CmaCh17G002020 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G002020
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSulfate adenylyltransferase
LocationCma_Chr17: 1055807 .. 1059670 (-)
RNA-Seq ExpressionCmaCh17G002020
SyntenyCmaCh17G002020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAAAACCGTTCAACAAAGAAACAGAGAAGAACAGAACCGAAGCGGGGCCTGAGCTTGGGTCTTCACCTCCAATGGCGTCCATGGCTGCCGCCTTCATCAAACCCACAAACGCCTTCCCCCAGTCTCCAATAACCTTCGTCAAAACCATCTCACCGTCGATTCAACTCCCAATTTCTCTCAGGATTAGAAACAACGCTCGCCGTCGGATCCGGGTCTCGTGCGGGTTGATCGACCCGGATGGAGGCAAGCTTATGGACCTTATTGTGGACGAATCCATGAGGGCTTTCAAGAATCGGGAGGCTCTGAGTCTGCCCCGAGTCAAGCTGTCGCGAATCGATCTGCAATGGGTGCACATTCTGAGCGAGGGCTGGGCTAGCCCTCTTTCCGGGTTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCATGATGGATCGGTTGTTAACATGTCGGTGCCCATCGTTTTGGCCATCGACGATGCCGTCAAGACCCGAATCGGCGCCTCCTCTAAGGTTGCGCTCTTCGGCTCCGATGATCGCCCGGTTGCAATTCTCAATGAGTAAGATCACGCCTTTTGTTTTAACCGTCTTTTTTTTTTTTTCTCATTTTATATTTTATTTTTTTAAACAAAGAGTAAATTTTTTGGGCTTTTATAAATATGCCTTGTATTTAATTTTTCATATAAAAACATTATATTAAAATTTTATAATTTTCCAATTCGCATGCTACTCATATATTTTTTTTAAACCTATCGTAAGCTCGTAAACATTTGTTTTAATTAATTGATGTACGTATCGATAAATTTTATATTTAATCTACACGTTAGTTTATTGTCGAATTTTTTTAAACAAAGCCGGGACAATATTCTCTTTATCAGTATAGGATTAGAACGGCAGCTAACACGAACCAAGTTCATATATATATATTTTTTTTACTTGATTAGATAAAAATAGAATTTCTCGATCAAGAGTGATTAAACTAAAAAATTGTAATTTTTATGAAAGAGTTCGATTTTTTTAATTTTTTTTATAATAATAATTGATACGTTATTTACTATTATAGATATTGACATTTTAAGACTTAGTTTGGAATTATTGTAGCTAGGAGTGTTCGAAGGTTTTTCTTAGTACAAGTGGGTAAGAGAATTTGAACTTCCATTTTCGAGAAGATTGAACATTTTTAAACTCAATTTAATTGTTCGAGTTATAATTTTTTTTTCGAGCAGTATTGAGATATACAAGCAACCGAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTCCAGGTCTTCCTTATGTTGATGAGGCTATTAAGAATGCTGGAAATTGGTTGATAGGAGGTGATCTAGAGGTCATTGAACCAATCAAGTACCACGATGGCCTCGACTCGTTTCGATTATCGCCTGCACAACTTCGCAACGAGTTCATCAGCCGCAATGCCGATGCAGTGTTCGCTTTCCAGCTTCGCAATCCGGTGCATAACGGTCATGCTCTGCTCATGACCGATACTCGTCGACGCCTTCTTGATATGGGCTACACCAACCCTATCCTTTTGCTTAATCCATTGGGTGGCTACACCAAGGCTGATGATGTTCCTCTTCATTGGAGGATCAAACAACATCAAAAGGTACCACACCCCTCCTTTCATATCATATTTCCACAATGCTACCAACTCACTCTTGAAACTCACTTCATTAGGTTCTTGAAGATGGTGTTCTTGATCCCGAGACGACCGTTGTTTCCATTTTCCCATCGCCGATGCACTACGCCGGTCCAACCGAGGTGCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATTGTTGGTCGAGATCCTGCTGGTATGGGCCATCCTACTGAGAAGAGGGACCTTTATGATCCTGACCATGGGAAGAAAGTATTGAGCATGGCTCCTGGTTTAGAGCGCCTAAATATCCTTCCTTTCAAGGTATCATATGAACTTGCAACTTTTCTGCCACAATCACTTCATATTTCTACATATCCTTTAGTTCCAACTTCCAATTATAATGCAGGTTGCTGCATATGACAAAACTCGAGGCAAAATGGCCTTTTTTGATCCTTCAAGACCTCAGGATTTCCTCTTCATATCAGGCACCAAGGTACTTCTTCATACCAATCTCTGCAAGTCGAGAGAATCGATGTCGTTTCATAGCTTTTCCAACAGGAAATTACTGTCTTTTGTCTCTAAAACTAAAAAACAAAAGAGGGGTGTGAGATCCTACATTGGTTGGAGAGAAGAACGAAACATTTCTTATAAGGGTGTAGAAACCACTCCCTAGCAGCCGCGTTTTAAAACCTCGAGGGGAAGCCCAAAAATGACAATATTTGCTAGCGGTAGGCTTGGGCTGTTACAAATGACATCAGAGTTAGGCACCAAACGGTGTGCCAGCGAGGATGCTAGCTCCCAAGGGGGGTGGATTTTGAGATCCCACATCGGTTGGAGAAGAGAACGAAACATTCCTTAAAAGGGTGTAGAAATCTCTTCCTAGCCGACGCGTTTTAAAATTTTGAGGGGAAACCAGAAGGGAAAGGAAAGCCCAAAGAGGATAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATTAGAGCTAGGCACGAGTGTTGTGCCAGTGAGAACGCTGGGCTCCCAAGAGGGTGGATTGTGATGTCCCACATCGGTTGGAGAGGAGAATGAAGCATTCCTTATAAGGGTGTGGAAATCTCTCCCTAGCAAATATGTTTTAAAACCTTGAGGGAAAACCTAGAAGAGAAAATTCAAAGATGACAGTATCTAATAGAGGTGAGCTTGGAATATTACAGAAAATCTTTTTCATTCCTTACAAGAATCTAGGCTAGGCCTAGAAGGAACAAACTGAAACATTCTGAGTCGGGGTCTCTTCTTGGTATTTGTTAGATGCGAACTCTTGCAAGGAATAAGGAGAACCCTCCAGATGGGTTTATGTGTCCCGGTGGCTGGAGCGTATTGGTCGAATACTACGAAAGCTTAGTTCCAGCAAACATTGGGAGTATCCCCAAACCTGAAGCTGTAGCACAGCTTAAATCCTGTAGATCATAGTACATCACAGGATTTCAGCACAACACTTTATGCTCCTGTTTGTGATATAAACTTGACATTCTGTTAGGTGTCTTGAGCTGAATAAAGTCATTTGGGCATTGGCCTAGCCCTGTAACATATATGCTCACGGGCATGTAAAATTTAAGTGTGATGCATTTCGATTTTCATTTTTATGTAATATAAGTGTGATTCTAGCATAGCATGGTAAGTTTGAATTTCTCTTTCTGGACTGGTTGTCTCTTTCCGAACTGGAATACTGTGAGATCCTACGGTTGGAGTCTTATAAAAGTGTGGAATTTTTTTCCTAAAACACACATTTCAAAATCGTGAGGTTGGGTCAAAACGAACAATATCTGCTAGCAGTGGGCTTGGTTGTTTACAAACGATATCAGAGTCAGTCATCGAGGTGGTGTGCCAGTGAGGACGTTGGGCTCCAAGAGGGGTGGATTATGAGATCGCACATTGGTTGGAGAGGGGAACTAAACATTCCTTACAAAAATGTGGAAACCTCTCCCTGGAAGACGTGTTTTACGAGGATTAAAGCGGATAATATCTACTAGCGGTAAGCAGATGAATTGGTTAGAAGGCTTTACTCTAGAGGATGGGACCAAAAGTTCTCTTATAGGCTAACTTTTTTATGGAAGTATTTGTCTTTCGCTGATCTTATTGTGTAAAAAGATAGGAGTTTCATTTGTGGCTGTGTATTAACCTTCAGGCATTGACTTCCGTGTTCATATTGAAGCAGGA

mRNA sequence

ATTAAAACCGTTCAACAAAGAAACAGAGAAGAACAGAACCGAAGCGGGGCCTGAGCTTGGGTCTTCACCTCCAATGGCGTCCATGGCTGCCGCCTTCATCAAACCCACAAACGCCTTCCCCCAGTCTCCAATAACCTTCGTCAAAACCATCTCACCGTCGATTCAACTCCCAATTTCTCTCAGGATTAGAAACAACGCTCGCCGTCGGATCCGGGTCTCGTGCGGGTTGATCGACCCGGATGGAGGCAAGCTTATGGACCTTATTGTGGACGAATCCATGAGGGCTTTCAAGAATCGGGAGGCTCTGAGTCTGCCCCGAGTCAAGCTGTCGCGAATCGATCTGCAATGGGTGCACATTCTGAGCGAGGGCTGGGCTAGCCCTCTTTCCGGGTTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCATGATGGATCGGTTGTTAACATGTCGGTGCCCATCGTTTTGGCCATCGACGATGCCGTCAAGACCCGAATCGGCGCCTCCTCTAAGGTTGCGCTCTTCGGCTCCGATGATCGCCCGGTTGCAATTCTCAATGATATTGAGATATACAAGCAACCGAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTCCAGGTCTTCCTTATGTTGATGAGGCTATTAAGAATGCTGGAAATTGGTTGATAGGAGGTGATCTAGAGGTCATTGAACCAATCAAGTACCACGATGGCCTCGACTCGTTTCGATTATCGCCTGCACAACTTCGCAACGAGTTCATCAGCCGCAATGCCGATGCAGTGTTCGCTTTCCAGCTTCGCAATCCGGTGCATAACGGTCATGCTCTGCTCATGACCGATACTCGTCGACGCCTTCTTGATATGGGCTACACCAACCCTATCCTTTTGCTTAATCCATTGGGTGGCTACACCAAGGCTGATGATGTTCCTCTTCATTGGAGGATCAAACAACATCAAAAGGTTCTTGAAGATGGTGTTCTTGATCCCGAGACGACCGTTGTTTCCATTTTCCCATCGCCGATGCACTACGCCGGTCCAACCGAGGTGCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATTGTTGGTCGAGATCCTGCTGGTATGGGCCATCCTACTGAGAAGAGGGACCTTTATGATCCTGACCATGGGAAGAAAGTATTGAGCATGGCTCCTGGTTTAGAGCGCCTAAATATCCTTCCTTTCAAGGTTGCTGCATATGACAAAACTCGAGGCAAAATGGCCTTTTTTGATCCTTCAAGACCTCAGGATTTCCTCTTCATATCAGGCACCAAGATGCGAACTCTTGCAAGGAATAAGGAGAACCCTCCAGATGGGTTTATGTGTCCCGGTGGCTGGAGCGTATTGGTCGAATACTACGAAAGCTTAGTTCCAGCAAACATTGGGAGTATCCCCAAACCTGAAGCTGTAGCACAGCTTAAATCCTGTAGATCATAGTACATCACAGGATTTCAGCACAACACTTTATGCTCCTGTTTGTGATATAAACTTGACATTCTGTTAGGTGTCTTGAGCTGAATAAAGTCATTTGGGCATTGGCCTAGCCCTGTAACATATATGCTCACGGGCATGTAAAATTTAAGTGTGATGCATTTCGATTTTCATTTTTATGTAATATAAGTGTGATTCTAGCATAGCATGGTAAGTTTGAATTTCTCTTTCTGGACTGGTTGTCTCTTTCCGAACTGGAATACTGTGAGATCCTACGGTTGGAGTCTTATAAAAGTGTGGAATTTTTTTCCTAAAACACACATTTCAAAATCGTGAGGTTGGGTCAAAACGAACAATATCTGCTAGCAGTGGGCTTGGTTGTTTACAAACGATATCAGAGTCAGTCATCGAGGTGGTGTGCCAGTGAGGACGTTGGGCTCCAAGAGGGGTGGATTATGAGATCGCACATTGGTTGGAGAGGGGAACTAAACATTCCTTACAAAAATGTGGAAACCTCTCCCTGGAAGACGTGTTTTACGAGGATTAAAGCGGATAATATCTACTAGCGGTAAGCAGATGAATTGGTTAGAAGGCTTTACTCTAGAGGATGGGACCAAAAGTTCTCTTATAGGCTAACTTTTTTATGGAAGTATTTGTCTTTCGCTGATCTTATTGTGTAAAAAGATAGGAGTTTCATTTGTGGCTGTGTATTAACCTTCAGGCATTGACTTCCGTGTTCATATTGAAGCAGGA

Coding sequence (CDS)

ATGGCGTCCATGGCTGCCGCCTTCATCAAACCCACAAACGCCTTCCCCCAGTCTCCAATAACCTTCGTCAAAACCATCTCACCGTCGATTCAACTCCCAATTTCTCTCAGGATTAGAAACAACGCTCGCCGTCGGATCCGGGTCTCGTGCGGGTTGATCGACCCGGATGGAGGCAAGCTTATGGACCTTATTGTGGACGAATCCATGAGGGCTTTCAAGAATCGGGAGGCTCTGAGTCTGCCCCGAGTCAAGCTGTCGCGAATCGATCTGCAATGGGTGCACATTCTGAGCGAGGGCTGGGCTAGCCCTCTTTCCGGGTTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCATGATGGATCGGTTGTTAACATGTCGGTGCCCATCGTTTTGGCCATCGACGATGCCGTCAAGACCCGAATCGGCGCCTCCTCTAAGGTTGCGCTCTTCGGCTCCGATGATCGCCCGGTTGCAATTCTCAATGATATTGAGATATACAAGCAACCGAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTCCAGGTCTTCCTTATGTTGATGAGGCTATTAAGAATGCTGGAAATTGGTTGATAGGAGGTGATCTAGAGGTCATTGAACCAATCAAGTACCACGATGGCCTCGACTCGTTTCGATTATCGCCTGCACAACTTCGCAACGAGTTCATCAGCCGCAATGCCGATGCAGTGTTCGCTTTCCAGCTTCGCAATCCGGTGCATAACGGTCATGCTCTGCTCATGACCGATACTCGTCGACGCCTTCTTGATATGGGCTACACCAACCCTATCCTTTTGCTTAATCCATTGGGTGGCTACACCAAGGCTGATGATGTTCCTCTTCATTGGAGGATCAAACAACATCAAAAGGTTCTTGAAGATGGTGTTCTTGATCCCGAGACGACCGTTGTTTCCATTTTCCCATCGCCGATGCACTACGCCGGTCCAACCGAGGTGCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATTGTTGGTCGAGATCCTGCTGGTATGGGCCATCCTACTGAGAAGAGGGACCTTTATGATCCTGACCATGGGAAGAAAGTATTGAGCATGGCTCCTGGTTTAGAGCGCCTAAATATCCTTCCTTTCAAGGTTGCTGCATATGACAAAACTCGAGGCAAAATGGCCTTTTTTGATCCTTCAAGACCTCAGGATTTCCTCTTCATATCAGGCACCAAGATGCGAACTCTTGCAAGGAATAAGGAGAACCCTCCAGATGGGTTTATGTGTCCCGGTGGCTGGAGCGTATTGGTCGAATACTACGAAAGCTTAGTTCCAGCAAACATTGGGAGTATCCCCAAACCTGAAGCTGTAGCACAGCTTAAATCCTGTAGATCATAG

Protein sequence

MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKLMDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVAQLKSCRS
Homology
BLAST of CmaCh17G002020 vs. ExPASy Swiss-Prot
Match: Q9LIK9 (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 4.4e-216
Identity = 367/464 (79.09%), Postives = 398/464 (85.78%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLP---ISLRIRNNARRRIRVSCGLIDPDG 60
           MASMAA   K    F   P+T     SP+  LP   +S   ++  RR   +  GLI PDG
Sbjct: 1   MASMAAVLSK--TPFLSQPLT---KSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDG 60

Query: 61  GKLMDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTL 120
           GKL++LIV+E  R  K  EA  LPRV+L+ IDLQW+H+LSEGWASPL GFMRESEFLQTL
Sbjct: 61  GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTL 120

Query: 121 HFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPK 180
           HFNSLRL DGSVVNMSVPIVLAIDD  K RIG S++VALF SD  PVAIL+DIEIYK PK
Sbjct: 121 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPK 180

Query: 181 EERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEF 240
           EERIARTWGTTAPGLPYVDEAI NAGNWLIGGDLEV+EP+KY+DGLD FRLSPA+LR E 
Sbjct: 181 EERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKEL 240

Query: 241 ISRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHW 300
             RNADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLL+PLGG+TKADDVPL W
Sbjct: 241 EKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDW 300

Query: 301 RIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           R+KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFIS 420
           GHP EKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSRPQDFLFIS
Sbjct: 361 GHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS 420

Query: 421 GTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPK 462
           GTKMRTLA+N ENPPDGFMCPGGW VLV+YYESL PA  G +P+
Sbjct: 421 GTKMRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of CmaCh17G002020 vs. ExPASy Swiss-Prot
Match: O23324 (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 2.6e-208
Identity = 349/461 (75.70%), Postives = 385/461 (83.51%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASM+  F KPT+   Q      K+ S +  +      +  + R I V  GLI+PDGGKL
Sbjct: 1   MASMSTVFPKPTSFISQPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           +DL+V E  R  K  EA  LPRV+L+ IDLQW+H+LSEGWASPL GFMRESEFLQTLHFN
Sbjct: 61  VDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
            L L DGSVVNMSVPIVLAIDD  K  IG S +V+L  SDD P+AILNDIEIYK PKEER
Sbjct: 121 LLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTAPGLPYV+EAI NAG+WLIGGDLEV+EP+KY+DGLD FRLSP +LR E   R
Sbjct: 181 IARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKELEKR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
            ADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLL+PLGG+TKADDVPL WR+K
Sbjct: 241 GADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSWRMK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSR QDFLFISGTK
Sbjct: 361 VEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPK 462
           MR LA+N+ENPPDGFMCPGGW VLV+YY+SL       +P+
Sbjct: 421 MRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of CmaCh17G002020 vs. ExPASy Swiss-Prot
Match: Q9S7D8 (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 7.7e-205
Identity = 355/471 (75.37%), Postives = 394/471 (83.65%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRI----RNNARRRIRVSCGLIDPD 60
           MAS AAA +   + F  SP+  +     S   P S+ +    R  +RR + V  GLI+PD
Sbjct: 1   MASSAAAIVS-GSPFRSSPL--IHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPD 60

Query: 61  GGKLMDLIVDESMRAFKNREALSLP-RVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQ 120
           GGKLM+L+V+ES R     EA ++P R+KL+R+DL+WVH+LSEGWASPL GFMR+SEFLQ
Sbjct: 61  GGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQ 120

Query: 121 TLHFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQ 180
           TLHFNS RL DGSVVNMSVPIVLAIDD  K RIG S++V L  S   P+AILNDIEIYK 
Sbjct: 121 TLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKH 180

Query: 181 PKEERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRN 240
           PKEERIARTWGTTA GLPY +EAI  AGNWLIGGDL+V+EPIKY+DGLD FRLSP+QLR 
Sbjct: 181 PKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLRE 240

Query: 241 EFISRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPL 300
           EFI R ADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NP+LLLNPLGG+TKADDVPL
Sbjct: 241 EFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVPL 300

Query: 301 HWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPA 360
            WR++QH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPA
Sbjct: 301 SWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPA 360

Query: 361 GMGHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLF 420
           GMGHPTEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSR QDFLF
Sbjct: 361 GMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFLF 420

Query: 421 ISGTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           ISGTKMR LA+ KENPPDGFMCP GW VLV+YY+SL  A  G+    EAVA
Sbjct: 421 ISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSL-SAETGNGRVSEAVA 467

BLAST of CmaCh17G002020 vs. ExPASy Swiss-Prot
Match: Q43870 (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.2e-186
Identity = 312/447 (69.80%), Postives = 367/447 (82.10%), Query Frame = 0

Query: 10  KPTNAFPQS---PITFVKTI--SPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKLMDLI 69
           +P N+ P S    I+F+ +   +P + L     +   +  ++ V   LIDPDGG+L++LI
Sbjct: 17  QPRNSKPSSFTNQISFLSSSNNNPFLNLVYKRNLTMQSVSKMTVKSSLIDPDGGELVELI 76

Query: 70  VDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFNSLRL 129
           V E+    K  E+ ++P+VKL++IDL+WVH++SEGWASPL GFMRE E+LQ+LHFNSLRL
Sbjct: 77  VPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPLKGFMREDEYLQSLHFNSLRL 136

Query: 130 HDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEERIART 189
            +G+ VNMS+PIVLAIDD  K +IG+S  VAL       +  L  +EIYK  KEERIART
Sbjct: 137 KNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLRSVEIYKHNKEERIART 196

Query: 190 WGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISRNADA 249
           WGTT+PGLPYV+E I  +GNWLIGGDLEV EPIKY+DGLD +RLSP QLR EF +R ADA
Sbjct: 197 WGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLDHYRLSPKQLREEFDNRQADA 256

Query: 250 VFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIKQHQK 309
           VFAFQLRNPVHNGHALLM DTR+RLL+MGY NP+LLL+PLGG+TKADDVPL  R++QH K
Sbjct: 257 VFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKADDVPLDVRMEQHSK 316

Query: 310 VLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKR 369
           VLEDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKR
Sbjct: 317 VLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKR 376

Query: 370 DLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTKMRTL 429
           DLYDPDHGK+VLSMAPGLE+LNILPF+VAAYD    KMAFFDPSR ++FLFISGTKMRT 
Sbjct: 377 DLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAFFDPSRAKEFLFISGTKMRTY 436

Query: 430 ARNKENPPDGFMCPGGWSVLVEYYESL 452
           AR  ENPPDGFMCP GW+VLV+YYESL
Sbjct: 437 ARTGENPPDGFMCPSGWNVLVKYYESL 463

BLAST of CmaCh17G002020 vs. ExPASy Swiss-Prot
Match: O95340 (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX=9606 GN=PAPSS2 PE=1 SV=2)

HSP 1 Score: 499.6 bits (1285), Expect = 3.9e-140
Identity = 235/390 (60.26%), Postives = 298/390 (76.41%), Query Frame = 0

Query: 62  DLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFNS 121
           +L V E+       EA +LP + ++++DLQWV +LSEGWA+PL GFMRE E+LQ +HF++
Sbjct: 226 ELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDT 285

Query: 122 LRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEERI 181
           L L DG V+NMS+PIVL +    KTR+   SK  L     R VAIL D E Y+  KEER 
Sbjct: 286 L-LDDG-VINMSIPIVLPVSAEDKTRLEGCSKFVL-AHGGRRVAILRDAEFYEHRKEERC 345

Query: 182 ARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISRN 241
           +R WGTT    P++ + +  +G+WL+GGDL+V+E I+++DGLD +RL+P +L+ +    N
Sbjct: 346 SRVWGTTCTKHPHI-KMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKEMN 405

Query: 242 ADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIKQ 301
           ADAVFAFQLRNPVHNGHALLM DTRRRLL+ GY +P+LLL+PLGG+TK DDVPL WR+KQ
Sbjct: 406 ADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRMKQ 465

Query: 302 HQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPT 361
           H  VLE+GVLDP++T+V+IFPSPM YAGPTEVQWH ++R+ AGANFYIVGRDPAGM HP 
Sbjct: 466 HAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPHPE 525

Query: 362 EKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTKM 421
            K+DLY+P HG KVLSMAPGL  + I+PF+VAAY+K +  M F+DP+R  +F FISGT+M
Sbjct: 526 TKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNEFDFISGTRM 585

Query: 422 RTLARNKENPPDGFMCPGGWSVLVEYYESL 452
           R LAR  ENPPDGFM P  W VL +YY SL
Sbjct: 586 RKLAREGENPPDGFMAPKAWKVLTDYYRSL 611

BLAST of CmaCh17G002020 vs. ExPASy TrEMBL
Match: A0A6J1L5Q7 (Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499372 PE=4 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 7.6e-280
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVAQLKSCRS 474
           MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVAQLKSCRS
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVAQLKSCRS 473

BLAST of CmaCh17G002020 vs. ExPASy TrEMBL
Match: A0A6J1H3X2 (Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111459921 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.1e-268
Identity = 451/466 (96.78%), Postives = 459/466 (98.50%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKP+NAFP SPITF+KT SPSI+LPISLRI+NNA RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           +DLIVDESMRAFKNREALSLPR+KLSRIDLQWVH+LSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRIKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDR VAILNDIEIYKQPKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
           NA AVFAFQLRNPVHNGHALLM DTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NAGAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPAN GSIPKPEAVA
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVA 466

BLAST of CmaCh17G002020 vs. ExPASy TrEMBL
Match: A0A0A0LIM7 (Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G215520 PE=4 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 1.8e-252
Identity = 423/466 (90.77%), Postives = 439/466 (94.21%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKP+N FPQSPITF K ++P I LP+SLR++    RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           ++LIV+ESMR  KNREALSLPR+KLSRIDLQWVH+LSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
           SLRL DGSVVNMSVPIVLAIDD  K RIG SSKVALFGSDDRPVAILNDIEIYK PKEER
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDFKNRIGDSSKVALFGSDDRPVAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTA GLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF SR
Sbjct: 181 IARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           MR LARNKENPPDGFMCP GW+VLVEYY+SLVPA  G IPKPEAVA
Sbjct: 421 MRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPAENGRIPKPEAVA 466

BLAST of CmaCh17G002020 vs. ExPASy TrEMBL
Match: A0A1S3BMN3 (Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103491225 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.7e-251
Identity = 421/466 (90.34%), Postives = 443/466 (95.06%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKP+N F +SPITF K ++P I+LP+SLR+++N RRRIRVS GLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNPFSKSPITFSKAVTPPIKLPMSLRVKSNTRRRIRVSGGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           ++LIV+ESMR  KNREALSLPR+KLSRID+QWVH+LSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDVQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
           SLRL DGSVVNMSVPIVLAIDD +K RIG SSKVALFGSDDRPVAILNDIEIYK PKEER
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSKVALFGSDDRPVAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTA GLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF SR
Sbjct: 181 IARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTSR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGTK
Sbjct: 361 IEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           MR LARNKENPPDGFMCPGGW+VLVEYY+SLVPA+ G IPKPEAVA
Sbjct: 421 MRNLARNKENPPDGFMCPGGWNVLVEYYKSLVPADNGRIPKPEAVA 466

BLAST of CmaCh17G002020 vs. ExPASy TrEMBL
Match: A0A6J1CE70 (Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111009889 PE=4 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 2.8e-234
Identity = 400/467 (85.65%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNA--RRRIRVSCGLIDPDGG 60
           MASMAA F KP++   Q+P+ F K   P    P +LR+R N    R++RVS GLI+PDGG
Sbjct: 1   MASMAAVFCKPSH---QAPLRFTKI--PPSPPPPALRVRTNTTHHRQLRVSAGLIEPDGG 60

Query: 61  KLMDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLH 120
           KL++L+VDESMR  K REALS PR+KLSRIDLQWVH+LSEGWA+PLSGFMRE EFLQTLH
Sbjct: 61  KLVELLVDESMRGLKKREALSQPRIKLSRIDLQWVHVLSEGWATPLSGFMREPEFLQTLH 120

Query: 121 FNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKE 180
           FNSLRL    VVNMSVPIVLAIDDAVK RIGASS VALF SDDRPVAIL DIEIYK PKE
Sbjct: 121 FNSLRLAGAGVVNMSVPIVLAIDDAVKHRIGASSDVALFASDDRPVAILKDIEIYKHPKE 180

Query: 181 ERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFI 240
           ERIARTWGTTA GLPY DEAIKNAGNWLIGGDLEVIEPIKYHDGLD FRLSPA+LR EF 
Sbjct: 181 ERIARTWGTTASGLPYADEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAELRKEFT 240

Query: 241 SRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWR 300
            RNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGY NPILLLNPLGGYTKADDVPL+WR
Sbjct: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYENPILLLNPLGGYTKADDVPLNWR 300

Query: 301 IKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           +KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISG 420
           HPTEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISG
Sbjct: 361 HPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISG 420

Query: 421 TKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAV 466
           TKMRTLARNKENPPDGFMCPGGWSVLVEYY+SL+PAN G IPKPEAV
Sbjct: 421 TKMRTLARNKENPPDGFMCPGGWSVLVEYYDSLIPANNGRIPKPEAV 462

BLAST of CmaCh17G002020 vs. NCBI nr
Match: XP_023006723.1 (ATP sulfurylase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 972.2 bits (2512), Expect = 1.6e-279
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVAQLKSCRS 474
           MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVAQLKSCRS
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVAQLKSCRS 473

BLAST of CmaCh17G002020 vs. NCBI nr
Match: XP_023548667.1 (ATP sulfurylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941.0 bits (2431), Expect = 3.9e-270
Identity = 456/466 (97.85%), Postives = 461/466 (98.93%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKP+NAFP SPITFVKT SP IQLPISLRI+NNARRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFVKTTSPWIQLPISLRIKNNARRRIRVSCGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           +DLIVDESMRAFKNREALSLPRVKLSRIDLQWVH+LSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPAN G IPKPEAVA
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGRIPKPEAVA 466

BLAST of CmaCh17G002020 vs. NCBI nr
Match: KAG6574955.1 (ATP sulfurylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 936.0 bits (2418), Expect = 1.2e-268
Identity = 453/466 (97.21%), Postives = 460/466 (98.71%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKP+NAFP SPITF+KT SPSI+LPISLRI+NNA RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           +DLIVDESMRAFKNREALSLPRVKLSRIDLQWVH+LSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDR VAILNDIEIYKQPKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
           NADAVFAFQLRNPVHNGHALLM DTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPAN GSIPKPEAVA
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVA 466

BLAST of CmaCh17G002020 vs. NCBI nr
Match: XP_022958733.1 (ATP sulfurylase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 932.9 bits (2410), Expect = 1.1e-267
Identity = 451/466 (96.78%), Postives = 459/466 (98.50%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKP+NAFP SPITF+KT SPSI+LPISLRI+NNA RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           +DLIVDESMRAFKNREALSLPR+KLSRIDLQWVH+LSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRIKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDR VAILNDIEIYKQPKEER
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDIEIYKQPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR
Sbjct: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
           NA AVFAFQLRNPVHNGHALLM DTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK
Sbjct: 241 NAGAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
           TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGTK
Sbjct: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPAN GSIPKPEAVA
Sbjct: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVA 466

BLAST of CmaCh17G002020 vs. NCBI nr
Match: KAG7013521.1 (ATP sulfurylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 930.6 bits (2404), Expect = 5.2e-267
Identity = 453/467 (97.00%), Postives = 459/467 (98.29%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKP+N FP SPITF+KT SPSI+LPISLRI+NNA RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           +DLIVDESMRAFKNREALSLPRVKLSRIDLQWVH+LSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILND-IEIYKQPKEE 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDR VAILND IEIYKQPKEE
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS
Sbjct: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNADAVFAFQLRNPVHNGHALLM DTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI
Sbjct: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT
Sbjct: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPAN GSIPKPEAVA
Sbjct: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVA 467

BLAST of CmaCh17G002020 vs. TAIR 10
Match: AT3G22890.1 (ATP sulfurylase 1 )

HSP 1 Score: 751.9 bits (1940), Expect = 3.1e-217
Identity = 367/464 (79.09%), Postives = 398/464 (85.78%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLP---ISLRIRNNARRRIRVSCGLIDPDG 60
           MASMAA   K    F   P+T     SP+  LP   +S   ++  RR   +  GLI PDG
Sbjct: 1   MASMAAVLSK--TPFLSQPLT---KSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDG 60

Query: 61  GKLMDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTL 120
           GKL++LIV+E  R  K  EA  LPRV+L+ IDLQW+H+LSEGWASPL GFMRESEFLQTL
Sbjct: 61  GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTL 120

Query: 121 HFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPK 180
           HFNSLRL DGSVVNMSVPIVLAIDD  K RIG S++VALF SD  PVAIL+DIEIYK PK
Sbjct: 121 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPK 180

Query: 181 EERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEF 240
           EERIARTWGTTAPGLPYVDEAI NAGNWLIGGDLEV+EP+KY+DGLD FRLSPA+LR E 
Sbjct: 181 EERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKEL 240

Query: 241 ISRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHW 300
             RNADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLL+PLGG+TKADDVPL W
Sbjct: 241 EKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDW 300

Query: 301 RIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           R+KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFIS 420
           GHP EKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSRPQDFLFIS
Sbjct: 361 GHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFIS 420

Query: 421 GTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPK 462
           GTKMRTLA+N ENPPDGFMCPGGW VLV+YYESL PA  G +P+
Sbjct: 421 GTKMRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of CmaCh17G002020 vs. TAIR 10
Match: AT4G14680.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 726.1 bits (1873), Expect = 1.8e-209
Identity = 349/461 (75.70%), Postives = 385/461 (83.51%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60
           MASM+  F KPT+   Q      K+ S +  +      +  + R I V  GLI+PDGGKL
Sbjct: 1   MASMSTVFPKPTSFISQPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGGKL 60

Query: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120
           +DL+V E  R  K  EA  LPRV+L+ IDLQW+H+LSEGWASPL GFMRESEFLQTLHFN
Sbjct: 61  VDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEER 180
            L L DGSVVNMSVPIVLAIDD  K  IG S +V+L  SDD P+AILNDIEIYK PKEER
Sbjct: 121 LLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 240
           IARTWGTTAPGLPYV+EAI NAG+WLIGGDLEV+EP+KY+DGLD FRLSP +LR E   R
Sbjct: 181 IARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKELEKR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 300
            ADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NPILLL+PLGG+TKADDVPL WR+K
Sbjct: 241 GADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSWRMK 300

Query: 301 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 420
            EKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSR QDFLFISGTK
Sbjct: 361 VEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPK 462
           MR LA+N+ENPPDGFMCPGGW VLV+YY+SL       +P+
Sbjct: 421 MRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of CmaCh17G002020 vs. TAIR 10
Match: AT5G43780.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 714.5 bits (1843), Expect = 5.5e-206
Identity = 355/471 (75.37%), Postives = 394/471 (83.65%), Query Frame = 0

Query: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRI----RNNARRRIRVSCGLIDPD 60
           MAS AAA +   + F  SP+  +     S   P S+ +    R  +RR + V  GLI+PD
Sbjct: 1   MASSAAAIVS-GSPFRSSPL--IHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPD 60

Query: 61  GGKLMDLIVDESMRAFKNREALSLP-RVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQ 120
           GGKLM+L+V+ES R     EA ++P R+KL+R+DL+WVH+LSEGWASPL GFMR+SEFLQ
Sbjct: 61  GGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQ 120

Query: 121 TLHFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQ 180
           TLHFNS RL DGSVVNMSVPIVLAIDD  K RIG S++V L  S   P+AILNDIEIYK 
Sbjct: 121 TLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKH 180

Query: 181 PKEERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRN 240
           PKEERIARTWGTTA GLPY +EAI  AGNWLIGGDL+V+EPIKY+DGLD FRLSP+QLR 
Sbjct: 181 PKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLRE 240

Query: 241 EFISRNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPL 300
           EFI R ADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGY NP+LLLNPLGG+TKADDVPL
Sbjct: 241 EFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVPL 300

Query: 301 HWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPA 360
            WR++QH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPA
Sbjct: 301 SWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPA 360

Query: 361 GMGHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLF 420
           GMGHPTEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSR QDFLF
Sbjct: 361 GMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFLF 420

Query: 421 ISGTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 467
           ISGTKMR LA+ KENPPDGFMCP GW VLV+YY+SL  A  G+    EAVA
Sbjct: 421 ISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSL-SAETGNGRVSEAVA 467

BLAST of CmaCh17G002020 vs. TAIR 10
Match: AT1G19920.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 654.1 bits (1686), Expect = 8.8e-188
Identity = 312/447 (69.80%), Postives = 367/447 (82.10%), Query Frame = 0

Query: 10  KPTNAFPQS---PITFVKTI--SPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKLMDLI 69
           +P N+ P S    I+F+ +   +P + L     +   +  ++ V   LIDPDGG+L++LI
Sbjct: 17  QPRNSKPSSFTNQISFLSSSNNNPFLNLVYKRNLTMQSVSKMTVKSSLIDPDGGELVELI 76

Query: 70  VDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFNSLRL 129
           V E+    K  E+ ++P+VKL++IDL+WVH++SEGWASPL GFMRE E+LQ+LHFNSLRL
Sbjct: 77  VPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPLKGFMREDEYLQSLHFNSLRL 136

Query: 130 HDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILNDIEIYKQPKEERIART 189
            +G+ VNMS+PIVLAIDD  K +IG+S  VAL       +  L  +EIYK  KEERIART
Sbjct: 137 KNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLRSVEIYKHNKEERIART 196

Query: 190 WGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISRNADA 249
           WGTT+PGLPYV+E I  +GNWLIGGDLEV EPIKY+DGLD +RLSP QLR EF +R ADA
Sbjct: 197 WGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLDHYRLSPKQLREEFDNRQADA 256

Query: 250 VFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIKQHQK 309
           VFAFQLRNPVHNGHALLM DTR+RLL+MGY NP+LLL+PLGG+TKADDVPL  R++QH K
Sbjct: 257 VFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKADDVPLDVRMEQHSK 316

Query: 310 VLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKR 369
           VLEDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKR
Sbjct: 317 VLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKR 376

Query: 370 DLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTKMRTL 429
           DLYDPDHGK+VLSMAPGLE+LNILPF+VAAYD    KMAFFDPSR ++FLFISGTKMRT 
Sbjct: 377 DLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAFFDPSRAKEFLFISGTKMRTY 436

Query: 430 ARNKENPPDGFMCPGGWSVLVEYYESL 452
           AR  ENPPDGFMCP GW+VLV+YYESL
Sbjct: 437 ARTGENPPDGFMCPSGWNVLVKYYESL 463

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIK94.4e-21679.09ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV... [more]
O233242.6e-20875.70ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV... [more]
Q9S7D87.7e-20575.37ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV... [more]
Q438701.2e-18669.80ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1[more]
O953403.9e-14060.26Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
A0A6J1L5Q77.6e-280100.00Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499372 PE=4 SV=... [more]
A0A6J1H3X25.1e-26896.78Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111459921 PE=4 S... [more]
A0A0A0LIM71.8e-25290.77Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G215520 PE=4 SV=1[more]
A0A1S3BMN35.7e-25190.34Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103491225 PE=4 SV=1[more]
A0A6J1CE702.8e-23485.65Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111009889 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_023006723.11.6e-279100.00ATP sulfurylase 1, chloroplastic-like [Cucurbita maxima][more]
XP_023548667.13.9e-27097.85ATP sulfurylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6574955.11.2e-26897.21ATP sulfurylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022958733.11.1e-26796.78ATP sulfurylase 1, chloroplastic-like [Cucurbita moschata][more]
KAG7013521.15.2e-26797.00ATP sulfurylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT3G22890.13.1e-21779.09ATP sulfurylase 1 [more]
AT4G14680.11.8e-20975.70Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT5G43780.15.5e-20675.37Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT1G19920.18.8e-18869.80Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 220..454
e-value: 1.1E-93
score: 314.8
IPR025980ATP-sulfurylase PUA-like domainPFAMPF14306PUA_2coord: 53..215
e-value: 8.6E-43
score: 145.8
IPR024951Sulphate adenylyltransferase catalytic domainPFAMPF01747ATP-sulfurylasecoord: 225..447
e-value: 2.8E-65
score: 219.8
NoneNo IPR availableGENE3D3.10.400.10Sulfate adenylyltransferasecoord: 51..219
e-value: 9.8E-56
score: 189.8
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 1..462
NoneNo IPR availablePANTHERPTHR11055:SF41ATP SULFURYLASE 1, CHLOROPLASTICcoord: 1..462
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 225..448
IPR002650Sulphate adenylyltransferaseTIGRFAMTIGR00339TIGR00339coord: 55..446
e-value: 2.4E-134
score: 445.9
IPR002650Sulphate adenylyltransferaseCDDcd00517ATPScoord: 80..447
e-value: 6.69666E-163
score: 462.487
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 55..219

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G002020.1CmaCh17G002020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004781 sulfate adenylyltransferase (ATP) activity