CmaCh16G010760.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G010760.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	beta-amyrin 11-oxidase-like
Location	Cma_Chr16: 8246109 .. 8249312 (-)
Sequence length	288
RNA-Seq Expression	CmaCh16G010760.1
Synteny	CmaCh16G010760.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCGTGTAAATTATAGCTTAGAACAATTGATTCCTTTTCTTTTCTTATATAGGCTAGAAAGAAGTTGCTCAACATACTTCAATCCATCATCAACCAAAAGAGATTGGCGAAGAACATGCAAGGAAAAAGTTGGGAAGCCAAAGACATGATGGATCTGCTTATGGACGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTATAGATTTGATATTTGGTAACTTATTTGCAGGCCATGAAACTTCATCTATTACAGCAATGTGGGCTGTTTTGTTTCTTACAAACCATCCACATGTTTTTCAAAGAGCCAAGGTTAGTAATTTTAAGGATTAAAAATTACAATAATATTGTAAACTTTTAATATAAGTTGAAAATACTTTTAGTGTTAGTATTTTTACGGTTAGTACAATGTTTTAAAAATATTCATAAACTTTCTAGAGTAACGTTAAAATGTATGTAAATAATCTATGATATAAATGTTTTACATCCACATATTGATGGTAAATTTGTTTTTGAATTTTTTACTAAAGTTAAAATGGGGTTAATGCTACTATTAAAATTTTATCGATTTTTTTTAAATATAGTATTAACGGTAAAAATATTTTTGAATAGTTTAAAAAAAGTTTAAAGGTATTAATAAAATTTTTTAAATGTTAAGAATGAGAAGTTCGGATACCATGTTAATATTAAGAATGATGCTTTAAATACCATGTTATGATGCTCTAATTGTATTAAGAATGATGCACGGTATCATAGCATCACTTTTAAGAACATTTTTTAAAAAAGAGTACTAACCGTTTTCATCTAAAATTAACCGTGTATCTAAAATTAACCGTGTAATTGTATTAAGAATGATGCACGGTATCCAGTTGATGTTGTCAAGAAATGTGGCATTCAACATCATCAAGGAGAAAACAATGCCAAATCTGTTGAAGGCGCTGTCAAATGTGTATGAAAAATCGTCGGCTATAAACATGGTGTATTTGATGCGAAGATTTTTCATTTTTCTAATGTCTGAAGGTGGATCTGTTGCAGATCATATAAATGAATTCAATATGATTATAAGTCAACTAAGTTTGGTGAGCATTAATTTTGAAGATAAAATTAAAGCATTGATTTTGATGTCATTTTTACCCGAGTCGTGTAATATTGTTATTGTTATAATTAGCAGTTTTTGAGAATTTGAAAAAAACTGAAGTTCGATGAAATTCGCAATGTAGTTCTTAGCGAAAGTATTCGGAAACGAGATATTATGGATTCATCATGCAATGCTCTCAGTGTTGATCCAATGGGAAGAAGTAAATTAGATTGCCCAAACAAAGGCGAATCAAAACCAAAAAGCTTACAAAAAAAAAAAAATATTCCAAACAAACCAAACATAACATGTTGGAATTGTGGAGAAAAAGGTCGAACAGATTGTACAAGACCAAAGAGGAAACAGAATCACAAATTAGAGGAGGATAACCATTCTATAAATGTTATAATTCTTGGATAGTTATGTTGAATCTTGGATTTTGGATTCTGGTGAATCTTTTCATTCGCCCCTAAATAAGGAGTTGTTTTTTTAATTTCAAATCCGAAAACAAAGTCTTGGAGATTAAATGAAAGGGAGATCCTTTCATAAAAACTCCTGAAGGAGTTCAGTGGACATTAAAGGATATCAAATATATTCATGGTCAAAAGAACCCCATCTCTATTGTTGAGTTGGACAGCACATGTTATGCAATAGAGTTTAAGAAGAGTTCACGGAAGATTGTGAAGGGTGTTATGGTGGTAGTACGTGGCCTAAAATCTGGAACCTTATACCCCACTTCAGGGTGTATGAACATGGTTGTTGTTGCTAAGAGTGCTTTCAATTCAAGTCTATGGCACAATTGACTTGGACATATGAACGTCAAAAGAGTAAAGATGTTGGCTGTGAACAGAGTTTTAGAAGGTTTGAAATTTGTTGACATGGGTCCTTGTGAGATTTGCGTTATGAGCAAACAAAAACGAGTTAGTTTCACAAAAGCTACTAGAGAATTGAAGAAAATGTGGTTGGGAATGGTCCATACAGACGTTTAAGGACTATCTCTAGTTTCATCACTTGGTGGATCAAAGTTCTATGTCACCTTTATCGGTGATTTCAGCATAAAAGTGTGAATTTGTTTCTTGAAACACAAGAAGTGGAAAGCTGAAGTTGAAAATCAAACCAGCTTGAAGATCAAATGTCTGAGGTCTAACAATGGAGAAGAGTATAACAAGTCAGAATTCAAAGCATTTTGTGCAACTGAGAGAATTAGACAAAGGAGAACAATTTCCAATAAAGCAAGACAAAATAGTGGTGTTGAAAGGATGAACAAAACATTGAATGAATAGGCAAGGAGTATTATGATTCATTTTGAATTGCCAAGGACATTCTAGGTCGATACTGTAAATACAACAGTCTATTTGATCAATAGAGAGTCGTCAGTACCCTTAGAGTTCAAGTTGTTAGAAGAAGTATGGGCAGAAAAAGAACTCAAATATTATCTGAATTGCATATGTTCATGTTGATCCAAAAAAAAGTGACAAGTTTGATGTTGAGGTTTTGAAGTGCCTAGGCTAAGGTTCTTACATGTTCGAGTACATGTTTTTGGATGACAAGAACAGAAAAGTCCTTAGACATTGTGACGTGACTTTTGATGAAAATGTCCGGTACAAGAACAAAAAGAAGAAGAATTTCAAGACAACGAAGCAAGTGGGAGTTGAGGTTGAGTTGTAGAAAGATTCACCTAGTAATGTTGTAGCAGTTACTAAAAAAAAAACTCCTGAGACTATTATCGAGGAACCAAAGGTGGAGCATGTGACACCTCAGCAGCTGTTAATAAGATCATCTAGAAATATTAGAGTACGATATAGTTATTCACCCTCAGCACACTATTTGTTGCTGATTGATAAAGGAAAACCAGAGTCCCTCTATGATGTCGTAATACAACCAAGTGGTAACAAGTCATGGATGCTAAGAGGAGTTGGATGTCTATGTTTTAAAATAAGATAATTGTGTTTGATGTTGCAAGAGGAGGGAATAGGATGCTTGAGAATCAGATAACTGTATTTGGTATTGCAAGATAAACGTTATCAAAATATATATGGAAATCGAGTTGCAAATCATAATTCAATCTAGCTATTTTTTATGGGACTTTTATCTTTTCTTTTAGGTTCCCCTTTTTTGA

mRNA sequence

ATGTTCGCTAGAAAGAAGTTGCTCAACATACTTCAATCCATCATCAACCAAAAGAGATTGGCGAAGAACATGCAAGGAAAAAGTTGGGAAGCCAAAGACATGATGGATCTGCTTATGGACGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTATAGATTTGATATTTGGTAACTTATTTGCAGGCCATGAAACTTCATCTATTACAGCAATGTGGGCTGTTTTGTTTCTTACAAACCATCCACATGTTTTTCAAAGAGCCAAGGTTCCCCTTTTTTGA

Coding sequence (CDS)

ATGTTCGCTAGAAAGAAGTTGCTCAACATACTTCAATCCATCATCAACCAAAAGAGATTGGCGAAGAACATGCAAGGAAAAAGTTGGGAAGCCAAAGACATGATGGATCTGCTTATGGACGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTATAGATTTGATATTTGGTAACTTATTTGCAGGCCATGAAACTTCATCTATTACAGCAATGTGGGCTGTTTTGTTTCTTACAAACCATCCACATGTTTTTCAAAGAGCCAAGGTTCCCCTTTTTTGA

Protein sequence

MFARKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNLFAGHETSSITAMWAVLFLTNHPHVFQRAKVPLF

Homology

[Close]

BLAST of CmaCh16G010760.1 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.9e-17
Identity = 41/90 (45.56%), Postives = 69/90 (76.67%), Query Frame = 0


Query: 3   ARKKLLNILQSIINQKRL-AKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGN 62
           ARKKL  I+Q +++++RL  +N   +  + KD++D+L++VKDE+G +L+DE I DL+ G 
Sbjct: 239 ARKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGL 298

Query: 63  LFAGHETSSITAMWAVLFLTNHPHVFQRAK 92
           LFAGHE+++ + MW++ +LT HPH+ ++AK
Sbjct: 299 LFAGHESTATSLMWSITYLTQHPHILKKAK 328

[Close]

BLAST of CmaCh16G010760.1 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 3.9e-15
Identity = 46/89 (51.69%), Postives = 59/89 (66.29%), Query Frame = 0


Query: 3   ARKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNL 62
           ARK L+   QSI+ ++R  +     S   KDM+D L++VKDEDG+ LDDE IID++   L
Sbjct: 240 ARKTLVAAFQSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYL 299

Query: 63  FAGHETSSITAMWAVLFLTNHPHVFQRAK 92
            AGHE+S  T MWA +FL  HP V QRAK
Sbjct: 300 NAGHESSGHTIMWATVFLQEHPEVLQRAK 327

[Close]

BLAST of CmaCh16G010760.1 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 3.3e-14
Identity = 44/89 (49.44%), Postives = 57/89 (64.04%), Query Frame = 0


Query: 3   ARKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNL 62
           ARKKL+   QSI+  +R  +  Q  S   KDM+D L+DVKDE+G  LDDE IIDL+   L
Sbjct: 239 ARKKLVAAFQSIVTNRR-NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYL 298

Query: 63  FAGHETSSITAMWAVLFLTNHPHVFQRAK 92
            AGHE+S    MWA + +  HP + Q+AK
Sbjct: 299 NAGHESSGHLTMWATILMQEHPMILQKAK 326

[Close]

BLAST of CmaCh16G010760.1 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.1e-12
Identity = 39/89 (43.82%), Postives = 60/89 (67.42%), Query Frame = 0


Query: 3   ARKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNL 62
           AR++L+ +LQ +++++R A+  +G S    DMMD L++ +DE G  LDD+ IID++   L
Sbjct: 268 ARRRLVAVLQGVLDERRAAR-AKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYL 327

Query: 63  FAGHETSSITAMWAVLFLTNHPHVFQRAK 92
            AGHE+S    MWA +FL  +P +F RAK
Sbjct: 328 NAGHESSGHITMWATVFLQENPDMFARAK 355

[Close]

BLAST of CmaCh16G010760.1 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.1e-12
Identity = 38/89 (42.70%), Postives = 57/89 (64.04%), Query Frame = 0


Query: 3   ARKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNL 62
           AR+KL+++LQ +++ +R A     K   A DMMD L++ +DE G  L D+ I+D++   L
Sbjct: 252 ARRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYL 311

Query: 63  FAGHETSSITAMWAVLFLTNHPHVFQRAK 92
            AGHE+S    MWA +FL  +P +F RAK
Sbjct: 312 NAGHESSGHITMWATVFLQENPDIFARAK 340

[Close]

BLAST of CmaCh16G010760.1 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 82.0 bits (201), Expect = 2.8e-16
Identity = 46/89 (51.69%), Postives = 59/89 (66.29%), Query Frame = 0


Query: 3   ARKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNL 62
           ARK L+   QSI+ ++R  +     S   KDM+D L++VKDEDG+ LDDE IID++   L
Sbjct: 240 ARKTLVAAFQSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYL 299

Query: 63  FAGHETSSITAMWAVLFLTNHPHVFQRAK 92
            AGHE+S  T MWA +FL  HP V QRAK
Sbjct: 300 NAGHESSGHTIMWATVFLQEHPEVLQRAK 327

[Close]

BLAST of CmaCh16G010760.1 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 79.0 bits (193), Expect = 2.4e-15
Identity = 44/89 (49.44%), Postives = 57/89 (64.04%), Query Frame = 0


Query: 3   ARKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNL 62
           ARKKL+   QSI+  +R  +  Q  S   KDM+D L+DVKDE+G  LDDE IIDL+   L
Sbjct: 239 ARKKLVAAFQSIVTNRR-NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYL 298

Query: 63  FAGHETSSITAMWAVLFLTNHPHVFQRAK 92
            AGHE+S    MWA + +  HP + Q+AK
Sbjct: 299 NAGHESSGHLTMWATILMQEHPMILQKAK 326

[Close]

BLAST of CmaCh16G010760.1 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 79.0 bits (193), Expect = 2.4e-15
Identity = 44/89 (49.44%), Postives = 57/89 (64.04%), Query Frame = 0


Query: 3   ARKKLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNL 62
           ARKKL+   QSI+  +R  +  Q  S   KDM+D L+DVKDE+G  LDDE IIDL+   L
Sbjct: 239 ARKKLVAAFQSIVTNRR-NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYL 298

Query: 63  FAGHETSSITAMWAVLFLTNHPHVFQRAK 92
            AGHE+S    MWA + +  HP + Q+AK
Sbjct: 299 NAGHESSGHLTMWATILMQEHPMILQKAK 326

[Close]

BLAST of CmaCh16G010760.1 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 45.4 bits (106), Expect = 2.9e-05
Identity = 25/89 (28.09%), Postives = 49/89 (55.06%), Query Frame = 0


Query: 6   KLLNILQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNLFAG 65
           +L  +LQ ++++KR  K       + + ++D L+  ++ + E   D  I  +I   + AG
Sbjct: 248 RLDRVLQKLVDEKRAEKE------KGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAG 307

Query: 66  HETSSITAMWAVLFLTNHPHVFQRAKVPL 95
            +TSS+T  WA+  L NHP + ++A+  +
Sbjct: 308 TDTSSVTLEWAMSNLLNHPEILEKARAEI 330

[Close]

BLAST of CmaCh16G010760.1 vs. TAIR 10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7 )

HSP 1 Score: 45.1 bits (105), Expect = 3.8e-05
Identity = 26/84 (30.95%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 11  LQSIINQKRLAKNMQGKSWEAKDMMDLLMDVKDEDGEELDDETIIDLIFGNLFAGHETSS 70
           LQS+++++R+ K       +   MMD L+ +++   +   D T+  +I   + AG ET +
Sbjct: 297 LQSLVDERRVHKQ------KGNTMMDHLLSLQETQPDYYTDVTLKGIIIVMILAGTETLA 356

Query: 71  ITAMWAVLFLTNHPHVFQRAKVPL 95
            T  WA+L L NHP V ++A+  +
Sbjct: 357 GTLEWAMLNLLNHPEVLEKARTEI 374

The following BLAST results are available for this feature:

BLAST of CmaCh16G010760.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.9e-17	45.56	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
O23051	3.9e-15	51.69	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q9C5Y2	3.3e-14	49.44	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q43246	1.1e-12	43.82	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]
Q5VRM7	3.1e-12	42.70	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]

BLAST of CmaCh16G010760.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05160.1	2.8e-16	51.69	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G32440.1	2.4e-15	49.44	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	2.4e-15	49.44	ent-kaurenoic acid hydroxylase 2	[more]
AT4G37320.1	2.9e-05	28.09	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT2G23190.1	3.8e-05	30.95	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 3..90 e-value: 9.6E-15 score: 54.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..92 e-value: 2.1E-21 score: 78.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..90
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 2..91
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 2..91

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh16G010760	CmaCh16G010760	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh16G010760.1:exon:1077	CmaCh16G010760.1:exon:1077	exon
CmaCh16G010760.1:exon:1078	CmaCh16G010760.1:exon:1078	exon
CmaCh16G010760.1:exon:1079	CmaCh16G010760.1:exon:1079	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh16G010760.1:cds	CmaCh16G010760.1:cds_3	CDS
CmaCh16G010760.1:cds	CmaCh16G010760.1:cds_2	CDS
CmaCh16G010760.1:cds	CmaCh16G010760.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh16G010760.1	CmaCh16G010760.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen