CmaCh17G005660 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G005660
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionLipase_3 domain-containing protein
LocationCma_Chr17: 4616995 .. 4620024 (+)
RNA-Seq ExpressionCmaCh17G005660
SyntenyCmaCh17G005660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGGTCTAATAAACGCAAATAGTTTCATTGATCCATCAAGTGAGAGTTCTTCAAGAGTCATTATGGCTTGTACTTCAGTGGCTATTGGCAATTCTTCAGCCACTTCAATGACAAAAGACATCATCTTCAAAGAGTATAATGGGATTCGTCGTTCACACTCGAGCAAGGATATTCGAGAACGAACTTGCATCAAAAGATCTTATTCTGATAACCATATTTGTTACTCTGCAAACAAAATTCATGCTACTTCAACACAGCCAAAGCCAAAAAACAACAACAATAATAATAACTCTATGGGAATGAGGATTTTTCCTTTGAAATTTTCAGGGTCTTTTCTTCCTCATCCAATCAGATCCTTTTTGTTTGACATGGAGGAGACCTCGAAGGATCTGAGTAGTGAAGAGGAGGTGACAAGGAGAGCGAATTGGATTGAGAGATTGTTGGAGATTCGAAGGCATTGGCGGACGAAGCAGAAAAAAGGAGTTGAGAATGATATTTATACACATCATGATGAAACTGGTGAGAGATTTTGTGGTGAAGATGATGGTGGGTGTGAAGTGGATTATTATGACTCTGAGGATGAAGGAGAGTTTAGTTTTGATACTGAATCATTTGCAAGATTTTTAGTTAAGGTTCCATTGTCTGATACCAAGGTGTTTTCTCAGCTCGCATTCCTGTCGAACATGGCCTATGTTATACCAGAAATAAAGGTACTCATTTGATTCAGAAAGGATCACACATTATTTTCTAAGAAAACGAGGTCATGAACATGCAGAGTCGGGGATTATAGTATGCCTAAAAAGCGGCATACTCATTTAATTTATATGATTAGAGTCTGACTGGTGTCTATTGTGCTGAATAAGTGTTCGAGTATTGGAGTGTCGGGCATATGTCGAACATGGACATATTTATTTGTGATGTCTTAGTCTTAGATTGCGATGTCTGTACTAAAAAGAAAGATTATAGTGACATGTGAATTTTGATATCCATTGAATTTGATTTATTTAATTACAGGCAGAGGAATTAGAACGATGCTGTGGCCTGAAATTCGTTACGTCCTCGTTGCGCAAGAAAGCGAATGGTGCAGCCTTTAATATGAAAGAAAAACAGGATCAGCATTCAACCTGTATATCTGATGAGGCCTTAAATGTTGGTGAAGTTTGTTCAGGGGAAGCCTTGCAGTTGGAGAGGACACAACAAATCCCCACGACTGTTGCTTATGAGATAGCTGCAACAGCTGCATCGTACATGCACTCGAGAGCCAAGAATCCTTCATCCCATCCTTTGGAGCCACAAGAGAGAGGAGAGAGTTCGTGTCGGGCATATAATCCCGAGGTTGCTGCTTATGTTGCAGCTTCAACAATGACTGCTGTGGTTGCTGCTGAGGAGGTGCAGAAACAGGAGACTGCAAAGGGCCTCCAATCTCTTCACTCTTCTCCTTGTGAATGGTTTGTTTGTGACGACATCCACAGCTCGACTCGATACTTCATCATTCAGGTGTTTACTCCAATCCTTCTAACTAAAATATTTTTTGAAGTATAGCAACTAAATAGATAAAAGAACGAATGCTTGAGAACAAATAGGAACTTTTTAAAGAAAAGTGGGATTAATAGGCTCTACTTATAATTTAACCAGTTAGATTGTGTGATATACAGGGATCGGATTCGTTGGCATCTTGGCAAGCAAATCTTTTTTTCGAGCCCACGAAGTTCGAGGTAAAATAATAGCTTGAATGGCCTCTATTACTTGGCAAGTCAGAAAAAAGAAAAGTACATGTAGTTGTAATTAAAAATGGACTTAATGTAGGGAAGTGATGTGCTTGTTCATAGAGGAATATATGAAGCTGCAAAGGGAATATACAAGCAATTCATACCAGAAATTATAGAGCATTTGAAAACATATGGAACCCATGCAAAGTTTCAGTTTACTGGTCATTCTCTTGGAGGCAGTCTTTCCCTTTTGGTGCACTTGATGCTACTGAAGAATGAGATTGTGAAGCCTTGTATGCTTCAACCAGTGGTCACTTTTGGTTCTCCATTTGTATTCTGTGGTGGACACAAGATTCTAATTGAATTGGGTTTGGATGAAGACGATATCCGTTGCGTCATAATGCACCGGGATATCGTTCCGAGGGCCTTCTCCTGCAACTACCCAAACCATGTCGCTGCTGTTCTGAAGCGCTTGAGTGGATCGTTTCGGTCACACTCTTGCCTCAACAAAAGTGTAAGCGATCTTAGGATATGCTTTCTGTTGAAGTTGATCGAGTTTTTGGTTAAAGTATACAAAATATTGCTCATATGTATTGTGAGATCCCACATTGGTTGGGGAGGAGAACGAAACACCCTCATAAAGGTGTGGAAACCTCTCCCTAGTAGACGCATTTTAACAACCTTGAGGGTAAGAAGTTTAAAAGAAAAAGTCCAAAGAAGACAATATCTGCCGGTGGTGGCTTTGGGCATTTACAGTTTTGAACTTTATGTTTATTATGCAGAAATTACTATATTCTCCAATGGGTAAGATATTCATATTGCAACCTGAAGAGATGTCATCTCCCCCTCATCCTATGCTACCTGAGGGAAGTGCCCTCTATACTTTGGACAGCACCCAAAATGGATACTCCACAGGGATCCTTAGGGCGTTCCTGAATTGCCCTCATCCACTTGAGACACTCAGTGACCCTACAGCTTATGGGTCAGAGGGTACAATTTTAAGGGACCATGACTCGAGTTTCTATCTGAAGGCTTTGAATGGGGTTCTTAACCAACACACGAAGATGCATACCGGCAAAGTCAGAAAGCAGAGGAAATTGTTATTGCCATTACTTACCTCGCCATCTCCAGAGTTATGGAGGCATGATGGAAATTTGGACAATAAGAATAGTTTGGTATCCAATGAGATAATGACAGGTGTTTAACACCATTTTCCATGAGCTTTGTAATCATCTTGCAAGTCACCTCTGTAAATGCATAAACGTACTTGAACTGGTTTGGTTTAACTTTAAATAAATAGGTTGGGACACTGGAGTAGG

mRNA sequence

AAAGGGTCTAATAAACGCAAATAGTTTCATTGATCCATCAAGTGAGAGTTCTTCAAGAGTCATTATGGCTTGTACTTCAGTGGCTATTGGCAATTCTTCAGCCACTTCAATGACAAAAGACATCATCTTCAAAGAGTATAATGGGATTCGTCGTTCACACTCGAGCAAGGATATTCGAGAACGAACTTGCATCAAAAGATCTTATTCTGATAACCATATTTGTTACTCTGCAAACAAAATTCATGCTACTTCAACACAGCCAAAGCCAAAAAACAACAACAATAATAATAACTCTATGGGAATGAGGATTTTTCCTTTGAAATTTTCAGGGTCTTTTCTTCCTCATCCAATCAGATCCTTTTTGTTTGACATGGAGGAGACCTCGAAGGATCTGAGTAGTGAAGAGGAGGTGACAAGGAGAGCGAATTGGATTGAGAGATTGTTGGAGATTCGAAGGCATTGGCGGACGAAGCAGAAAAAAGGAGTTGAGAATGATATTTATACACATCATGATGAAACTGGTGAGAGATTTTGTGGTGAAGATGATGGTGGGTGTGAAGTGGATTATTATGACTCTGAGGATGAAGGAGAGTTTAGTTTTGATACTGAATCATTTGCAAGATTTTTAGTTAAGGTTCCATTGTCTGATACCAAGGTGTTTTCTCAGCTCGCATTCCTGTCGAACATGGCCTATGTTATACCAGAAATAAAGGCAGAGGAATTAGAACGATGCTGTGGCCTGAAATTCGTTACGTCCTCGTTGCGCAAGAAAGCGAATGGTGCAGCCTTTAATATGAAAGAAAAACAGGATCAGCATTCAACCTGTATATCTGATGAGGCCTTAAATGTTGGTGAAGTTTGTTCAGGGGAAGCCTTGCAGTTGGAGAGGACACAACAAATCCCCACGACTGTTGCTTATGAGATAGCTGCAACAGCTGCATCGTACATGCACTCGAGAGCCAAGAATCCTTCATCCCATCCTTTGGAGCCACAAGAGAGAGGAGAGAGTTCGTGTCGGGCATATAATCCCGAGGTTGCTGCTTATGTTGCAGCTTCAACAATGACTGCTGTGGTTGCTGCTGAGGAGGTGCAGAAACAGGAGACTGCAAAGGGCCTCCAATCTCTTCACTCTTCTCCTTGTGAATGGTTTGTTTGTGACGACATCCACAGCTCGACTCGATACTTCATCATTCAGGGATCGGATTCGTTGGCATCTTGGCAAGCAAATCTTTTTTTCGAGCCCACGAAGTTCGAGGGAAGTGATGTGCTTGTTCATAGAGGAATATATGAAGCTGCAAAGGGAATATACAAGCAATTCATACCAGAAATTATAGAGCATTTGAAAACATATGGAACCCATGCAAAGTTTCAGTTTACTGGTCATTCTCTTGGAGGCAGTCTTTCCCTTTTGGTGCACTTGATGCTACTGAAGAATGAGATTGTGAAGCCTTGTATGCTTCAACCAGTGGTCACTTTTGGTTCTCCATTTGTATTCTGTGGTGGACACAAGATTCTAATTGAATTGGGTTTGGATGAAGACGATATCCGTTGCGTCATAATGCACCGGGATATCGTTCCGAGGGCCTTCTCCTGCAACTACCCAAACCATGTCGCTGCTGTTCTGAAGCGCTTGAGTGGATCGTTTCGGTCACACTCTTGCCTCAACAAAAGTAAATTACTATATTCTCCAATGGGTAAGATATTCATATTGCAACCTGAAGAGATGTCATCTCCCCCTCATCCTATGCTACCTGAGGGAAGTGCCCTCTATACTTTGGACAGCACCCAAAATGGATACTCCACAGGGATCCTTAGGGCGTTCCTGAATTGCCCTCATCCACTTGAGACACTCAGTGACCCTACAGCTTATGGGTCAGAGGGTACAATTTTAAGGGACCATGACTCGAGTTTCTATCTGAAGGCTTTGAATGGGGTTCTTAACCAACACACGAAGATGCATACCGGCAAAGTCAGAAAGCAGAGGAAATTGTTATTGCCATTACTTACCTCGCCATCTCCAGAGTTATGGAGGCATGATGGAAATTTGGACAATAAGAATAGTTTGGTATCCAATGAGATAATGACAGGTGTTTAACACCATTTTCCATGAGCTTTGTAATCATCTTGCAAGTCACCTCTGTAAATGCATAAACGTACTTGAACTGGTTTGGTTTAACTTTAAATAAATAGGTTGGGACACTGGAGTAGG

Coding sequence (CDS)

ATGGCTTGTACTTCAGTGGCTATTGGCAATTCTTCAGCCACTTCAATGACAAAAGACATCATCTTCAAAGAGTATAATGGGATTCGTCGTTCACACTCGAGCAAGGATATTCGAGAACGAACTTGCATCAAAAGATCTTATTCTGATAACCATATTTGTTACTCTGCAAACAAAATTCATGCTACTTCAACACAGCCAAAGCCAAAAAACAACAACAATAATAATAACTCTATGGGAATGAGGATTTTTCCTTTGAAATTTTCAGGGTCTTTTCTTCCTCATCCAATCAGATCCTTTTTGTTTGACATGGAGGAGACCTCGAAGGATCTGAGTAGTGAAGAGGAGGTGACAAGGAGAGCGAATTGGATTGAGAGATTGTTGGAGATTCGAAGGCATTGGCGGACGAAGCAGAAAAAAGGAGTTGAGAATGATATTTATACACATCATGATGAAACTGGTGAGAGATTTTGTGGTGAAGATGATGGTGGGTGTGAAGTGGATTATTATGACTCTGAGGATGAAGGAGAGTTTAGTTTTGATACTGAATCATTTGCAAGATTTTTAGTTAAGGTTCCATTGTCTGATACCAAGGTGTTTTCTCAGCTCGCATTCCTGTCGAACATGGCCTATGTTATACCAGAAATAAAGGCAGAGGAATTAGAACGATGCTGTGGCCTGAAATTCGTTACGTCCTCGTTGCGCAAGAAAGCGAATGGTGCAGCCTTTAATATGAAAGAAAAACAGGATCAGCATTCAACCTGTATATCTGATGAGGCCTTAAATGTTGGTGAAGTTTGTTCAGGGGAAGCCTTGCAGTTGGAGAGGACACAACAAATCCCCACGACTGTTGCTTATGAGATAGCTGCAACAGCTGCATCGTACATGCACTCGAGAGCCAAGAATCCTTCATCCCATCCTTTGGAGCCACAAGAGAGAGGAGAGAGTTCGTGTCGGGCATATAATCCCGAGGTTGCTGCTTATGTTGCAGCTTCAACAATGACTGCTGTGGTTGCTGCTGAGGAGGTGCAGAAACAGGAGACTGCAAAGGGCCTCCAATCTCTTCACTCTTCTCCTTGTGAATGGTTTGTTTGTGACGACATCCACAGCTCGACTCGATACTTCATCATTCAGGGATCGGATTCGTTGGCATCTTGGCAAGCAAATCTTTTTTTCGAGCCCACGAAGTTCGAGGGAAGTGATGTGCTTGTTCATAGAGGAATATATGAAGCTGCAAAGGGAATATACAAGCAATTCATACCAGAAATTATAGAGCATTTGAAAACATATGGAACCCATGCAAAGTTTCAGTTTACTGGTCATTCTCTTGGAGGCAGTCTTTCCCTTTTGGTGCACTTGATGCTACTGAAGAATGAGATTGTGAAGCCTTGTATGCTTCAACCAGTGGTCACTTTTGGTTCTCCATTTGTATTCTGTGGTGGACACAAGATTCTAATTGAATTGGGTTTGGATGAAGACGATATCCGTTGCGTCATAATGCACCGGGATATCGTTCCGAGGGCCTTCTCCTGCAACTACCCAAACCATGTCGCTGCTGTTCTGAAGCGCTTGAGTGGATCGTTTCGGTCACACTCTTGCCTCAACAAAAGTAAATTACTATATTCTCCAATGGGTAAGATATTCATATTGCAACCTGAAGAGATGTCATCTCCCCCTCATCCTATGCTACCTGAGGGAAGTGCCCTCTATACTTTGGACAGCACCCAAAATGGATACTCCACAGGGATCCTTAGGGCGTTCCTGAATTGCCCTCATCCACTTGAGACACTCAGTGACCCTACAGCTTATGGGTCAGAGGGTACAATTTTAAGGGACCATGACTCGAGTTTCTATCTGAAGGCTTTGAATGGGGTTCTTAACCAACACACGAAGATGCATACCGGCAAAGTCAGAAAGCAGAGGAAATTGTTATTGCCATTACTTACCTCGCCATCTCCAGAGTTATGGAGGCATGATGGAAATTTGGACAATAAGAATAGTTTGGTATCCAATGAGATAATGACAGGTGTTTAA

Protein sequence

MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIHATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRANWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFDTESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGAAFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAKNPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCEWFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIPEIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGGHKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYSPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGNLDNKNSLVSNEIMTGV
Homology
BLAST of CmaCh17G005660 vs. ExPASy Swiss-Prot
Match: Q7Y220 (Phospholipase A1 PLIP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP1 PE=1 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 1.7e-176
Identity = 360/693 (51.95%), Postives = 451/693 (65.08%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKD-IRERTCIKRSYSDNHICYSA--- 60
           MA TS A  N          + +E+ G+RRS S +D + +   I+RS SDNH+C  +   
Sbjct: 7   MASTSPAAAND---------VLREHIGLRRSLSGQDLVLKGGGIRRSSSDNHLCCRSGNN 66

Query: 61  -NKIHATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEE 120
            N+I A S +P  K       S  + +F  + S S +P PI++ LF+  +TS+D    +E
Sbjct: 67  NNRILAVSVRPGMK------TSRSVGVFSFQISSSIIPSPIKTLLFE-TDTSQDEQESDE 126

Query: 121 V----------TRRANWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCG-EDDGGC 180
           +           ++ANW+ERLLEIRR W+ +QK    N      +E+ +  CG E++ GC
Sbjct: 127 IEIETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDVA--EESVDVTCGCEEEEGC 186

Query: 181 EVDYYDSEDEGEFSFDTESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCC 240
             +Y     +    +  ESF+R LVKV  S+ K  SQLA+L N+AY IPEIK E+L R  
Sbjct: 187 IANYGSVNGD----WGRESFSRLLVKVSWSEAKKLSQLAYLCNLAYTIPEIKGEDLRRNY 246

Query: 241 GLKFVTSSLRKKANGAAFNMKEKQD-QHSTCISDEALNVGEVCSGEALQLERTQQIPTTV 300
           GLKFVTSSL KKA  A    K +QD  H   I+   L        E+ +  +     +  
Sbjct: 247 GLKFVTSSLEKKAKAAILREKLEQDPTHVPVITSPDL--------ESEKQSQRSASSSAS 306

Query: 301 AYEIAATAASYMHSRAKNPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQ 360
           AY+IAA+AASY+HS  +   S P+            Y    AA  AASTMTAVVAA E +
Sbjct: 307 AYKIAASAASYIHSCKEYDLSEPI------------YKSAAAAQAAASTMTAVVAAGEEE 366

Query: 361 KQETAKGLQSLHSSPCEWFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLV 420
           K E A+ LQSL SSPCEWFVCDD ++ TR F+IQGSDSLASW+ANLFFEPTKFE +DVLV
Sbjct: 367 KLEAARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANLFFEPTKFEDTDVLV 426

Query: 421 HRGIYEAAKGIYKQFIPEIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPC 480
           HRGIYEAAKGIY+QF+PEI EHL  +G  AKFQFTGHSLGGSLSL+V+LML+   +V   
Sbjct: 427 HRGIYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLIVNLMLISRGLVSSE 486

Query: 481 MLQPVVTFGSPFVFCGGHKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRL 540
            ++ VVTFGSPFVFCGG KIL ELGLDE  + CV+MHRDIVPRAFSCNYP+HVA VLKRL
Sbjct: 487 AMKSVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFSCNYPDHVALVLKRL 546

Query: 541 SGSFRSHSCLNKSKLLYSPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGIL 600
           +GSFR+H CLNK+KLLYSPMGK++ILQP E  SP HP LP G+ALY L+++  GYS   L
Sbjct: 547 NGSFRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALYILENSNEGYSPTAL 606

Query: 601 RAFLNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLL 660
           RAFLN PHPLETLS   AYGSEG++LRDHDS  Y+KA+NGVL QHTK+   K R QR+ +
Sbjct: 607 RAFLNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNGVLRQHTKLIVRKARIQRRSV 649

Query: 661 LPLLTSPSPELWRHDGNLDNKNSLVSNEIMTGV 677
            P+LTS    L        N++   + EIMT V
Sbjct: 667 WPVLTSAGRGL--------NESLTTAEEIMTRV 649

BLAST of CmaCh17G005660 vs. ExPASy Swiss-Prot
Match: F4HXL0 (Phospholipase A1 PLIP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP2 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.0e-125
Identity = 256/568 (45.07%), Postives = 362/568 (63.73%), Query Frame = 0

Query: 104 EETSKDLSSEEEVTRRANWIERLLEIRRHWRTKQKKG---VENDIYTHHDET--GERFCG 163
           EET+ ++ +E    R  +W+ ++L+++  W+ ++++    VE++     +E    +    
Sbjct: 92  EETAVEMDTER---RNGSWVLKILDVQSTWKHEEEEDDDEVEDEDGDEDEEVELDDAVVS 151

Query: 164 EDDGGCEVDYY---DSEDEGEFSFDTESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEI 223
           EDDGGC+V      D  +  +F  D ESF++ L +V L ++K+++QL++L N+AY I +I
Sbjct: 152 EDDGGCDVCSVLEDDGNEANKFQLDRESFSKLLRRVTLPESKLYAQLSYLGNLAYSISKI 211

Query: 224 KAEELERCCGLKFVTSSLRKKANGAAFNMKEKQDQHSTCISDEALNVGEVCSGEALQLER 283
           K   L +  GL+FVTSS  K  +       E   +    +  E     EV   E  +  +
Sbjct: 212 KPANLSKYYGLRFVTSSAEKTESALKAENGEVSGETKPIVEAEE----EV---EEEEKNK 271

Query: 284 TQQIPTTVAYEIAATAASYMHSRAKN--PSSHPLEPQERGESSCRAYNPEVAAYVAASTM 343
           +++I  + AYEI A+AASY+HSR  N  P +   + +   +      N E ++ VA S +
Sbjct: 272 SRKISASAAYEIVASAASYLHSRTNNILPFNSSSKAENSDKHDVNLTNAESSSDVAYS-V 331

Query: 344 TAVVAAEEVQKQETAKGLQSLHSSPCEWFVCDDIHSSTRYFIIQGSDSLASWQANLFFEP 403
           T+VVAAEE  KQ  A  L+S  SSPC+WF+CDD  S TR+ +IQGS+SLASWQANL FEP
Sbjct: 332 TSVVAAEEDVKQAVADDLKSTISSPCDWFICDDDQSHTRFVVIQGSESLASWQANLLFEP 391

Query: 404 TKFEGSDVLVHRGIYEAAKGIYKQFIPEIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLM 463
            +FEG   +VHRGIYEAAKG+Y+Q +PE+  H+KT+GT AKF+FTGHSLGGSLSLL++LM
Sbjct: 392 IEFEGLGAIVHRGIYEAAKGMYEQMLPEVKAHIKTHGTSAKFRFTGHSLGGSLSLLLNLM 451

Query: 464 LLKNEIVKPCMLQPVVTFGSPFVFCGGHKILIELGLDEDDIRCVIMHRDIVPRAFSCNYP 523
           LL    V    L PV+T+G+PFV CGG ++L +LGL +  ++ ++MHRDIVPRAFSCNYP
Sbjct: 452 LLVRGEVPASSLLPVITYGAPFVLCGGDRLLKKLGLPKSHVQAIVMHRDIVPRAFSCNYP 511

Query: 524 NHVAAVLKRLSGSFRSHSCLNKSKLLYSPMGKIFILQPEEMSSPPHPMLPEGSALYTLDS 583
            HVA +LK ++G+FRSH CLNK  +LYSPMG++ ILQP+E  SP H +LP G+ LY L  
Sbjct: 512 YHVAELLKAVNGNFRSHPCLNKQSMLYSPMGELLILQPDETFSPGHELLPSGNGLYLL-- 571

Query: 584 TQNGYSTGI-------LRA----FLNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALN 643
           T +  S  I       LRA    FLN PHPL+ LSD +AYGS GTI RDHD + YLKA+ 
Sbjct: 572 TSDFESPDIEDSDEERLRAAQTVFLNTPHPLDILSDRSAYGSSGTIQRDHDMNSYLKAVR 631

Query: 644 GVLNQH-TKMHTGKVRKQRKLLLPLLTS 650
            V+ +   ++   K   +R L  P+L +
Sbjct: 632 SVIRKEVNQIRRAKREHRRSLWWPILVA 646

BLAST of CmaCh17G005660 vs. ExPASy Swiss-Prot
Match: Q940L4 (Phospholipase A1 PLIP3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP3 PE=1 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 5.1e-117
Identity = 251/522 (48.08%), Postives = 321/522 (61.49%), Query Frame = 0

Query: 121 NWIERLLEIRRHWRTKQKK-----GVENDIYTH--HDETGERFCGEDDGGCEVDYYDSED 180
           NW+ ++LE+   W+ K+++     G E D        +  E  C E D  C +D  D ++
Sbjct: 90  NWVLKILEVGSIWKGKRQRSGGGGGGEEDEEEEVAEPKKKEDLCEECD-FCRIDDDDEDE 149

Query: 181 EGEFSFDTESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSL 240
           E E +     F+  L K+P+ D ++F++L+FL N+AY IP+IK E L +   L+FVTSS+
Sbjct: 150 EKEKT--VFEFSEMLSKIPVEDAQMFAKLSFLGNLAYSIPKIKPENLLKYQKLRFVTSSI 209

Query: 241 RKKANGAAFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAAS 300
            K+       M  K ++++    DE               E+ + I   VAY IAA+AAS
Sbjct: 210 EKR-------MSLKVEENNNGEEDE---------------EKKKLINPAVAYRIAASAAS 269

Query: 301 YMHSRAKNPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQS 360
            + S +K  S  P        SS R  N E +    A ++TAVVAA+E  KQ  A  L+S
Sbjct: 270 RLFSHSK--SVLPF------GSSKRQDNEEASLLATADSVTAVVAAKEEVKQAVADDLKS 329

Query: 361 LHSSPCEWFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKG 420
             S PCEWFVCDD  S TR+F IQGSDSLASWQANL FEP  FE  DVLVHRGIYEAAKG
Sbjct: 330 NRSPPCEWFVCDDDKSGTRFFFIQGSDSLASWQANLLFEPVPFEDLDVLVHRGIYEAAKG 389

Query: 421 IYKQFIPEIIEHLKTYGTHAKF-QFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFG 480
           IY+Q +PE+  HL + G +  F +F+GHSLGGSLSLLV+LMLL    V    L PV+TFG
Sbjct: 390 IYEQMLPEVHAHLNSRGKNRAFLRFSGHSLGGSLSLLVNLMLLIRGQVPASSLLPVITFG 449

Query: 481 SPFVFCGGHKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSC 540
           SP + CGG ++L +LGL +  +  + MHRDIVPRAFSCNYPN  A +LK L+G+FR+H C
Sbjct: 450 SPCIMCGGDRLLQKLGLPKSHLLGISMHRDIVPRAFSCNYPNRAAKLLKALNGNFRNHPC 509

Query: 541 LNKSKLLYSPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRA----FLN 600
           LN   +LYSPMGK+ ILQP E  SPPHP+LP GS LY L S     +   LRA    F N
Sbjct: 510 LNNQNVLYSPMGKLLILQPSERFSPPHPLLPPGSGLYLLASKNTDETEKSLRAAKILFFN 569

Query: 601 CPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLNQHTK 631
            PHPLE LSD  +YGSEG I R+HD S YLKAL  V+ +  K
Sbjct: 570 SPHPLEILSDRRSYGSEGKIKRNHDMSSYLKALRHVIRKELK 578

BLAST of CmaCh17G005660 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 9.9e-04
Identity = 43/139 (30.94%), Postives = 66/139 (47.48%), Query Frame = 0

Query: 380 DSLASWQANLFFEPTKFEGSDV-----LVHRGIYEAAKGIYKQFIPEIIEHLKTYGTHAK 439
           D   ++  NLF + +  E +       +V R  Y A + I K+ + E          +A+
Sbjct: 281 DDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSE--------HENAR 340

Query: 440 FQFTGHSLGGSLSLLVHLMLLKN---EIVKPCMLQPVVTFGSPFVFCGGHKILI----EL 499
           F  TGHSLGG+L++L   +L+ N   EI+K   L  V TFG P +  G  ++ +    +L
Sbjct: 341 FVVTGHSLGGALAILFPTLLVLNEETEIMK--RLLGVYTFGQPRI--GNREVGLFMKAQL 400

Query: 500 GLDEDDIRCVIMHRDIVPR 507
               D    V+   DIVPR
Sbjct: 401 NQPVDRYFRVVYCNDIVPR 407

BLAST of CmaCh17G005660 vs. ExPASy TrEMBL
Match: A0A6J1JSU1 (uncharacterized protein LOC111488580 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488580 PE=4 SV=1)

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 676/676 (100.00%), Postives = 676/676 (100.00%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD
Sbjct: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA
Sbjct: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK
Sbjct: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT
Sbjct: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660

Query: 661 LDNKNSLVSNEIMTGV 677
           LDNKNSLVSNEIMTGV
Sbjct: 661 LDNKNSLVSNEIMTGV 676

BLAST of CmaCh17G005660 vs. ExPASy TrEMBL
Match: A0A6J1JUY6 (uncharacterized protein LOC111488580 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488580 PE=4 SV=1)

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 676/676 (100.00%), Postives = 676/676 (100.00%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 94  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD
Sbjct: 154 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 273

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK
Sbjct: 274 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 334 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 394 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT
Sbjct: 574 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 693

Query: 661 LDNKNSLVSNEIMTGV 677
           LDNKNSLVSNEIMTGV
Sbjct: 694 LDNKNSLVSNEIMTGV 709

BLAST of CmaCh17G005660 vs. ExPASy TrEMBL
Match: A0A6J1EP51 (uncharacterized protein LOC111436294 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436294 PE=4 SV=1)

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 658/676 (97.34%), Postives = 665/676 (98.37%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 94  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWRTKQKKGVENDIY HHDETGERFCGE+DGGCEVDYYDSEDEGEFSFD
Sbjct: 154 NWIERLLEIRRHWRTKQKKGVENDIYAHHDETGERFCGEEDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSL KKANGA
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLLKKANGA 273

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           A NMKEKQDQHSTCISDEALNV  V SGEALQLERTQQIPTTVAYEIAATAASYMHSRAK
Sbjct: 274 AINMKEKQDQHSTCISDEALNVAGVGSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQE+GE + RAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 334 NPSSHPLEPQEKGEGTSRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 394 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLK YGT AKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKKYGTQAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILIELGLDEDDIRC+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILIELGLDEDDIRCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SP+GKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYS G+LRAFLNCPHPLETLSDPT
Sbjct: 574 SPLGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSIGLLRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVL+QHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLDQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 693

Query: 661 LDNKNSLVSNEIMTGV 677
           LDNKNSLVSNEIMTGV
Sbjct: 694 LDNKNSLVSNEIMTGV 709

BLAST of CmaCh17G005660 vs. ExPASy TrEMBL
Match: A0A6J1ENN9 (uncharacterized protein LOC111436294 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436294 PE=4 SV=1)

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 658/676 (97.34%), Postives = 665/676 (98.37%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWRTKQKKGVENDIY HHDETGERFCGE+DGGCEVDYYDSEDEGEFSFD
Sbjct: 121 NWIERLLEIRRHWRTKQKKGVENDIYAHHDETGERFCGEEDGGCEVDYYDSEDEGEFSFD 180

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSL KKANGA
Sbjct: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLLKKANGA 240

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           A NMKEKQDQHSTCISDEALNV  V SGEALQLERTQQIPTTVAYEIAATAASYMHSRAK
Sbjct: 241 AINMKEKQDQHSTCISDEALNVAGVGSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQE+GE + RAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 301 NPSSHPLEPQEKGEGTSRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLK YGT AKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 421 EIIEHLKKYGTQAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILIELGLDEDDIRC+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILIELGLDEDDIRCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SP+GKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYS G+LRAFLNCPHPLETLSDPT
Sbjct: 541 SPLGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSIGLLRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVL+QHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLDQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660

Query: 661 LDNKNSLVSNEIMTGV 677
           LDNKNSLVSNEIMTGV
Sbjct: 661 LDNKNSLVSNEIMTGV 676

BLAST of CmaCh17G005660 vs. ExPASy TrEMBL
Match: A0A0A0K4E8 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G325710 PE=4 SV=1)

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 574/677 (84.79%), Postives = 614/677 (90.69%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNS A SMTK+IIFKEY+ +RRSHSSKD+RER CIKRSYSDN ICYSANKIH
Sbjct: 38  MACTSVAIGNSPAASMTKEIIFKEYSMLRRSHSSKDVRERACIKRSYSDNQICYSANKIH 97

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPK  N NNNS GM IFPLKFSGSFLP+ +RSFLFDMEETSK+L++EEEVT+RA
Sbjct: 98  ATSTQPKPK--NKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTTEEEVTKRA 157

Query: 121 NWIERLLEIRRHWRTKQKK-GVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSF 180
           NWIERLLEIR  WR KQ+K G+END+Y  HDE+ E  CG DDGGCEVDYYDSEDE   +F
Sbjct: 158 NWIERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTF 217

Query: 181 DTESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANG 240
           DTESFAR+L++VP+SDTKVFSQLAFLSNMAYVIP IKAE+LE   GL+FVTSSL KKA  
Sbjct: 218 DTESFARYLIRVPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLQFVTSSLHKKAEA 277

Query: 241 AAFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRA 300
           AA N+KEK DQHSTCISDEA NVGE CS EAL+LERTQ+IPTTVAYEIAATAASY+HSR 
Sbjct: 278 AAINIKEKLDQHSTCISDEAFNVGESCSVEALKLERTQEIPTTVAYEIAATAASYVHSRV 337

Query: 301 KNPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPC 360
           KN SSHPLE QE+G+ S R YNPEVAAYVAASTMTAVVAAEEVQKQE AK LQSLHSSPC
Sbjct: 338 KNTSSHPLESQEKGDGSTRVYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPC 397

Query: 361 EWFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFI 420
           EWFVCDD  + TR FIIQGSDSLASWQANLFFEPTKFEG+DVLVHRGIYEAAKGIYKQF+
Sbjct: 398 EWFVCDDTDTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFM 457

Query: 421 PEIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCG 480
           PEII+HLK YG HA+FQFTGHSLGGSLSLLVHLMLLKN IVKP MLQPVVTFGSPFVFCG
Sbjct: 458 PEIIDHLKKYGAHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCG 517

Query: 481 GHKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLL 540
           GHKIL ELGLDED+I C+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLL
Sbjct: 518 GHKILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLL 577

Query: 541 YSPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDP 600
           YSP+GK+FILQP+EMSSPPHPMLP+GSALYTLDSTQNGYS G+LRAFLNCPHPLETLSDP
Sbjct: 578 YSPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDP 637

Query: 601 TAYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDG 660
           TAYGSEGTILRDHDSSFYLKALNGVL QHTKM  GKVR QRKLL PLL SPSPELW HDG
Sbjct: 638 TAYGSEGTILRDHDSSFYLKALNGVLKQHTKMAVGKVRNQRKLLWPLLASPSPELWSHDG 697

Query: 661 NLDNKNSLVSNEIMTGV 677
           NLDN NSL+SNEIMTGV
Sbjct: 698 NLDNNNSLLSNEIMTGV 712

BLAST of CmaCh17G005660 vs. NCBI nr
Match: XP_022992170.1 (uncharacterized protein LOC111488580 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 676/676 (100.00%), Postives = 676/676 (100.00%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 94  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD
Sbjct: 154 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 273

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK
Sbjct: 274 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 334 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 394 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT
Sbjct: 574 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 693

Query: 661 LDNKNSLVSNEIMTGV 677
           LDNKNSLVSNEIMTGV
Sbjct: 694 LDNKNSLVSNEIMTGV 709

BLAST of CmaCh17G005660 vs. NCBI nr
Match: XP_022992171.1 (uncharacterized protein LOC111488580 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 676/676 (100.00%), Postives = 676/676 (100.00%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD
Sbjct: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA
Sbjct: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK
Sbjct: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT
Sbjct: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660

Query: 661 LDNKNSLVSNEIMTGV 677
           LDNKNSLVSNEIMTGV
Sbjct: 661 LDNKNSLVSNEIMTGV 676

BLAST of CmaCh17G005660 vs. NCBI nr
Match: XP_023547682.1 (uncharacterized protein LOC111806547 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1330.9 bits (3443), Expect = 0.0e+00
Identity = 661/676 (97.78%), Postives = 664/676 (98.22%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 94  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWRTKQKKGVENDIY HHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD
Sbjct: 154 NWIERLLEIRRHWRTKQKKGVENDIYAHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELER CGLKFVTSSL KKANGA
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERSCGLKFVTSSLLKKANGA 273

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           A NMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPT VAYEIAATAASYMHSRAK
Sbjct: 274 AINMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTNVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQE+GE SCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 334 NPSSHPLEPQEKGEGSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHS TRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 394 WFVCDDIHSPTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLK YGT AKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKKYGTQAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKIL ELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILNELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SP+GKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYS G+LRAFLNCPHPLETLSDPT
Sbjct: 574 SPLGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSIGLLRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHT KVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTSKVRKQRKLLLPLLTSPSPELWRHDGN 693

Query: 661 LDNKNSLVSNEIMTGV 677
           LDNKNSLVSNEIMTGV
Sbjct: 694 LDNKNSLVSNEIMTGV 709

BLAST of CmaCh17G005660 vs. NCBI nr
Match: XP_023547683.1 (uncharacterized protein LOC111806547 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1330.9 bits (3443), Expect = 0.0e+00
Identity = 661/676 (97.78%), Postives = 664/676 (98.22%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWRTKQKKGVENDIY HHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD
Sbjct: 121 NWIERLLEIRRHWRTKQKKGVENDIYAHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELER CGLKFVTSSL KKANGA
Sbjct: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERSCGLKFVTSSLLKKANGA 240

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           A NMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPT VAYEIAATAASYMHSRAK
Sbjct: 241 AINMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTNVAYEIAATAASYMHSRAK 300

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQE+GE SCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 301 NPSSHPLEPQEKGEGSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHS TRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 361 WFVCDDIHSPTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLK YGT AKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 421 EIIEHLKKYGTQAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKIL ELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILNELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SP+GKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYS G+LRAFLNCPHPLETLSDPT
Sbjct: 541 SPLGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSIGLLRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHT KVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTSKVRKQRKLLLPLLTSPSPELWRHDGN 660

Query: 661 LDNKNSLVSNEIMTGV 677
           LDNKNSLVSNEIMTGV
Sbjct: 661 LDNKNSLVSNEIMTGV 676

BLAST of CmaCh17G005660 vs. NCBI nr
Match: KAG6575321.1 (Phospholipase A1 PLIP1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1327.4 bits (3434), Expect = 0.0e+00
Identity = 658/676 (97.34%), Postives = 664/676 (98.22%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60
           MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120
           ATSTQPKPKNNNNNNNS+GMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA
Sbjct: 94  ATSTQPKPKNNNNNNNSLGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180
           NWIERLLEIRRHWR KQKKGVENDIY HHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD
Sbjct: 154 NWIERLLEIRRHWRAKQKKGVENDIYAHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240
           TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELE+CC LKFVTSSL KKANGA
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELEQCCSLKFVTSSLLKKANGA 273

Query: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300
           A NMKEKQDQHSTCISDEAL VGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK
Sbjct: 274 AINMKEKQDQHSTCISDEALIVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360
           NPSSHPLEPQ++GE SCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE
Sbjct: 334 NPSSHPLEPQDKGEGSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420
           WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP
Sbjct: 394 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480
           EIIEHLK YGT AKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKKYGTQAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKIL ELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILNELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600
           SP+GKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYS G+LRAFLNCPHPLETLSDPT
Sbjct: 574 SPLGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSIGLLRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 693

Query: 661 LDNKNSLVSNEIMTGV 677
           LDN NSLVSNEIMTGV
Sbjct: 694 LDNNNSLVSNEIMTGV 709

BLAST of CmaCh17G005660 vs. TAIR 10
Match: AT3G61680.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 620.9 bits (1600), Expect = 1.2e-177
Identity = 360/693 (51.95%), Postives = 451/693 (65.08%), Query Frame = 0

Query: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKD-IRERTCIKRSYSDNHICYSA--- 60
           MA TS A  N          + +E+ G+RRS S +D + +   I+RS SDNH+C  +   
Sbjct: 7   MASTSPAAAND---------VLREHIGLRRSLSGQDLVLKGGGIRRSSSDNHLCCRSGNN 66

Query: 61  -NKIHATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEE 120
            N+I A S +P  K       S  + +F  + S S +P PI++ LF+  +TS+D    +E
Sbjct: 67  NNRILAVSVRPGMK------TSRSVGVFSFQISSSIIPSPIKTLLFE-TDTSQDEQESDE 126

Query: 121 V----------TRRANWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCG-EDDGGC 180
           +           ++ANW+ERLLEIRR W+ +QK    N      +E+ +  CG E++ GC
Sbjct: 127 IEIETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDVA--EESVDVTCGCEEEEGC 186

Query: 181 EVDYYDSEDEGEFSFDTESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCC 240
             +Y     +    +  ESF+R LVKV  S+ K  SQLA+L N+AY IPEIK E+L R  
Sbjct: 187 IANYGSVNGD----WGRESFSRLLVKVSWSEAKKLSQLAYLCNLAYTIPEIKGEDLRRNY 246

Query: 241 GLKFVTSSLRKKANGAAFNMKEKQD-QHSTCISDEALNVGEVCSGEALQLERTQQIPTTV 300
           GLKFVTSSL KKA  A    K +QD  H   I+   L        E+ +  +     +  
Sbjct: 247 GLKFVTSSLEKKAKAAILREKLEQDPTHVPVITSPDL--------ESEKQSQRSASSSAS 306

Query: 301 AYEIAATAASYMHSRAKNPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQ 360
           AY+IAA+AASY+HS  +   S P+            Y    AA  AASTMTAVVAA E +
Sbjct: 307 AYKIAASAASYIHSCKEYDLSEPI------------YKSAAAAQAAASTMTAVVAAGEEE 366

Query: 361 KQETAKGLQSLHSSPCEWFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLV 420
           K E A+ LQSL SSPCEWFVCDD ++ TR F+IQGSDSLASW+ANLFFEPTKFE +DVLV
Sbjct: 367 KLEAARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANLFFEPTKFEDTDVLV 426

Query: 421 HRGIYEAAKGIYKQFIPEIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPC 480
           HRGIYEAAKGIY+QF+PEI EHL  +G  AKFQFTGHSLGGSLSL+V+LML+   +V   
Sbjct: 427 HRGIYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLIVNLMLISRGLVSSE 486

Query: 481 MLQPVVTFGSPFVFCGGHKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRL 540
            ++ VVTFGSPFVFCGG KIL ELGLDE  + CV+MHRDIVPRAFSCNYP+HVA VLKRL
Sbjct: 487 AMKSVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFSCNYPDHVALVLKRL 546

Query: 541 SGSFRSHSCLNKSKLLYSPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGIL 600
           +GSFR+H CLNK+KLLYSPMGK++ILQP E  SP HP LP G+ALY L+++  GYS   L
Sbjct: 547 NGSFRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALYILENSNEGYSPTAL 606

Query: 601 RAFLNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLL 660
           RAFLN PHPLETLS   AYGSEG++LRDHDS  Y+KA+NGVL QHTK+   K R QR+ +
Sbjct: 607 RAFLNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNGVLRQHTKLIVRKARIQRRSV 649

Query: 661 LPLLTSPSPELWRHDGNLDNKNSLVSNEIMTGV 677
            P+LTS    L        N++   + EIMT V
Sbjct: 667 WPVLTSAGRGL--------NESLTTAEEIMTRV 649

BLAST of CmaCh17G005660 vs. TAIR 10
Match: AT1G02660.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 452.2 bits (1162), Expect = 7.3e-127
Identity = 256/568 (45.07%), Postives = 362/568 (63.73%), Query Frame = 0

Query: 104 EETSKDLSSEEEVTRRANWIERLLEIRRHWRTKQKKG---VENDIYTHHDET--GERFCG 163
           EET+ ++ +E    R  +W+ ++L+++  W+ ++++    VE++     +E    +    
Sbjct: 92  EETAVEMDTER---RNGSWVLKILDVQSTWKHEEEEDDDEVEDEDGDEDEEVELDDAVVS 151

Query: 164 EDDGGCEVDYY---DSEDEGEFSFDTESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEI 223
           EDDGGC+V      D  +  +F  D ESF++ L +V L ++K+++QL++L N+AY I +I
Sbjct: 152 EDDGGCDVCSVLEDDGNEANKFQLDRESFSKLLRRVTLPESKLYAQLSYLGNLAYSISKI 211

Query: 224 KAEELERCCGLKFVTSSLRKKANGAAFNMKEKQDQHSTCISDEALNVGEVCSGEALQLER 283
           K   L +  GL+FVTSS  K  +       E   +    +  E     EV   E  +  +
Sbjct: 212 KPANLSKYYGLRFVTSSAEKTESALKAENGEVSGETKPIVEAEE----EV---EEEEKNK 271

Query: 284 TQQIPTTVAYEIAATAASYMHSRAKN--PSSHPLEPQERGESSCRAYNPEVAAYVAASTM 343
           +++I  + AYEI A+AASY+HSR  N  P +   + +   +      N E ++ VA S +
Sbjct: 272 SRKISASAAYEIVASAASYLHSRTNNILPFNSSSKAENSDKHDVNLTNAESSSDVAYS-V 331

Query: 344 TAVVAAEEVQKQETAKGLQSLHSSPCEWFVCDDIHSSTRYFIIQGSDSLASWQANLFFEP 403
           T+VVAAEE  KQ  A  L+S  SSPC+WF+CDD  S TR+ +IQGS+SLASWQANL FEP
Sbjct: 332 TSVVAAEEDVKQAVADDLKSTISSPCDWFICDDDQSHTRFVVIQGSESLASWQANLLFEP 391

Query: 404 TKFEGSDVLVHRGIYEAAKGIYKQFIPEIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLM 463
            +FEG   +VHRGIYEAAKG+Y+Q +PE+  H+KT+GT AKF+FTGHSLGGSLSLL++LM
Sbjct: 392 IEFEGLGAIVHRGIYEAAKGMYEQMLPEVKAHIKTHGTSAKFRFTGHSLGGSLSLLLNLM 451

Query: 464 LLKNEIVKPCMLQPVVTFGSPFVFCGGHKILIELGLDEDDIRCVIMHRDIVPRAFSCNYP 523
           LL    V    L PV+T+G+PFV CGG ++L +LGL +  ++ ++MHRDIVPRAFSCNYP
Sbjct: 452 LLVRGEVPASSLLPVITYGAPFVLCGGDRLLKKLGLPKSHVQAIVMHRDIVPRAFSCNYP 511

Query: 524 NHVAAVLKRLSGSFRSHSCLNKSKLLYSPMGKIFILQPEEMSSPPHPMLPEGSALYTLDS 583
            HVA +LK ++G+FRSH CLNK  +LYSPMG++ ILQP+E  SP H +LP G+ LY L  
Sbjct: 512 YHVAELLKAVNGNFRSHPCLNKQSMLYSPMGELLILQPDETFSPGHELLPSGNGLYLL-- 571

Query: 584 TQNGYSTGI-------LRA----FLNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALN 643
           T +  S  I       LRA    FLN PHPL+ LSD +AYGS GTI RDHD + YLKA+ 
Sbjct: 572 TSDFESPDIEDSDEERLRAAQTVFLNTPHPLDILSDRSAYGSSGTIQRDHDMNSYLKAVR 631

Query: 644 GVLNQH-TKMHTGKVRKQRKLLLPLLTS 650
            V+ +   ++   K   +R L  P+L +
Sbjct: 632 SVIRKEVNQIRRAKREHRRSLWWPILVA 646

BLAST of CmaCh17G005660 vs. TAIR 10
Match: AT3G62590.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 423.3 bits (1087), Expect = 3.6e-118
Identity = 251/522 (48.08%), Postives = 321/522 (61.49%), Query Frame = 0

Query: 121 NWIERLLEIRRHWRTKQKK-----GVENDIYTH--HDETGERFCGEDDGGCEVDYYDSED 180
           NW+ ++LE+   W+ K+++     G E D        +  E  C E D  C +D  D ++
Sbjct: 90  NWVLKILEVGSIWKGKRQRSGGGGGGEEDEEEEVAEPKKKEDLCEECD-FCRIDDDDEDE 149

Query: 181 EGEFSFDTESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSL 240
           E E +     F+  L K+P+ D ++F++L+FL N+AY IP+IK E L +   L+FVTSS+
Sbjct: 150 EKEKT--VFEFSEMLSKIPVEDAQMFAKLSFLGNLAYSIPKIKPENLLKYQKLRFVTSSI 209

Query: 241 RKKANGAAFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAAS 300
            K+       M  K ++++    DE               E+ + I   VAY IAA+AAS
Sbjct: 210 EKR-------MSLKVEENNNGEEDE---------------EKKKLINPAVAYRIAASAAS 269

Query: 301 YMHSRAKNPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQS 360
            + S +K  S  P        SS R  N E +    A ++TAVVAA+E  KQ  A  L+S
Sbjct: 270 RLFSHSK--SVLPF------GSSKRQDNEEASLLATADSVTAVVAAKEEVKQAVADDLKS 329

Query: 361 LHSSPCEWFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKG 420
             S PCEWFVCDD  S TR+F IQGSDSLASWQANL FEP  FE  DVLVHRGIYEAAKG
Sbjct: 330 NRSPPCEWFVCDDDKSGTRFFFIQGSDSLASWQANLLFEPVPFEDLDVLVHRGIYEAAKG 389

Query: 421 IYKQFIPEIIEHLKTYGTHAKF-QFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFG 480
           IY+Q +PE+  HL + G +  F +F+GHSLGGSLSLLV+LMLL    V    L PV+TFG
Sbjct: 390 IYEQMLPEVHAHLNSRGKNRAFLRFSGHSLGGSLSLLVNLMLLIRGQVPASSLLPVITFG 449

Query: 481 SPFVFCGGHKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSC 540
           SP + CGG ++L +LGL +  +  + MHRDIVPRAFSCNYPN  A +LK L+G+FR+H C
Sbjct: 450 SPCIMCGGDRLLQKLGLPKSHLLGISMHRDIVPRAFSCNYPNRAAKLLKALNGNFRNHPC 509

Query: 541 LNKSKLLYSPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRA----FLN 600
           LN   +LYSPMGK+ ILQP E  SPPHP+LP GS LY L S     +   LRA    F N
Sbjct: 510 LNNQNVLYSPMGKLLILQPSERFSPPHPLLPPGSGLYLLASKNTDETEKSLRAAKILFFN 569

Query: 601 CPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLNQHTK 631
            PHPLE LSD  +YGSEG I R+HD S YLKAL  V+ +  K
Sbjct: 570 SPHPLEILSDRRSYGSEGKIKRNHDMSSYLKALRHVIRKELK 578

BLAST of CmaCh17G005660 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 7.1e-05
Identity = 43/139 (30.94%), Postives = 66/139 (47.48%), Query Frame = 0

Query: 380 DSLASWQANLFFEPTKFEGSDV-----LVHRGIYEAAKGIYKQFIPEIIEHLKTYGTHAK 439
           D   ++  NLF + +  E +       +V R  Y A + I K+ + E          +A+
Sbjct: 281 DDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSE--------HENAR 340

Query: 440 FQFTGHSLGGSLSLLVHLMLLKN---EIVKPCMLQPVVTFGSPFVFCGGHKILI----EL 499
           F  TGHSLGG+L++L   +L+ N   EI+K   L  V TFG P +  G  ++ +    +L
Sbjct: 341 FVVTGHSLGGALAILFPTLLVLNEETEIMK--RLLGVYTFGQPRI--GNREVGLFMKAQL 400

Query: 500 GLDEDDIRCVIMHRDIVPR 507
               D    V+   DIVPR
Sbjct: 401 NQPVDRYFRVVYCNDIVPR 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7Y2201.7e-17651.95Phospholipase A1 PLIP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP1 P... [more]
F4HXL01.0e-12545.07Phospholipase A1 PLIP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP2 P... [more]
Q940L45.1e-11748.08Phospholipase A1 PLIP3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP3 P... [more]
F4JFU89.9e-0430.94Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JSU10.0e+00100.00uncharacterized protein LOC111488580 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JUY60.0e+00100.00uncharacterized protein LOC111488580 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EP510.0e+0097.34uncharacterized protein LOC111436294 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1ENN90.0e+0097.34uncharacterized protein LOC111436294 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0K4E80.0e+0084.79Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G325710 PE... [more]
Match NameE-valueIdentityDescription
XP_022992170.10.0e+00100.00uncharacterized protein LOC111488580 isoform X1 [Cucurbita maxima][more]
XP_022992171.10.0e+00100.00uncharacterized protein LOC111488580 isoform X2 [Cucurbita maxima][more]
XP_023547682.10.0e+0097.78uncharacterized protein LOC111806547 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023547683.10.0e+0097.78uncharacterized protein LOC111806547 isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG6575321.10.0e+0097.34Phospholipase A1 PLIP1, chloroplastic, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
AT3G61680.11.2e-17751.95alpha/beta-Hydrolases superfamily protein [more]
AT1G02660.17.3e-12745.07alpha/beta-Hydrolases superfamily protein [more]
AT3G62590.13.6e-11848.08alpha/beta-Hydrolases superfamily protein [more]
AT3G14360.17.1e-0530.94alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 374..511
e-value: 1.8E-23
score: 83.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 318..541
e-value: 1.0E-26
score: 95.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 357..549
NoneNo IPR availablePANTHERPTHR46483:SF1PHOSPHOLIPASE A1 PLIP1, CHLOROPLASTICcoord: 1..676
NoneNo IPR availableCDDcd00519Lipase_3coord: 357..513
e-value: 9.62663E-25
score: 101.399
IPR043367Phospholipase A1 PLIP1/2/3, chloroplasticPANTHERPTHR46483PHOSPHOLIPASE A1 PLIP2, CHLOROPLASTICcoord: 1..676

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G005660.1CmaCh17G005660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015908 fatty acid transport
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0009534 chloroplast thylakoid
molecular_function GO:0052739 phosphatidylserine 1-acylhydrolase activity
molecular_function GO:0008970 phospholipase A1 activity