Chy5G097190 (gene) Cucumber (hystrix) v1

Overview
NameChy5G097190
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionsphinganine C4-monooxygenase 1-like
LocationchrH05: 8554856 .. 8557107 (+)
RNA-Seq ExpressionChy5G097190
SyntenyChy5G097190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGGTTACCTTCTTTCTTTTCTTTTCTTTTACCTATTTTTTCAATTCTTAATCCTTGTTCTTCCCGGTTTGGTTAATTTTGTTGTTATTAAGCTCATTTCTGATTCTAATTGTTTGTGAAACTTGCCCTAAAAAATTTCAACTGAGATTGTATTTTGCTCTAGACTTTGCCGAAACTCTTTATTTGATCTTCCTGAATTTTTCTTGGTTTATCAGCAGCTCTTTGTGGTACAGCTTGATTATAGTTTTACTAGACTAGATACAAATTCGTTGTGGGTTTTGTTGTTTCTGGACCTATTCAGTTTGACAAAATCTAAATAATTACTTCTCTTCTGTTTACATGTGTTTTAAATTTTGATTTAGACAAGAACAGACTAGACACTGGATGGGGGGAGTGAAGAAGCAATGTCATTGTTGACCTATTCTCAACTTTCGGTTTCTTATAATTACAACTATTGTATATCTTAAACAAGATGTGTTGCTTACTTTTGTTACTTTAGTTCGAATTGATGGAGCAAAATTCTAAACCTTGTCACATCAAAATAATTTAGATGGCAATTGAAATTTTTTAATTGGTTCAAGAATAAGAGGAGTTAATGATTCTAAGTTAAGGAAGTTGAAAGACAAAATCTGGTTATTAGCTTTTGACTGTAAGTATGTTTTTGTTTCGAACACTACAAGGAAGGCTTTTGGTGAATACTTTAACTTCAAGATAGACATGAACAATTTTTTTTGCATATTACCCCTTTTTCGTAGCTAAATATTCTCTATGAGATGGTTATTTGGACTAGAAAGTAATGGGCTAAAACATGATATTTTTGTTTTCTTTGTAAATTAAATTTCATTTCCTTTAAGTATATTAACCTTTTGATTTGTCTATTCATTTTAGGTTACATGTTTATTCACAGTTTTAGGACCAATCCCCATTTGTGTTCTGCATCGGCACTCGGTTGGAAGTCACCAACTTGCAAACAAAAAAATCTTGAACTTCCGTTTAATTTTGTGGTATTTTGCTTGTATTTCCTGTAATGTTGTTCCCATTGCTTCTAGTCCATCGAGTTTCTTTTCTTTTTGAGTCCCATGCTAAACTAGTTTAGCAGTTGTTCGTGTGAAATATTGTAGACTGAACTAAAACTATTGTTATTATTAACCTCATCCAAGTGCTGTTAGATGAATTGTTCGGTTTGTCGTCCCTGTGGTTAAAGTGGCAAGTCCTGAACTCAAATGTTTTGAGCATACGAGCTCAAACTAAGATGCCACCTATTTGGGGCATCTTAGAAATCCTGGCTTTGTAATATTCTAAAGTTTAGTAGTTGTCTTGTGATATTATTCAAAATGTATGTAAACTAACTCAGAGACTTCAGTTTTTGAAAAAGAGAAAAGAAAAGGAATCATTGCGAACTTTGGCATAAGTCATTGATATTATGTGGGGTGTCTCTGCACTTACGTTCGAGTTTGGTCTCTGAATTTATAATTGCATTTTGTTATGTTCAGGTGACTGGAAATGATGATGGAAGCAATGCAGTTCTAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCGTTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGGTTGGTGGGGCGTTGTCATTTCTAGCATCTGGTATGACTCCTAGAGTCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAGGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAA

mRNA sequence

ATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGGTGACTGGAAATGATGATGGAAGCAATGCAGTTCTAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCGTTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGGTTGGTGGGGCGTTGTCATTTCTAGCATCTGGTATGACTCCTAGAGTCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAGGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAA

Coding sequence (CDS)

ATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGGTGACTGGAAATGATGATGGAAGCAATGCAGTTCTAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCGTTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGGTTGGTGGGGCGTTGTCATTTCTAGCATCTGGTATGACTCCTAGAGTCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAGGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAA

Protein sequence

MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKTVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEKRAGGGFEARPKIE*
Homology
BLAST of Chy5G097190 vs. ExPASy Swiss-Prot
Match: Q8VYI1 (Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 7.0e-112
Identity = 189/254 (74.41%), Postives = 220/254 (86.61%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGT  PI VYW YSG+Y+ L   ++YRLHSK +E+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFIQAIVAIILFKVTGND-DGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKF 120
           QQ +QA+VAI+LF VTG+D +   A   S+ ++  QF+TAM VLDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIK 180
           LY+HIHSQHHRL+VPYA+GALYNHPVEGLLLDT+GGALSFL SGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG+K+NFSQPFFV WDRILGTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

BLAST of Chy5G097190 vs. ExPASy Swiss-Prot
Match: Q9AST3 (Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 2.7e-111
Identity = 188/251 (74.90%), Postives = 215/251 (85.66%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F ISD+ LGTFVPI VYW YSG+Y+ LG  D YRLHSK DEDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFIQAIVAIILFKVTGND-DGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKF 120
           QQ +QAI+++ILFK+TG+D D +     S  ++  QF+ AM V+DTWQYF HRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIK 180
           LY+HIHSQHHRL+VPY++GALYNHP+EGLLLDT+GGALSFL SGM+PR +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGN FHI F NN+AYHDVHHQLYG+K+NFSQPFFV WDRILGTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRAGGGFEARP 251
           KRA GGFE RP
Sbjct: 242 KRANGGFETRP 252

BLAST of Chy5G097190 vs. ExPASy Swiss-Prot
Match: B8B4W4 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39946 GN=GL1-9 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.4e-96
Identity = 169/251 (67.33%), Postives = 203/251 (80.88%), Query Frame = 0

Query: 5   ISDDLLGTFVPIFVYWFYSGLYLLLGF---FDNYRLHSKTDEDEKNLVSKSTVVRGVLFQ 64
           +SD+ +GTF PI +YW Y+G Y L+      + YRLH++ +E+EKNLV+   VVRGVL Q
Sbjct: 8   VSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGVLLQ 67

Query: 65  QFIQAIVAIILFKVTGNDDGSNAVLKSWPMVV--LQFLTAMFVLDTWQYFAHRYMHHNKF 124
           Q +QAIVA+ILF VT +   S+AV+   P+++   QFL AM V+D+WQYF HRYMH NKF
Sbjct: 68  QLVQAIVAMILFMVTSD---SSAVVVQPPIIIQAFQFLVAMLVMDSWQYFVHRYMHQNKF 127

Query: 125 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIK 184
           LYRHIHSQHHRL+VPYA GALYNHP+EGLLLDTVGGA+SFL SGMTPR S+FFF FA +K
Sbjct: 128 LYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVLK 187

Query: 185 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 244
           TVDDHCGLWLP N+F  LF+NNTAYHDVHHQL GSK+N+SQPFF  WDRILGT+MPY+L 
Sbjct: 188 TVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNLV 247

Query: 245 KRAGGGFEARP 251
           +R  GGFEARP
Sbjct: 248 RRKEGGFEARP 255

BLAST of Chy5G097190 vs. ExPASy Swiss-Prot
Match: Q0D4G3 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-9 PE=2 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.4e-96
Identity = 169/251 (67.33%), Postives = 203/251 (80.88%), Query Frame = 0

Query: 5   ISDDLLGTFVPIFVYWFYSGLYLLLGF---FDNYRLHSKTDEDEKNLVSKSTVVRGVLFQ 64
           +SD+ +GTF PI +YW Y+G Y L+      + YRLH++ +E+EKNLV+   VVRGVL Q
Sbjct: 8   VSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGVLLQ 67

Query: 65  QFIQAIVAIILFKVTGNDDGSNAVLKSWPMVV--LQFLTAMFVLDTWQYFAHRYMHHNKF 124
           Q +QAIVA+ILF VT +   S+AV+   P+++   QFL AM V+D+WQYF HRYMH NKF
Sbjct: 68  QLVQAIVAMILFMVTSD---SSAVVVQPPIIIQAFQFLVAMLVMDSWQYFVHRYMHQNKF 127

Query: 125 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIK 184
           LYRHIHSQHHRL+VPYA GALYNHP+EGLLLDTVGGA+SFL SGMTPR S+FFF FA +K
Sbjct: 128 LYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVLK 187

Query: 185 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 244
           TVDDHCGLWLP N+F  LF+NNTAYHDVHHQL GSK+N+SQPFF  WDRILGT+MPY+L 
Sbjct: 188 TVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNLV 247

Query: 245 KRAGGGFEARP 251
           +R  GGFEARP
Sbjct: 248 RRKEGGFEARP 255

BLAST of Chy5G097190 vs. ExPASy Swiss-Prot
Match: O94298 (Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sur2 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.0e-54
Identity = 108/253 (42.69%), Postives = 148/253 (58.50%), Query Frame = 0

Query: 15  PIFVYWFYSGL-----YLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQAIVA 74
           P+ +YW  S       Y+ L  F+ YR+H   +   +N V +  VV+ VLFQQ  + +V 
Sbjct: 21  PVIIYWVASAFFGFLHYIELPVFEKYRIHPPEEIARRNRVPQMAVVKAVLFQQLCEVVVG 80

Query: 75  IILFKVTGNDDGSN-----------------AVLKSWPMV----------VLQFLTAMFV 134
           I L    G  +  +                 A+L+  P              Q+  A F+
Sbjct: 81  IALAMFEGYPEPIDEAKQMLRYEAFFSKNLPALLQVAPFAPKLAYNFIVPAFQYFFAFFI 140

Query: 135 LDTWQYFAHRYMHHNKFLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLAS 194
           +D+WQYF HRY+H+NK LY  IH+ HHRL VPYA GALYNHP EGL+LDT G  +++LA+
Sbjct: 141 IDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDTFGAGVAYLAA 200

Query: 195 GMTPRVSIFFFSFATIKTVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPF 236
           G++P+ ++ FF+ +T+KTVDDHCG   P +   + F NN  YHD+HHQ YG + NFSQPF
Sbjct: 201 GLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPYGFQKNFSQPF 260

BLAST of Chy5G097190 vs. ExPASy TrEMBL
Match: A0A5D3CZU2 (Sphinganine C4-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1327G00180 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.9e-144
Identity = 251/253 (99.21%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGSNAV KSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. ExPASy TrEMBL
Match: A0A1S3B426 (sphinganine C4-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103485785 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.9e-144
Identity = 251/253 (99.21%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGSNAV KSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. ExPASy TrEMBL
Match: A0A6J1DY75 (sphinganine C4-monooxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111025684 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 5.4e-139
Identity = 239/253 (94.47%), Postives = 249/253 (98.42%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISD+LLGTFVPIFVYW YSG+Y+LLGFF+NYRLHSK DEDEKNLVSK+TVVRGVLF
Sbjct: 1   MEFTISDELLGTFVPIFVYWLYSGIYVLLGFFENYRLHSKKDEDEKNLVSKATVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS+AV KSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSSAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           YR+IHSQHHRLVVPYAFGALYNHPVEGLLLDT+GGALSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 YRYIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSK+NFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. ExPASy TrEMBL
Match: A0A6J1GBQ9 (sphinganine C4-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452712 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.6e-138
Identity = 239/253 (94.47%), Postives = 247/253 (97.63%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y+LLGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS AV KSWP+VVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSVAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDTVGGALSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. ExPASy TrEMBL
Match: A0A6J1KH66 (sphinganine C4-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493180 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 7.8e-138
Identity = 238/253 (94.07%), Postives = 247/253 (97.63%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y++LGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS AV KSWP+VVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDTVGGALSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. NCBI nr
Match: XP_008441706.1 (PREDICTED: sphinganine C4-monooxygenase 1-like [Cucumis melo] >KAA0032268.1 sphinganine C4-monooxygenase 1-like [Cucumis melo var. makuwa] >TYK17072.1 sphinganine C4-monooxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 519 bits (1337), Expect = 6.11e-186
Identity = 251/253 (99.21%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGSNAV KSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 253
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. NCBI nr
Match: XP_004138918.1 (sphinganine C4-monooxygenase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 515 bits (1326), Expect = 2.91e-184
Identity = 247/253 (97.63%), Postives = 251/253 (99.21%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGSNAV KSWPMVVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDT+GGALSFLASGMTPR+SIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 253
           RA GGFEARPKIE
Sbjct: 241 RASGGFEARPKIE 253

BLAST of Chy5G097190 vs. NCBI nr
Match: XP_038889456.1 (sphinganine C4-monooxygenase 1-like [Benincasa hispida])

HSP 1 Score: 509 bits (1311), Expect = 5.63e-182
Identity = 245/253 (96.84%), Postives = 250/253 (98.81%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSG+Y+LLGFF+NYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQ IQAIVAIILFKVTGNDDGSNAV KSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQIIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           Y+HIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YKHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHI+F NNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHIIFGNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 253
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. NCBI nr
Match: XP_022159270.1 (sphinganine C4-monooxygenase 1-like [Momordica charantia])

HSP 1 Score: 501 bits (1290), Expect = 8.96e-179
Identity = 239/253 (94.47%), Postives = 249/253 (98.42%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISD+LLGTFVPIFVYW YSG+Y+LLGFF+NYRLHSK DEDEKNLVSK+TVVRGVLF
Sbjct: 1   MEFTISDELLGTFVPIFVYWLYSGIYVLLGFFENYRLHSKKDEDEKNLVSKATVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS+AV KSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSSAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           YR+IHSQHHRLVVPYAFGALYNHPVEGLLLDT+GGALSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 YRYIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSK+NFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 253
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. NCBI nr
Match: XP_022949326.1 (sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >KAG6607119.1 Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036810.1 Sphinganine C4-monooxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 498 bits (1282), Expect = 1.49e-177
Identity = 239/253 (94.47%), Postives = 247/253 (97.63%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y+LLGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKVTGNDDGS AV KSWP+VVLQFLTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSVAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDTVGGALSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RAGGGFEARPKIE 253
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

BLAST of Chy5G097190 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 404.8 bits (1039), Expect = 5.0e-113
Identity = 189/254 (74.41%), Postives = 220/254 (86.61%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGT  PI VYW YSG+Y+ L   ++YRLHSK +E+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFIQAIVAIILFKVTGND-DGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKF 120
           QQ +QA+VAI+LF VTG+D +   A   S+ ++  QF+TAM VLDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIK 180
           LY+HIHSQHHRL+VPYA+GALYNHPVEGLLLDT+GGALSFL SGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG+K+NFSQPFFV WDRILGTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

BLAST of Chy5G097190 vs. TAIR 10
Match: AT1G14290.1 (sphingoid base hydroxylase 2 )

HSP 1 Score: 402.9 bits (1034), Expect = 1.9e-112
Identity = 188/251 (74.90%), Postives = 215/251 (85.66%), Query Frame = 0

Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F ISD+ LGTFVPI VYW YSG+Y+ LG  D YRLHSK DEDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFIQAIVAIILFKVTGND-DGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKF 120
           QQ +QAI+++ILFK+TG+D D +     S  ++  QF+ AM V+DTWQYF HRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIK 180
           LY+HIHSQHHRL+VPY++GALYNHP+EGLLLDT+GGALSFL SGM+PR +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGN FHI F NN+AYHDVHHQLYG+K+NFSQPFFV WDRILGTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRAGGGFEARP 251
           KRA GGFE RP
Sbjct: 242 KRANGGFETRP 252

BLAST of Chy5G097190 vs. TAIR 10
Match: AT2G29390.3 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 79.7 bits (195), Expect = 3.7e-15
Identity = 71/240 (29.58%), Postives = 109/240 (45.42%), Query Frame = 0

Query: 10  LGTFVPIFVYWFYSGL-YLLL---GFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SGL Y+ L   GF  NY++ +K++  E        + R +L+   + 
Sbjct: 17  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 76

Query: 70  ---AIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G +  S+  L SW +V  Q L    + D   Y+ HR + H K+LY+
Sbjct: 77  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 136

Query: 130 HIHSQHHRLVVPYAFGALYNHPVEGLLL---DTVGGALSFLASGMTPRVSIFFFSFATIK 189
           ++HS HH    P+   + Y HP E L L     VG AL    +G        +     I+
Sbjct: 137 NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIE 196

Query: 190 TVDDHCGL---WLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPY 237
           TV+ HCG    W P N   +       +HD HH+L  +K       FV  D I GT   Y
Sbjct: 197 TVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGY 244

BLAST of Chy5G097190 vs. TAIR 10
Match: AT2G29390.2 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 79.7 bits (195), Expect = 3.7e-15
Identity = 71/240 (29.58%), Postives = 109/240 (45.42%), Query Frame = 0

Query: 10  LGTFVPIFVYWFYSGL-YLLL---GFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SGL Y+ L   GF  NY++ +K++  E        + R +L+   + 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  ---AIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G +  S+  L SW +V  Q L    + D   Y+ HR + H K+LY+
Sbjct: 84  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 143

Query: 130 HIHSQHHRLVVPYAFGALYNHPVEGLLL---DTVGGALSFLASGMTPRVSIFFFSFATIK 189
           ++HS HH    P+   + Y HP E L L     VG AL    +G        +     I+
Sbjct: 144 NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIE 203

Query: 190 TVDDHCGL---WLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPY 237
           TV+ HCG    W P N   +       +HD HH+L  +K       FV  D I GT   Y
Sbjct: 204 TVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGY 251

BLAST of Chy5G097190 vs. TAIR 10
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-14
Identity = 72/250 (28.80%), Postives = 111/250 (44.40%), Query Frame = 0

Query: 10  LGTFVPIFVYWFYSGL-YLLL---GFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SGL Y+ L   GF  NY++ +K++  E        + R +L+   + 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  ---AIVAIILFKVTGNDDGSNAVLKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G +  S+  L SW +V  Q L    + D   Y+ HR + H K+LY+
Sbjct: 84  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 143

Query: 130 HIHSQHHRLVVPYAFGALYNHPVEGLLL---DTVGGALSFLASGMTPRVSIFFFSFATIK 189
           ++HS HH    P+   + Y HP E L L     VG AL    +G        +     I+
Sbjct: 144 NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWMMLRVIE 203

Query: 190 TVDDHCGL---WLPGNLFHIL----------FRNNTAYHDVHHQLYGSKFNFSQPFFVTW 237
           TV+ HCG    W P N   +           F  +  +HD HH+L  +K       FV  
Sbjct: 204 TVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYSSTFVYM 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYI17.0e-11274.41Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1[more]
Q9AST32.7e-11174.90Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1[more]
B8B4W45.4e-9667.33Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39... [more]
Q0D4G35.4e-9667.33Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=... [more]
O942981.0e-5442.69Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Match NameE-valueIdentityDescription
A0A5D3CZU21.9e-14499.21Sphinganine C4-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3B4261.9e-14499.21sphinganine C4-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103485785 PE=3... [more]
A0A6J1DY755.4e-13994.47sphinganine C4-monooxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC1110256... [more]
A0A6J1GBQ94.6e-13894.47sphinganine C4-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11145271... [more]
A0A6J1KH667.8e-13894.07sphinganine C4-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493180 ... [more]
Match NameE-valueIdentityDescription
XP_008441706.16.11e-18699.21PREDICTED: sphinganine C4-monooxygenase 1-like [Cucumis melo] >KAA0032268.1 sphi... [more]
XP_004138918.12.91e-18497.63sphinganine C4-monooxygenase 1 isoform X1 [Cucumis sativus][more]
XP_038889456.15.63e-18296.84sphinganine C4-monooxygenase 1-like [Benincasa hispida][more]
XP_022159270.18.96e-17994.47sphinganine C4-monooxygenase 1-like [Momordica charantia][more]
XP_022949326.11.49e-17794.47sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >KAG6607119.1 Sphingani... [more]
Match NameE-valueIdentityDescription
AT1G69640.15.0e-11374.41sphingoid base hydroxylase 1 [more]
AT1G14290.11.9e-11274.90sphingoid base hydroxylase 2 [more]
AT2G29390.33.7e-1529.58sterol 4-alpha-methyl-oxidase 2-2 [more]
AT2G29390.23.7e-1529.58sterol 4-alpha-methyl-oxidase 2-2 [more]
AT2G29390.11.1e-1428.80sterol 4-alpha-methyl-oxidase 2-2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 97..232
e-value: 2.4E-23
score: 82.9
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 2..249
NoneNo IPR availablePANTHERPTHR11863:SF122SPHINGANINE C4-MONOOXYGENASE 1-LIKEcoord: 2..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G097190.1Chy5G097190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016491 oxidoreductase activity