Chy10G172710.1 (mRNA) Cucumber (hystrix) v1

Overview


Name	Chy10G172710.1
Type	mRNA
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Cysteine proteinase inhibitor 4
Location	chrH10: 1948939 .. 1949231 (-)
Sequence length	293
RNA-Seq Expression	Chy10G172710.1
Synteny	Chy10G172710.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTGTGTACAAGTACCTCCAAATACTCATGACCTAATTGAGGATGTCCACAGCCCAGACGTTGTAGAAGCTGGAAAGATAGCAGTGGAAGAACATAACAAGAAAGAAAATGACAATTTAGTTTTCATTCGTGTAGTGAGTGGTTTAGCACCCCGTGCTCTTATTTTGATTAAATTATGGTCATATATTCTTGTTATAGAAGCTAAAACTTGCAATGGATATCCTGTGACATATGTGGTTGAAATAGATAAGTATCTTACAATTCCAAACAATAGCTATAAACTTGTCAG

mRNA sequence

ATGAGTTGTGTACAAGTACCTCCAAATACTCATGACCTAATTGAGGATGTCCACAGCCCAGACGTTGTAGAAGCTGGAAAGATAGCAGTGGAAGAACATAACAAGAAAGAAAATGACAATTTAGTTTTCATTCGTGTAGTGAGTGGTTTAGCACCCCGTGCTCTTATTTTGATTAAATTATGGTCATATATTCTTGTTATAGAAGCTAAAACTTGCAATGGATATCCTGTGACATATGTGGTTGAAATAGATAAGTATCTTACAATTCCAAACAATAGCTATAAACTTGTCAG

Coding sequence (CDS)

ATGAGTTGTGTACAAGTACCTCCAAATACTCATGACCTAATTGAGGATGTCCACAGCCCAGACGTTGTAGAAGCTGGAAAGATAGCAGTGGAAGAACATAACAAGAAAGAAAATGACAATTTAGTTTTCATTCGTGTAGTGAGTGGTTTAGCACCCCGTGCTCTTATTTTGATTAAATTATGGTCATATATTCTTGTTATAGAAGCTAAAACTTGCAATGGATATCCTGTGACATATGTGGTTGAAATAGATAAGTATCTTACAATTCCAAACAATAGCTATAAACTTGTCAG

Protein sequence

MSCVQVPPNTHDLIEDVHSPDVVEAGKIAVEEHNKKENDNLVFIRVVSGLAPRALILIKLWSYILVIEAKTCNGYPVTYVVEIDKYLTIPNNSYKLVX

Homology

[Close]

BLAST of Chy10G172710.1 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 44.7 bits (104), Expect = 7.1e-04
Identity = 28/80 (35.00%), Postives = 41/80 (51.25%), Query Frame = 0


Query: 14  IEDVHSPDVVEAGKIAVEEHNKKENDNLVFIRVVSGLAPRALILIKLWSYILVIEAKTCN 73
           I++V  PDVV   K A+EEHNK+  + LVF++VV G       ++    Y L I AK   
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQ----VVSGTKYDLKIAAKDGG 94

Query: 74  GYPVTYVVEIDKYLTIPNNS 94
           G    Y   + + L + + S
Sbjct: 95  GKIKNYEAVVVEKLWLHSKS 110

[Close]

BLAST of Chy10G172710.1 vs. NCBI nr
Match: KAE8648094.1 (hypothetical protein Csa_004738 [Cucumis sativus])

HSP 1 Score: 181 bits (459), Expect = 4.78e-57
Identity = 87/97 (89.69%), Postives = 93/97 (95.88%), Query Frame = 0


Query: 1  MSCVQVPPNTHDLIEDVHSPDVVEAGKIAVEEHNKKENDNLVFIRVVSGLAPRALILIKL 60
          MSCVQVPPNTHDLI+DVHSPDVVEAGK+AVEEHNKKENDNLVFIRVVS +APRALI IKL
Sbjct: 1  MSCVQVPPNTHDLIKDVHSPDVVEAGKVAVEEHNKKENDNLVFIRVVSAIAPRALIFIKL 60

Query: 61 WSYILVIEAKTCNGYPVTYVVEIDKYLTIPNNSYKLV 97
          WS+ILVIEAKTCNG+PVTYVVEIDKYLTIP  SYKL+
Sbjct: 61 WSFILVIEAKTCNGFPVTYVVEIDKYLTIPEESYKLI 97

[Close]

BLAST of Chy10G172710.1 vs. NCBI nr
Match: KAE8648095.1 (hypothetical protein Csa_004720 [Cucumis sativus])

HSP 1 Score: 160 bits (404), Expect = 1.17e-48
Identity = 79/97 (81.44%), Postives = 87/97 (89.69%), Query Frame = 0


Query: 1  MSCVQVPPNTHDLIEDVHSPDVVEAGKIAVEEHNKKENDNLVFIRVVSGLAPRALILIKL 60
          MSCVQV PNTHDLI+DVHSP+V+EAGKIAV+EHNKKENDNLVF RVVSGLAPRA+I   L
Sbjct: 1  MSCVQVLPNTHDLIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTIL 60

Query: 61 WSYILVIEAKTCNGYPVTYVVEIDKYLTIPNNSYKLV 97
          WSYILVIEAK C+GYPV YV +I KYLTIP NSY+LV
Sbjct: 61 WSYILVIEAKNCDGYPVAYVAKIVKYLTIPENSYELV 97

[Close]

BLAST of Chy10G172710.1 vs. NCBI nr
Match: KAF8093897.1 (hypothetical protein N665_0375s0014 [Sinapis alba])

HSP 1 Score: 49.3 bits (116), Expect = 6.40e-05
Identity = 25/62 (40.32%), Postives = 37/62 (59.68%), Query Frame = 0


Query: 13 LIEDVHSPDVVEAGKIAVEEHNKKENDNLVFIRVVSGLAPRALILIKLWSYILVIEAKTC 72
          LI+DV +P+V E  K A++EHN + N+NLVF+ ++ G       L+    Y L+I AK  
Sbjct: 30 LIKDVSAPNVKEVAKYAIDEHNSESNENLVFVEIIEGKRQ----LLSAIIYDLIIAAKNN 87

Query: 73 NG 74
           G
Sbjct: 90 GG 87

[Close]

BLAST of Chy10G172710.1 vs. NCBI nr
Match: VFQ68095.1 (unnamed protein product [Cuscuta campestris])

HSP 1 Score: 49.3 bits (116), Expect = 7.06e-05
Identity = 29/59 (49.15%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 16 DVHSPDVVEAGKIAVEEHNKKENDNLVFIRVVSGLAPRALILIKLWSYILVIEAKTCNG 74
          D  SP+VV AG  AVE HN+ ENDNLVF  V SG    A      + Y LVI+A++ +G
Sbjct: 36 DPKSPEVVAAGSFAVEAHNEAENDNLVFEDVASGEKQMA----ARFQYKLVIDARSGHG 90

[Close]

BLAST of Chy10G172710.1 vs. NCBI nr
Match: XP_018469246.1 (PREDICTED: cysteine proteinase inhibitor 4-like [Raphanus sativus])

HSP 1 Score: 47.8 bits (112), Expect = 2.52e-04
Identity = 18/36 (50.00%), Postives = 28/36 (77.78%), Query Frame = 0


Query: 14 IEDVHSPDVVEAGKIAVEEHNKKENDNLVFIRVVSG 49
          I+DV +P+V+E  K A++EHN + NDNL+F+ +V G
Sbjct: 31 IKDVSAPNVIEVAKYAIDEHNSETNDNLMFVEIVEG 66

[Close]

BLAST of Chy10G172710.1 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 5.1e-05
Identity = 28/80 (35.00%), Postives = 41/80 (51.25%), Query Frame = 0


Query: 14  IEDVHSPDVVEAGKIAVEEHNKKENDNLVFIRVVSGLAPRALILIKLWSYILVIEAKTCN 73
           I++V  PDVV   K A+EEHNK+  + LVF++VV G       ++    Y L I AK   
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQ----VVSGTKYDLKIAAKDGG 94

Query: 74  GYPVTYVVEIDKYLTIPNNS 94
           G    Y   + + L + + S
Sbjct: 95  GKIKNYEAVVVEKLWLHSKS 110

The following BLAST results are available for this feature:

BLAST of Chy10G172710.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WT8	7.1e-04	35.00	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]

BLAST of Chy10G172710.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Chy10G172710.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648094.1	4.78e-57	89.69	hypothetical protein Csa_004738 [Cucumis sativus]	[more]
KAE8648095.1	1.17e-48	81.44	hypothetical protein Csa_004720 [Cucumis sativus]	[more]
KAF8093897.1	6.40e-05	40.32	hypothetical protein N665_0375s0014 [Sinapis alba]	[more]
VFQ68095.1	7.06e-05	49.15	unnamed protein product [Cuscuta campestris]	[more]
XP_018469246.1	2.52e-04	50.00	PREDICTED: cysteine proteinase inhibitor 4-like [Raphanus sativus]	[more]

BLAST of Chy10G172710.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16500.1	5.1e-05	35.00	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 14..79 e-value: 1.1E-9 score: 38.4
None	No IPR available	GENE3D	3.10.450.10		coord: 9..96 e-value: 3.3E-10 score: 41.8
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 19..78

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Chy10G172710	Chy10G172710	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy10G172710.1.exon1	Chy10G172710.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy10G172710.1.cds1	Chy10G172710.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Chy10G172710.1	Chy10G172710.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0005773	vacuole
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity