Chy12G207490.1 (mRNA) Cucumber (hystrix) v1

Overview


Name	Chy12G207490.1
Type	mRNA
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	iron-sulfur assembly protein IscA-like 2, mitochondrial
Location	chrH12: 1693940 .. 1696315 (+)
Sequence length	474
RNA-Seq Expression	Chy12G207490.1
Synteny	Chy12G207490.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCCTAGATCACTAATTCAGAGGGTAACACCATACTTGGTGGCACGTATCAAGGAGAATCAAAGGCTTCTTAGCTCTTCTGCTTCAGCCTATCAAGAAGCATTGTCTTTGTCTCCATCTCCATCTCCATCTGCCCCTGTTGATACCATCTACATGACTGATAGTTGCATTCGGGTATTCTATACATATATCCTTTTTTGCACATACATTTGGCAGATGAATTCTTCATCCATTTCACTGTTGTCTTCTGGTTTAGTTCTTGATTTTCTGGATTATTCTCTTATGGTGCGCCATCCAGGCCTATAGTTTTTGGGTTTCAGGATCCTAATAAATTGTTGTGCGTTTTAGGTTCTTCAAATTCAATTAAATAAATGAATTTTGAGCATTTCTCTTGTTACCTTAGCAGTTCGACCTATATTGGTGTTCATAGAACAGTCACGATTTACATTTTTGCTTAGTTTTGACATGCCTTGCAGAGAATGAAAGAATTGCAAGATCCCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGGAAAAACCAATCCTGATGATAGGTTTGACAAATATTAACTCAGTAATTACTGCTTACTTGGTTTTTCACGTAAAGTTTGTCTCTGATGTTTGATTTTACGTAAAAAAAAGTCTGTTCTCTTTTTTTCATGTTTGCAGGATATATGAGAAAGAGGGAGTTAAATTGGTAGTTGATAACATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCTTTTGTTGTAAGTGAAACTTCTCTCTCATTGTTGCTGCCTGATTGCTTTTGCTTAGATATGTTTATTTCATGCCAATGTGTGGACCATAGCAAATTATAGTTCTATGACATCTATTCAAGTAATGGAGTAATGATAAAGAAAGTTGAAATACTGAAAAACATCCACTAACGCCATCCTTAGAATATCTATCTTTTGACTATTACACCATGCCGTTTATGGAATCTCTCTCCCTTCATAAGTTCTGTTTCGGTATCTTATTTAGAGTATTATTTACGTTGAGAAATATACTGAGATGTAAATCCTCCAGCCCTGAGCTGGGAAACTCTTCACACTGTAGATTCTTAACCTGATATTACACTCGATGACCACTCTCGTCTACCTTCCTCATTTTTACGTATGCACTCGACTTCTAACTGTAAGAGGTGGCGTTCTCCATTTTGCTCTTCCTCGAATATATGTGAACTGTAGGATATAACTTAGGAAAAGTTTTCAACGCCTCGTCAGTATCGATACTTCTCTAATTCTTTGTAACGGTTCCAGTGTCATTTTAGAATGATGTGGGTATAGCAACAATCTAAAATGTTTAGTTCAGAAAATCTATAGTGTGAACAATAAGTAGATGGTGATACTAAAGAATTCTTAAGAATTTCTCCTCGAAGCTACATCTTGGCTAAAAAAACATTTTATTGATAGATGAAATCAAAGGAGAACTCCAAAACACCAAAGAAAAGAGACTAAGAAAGGTATTTACAAAAGAGACTGCCAATTAGTAACTAAGAAAGATAAAAGTATTATGTTTAAAAGGAAAAGAAAGAAAAGAAAAGGCAAAAAAAGAGTGATGTACCCTTGAGCAGTACTATCTACAAGTCGTATTTTATCCAAAAGTTCCCTATGGAATCCTATCTAACCCATCGTAGGATCTGTTTAGATTGACTAGAGAAAAAATGTTTTTTCAAAAAGTCATTTTTATTCTAGGTTGGCGGCAACTGGTGAGAGAGATGGTCGGCATTGGCAACTGGAAGAGGGTTAGGGCAGAGAGGGTTGGTGGTCTCATGGGGAGAATATGAGAAGAGAAGGTGGAGTGCGTGAAGCTTTTCGTTTTAAGAGAAAAAAAAAAAACCTAAAAATTATTGCCATTGGGCCGAACTAATGTGCCGTTACTATGGGTCTTTTTTTCCAGCCATGTTAGGGCAGTGTTCGAAATTTTAAAAAGAAATTGACATGCCAACTGGGGTGTCAGTGTCGGAAACGTGTCTGACACTGATACTGGCCATCTTAGGAGCGTCGGTGCTTCTTAGTTTTTATTTAAACTCTTTTGATAAAAATATTTAAAATAAACTTTAAAAGTGTTTAAAAAGCTATTTTGAGTGGTCGGTAAACTCTTCAATTTTTTCCAAAATAACTTATTTTCAAAATTAAGTGATTGGAAAGTGAAAACGAACACACTTAGGATTAATTGCACTGCAGAAGTTGAATTCTTGTTACGTGCAGGTGAGTACAAATCCAAGTGCTGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAA

mRNA sequence

ATGGGCCCTAGATCACTAATTCAGAGGGTAACACCATACTTGGTGGCACGTATCAAGGAGAATCAAAGGCTTCTTAGCTCTTCTGCTTCAGCCTATCAAGAAGCATTGTCTTTGTCTCCATCTCCATCTCCATCTGCCCCTGTTGATACCATCTACATGACTGATAGTTGCATTCGGAGAATGAAAGAATTGCAAGATCCCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGGAAAAACCAATCCTGATGATAGGATATATGAGAAAGAGGGAGTTAAATTGGTAGTTGATAACATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCTTTTGTTGTGAGTACAAATCCAAGTGCTGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAA

Coding sequence (CDS)

ATGGGCCCTAGATCACTAATTCAGAGGGTAACACCATACTTGGTGGCACGTATCAAGGAGAATCAAAGGCTTCTTAGCTCTTCTGCTTCAGCCTATCAAGAAGCATTGTCTTTGTCTCCATCTCCATCTCCATCTGCCCCTGTTGATACCATCTACATGACTGATAGTTGCATTCGGAGAATGAAAGAATTGCAAGATCCCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGGAAAAACCAATCCTGATGATAGGATATATGAGAAAGAGGGAGTTAAATTGGTAGTTGATAACATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCTTTTGTTGTGAGTACAAATCCAAGTGCTGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAA

Protein sequence

MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSLSPSPSPSAPVDTIYMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ*

Homology

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BLAST of Chy12G207490.1 vs. ExPASy Swiss-Prot
Match: Q8LCY2 (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g03905 PE=1 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 8.4e-55
Identity = 110/155 (70.97%), Postives = 129/155 (83.23%), Query Frame = 0


Query: 4   RSLIQRVTPYLVARIKENQRLLS-SSASAYQEALSLSPSPSPSAPVDTIYMTDSCIRRMK 63
           RSL++RV PYL  RI+EN RLL+ SSASA +EA S S S   S+  D ++++D+CIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQDPK-EEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKG 123
           ELQ  + E+KMLRL VETGGCSGFQY F LD +TNPDDR++EK GVKLVVDN+SYD VKG
Sbjct: 63  ELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVKG 122

Query: 124 ATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 157
           ATIDY EELIR+AFVV+ NPSAVGGCSCKSSFMVK
Sbjct: 123 ATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

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BLAST of Chy12G207490.1 vs. ExPASy Swiss-Prot
Match: Q2TBG7 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 7.9e-29
Identity = 74/147 (50.34%), Postives = 94/147 (63.95%), Query Frame = 0


Query: 10  VTPYLVARIKENQRLLSSSASAYQEALSLSPSPSPSAPVDTIYMTDSCIRRMKELQDPKE 69
           VTP+      +  RLL  +AS    AL      SP A    I++TDSC++R+ E+ +  E
Sbjct: 15  VTPW------QRSRLL--AASRGPRALREVSFSSPEAGEGQIHLTDSCVQRLLEITEGSE 74

Query: 70  EKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKGATIDYVEE 129
              LRL VE GGCSGFQY F+LD   NPDDR++EK G ++VVD+ S  FVKGA +D+ +E
Sbjct: 75  --FLRLEVEGGGCSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVDFSQE 134

Query: 130 LIRSAFVVSTNPSAVGGCSCKSSFMVK 157
           LIRS+F V  NP A  GCSC SSF VK
Sbjct: 135 LIRSSFQVLNNPQAQQGCSCGSSFSVK 151

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BLAST of Chy12G207490.1 vs. ExPASy Swiss-Prot
Match: Q86U28 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 3.9e-28
Identity = 66/129 (51.16%), Postives = 87/129 (67.44%), Query Frame = 0


Query: 28  SASAYQEALSLSPSPSPSAPVDTIYMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQY 87
           +AS   +A   + S SP A    I +TDSC++R+ E+ +  E   LRL VE GGCSGFQY
Sbjct: 27  TASLGPQARREASSSSPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQY 86

Query: 88  VFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGC 147
            F+LD   NPDDR++E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP A  GC
Sbjct: 87  KFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGC 146

Query: 148 SCKSSFMVK 157
           SC SSF +K
Sbjct: 147 SCGSSFSIK 153

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BLAST of Chy12G207490.1 vs. ExPASy Swiss-Prot
Match: Q5R788 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.9e-28
Identity = 66/129 (51.16%), Postives = 87/129 (67.44%), Query Frame = 0


Query: 28  SASAYQEALSLSPSPSPSAPVDTIYMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQY 87
           +AS   +A   + S SP A    I +TDSC++R+ E+ +  E   LRL VE GGCSGFQY
Sbjct: 27  AASLGPQARREASSSSPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQY 86

Query: 88  VFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGC 147
            F+LD   NPDDR++E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP A  GC
Sbjct: 87  KFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGC 146

Query: 148 SCKSSFMVK 157
           SC SSF +K
Sbjct: 147 SCGSSFSIK 153

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BLAST of Chy12G207490.1 vs. ExPASy Swiss-Prot
Match: Q9DCB8 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 GN=Isca2 PE=1 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 2.0e-27
Identity = 63/116 (54.31%), Postives = 80/116 (68.97%), Query Frame = 0


Query: 41  SPSPSAPVDTIYMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDR 100
           S  P A    I +TDSC++R+ E+ +  E   LRL VE GGCSGFQY F+LD   NPDDR
Sbjct: 40  SSIPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQYKFSLDTVINPDDR 99

Query: 101 IYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 157
           ++E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP A  GCSC SSF VK
Sbjct: 100 VFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSVK 153

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BLAST of Chy12G207490.1 vs. ExPASy TrEMBL
Match: A0A0A0LXX8 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G496290 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.5e-80
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSLSPSPSPSAPVDTIYMTDSCIRR 60
           MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEAL LSPSPSPSAPVDTI+MTDSCIRR
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60

Query: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120
           MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157

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BLAST of Chy12G207490.1 vs. ExPASy TrEMBL
Match: A0A1S3C5P3 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103497193 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.6e-77
Identity = 153/159 (96.23%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEA--LSLSPSPSPSAPVDTIYMTDSCI 60
           MGPRSLIQRVTPYLVARIKENQRLLSSSA+AYQEA  LS SPSPSPSAPVDTI+MTDSCI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSPSAPVDTIHMTDSCI 60

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKT PDDRIYEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

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BLAST of Chy12G207490.1 vs. ExPASy TrEMBL
Match: A0A6J1HPD1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464825 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.6e-70
Identity = 139/159 (87.42%), Postives = 148/159 (93.08%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSL--SPSPSPSAPVDTIYMTDSCI 60
           M PR LIQR+TP+LVARI+ENQRLLSSS SA+QEA SL  SPSPS S P+DTI MTD+CI
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           RRMKELQ PKEEKMLRLSVETGGCSGFQYVF+LD KTNPDDR+YEKEGV+LVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G207490.1 vs. ExPASy TrEMBL
Match: A0A6J1I3Y1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111469443 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.8e-69
Identity = 138/159 (86.79%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSL--SPSPSPSAPVDTIYMTDSCI 60
           M  R LIQR+TP+LVARI+ENQRLLSSS SA+QEA SL  SPSPS S P+DTI MTD+CI
Sbjct: 1   MASRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           RRMKELQ PKEEKMLRLSVETGGCSGFQYVF+LD KTNPDDR+YEKEGV+LVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G207490.1 vs. ExPASy TrEMBL
Match: A0A6J1DIX6 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111020448 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.9e-66
Identity = 131/159 (82.39%), Postives = 145/159 (91.19%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSL--SPSPSPSAPVDTIYMTDSCI 60
           M PR LIQR+TP+ +ARI+ENQRLLSSS SA+QE  SL  SPSPS S  +DTI+MTD+CI
Sbjct: 1   MVPRLLIQRLTPHFIARIRENQRLLSSSVSAHQEVPSLLTSPSPSSSVLIDTIHMTDNCI 60

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           ++MKELQ+P EEKMLRLSVETGGCSGFQYVFNLD KTNPDDR+YEK+GVKLVVDNISYDF
Sbjct: 61  QKMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDKTNPDDRVYEKDGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           VKGATIDY EELIRSAFVV+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYAEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 159

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BLAST of Chy12G207490.1 vs. NCBI nr
Match: XP_004152897.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis sativus] >KGN65682.1 hypothetical protein Csa_019688 [Cucumis sativus])

HSP 1 Score: 306 bits (784), Expect = 8.28e-105
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSLSPSPSPSAPVDTIYMTDSCIRR 60
           MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEAL LSPSPSPSAPVDTI+MTDSCIRR
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60

Query: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120
           MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157
           GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157

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BLAST of Chy12G207490.1 vs. NCBI nr
Match: XP_008457520.2 (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis melo] >XP_008457521.1 PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis melo] >XP_008457522.1 PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis melo])

HSP 1 Score: 297 bits (761), Expect = 2.85e-101
Identity = 153/159 (96.23%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSLS--PSPSPSAPVDTIYMTDSCI 60
           MGPRSLIQRVTPYLVARIKENQRLLSSSA+AYQEA SLS  PSPSPSAPVDTI+MTDSCI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSPSAPVDTIHMTDSCI 60

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKT PDDRIYEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G207490.1 vs. NCBI nr
Match: XP_038893859.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_038893860.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_038893861.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 273 bits (698), Expect = 1.07e-91
Identity = 137/157 (87.26%), Postives = 146/157 (92.99%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSLSPSPSPSAPVDTIYMTDSCIRR 60
           M  RSLIQRVTPYLVARI+ENQRLLSSS SA+QEA S    PSPS  VDTI++TD+CIRR
Sbjct: 1   MASRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSSLTCPSPSPSVDTIHLTDNCIRR 60

Query: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120
           MKELQDPKEEKMLRLSVETGGCSGFQYVF+LD KTNPDDR+YEKEGVKL+VDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLIVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157
           GATIDYVEELIRSAF+VSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 GATIDYVEELIRSAFIVSTNPSAVGGCSCKSSFMVKQ 157

[Close]

BLAST of Chy12G207490.1 vs. NCBI nr
Match: XP_022964844.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata] >XP_023520276.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 272 bits (696), Expect = 2.32e-91
Identity = 139/159 (87.42%), Postives = 148/159 (93.08%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSL--SPSPSPSAPVDTIYMTDSCI 60
           M PR LIQR+TP+LVARI+ENQRLLSSS SA+QEA SL  SPSPS S P+DTI MTD+CI
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           RRMKELQ PKEEKMLRLSVETGGCSGFQYVF+LD KTNPDDR+YEKEGV+LVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G207490.1 vs. NCBI nr
Match: XP_022970473.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 269 bits (688), Expect = 3.84e-90
Identity = 138/159 (86.79%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALSL--SPSPSPSAPVDTIYMTDSCI 60
           M  R LIQR+TP+LVARI+ENQRLLSSS SA+QEA SL  SPSPS S P+DTI MTD+CI
Sbjct: 1   MASRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           RRMKELQ PKEEKMLRLSVETGGCSGFQYVF+LD KTNPDDR+YEKEGV+LVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Chy12G207490.1 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 214.5 bits (545), Expect = 6.0e-56
Identity = 110/155 (70.97%), Postives = 129/155 (83.23%), Query Frame = 0


Query: 4   RSLIQRVTPYLVARIKENQRLLS-SSASAYQEALSLSPSPSPSAPVDTIYMTDSCIRRMK 63
           RSL++RV PYL  RI+EN RLL+ SSASA +EA S S S   S+  D ++++D+CIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQDPK-EEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKG 123
           ELQ  + E+KMLRL VETGGCSGFQY F LD +TNPDDR++EK GVKLVVDN+SYD VKG
Sbjct: 63  ELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVKG 122

Query: 124 ATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 157
           ATIDY EELIR+AFVV+ NPSAVGGCSCKSSFMVK
Sbjct: 123 ATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

[Close]

BLAST of Chy12G207490.1 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 72.0 bits (175), Expect = 4.8e-13
Identity = 38/117 (32.48%), Postives = 64/117 (54.70%), Query Frame = 0


Query: 44  PSAPVDTIYMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPD----- 103
           P+     + +TD    R+  L   +++  LRL V+  GC+G  Y  N  GKT+ +     
Sbjct: 16  PALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYAGKTSGNDEKGK 75

Query: 104 -DRIYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM 155
            D + E++GV+++V+  +   V G  +D+V++ +RS FV   NP++ G C C  SFM
Sbjct: 76  FDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPNSQGQCGCGESFM 131

[Close]

BLAST of Chy12G207490.1 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-12
Identity = 35/111 (31.53%), Postives = 60/111 (54.05%), Query Frame = 0


Query: 44  PSAPVDTIYMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYE 103
           P+     + +TD    R+  L   +++  LRL V+  GC+G  Y  N   +    D + E
Sbjct: 16  PALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVE 75

Query: 104 KEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM 155
           ++GV+++V+  +   V G  +D+V++ +RS FV   NP++ G C C  SFM
Sbjct: 76  EKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPNSQGQCGCGESFM 125

[Close]

BLAST of Chy12G207490.1 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 69.3 bits (168), Expect = 3.1e-12
Identity = 36/101 (35.64%), Postives = 60/101 (59.41%), Query Frame = 0


Query: 53  MTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVD 112
           ++D+   R+++L   +++  LRL+VE  GC+G  YV N   +    D + E++GVK++VD
Sbjct: 8   LSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVD 67

Query: 113 NISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSF 154
             +   V G  +D+V++ +RS FV   NP+A   C C  SF
Sbjct: 68  PKAVMHVIGTEMDFVDDKLRSEFVF-VNPNAT-KCGCGESF 106

[Close]

BLAST of Chy12G207490.1 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.0e-07
Identity = 24/89 (26.97%), Postives = 47/89 (52.81%), Query Frame = 0


Query: 44  PSAPVDTIYMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYE 103
           P+     + +TD    R+  L   +++  LRL V+  GC+G  Y  N   +    D + E
Sbjct: 16  PALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVE 75

Query: 104 KEGVKLVVDNISYDFVKGATIDYVEELIR 133
           ++GV+++V+  +   V G  +D+V++ +R
Sbjct: 76  EKGVRILVEPKALMHVIGTKMDFVDDKLR 104

The following BLAST results are available for this feature:

BLAST of Chy12G207490.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LCY2	8.4e-55	70.97	Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q2TBG7	7.9e-29	50.34	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=I...	[more]
Q86U28	3.9e-28	51.16	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN...	[more]
Q5R788	3.9e-28	51.16	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN...	[more]
Q9DCB8	2.0e-27	54.31	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 G...	[more]

BLAST of Chy12G207490.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXX8	3.5e-80	98.73	Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G496290...	[more]
A0A1S3C5P3	1.6e-77	96.23	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucumis melo OX=3656 ...	[more]
A0A6J1HPD1	5.6e-70	87.42	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX...	[more]
A0A6J1I3Y1	4.8e-69	86.79	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3...	[more]
A0A6J1DIX6	2.9e-66	82.39	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia O...	[more]

BLAST of Chy12G207490.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152897.1	8.28e-105	98.73	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis sativus] >KGN65...	[more]
XP_008457520.2	2.85e-101	96.23	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis melo...	[more]
XP_038893859.1	1.07e-91	87.26	iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_...	[more]
XP_022964844.1	2.32e-91	87.42	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata] >XP...	[more]
XP_022970473.1	3.84e-90	86.79	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima]	[more]

BLAST of Chy12G207490.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03905.1	6.0e-56	70.97	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.3	4.8e-13	32.48	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.1	1.4e-12	31.53	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	3.1e-12	35.64	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.2	3.0e-07	26.97	Iron-sulphur cluster biosynthesis family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 51..155 e-value: 4.3E-33 score: 111.7
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 51..150 e-value: 7.3E-15 score: 55.1
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 50..157 e-value: 1.6E-33 score: 117.1
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 50..143
None	No IPR available	PANTHER	PTHR43011:SF4	BNACNNG10620D PROTEIN	coord: 4..157
None	No IPR available	PANTHER	PTHR43011	IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL	coord: 4..157

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Chy12G207490	Chy12G207490	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G207490.1.exon1	Chy12G207490.1.exon1	exon
Chy12G207490.1.exon2	Chy12G207490.1.exon2	exon
Chy12G207490.1.exon3	Chy12G207490.1.exon3	exon
Chy12G207490.1.exon4	Chy12G207490.1.exon4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G207490.1.cds1	Chy12G207490.1.cds1	CDS
Chy12G207490.1.cds2	Chy12G207490.1.cds2	CDS
Chy12G207490.1.cds3	Chy12G207490.1.cds3	CDS
Chy12G207490.1.cds4	Chy12G207490.1.cds4	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Chy12G207490.1	Chy12G207490.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0106035	protein maturation by [4Fe-4S] cluster transfer
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding