Chy4G087530 (gene) Cucumber (hystrix) v1

Overview
NameChy4G087530
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMajor facilitator superfamily protein
LocationchrH04: 25964268 .. 25965980 (+)
RNA-Seq ExpressionChy4G087530
SyntenyChy4G087530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACCTCCGGCAACCCCTTCTGGCATCAGTTGCTCTTTGGGCGGTGGTTCTCTGCCTTTGCCTCCATTCTTATGATGTCCGTCTCCGGGGCCGCCTTCATGTTTGCCCTCTACTCCTCCGACATCAAATATTCCTTTGGCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGCCTTAGGCGGCAATGCCGGCATCATCTGCGGCCTCATCAATGAGGTAGCTCCGACTTGGATGATTCTTCTAATCGGCGCCGTCATGAACCTTTTTGGCTACATCATGATTTGGCTAGCCGTAACCAAACGGATTCCCAAGCCCCAAATATGGCATATGTGTCTCTACATTTGCATCGGAGCTAATTCTCAGACCTTTACAAATACTGGGGCCATTGTAACCTTCGTCAATAACTTCCCGGAAAGTCGTGGTTGTGTTTTGGGACTTTTGAAGGGTTACGTCGGCTTAAGCGGCGCCATTTTGTCCCAGCTTTACCATGCCTTTTATGGCAACAATTCCAAATCTTTTATTCTCTTAATTGCTTGGCTTCCCGCCGCCATCACTGTTGTTTGCCTCCGTTTCGTTCGCATCATTAAGGATCTCCGGCTGCCCAACGAGGTCAAAGTTTTATACCATATCCTTTATATTTCTCTCGGCCTTGCTGGGTTGTTAATGGTTTTTATTATTTTGCAAAATCGTATCGGGTTTCATCAAATACAATACATCGGAAGTGCCATTGTTGTGATTGTTCTGCTTTTACTGCCTCTGGCCATTGTTTTCAGAGAGGAATTGATTGTTTGGAAGAGTAAAATCGGAAACCCAAATGCACAACTAGAGTTAGCATCTCAACAACCGCCGCCGCCACTTATCTCGGCAGTGCCACTGAGCCCTCCGTCGGACTCCTGCTTTAAAAACATGTTTAAACCCCCAAACAGAGGAGAGGACTACACAATCCCTCAAGCCATTTTCAGCGTCGATATGATCATTCTTTTTATCGCCTTAATCTGTGGTGTCGGTGGAACGTTGGCGGCGATGGACAATTTGGGTCAAATCAGCGAGTCTTTGGAGTACCCATCTCAGAGTACCACCACATTCATCTCTCTGGTCAGCATATGGAATTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTATTTCTCTTTGGGATGCTTATTTTATCCTGCGTCGGCTATCTTCTAATCGCATTCGGAGTTCCACATTCACTGTATGTTTCTTCAATCATAATTGGGTTCTGCTTTGGAGCGCAGTTGCCATTGATCTTGGCAATCATATCGGAAATTTTTGGGTTGAAGTACTATGCGACTCTAAGCAATTTGGGAGTAGCTGCAAACCCTATTGGGACGTATATTATGAATGTGAAAGTGGTGGGTCATTTGTACGACAGGGAGGCTGAACGGCAGATGGAGGCAGCAGGGGGACGAAGAAAAATAGGGGAGAATTTGAGTTGCTTGGGAGTGGAGTGTTACAGAAAGGCATTTCTTATAATCACGGGGACGACGGTGTTGGGAGGAGTTGTTTCCCTAATTTTAGTGGTTCGGACATGGAAGTTTTACAAGGGCGATATTTATAAGAAGTTCAGAGAGGAGGAAGGTGAAGACGTCGAGATCAAAACCACCGCATCGACCAAGACGCCTAAAACTGAATCTGTTTAG

mRNA sequence

ATGGCAACCTCCGGCAACCCCTTCTGGCATCAGTTGCTCTTTGGGCGGTGGTTCTCTGCCTTTGCCTCCATTCTTATGATGTCCGTCTCCGGGGCCGCCTTCATGTTTGCCCTCTACTCCTCCGACATCAAATATTCCTTTGGCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGCCTTAGGCGGCAATGCCGGCATCATCTGCGGCCTCATCAATGAGGTAGCTCCGACTTGGATGATTCTTCTAATCGGCGCCGTCATGAACCTTTTTGGCTACATCATGATTTGGCTAGCCGTAACCAAACGGATTCCCAAGCCCCAAATATGGCATATGTGTCTCTACATTTGCATCGGAGCTAATTCTCAGACCTTTACAAATACTGGGGCCATTGTAACCTTCGTCAATAACTTCCCGGAAAGTCGTGGTTGTGTTTTGGGACTTTTGAAGGGTTACGTCGGCTTAAGCGGCGCCATTTTGTCCCAGCTTTACCATGCCTTTTATGGCAACAATTCCAAATCTTTTATTCTCTTAATTGCTTGGCTTCCCGCCGCCATCACTGTTGTTTGCCTCCGTTTCGTTCGCATCATTAAGGATCTCCGGCTGCCCAACGAGGTCAAAGTTTTATACCATATCCTTTATATTTCTCTCGGCCTTGCTGGGTTGTTAATGGTTTTTATTATTTTGCAAAATCGTATCGGGTTTCATCAAATACAATACATCGGAAGTGCCATTGTTGTGATTGTTCTGCTTTTACTGCCTCTGGCCATTGTTTTCAGAGAGGAATTGATTGTTTGGAAGAGTAAAATCGGAAACCCAAATGCACAACTAGAGTTAGCATCTCAACAACCGCCGCCGCCACTTATCTCGGCAGTGCCACTGAGCCCTCCGTCGGACTCCTGCTTTAAAAACATGTTTAAACCCCCAAACAGAGGAGAGGACTACACAATCCCTCAAGCCATTTTCAGCGTCGATATGATCATTCTTTTTATCGCCTTAATCTGTGGTGTCGGTGGAACGTTGGCGGCGATGGACAATTTGGGTCAAATCAGCGAGTCTTTGGAGTACCCATCTCAGAGTACCACCACATTCATCTCTCTGGTCAGCATATGGAATTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTATTTCTCTTTGGGATGCTTATTTTATCCTGCGTCGGCTATCTTCTAATCGCATTCGGAGTTCCACATTCACTGTATGTTTCTTCAATCATAATTGGGTTCTGCTTTGGAGCGCAGTTGCCATTGATCTTGGCAATCATATCGGAAATTTTTGGGTTGAAGTACTATGCGACTCTAAGCAATTTGGGAGTAGCTGCAAACCCTATTGGGACGTATATTATGAATGTGAAAGTGGTGGGTCATTTGTACGACAGGGAGGCTGAACGGCAGATGGAGGCAGCAGGGGGACGAAGAAAAATAGGGGAGAATTTGAGTTGCTTGGGAGTGGAGTGTTACAGAAAGGCATTTCTTATAATCACGGGGACGACGGTGTTGGGAGGAGTTGTTTCCCTAATTTTAGTGGTTCGGACATGGAAGTTTTACAAGGGCGATATTTATAAGAAGTTCAGAGAGGAGGAAGGTGAAGACGTCGAGATCAAAACCACCGCATCGACCAAGACGCCTAAAACTGAATCTGTTTAG

Coding sequence (CDS)

ATGGCAACCTCCGGCAACCCCTTCTGGCATCAGTTGCTCTTTGGGCGGTGGTTCTCTGCCTTTGCCTCCATTCTTATGATGTCCGTCTCCGGGGCCGCCTTCATGTTTGCCCTCTACTCCTCCGACATCAAATATTCCTTTGGCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGCCTTAGGCGGCAATGCCGGCATCATCTGCGGCCTCATCAATGAGGTAGCTCCGACTTGGATGATTCTTCTAATCGGCGCCGTCATGAACCTTTTTGGCTACATCATGATTTGGCTAGCCGTAACCAAACGGATTCCCAAGCCCCAAATATGGCATATGTGTCTCTACATTTGCATCGGAGCTAATTCTCAGACCTTTACAAATACTGGGGCCATTGTAACCTTCGTCAATAACTTCCCGGAAAGTCGTGGTTGTGTTTTGGGACTTTTGAAGGGTTACGTCGGCTTAAGCGGCGCCATTTTGTCCCAGCTTTACCATGCCTTTTATGGCAACAATTCCAAATCTTTTATTCTCTTAATTGCTTGGCTTCCCGCCGCCATCACTGTTGTTTGCCTCCGTTTCGTTCGCATCATTAAGGATCTCCGGCTGCCCAACGAGGTCAAAGTTTTATACCATATCCTTTATATTTCTCTCGGCCTTGCTGGGTTGTTAATGGTTTTTATTATTTTGCAAAATCGTATCGGGTTTCATCAAATACAATACATCGGAAGTGCCATTGTTGTGATTGTTCTGCTTTTACTGCCTCTGGCCATTGTTTTCAGAGAGGAATTGATTGTTTGGAAGAGTAAAATCGGAAACCCAAATGCACAACTAGAGTTAGCATCTCAACAACCGCCGCCGCCACTTATCTCGGCAGTGCCACTGAGCCCTCCGTCGGACTCCTGCTTTAAAAACATGTTTAAACCCCCAAACAGAGGAGAGGACTACACAATCCCTCAAGCCATTTTCAGCGTCGATATGATCATTCTTTTTATCGCCTTAATCTGTGGTGTCGGTGGAACGTTGGCGGCGATGGACAATTTGGGTCAAATCAGCGAGTCTTTGGAGTACCCATCTCAGAGTACCACCACATTCATCTCTCTGGTCAGCATATGGAATTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTATTTCTCTTTGGGATGCTTATTTTATCCTGCGTCGGCTATCTTCTAATCGCATTCGGAGTTCCACATTCACTGTATGTTTCTTCAATCATAATTGGGTTCTGCTTTGGAGCGCAGTTGCCATTGATCTTGGCAATCATATCGGAAATTTTTGGGTTGAAGTACTATGCGACTCTAAGCAATTTGGGAGTAGCTGCAAACCCTATTGGGACGTATATTATGAATGTGAAAGTGGTGGGTCATTTGTACGACAGGGAGGCTGAACGGCAGATGGAGGCAGCAGGGGGACGAAGAAAAATAGGGGAGAATTTGAGTTGCTTGGGAGTGGAGTGTTACAGAAAGGCATTTCTTATAATCACGGGGACGACGGTGTTGGGAGGAGTTGTTTCCCTAATTTTAGTGGTTCGGACATGGAAGTTTTACAAGGGCGATATTTATAAGAAGTTCAGAGAGGAGGAAGGTGAAGACGTCGAGATCAAAACCACCGCATCGACCAAGACGCCTAAAACTGAATCTGTTTAG

Protein sequence

MATSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKALGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGANSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIAWLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQYIGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPLSPPSDSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYPSQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVPHSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHLYDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYKGDIYKKFREEEGEDVEIKTTASTKTPKTESV*
Homology
BLAST of Chy4G087530 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.1e-26
Identity = 124/543 (22.84%), Postives = 251/543 (46.22%), Query Frame = 0

Query: 13  LFGRWFS---AFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKALGGNAGI 72
           +FGRW       A+I + + +G  F F+ YSS +K   G  Q  LN L+    LG   G 
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 73  ICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYIC--IGANSQTF 132
             G+     P  ++L   A M   GY + WL +T  I  P   +  +++C  +   S  +
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICW 156

Query: 133 TNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIAWLPAA 192
            NT   +  + +FP +R   L L   + G+S A+ S  ++A   ++S  ++LL + +P  
Sbjct: 157 FNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLV 216

Query: 193 ITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMV----FIILQNRIGFHQIQYI 252
           ++   L  V     L    +     H  ++   L  L ++     ++  +     ++ +I
Sbjct: 217 VSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFI 276

Query: 253 GSAIVVIVLLLLPLAIVFREELI-VWKSKIGNPNAQLELASQQPPPPLISAVPLSPPSDS 312
           G+ ++++  L  PL +  R+  + V  +++ + ++   + +       +     S  S +
Sbjct: 277 GAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDE----LKNQKTSVSSKT 336

Query: 313 CFKNM--FKPPNR---GEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLE 372
            +++M   K  N    G++++    I  ++  + +IA  CG    L   +NLGQI++SL 
Sbjct: 337 GYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL- 396

Query: 373 YPSQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFG 432
              Q++TT +++ S +++ GR++S    ++  K++++ R  +    L+ + + + L+A  
Sbjct: 397 --GQNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVS 456

Query: 433 VPH--SLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKV 492
                +L  ++ +IG   G      ++I S++FG        N+ +   PIG+ +    +
Sbjct: 457 SSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-I 516

Query: 493 VGHLYDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTW 539
              +Y+  A   +        + +++ C+G +CY K F+     ++LG V SL L +RT 
Sbjct: 517 AASIYEANASPDITPI-----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTK 562

BLAST of Chy4G087530 vs. ExPASy TrEMBL
Match: A0A5A7TKH4 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002830 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 6.0e-263
Identity = 470/568 (82.75%), Postives = 503/568 (88.56%), Query Frame = 0

Query: 3   TSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKA 62
           TS NPFW+Q LFGRWFS FASIL+MSV+GA +MFALYSSDIK S GYDQTTLNLLSFFK 
Sbjct: 9   TSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKD 68

Query: 63  LGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGA 122
           LGGN G+I GLINEVAP W++LLIGAVMNLFGY MIWLAVT RIPKPQIWHMCLYIC+GA
Sbjct: 69  LGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGA 128

Query: 123 NSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIA 182
           NSQTF NTGA+VT V NFPESRG VLGLLKG+VGLSGAILSQL+HAFYGN+SKS ILLI 
Sbjct: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIG 188

Query: 183 WLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQY 242
           WLPAA++VV LRFVRIIKDLR PNEV V YHILYISL LAG LMV IILQ+ + F QIQY
Sbjct: 189 WLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRFQQIQY 248

Query: 243 IGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPL---SPP 302
           +GSAIVVIVLLLLPLAIVFREEL VWKSKI NP  QLELASQQPPPPL S VPL   SPP
Sbjct: 249 VGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLAPSSPP 308

Query: 303 SDSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYP 362
           SDSCFKNMFKPPNRGEDYTIPQAIFS+DMIILFIA ICGVGGTL A+DNLGQI ESL YP
Sbjct: 309 SDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP 368

Query: 363 SQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVP 422
           S STTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF  LILSC+G+LLIAFGVP
Sbjct: 369 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP 428

Query: 423 HSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHL 482
           +SLY SSIIIGFCFGAQ PLI AIISEIFGLKYYATL N+G AA+PIG YIMNV+V GHL
Sbjct: 429 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL 488

Query: 483 YDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYK 542
           YDREA+RQMEAAG  RK GE+LSCLGVECYRKAFLIIT  TV G +VSLILVVRTWKFYK
Sbjct: 489 YDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYK 548

Query: 543 GDIYKKFREEEGEDVEIKTTASTKTPKT 568
           GDIY++FR EEGED+E+K  A T +  T
Sbjct: 549 GDIYRRFRAEEGEDIEMKMAAPTNSTLT 576

BLAST of Chy4G087530 vs. ExPASy TrEMBL
Match: A0A1S3B711 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103486508 PE=4 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 6.6e-262
Identity = 482/535 (90.09%), Postives = 492/535 (91.96%), Query Frame = 0

Query: 1   MATSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFF 60
           MATSGNPFWHQL FGRWFSAFASILMMSVSGAAFMFAL SSDIK SFGYDQTT NLLSFF
Sbjct: 1   MATSGNPFWHQLFFGRWFSAFASILMMSVSGAAFMFALSSSDIKSSFGYDQTTXNLLSFF 60

Query: 61  KALGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICI 120
           K LGGNAG+I GLINEVAPTW ILLIGAVMNLFGY MIWLAVTK IP PQIWHMCLYICI
Sbjct: 61  KGLGGNAGVISGLINEVAPTWRILLIGAVMNLFGYTMIWLAVTKPIPNPQIWHMCLYICI 120

Query: 121 GANSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL 180
           GANSQTF NTGAIVT VNNFPESRG VLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL
Sbjct: 121 GANSQTFANTGAIVTCVNNFPESRGSVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL 180

Query: 181 IAWLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQI 240
           IAWLPAA+ VV LRFVRIIKDL  PNEVKV Y ILYISLGLAG L VFIILQNRI F QI
Sbjct: 181 IAWLPAAVIVVFLRFVRIIKDLLQPNEVKVFYRILYISLGLAGSLTVFIILQNRIRFQQI 240

Query: 241 QYIGSAIVVIVLLLLPL-AIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPLSPP 300
           QY+GSAIVVIVLLLLPL AIVFREELIVWK KI NP +QLELASQQ PPP ISAVPLSPP
Sbjct: 241 QYVGSAIVVIVLLLLPLAAIVFREELIVWKRKIANPISQLELASQQ-PPPRISAVPLSPP 300

Query: 301 SDSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYP 360
           SDSCFKNMFKPPNRGEDYTIPQAIFSVDM ILF+A IC VGGTL AMDNLGQI ES  YP
Sbjct: 301 SDSCFKNMFKPPNRGEDYTIPQAIFSVDMKILFMATICAVGGTLTAMDNLGQIGESSGYP 360

Query: 361 SQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPR-PLFLFGMLILSCVGYLLIAFGV 420
           SQSTTTFISLVSIWNYLGRVVSGFVSEY WKK+KVPR PLFLFG LI+SCVG+LLIAFGV
Sbjct: 361 SQSTTTFISLVSIWNYLGRVVSGFVSEYLWKKFKVPRPPLFLFGTLIISCVGHLLIAFGV 420

Query: 421 PHSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGH 480
           P+SLY S IIIGFCFGAQLPLILAI SEIFGLKYYATLSN GVAANPIGTYIMNVKVVGH
Sbjct: 421 PNSLYFSPIIIGFCFGAQLPLILAITSEIFGLKYYATLSNFGVAANPIGTYIMNVKVVGH 480

Query: 481 LYDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVR 534
           LYDREAERQMEAAGGRRKIGE+LSCLGVECYRKAFLIITGTTVLGGVVSLILVVR
Sbjct: 481 LYDREAERQMEAAGGRRKIGESLSCLGVECYRKAFLIITGTTVLGGVVSLILVVR 534

BLAST of Chy4G087530 vs. ExPASy TrEMBL
Match: A0A5A7TKY5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002840 PE=4 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 4.2e-256
Identity = 455/567 (80.25%), Postives = 495/567 (87.30%), Query Frame = 0

Query: 1   MATSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFF 60
           MATSGNPFW+ LLFGRWFS FASIL+MSVSGA +MF LYSS IK S  YDQTTLNLLSFF
Sbjct: 1   MATSGNPFWYHLLFGRWFSVFASILIMSVSGATYMFGLYSSHIKSSLAYDQTTLNLLSFF 60

Query: 61  KALGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICI 120
           K LG N G+I GL+NEVAP W++LLIG VMNLFGY MIWLAVT RIP PQIWHMCLYICI
Sbjct: 61  KDLGANIGVISGLVNEVAPAWVVLLIGVVMNLFGYTMIWLAVTNRIPNPQIWHMCLYICI 120

Query: 121 GANSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL 180
           GANSQTF NT A++T V NFPESRG +LGL KG+VGLSGAILSQL+HAFYGNNSKS I L
Sbjct: 121 GANSQTFANTAALITCVKNFPESRGSILGLFKGFVGLSGAILSQLFHAFYGNNSKSLIFL 180

Query: 181 IAWLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQI 240
           IAWLP+A++V+  RFVRIIKDLR PNE+KV YH+LYISLGLAG LMV IILQNR+ F QI
Sbjct: 181 IAWLPSAVSVILHRFVRIIKDLRQPNELKVFYHVLYISLGLAGSLMVCIILQNRLRFQQI 240

Query: 241 QYIGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPLSPPS 300
            Y+GSAIVVIVLLLLPLAIVFREEL +W+SKI NP  QLELASQ PPPP     P +PPS
Sbjct: 241 HYVGSAIVVIVLLLLPLAIVFREELRIWRSKIKNPIPQLELASQLPPPP-----PPNPPS 300

Query: 301 DSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYPS 360
           DSCFKNMF PPNRGEDYTIPQAIFS+DMIILFIA ICGVGGTL A+DNLGQI ESL YPS
Sbjct: 301 DSCFKNMFNPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYPS 360

Query: 361 QSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVPH 420
            STTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF  LILSC+G+LLIAFGVP+
Sbjct: 361 HSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVPN 420

Query: 421 SLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHLY 480
           SLY SSIIIGFCFGAQ PLI AIISEIFGLKYYATL N+G AA+PIG YIMNV+V GHLY
Sbjct: 421 SLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHLY 480

Query: 481 DREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYKG 540
           D EAERQMEAAG  RK GE+LSCLGVECY+KAFLIITG+TVLGG+VSLILVVRTWKFYK 
Sbjct: 481 DMEAERQMEAAGRLRKEGEDLSCLGVECYKKAFLIITGSTVLGGLVSLILVVRTWKFYKD 540

Query: 541 DIYKKFREEEGEDVEIKTTASTKTPKT 568
           DIY++F+E+EGED+EIK  A T    T
Sbjct: 541 DIYRRFKEKEGEDIEIKIAAPTNNSLT 562

BLAST of Chy4G087530 vs. ExPASy TrEMBL
Match: A0A0A0LEA4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G742600 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.9e-254
Identity = 455/563 (80.82%), Postives = 492/563 (87.39%), Query Frame = 0

Query: 3   TSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKA 62
           TS NPFW+Q LFGRWFS  ASIL+MSV+GA +MFALYSSDIK S GYDQTTLNLLSFFK 
Sbjct: 9   TSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKD 68

Query: 63  LGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGA 122
           LGGN G+I GL NEVAP W++LLIGAVMNLFGY MIWLAVTKRIPKPQIWHMCLYI IGA
Sbjct: 69  LGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGA 128

Query: 123 NSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIA 182
           NSQTF NTGA+VT V NFPESRG VLGLLKG+VGLSGAILSQLY AFYGNN +S ILLIA
Sbjct: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIA 188

Query: 183 WLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQY 242
           WLPAA++VV LRFVRIIKDLR PNE+KV YH LYISLGLAG LMV IILQ+ + F QIQY
Sbjct: 189 WLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQY 248

Query: 243 IGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAV---PLSPP 302
           +GSAIVVIVLLLLPL IVFREEL VWKSKI +P  QLE ASQQPPPPL S V   P SPP
Sbjct: 249 VGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLAPSSPP 308

Query: 303 SDSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYP 362
           S+SCFKNMF PP+RGEDYTIPQAIFS+D+IILF+A ICGVGGTL A+DNLGQI ESL Y 
Sbjct: 309 SESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQ 368

Query: 363 SQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVP 422
           S S TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF  LILSCVG+LLIAFGVP
Sbjct: 369 SHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVP 428

Query: 423 HSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHL 482
           +SLY SSI+IGFCFGAQ PLI AIISEIFGLKYYATLS++   A+PIG YI+NV+V GHL
Sbjct: 429 NSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHL 488

Query: 483 YDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYK 542
           YDREA+RQMEA G RR IGE+LSCLGVECYRKAFLIIT  TV G +VSLILVVRTWKFYK
Sbjct: 489 YDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYK 548

Query: 543 GDIYKKFREEEGEDVEIKTTAST 563
           GDIY+KFRE EGED+E+K  A T
Sbjct: 549 GDIYRKFREVEGEDIEMKMAAPT 571

BLAST of Chy4G087530 vs. ExPASy TrEMBL
Match: A0A6J1G7M8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451560 PE=4 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.6e-253
Identity = 450/567 (79.37%), Postives = 496/567 (87.48%), Query Frame = 0

Query: 6   NPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKALGG 65
           +P W+Q L GRWFS FASIL+MSVSGA +MF LYSSDIK S GYDQTTLNLLSFFK LGG
Sbjct: 10  HPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLLSFFKDLGG 69

Query: 66  NAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGANSQ 125
           N G+I GLINEVAP W++LLIGAVMNLFGY MIW+AVT RIPKPQIWHMCLYICIGANSQ
Sbjct: 70  NVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLYICIGANSQ 129

Query: 126 TFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIAWLP 185
           TF NTGA+VT V NFPESRG VLGLLKG+VGLSGAIL+QLYHAFYGNNSKS ILLIAWLP
Sbjct: 130 TFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSLILLIAWLP 189

Query: 186 AAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQYIGS 245
           AA++V  LRFVR+IKDLR PNE++V +HILYISLGLAG LMV IILQNR+ FHQ++Y GS
Sbjct: 190 AAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKFHQMEYAGS 249

Query: 246 AIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPL---SPPSDS 305
           AIVVI LLLLPLAIV+REEL VWK+KI +P +QLELASQQ PPPL SA P+   SPPS S
Sbjct: 250 AIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPVAPPSPPSGS 309

Query: 306 CFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYPSQS 365
           CF+N FKPPNRGEDYTIPQA+FS+DMIILFIA ICGVGGTL A+DNLGQI ESL YPSQS
Sbjct: 310 CFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYPSQS 369

Query: 366 TTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVPHSL 425
           TTTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F  +ILSCVG+LLIAFGVP+SL
Sbjct: 370 TTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLIAFGVPNSL 429

Query: 426 YVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHLYDR 485
           Y SSIIIGFCFGAQ PLI AIISEIFGLKYYATL NLG  A+PIG YI+NV+V GHLYDR
Sbjct: 430 YFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVRVAGHLYDR 489

Query: 486 EAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYKGDI 545
           EA RQMEAAG RR IGE+L+CLGVECYRKAFLIIT  TVLG  VSLILVVRTWKFYKGDI
Sbjct: 490 EATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRTWKFYKGDI 549

Query: 546 YKKFREEEGEDVEIKTTASTKTPKTES 570
           Y+KFREE GE+VE++  A T +   +S
Sbjct: 550 YRKFREEAGEEVEVQMAAPTNSAVAKS 576

BLAST of Chy4G087530 vs. NCBI nr
Match: KAA0043983.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25157.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 917 bits (2370), Expect = 0.0
Identity = 470/568 (82.75%), Postives = 503/568 (88.56%), Query Frame = 0

Query: 3   TSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKA 62
           TS NPFW+Q LFGRWFS FASIL+MSV+GA +MFALYSSDIK S GYDQTTLNLLSFFK 
Sbjct: 9   TSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKD 68

Query: 63  LGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGA 122
           LGGN G+I GLINEVAP W++LLIGAVMNLFGY MIWLAVT RIPKPQIWHMCLYIC+GA
Sbjct: 69  LGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGA 128

Query: 123 NSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIA 182
           NSQTF NTGA+VT V NFPESRG VLGLLKG+VGLSGAILSQL+HAFYGN+SKS ILLI 
Sbjct: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIG 188

Query: 183 WLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQY 242
           WLPAA++VV LRFVRIIKDLR PNEV V YHILYISL LAG LMV IILQ+ + F QIQY
Sbjct: 189 WLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRFQQIQY 248

Query: 243 IGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPL---SPP 302
           +GSAIVVIVLLLLPLAIVFREEL VWKSKI NP  QLELASQQPPPPL S VPL   SPP
Sbjct: 249 VGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLAPSSPP 308

Query: 303 SDSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYP 362
           SDSCFKNMFKPPNRGEDYTIPQAIFS+DMIILFIA ICGVGGTL A+DNLGQI ESL YP
Sbjct: 309 SDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP 368

Query: 363 SQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVP 422
           S STTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF  LILSC+G+LLIAFGVP
Sbjct: 369 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP 428

Query: 423 HSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHL 482
           +SLY SSIIIGFCFGAQ PLI AIISEIFGLKYYATL N+G AA+PIG YIMNV+V GHL
Sbjct: 429 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL 488

Query: 483 YDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYK 542
           YDREA+RQMEAAG  RK GE+LSCLGVECYRKAFLIIT  TV G +VSLILVVRTWKFYK
Sbjct: 489 YDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYK 548

Query: 543 GDIYKKFREEEGEDVEIKTTASTKTPKT 567
           GDIY++FR EEGED+E+K  A T +  T
Sbjct: 549 GDIYRRFRAEEGEDIEMKMAAPTNSTLT 576

BLAST of Chy4G087530 vs. NCBI nr
Match: XP_008442718.1 (PREDICTED: LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo])

HSP 1 Score: 914 bits (2361), Expect = 0.0
Identity = 482/535 (90.09%), Postives = 492/535 (91.96%), Query Frame = 0

Query: 1   MATSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFF 60
           MATSGNPFWHQL FGRWFSAFASILMMSVSGAAFMFAL SSDIK SFGYDQTT NLLSFF
Sbjct: 1   MATSGNPFWHQLFFGRWFSAFASILMMSVSGAAFMFALSSSDIKSSFGYDQTTXNLLSFF 60

Query: 61  KALGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICI 120
           K LGGNAG+I GLINEVAPTW ILLIGAVMNLFGY MIWLAVTK IP PQIWHMCLYICI
Sbjct: 61  KGLGGNAGVISGLINEVAPTWRILLIGAVMNLFGYTMIWLAVTKPIPNPQIWHMCLYICI 120

Query: 121 GANSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL 180
           GANSQTF NTGAIVT VNNFPESRG VLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL
Sbjct: 121 GANSQTFANTGAIVTCVNNFPESRGSVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL 180

Query: 181 IAWLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQI 240
           IAWLPAA+ VV LRFVRIIKDL  PNEVKV Y ILYISLGLAG L VFIILQNRI F QI
Sbjct: 181 IAWLPAAVIVVFLRFVRIIKDLLQPNEVKVFYRILYISLGLAGSLTVFIILQNRIRFQQI 240

Query: 241 QYIGSAIVVIVLLLLPLA-IVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPLSPP 300
           QY+GSAIVVIVLLLLPLA IVFREELIVWK KI NP +QLELASQQPPP  ISAVPLSPP
Sbjct: 241 QYVGSAIVVIVLLLLPLAAIVFREELIVWKRKIANPISQLELASQQPPP-RISAVPLSPP 300

Query: 301 SDSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYP 360
           SDSCFKNMFKPPNRGEDYTIPQAIFSVDM ILF+A IC VGGTL AMDNLGQI ES  YP
Sbjct: 301 SDSCFKNMFKPPNRGEDYTIPQAIFSVDMKILFMATICAVGGTLTAMDNLGQIGESSGYP 360

Query: 361 SQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRP-LFLFGMLILSCVGYLLIAFGV 420
           SQSTTTFISLVSIWNYLGRVVSGFVSEY WKK+KVPRP LFLFG LI+SCVG+LLIAFGV
Sbjct: 361 SQSTTTFISLVSIWNYLGRVVSGFVSEYLWKKFKVPRPPLFLFGTLIISCVGHLLIAFGV 420

Query: 421 PHSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGH 480
           P+SLY S IIIGFCFGAQLPLILAI SEIFGLKYYATLSN GVAANPIGTYIMNVKVVGH
Sbjct: 421 PNSLYFSPIIIGFCFGAQLPLILAITSEIFGLKYYATLSNFGVAANPIGTYIMNVKVVGH 480

Query: 481 LYDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVR 533
           LYDREAERQMEAAGGRRKIGE+LSCLGVECYRKAFLIITGTTVLGGVVSLILVVR
Sbjct: 481 LYDREAERQMEAAGGRRKIGESLSCLGVECYRKAFLIITGTTVLGGVVSLILVVR 534

BLAST of Chy4G087530 vs. NCBI nr
Match: XP_038905012.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 474/574 (82.58%), Postives = 502/574 (87.46%), Query Frame = 0

Query: 3   TSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKA 62
           TS NPFW+Q LFGRWFS FASIL+MSV+GA +MF LYSSDIK S GYDQTTLNLLSFFK 
Sbjct: 7   TSANPFWYQFLFGRWFSVFASILIMSVAGATYMFGLYSSDIKSSLGYDQTTLNLLSFFKD 66

Query: 63  LGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGA 122
           LGGN G+I GLINEVAP W++LLIGAVMNLFGY MIWLAVT RIPKPQIWHMCLYICIGA
Sbjct: 67  LGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICIGA 126

Query: 123 NSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIA 182
           NSQTF NTGA+VT V NFPESRG VLGLLKG+VGLSGAILSQL+HAFYGNNSKS I LIA
Sbjct: 127 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNNSKSLIFLIA 186

Query: 183 WLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQY 242
           WLPAA++V  LRFVRIIKDLR PNE+KV YHILYISLGLAG LMV IILQNR+ F QIQY
Sbjct: 187 WLPAAVSVTFLRFVRIIKDLRQPNELKVFYHILYISLGLAGSLMVLIILQNRLRFQQIQY 246

Query: 243 IGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPP--PPLISAVPLSPPS 302
           +GSAIVVIVLLLLPLAIVFREEL VWKSKI NP  QLELASQQPP  PPL    P SPPS
Sbjct: 247 VGSAIVVIVLLLLPLAIVFREELNVWKSKIANPILQLELASQQPPRPPPL---APPSPPS 306

Query: 303 DSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYPS 362
            SCFKN FKPPNRGEDYTIPQAIFSVDMIILFIA ICGVGGTL A+DNLGQI ESL YPS
Sbjct: 307 GSCFKNTFKPPNRGEDYTIPQAIFSVDMIILFIATICGVGGTLTAIDNLGQIGESLGYPS 366

Query: 363 QSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVPH 422
            STTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF  LILSCVG+LLIAFGVP+
Sbjct: 367 HSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPN 426

Query: 423 SLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHLY 482
           SLY SSI++GFCFGAQ PLI AIISEIFGLKYYATL NLG AA+PIG YI+NV+V GHLY
Sbjct: 427 SLYFSSIVVGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVRVAGHLY 486

Query: 483 DREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYKG 542
           DREA+RQMEAAG RR IGE+LSCLGVECYRKAFLIIT  TV GG+VSLILVVRTWKFYK 
Sbjct: 487 DREAQRQMEAAGRRRNIGEDLSCLGVECYRKAFLIITAATVFGGLVSLILVVRTWKFYKS 546

Query: 543 DIYKKFREEEGEDVEIK----TTASTKTPKTESV 570
           DIY+KFREEE EDVEIK       +    KTESV
Sbjct: 547 DIYRKFREEEAEDVEIKIVTPANGALTAAKTESV 577

BLAST of Chy4G087530 vs. NCBI nr
Match: KAA0043982.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25158.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 454/562 (80.78%), Postives = 494/562 (87.90%), Query Frame = 0

Query: 1   MATSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFF 60
           MATSGNPFW+ LLFGRWFS FASIL+MSVSGA +MF LYSS IK S  YDQTTLNLLSFF
Sbjct: 1   MATSGNPFWYHLLFGRWFSVFASILIMSVSGATYMFGLYSSHIKSSLAYDQTTLNLLSFF 60

Query: 61  KALGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICI 120
           K LG N G+I GL+NEVAP W++LLIG VMNLFGY MIWLAVT RIP PQIWHMCLYICI
Sbjct: 61  KDLGANIGVISGLVNEVAPAWVVLLIGVVMNLFGYTMIWLAVTNRIPNPQIWHMCLYICI 120

Query: 121 GANSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL 180
           GANSQTF NT A++T V NFPESRG +LGL KG+VGLSGAILSQL+HAFYGNNSKS I L
Sbjct: 121 GANSQTFANTAALITCVKNFPESRGSILGLFKGFVGLSGAILSQLFHAFYGNNSKSLIFL 180

Query: 181 IAWLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQI 240
           IAWLP+A++V+  RFVRIIKDLR PNE+KV YH+LYISLGLAG LMV IILQNR+ F QI
Sbjct: 181 IAWLPSAVSVILHRFVRIIKDLRQPNELKVFYHVLYISLGLAGSLMVCIILQNRLRFQQI 240

Query: 241 QYIGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPLSPPS 300
            Y+GSAIVVIVLLLLPLAIVFREEL +W+SKI NP  QLELASQ PPPP     P +PPS
Sbjct: 241 HYVGSAIVVIVLLLLPLAIVFREELRIWRSKIKNPIPQLELASQLPPPP-----PPNPPS 300

Query: 301 DSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYPS 360
           DSCFKNMF PPNRGEDYTIPQAIFS+DMIILFIA ICGVGGTL A+DNLGQI ESL YPS
Sbjct: 301 DSCFKNMFNPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYPS 360

Query: 361 QSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVPH 420
            STTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF  LILSC+G+LLIAFGVP+
Sbjct: 361 HSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVPN 420

Query: 421 SLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHLY 480
           SLY SSIIIGFCFGAQ PLI AIISEIFGLKYYATL N+G AA+PIG YIMNV+V GHLY
Sbjct: 421 SLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHLY 480

Query: 481 DREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYKG 540
           D EAERQMEAAG  RK GE+LSCLGVECY+KAFLIITG+TVLGG+VSLILVVRTWKFYK 
Sbjct: 481 DMEAERQMEAAGRLRKEGEDLSCLGVECYKKAFLIITGSTVLGGLVSLILVVRTWKFYKD 540

Query: 541 DIYKKFREEEGEDVEIKTTAST 562
           DIY++F+E+EGED+EIK  A T
Sbjct: 541 DIYRRFKEKEGEDIEIKIAAPT 557

BLAST of Chy4G087530 vs. NCBI nr
Match: XP_011651970.1 (uncharacterized protein LOC101216551 [Cucumis sativus] >KGN58997.1 hypothetical protein Csa_001993 [Cucumis sativus])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 455/563 (80.82%), Postives = 492/563 (87.39%), Query Frame = 0

Query: 3   TSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKA 62
           TS NPFW+Q LFGRWFS  ASIL+MSV+GA +MFALYSSDIK S GYDQTTLNLLSFFK 
Sbjct: 9   TSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKD 68

Query: 63  LGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGA 122
           LGGN G+I GL NEVAP W++LLIGAVMNLFGY MIWLAVTKRIPKPQIWHMCLYI IGA
Sbjct: 69  LGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGA 128

Query: 123 NSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIA 182
           NSQTF NTGA+VT V NFPESRG VLGLLKG+VGLSGAILSQLY AFYGNN +S ILLIA
Sbjct: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIA 188

Query: 183 WLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQY 242
           WLPAA++VV LRFVRIIKDLR PNE+KV YH LYISLGLAG LMV IILQ+ + F QIQY
Sbjct: 189 WLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQY 248

Query: 243 IGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAV---PLSPP 302
           +GSAIVVIVLLLLPL IVFREEL VWKSKI +P  QLE ASQQPPPPL S V   P SPP
Sbjct: 249 VGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLAPSSPP 308

Query: 303 SDSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYP 362
           S+SCFKNMF PP+RGEDYTIPQAIFS+D+IILF+A ICGVGGTL A+DNLGQI ESL Y 
Sbjct: 309 SESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQ 368

Query: 363 SQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVP 422
           S S TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF  LILSCVG+LLIAFGVP
Sbjct: 369 SHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVP 428

Query: 423 HSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHL 482
           +SLY SSI+IGFCFGAQ PLI AIISEIFGLKYYATLS++   A+PIG YI+NV+V GHL
Sbjct: 429 NSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHL 488

Query: 483 YDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYK 542
           YDREA+RQMEA G RR IGE+LSCLGVECYRKAFLIIT  TV G +VSLILVVRTWKFYK
Sbjct: 489 YDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYK 548

Query: 543 GDIYKKFREEEGEDVEIKTTAST 562
           GDIY+KFRE EGED+E+K  A T
Sbjct: 549 GDIYRKFREVEGEDIEMKMAAPT 571

BLAST of Chy4G087530 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 641.3 bits (1653), Expect = 7.1e-184
Identity = 334/567 (58.91%), Postives = 418/567 (73.72%), Query Frame = 0

Query: 11  QLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKALGGNAGII 70
           Q+L GRWF  F S+L+MS +GA +MF +YS DIK + GYDQTTLNLLSFFK LG N G++
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 71  CGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGANSQTFTNT 130
            GL+NEV P W ILLIGA++N FGY MIWLAVT+RI KPQ+WHMCLYIC+GANSQ+F NT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 131 GAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIAWLPAAITV 190
           G++VT V NFPESRG VLG+LKGYVGLSGAI++QLY AFYG ++K  IL+I WLPA ++ 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSF 195

Query: 191 VCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQYIGSAIVVI 250
             LR +RI+K  R  NE+KV Y+ LYISLGLA  LMV II+    GF Q ++ GSA VVI
Sbjct: 196 AFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVI 255

Query: 251 VLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISA------------VPLSP 310
           VLLLLP+ +V  EE  +WK K    N    +      P L S+            V    
Sbjct: 256 VLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEKV 315

Query: 311 PSDSCFKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEY 370
            + SC+  +F PP RG+DYTI QA+FSVDM+ILF+A ICGVGGTL A+DNLGQI  SL Y
Sbjct: 316 KTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGY 375

Query: 371 PSQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGV 430
           P +S +TF+SLVSIWNY GRVVSG VSE F  KYK PRPL L  +L+LSC G+LLIAF V
Sbjct: 376 PKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNV 435

Query: 431 PHSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGH 490
           P  LYV+S+IIGFCFGAQ PL+ AIISEIFGLKYY+TL N G  A+PIG+Y++NV+V G+
Sbjct: 436 PGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGY 495

Query: 491 LYDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFY 550
           LYD EA +Q +A G  R  G++L+C+G  C++ +F+II   T+ G +VS++LV+RT KFY
Sbjct: 496 LYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFY 555

Query: 551 KGDIYKKFREEE-GEDVEIKTTASTKT 565
           K DIYKKFRE+    ++E+   A+ ++
Sbjct: 556 KSDIYKKFREKALAAEMEMAAPAAARS 582

BLAST of Chy4G087530 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 587.8 bits (1514), Expect = 9.4e-168
Identity = 306/553 (55.33%), Postives = 392/553 (70.89%), Query Frame = 0

Query: 15  GRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKALGGNAGIICGLI 74
           GRWF  FAS L+M+ +GA ++F  YS DIK + GYDQTTLNLL FFK LG N G++ GLI
Sbjct: 16  GRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLI 75

Query: 75  NEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGANSQTFTNTGAIV 134
            EV PTW +L IG+ MN  GY MIWL VT ++ KP++W MCLYICIGANSQ F NTGA+V
Sbjct: 76  AEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALV 135

Query: 135 TFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILLIAWLPAAITVVCLR 194
           T V NFPESRG +LGLLKGYVGLSGAI +QLY A YG++SKS ILLIAWLPAA+++V + 
Sbjct: 136 TCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVY 195

Query: 195 FVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQYIGSAIVVIVLLL 254
            +R  K +R  NE+ V Y  LYIS+ LA  LM   I + ++ F +  Y  SA +   LL 
Sbjct: 196 LIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLF 255

Query: 255 LPLAIVFREELIVW-----------KSKIGNPNAQLELASQQPPPPLISAVPLSPPSDSC 314
           +PL +  ++EL VW           + K+  P  +L+L   Q     ++       + SC
Sbjct: 256 VPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDL--DQDKAAKVNGE--EKETKSC 315

Query: 315 FKNMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYPSQST 374
           F  +F PP RGEDYTI QA+ S DMIILF+A  CG+G +L A+DNLGQI ESL YP+ + 
Sbjct: 316 FSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTV 375

Query: 375 TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVPHSLY 434
           ++F+SLVSIWNY GRV SGFVSEY   KYK+PRPL +  +L+LSC G+LLIAF VP S+Y
Sbjct: 376 SSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVY 435

Query: 435 VSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHLYDRE 494
           ++SI++GF FGAQLPL+ AIISE+FGLKYY+TL N G  A+P+G+YI+NV+V G LYD+E
Sbjct: 436 IASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKE 495

Query: 495 AERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYKGDIY 554
           A +Q+ A G  RK  ++L+CLG +CY+  FLI+   T  G +VSL L +RT +FYKGDIY
Sbjct: 496 ALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIY 555

Query: 555 KKFREEEGEDVEI 557
           KKFRE    + E+
Sbjct: 556 KKFRESPESESEL 564

BLAST of Chy4G087530 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 1.3e-84
Identity = 193/540 (35.74%), Postives = 308/540 (57.04%), Query Frame = 0

Query: 16  RWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKALGGNAGIICGLIN 75
           +W +A ASI + S SGA + FA+YSS +K S  YDQ+TL+ +S FK +GG  GII G + 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 76  EVAPT--------WMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICIGANSQTF 135
               +        W+++ +G V    G+  IW +V   I  P +  MCL++ +  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 136 TNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYG--NNSKSFILLIAWLP 195
            NT  +VT   NF +  G  +G+++G++GLSGAIL QLYHA  G   N  +FILL+A +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 196 AAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGFHQIQYIGS 255
             +  + + FVR+ + + + ++ K L  +  IS+ +A  LMV I ++N +G  +   I S
Sbjct: 187 TLVMFLAMPFVRVYETVTI-SDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFS 246

Query: 256 AIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAVPLSPPSDSCFK 315
            I+V++LL  PL +  R               + +  S    P L ++  L PPS + F 
Sbjct: 247 FILVLLLLASPLLVAVRA-----------LREKRQTLSSLDGPVLDTSALLDPPSSNIFP 306

Query: 316 NMFKPPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYPSQSTTT 375
           +        ED  I +A+ +V+  +LF+A++CG+G   A ++N+ QI ESL Y S    +
Sbjct: 307 D--GDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNS 366

Query: 376 FISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVPHSLYVS 435
            +SL SIWN+LGR  +G+VS+ F  K+  PRP+F+   L +  +G++++A GV  SLY  
Sbjct: 367 LVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAG 426

Query: 436 SIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHLYDREAE 495
           S++IG  +G+Q  L+  I SEIFG+++  T+      A PIG+YI++VKV+G+ YD+ A 
Sbjct: 427 SVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVAS 486

Query: 496 RQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYKGDIYKK 546
                        ++ SC G +C+R +F+I+    + G +V+ +L  RT KFYK  + K+
Sbjct: 487 E------------DDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

BLAST of Chy4G087530 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 6.0e-82
Identity = 188/552 (34.06%), Postives = 305/552 (55.25%), Query Frame = 0

Query: 11  QLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFFKALGGNAGII 70
           ++L  +W +  ASI +   SGA++ F +YS+ +K +  YDQ+TL+ +S FK +G NAG+ 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 71  CGLINEVAPT----------------WMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHM 130
            GL+   A +                W++L +GA+    GY +IW +VT  I KP +  M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 131 CLYICIGANSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNS 190
           CL++ + A SQTF NT  +V+ V NF +  G  +G++KG++GLSGAIL QLY      + 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 191 KSFILLIAWLPAAITVVCLRFVRIIKDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNR 250
            SFILL+A  P  ++++ +  VRI  +  + ++ K L  +  +SL +A  LM+ IIL+N 
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIY-ETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNT 241

Query: 251 IGFHQIQYIGSAIVVIVLLLLPLAIVFREELIVWKSKIGNPNAQLELASQQPPPPLISAV 310
            G      I + + ++V+L LPL I  R +              +E        PLIS+ 
Sbjct: 242 FGLSSWANIVTLVCLLVMLALPLLIARRAQ-----------RDGMEKTVPHDYSPLISSP 301

Query: 311 PLSPPSDSCFKNMFK-PPNRGEDYTIPQAIFSVDMIILFIALICGVGGTLAAMDNLGQIS 370
             +   +   +   K      E+  + QA+  +   +LF+A+ICG+G  L+ ++N+ QI 
Sbjct: 302 KATTSGNQSSEGDSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIG 361

Query: 371 ESLEYPSQSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFGMLILSCVGYLL 430
           ESL Y S    + +SL SIWN+LGR  +G+ S+    K   PRPL +   L    +G+L+
Sbjct: 362 ESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLI 421

Query: 431 IAFGVPHSLYVSSIIIGFCFGAQLPLILAIISEIFGLKYYATLSNLGVAANPIGTYIMNV 490
           IA G   +LYV S+I+G C+G+Q  L+  I SE+FG+++  T+ N    A+PIG+YI +V
Sbjct: 422 IASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSV 481

Query: 491 KVVGHLYDREAERQMEAAGGRRKIGENLSCLGVECYRKAFLIITGTTVLGGVVSLILVVR 546
           +++G++YD+ A             GE  +C G  C+R +F+I+      G +V+++L  R
Sbjct: 482 RLIGYIYDKTAS------------GEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFR 529

BLAST of Chy4G087530 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 5.1e-81
Identity = 200/586 (34.13%), Postives = 312/586 (53.24%), Query Frame = 0

Query: 1   MATSGNPFWHQLLFGRWFSAFASILMMSVSGAAFMFALYSSDIKYSFGYDQTTLNLLSFF 60
           MA++    +   +  RW    A++ + S +G  ++F   S  IK S  Y+Q  L+ L   
Sbjct: 1   MASTTREKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVA 60

Query: 61  KALGGNAGIICGLINEVAPTWMILLIGAVMNLFGYIMIWLAVTKRIPKPQIWHMCLYICI 120
           K LG + G I G ++E+ P W  LL+GAV NL GY  +WL VT R P   +W MC+ I +
Sbjct: 61  KDLGDSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFV 120

Query: 121 GANSQTFTNTGAIVTFVNNFPESRGCVLGLLKGYVGLSGAILSQLYHAFYGNNSKSFILL 180
           G N +T+ NTGA+V+ V NFP+SRG V+G+LKG+ GL GAI+SQ+Y   + +N  S IL+
Sbjct: 121 GNNGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILM 180

Query: 181 IAWLPAAITVVCLRFVRII---KDLRLPNEVKVLYHILYISLGLAGLLMVFIILQNRIGF 240
           +A  PA + V  + F+R +   K +R P +      I  + L LA  LM  +++Q+ +  
Sbjct: 181 VAVTPAVVVVCLMFFIRPVGGHKQIR-PTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVV 240

Query: 241 HQIQYIGSAIVVIVLLLLPLAIVFR--------------EELIVWKSKIGNPNAQ---LE 300
                    IV+ V+L++P+ +                 EE +V K +   P  Q   L 
Sbjct: 241 SHNVITVFTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLI 300

Query: 301 LASQQPPPPLISAVPLSPPSD-------------------SCFKNMFKPPNRGEDYTIPQ 360
           L+  +   P    V L P S+                   +   N  + P+RGED+T+ Q
Sbjct: 301 LSEVEDEKP--KDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQ 360

Query: 361 AIFSVDMIILFIALICGVGGTLAAMDNLGQISESLEYPSQSTTTFISLVSIWNYLGRVVS 420
           A+   D  ++F +L+ G G  L  +DNLGQ+S+SL Y   +T   +S++SIWN+LGR+  
Sbjct: 361 ALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGG 420

Query: 421 GFVSEYFWKKYKVPRPLFLFGMLILSCVGYLLIAFGVPHSLYVSSIIIGFCFGAQLPLIL 480
           G+ SE   + Y  PRP+ +    ++  VG++  A+G P ++Y+ +++IG  +GA   ++ 
Sbjct: 421 GYFSELVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVP 480

Query: 481 AIISEIFGLKYYATLSNLGVAANPIGTYIMNVKVVGHLYDREAERQMEAAGGRRKIGENL 540
           A  SE+FGLK +  L N    ANP G+ + +  +   +YDREAERQ  A G      + L
Sbjct: 481 ATASELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDAL 540

Query: 541 SCLGVECYRKAFLIITGTTVLGGVVSLILVVRTWKFYKGDIYKKFR 548
            C G  C+    LI++G  ++  ++S+ILV RT   Y   +Y K R
Sbjct: 541 RCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVYT-HLYGKTR 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E12.1e-2622.84Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A5A7TKH46.0e-26382.75Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B7116.6e-26290.09LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=... [more]
A0A5A7TKY54.2e-25680.25Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A0A0LEA43.9e-25480.82Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G74260... [more]
A0A6J1G7M85.6e-25379.37protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
KAA0043983.10.082.75protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25157.1 p... [more]
XP_008442718.10.090.09PREDICTED: LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis... [more]
XP_038905012.10.082.58protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
KAA0043982.10.080.78protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25158.1 p... [more]
XP_011651970.10.080.82uncharacterized protein LOC101216551 [Cucumis sativus] >KGN58997.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
AT2G39210.17.1e-18458.91Major facilitator superfamily protein [more]
AT2G28120.19.4e-16855.33Major facilitator superfamily protein [more]
AT2G34355.11.3e-8435.74Major facilitator superfamily protein [more]
AT1G74780.16.0e-8234.06Nodulin-like / Major Facilitator Superfamily protein [more]
AT5G14120.15.1e-8134.13Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 321..537
e-value: 2.6E-11
score: 45.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 216..536
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 13..203
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 16..261
e-value: 3.2E-83
score: 279.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 550..570
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 6..563
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 6..563
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 15..523
e-value: 5.92026E-101
score: 308.797

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G087530.1Chy4G087530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0099587 inorganic ion import across plasma membrane
biological_process GO:0002239 response to oomycetes
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity