Bhi11G000385 (gene) Wax gourd (B227) v1

Overview
NameBhi11G000385
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCalcium-binding protein
Locationchr11: 11638369 .. 11641683 (+)
RNA-Seq ExpressionBhi11G000385
SyntenyBhi11G000385
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGACAAATGAGCATGAAGATGATGAAATGGGATGAGATGGAGAGAACATATTAAAAAGAGAGCGTCAAATCAGAAAACATTTGAGGAAGATTATGAAATAATTTTAAATTGATTGTTCCTATCTTTTAGGGAGATTGTCGAATTTGGAGCCGATACAGAAACGTGGTGGGTCGACCACAGAAATCGGTGAAAAGAATCCAATGAAGCTGCAGCAGCAAAGAATCATCATCATCCCCCATTTTCATCTTCATCTCACCAACAACTAATCTCTACCAAAATTTTCCTTTTCCTTCATTTATAAAAAACAAAACCCCCCTAGAAAAAATCTCTCGAAACACCCTTCGATTTCTCTTTCTTTTCCTTGTTGACTTTCTGAGATTCTTGAACAACAACAACAAGCGATTTTGGAACCAAGCTCAAGCTGTGCCTTAATCTCATACAGGTTAAACCTTCCTTCTTCCTTTCTTTTGACTTTTGTTTCATTGATTATGGTTTAGTTATTGGTTTAGAAGCTTTTGATTTATGGTTCTGTACTCTCCAGTGTTGAAATGTATATGGGCTTGTTTGGTAGTTTTGCTATCCTTTTTTGCTTGTTGGTTCATCTGTTTGTTGACCCCCCTTTTTTTTTTGGGATTAAATCTTAGTATTGAACCCTTTGTGGTTTTACTATGTATAATCTGATATAAGCAACCAAATTTAGGTCTTTTATTAACCCTTTTCTTCTTTTTAATTAGTAACTTGCAAACATTTATCTATGGTAAATATGAAGGGTTTTCCGGATGGATGACCCAAATTTGGGAGAAAAGATGAGATATGCCCCACTGAGGTTTTCTAATCCTTTGGTGACATTAAATTCGGATGAAATTATCAAAAAAAAACTCACTCTCTTACTCTTGCTATCTTATTCTCGCTCTTGCACCTTCATTCCTTTTCCTCTCACAGAACGCTCTCCTCTTTACCTTTTTCTCATGATCTCTCTCTCGCCCTTGCTTTTGCACTCCCGCTCTCTTACTCTTGCTTTTGCACTCTCGATCTGCAACTCAAACTGAATAAAGGGAGCGAAGAAGAAGACGGCTGGCAGCTGGAGTTGCTCGTGTGGACTTGTTAGAGCTGGTGCATGGTCTCATCGAGTTGGTCGCACGACAAGAAGAGCAACAAGAAAGAGAAGAAATAAAAGTAATTTTGTAAAATTGCTTGATGATGAGGTAAACAAAAGGTTGAAATTCTTAAGTTTTAAAAAGTGGGCAAAAATCATTTTGGGCCTCGAGTATGGTCTTTTAAATCAATTTTCCCAAATATATATGAAGGTAAACAACAGATGTTTAGAGTGAAGTTCGTTTTATGCAAGATTCAGTGTAATGTTTTAAAAGTTTAAAAGTAGAGATACTAAAGTAGAATATAGACCTTTTTCTTCCCTTTTTCTTTACGAAGTGATTCTTTATCCTATGTTTTTTTAATGGTGTAGACTGATTTGATTGTTTGTTTTATGGCTATGAACAGAGACATTGTTTAATTAGTTGGTTGATTCCAAAAACATCATCCCATTTTGTGTTGTCAAAGTTTTGTTGGCAATGGGGAGTGTGGTGGGAAAGTTGGAATCTCCAGATTGTGTACCAGAAACAAAACTTGAAGCCAAAATGGTTGAGGCAATGAGACACAGGGCATCTAAAGGAAGCATCATTAGGTCATTTGATAGCATAATCTTGAAATTCCCCAAAATTGATGATAGCCTTAGAAAATGCAAAACTATTTTCCAACAGTTTGGTGAGTACTTAGTTTGGTTCTCTTCTTTAGAATGCCAATTTAACTATATGGTTACTTACAGCCACAGCTCATTTGAACCTCAGTTTAAAAGTTCAAGTTTCAAGCCTTGAATTTACAATTACTCTACTTGTACCCCCACAAAATGGTGATGCTTAGAAGTTATGCCTTCTTTATAGATGAAGATTTGAACGGGATAATAGATCGTCGGGAGCTTAAGGAATGTTTTGATGGGCTGGAAATTTCGCTCACGGAGGAGGAAATCGATGATCTCTTTGATGCTTGTGATATTAGTACAGCTATGGGAATGAAGTTCAATGAATTCATTGTACTTCTCTGCCTTGTCTATCTTCTCAAGGATGATCCCAATGCTAAATTTTCTGTATCCGAACGTTTTGTTTTCAAGCTTCTTGTTTCTTCATTCTTTTTTCTCTATGCTTTTCTTTTGTATTCCCTGACTTCAACAGAAATCTCAATTCGGAATGCCAAAATTGGAGGAGACATTTGAATCTTTGGTCGATGCATTTGCGTTCTTGGACAAGAATAAGGATGGATATGTAAGCAAGAGCGAGATGATATCTGCAATAAACGAGACCACATCGGGAGAACGCTCTTCAGGGCGGATAGCGATGAGGAGATTTGGTAATGCTCATCGACCTTGTAGTCTTTCCATTTGTATATCGATTGCTGCATCAACGAATTAATGGTTGAGCTTGTTCACTTATTTGATCTTGCAGAGGAGATGGACTGGGACAAAAATGGAATGGTAAACTTTAAAGAATTTCTTTTCGCTTTCACCCATTGGGTTGGAATCGACGAGAATGAGGACGGAGAGGAAGAAGAGGAGGAATGAAACATGAAAAACATGTTATCTTGAATATGAAACTTCTTTTTCTAATTCTTCATTTGACGATGGGGAAACCGTGGTGCAGTAATGAAAGTAGGGATGCAGTTTCATCTGCACTTTTGTTGGCCTTCTCACACAAAATTGAACTAGAAGATAACAATGGTTTCAATGCATTGGTTGTAAATACTTGTGTGTACTGTGAAGAGTAGAAGTCATATAATTTGAAATGCTGGTGTAGAAGCCAAGCCTGAGCCTTTTTCATGTGGGGATTGTTTTTGTTTATTATTATTCTCCAATGGCAGCTTGGACTGATTTTCACTGGATGGCTTGAGAAAAGCTGTATTTTCTTTGGACTTGTCTGTCTTGCTCTCAATATTATAGACTTCTTTTCTTTTATTCAAAGGGAAATTTCACCTCATAGGCTATGAGTTGCTTGATCTTTCCTCTTTTGTTTGCTTGGCTCTTGTTTGGTTCTTTACCTCTTTTGTTTTTTGGCCTATTTGGTTGAAGATTTTAAGAGGGAGAAACAGTGTGAAAG

mRNA sequence

TGGACAAATGAGCATGAAGATGATGAAATGGGATGAGATGGAGAGAACATATTAAAAAGAGAGCGTCAAATCAGAAAACATTTGAGGAAGATTATGAAATAATTTTAAATTGATTGTTCCTATCTTTTAGGGAGATTGTCGAATTTGGAGCCGATACAGAAACGTGGTGGGTCGACCACAGAAATCGGTGAAAAGAATCCAATGAAGCTGCAGCAGCAAAGAATCATCATCATCCCCCATTTTCATCTTCATCTCACCAACAACTAATCTCTACCAAAATTTTCCTTTTCCTTCATTTATAAAAAACAAAACCCCCCTAGAAAAAATCTCTCGAAACACCCTTCGATTTCTCTTTCTTTTCCTTGTTGACTTTCTGAGATTCTTGAACAACAACAACAAGCGATTTTGGAACCAAGCTCAAGCTGTGCCTTAATCTCATACAGAACGCTCTCCTCTTTACCTTTTTCTCATGATCTCTCTCTCGCCCTTGCTTTTGCACTCCCGCTCTCTTACTCTTGCTTTTGCACTCTCGATCTGCAACTCAAACTGAATAAAGGGAGCGAAGAAGAAGACGGCTGGCAGCTGGAGTTGCTCGTGTGGACTTGTTAGAGCTGGTGCATGGTCTCATCGAGTTGGTCGCACGACAAGAAGAGCAACAAGAAAGAGAAGAAATAAAAGTAATTTTGTAAAATTGCTTGATGATGAGAGACATTGTTTAATTAGTTGGTTGATTCCAAAAACATCATCCCATTTTGTGTTGTCAAAGTTTTGTTGGCAATGGGGAGTGTGGTGGGAAAGTTGGAATCTCCAGATTGTGTACCAGAAACAAAACTTGAAGCCAAAATGGTTGAGGCAATGAGACACAGGGCATCTAAAGGAAGCATCATTAGGTCATTTGATAGCATAATCTTGAAATTCCCCAAAATTGATGATAGCCTTAGAAAATGCAAAACTATTTTCCAACAGTTTGATGAAGATTTGAACGGGATAATAGATCGTCGGGAGCTTAAGGAATGTTTTGATGGGCTGGAAATTTCGCTCACGGAGGAGGAAATCGATGATCTCTTTGATGCTTGTGATATTAGTACAGCTATGGGAATGAAGTTCAATGAATTCATTGTACTTCTCTGCCTTGTCTATCTTCTCAAGGATGATCCCAATGCTAAATTTTCTAAATCTCAATTCGGAATGCCAAAATTGGAGGAGACATTTGAATCTTTGGTCGATGCATTTGCGTTCTTGGACAAGAATAAGGATGGATATGTAAGCAAGAGCGAGATGATATCTGCAATAAACGAGACCACATCGGGAGAACGCTCTTCAGGGCGGATAGCGATGAGGAGATTTGAGGAGATGGACTGGGACAAAAATGGAATGGTAAACTTTAAAGAATTTCTTTTCGCTTTCACCCATTGGGTTGGAATCGACGAGAATGAGGACGGAGAGGAAGAAGAGGAGGAATGAAACATGAAAAACATGTTATCTTGAATATGAAACTTCTTTTTCTAATTCTTCATTTGACGATGGGGAAACCGTGGTGCAGTAATGAAAGTAGGGATGCAGTTTCATCTGCACTTTTGTTGGCCTTCTCACACAAAATTGAACTAGAAGATAACAATGGTTTCAATGCATTGGTTGTAAATACTTGTGTGTACTGTGAAGAGTAGAAGTCATATAATTTGAAATGCTGGTGTAGAAGCCAAGCCTGAGCCTTTTTCATGTGGGGATTGTTTTTGTTTATTATTATTCTCCAATGGCAGCTTGGACTGATTTTCACTGGATGGCTTGAGAAAAGCTGTATTTTCTTTGGACTTGTCTGTCTTGCTCTCAATATTATAGACTTCTTTTCTTTTATTCAAAGGGAAATTTCACCTCATAGGCTATGAGTTGCTTGATCTTTCCTCTTTTGTTTGCTTGGCTCTTGTTTGGTTCTTTACCTCTTTTGTTTTTTGGCCTATTTGGTTGAAGATTTTAAGAGGGAGAAACAGTGTGAAAG

Coding sequence (CDS)

ATGGGGAGTGTGGTGGGAAAGTTGGAATCTCCAGATTGTGTACCAGAAACAAAACTTGAAGCCAAAATGGTTGAGGCAATGAGACACAGGGCATCTAAAGGAAGCATCATTAGGTCATTTGATAGCATAATCTTGAAATTCCCCAAAATTGATGATAGCCTTAGAAAATGCAAAACTATTTTCCAACAGTTTGATGAAGATTTGAACGGGATAATAGATCGTCGGGAGCTTAAGGAATGTTTTGATGGGCTGGAAATTTCGCTCACGGAGGAGGAAATCGATGATCTCTTTGATGCTTGTGATATTAGTACAGCTATGGGAATGAAGTTCAATGAATTCATTGTACTTCTCTGCCTTGTCTATCTTCTCAAGGATGATCCCAATGCTAAATTTTCTAAATCTCAATTCGGAATGCCAAAATTGGAGGAGACATTTGAATCTTTGGTCGATGCATTTGCGTTCTTGGACAAGAATAAGGATGGATATGTAAGCAAGAGCGAGATGATATCTGCAATAAACGAGACCACATCGGGAGAACGCTCTTCAGGGCGGATAGCGATGAGGAGATTTGAGGAGATGGACTGGGACAAAAATGGAATGGTAAACTTTAAAGAATTTCTTTTCGCTTTCACCCATTGGGTTGGAATCGACGAGAATGAGGACGGAGAGGAAGAAGAGGAGGAATGA

Protein sequence

MGSVVGKLESPDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKTIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEEEE
Homology
BLAST of Bhi11G000385 vs. TAIR 10
Match: AT4G26470.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 309.3 bits (791), Expect = 2.6e-84
Identity = 158/228 (69.30%), Postives = 183/228 (80.26%), Query Frame = 0

Query: 1   MGSVVGKLES--PDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCK 60
           MG  V K E+   + VPETKLEAK++EA++ RAS+G+ ++SF+SI+LKFPKIDD LR CK
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  TIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLC 120
            IFQ+FDED NG ID  ELK C   LEIS  EEEI+DLF ACDI+  MG+ F EFIVLLC
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSG 180
           LVYLLKDD +    K   GMPKLE TFE+LVD F FLD+NKDGYVS+ EM+ AI+E  SG
Sbjct: 121 LVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--SG 180

Query: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEE 227
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFT WVGIDENE+ EEE+
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTQWVGIDENEEEEEED 226

BLAST of Bhi11G000385 vs. TAIR 10
Match: AT4G26470.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 241.9 bits (616), Expect = 5.0e-64
Identity = 126/192 (65.62%), Postives = 149/192 (77.60%), Query Frame = 0

Query: 1   MGSVVGKLES--PDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCK 60
           MG  V K E+   + VPETKLEAK++EA++ RAS+G+ ++SF+SI+LKFPKIDD LR CK
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  TIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLC 120
            IFQ+FDED NG ID  ELK C   LEIS  EEEI+DLF ACDI+  MG+ F EFIVLLC
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSG 180
           LVYLLKDD +    K   GMPKLE TFE+LVD F FLD+NKDGYVS+ EM+ AI+E  SG
Sbjct: 121 LVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--SG 180

Query: 181 ERSSGRIAMRRF 191
           ERSSGRIAM+RF
Sbjct: 181 ERSSGRIAMKRF 190

BLAST of Bhi11G000385 vs. TAIR 10
Match: AT3G24110.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 179.5 bits (454), Expect = 3.1e-45
Identity = 92/208 (44.23%), Postives = 137/208 (65.87%), Query Frame = 0

Query: 16  ETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKTIFQQFDEDLNGIIDRR 75
           + KL  KMVE+        S ++S DSII+KFPK+ + LR  +++F+ +D D NG ID  
Sbjct: 18  DAKLARKMVESRSIYPGHRS-LKSMDSIIMKFPKLREGLRNIRSVFESYDNDTNGTIDIE 77

Query: 76  ELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAKFSKSQ 135
           ELK+C + L++SL++EE+  L+  CD+  + G++FNEFIVLLCL+YLL    +   ++S+
Sbjct: 78  ELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLAKPSSESSTESR 137

Query: 136 FGMPKL-EETFESLVDAFAFLDKNKDGYVSKSEMISAI-NETTSGERSSGRIAMRRFEEM 195
              PKL E  F+ +V+ F FLDK+  G ++K+++I  + NE    ERS   +   RFEEM
Sbjct: 138 EMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYPLERSPSHVTNMRFEEM 197

Query: 196 DWDKNGMVNFKEFLFAFTHWVGIDENED 222
           DW + G V F+EFLFAF  WVG+D+ +D
Sbjct: 198 DWGRKGKVGFREFLFAFMSWVGLDDADD 224

BLAST of Bhi11G000385 vs. TAIR 10
Match: AT4G21940.1 (calcium-dependent protein kinase 15 )

HSP 1 Score: 60.5 bits (145), Expect = 2.1e-09
Identity = 42/156 (26.92%), Postives = 74/156 (47.44%), Query Frame = 0

Query: 51  DDSLRKCKTIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKF 110
           ++ ++  KT+F   D D +G I   ELK     L   LTE E+  L +A D+     + +
Sbjct: 401 EEEIKGLKTMFANMDTDKSGTITYEELKNGLAKLGSKLTEAEVKQLMEAADVDGNGTIDY 460

Query: 111 NEFIVLLCLVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMIS 170
            EFI             +A   + +F      +  E +  AF + DK+  G+++  E+ S
Sbjct: 461 IEFI-------------SATMHRYRF------DRDEHVFKAFQYFDKDNSGFITMDELES 520

Query: 171 AINETTSGERSSGRIAMRRFEEMDWDKNGMVNFKEF 207
           A+ E   G+ +S +  +    E+D D +G +N++EF
Sbjct: 521 AMKEYGMGDEASIKEVI---AEVDTDNDGRINYEEF 534

BLAST of Bhi11G000385 vs. TAIR 10
Match: AT4G21940.2 (calcium-dependent protein kinase 15 )

HSP 1 Score: 60.5 bits (145), Expect = 2.1e-09
Identity = 42/156 (26.92%), Postives = 74/156 (47.44%), Query Frame = 0

Query: 51  DDSLRKCKTIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKF 110
           ++ ++  KT+F   D D +G I   ELK     L   LTE E+  L +A D+     + +
Sbjct: 401 EEEIKGLKTMFANMDTDKSGTITYEELKNGLAKLGSKLTEAEVKQLMEAADVDGNGTIDY 460

Query: 111 NEFIVLLCLVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMIS 170
            EFI             +A   + +F      +  E +  AF + DK+  G+++  E+ S
Sbjct: 461 IEFI-------------SATMHRYRF------DRDEHVFKAFQYFDKDNSGFITMDELES 520

Query: 171 AINETTSGERSSGRIAMRRFEEMDWDKNGMVNFKEF 207
           A+ E   G+ +S +  +    E+D D +G +N++EF
Sbjct: 521 AMKEYGMGDEASIKEVI---AEVDTDNDGRINYEEF 534

BLAST of Bhi11G000385 vs. ExPASy Swiss-Prot
Match: Q52K82 (Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 3.6e-83
Identity = 158/228 (69.30%), Postives = 183/228 (80.26%), Query Frame = 0

Query: 1   MGSVVGKLES--PDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCK 60
           MG  V K E+   + VPETKLEAK++EA++ RAS+G+ ++SF+SI+LKFPKIDD LR CK
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  TIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLC 120
            IFQ+FDED NG ID  ELK C   LEIS  EEEI+DLF ACDI+  MG+ F EFIVLLC
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSG 180
           LVYLLKDD +    K   GMPKLE TFE+LVD F FLD+NKDGYVS+ EM+ AI+E  SG
Sbjct: 121 LVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--SG 180

Query: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEE 227
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFT WVGIDENE+ EEE+
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTQWVGIDENEEEEEED 226

BLAST of Bhi11G000385 vs. ExPASy Swiss-Prot
Match: Q9LRN6 (Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.3e-44
Identity = 92/208 (44.23%), Postives = 137/208 (65.87%), Query Frame = 0

Query: 16  ETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKTIFQQFDEDLNGIIDRR 75
           + KL  KMVE+        S ++S DSII+KFPK+ + LR  +++F+ +D D NG ID  
Sbjct: 18  DAKLARKMVESRSIYPGHRS-LKSMDSIIMKFPKLREGLRNIRSVFESYDNDTNGTIDIE 77

Query: 76  ELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLVYLLKDDPNAKFSKSQ 135
           ELK+C + L++SL++EE+  L+  CD+  + G++FNEFIVLLCL+YLL    +   ++S+
Sbjct: 78  ELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLAKPSSESSTESR 137

Query: 136 FGMPKL-EETFESLVDAFAFLDKNKDGYVSKSEMISAI-NETTSGERSSGRIAMRRFEEM 195
              PKL E  F+ +V+ F FLDK+  G ++K+++I  + NE    ERS   +   RFEEM
Sbjct: 138 EMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYPLERSPSHVTNMRFEEM 197

Query: 196 DWDKNGMVNFKEFLFAFTHWVGIDENED 222
           DW + G V F+EFLFAF  WVG+D+ +D
Sbjct: 198 DWGRKGKVGFREFLFAFMSWVGLDDADD 224

BLAST of Bhi11G000385 vs. ExPASy Swiss-Prot
Match: O49717 (Calcium-dependent protein kinase 15 OS=Arabidopsis thaliana OX=3702 GN=CPK15 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.9e-08
Identity = 42/156 (26.92%), Postives = 74/156 (47.44%), Query Frame = 0

Query: 51  DDSLRKCKTIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKF 110
           ++ ++  KT+F   D D +G I   ELK     L   LTE E+  L +A D+     + +
Sbjct: 401 EEEIKGLKTMFANMDTDKSGTITYEELKNGLAKLGSKLTEAEVKQLMEAADVDGNGTIDY 460

Query: 111 NEFIVLLCLVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMIS 170
            EFI             +A   + +F      +  E +  AF + DK+  G+++  E+ S
Sbjct: 461 IEFI-------------SATMHRYRF------DRDEHVFKAFQYFDKDNSGFITMDELES 520

Query: 171 AINETTSGERSSGRIAMRRFEEMDWDKNGMVNFKEF 207
           A+ E   G+ +S +  +    E+D D +G +N++EF
Sbjct: 521 AMKEYGMGDEASIKEVI---AEVDTDNDGRINYEEF 534

BLAST of Bhi11G000385 vs. ExPASy Swiss-Prot
Match: Q9ZSA2 (Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.8e-08
Identity = 43/156 (27.56%), Postives = 71/156 (45.51%), Query Frame = 0

Query: 51  DDSLRKCKTIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKF 110
           ++ ++  KT+F   D D +G I   ELK     L   L+E E+  L +A D+     + +
Sbjct: 379 EEEIKGLKTMFANIDTDKSGTITYEELKTGLTRLGSRLSETEVKQLMEAADVDGNGTIDY 438

Query: 111 NEFIVLLCLVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMIS 170
            EFI      Y L  D                   E +  AF   DK+  G++++ E+ S
Sbjct: 439 YEFISATMHRYKLDRD-------------------EHVYKAFQHFDKDNSGHITRDELES 498

Query: 171 AINETTSGERSSGRIAMRRFEEMDWDKNGMVNFKEF 207
           A+ E   G+ +S +  +    E+D D +G +NF+EF
Sbjct: 499 AMKEYGMGDEASIKEVI---SEVDTDNDGRINFEEF 512

BLAST of Bhi11G000385 vs. ExPASy Swiss-Prot
Match: Q9M101 (Calcium-dependent protein kinase 23 OS=Arabidopsis thaliana OX=3702 GN=CPK23 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.8e-08
Identity = 42/156 (26.92%), Postives = 74/156 (47.44%), Query Frame = 0

Query: 51  DDSLRKCKTIFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKF 110
           ++ ++  KT+F   D + +G I   +L+     L   L+E E+  L +A D+     + +
Sbjct: 368 EEEIKGLKTLFANMDTNRSGTITYEQLQTGLSRLRSRLSETEVQQLVEASDVDGNGTIDY 427

Query: 111 NEFIVLLCLVYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMIS 170
            EFI      Y L  D                   E +  AF  LDK+K+G++++ E+ S
Sbjct: 428 YEFISATMHRYKLHHD-------------------EHVHKAFQHLDKDKNGHITRDELES 487

Query: 171 AINETTSGERSSGRIAMRRFEEMDWDKNGMVNFKEF 207
           A+ E   G+ +S +  +    E+D D +G +NF+EF
Sbjct: 488 AMKEYGMGDEASIKEVI---SEVDTDNDGKINFEEF 501

BLAST of Bhi11G000385 vs. NCBI nr
Match: XP_038905190.1 (probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905191.1 probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905192.1 probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905193.1 probable calcium-binding protein CML21 [Benincasa hispida])

HSP 1 Score: 454.1 bits (1167), Expect = 6.9e-124
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0

Query: 1   MGSVVGKLESPDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKTI 60
           MGSVVGKLESPDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKTI
Sbjct: 1   MGSVVGKLESPDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKTI 60

Query: 61  FQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLV 120
           FQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLV
Sbjct: 61  FQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCLV 120

Query: 121 YLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGER 180
           YLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGER
Sbjct: 121 YLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGER 180

Query: 181 SSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEEEE 229
           SSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEEEE
Sbjct: 181 SSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEEEE 228

BLAST of Bhi11G000385 vs. NCBI nr
Match: XP_004149571.1 (probable calcium-binding protein CML21 [Cucumis sativus] >KGN56046.1 hypothetical protein Csa_009502 [Cucumis sativus])

HSP 1 Score: 424.5 bits (1090), Expect = 5.9e-115
Identity = 214/229 (93.45%), Postives = 220/229 (96.07%), Query Frame = 0

Query: 1   MGSVVGKLESP-DCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGSVVGKLESP +CVPETKLEAKMVE M+ RA+KGSIIRSFD IILKFPKIDDSLR CKT
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELK+CFDGLEI LTEEEIDDLFDACDISTAMGMKFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180
           VYLLKDDPNA FSKSQFGMPKLE+TFESLVDAF FLDKNKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEEEE 229
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFT WVGIDENEDGEEEEEE
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE 229

BLAST of Bhi11G000385 vs. NCBI nr
Match: XP_008449066.1 (PREDICTED: probable calcium-binding protein CML21 [Cucumis melo])

HSP 1 Score: 407.9 bits (1047), Expect = 5.7e-110
Identity = 205/224 (91.52%), Postives = 213/224 (95.09%), Query Frame = 0

Query: 1   MGSVVGKLESP-DCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGSVVGKLESP +CVPETKLEAKMVE MR RA+KGSIIRSFD I+LKFPKIDDSLR CKT
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMRRRATKGSIIRSFDCILLKFPKIDDSLRNCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELK+CFDGLEI LTEEEIDDLF+ACDIS AMG+KFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISAAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180
           VYLLKDDP+A FSKSQFGMPKLEETFESLVDAF FLDKNKDGYVSKSEMISAINETTSGE
Sbjct: 121 VYLLKDDPDAVFSKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGE 224
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFT WVGIDENE+ E
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEEEE 224

BLAST of Bhi11G000385 vs. NCBI nr
Match: XP_023540519.1 (probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540520.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540521.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540522.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 405.2 bits (1040), Expect = 3.7e-109
Identity = 208/228 (91.23%), Postives = 214/228 (93.86%), Query Frame = 0

Query: 1   MGSVVGKLES-PDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGSVVGKLES  +CVPETKLEAKMVEAMR RASKGSIIRSFD IILKFPKID SLRKCKT
Sbjct: 1   MGSVVGKLESHSECVPETKLEAKMVEAMRQRASKGSIIRSFDCIILKFPKIDGSLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IF++FDED NGIIDRRELKECFDGLEISLT+EEIDDLFDACD+STAMG+KFNEFIVLLCL
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180
           VYLLKDDPN    KSQFGMPKLEETFESLVDAF FLD NKDGYVSKSEMISAINETTSGE
Sbjct: 121 VYLLKDDPN---HKSQFGMPKLEETFESLVDAFVFLDANKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEEE 228
           RSSGRI MRRFEEMDWDKNGMVNFKEFLFAFT WVGIDENEDGEEEEE
Sbjct: 181 RSSGRIGMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEE 225

BLAST of Bhi11G000385 vs. NCBI nr
Match: XP_023005531.1 (probable calcium-binding protein CML21 [Cucurbita maxima] >XP_023005532.1 probable calcium-binding protein CML21 [Cucurbita maxima] >XP_023005533.1 probable calcium-binding protein CML21 [Cucurbita maxima])

HSP 1 Score: 401.7 bits (1031), Expect = 4.1e-108
Identity = 205/227 (90.31%), Postives = 213/227 (93.83%), Query Frame = 0

Query: 1   MGSVVGKLES-PDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGSVVGKLES  +CVPETKLEAKMVEA+R RASKGSIIRSFD IILKFPKID SLRKCKT
Sbjct: 1   MGSVVGKLESHSECVPETKLEAKMVEAIRQRASKGSIIRSFDCIILKFPKIDGSLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IF++FDED NGIIDRRELKECFDGLEISLT+EEIDDLFDACD+STAMG+KFNEFIVLLCL
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180
           VYLLKDDPN    KSQFGMPKLEETFESLVDAF FLD NKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPN---HKSQFGMPKLEETFESLVDAFVFLDTNKDGYVSKSEMVSAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEE 227
           RSSGRI MRRFEEMDWDKNGMVNFKEFLFAFT WVGIDENEDGEEEE
Sbjct: 181 RSSGRIGMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 224

BLAST of Bhi11G000385 vs. ExPASy TrEMBL
Match: A0A0A0L7G7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G061000 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.8e-115
Identity = 214/229 (93.45%), Postives = 220/229 (96.07%), Query Frame = 0

Query: 1   MGSVVGKLESP-DCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGSVVGKLESP +CVPETKLEAKMVE M+ RA+KGSIIRSFD IILKFPKIDDSLR CKT
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELK+CFDGLEI LTEEEIDDLFDACDISTAMGMKFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180
           VYLLKDDPNA FSKSQFGMPKLE+TFESLVDAF FLDKNKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPNAVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEEEE 229
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFT WVGIDENEDGEEEEEE
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEEEE 229

BLAST of Bhi11G000385 vs. ExPASy TrEMBL
Match: A0A1S3BLU5 (probable calcium-binding protein CML21 OS=Cucumis melo OX=3656 GN=LOC103491047 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.8e-110
Identity = 205/224 (91.52%), Postives = 213/224 (95.09%), Query Frame = 0

Query: 1   MGSVVGKLESP-DCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGSVVGKLESP +CVPETKLEAKMVE MR RA+KGSIIRSFD I+LKFPKIDDSLR CKT
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMRRRATKGSIIRSFDCILLKFPKIDDSLRNCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IFQQFDEDLNGIIDRRELK+CFDGLEI LTEEEIDDLF+ACDIS AMG+KFNEFIVLLCL
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISAAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180
           VYLLKDDP+A FSKSQFGMPKLEETFESLVDAF FLDKNKDGYVSKSEMISAINETTSGE
Sbjct: 121 VYLLKDDPDAVFSKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGE 224
           RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFT WVGIDENE+ E
Sbjct: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEEEE 224

BLAST of Bhi11G000385 vs. ExPASy TrEMBL
Match: A0A6J1KV85 (probable calcium-binding protein CML21 OS=Cucurbita maxima OX=3661 GN=LOC111498495 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.0e-108
Identity = 205/227 (90.31%), Postives = 213/227 (93.83%), Query Frame = 0

Query: 1   MGSVVGKLES-PDCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGSVVGKLES  +CVPETKLEAKMVEA+R RASKGSIIRSFD IILKFPKID SLRKCKT
Sbjct: 1   MGSVVGKLESHSECVPETKLEAKMVEAIRQRASKGSIIRSFDCIILKFPKIDGSLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IF++FDED NGIIDRRELKECFDGLEISLT+EEIDDLFDACD+STAMG+KFNEFIVLLCL
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180
           VYLLKDDPN    KSQFGMPKLEETFESLVDAF FLD NKDGYVSKSEM+SAINETTSGE
Sbjct: 121 VYLLKDDPN---HKSQFGMPKLEETFESLVDAFVFLDTNKDGYVSKSEMVSAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEE 227
           RSSGRI MRRFEEMDWDKNGMVNFKEFLFAFT WVGIDENEDGEEEE
Sbjct: 181 RSSGRIGMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 224

BLAST of Bhi11G000385 vs. ExPASy TrEMBL
Match: A0A6J1G727 (probable calcium-binding protein CML21 OS=Cucurbita moschata OX=3662 GN=LOC111451446 PE=4 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 9.8e-108
Identity = 205/227 (90.31%), Postives = 212/227 (93.39%), Query Frame = 0

Query: 1   MGSVVGKLESP-DCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGSVVGKLES  +CVPETKLEAKMVEA+R RASKGSIIRSFD IILKFPKID SLRKCKT
Sbjct: 1   MGSVVGKLESQRECVPETKLEAKMVEAIRQRASKGSIIRSFDCIILKFPKIDASLRKCKT 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
           IF++FDED NGIIDRRELKECFDGLEISLT+EEIDDLFDACD+STAMG+KFNEFIVLLCL
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLKDDPNAKFSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSGE 180
           VYLLKDDPN    KSQFGMPKLEETFESLVDAF FLD NKDGYVSKSEMISAINETTSGE
Sbjct: 121 VYLLKDDPN---HKSQFGMPKLEETFESLVDAFVFLDTNKDGYVSKSEMISAINETTSGE 180

Query: 181 RSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEE 227
           RSSGRI MRRFEEMDWDKNGMVN KEFLFAFT WVGIDENEDGEEEE
Sbjct: 181 RSSGRIGMRRFEEMDWDKNGMVNIKEFLFAFTRWVGIDENEDGEEEE 224

BLAST of Bhi11G000385 vs. ExPASy TrEMBL
Match: A0A6J1DN80 (probable calcium-binding protein CML21 OS=Momordica charantia OX=3673 GN=LOC111022149 PE=4 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.5e-105
Identity = 200/228 (87.72%), Postives = 210/228 (92.11%), Query Frame = 0

Query: 1   MGSVVGKLESP-DCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60
           MGS VGK ESP DCVPETKLEAKMVEAM  RASKGSIIRSFDSIILKFPKIDDS R C +
Sbjct: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60

Query: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120
            FQQFDED NGIIDRRELK+CFDGLEI LTEEEIDDLF+ACDISTAMG+KFNEFIVLL L
Sbjct: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120

Query: 121 VYLLKDDPNAK-FSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSG 180
           VYLL+DDP A  F+KSQFGMPKLEETFESLVDAF FLDKNKDGYVSKSEM+SAI+ETTSG
Sbjct: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAIHETTSG 180

Query: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEE 227
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFT WVG+ ENEDGEEEE
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 228

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G26470.12.6e-8469.30Calcium-binding EF-hand family protein [more]
AT4G26470.25.0e-6465.63Calcium-binding EF-hand family protein [more]
AT3G24110.13.1e-4544.23Calcium-binding EF-hand family protein [more]
AT4G21940.12.1e-0926.92calcium-dependent protein kinase 15 [more]
AT4G21940.22.1e-0926.92calcium-dependent protein kinase 15 [more]
Match NameE-valueIdentityDescription
Q52K823.6e-8369.30Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 ... [more]
Q9LRN64.3e-4444.23Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 ... [more]
O497172.9e-0826.92Calcium-dependent protein kinase 15 OS=Arabidopsis thaliana OX=3702 GN=CPK15 PE=... [more]
Q9ZSA23.8e-0827.56Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=... [more]
Q9M1013.8e-0826.92Calcium-dependent protein kinase 23 OS=Arabidopsis thaliana OX=3702 GN=CPK23 PE=... [more]
Match NameE-valueIdentityDescription
XP_038905190.16.9e-124100.00probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905191.1 proba... [more]
XP_004149571.15.9e-11593.45probable calcium-binding protein CML21 [Cucumis sativus] >KGN56046.1 hypothetica... [more]
XP_008449066.15.7e-11091.52PREDICTED: probable calcium-binding protein CML21 [Cucumis melo][more]
XP_023540519.13.7e-10991.23probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_02354052... [more]
XP_023005531.14.1e-10890.31probable calcium-binding protein CML21 [Cucurbita maxima] >XP_023005532.1 probab... [more]
Match NameE-valueIdentityDescription
A0A0A0L7G72.8e-11593.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G061000 PE=4 SV=1[more]
A0A1S3BLU52.8e-11091.52probable calcium-binding protein CML21 OS=Cucumis melo OX=3656 GN=LOC103491047 P... [more]
A0A6J1KV852.0e-10890.31probable calcium-binding protein CML21 OS=Cucurbita maxima OX=3661 GN=LOC1114984... [more]
A0A6J1G7279.8e-10890.31probable calcium-binding protein CML21 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1DN803.5e-10587.72probable calcium-binding protein CML21 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 147..175
e-value: 0.012
score: 24.8
coord: 185..213
e-value: 3.9
score: 13.0
coord: 56..84
e-value: 2.6
score: 14.4
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 145..210
e-value: 6.1E-8
score: 33.0
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 60..79
e-value: 0.015
score: 14.8
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 143..178
score: 11.417016
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 181..216
score: 9.659605
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 52..87
score: 12.086506
IPR002048EF-hand domainCDDcd00051EFhcoord: 148..211
e-value: 2.48538E-8
score: 47.1573
NoneNo IPR availableGENE3D1.10.238.10coord: 15..213
e-value: 7.7E-29
score: 102.5
NoneNo IPR availablePANTHERPTHR23064TROPONINcoord: 1..223
NoneNo IPR availablePANTHERPTHR23064:SF23CALCIUM-BINDING PROTEIN CML21-RELATEDcoord: 1..223
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 156..168
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 52..215

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M000385Bhi11M000385mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006406 mRNA export from nucleus
molecular_function GO:0005509 calcium ion binding