CmaCh20G002010 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G002010
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Mannan endo-1,4-beta-mannosidase
Location	Cma_Chr20: 950019 .. 954124 (-)
RNA-Seq Expression	CmaCh20G002010
Synteny	CmaCh20G002010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAGTTAACAATGCTTAAGAATTTAACTAAATAAAGGGTGTTGTTTCTTAGGAGGAAATAGCTTAAATCTTTCCATATTTATTCACTTCTTACGATGCTCGCGTATCTGTGTGGACTGTACTATTTGTTATAACCCTTTGCATTGTTTTGATTACTTATTGTTCTGACGCATAAGGACTAAGATTCTTAGATGAAATAGCTTAAAAAAATGGAAATCATGGTGGGAGACAAGGAGAAACTCGAGCATGTGAGTGATTCAGAAGCAGGTTTTTGAGATCCACTGCAGTGGTAAGGTGATCTTTGTCTGTATATTGGAAGGGGGGAAGATTTGGAAGCAGCCCATTTACGTGGGTTGAAGAACAAGACTATTGCGTTCTTGGGTCGTGATCGAAAAGGCTGAAAACCATGAAAAGATACCAACATTCTACCATACTCCCTCAACCTCTTTTTACTTCTTTTGCTTAATCTCTCATTAATTCTTCTTCACTGCTTATTTGGGCTGCAGATTTTGGTGGGGTTTTCTTCAATCATAACCTCTCTGTTCCTTCTCTCTTATAAATCCCCAAAACCCAAGATTGCAATCAGCTTGAAGTGGGAGTGTTACTATTCATAACACGTTCCAACTCCACGAAGAATCGTTGGCATACATTTGTGGTAGAAATAGGGATCTGGGTTTACAATAAGATAGGCCAATTTAGCCGACATTCTTGTTTCTTGTGGTTTAGTGTTCTGAAATCGCATCTGGGTATGAGCGATTCTTTGATTTTGTTTGATATATTGTCATAAACAGTTGAGTTTTGTTTTTTACAATCGTTTCGTTTTGTATTTTGGGGTTTCCATCGCCGCCTATATTGTGTTCGTAGGGAGGAAATAATGGCGGGGAATGGGCTCTTTTACCCGATTCTTGGACTCGCATGTTGTTTGGCTTTCATTTACATATCGTTTGGGGACTGGAAGCTTGGGGATACTAAAGCCCCCAAAATGGGTTTTGTAGAGAGGAATGGAACGAAGTTCATGGTGAATGGGAGGGCGTTTTATATCAACGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGGACATAGAAAGCCCAGAGTCAGAGCGATGCTGCGGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGAACTTGGGCCTTCAGCGACGGTGGCTACAATGCCCTCCAGGTTTTTCCCGGCAGGTTTAACGAGCAAGTCTTTAAGGTAAGTGAAACGTCTGTTGCTGTGATGAGCTATATTTTTCTTAGAGATTCCTGTTGTTTCTGTGATTGGGTTCTTTACTTGTTGAGCTCTTGTTTGATTCCTACTCTCATGTGCCTTGGTTCTGTCTTTGTGATTGGCTTCTTCTGAAATTCTATGTCAATGTCTGTTTTAGAGAGCGATGAAGTGTGTCTGCAGGCGTTGGAAGGCTCCACTGGCCAAAAAATGGAAGCTGGGTGCTTCCACCGACAGAAGAATTGTGTGAATATGATTTGAATTCTCACGTTCTATAAGCCGATTGAAAAACATTATTACTTATATTTCTATGAATGCTCAAGGTGGTTTTGTTTGTTTACTTCTTCATATAGTTCTAAGTTCATAAATTGAGGACTGGAGTTCATGGACGAACTAGATAACTTCCTATTACTAACGGTTGGAGAAGGGAACGAAACATTTCTTATAAGGGTGTGAAAACCTCTCCCTAACACTTTTTAAAACCTTTAGGGGAAGCCCAGAAGAGAAAGACCAAAGATGACAATATCGACTAGCGGTGTTCTTGGGCTGTTATAAATGGTATCAGAGTCAGACACTAGGCGGTGTGCCAACGAGGACGTTGGAGCCTTAAGAAGGGTGGATGATGAGATCCCACATCGGTTGGAGAGGGGAATGAAGCATTCCTTCCTATAAGGGTATGAAAACCTCCTCCTAACAAACGCGCTTTAAAACTTTGGGAAAAGCTCAAAGAAGACAATATTTACTAGTGGTGAGCTTGGGCGATTACCATGTCTCTCTCTCTTGTTTCTAAAATGACACATTATTCAAACTACAAACAATAAAATATATGCATATATGTTTTCGTGATTTGTTTGGTTTGACGATAATTTCAATTTGCTCAAGTTGTCGGTTAACAGCTGAAGTTTTAACTGGTTTTTCCTGGTGGCAGGCTTTGGATCATGTCATTGCAGAATCAAGACAACATGGGATAAGGCTTCTCCTTAGCTTAGTCAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCGATACGCATTTATTTTAAGAATTATATTAAGGTACGTTCCATATTTATCACTTAGTTTGTCCGGTATGGTTCGTAACGCTAATGATTTGGGTATTCATCCTGAATTTATGCTGTGGATTTTGAGTGTGAGATCCCATATCAGCTGGAGAGGTGAATGAAACATTTCTTATAAGGGTGTGGAAACCTCTCCCTAACAGACGCGTTTTAAAACCTTGAGGGGAAGCCCAGAAGGGAAAACCCAAAAAGGACAATATATGCTAACGGTGGACTTGAGCTGTTACAAATGGTATCAGAGCTAGACACTGAGCGGTGTGCTAGCGAGGATGCTAGGCCCCCAAAGGGAGTGGATTGTGAGATCTCATATCGACTGGAGAGGGGAACGGAACATTTCTTATGAGGGTGTAAAAACCTCTATCTGACAGACATGTTTTAAACCTTGAGGAGAAGCCCAAAAAAGAAAGTCTCAAAAGAACAATATCTGCTAGCACCAAACAAAAATGATTTCCCTTGTCTTAGCTGTTCTTACATTGCTACCTTATGGTGTTTACTCGTTCTAATGTGCACTACACTTTCCAGACTGTCCTAACAAGAAGGAACTCCATCACGGGAATTGAATATAGAAACGATCCGACCATCTTCGGATGGGAATTGATTAACGAGCCACGTTGTATGACCGATGCCTCAGGCGATACTCTTCAAGTAAGTTGATACTCCTCAATATAGTTGTAAGATTCTGATTCTTCTGGCTTCTGAATGTTAAACCAAATGAAAACTGAACTTGTAGGAGTGGATTGAAGAAATGTCAGCTTACATTAAATCGATAGACAAGAAGCATCTATTAACTGTTGGGTTAGAAGGATTTTATGGTCCAAATAGCCCCAAGAAATCTACTGCGAACCCGGAAGAGTGGGCATCCAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCACATATAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTAAGCATTGAATGATAGTACTCAATGATTCAGCTCATGTCTATGCTCTTAAACCCGTTACTTATACGATGAAATTAACATTCCGTGTTGATTAGGTTCCATGATCAAGATTTTGAAGACGAACTACAATTTGTATCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACAAAGAGTTGAAGAAACCAGTGATGTTTACTGAATTTGGACTGTCAGACCTGAACAAAGGCTTCACCCCAGCTCAGCGGGATAGATTTTACAAAACTGTTTACGACATCATTTACGAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCCTGGCAGTTCCTTGTTGAAGGCATGGAAGAGAGTAATGACGATTTCGGGATAGTCCCGTGGGAAAGATCGTCGATATATCGGCTTATTATAGAGCAATCATGCAGGTTGGCAAGGATTGGTGGAGACATCCAACAGTTAAAAGCATTGAAAAATATGTGCTGAGAGATGGTGATTTGAATATTACTTATTTGAGAGAATTCAATTTGTTCCCTTCCTGCTATATTTTGGCTCTCTGCCATTTGTTTCTTCTGTTCATACATATTTGAAATAGATTAGTTAGTTTAACCTATAGGAGTGAGTTTCAACCAGACTAAACATGAACTGGTAGAAGCTGTAGTGTTTTTTGAGCGAATATACAAGCCAGTACATGAACAACAAGCTGTAATGTTTTTGAGTAAATATACGAGTCATAATCTATTTTACCTTTGAAGTTCTCTCCAAGGCTGCAG

mRNA sequence

ATAGTTAACAATGCTTAAGAATTTAACTAAATAAAGGGTGTTGTTTCTTAGGAGGAAATAGCTTAAATCTTTCCATATTTATTCACTTCTTACGATGCTCGCGTATCTGTGTGGACTGTACTATTTGTTATAACCCTTTGCATTGTTTTGATTACTTATTGTTCTGACGCATAAGGACTAAGATTCTTAGATGAAATAGCTTAAAAAAATGGAAATCATGGTGGGAGACAAGGAGAAACTCGAGCATGTGAGTGATTCAGAAGCAGGTTTTTGAGATCCACTGCAGTGGTAAGGTGATCTTTGTCTGTATATTGGAAGGGGGGAAGATTTGGAAGCAGCCCATTTACGTGGGTTGAAGAACAAGACTATTGCGTTCTTGGGTCGTGATCGAAAAGGCTGAAAACCATGAAAAGATACCAACATTCTACCATACTCCCTCAACCTCTTTTTACTTCTTTTGCTTAATCTCTCATTAATTCTTCTTCACTGCTTATTTGGGCTGCAGATTTTGGTGGGGTTTTCTTCAATCATAACCTCTCTGTTCCTTCTCTCTTATAAATCCCCAAAACCCAAGATTGCAATCAGCTTGAAGTGGGAGTGTTACTATTCATAACACGTTCCAACTCCACGAAGAATCGTTGGCATACATTTGTGGTAGAAATAGGGATCTGGGTTTACAATAAGATAGGCCAATTTAGCCGACATTCTTGTTTCTTGTGGTTTAGTGTTCTGAAATCGCATCTGGGGAGGAAATAATGGCGGGGAATGGGCTCTTTTACCCGATTCTTGGACTCGCATGTTGTTTGGCTTTCATTTACATATCGTTTGGGGACTGGAAGCTTGGGGATACTAAAGCCCCCAAAATGGGTTTTGTAGAGAGGAATGGAACGAAGTTCATGGTGAATGGGAGGGCGTTTTATATCAACGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGGACATAGAAAGCCCAGAGTCAGAGCGATGCTGCGGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGAACTTGGGCCTTCAGCGACGGTGGCTACAATGCCCTCCAGGTTTTTCCCGGCAGGTTTAACGAGCAAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGACAACATGGGATAAGGCTTCTCCTTAGCTTAGTCAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCGATACGCATTTATTTTAAGAATTATATTAAGACTGTCCTAACAAGAAGGAACTCCATCACGGGAATTGAATATAGAAACGATCCGACCATCTTCGGATGGGAATTGATTAACGAGCCACGTTGTATGACCGATGCCTCAGGCGATACTCTTCAAGAGTGGATTGAAGAAATGTCAGCTTACATTAAATCGATAGACAAGAAGCATCTATTAACTGTTGGGTTAGAAGGATTTTATGGTCCAAATAGCCCCAAGAAATCTACTGCGAACCCGGAAGAGTGGGCATCCAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCACATATAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCATGATCAAGATTTTGAAGACGAACTACAATTTGTATCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACAAAGAGTTGAAGAAACCAGTGATGTTTACTGAATTTGGACTGTCAGACCTGAACAAAGGCTTCACCCCAGCTCAGCGGGATAGATTTTACAAAACTGTTTACGACATCATTTACGAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCCTGGCAGTTCCTTGTTGAAGGCATGGAAGAGAGTAATGACGATTTCGGGATAGTCCCGTGGGAAAGATCGTCGATATATCGGCTTATTATAGAGCAATCATGCAGGTTGGCAAGGATTGGTGGAGACATCCAACAGTTAAAAGCATTGAAAAATATGTGCTGAGAGATGGTGATTTGAATATTACTTATTTGAGAGAATTCAATTTGTTCCCTTCCTGCTATATTTTGGCTCTCTGCCATTTGTTTCTTCTGTTCATACATATTTGAAATAGATTAGTTAGTTTAACCTATAGGAGTGAGTTTCAACCAGACTAAACATGAACTGGTAGAAGCTGTAGTGTTTTTTGAGCGAATATACAAGCCAGTACATGAACAACAAGCTGTAATGTTTTTGAGTAAATATACGAGTCATAATCTATTTTACCTTTGAAGTTCTCTCCAAGGCTGCAG

Coding sequence (CDS)

ATGGCGGGGAATGGGCTCTTTTACCCGATTCTTGGACTCGCATGTTGTTTGGCTTTCATTTACATATCGTTTGGGGACTGGAAGCTTGGGGATACTAAAGCCCCCAAAATGGGTTTTGTAGAGAGGAATGGAACGAAGTTCATGGTGAATGGGAGGGCGTTTTATATCAACGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGGACATAGAAAGCCCAGAGTCAGAGCGATGCTGCGGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGAACTTGGGCCTTCAGCGACGGTGGCTACAATGCCCTCCAGGTTTTTCCCGGCAGGTTTAACGAGCAAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGACAACATGGGATAAGGCTTCTCCTTAGCTTAGTCAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCGATACGCATTTATTTTAAGAATTATATTAAGACTGTCCTAACAAGAAGGAACTCCATCACGGGAATTGAATATAGAAACGATCCGACCATCTTCGGATGGGAATTGATTAACGAGCCACGTTGTATGACCGATGCCTCAGGCGATACTCTTCAAGAGTGGATTGAAGAAATGTCAGCTTACATTAAATCGATAGACAAGAAGCATCTATTAACTGTTGGGTTAGAAGGATTTTATGGTCCAAATAGCCCCAAGAAATCTACTGCGAACCCGGAAGAGTGGGCATCCAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCACATATAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCATGATCAAGATTTTGAAGACGAACTACAATTTGTATCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACAAAGAGTTGAAGAAACCAGTGATGTTTACTGAATTTGGACTGTCAGACCTGAACAAAGGCTTCACCCCAGCTCAGCGGGATAGATTTTACAAAACTGTTTACGACATCATTTACGAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCCTGGCAGTTCCTTGTTGAAGGCATGGAAGAGAGTAATGACGATTTCGGGATAGTCCCGTGGGAAAGATCGTCGATATATCGGCTTATTATAGAGCAATCATGCAGGTTGGCAAGGATTGGTGGAGACATCCAACAGTTAAAAGCATTGAAAAATATGTGCTGA

Protein sequence

MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWNSYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYESAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLKALKNMC

Homology

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BLAST of CmaCh20G002010 vs. ExPASy Swiss-Prot
Match: Q7Y223 (Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 4.8e-198
Identity = 314/412 (76.21%), Postives = 363/412 (88.11%), Query Frame = 0


Query: 3   GNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWNSY 62
           GNG   PILG   C+AFIY+SFGD   G     ++ FV+RNGT+F+V+G+A Y+NGWNSY
Sbjct: 6   GNGPVIPILGFLTCVAFIYLSFGDLWFGLKTEGELAFVKRNGTQFVVDGKALYVNGWNSY 65

Query: 63  WFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDH 122
           WFMDHAV  H + RV AML A  KMGLTVCRTWAF+DGGYNALQ+ PGRF+E+VFKALDH
Sbjct: 66  WFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFKALDH 125

Query: 123 VIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNY 182
           VIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQ+GVGLSSSNDSFF+DPSIR YFKNY
Sbjct: 126 VIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSNDSFFFDPSIRRYFKNY 185

Query: 183 IKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKH 242
           +  +LTR+NS+TGIEYRNDPTIF WELINEPRCM+D SGDTLQ+WI EM+A+IKSID KH
Sbjct: 186 LTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDWINEMTAFIKSIDNKH 245

Query: 243 LLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDF 302
           LLTVGLEGFYGP+SPKK T NPE WAS LGSDF+RNS+ P+IDFASVHIYPDHWFHDQ F
Sbjct: 246 LLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVHIYPDHWFHDQGF 305

Query: 303 EDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYESA 362
           E++L+FV KWMLSHIEDGDKELKKPV+FTEFGLS+LNK + P+QRDRFY+T++D+IY+SA
Sbjct: 306 EEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKSA 365

Query: 363 KRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGG 415
           KRKRSGAG+L WQFL+EGME  NDDFGIVPWE+ SI RL+IEQSCRL+RI G
Sbjct: 366 KRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITG 417

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BLAST of CmaCh20G002010 vs. ExPASy Swiss-Prot
Match: Q9M0H6 (Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 1.8e-184
Identity = 294/417 (70.50%), Postives = 355/417 (85.13%), Query Frame = 0


Query: 10  ILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWNSYWFMDHAV 69
           ILG   C AFIY+SF D  L      K+GFV+RNGT+F+V+ +  Y+NGWNSYWFMDHAV
Sbjct: 12  ILGFFICAAFIYLSFRDLWLNHKGKAKLGFVKRNGTQFVVDDKPLYVNGWNSYWFMDHAV 71

Query: 70  EGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQ 129
           + H +  V  ML A  KMGLTVCRTWAF+DGGYNALQ+ PGRF+E+VF+ALDHVIAE+R+
Sbjct: 72  DEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEARK 131

Query: 130 HGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTR 189
           H +RLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFF+DPSIR YFKNY+K +LTR
Sbjct: 132 HDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLTR 191

Query: 190 RNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLE 249
           +NS+TGIEYRNDPTIF WELINEPRC TD SG TLQ+WI+EM+ +IKSID KHLLTVGLE
Sbjct: 192 KNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGLE 251

Query: 250 GFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELQFV 309
           GFYGPNSPK  T NPE+WAS+LG+DF++NS   +IDFASVHIYPDHWFH+Q FE++L+FV
Sbjct: 252 GFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFV 311

Query: 310 SKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYESAKRKRSGA 369
            KWM SHIEDG KELKKPV+FTEFGLS+ NK + P+QRD+FY+ ++D++Y+SAKRK+SGA
Sbjct: 312 VKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSGA 371

Query: 370 GSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLKALKNMC 427
           G+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSCRL ++ G +++ K LK +C
Sbjct: 372 GTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQK-LKELC 427

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BLAST of CmaCh20G002010 vs. ExPASy Swiss-Prot
Match: Q0JJD4 (Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 542.0 bits (1395), Expect = 6.2e-153
Identity = 259/441 (58.73%), Postives = 326/441 (73.92%), Query Frame = 0


Query: 3   GNGL-FYPILGLACCLAFIYISFGDWKLGD-------------TKAPKMGFVERNGTKFM 62
           GNGL  Y ILGLA C+A +Y S G+  L D               A  + FVER G +  
Sbjct: 4   GNGLILYHILGLASCIALVYFSLGEVDLRDALPSLPFSGGASRAAAASLPFVERRGKRLF 63

Query: 63  VNGRAFYINGWNSYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVF 122
           ++GR FYINGWNSYW MD AVE + +PRV +M R A  MGLTVCRTWAF+DG YNALQ+ 
Sbjct: 64  LDGRPFYINGWNSYWLMDLAVEPNTRPRVSSMFRTAVSMGLTVCRTWAFNDGSYNALQLS 123

Query: 123 PGRFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDS 182
           PG F+E+VFKALD V+AE+ +HG+RL+LSL NNL AYGGK QYV+WAW++GVGL++SNDS
Sbjct: 124 PGHFDERVFKALDRVVAEASEHGVRLILSLANNLDAYGGKRQYVRWAWEEGVGLTASNDS 183

Query: 183 FFYDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWI 242
           FF+DP+IR YFK Y+KT+L R+N +TG+EYR+DPTI  WEL+NEPRC +D SGDTLQ W+
Sbjct: 184 FFFDPAIRDYFKVYLKTLLMRKNHLTGLEYRDDPTILAWELMNEPRCTSDPSGDTLQRWM 243

Query: 243 EEMSAYIKSIDKKHLLTVGLEGFYGP-NSPKKSTANPEEW-ASRLGSDFIRNSEIPHIDF 302
           EEMSAY+KSIDKKHLLTVG EGFYGP +S +K   NP EW  +  G+DFIRNS+I  IDF
Sbjct: 244 EEMSAYVKSIDKKHLLTVGTEGFYGPTSSQEKLNINPGEWFPNNYGADFIRNSKIQDIDF 303

Query: 303 ASVHIYPDHWFHDQDFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQ 362
           ASVH+YPD+W      +++L+F+++W+ +H+EDGD EL+KPV+ TEFGLS   +GF  A 
Sbjct: 304 ASVHVYPDNWLQHASLDEKLKFMTRWITAHVEDGDGELEKPVLVTEFGLSHQVEGFEDAH 363

Query: 363 RDRFYKTVYDIIYESAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQS 422
           RD  Y+ VYDI++ SA+R  +  G+L WQ   EGMEE +D F IVP ER S+ RLI EQS
Sbjct: 364 RDVLYRAVYDIVHGSARRGGAAGGALVWQLAAEGMEEYHDGFSIVPSERPSMMRLIKEQS 423

Query: 423 CRLARIG-GDIQQLKALKNMC 427
           CRLA +  G+    K LK +C
Sbjct: 424 CRLAAVRYGEEGARKVLKTVC 444

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BLAST of CmaCh20G002010 vs. ExPASy Swiss-Prot
Match: Q0DCM5 (Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN6 PE=2 SV=2)

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-115
Identity = 210/428 (49.07%), Postives = 283/428 (66.12%), Query Frame = 0


Query: 8   YPILGLACCLAFIYISF------------GDW-KLG-DTKAPKMGFVERNGTKFM--VNG 67
           Y +LGL   LA +Y+++            G W KL      P+M F  R GT F+    G
Sbjct: 8   YSLLGLLLLLAVVYLTWFPTTHDGGGGGGGGWVKLPVPWLQPRMPFAARRGTHFVDADTG 67

Query: 68  RAFYINGWNSYWFMDHAVEGHRKPRVRA-MLRAARKMGLTVCRTWAFSDGGYNALQVFPG 127
              Y+NGWNSYW +       R P + A MLR  R+MGL+VCRTWAFSDGG  ALQ+ PG
Sbjct: 68  SPLYVNGWNSYWLLP-----ARSPALAAEMLRRGRRMGLSVCRTWAFSDGGPGALQISPG 127

Query: 128 RFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFF 187
           RF+E VF+ LD+VI E+R++ IRL+L LVNNL   GGK QYV+WA   G  +++S DSF+
Sbjct: 128 RFSEAVFQVLDYVIYEARRNHIRLILCLVNNLDNLGGKAQYVQWAQAAGANMTNSTDSFY 187

Query: 188 YDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEE 247
             P+I+ Y+K+Y+K +LTRRNS + I Y ++P IF WEL+NEPRC++++SG  LQ WI E
Sbjct: 188 SHPTIKRYYKDYVKAILTRRNSYSRIRYSDEPAIFAWELMNEPRCVSNSSGPYLQAWIAE 247

Query: 248 MSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVH 307
           M+AY+KS+D  HL+TVG EGFYGP   ++   NP EWA+ L SDFI+NS + HIDFASVH
Sbjct: 248 MAAYVKSLDTNHLVTVGTEGFYGPGIAERLGVNPGEWAASLCSDFIQNSAVEHIDFASVH 307

Query: 308 IYPDHWFHDQDFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGL---SDLNKGFTPAQR 367
            YPD W      E++++++S W+ SH+ D ++ LKKPV+FTE G    SD N   T   R
Sbjct: 308 AYPDSWLPRASLEEKVRYLSNWVDSHLNDSEQILKKPVLFTEVGYLQHSDANSNST-VDR 367

Query: 368 DRFYKTVYDIIYESAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSC 416
           D   + VYD IY+SA++ ++G+G+L WQ +VEG     D+F +V  +R S Y LI  QSC
Sbjct: 368 DIILRIVYDKIYDSARKLQAGSGALIWQLMVEGTHMYGDNFSVVARDRPSTYSLITNQSC 427

[Close]

BLAST of CmaCh20G002010 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 7.2e-109
Identity = 184/393 (46.82%), Postives = 256/393 (65.14%), Query Frame = 0


Query: 20  IYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWNSYWFMDHAVEGHRKPRVRA 79
           I I    W+LG    P  GFV   G +F +NG  +Y NG+N+YW M  A +  ++ ++  
Sbjct: 12  IVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKIST 71

Query: 80  MLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQHGIRLLLSLV 139
             + A + GLTV RTWAFSDGGY ALQ  PG +NE +F+ LD  +AE+R+HGI+++LS  
Sbjct: 72  AFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFA 131

Query: 140 NNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRRNSITGIEYR 199
           NN +++GG+ QYV WA   G  +SS +D FF D  ++ ++KN+IK VL R N+ T + Y+
Sbjct: 132 NNYESFGGRKQYVDWARSRGRPVSSEDD-FFTDSLVKDFYKNHIKAVLNRFNTFTKVHYK 191

Query: 200 NDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKK 259
           +DPTI  WEL+NEPRC +D SG  +Q WI EM+A++KS+D+ HLL  GLEGFYG +SP+ 
Sbjct: 192 DDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQS 251

Query: 260 STANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELQFVSKWMLSHIED 319
            T NP     + G+DFI N+ IP IDF +VH YPD WF D   + ++ F++KW+ +HI+D
Sbjct: 252 KTLNP---PGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQD 311

Query: 320 GDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYESAKRKRSGAGSLAWQFLVE 379
               L KP++  EFG S    G+TPAQRD  + TVY  IY SAKR  + AG L WQ LV 
Sbjct: 312 AQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVN 371

Query: 380 GMEESNDDFGIVPWERSSIYRLIIEQSCRLARI 413
           G++   D +GI+  + SS   +I +QS +L  I
Sbjct: 372 GIDNFQDGYGIILSQSSSTVNVISQQSRKLTLI 400

[Close]

BLAST of CmaCh20G002010 vs. ExPASy TrEMBL
Match: A0A6J1JF54 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111483854 PE=3 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.3e-257
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360
           DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE
Sbjct: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNMC 427
           ALKNMC
Sbjct: 421 ALKNMC 426

[Close]

BLAST of CmaCh20G002010 vs. ExPASy TrEMBL
Match: A0A6J1FXY1 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 5.5e-253
Identity = 417/426 (97.89%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKL DTKAPKMGFVERNGT+FMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEG+RKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360
           DFE+EL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYD+IY+
Sbjct: 301 DFEEELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNMC 427
           ALKN+C
Sbjct: 421 ALKNVC 426

[Close]

BLAST of CmaCh20G002010 vs. ExPASy TrEMBL
Match: A0A5D3C3G0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001850 PE=3 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.1e-237
Identity = 392/427 (91.80%), Postives = 413/427 (96.72%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKA-PKMGFVERNGTKFMVNGRAFYINGW 60
           MAGNGLFYPILG A CLAFIY+SFGD ++GDTK  P + FVERNGT+FM+NG+AFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKA 120
           NSYWFMDH+VE +RKPR+RAML+AARKMGLTVCRTWAF+DGGYNALQV PGRFNE+VFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240
           KNYIKTVLTR+NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPH+DFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIY 360
           QDFEDEL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRD FYKTVYD+IY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 ESAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQL 420
           +SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY+LIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKNMC 427
           KALK +C
Sbjct: 421 KALKYVC 427

[Close]

BLAST of CmaCh20G002010 vs. ExPASy TrEMBL
Match: A0A1S3BJC2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.1e-237
Identity = 392/427 (91.80%), Postives = 413/427 (96.72%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKA-PKMGFVERNGTKFMVNGRAFYINGW 60
           MAGNGLFYPILG A CLAFIY+SFGD ++GDTK  P + FVERNGT+FM+NG+AFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKA 120
           NSYWFMDH+VE +RKPR+RAML+AARKMGLTVCRTWAF+DGGYNALQV PGRFNE+VFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240
           KNYIKTVLTR+NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPH+DFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIY 360
           QDFEDEL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRD FYKTVYD+IY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 ESAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQL 420
           +SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY+LIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKNMC 427
           KALK +C
Sbjct: 421 KALKYVC 427

[Close]

BLAST of CmaCh20G002010 vs. ExPASy TrEMBL
Match: A0A0A0KB35 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 5.5e-237
Identity = 390/427 (91.33%), Postives = 412/427 (96.49%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKA-PKMGFVERNGTKFMVNGRAFYINGW 60
           MAGNGLFYPILG A CLAFIY+SFGD ++GDTK  P + FVERNGT+FM+NG+AFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKA 120
           NSYWFMDH+VE +RKPR+RAML+AARKMGLTVCRTWAF+DGGYNALQV PGRFNE+VFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240
           KNY+KTVLTR+NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEI H+DFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIY 360
           QDFEDEL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRD FYKTVYD+IY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIY 360

Query: 361 ESAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQL 420
           +SAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY+LIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKNMC 427
           KALK +C
Sbjct: 421 KALKYVC 427

[Close]

BLAST of CmaCh20G002010 vs. NCBI nr
Match: XP_022985973.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 898.3 bits (2320), Expect = 2.6e-257
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360
           DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE
Sbjct: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNMC 427
           ALKNMC
Sbjct: 421 ALKNMC 426

[Close]

BLAST of CmaCh20G002010 vs. NCBI nr
Match: XP_023512794.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887.9 bits (2293), Expect = 3.5e-254
Identity = 419/426 (98.36%), Postives = 425/426 (99.77%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGT+FMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEG+RKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360
           DFEDEL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYD+IY+
Sbjct: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNMC 427
           ALKN+C
Sbjct: 421 ALKNVC 426

[Close]

BLAST of CmaCh20G002010 vs. NCBI nr
Match: XP_022944323.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 882.9 bits (2280), Expect = 1.1e-252
Identity = 417/426 (97.89%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKL DTKAPKMGFVERNGT+FMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEG+RKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360
           DFE+EL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYD+IY+
Sbjct: 301 DFEEELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNMC 427
           ALKN+C
Sbjct: 421 ALKNVC 426

[Close]

BLAST of CmaCh20G002010 vs. NCBI nr
Match: KAG6570514.1 (Mannan endo-1,4-beta-mannosidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 882.9 bits (2280), Expect = 1.1e-252
Identity = 417/426 (97.89%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKL DTKAPKMGFVERNGT+FMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEG+RKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360
           DFEDEL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYD+IY+
Sbjct: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY+LIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNMC 427
           ALKN+C
Sbjct: 421 ALKNVC 426

[Close]

BLAST of CmaCh20G002010 vs. NCBI nr
Match: KAG7010376.1 (Mannan endo-1,4-beta-mannosidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 882.5 bits (2279), Expect = 1.5e-252
Identity = 417/426 (97.89%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKL DTKAPKMGFVERNGT+FMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEG+RKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360
           DFEDEL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYD+IY+
Sbjct: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI+RLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNMC 427
           ALKN+C
Sbjct: 421 ALKNVC 426

[Close]

BLAST of CmaCh20G002010 vs. TAIR 10
Match: AT2G20680.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 691.8 bits (1784), Expect = 3.4e-199
Identity = 314/412 (76.21%), Postives = 363/412 (88.11%), Query Frame = 0


Query: 3   GNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWNSY 62
           GNG   PILG   C+AFIY+SFGD   G     ++ FV+RNGT+F+V+G+A Y+NGWNSY
Sbjct: 6   GNGPVIPILGFLTCVAFIYLSFGDLWFGLKTEGELAFVKRNGTQFVVDGKALYVNGWNSY 65

Query: 63  WFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDH 122
           WFMDHAV  H + RV AML A  KMGLTVCRTWAF+DGGYNALQ+ PGRF+E+VFKALDH
Sbjct: 66  WFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFKALDH 125

Query: 123 VIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNY 182
           VIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQ+GVGLSSSNDSFF+DPSIR YFKNY
Sbjct: 126 VIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSNDSFFFDPSIRRYFKNY 185

Query: 183 IKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKH 242
           +  +LTR+NS+TGIEYRNDPTIF WELINEPRCM+D SGDTLQ+WI EM+A+IKSID KH
Sbjct: 186 LTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDWINEMTAFIKSIDNKH 245

Query: 243 LLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDF 302
           LLTVGLEGFYGP+SPKK T NPE WAS LGSDF+RNS+ P+IDFASVHIYPDHWFHDQ F
Sbjct: 246 LLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVHIYPDHWFHDQGF 305

Query: 303 EDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYESA 362
           E++L+FV KWMLSHIEDGDKELKKPV+FTEFGLS+LNK + P+QRDRFY+T++D+IY+SA
Sbjct: 306 EEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKSA 365

Query: 363 KRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGG 415
           KRKRSGAG+L WQFL+EGME  NDDFGIVPWE+ SI RL+IEQSCRL+RI G
Sbjct: 366 KRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITG 417

[Close]

BLAST of CmaCh20G002010 vs. TAIR 10
Match: AT4G28320.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 646.7 bits (1667), Expect = 1.3e-185
Identity = 294/417 (70.50%), Postives = 355/417 (85.13%), Query Frame = 0


Query: 10  ILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWNSYWFMDHAV 69
           ILG   C AFIY+SF D  L      K+GFV+RNGT+F+V+ +  Y+NGWNSYWFMDHAV
Sbjct: 12  ILGFFICAAFIYLSFRDLWLNHKGKAKLGFVKRNGTQFVVDDKPLYVNGWNSYWFMDHAV 71

Query: 70  EGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQ 129
           + H +  V  ML A  KMGLTVCRTWAF+DGGYNALQ+ PGRF+E+VF+ALDHVIAE+R+
Sbjct: 72  DEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEARK 131

Query: 130 HGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTR 189
           H +RLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFF+DPSIR YFKNY+K +LTR
Sbjct: 132 HDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLTR 191

Query: 190 RNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLE 249
           +NS+TGIEYRNDPTIF WELINEPRC TD SG TLQ+WI+EM+ +IKSID KHLLTVGLE
Sbjct: 192 KNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGLE 251

Query: 250 GFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELQFV 309
           GFYGPNSPK  T NPE+WAS+LG+DF++NS   +IDFASVHIYPDHWFH+Q FE++L+FV
Sbjct: 252 GFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFV 311

Query: 310 SKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYESAKRKRSGA 369
            KWM SHIEDG KELKKPV+FTEFGLS+ NK + P+QRD+FY+ ++D++Y+SAKRK+SGA
Sbjct: 312 VKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSGA 371

Query: 370 GSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLKALKNMC 427
           G+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSCRL ++ G +++ K LK +C
Sbjct: 372 GTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQK-LKELC 427

[Close]

BLAST of CmaCh20G002010 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 5.1e-110
Identity = 184/393 (46.82%), Postives = 256/393 (65.14%), Query Frame = 0


Query: 20  IYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWNSYWFMDHAVEGHRKPRVRA 79
           I I    W+LG    P  GFV   G +F +NG  +Y NG+N+YW M  A +  ++ ++  
Sbjct: 12  IVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKIST 71

Query: 80  MLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQHGIRLLLSLV 139
             + A + GLTV RTWAFSDGGY ALQ  PG +NE +F+ LD  +AE+R+HGI+++LS  
Sbjct: 72  AFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFA 131

Query: 140 NNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRRNSITGIEYR 199
           NN +++GG+ QYV WA   G  +SS +D FF D  ++ ++KN+IK VL R N+ T + Y+
Sbjct: 132 NNYESFGGRKQYVDWARSRGRPVSSEDD-FFTDSLVKDFYKNHIKAVLNRFNTFTKVHYK 191

Query: 200 NDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKK 259
           +DPTI  WEL+NEPRC +D SG  +Q WI EM+A++KS+D+ HLL  GLEGFYG +SP+ 
Sbjct: 192 DDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQS 251

Query: 260 STANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELQFVSKWMLSHIED 319
            T NP     + G+DFI N+ IP IDF +VH YPD WF D   + ++ F++KW+ +HI+D
Sbjct: 252 KTLNP---PGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQD 311

Query: 320 GDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYESAKRKRSGAGSLAWQFLVE 379
               L KP++  EFG S    G+TPAQRD  + TVY  IY SAKR  + AG L WQ LV 
Sbjct: 312 AQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVN 371

Query: 380 GMEESNDDFGIVPWERSSIYRLIIEQSCRLARI 413
           G++   D +GI+  + SS   +I +QS +L  I
Sbjct: 372 GIDNFQDGYGIILSQSSSTVNVISQQSRKLTLI 400

[Close]

BLAST of CmaCh20G002010 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 385.2 bits (988), Expect = 6.9e-107
Identity = 176/371 (47.44%), Postives = 257/371 (69.27%), Query Frame = 0


Query: 40  VERNGTKFMVNGRAFYINGWNSYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSD 99
           V+R G +F +NG+ FY+NG+N+YW M  A +   + +V  + + A  +G+TV RTWAF+D
Sbjct: 48  VQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFND 107

Query: 100 GGYNALQVFPGRFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDG 159
           G + ALQ  P  ++E+VFKALD V++E+R++ IRL+LSLVNN  AYGGK QYVKW    G
Sbjct: 108 GQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASG 167

Query: 160 VGLSSSNDSFFYDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDA 219
           + L +S+D FF +P++R +++++++TVL R N+ T I Y+NDPTIF WEL+NEPRC +D 
Sbjct: 168 LNL-TSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDP 227

Query: 220 SGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNS 279
           SGD LQ WI+EM+ ++KS+D KHL+ +GLEGFYGP++P ++  NP  +A+++G+DFIRN+
Sbjct: 228 SGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNN 287

Query: 280 EIPHIDFASVHIYPDHWFHDQDFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLN 339
           ++  IDFASVH+YPD W         L+F S WM +H+ED +  L  PV+FTEFG+S  +
Sbjct: 288 QVLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHD 347

Query: 340 KGFTPAQRDRFYKTVYDIIYESAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY 399
            GF  + RD    TVY +   S ++  +GAGSL WQ   +G E  +D + +      +  
Sbjct: 348 PGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTAS 407

Query: 400 RLIIEQSCRLA 411
           ++I  QS RLA
Sbjct: 408 KIISLQSKRLA 417

[Close]

BLAST of CmaCh20G002010 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 365.2 bits (936), Expect = 7.4e-101
Identity = 173/404 (42.82%), Postives = 253/404 (62.62%), Query Frame = 0


Query: 16  CLAF-----IYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWNSYWFMDHAVE 75
           CL F     I I+     LG   A   GFV R G +F++NG+ FY NG+N+YW    A +
Sbjct: 3   CLCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEATD 62

Query: 76  GHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQH 135
              + ++  + + A    LT+ RTW F DGGY ALQ+ PG ++E+ F+ LD  IAE+++ 
Sbjct: 63  STTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAKRL 122

Query: 136 GIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRR 195
           GI+++++ VNN   +GG+ QYV WA   G  + SS+D F+ +P ++ Y+KN++KT++ R 
Sbjct: 123 GIKMIITFVNNYSDFGGRKQYVDWAKNTGQNV-SSDDDFYTNPLVKQYYKNHVKTMVNRV 182

Query: 196 NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEG 255
           N+ T +EY+++PTI GWEL+NEP+C  D SG TL  W+ EM+ Y+KS+D KHLL+ GLEG
Sbjct: 183 NTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLEG 242

Query: 256 FYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELQFVS 315
           FYG +SP++ T+     A+ LG+DFI N ++  IDFAS+H YPD WF + D +  L  + 
Sbjct: 243 FYGDSSPQRKTSLNPVAANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLLR 302

Query: 316 KWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYESAKRKRSGAG 375
           KW+  H+ED    LKKP++  EFG      G+T AQRD  +   +D IYESA++    AG
Sbjct: 303 KWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQAQRDAVFNATFDTIYESAEKGGPAAG 362

Query: 376 SLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGG 415
           +L W  + +GM    D   IV  E S+   +I E+S +L  I G
Sbjct: 363 ALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLIRG 405

The following BLAST results are available for this feature:

BLAST of CmaCh20G002010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7Y223	4.8e-198	76.21	Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 ...	[more]
Q9M0H6	1.8e-184	70.50	Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 ...	[more]
Q0JJD4	6.2e-153	58.73	Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q0DCM5	1.3e-115	49.07	Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9FJZ3	7.2e-109	46.82	Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 ...	[more]

BLAST of CmaCh20G002010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JF54	1.3e-257	100.00	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111483854 PE=...	[more]
A0A6J1FXY1	5.5e-253	97.89	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 P...	[more]
A0A5D3C3G0	1.1e-237	91.80	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BJC2	1.1e-237	91.80	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV...	[more]
A0A0A0KB35	5.5e-237	91.33	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3...	[more]

BLAST of CmaCh20G002010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022985973.1	2.6e-257	100.00	mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita maxima]	[more]
XP_023512794.1	3.5e-254	98.36	mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022944323.1	1.1e-252	97.89	mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita moschata]	[more]
KAG6570514.1	1.1e-252	97.89	Mannan endo-1,4-beta-mannosidase 2, partial [Cucurbita argyrosperma subsp. soror...	[more]
KAG7010376.1	1.5e-252	97.89	Mannan endo-1,4-beta-mannosidase 2, partial [Cucurbita argyrosperma subsp. argyr...	[more]

BLAST of CmaCh20G002010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G20680.1	3.4e-199	76.21	Glycosyl hydrolase superfamily protein	[more]
AT4G28320.1	1.3e-185	70.50	Glycosyl hydrolase superfamily protein	[more]
AT5G66460.1	5.1e-110	46.82	Glycosyl hydrolase superfamily protein	[more]
AT5G01930.1	6.9e-107	47.44	Glycosyl hydrolase superfamily protein	[more]
AT3G10890.1	7.4e-101	42.82	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 56..350 e-value: 3.9E-15 score: 56.0
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 35..413 e-value: 5.1E-134 score: 448.9
None	No IPR available	PANTHER	PTHR31451:SF61	BNAA09G43620D PROTEIN	coord: 5..426
IPR045053	Mannan endo-1,4-beta-mannosidase-like	PANTHER	PTHR31451	FAMILY NOT NAMED	coord: 5..426
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 38..398

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G002010.1	CmaCh20G002010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds