESYYNSFLPATVSGSEVPSRIIHSYHHVATGFAAKLSG 85

Query: 74  EEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKGVIIGVLDTGIS 133
           EEVK ME+K GF+SA+ E +L LHTTHTP +LGL +  G W++SN+GKGVIIG+LDTGI+
Sbjct: 86  EEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMGFWQESNYGKGVIIGLLDTGIT 145

Query: 134 PKHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNHAYSALMGESPIDEHGHGT 193
           P HPSF+D  MP PPAKWKG+CEF G + CN K+IGAR F        G  P DE GHGT
Sbjct: 146 PGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARNFISG----SGVPPTDEEGHGT 205

Query: 194 HTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDSDVLAALDAAIDDG 253
           HTA TAAG FV  A   GNA G AVGMAPLAH+A+YKVC+  GCSD+D+LAALDAAIDDG
Sbjct: 206 HTASTAAGNFVNDANVFGNANGTAVGMAPLAHIAMYKVCSEDGCSDADILAALDAAIDDG 265

Query: 254 VDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST------------- 313
           VDVLSLSLG    PF+ D+IAIG+FAAI KGIFVS SAGN+GP  ST             
Sbjct: 266 VDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIFVSASAGNDGPLNSTLSNEAPWILTVGA 325

Query: 314 --------------------------PRDFASTFLPLVYAGSSVIERSKFCVHG 328
                                     P DF  T LPLVY G+S  E + FC  G
Sbjct: 326 STHDRKIVATAVLGNGQQYDGESAFQPADFPHTLLPLVYPGTS-DEEAAFCSSG 374

[Close]

BLAST of Bhi12G000323 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-93
Identity = 181/311 (58.20%), Postives = 218/311 (70.10%), Query Frame = 0


Query: 12  IKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEASNEQSRLLYLYRNVMSGFSARL 71
           I L TYIVHV++ E   S+ + DL SWY SFLP+T      + R+++ YR V SGF+ +L
Sbjct: 33  INLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFP---HKERMVFSYRKVASGFAVKL 92

Query: 72  TEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKGVIIGVLDTG 131
           T EE K+++EK   +SARPE  L LHTTHTP +LGL +  GLW D N GKGVIIG++DTG
Sbjct: 93  TPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTG 152

Query: 132 ISPKHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNHAYSALMGESPIDEHGH 191
           I P HPSFND+GMP PPAKWKG CEF G  +CNNKLIGAR  N   SA+  E P +   H
Sbjct: 153 IFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGAR--NLVKSAIQ-EPPFENFFH 212

Query: 192 GTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPK-GCSDSDVLAALDAAI 251
           GTHTA  AAG F+E A   GNA+G A GMAP AHLAIYKVC  K GC++S +LAA+D AI
Sbjct: 213 GTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMDIAI 272

Query: 252 DDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKSTPRDFASTFLP 311
           +DGVDVLSLSLG    PFF D IAIG+FAA + G+FVSCSA N GP  ST  + A   L 
Sbjct: 273 EDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPWILT 332

Query: 312 LVYAGSSVIER 321
           +   G+S I+R
Sbjct: 333 V---GASTIDR 334

[Close]

BLAST of Bhi12G000323 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.5e-93
Identity = 192/358 (53.63%), Postives = 229/358 (63.97%), Query Frame = 0


Query: 14  LQTYIVHVQQPEL-GISNNTIDLQSWYTSFLPETIEASNEQSRLLYLYRNVMSGFSARLT 73
           L TYIVHV++ E+ G   +T +L +W+ SFLPET    + + R+++ YRNV SGF+ RLT
Sbjct: 38  LTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPET----SNKDRMVFSYRNVASGFAVRLT 97

Query: 74  EEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKGVIIGVLDTGI 133
            EE  A++EK+  +S RPE  L+LHTTHTP +LGL +  GLW DSN GKGVIIGV+DTGI
Sbjct: 98  PEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTGI 157

Query: 134 SPKHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNHAYSALMGESPIDEHGHG 193
            P H SFND+GMP PPAKWKG CEF G S+CNNKLIGAR  N   SA+  E P ++  HG
Sbjct: 158 YPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-EPPYEDFFHG 217

Query: 194 THTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPK---GCSDSDVLAALDAA 253
           THTA  AAG FVEGA   GNARG A GMAP AHLAIYKVC+ K    C +S +LAA+D A
Sbjct: 218 THTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMDIA 277

Query: 254 IDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST--------- 313
           I+DGVDVLSLSLG    PFF D IAIG+FAA +KGIFVSCSA N GP  S+         
Sbjct: 278 IEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPWIL 337

Query: 314 ------------------------------PRDFASTFLPLVYAGSSVIERSKFCVHG 328
                                         P+DF+S  LPLVYA +     S  C  G
Sbjct: 338 TVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPG 388

[Close]

BLAST of Bhi12G000323 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.5e-93
Identity = 181/343 (52.77%), Postives = 230/343 (67.06%), Query Frame = 0


Query: 14  LQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEASNEQSRLLYLYRNVMSGFSARLTE 73
           L TYIVHV++P++  S+   DL ++Y S LPE+ + +N+  R+++ YRNV++GF+ +LT 
Sbjct: 42  LSTYIVHVRKPQVIQSD---DLHTFYYSLLPESTKTTNQ--RIVFTYRNVVNGFAVKLTP 101

Query: 74  EEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFGKGVIIGVLDTGIS 133
           EE KA+++ +  +SARPE IL+LHTTHTP +LGL +  GLWK SN GKGVIIG+LDTGIS
Sbjct: 102 EEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILDTGIS 161

Query: 134 PKHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNHAYSALMGESPIDEHGHGT 193
           P HPSF+D+GMP PPAKW G CEF G   CNNK+IGAR F    +  +   P D+ GHGT
Sbjct: 162 PFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLTL---PFDDVGHGT 221

Query: 194 HTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDSDVLAALDAAIDDG 253
           HTA TAAG  V+GA   GNA G AVGMAP AH+A+YKVC   GCS+S +LA +D A+DDG
Sbjct: 222 HTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDDG 281

Query: 254 VDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST------------- 313
           VDVLSLSLG    PFF D IA+G+F AI+KGIFVSCSA N GP+ S+             
Sbjct: 282 VDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVGA 341

Query: 314 --------------------------PRDFASTFLPLVYAGSS 317
                                     P+DFA + LPLVYAG++
Sbjct: 342 SSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGAN 376

[Close]

BLAST of Bhi12G000323 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 541.2 bits (1393), Expect = 6.2e-150
Identity = 278/366 (75.96%), Postives = 300/366 (81.97%), Query Frame = 0


Query: 1   MKIKFSGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEASNEQSRLLYLY 60
           MK + SGTSN IKLQTYIVHV++ EL I  + IDL+SWYTSFLPETI+ SNEQSRLLY +
Sbjct: 1   MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSF 60

Query: 61  RNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNFG 120
           RNVMSGFSARLT EEVKAMEEK+GFISARPETILNLHTTHTPEYLGLN+QFGLWKDSNFG
Sbjct: 61  RNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFG 120

Query: 121 KGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSALM 180
           KGVIIGVLDTGISP HPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFN A SALM
Sbjct: 121 KGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSALM 180

Query: 181 GESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDSD 240
           GESPIDE+GHGTHTA TAAGTFVEGAEALGNARGKAVGMAPLAHLA+YKVC+PKGCS SD
Sbjct: 181 GESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSD 240

Query: 241 VLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST- 300
           +LAALDAAI DGVDVLSLSLG+   PF+ D+IAIG+FAAI+ GIFVSCSAGN GPSKST 
Sbjct: 241 ILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTL 300

Query: 301 --------------------------------------PRDFASTFLPLVYAGSSVIERS 328
                                                 PRDF S+FLPLVYAGSS ++ S
Sbjct: 301 ANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGS 360

[Close]

BLAST of Bhi12G000323 vs. NCBI nr
Match: XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])

HSP 1 Score: 510.8 bits (1314), Expect = 9.0e-141
Identity = 261/367 (71.12%), Postives = 291/367 (79.29%), Query Frame = 0


Query: 1   MKIKFSGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEA-SNEQSRLLYL 60
           MK +    SNT  LQTYIVHV+QPE+ I  +TIDLQ+WYTSFLPETIEA SNEQSRLLY 
Sbjct: 21  MKTELWSASNT-NLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYS 80

Query: 61  YRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNF 120
           YR+V+SGFSARLT+E+VK MEEK GFISA PET LNLHTTHTPEYLGLN+ FGLWK+SNF
Sbjct: 81  YRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNF 140

Query: 121 GKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSAL 180
           GKGVIIGVLDTGI P HPSFND+GMP PPAKWKGRCEFGASICNNKLIGARTFN A +  
Sbjct: 141 GKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVS 200

Query: 181 MGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDS 240
           +G+SP DE+GHGTHTA TAAGTFV+GAEALGNARGKAVGMAPLAH+A+YKVC+PKGCS S
Sbjct: 201 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSS 260

Query: 241 DVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST 300
           D+LAALDAAIDDGVDVLSLSLG+   PFF D+IA+G+FAAI+KGIFVSCSAGN GPSK+T
Sbjct: 261 DILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNT 320

Query: 301 ---------------------------------------PRDFASTFLPLVYAGSSVIER 328
                                                  PRDF+S FLPLVYAG S IE 
Sbjct: 321 LANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEG 380

[Close]

BLAST of Bhi12G000323 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 505.4 bits (1300), Expect = 3.8e-139
Identity = 260/367 (70.84%), Postives = 290/367 (79.02%), Query Frame = 0


Query: 1   MKIKFSGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEA-SNEQSRLLYL 60
           MK + S TSNT  LQTYIVHV+QP L I  +TIDLQ+WYTSFLPETIEA S+EQ RLLY 
Sbjct: 21  MKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 80

Query: 61  YRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNF 120
           YR+VMSGFSARLT+E+VKAMEEK GFISA PET LNLHTTHTPEYLGLNK FGLWK+SNF
Sbjct: 81  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSNF 140

Query: 121 GKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSAL 180
           GKGVIIGVLDTGI P HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFN A + +
Sbjct: 141 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 200

Query: 181 MGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDS 240
           +G+SP DE+GHGTHTA TAAGTFV+GAEALGNARGKAVGMAPLAH+A+YKVC+P GCS S
Sbjct: 201 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 260

Query: 241 DVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST 300
           D+LAALDAAIDDGVDVLSLSLG+   PFF D+IA+G+FAAI+KGIFVSCSAGN GPSK+T
Sbjct: 261 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKNT 320

Query: 301 ---------------------------------------PRDFASTFLPLVYAGSSVIER 328
                                                  PR+F S FLPLVYAG S IE 
Sbjct: 321 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 380

[Close]

BLAST of Bhi12G000323 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 504.2 bits (1297), Expect = 8.4e-139
Identity = 260/367 (70.84%), Postives = 289/367 (78.75%), Query Frame = 0


Query: 1   MKIKFSGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEA-SNEQSRLLYL 60
           MK + S TSNT  LQTYIVHV+QP L I  +TIDLQ+WYTSFLPETIEA S+EQ RLLY 
Sbjct: 1   MKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 60

Query: 61  YRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNF 120
           YR+VMSGFSARLT+E+VKAMEEK GFISA PET LNLHTTHTPEYLGLN  FGLWK+SNF
Sbjct: 61  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 120

Query: 121 GKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSAL 180
           GKGVIIGVLDTGI P HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFN A + +
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 180

Query: 181 MGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDS 240
           +G+SP DE+GHGTHTA TAAGTFV+GAEALGNARGKAVGMAPLAH+A+YKVC+P GCS S
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 240

Query: 241 DVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST 300
           D+LAALDAAIDDGVDVLSLSLG+   PFF D+IAIG+FAAI+KGIFVSCSAGN GPSK+T
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 301 ---------------------------------------PRDFASTFLPLVYAGSSVIER 328
                                                  PR+F S FLPLVYAG S IE 
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 360

[Close]

BLAST of Bhi12G000323 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 436.0 bits (1120), Expect = 2.8e-118
Identity = 226/368 (61.41%), Postives = 263/368 (71.47%), Query Frame = 0


Query: 2   KIKFSGTSNTIKLQTYIVHVQQPEL---GISNNTIDLQSWYTSFLPETIEASNEQSRLLY 61
           K++   T    KLQTYIVHV+QP++   G S+N   ++SWY SF+P++ E + EQ +LLY
Sbjct: 22  KVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND-HIESWYMSFIPKSTETTVEQPQLLY 81

Query: 62  LYRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSN 121
            YRNVMSGFSARLT E+VKAME+K GFISA PETI++LHTTHTPEYLGLN+QFG+WK+SN
Sbjct: 82  SYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSN 141

Query: 122 FGKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSA 181
           FGKGVI+GVLDTGI P HPSFND+GM  PPAKWKG+CEF +S+CNNKLIGARTFN   + 
Sbjct: 142 FGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNF 201

Query: 182 LMGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSD 241
           LM ESP DE GHGTHTA TAAG FVE AEALGNA+GKA G+APLAHLAIYKVC+ K C  
Sbjct: 202 LMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPS 261

Query: 242 SDVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKS 301
           SDV A +DAAIDDGVDVLS+SLGS   PFF D+IA+ +F AI+KGIFVS SAGN GP  S
Sbjct: 262 SDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNS 321

Query: 302 T---------------------------------------PRDFASTFLPLVYAGSSVIE 328
           T                                       P DF S FLPLVYAG+   +
Sbjct: 322 TLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENK 381

[Close]

BLAST of Bhi12G000323 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.3e-141
Identity = 261/367 (71.12%), Postives = 291/367 (79.29%), Query Frame = 0


Query: 1   MKIKFSGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEA-SNEQSRLLYL 60
           MK +    SNT  LQTYIVHV+QPE+ I  +TIDLQ+WYTSFLPETIEA SNEQSRLLY 
Sbjct: 1   MKTELWSASNT-NLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYS 60

Query: 61  YRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNF 120
           YR+V+SGFSARLT+E+VK MEEK GFISA PET LNLHTTHTPEYLGLN+ FGLWK+SNF
Sbjct: 61  YRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNF 120

Query: 121 GKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSAL 180
           GKGVIIGVLDTGI P HPSFND+GMP PPAKWKGRCEFGASICNNKLIGARTFN A +  
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVS 180

Query: 181 MGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDS 240
           +G+SP DE+GHGTHTA TAAGTFV+GAEALGNARGKAVGMAPLAH+A+YKVC+PKGCS S
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSS 240

Query: 241 DVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST 300
           D+LAALDAAIDDGVDVLSLSLG+   PFF D+IA+G+FAAI+KGIFVSCSAGN GPSK+T
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 301 ---------------------------------------PRDFASTFLPLVYAGSSVIER 328
                                                  PRDF+S FLPLVYAG S IE 
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEG 360

[Close]

BLAST of Bhi12G000323 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.8e-139
Identity = 260/367 (70.84%), Postives = 290/367 (79.02%), Query Frame = 0


Query: 1   MKIKFSGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEA-SNEQSRLLYL 60
           MK + S TSNT  LQTYIVHV+QP L I  +TIDLQ+WYTSFLPETIEA S+EQ RLLY 
Sbjct: 21  MKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 80

Query: 61  YRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNF 120
           YR+VMSGFSARLT+E+VKAMEEK GFISA PET LNLHTTHTPEYLGLNK FGLWK+SNF
Sbjct: 81  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSNF 140

Query: 121 GKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSAL 180
           GKGVIIGVLDTGI P HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFN A + +
Sbjct: 141 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 200

Query: 181 MGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDS 240
           +G+SP DE+GHGTHTA TAAGTFV+GAEALGNARGKAVGMAPLAH+A+YKVC+P GCS S
Sbjct: 201 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 260

Query: 241 DVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST 300
           D+LAALDAAIDDGVDVLSLSLG+   PFF D+IA+G+FAAI+KGIFVSCSAGN GPSK+T
Sbjct: 261 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKNT 320

Query: 301 ---------------------------------------PRDFASTFLPLVYAGSSVIER 328
                                                  PR+F S FLPLVYAG S IE 
Sbjct: 321 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 380

[Close]

BLAST of Bhi12G000323 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 4.1e-139
Identity = 260/367 (70.84%), Postives = 289/367 (78.75%), Query Frame = 0


Query: 1   MKIKFSGTSNTIKLQTYIVHVQQPELGISNNTIDLQSWYTSFLPETIEA-SNEQSRLLYL 60
           MK + S TSNT  LQTYIVHV+QP L I  +TIDLQ+WYTSFLPETIEA S+EQ RLLY 
Sbjct: 1   MKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 60

Query: 61  YRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSNF 120
           YR+VMSGFSARLT+E+VKAMEEK GFISA PET LNLHTTHTPEYLGLN  FGLWK+SNF
Sbjct: 61  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 120

Query: 121 GKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSAL 180
           GKGVIIGVLDTGI P HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFN A + +
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 180

Query: 181 MGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSDS 240
           +G+SP DE+GHGTHTA TAAGTFV+GAEALGNARGKAVGMAPLAH+A+YKVC+P GCS S
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 240

Query: 241 DVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKST 300
           D+LAALDAAIDDGVDVLSLSLG+   PFF D+IAIG+FAAI+KGIFVSCSAGN GPSK+T
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 301 ---------------------------------------PRDFASTFLPLVYAGSSVIER 328
                                                  PR+F S FLPLVYAG S IE 
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 360

[Close]

BLAST of Bhi12G000323 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.4e-118
Identity = 226/368 (61.41%), Postives = 263/368 (71.47%), Query Frame = 0


Query: 2   KIKFSGTSNTIKLQTYIVHVQQPEL---GISNNTIDLQSWYTSFLPETIEASNEQSRLLY 61
           K++   T    KLQTYIVHV+QP++   G S+N   ++SWY SF+P++ E + EQ +LLY
Sbjct: 22  KVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND-HIESWYMSFIPKSTETTVEQPQLLY 81

Query: 62  LYRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSN 121
            YRNVMSGFSARLT E+VKAME+K GFISA PETI++LHTTHTPEYLGLN+QFG+WK+SN
Sbjct: 82  SYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSN 141

Query: 122 FGKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSA 181
           FGKGVI+GVLDTGI P HPSFND+GM  PPAKWKG+CEF +S+CNNKLIGARTFN   + 
Sbjct: 142 FGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNF 201

Query: 182 LMGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSD 241
           LM ESP DE GHGTHTA TAAG FVE AEALGNA+GKA G+APLAHLAIYKVC+ K C  
Sbjct: 202 LMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPS 261

Query: 242 SDVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKS 301
           SDV A +DAAIDDGVDVLS+SLGS   PFF D+IA+ +F AI+KGIFVS SAGN GP  S
Sbjct: 262 SDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNS 321

Query: 302 T---------------------------------------PRDFASTFLPLVYAGSSVIE 328
           T                                       P DF S FLPLVYAG+   +
Sbjct: 322 TLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENK 381

[Close]

BLAST of Bhi12G000323 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.4e-118
Identity = 226/368 (61.41%), Postives = 263/368 (71.47%), Query Frame = 0


Query: 2   KIKFSGTSNTIKLQTYIVHVQQPEL---GISNNTIDLQSWYTSFLPETIEASNEQSRLLY 61
           K++   T    KLQTYIVHV+QP++   G S+N   ++SWY SF+P++ E + EQ +LLY
Sbjct: 31  KVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND-HIESWYMSFIPKSTETTVEQPQLLY 90

Query: 62  LYRNVMSGFSARLTEEEVKAMEEKQGFISARPETILNLHTTHTPEYLGLNKQFGLWKDSN 121
            YRNVMSGFSARLT E+VKAME+K GFISA PETI++LHTTHTPEYLGLN+QFG+WK+SN
Sbjct: 91  SYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSN 150

Query: 122 FGKGVIIGVLDTGISPKHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNHAYSA 181
           FGKGVI+GVLDTGI P HPSFND+GM  PPAKWKG+CEF +S+CNNKLIGARTFN   + 
Sbjct: 151 FGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNF 210

Query: 182 LMGESPIDEHGHGTHTAGTAAGTFVEGAEALGNARGKAVGMAPLAHLAIYKVCTPKGCSD 241
           LM ESP DE GHGTHTA TAAG FVE AEALGNA+GKA G+APLAHLAIYKVC+ K C  
Sbjct: 211 LMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPS 270

Query: 242 SDVLAALDAAIDDGVDVLSLSLGSSPCPFFNDSIAIGSFAAIEKGIFVSCSAGNEGPSKS 301
           SDV A +DAAIDDGVDVLS+SLGS   PFF D+IA+ +F AI+KGIFVS SAGN GP  S
Sbjct: 271 SDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNS 330

Query: 302 T---------------------------------------PRDFASTFLPLVYAGSSVIE 328
           T                                       P DF S FLPLVYAG+   +
Sbjct: 331 TLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENK 390

The following BLAST results are available for this feature:

BLAST of Bhi12G000323 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14067.1	1.6e-71	45.78	Subtilase family protein	[more]
AT1G04110.1	2.6e-69	46.20	Subtilase family protein	[more]
AT5G67360.1	7.5e-69	47.65	Subtilase family protein	[more]
AT1G01900.1	1.2e-66	44.01	subtilase family protein	[more]
AT2G05920.1	1.6e-66	45.51	Subtilase family protein	[more]

BLAST of Bhi12G000323 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9QY38	3.3e-106	54.27	Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1	[more]
A0A0M3R8G2	1.7e-102	55.08	Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1	[more]
G7KEU7	1.1e-93	58.20	Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1	[more]
A9QY40	1.5e-93	53.63	Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1	[more]
A9QY39	1.5e-93	52.77	Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1	[more]

BLAST of Bhi12G000323 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906452.1	6.2e-150	75.96	subtilisin-like protease 4 [Benincasa hispida]	[more]
XP_004147599.2	9.0e-141	71.12	subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical pro...	[more]
XP_008437177.1	3.8e-139	70.84	PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo]	[more]
KAA0042822.1	8.4e-139	70.84	subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa]	[more]
KAA0042821.1	2.8e-118	61.41	subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa]	[more]

BLAST of Bhi12G000323 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KMQ7	4.3e-141	71.12	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1	[more]
A0A1S3AT16	1.8e-139	70.84	subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=...	[more]
A0A5A7THE4	4.1e-139	70.84	Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A5A7TMM4	1.4e-118	61.41	Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A1S3AUP1	1.4e-118	61.41	subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 186..199 score: 55.56 coord: 120..139 score: 44.36
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 16..97 e-value: 1.6E-9 score: 38.3
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 3..97 e-value: 1.7E-23 score: 84.9
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 120..301 e-value: 4.0E-29 score: 101.8
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 122..304 e-value: 1.8E-77 score: 263.1
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 96..306
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 11..308
None	No IPR available	PANTHER	PTHR10795:SF697	SUBTILISIN-LIKE PROTEASE SBT1.7	coord: 11..308
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 104..327 score: 22.972151
IPR023827	Peptidase S8, subtilisin, Asp-active site	PROSITE	PS00136	SUBTILASE_ASP	coord: 125..136
IPR034197	Cucumisin-like catalytic domain	CDD	cd04852	Peptidases_S8_3	coord: 94..299 e-value: 1.04022E-103 score: 304.521

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M000323	Bhi12M000323	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity