Chy5G099970 (gene) Cucumber (hystrix) v1

BLAST of Chy5G099970 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 2.2e-227
Identity = 390/544 (71.69%), Postives = 461/544 (84.74%), Query Frame = 0

Query: 1   MDHDEQRNGQ-DFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAE 60
           M++++Q + Q +FIF+SKLPDIYIPNHLPLH+YCFE++SQF+ RPCLING TG I TYA+
Sbjct: 1   MENNKQDDHQEEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYAD 60

Query: 61  VHLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIV 120
           V L +R+ AAGL K+GI QGDVIML+LQNSPEFV+AFL AS+ GAI TTANP Y P E+ 
Sbjct: 61  VDLTSRKVAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVA 120

Query: 121 KQAVAAKAKVIITQGEFVEKVWEF-AVEHGVKILCTDSSSASESAGCLKFSEVMEADENE 180
           KQA A+K K++IT   +++KV EF   E GVK++C D+        CL FSE+ +ADE E
Sbjct: 121 KQAAASKTKLVITLAGYIDKVKEFTGGESGVKVMCVDAPPPESE--CLHFSELTQADETE 180

Query: 181 IPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICV 240
           IPAVKI+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDG+NPN+  ++ DVI+CV
Sbjct: 181 IPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCV 240

Query: 241 LPLFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTP 300
           LPLFHIYSLNS+LLCGLRVGAAILI+QK++IS LL LI+  K TIAPFVPPIVL  AK P
Sbjct: 241 LPLFHIYSLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCP 300

Query: 301 DIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEA 360
           D+HRYDLSSIR VMSG APMGK+LED VK KLP+A LGQGYGMTEAGPVLSMCL FAKE 
Sbjct: 301 DLHRYDLSSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEP 360

Query: 361 FKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDK 420
           F +KSGACGTVVRNAEMKI+ P T  SLPRN  GEICIRG QIMKGY+N+ EAT+ TID+
Sbjct: 361 FPIKSGACGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDE 420

Query: 421 DGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMED 480
            GWLHTGD+GFID+DDE+FIVDRLKELIKYKG+QVAPAELE++L S+PNIT AAV+PM+D
Sbjct: 421 GGWLHTGDIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKD 480

Query: 481 EAAGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKIL 540
           EAAGE+PVAFVVRS GSKI+E DIK YIS QV++YK+I K FF++ IP  PSGKILRKIL
Sbjct: 481 EAAGEVPVAFVVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKIL 540

Query: 541 KAQL 543
           +A+L
Sbjct: 541 RAKL 542

BLAST of Chy5G099970 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 2.1e-222
Identity = 374/542 (69.00%), Postives = 458/542 (84.50%), Query Frame = 0

Query: 5   EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVHLAA 64
           E +   D IF+SKLPDIYIP HLPLH+YCFE++S+F  RPCLING   +I+TYAEV L  
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 65  RRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVA 124
           R+ A GL+K+GI Q D IM++L NSPEFVFAF+GAS+ GAI+T ANPL+ P E+VKQA A
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 125 AKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKI 184
           + AK+IITQ  FV KV ++A E+ VK++C DS+      GCL FSE+ ++DE+EIP VKI
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSA----PEGCLHFSELTQSDEHEIPEVKI 187

Query: 185 NSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHI 244
             +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+ +DV++CVLPLFHI
Sbjct: 188 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 247

Query: 245 YSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDIHRYD 304
           YSLNS+LLCGLRVGAAILI+QK+DI+  L LIQ +K +I PFVPPIVL  AK+P +  YD
Sbjct: 248 YSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYD 307

Query: 305 LSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSG 364
           LSS+R VMSGAAP+GK+LED V+ K PNA LGQGYGMTEAGPVL+MCL FAKE F +KSG
Sbjct: 308 LSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 367

Query: 365 ACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHT 424
           ACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TIDK+GWLHT
Sbjct: 368 ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHT 427

Query: 425 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEI 484
           GD+GFID+DDE+FIVDRLKELIKYKG+QVAPAE+EALL ++PNI+ AAV+PM+DE AGE+
Sbjct: 428 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEV 487

Query: 485 PVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEA 544
           PVAFVVRS GS I+E+++K++IS QVI+YK++++VFFV+++P +PSGKILRK L+A+L A
Sbjct: 488 PVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAA 545

Query: 545 GV 547
           GV
Sbjct: 548 GV 545

BLAST of Chy5G099970 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 3.6e-222
Identity = 375/546 (68.68%), Postives = 462/546 (84.62%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MD + +++G D IF+SKLPDIYIP HLPLH+YCFE+LS+F  RPCLI+G   RI+TYAEV
Sbjct: 3   MDIETKQSG-DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 62

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +R+ A GL+K+GI Q D IM++L N PEFVFAF+GAS+ GAI+T ANPL+ P E+VK
Sbjct: 63  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 122

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+ AK++ITQ  F  KV ++A+E+ +K++C DS+      GC+ FSE++++DE+EIP
Sbjct: 123 QAKASSAKIVITQACFAGKVKDYAIENDLKVICVDSA----PEGCVHFSELIQSDEHEIP 182

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
            VKI  +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+  DV++CVLP
Sbjct: 183 DVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLP 242

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLC LRVGAAILI+QK+DI+  L LI  HK TI PFVPPIVL  AK+P +
Sbjct: 243 LFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLV 302

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H YDLSS+R VMSGAAP+GK+LED V+AK PNA LGQGYGMTEAGPVL+MCL FAKE F 
Sbjct: 303 HNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 362

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TI+K+G
Sbjct: 363 IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEG 422

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE 
Sbjct: 423 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQ 482

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFVVRS GS I+E+++K++IS QVI+YK+I++VFFV+++P +PSGKILRK L+A
Sbjct: 483 AGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 542

Query: 541 QLEAGV 547
           +L AG+
Sbjct: 543 RLAAGI 543

BLAST of Chy5G099970 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 8.9e-221
Identity = 374/546 (68.50%), Postives = 460/546 (84.25%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MD + +++G D IF+SKLPDIYIP HLPLH+YCFE+LS+F  RPCLI+G   RI+TYAEV
Sbjct: 3   MDTETKQSG-DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 62

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +R+ A GL+K+GI Q D IM++L N PEFVFAF+GAS+ GAI+T ANPL+ P E+VK
Sbjct: 63  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 122

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+ AK++ITQ  F  KV ++A+E+ +K++C D    S   GC+ FSE++++DE+EIP
Sbjct: 123 QAKASSAKIVITQACFAGKVKDYAIENDLKVICVD----SVPEGCVHFSELIQSDEHEIP 182

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
            VKI  +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+  DV++CVLP
Sbjct: 183 DVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLP 242

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLC LRVGAAILI+QK+DI+  L LI  HK TI PFVPPIVL  AK+P +
Sbjct: 243 LFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLV 302

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
             YDLSS+R VMSGAAP+GK+LED V+AK PNA LGQGYGMTEAGPVL+MCL FAKE F 
Sbjct: 303 DNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 362

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TI+K+G
Sbjct: 363 IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEG 422

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE 
Sbjct: 423 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQ 482

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFVVRS GS I+E+++K++IS QVI+YK+I++VFFV+++P +PSGKILRK L+A
Sbjct: 483 AGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 542

Query: 541 QLEAGV 547
           +L AG+
Sbjct: 543 RLAAGI 543

BLAST of Chy5G099970 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 2.0e-220
Identity = 371/546 (67.95%), Postives = 458/546 (83.88%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           M  + + N  D IF+SKLPDIYIP HLPLH+YCFE++S+F  RPCLING    I+TYA+V
Sbjct: 1   MPMETETNQGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +R+ AAGL+K+GI Q D IM++L NSPEFVFAF+GAS+ GAI+T ANPL+ P E+VK
Sbjct: 61  ELTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+ AK+IITQ  FV KV ++A ++ + ++C DS+      GC+ FSE+ +ADE++IP
Sbjct: 121 QAKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSA----PEGCIHFSELTQADEHDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
            VKI S+DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+ +DV++CVLP
Sbjct: 181 DVKIQSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILI+QK+DI     LI+ +K TI PFVPPIVL  AK+P +
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVV 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
             YDLSS+R VMSGAAP+GK+LED V+ K PNA LGQGYGMTEAGPVL+MCL FAKE F 
Sbjct: 301 DNYDLSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+  AT +TIDK+G
Sbjct: 361 IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+G+ID+DDE+FIVDRLKELIKYKG+QVAPAELEALL ++PNI+ AAV+PM+DE 
Sbjct: 421 WLHTGDIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQ 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFVVRS GS I+E+++K+++S QVI+YK+I++VFFV+++P +PSGKILRK L+A
Sbjct: 481 AGEVPVAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 540

Query: 541 QLEAGV 547
           +L AGV
Sbjct: 541 RLAAGV 542

BLAST of Chy5G099970 vs. ExPASy TrEMBL
Match: A0A0A0LM95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G048330 PE=4 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 8.2e-310
Identity = 539/547 (98.54%), Postives = 545/547 (99.63%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDHDEQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAAK KVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP
Sbjct: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINS+DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP
Sbjct: 181 AVKINSSDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQTHKATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540

Query: 541 QLEAGVF 548
           QLEAGVF
Sbjct: 541 QLEAGVF 547

BLAST of Chy5G099970 vs. ExPASy TrEMBL
Match: A0A1S3BSW8 (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103493360 PE=4 SV=1)

HSP 1 Score: 1043.1 bits (2696), Expect = 4.1e-301
Identity = 526/547 (96.16%), Postives = 539/547 (98.54%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+AKVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIRGPQIMKGYLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 540

Query: 541 QLEAGVF 548
           QLEAGVF
Sbjct: 541 QLEAGVF 546

BLAST of Chy5G099970 vs. ExPASy TrEMBL
Match: A0A5A7U0C7 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1468G00210 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.7e-294
Identity = 519/547 (94.88%), Postives = 532/547 (97.26%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+AKVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIR       YLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIR-------YLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 539

Query: 541 QLEAGVF 548
           QLEAGVF
Sbjct: 541 QLEAGVF 539

BLAST of Chy5G099970 vs. ExPASy TrEMBL
Match: A0A5D3CKT4 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00190 PE=4 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.1e-290
Identity = 514/547 (93.97%), Postives = 527/547 (96.34%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+AKVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+P            GYLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHP------------GYLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 534

Query: 541 QLEAGVF 548
           QLEAGVF
Sbjct: 541 QLEAGVF 534

BLAST of Chy5G099970 vs. ExPASy TrEMBL
Match: A0A6J1EP35 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436262 PE=4 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.2e-274
Identity = 475/540 (87.96%), Postives = 505/540 (93.52%), Query Frame = 0

Query: 8   NGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVHLAARRF 67
           N QDFIF+SKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN  TG+IHTYA V LAARR 
Sbjct: 7   NAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRV 66

Query: 68  AAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKA 127
           A GLSKIG+GQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQA  A+ 
Sbjct: 67  ATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARP 126

Query: 128 KVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKINSN 187
           KVIITQ EFVEKV EFAVE+GVKI+CTD    S   GCL+FSE++EADENEIPAVKINSN
Sbjct: 127 KVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPAVKINSN 186

Query: 188 DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSL 247
           DVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++N  DV+IC+LPLFHIYSL
Sbjct: 187 DVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSL 246

Query: 248 NSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDIHRYDLSS 307
           NSVLLCGLRVGAAILILQKYD+SSLLRLIQTHKATIAPFVPPIVLDFAK PDIHRYDLSS
Sbjct: 247 NSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 306

Query: 308 IRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACG 367
           IRIVMSGAAPMGK LEDTVKA+LPNA LGQGYGMTEAGPVLSMCLGFAKE F+ KSGACG
Sbjct: 307 IRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACG 366

Query: 368 TVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDL 427
           TVVRNAEMKIIHP+T +SLPRN+PGEICIRG QIMKGYLNNKEATEKTIDK+GWLHTGDL
Sbjct: 367 TVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTGDL 426

Query: 428 GFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVA 487
           GFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAAGEIP A
Sbjct: 427 GFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAA 486

Query: 488 FVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEAGVF 547
           FVVRSKGSKISE+DIK YISDQVIYYKKIRKVFFVDSIP+APSGKILRK+LKAQLE G F
Sbjct: 487 FVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLENGAF 542

BLAST of Chy5G099970 vs. NCBI nr
Match: XP_004149075.1 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KGN61091.1 hypothetical protein Csa_021253 [Cucumis sativus])

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 539/547 (98.54%), Postives = 545/547 (99.63%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDHDEQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAAK KVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP
Sbjct: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINS+DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP
Sbjct: 181 AVKINSSDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQTHKATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 547

BLAST of Chy5G099970 vs. NCBI nr
Match: XP_008452293.1 (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1043 bits (2698), Expect = 0.0
Identity = 526/547 (96.16%), Postives = 539/547 (98.54%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+AKVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIRGPQIMKGYLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 540

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 546

BLAST of Chy5G099970 vs. NCBI nr
Match: KAA0048648.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1021 bits (2641), Expect = 0.0
Identity = 519/547 (94.88%), Postives = 532/547 (97.26%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+AKVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIR       YLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIR-------YLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 539

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 539

BLAST of Chy5G099970 vs. NCBI nr
Match: TYK10849.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 514/547 (93.97%), Postives = 527/547 (96.34%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+AKVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PG            YLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPG------------YLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 534

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 534

BLAST of Chy5G099970 vs. NCBI nr
Match: XP_038890953.1 (4-coumarate--CoA ligase 2-like [Benincasa hispida])

HSP 1 Score: 969 bits (2506), Expect = 0.0
Identity = 488/547 (89.21%), Postives = 514/547 (93.97%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH    NGQDFIF+SKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLING TG +HTYA V
Sbjct: 1   MDHGLM-NGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGATGEVHTYAAV 60

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            LAARR AAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAI+TTANPLYKPGEI K
Sbjct: 61  DLAARRVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAK 120

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA  A+ KVIITQ EFVEKVW +AVEHGVKILCTDS       GCL+FSEVMEADEN+IP
Sbjct: 121 QAAGARPKVIITQAEFVEKVWGYAVEHGVKILCTDSPPV----GCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVN+   DVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLYSNDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 300
           LFHIYSLNSVLLCGLRVGAAILIL+KYD+SSLL LIQT+KATIAPFVPPIVLDFAK PDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILKKYDMSSLLPLIQTYKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           HRYDLSSIRIVMSGAAPMGKDLEDTVKA+LPNA LGQGYGMTEAGPVLSMCL FAKE F+
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFE 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKII+P+TS+SLPRN+PGEICIRG QIMKGY+NN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIINPETSVSLPRNHPGEICIRGSQIMKGYINNREATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGFIDD+DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNI+SAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDNDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNISSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISE+D+K YISDQVIYYKKIRKVFFVDSIP+APSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISEDDLKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKA 540

Query: 541 QLEAGVF 547
           QLEAG F
Sbjct: 541 QLEAGAF 542

BLAST of Chy5G099970 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 717.2 bits (1850), Expect = 9.8e-207
Identity = 353/550 (64.18%), Postives = 437/550 (79.45%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           M+     N  D IF+SKLPDIYIPNHL LH Y F+++S+F  +PCLING TG ++TY++V
Sbjct: 12  MEKQSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDV 71

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           H+ +R+ AA   K+G+ Q DV+ML+L N PEFV +FL ASF GA AT ANP + P EI K
Sbjct: 72  HVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAK 131

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILC-TDSSSASESAGCLKFSEVMEAD---E 180
           QA A+  K+IIT+  +V+K+     + GV I+C  D+ S     GCL+F+E+ ++     
Sbjct: 132 QAKASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEAS 191

Query: 181 NEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVII 240
             I +V+I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +  DVI+
Sbjct: 192 EVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVIL 251

Query: 241 CVLPLFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAK 300
           CVLP+FHIY+LNS++LCGLRVGAAILI+ K++I+ LL LIQ  K T+AP VPPIVL  AK
Sbjct: 252 CVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAK 311

Query: 301 TPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAK 360
           + +  +YDLSSIR+V SGAAP+GK+LED V AK PNA LGQGYGMTEAGPVL+M LGFAK
Sbjct: 312 SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAK 371

Query: 361 EAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTI 420
           E F VKSGACGTVVRNAEMKI+ P T  SL RN PGEICIRG QIMKGYLNN  AT +TI
Sbjct: 372 EPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETI 431

Query: 421 DKDGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPM 480
           DKDGWLHTGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+IT  AV+ M
Sbjct: 432 DKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAM 491

Query: 481 EDEAAGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRK 540
           ++EAAGE+PVAFVV+SK S++SE+D+K ++S QV++YK+I KVFF +SIP APSGKILRK
Sbjct: 492 KEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRK 551

Query: 541 ILKAQLEAGV 547
            L+A+L  G+
Sbjct: 552 DLRAKLANGL 561

BLAST of Chy5G099970 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 716.5 bits (1848), Expect = 1.7e-206
Identity = 355/546 (65.02%), Postives = 438/546 (80.22%), Query Frame = 0

Query: 3   HDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVHL 62
           +D+++   D IF+S+LPDIYIPNHLPLH Y FE++S+F  +PCLING TG ++TYA+VH+
Sbjct: 12  NDQKQCSNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHV 71

Query: 63  AARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQA 122
            +R+ AAGL  +G+ Q DV+M++L NSPE V  FL ASF GAI T+ANP + P EI KQA
Sbjct: 72  TSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQA 131

Query: 123 VAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAV 182
            A+ AK+I+TQ  +V+K+       GV I+ TDS +  E+  CL+FSE+ +++E  + ++
Sbjct: 132 KASAAKLIVTQSRYVDKIKNLQ-NDGVLIVTTDSDAIPEN--CLRFSELTQSEEPRVDSI 191

Query: 183 --KINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 242
             KI+  DVVALPFSSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  NR DVI+CVLP
Sbjct: 192 PEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLP 251

Query: 243 LFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDI 302
           +FHIY+LNS++LC LRVGA ILI+ K++I+ LL  IQ  K T+A  VPPIVL  AK+P+ 
Sbjct: 252 MFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPET 311

Query: 303 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 362
            +YDLSS+R+V SGAAP+GK+LED + AK PNA LGQGYGMTEAGPVL+M LGFAKE F 
Sbjct: 312 EKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFP 371

Query: 363 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 422
           VKSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+  AT  TIDKDG
Sbjct: 372 VKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDG 431

Query: 423 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 482
           WLHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELE+LL  +P I   AV+ M++E 
Sbjct: 432 WLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEED 491

Query: 483 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 542
           AGE+PVAFVVRSK S ISE++IK ++S QV++YK+I KVFF DSIP APSGKILRK L+A
Sbjct: 492 AGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRA 551

Query: 543 QLEAGV 547
           +L  G+
Sbjct: 552 RLANGL 554

BLAST of Chy5G099970 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 669.5 bits (1726), Expect = 2.4e-192
Identity = 330/514 (64.20%), Postives = 407/514 (79.18%), Query Frame = 0

Query: 1   MDHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           M+     N  D IF+SKLPDIYIPNHL LH Y F+++S+F  +PCLING TG ++TY++V
Sbjct: 12  MEKQSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDV 71

Query: 61  HLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           H+ +R+ AA   K+G+ Q DV+ML+L N PEFV +FL ASF GA AT ANP + P EI K
Sbjct: 72  HVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAK 131

Query: 121 QAVAAKAKVIITQGEFVEKVWEFAVEHGVKILC-TDSSSASESAGCLKFSEVMEAD---E 180
           QA A+  K+IIT+  +V+K+     + GV I+C  D+ S     GCL+F+E+ ++     
Sbjct: 132 QAKASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEAS 191

Query: 181 NEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVII 240
             I +V+I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +  DVI+
Sbjct: 192 EVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVIL 251

Query: 241 CVLPLFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAK 300
           CVLP+FHIY+LNS++LCGLRVGAAILI+ K++I+ LL LIQ  K T+AP VPPIVL  AK
Sbjct: 252 CVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAK 311

Query: 301 TPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAK 360
           + +  +YDLSSIR+V SGAAP+GK+LED V AK PNA LGQGYGMTEAGPVL+M LGFAK
Sbjct: 312 SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAK 371

Query: 361 EAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTI 420
           E F VKSGACGTVVRNAEMKI+ P T  SL RN PGEICIRG QIMKGYLNN  AT +TI
Sbjct: 372 EPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETI 431

Query: 421 DKDGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPM 480
           DKDGWLHTGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+IT  AV+ M
Sbjct: 432 DKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAM 491

Query: 481 EDEAAGEIPVAFVVRSKGSKISENDIKNYISDQV 511
           ++EAAGE+PVAFVV+SK S++SE+D+K ++S QV
Sbjct: 492 KEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Chy5G099970 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 664.5 bits (1713), Expect = 7.6e-191
Identity = 327/532 (61.47%), Postives = 415/532 (78.01%), Query Frame = 0

Query: 13  IFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVHLAARRFAAGLS 72
           IF+SKLPDI IPNHLPLHTYCFE LS    +PCLI G+TG+ +TY E HL  RR A+GL 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 73  KIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKAKVIIT 132
           K+GI +GDVIM++LQNS EFVF+F+GAS  GA++TTANP Y   E+ KQ  ++ AK+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 133 QGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENE--IPAVKINSNDVV 192
             ++V+K+        + ++ TD  +      CL FS ++  DE       V I  +D  
Sbjct: 154 HSQYVDKLKNLG--ENLTLITTDEPTPE---NCLPFSTLITDDETNPFQETVDIGGDDAA 213

Query: 193 ALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSLNSV 252
           ALPFSSGTTG+PKGV+LTHKSL+TSVAQQVDG+NPN+ +   DVI+CVLPLFHIYSLNSV
Sbjct: 214 ALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSV 273

Query: 253 LLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVLDFAKTPDIHRYDLSSIRI 312
           LL  LR GA +L++ K++I +LL LIQ H+ TIA  VPP+V+  AK P ++ YDLSS+R 
Sbjct: 274 LLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF 333

Query: 313 VMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACGTVV 372
           V+SGAAP+GK+L+D+++ +LP AILGQGYGMTEAGPVLSM LGFAKE    KSG+CGTVV
Sbjct: 334 VLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVV 393

Query: 373 RNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDLGFI 432
           RNAE+K++H +T LSL  N PGEICIRG QIMK YLN+ EAT  TID++GWLHTGD+G++
Sbjct: 394 RNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYV 453

Query: 433 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVV 492
           D+DDEIFIVDRLKE+IK+KG+QV PAELE+LL ++ +I  AAV+P  DE AGE+PVAFVV
Sbjct: 454 DEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVV 513

Query: 493 RSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQL 543
           RS G+ I+E D+K Y++ QV++YK++ KVFFV SIP +PSGKILRK LKA+L
Sbjct: 514 RSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Chy5G099970 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 649.0 bits (1673), Expect = 3.3e-186
Identity = 317/551 (57.53%), Postives = 420/551 (76.23%), Query Frame = 0

Query: 2   DHDEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLS----QFQHRPCLINGTTGRIHTY 61
           + +E+    DFIF+SKLPDI+IPNHLPL  Y F+  S          C+I+G TGRI TY
Sbjct: 17  EDEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTY 76

Query: 62  AEVHLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGE 121
           A+V    RR AAG+ ++GI  GDV+ML+L NSPEF  +FL  ++ GA++TTANP Y   E
Sbjct: 77  ADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPE 136

Query: 122 IVKQAVAAKAKVIITQGEFVEKVWEFAVEHGVKILCTDSSS-----ASESAGCLKFSEVM 181
           I KQA A+ AK+IIT+   V+K+       GV I+C D        +S   GC+ F+E+ 
Sbjct: 137 IAKQAKASAAKMIITKKCLVDKLTNLK-NDGVLIVCLDDDGDNGVVSSSDDGCVSFTELT 196

Query: 182 EADENEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRK 241
           +ADE E+   KI+  D VA+P+SSGTTG+PKGVM+THK LVTS+AQ+VDGENPN+N    
Sbjct: 197 QADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTAN 256

Query: 242 DVIICVLPLFHIYSLNSVLLCGLRVGAAILILQKYDISSLLRLIQTHKATIAPFVPPIVL 301
           DVI+C LP+FHIY+L++++L  +R GAA+LI+ +++++ ++ LIQ +K T+ P  PP+VL
Sbjct: 257 DVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVL 316

Query: 302 DFAKTPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCL 361
            F K+P+  RYDLSS+RI++SGAA + K+LED V+ K PNAI GQGYGMTE+G V +  L
Sbjct: 317 AFIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSL 376

Query: 362 GFAKEAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEAT 421
            FAK  FK KSGACGTV+RNAEMK++  +T +SLPRN  GEIC+RG Q+MKGYLN+ EAT
Sbjct: 377 AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEAT 436

Query: 422 EKTIDKDGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAA 481
            +TIDKDGWLHTGD+GF+DDDDEIFIVDRLKELIK+KGYQVAPAELEALL S+P+I  AA
Sbjct: 437 ARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAA 496

Query: 482 VIPMEDEAAGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGK 541
           V+ M+DE A E+PVAFV RS+GS+++E+D+K+Y++ QV++YK+I+ VFF++ IP A SGK
Sbjct: 497 VVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGK 556

Query: 542 ILRKILKAQLE 544
           ILRK L+A+LE
Sbjct: 557 ILRKDLRAKLE 565