Bhi12G001211 (gene) Wax gourd (B227) v1

Overview
NameBhi12G001211
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionDNA helicase
Locationchr12: 45034282 .. 45034562 (-)
RNA-Seq ExpressionBhi12G001211
SyntenyBhi12G001211
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTGATTACCACGGGAGTATCGGCAAGCGAAAGGATGCGAAAGGAGAGTTTATTATCCGTAACTCGCAACATTTTTATCGAGAAAATGCAGCTTGGAGGACCCTCCATGTGTCTGACTGAGGTCTGCTGCTAACTTTTACTGTGTGCAATAACTTTACTTGTTAAGTTAAAGTTAAATTCTAAATCTTTCCAATTCTTCTGTAGTTGCTGGATGAGCTGAAGAAGAAGAATCTTAATAATGAAGTCCATCTCAACAATGTATATTACATCTCTTAA

mRNA sequence

ATGGATCTGATTACCACGGGAGTATCGGCAAGCGAAAGGATGCGAAAGGAGAGTTTATTATCCGTAACTCGCAACATTTTTATCGAGAAAATGCAGCTTGGAGGACCCTCCATGTGTCTGACTGAGTTGCTGGATGAGCTGAAGAAGAAGAATCTTAATAATGAAGTCCATCTCAACAATGTATATTACATCTCTTAA

Coding sequence (CDS)

ATGGATCTGATTACCACGGGAGTATCGGCAAGCGAAAGGATGCGAAAGGAGAGTTTATTATCCGTAACTCGCAACATTTTTATCGAGAAAATGCAGCTTGGAGGACCCTCCATGTGTCTGACTGAGTTGCTGGATGAGCTGAAGAAGAAGAATCTTAATAATGAAGTCCATCTCAACAATGTATATTACATCTCTTAA

Protein sequence

MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNNVYYIS
Homology
[Close]
BLAST of Bhi12G001211 vs. TAIR 10
Match: AT2G16440.1 (Minichromosome maintenance (MCM2/3/5) family protein )

HSP 1 Score: 73.6 bits (179), Expect = 6.8e-14
Identity = 39/63 (61.90%), Postives = 51/63 (80.95%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKK--NLNNEVHL 60
           MDLI TGVSASERMR+++  S  R+I +EKMQ+GG SM L+ELL+ELKK   N+N E+HL
Sbjct: 762 MDLINTGVSASERMRRDTFASSIRDIALEKMQIGGSSMRLSELLEELKKHGGNINTEIHL 821

Query: 61  NNV 62
           ++V
Sbjct: 822 HDV 824

[Close]
BLAST of Bhi12G001211 vs. ExPASy Swiss-Prot
Match: Q0WVF5 (DNA replication licensing factor MCM4 OS=Arabidopsis thaliana OX=3702 GN=MCM4 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 9.5e-13
Identity = 39/63 (61.90%), Postives = 51/63 (80.95%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKK--NLNNEVHL 60
           MDLI TGVSASERMR+++  S  R+I +EKMQ+GG SM L+ELL+ELKK   N+N E+HL
Sbjct: 762 MDLINTGVSASERMRRDTFASSIRDIALEKMQIGGSSMRLSELLEELKKHGGNINTEIHL 821

Query: 61  NNV 62
           ++V
Sbjct: 822 HDV 824

[Close]
BLAST of Bhi12G001211 vs. ExPASy Swiss-Prot
Match: Q5JKB0 (DNA replication licensing factor MCM4 OS=Oryza sativa subsp. japonica OX=39947 GN=MCM4 PE=3 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 8.1e-12
Identity = 34/61 (55.74%), Postives = 53/61 (86.89%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLI TG+SASER R+++L++ TRN+ +EKMQLGGPS+ + ELL+E++K++ + EVHL++
Sbjct: 780 MDLIMTGISASERQRRDNLVAATRNLVMEKMQLGGPSVRMIELLEEIRKQS-SMEVHLHD 839

Query: 61  V 62
           +
Sbjct: 840 L 839

[Close]
BLAST of Bhi12G001211 vs. NCBI nr
Match: XP_038887341.1 (DNA replication licensing factor MCM4 isoform X1 [Benincasa hispida])

HSP 1 Score: 99.8 bits (247), Expect = 9.5e-18
Identity = 51/61 (83.61%), Postives = 56/61 (91.80%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASERMR+ESLLS TRN+ +EKMQLGGPSM L ELLDELKKKN +NEVHLNN
Sbjct: 762 MDLITTGVSASERMRRESLLSATRNLIMEKMQLGGPSMRLAELLDELKKKNPDNEVHLNN 821

Query: 61  V 62
           +
Sbjct: 822 L 822

[Close]
BLAST of Bhi12G001211 vs. NCBI nr
Match: XP_008448069.1 (PREDICTED: DNA replication licensing factor MCM4 isoform X1 [Cucumis melo])

HSP 1 Score: 99.8 bits (247), Expect = 9.5e-18
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASER+R+ESLLS TRNI +EKMQLGGPSM L+ELLDELKKKN +NEVHLNN
Sbjct: 789 MDLITTGVSASERLRRESLLSATRNIIMEKMQLGGPSMRLSELLDELKKKNPDNEVHLNN 848

Query: 61  V 62
           +
Sbjct: 849 L 849

[Close]
BLAST of Bhi12G001211 vs. NCBI nr
Match: XP_008448070.1 (PREDICTED: DNA replication licensing factor MCM4 isoform X2 [Cucumis melo])

HSP 1 Score: 99.8 bits (247), Expect = 9.5e-18
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASER+R+ESLLS TRNI +EKMQLGGPSM L+ELLDELKKKN +NEVHLNN
Sbjct: 761 MDLITTGVSASERLRRESLLSATRNIIMEKMQLGGPSMRLSELLDELKKKNPDNEVHLNN 820

Query: 61  V 62
           +
Sbjct: 821 L 821

[Close]
BLAST of Bhi12G001211 vs. NCBI nr
Match: KAA0033785.1 (DNA replication licensing factor MCM4 isoform X2 [Cucumis melo var. makuwa] >TYK22349.1 DNA replication licensing factor MCM4 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 99.8 bits (247), Expect = 9.5e-18
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASER+R+ESLLS TRNI +EKMQLGGPSM L+ELLDELKKKN +NEVHLNN
Sbjct: 761 MDLITTGVSASERLRRESLLSATRNIIMEKMQLGGPSMRLSELLDELKKKNPDNEVHLNN 820

Query: 61  V 62
           +
Sbjct: 821 L 821

[Close]
BLAST of Bhi12G001211 vs. NCBI nr
Match: XP_022925017.1 (DNA replication licensing factor MCM4-like [Cucurbita moschata])

HSP 1 Score: 99.0 bits (245), Expect = 1.6e-17
Identity = 50/61 (81.97%), Postives = 57/61 (93.44%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASERMR+E++LS TRNI +EKMQLGGPSM LTELLDELKK+N +NEVHLNN
Sbjct: 762 MDLITTGVSASERMRRETILSATRNIIMEKMQLGGPSMRLTELLDELKKQNPDNEVHLNN 821

Query: 61  V 62
           +
Sbjct: 822 L 822

[Close]
BLAST of Bhi12G001211 vs. ExPASy TrEMBL
Match: A0A5A7SX80 (DNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1428G00670 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.6e-18
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASER+R+ESLLS TRNI +EKMQLGGPSM L+ELLDELKKKN +NEVHLNN
Sbjct: 761 MDLITTGVSASERLRRESLLSATRNIIMEKMQLGGPSMRLSELLDELKKKNPDNEVHLNN 820

Query: 61  V 62
           +
Sbjct: 821 L 821

[Close]
BLAST of Bhi12G001211 vs. ExPASy TrEMBL
Match: A0A1S3BJS1 (DNA helicase OS=Cucumis melo OX=3656 GN=LOC103490357 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.6e-18
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASER+R+ESLLS TRNI +EKMQLGGPSM L+ELLDELKKKN +NEVHLNN
Sbjct: 789 MDLITTGVSASERLRRESLLSATRNIIMEKMQLGGPSMRLSELLDELKKKNPDNEVHLNN 848

Query: 61  V 62
           +
Sbjct: 849 L 849

[Close]
BLAST of Bhi12G001211 vs. ExPASy TrEMBL
Match: A0A1S3BIA1 (DNA helicase OS=Cucumis melo OX=3656 GN=LOC103490357 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.6e-18
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASER+R+ESLLS TRNI +EKMQLGGPSM L+ELLDELKKKN +NEVHLNN
Sbjct: 761 MDLITTGVSASERLRRESLLSATRNIIMEKMQLGGPSMRLSELLDELKKKNPDNEVHLNN 820

Query: 61  V 62
           +
Sbjct: 821 L 821

[Close]
BLAST of Bhi12G001211 vs. ExPASy TrEMBL
Match: A0A6J1EAM6 (DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111432387 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 7.8e-18
Identity = 50/61 (81.97%), Postives = 57/61 (93.44%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVSASERMR+E++LS TRNI +EKMQLGGPSM LTELLDELKK+N +NEVHLNN
Sbjct: 762 MDLITTGVSASERMRRETILSATRNIIMEKMQLGGPSMRLTELLDELKKQNPDNEVHLNN 821

Query: 61  V 62
           +
Sbjct: 822 L 822

[Close]
BLAST of Bhi12G001211 vs. ExPASy TrEMBL
Match: A0A0A0K5P7 (DNA helicase OS=Cucumis sativus OX=3659 GN=Csa_7G004140 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-17
Identity = 50/61 (81.97%), Postives = 56/61 (91.80%), Query Frame = 0 

Query: 1   MDLITTGVSASERMRKESLLSVTRNIFIEKMQLGGPSMCLTELLDELKKKNLNNEVHLNN 60
           MDLITTGVS+SER+R+ESLLS TRNI +EKMQLGGPSM L+ELLDELKKKN  NEVHLNN
Sbjct: 761 MDLITTGVSSSERLRRESLLSATRNIIMEKMQLGGPSMRLSELLDELKKKNPENEVHLNN 820

Query: 61  V 62
           +
Sbjct: 821 L 821

The following BLAST results are available for this feature:
BLAST of Bhi12G001211 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G16440.16.8e-1461.90Minichromosome maintenance (MCM2/3/5) family protein [more]
BLAST of Bhi12G001211 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q0WVF59.5e-1361.90DNA replication licensing factor MCM4 OS=Arabidopsis thaliana OX=3702 GN=MCM4 PE... [more]
Q5JKB08.1e-1255.74DNA replication licensing factor MCM4 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
BLAST of Bhi12G001211 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038887341.19.5e-1883.61DNA replication licensing factor MCM4 isoform X1 [Benincasa hispida][more]
XP_008448069.19.5e-1883.61PREDICTED: DNA replication licensing factor MCM4 isoform X1 [Cucumis melo][more]
XP_008448070.19.5e-1883.61PREDICTED: DNA replication licensing factor MCM4 isoform X2 [Cucumis melo][more]
KAA0033785.19.5e-1883.61DNA replication licensing factor MCM4 isoform X2 [Cucumis melo var. makuwa] >TYK... [more]
XP_022925017.11.6e-1781.97DNA replication licensing factor MCM4-like [Cucurbita moschata][more]
BLAST of Bhi12G001211 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7SX804.6e-1883.61DNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1428G00670 ... [more]
A0A1S3BJS14.6e-1883.61DNA helicase OS=Cucumis melo OX=3656 GN=LOC103490357 PE=3 SV=1[more]
A0A1S3BIA14.6e-1883.61DNA helicase OS=Cucumis melo OX=3656 GN=LOC103490357 PE=3 SV=1[more]
A0A6J1EAM67.8e-1881.97DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111432387 PE=3 SV=1[more]
A0A0A0K5P71.3e-1781.97DNA helicase OS=Cucumis sativus OX=3659 GN=Csa_7G004140 PE=3 SV=1[more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M001211Bhi12M001211mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006271 DNA strand elongation involved in DNA replication
biological_process GO:0006268 DNA unwinding involved in DNA replication
biological_process GO:0000727 double-strand break repair via break-induced replication
biological_process GO:1902975 mitotic DNA replication initiation
biological_process GO:0009555 pollen development
cellular_component GO:0000785 chromatin
cellular_component GO:0042555 MCM complex
cellular_component GO:0000347 THO complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0017116 single-stranded DNA helicase activity