CmaCh16G010020 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G010020
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionsubtilisin-like protease SBT5.3
LocationCma_Chr16: 7712432 .. 7722578 (+)
RNA-Seq ExpressionCmaCh16G010020
SyntenyCmaCh16G010020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATAATCTAAAATCAGAGGTAGTGAAGATTAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGGCACCACCAGGAGTTTTGGTTTCGGTAGACCCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGGATTTTCAAGTTGTGTTGAGGAGGGTTCAAAGTAATCAAACTGAAGAAAAGCATGTGTTCGAAAAACTTGTGTGGCCTGATGGAAAACATCGTGTTAGTAGTCCAATTTTTGTGACATTAGAGATTTAGTAAAAGGTTGAGTTTAGCTTGTTTTAGCTTGAAAAAACAACTTGAAGGGAGAATTTTTTGGACTAAGAACCTTAACACTATGCTCTTTGATTCTCTTATGTTGTTGTAAATAGAACATACACATTAATATTTATTGATAAAACATTAACAAATTTCCTAAACTTTATCCTCAGTTAAAACAGCTAACCATTTTCCTAAATAAATCACTAGCCAGTTTCAAAAACTTGCTTAGGAACTTCATCATGTTTTAAGGTTCTTCCACCTCTTTTGCTAGGAACTAAGGTACTCCTCTGTTTTTGAACCCTATTATTATTGAATTTTTGTTTCTTTTTAGGTTTTTTTCGTTTCTTTTTACGTTTTCTTCTAGGTCTCTTTGGTTGAGACGGTTTGAAGCACTCTAAGTACTGAAGTTTTCTCTAATTTCTTCTTAGCTCTTTTCTTTGGTTCCTTCTTCTCATGACTCCGAGATTTCTTCTTCTCTTTCTTGCTATCAATGCTAATAGAAGCCTTTGAAAGATGTTGATAGTTTTTTATTAGTATCTTATACTAGCGATTGTACGACCGTTTCATTTTCATCAACAGAGCTTCGTTTTTGTCGAATATGTCAAGGAACAAGCCAATAAACTCTATTCTGTAGACTTCTTCTATTATTTTGACCCACGCTAATAATATATCTTTTTAAACTCGGGATGTAGTATTCATTGGTCAATAAACGTTGATCGTCATTCTTACATTGAAATAAGATAGATCCTTTTCATTGAATCGATACAATTATTGGTTTTAACTTTGATCAAAATATACCAAAACAAATAAATGGAAGAGAAAGAACAACTTGAGGGAAGAATTTCTTGGACTAAGAACCTTAAGCATTAAGCCTTTTAGTTCTTTTATGTCGGTTGTGAATAAAACATTTGAGTATTTATACGGGGGACAAGGCACTAACAAATTTTCTAAACTTTATCCTCAAGTTAAAACATGCTAATAATTCTCTTAAATAAATCACCAACTATTTTCCAAAACTTAGCTCTAGAATTTGGCAACTATTAACGGTTAGTTTACTACTAACAATTCTTTTACTAAGTCAAGATTATTGAATCACAAATGGATCTAAAATAAGTGATAGTTATACATAGTGTCCTATACAAAAATTAAATAATAAATTATGAGGTTTTACTATATAAATCACAACTTCTACTTCCATTATAGTTAGTGAGACATGAGTTCTAGAGCAGACTCTACATCCAACCTTTGTCTTAGAGAAGATCGAGATATGTCTTGTAATATGACATTTTATGTAACTCTTTAAACACTTTAACTCAAAGAGTTTAAATAGTCATTTTGGTCTCTAGCACTATACTTTTTCTCTGGAAATCATTCCTTATACATTGAACTTTCAAATTTCCTTATCAATTTGTATATTAATATTATCATAACAAAGAGTGGCAACCAGTGACAAACTGACAAATTAAATTGCGTTTACAATAGCAGATTATGCAATAAAAACATGTATGAAAGATAACATTAGAAATTACAATAAAATTTTATGTGATTTAAAAGAAATAAATGAACTCAGTAAGAACGTTTACAAGCACAGGTATGAGATTGTTTACAAGGTAGGAAACAAGATTGGGATACAAGAGTTATAAAATATGACATTCTATAACATGCAAACATACTAGATGGACACCCTCTATGGCTTCAAAGTTCCCGAGTACCTCTTGTCTCAACCTGTGACATGAGTCACTTGAGAAAAAATAAAGGAACGAAGGGGTGAGTATAAAATTATACTTAGCAAGCAACTTACTTGTGGGCTCTCATCACATCCATTGCCTAGTTGGTGTCTACAAACTTTTCTCTAGGCTCAAGGTATGCAACCTTTACTTCTCTCGGACTTAAGTAACTATCTTAGCCTAAGTAGTGGTTACTCTCGGGTTGAGACATTACCTCATAAAACTCTTTCACTAATGGAGACATTGTGGTATGTGTTATCTTGCTCTAATCTATGGGAGGTTAGTTGGCAAACCCTACCTACAGGACCATCATGTTATCCAAGGTTGGTGGAATGACCCCTTTGTGCTTGCTAGCAACTAGGACTACCTACATAATCCTACGTCCTCTCGAAGGATGGATCCGTTGTGTAGGGTAGTCCAACTAACTTGTCAGTCACACAACCCTAACAAATTTAGGCTTGACTTAACGGTATTGGTACTAGTATGGAGGAAAGTGGAAGTCTATTTGATACCATACCTACTGCTATAAGCTTGGATACTACCTTAGTCTATCTAGTTTGTTGTGGCAGTACTCTTAGGTTACTAAGGCGGGTTGGTGGCTTTGACATGGACTCTCCTCTCTATGATGATCTGTTCTGGCTCCTTGACCCTTACTTGGTTGAGTGAGTGTAGACCCATTGATCGCCACTCATGATTATAAGGTGCTCAGTCTGTTAGAGTCATTCGATCTCTACGAATCGTCTAGCCAATCTGTTTCTAATACCCTAAGCTTATCTCTTAATAACTAAATTATTTCTATTAGTGAGAATACACATTCATGCTCCTGAAGCTAATGTACAATAAGACATGCAATTCAACCACACTTACCAGGGTTATAACATCCATCAAATAACCTGAGCACATATCACAAGTTTTCATCATCTGTCTGCATATTTATGAACATATTAGTAATAAACCATCATGGACTTACGTGTTCGAAAACTTAAATTCCATCTAAAAATACAGTTATGGACTTACCCATTCTAAAATATCTTTAAAATGACGTAATAATAAACTAAATTAAACAAATAAACATTTAAATTAAAGACATCTCATTCTAACTCAAATCTAAGAAAAATAAATGTGACTACCCTACGTATGTGACATGGTCTCGAGTTGCGATTTCGTCATGAACCATATAGGAATTCCTCGCCATTACTTGAGATAAGAGTAGCATATGGCTTGAGTATTTTAAAACATACTTCGTAAGTGACCCCACTATTAAGGTCAAATGCAAACACATACAATCTCATCAATGAGACCTATCATAATTGTCTATTTTCTTACGACGGCTATCCGACCAGGCAATTGGGATGTGTACTTAATTTTCCTACACACAGTCTACACGTGCGAATACGAACCCACCAATCGGTTCGCACACCTCCTCGGCACCCTTTCTAGTCGAGCAAGCATTCTCCAGGAGTTGTTGCTGCGTAGCACCTGTTAGGAATAGCAACCACCACAGCAACATTATGATAAACATAATGAACGTTCCCCAGACTAGTAGTGTACGCGTCTGTACTATCGTCAAGGAATAGGGGTATCCCCTAATGGATGAACACACAATAATGCATGAGGTCCCAACATTTTTCTATTTTAATTATCATTAATACAACCCTGATAGCATCTCGTACTTATCATATCATTTCTCATATCATTTATCATAATTCTCATGTGTATGCCGGAGTTGTATTTTATACTAGTTCATCGTTTTGCATGTCAATCATAACATATCATTCATGTCACATATCAATCATATCATAACGTTTTTATGCTTTCAGACATAACAATCCATATCGTGCACATATTATATCATATTGTGTATCATAACAATCACATAACATCCCTAACATATCGGTTCACATTATATCATATCATAACCGTGTCAATAATTACATATACCGTACCATAACCTATATCATTTAAATCTCATCATTTTACTATGCATATATATTCGACAAATGGGTTAAGACTTCTAATAATAAAAGATATTTATGCATTGTTATTATTTATTCCAAGTTAAAACTTCTAATAGTAATAAAAGGGTTTAAAATGGAGAGATACCATTGCATAAAAATCTCCATTTATTTATAGCAGGTGCCATGAACAAAATTTAAGAGGATGATCTTGCAATTGGAACAGAATCTCTTCTGGTGCTGTCAAATGGGACAAAACCATTAAAACATACCATATTCATTTTGATAATATCCCTTATTGATTCAATGTTTTTAGAACAGTATGAGTTGTTAAATAATTTACATTCCTGCCATAATTATCTTAATAATTTCTTCAACAAACCATGAATTTCCACTATTCCCAACTCTATAAAGAGAAATGGAAGATGAAGCCCAAATGAAACACTGATCACCAAGGAATCATTATTCTTAAAGAGGAAAAGAAATGGAGGCCTTCAATCTTCCTCCTTTACTTCTGTCCTTTCTTTTTGTTCTTTTGCAAACATCCACCATTGCAGCCAAGAAGGTTAGTCATTCATCACTTATAAATCCTTTTTAATGTTTATACCAAATGCGTTTTAGACACTCAAGTTTTTTTCGAAGTTTTTTTCTTAATTGTGTGCAGTCTTATATTGTTTACTTGGGATCACATTCACATGGGTTGAATCCTTCCGCAATTGATCTTCAACGTGCAACACAAACCCACTACAATCTACTTGGATCCATGTTAGGAAGGTGAATCTTTTAGTTTCAAATCAGGTAGTGCAGATTTGTTGAACTCTCACTCCTAATTTACTATCGTATGTTTCTGTTTCCAAGTATAGCAACGAAGCAGCTAAGGAAGCAATCTTTTACTCATACAATAGACATATCAATGCCTTTGCAGCTGTTCTTGATCAGAAAGTTGCAGAAGATATAGCAAGTAAAATGATTCTATCTGTTTTGATAATGATTTTCTTCCTAGTAAAATTATATTTGTTTATTCCTAATTTATTGGGTTTGATAGTTTTATGGAAACATTACAAGACTCATAAAAAAAAGTTCCGAAAACCCTTTTTAGTTTCCAAAATTTTGTAGGTGGGAAGTATTCACAAGCTTAATTTCAAAAGTCAAATAGTAATCAAGGCGATTCTCGTTTCTTTAATTTAATAATGATGATGTTTTTATCCTTTTTCTTTTTTAGAGCATCATGATGTGGTATCAGTACATGAGAACAAAAAACTAAAACTGCACACTACACGATCATGGAACTTTCTTGGAGTTGAGAATGATGGTGGAATTCCTTTAGACTCACTTTGGAATCTTTCAAGATTTGGTGAATCTACAATCATTGGCAACATTGACTCTGGTTGGTCTTACTCATGTTTGAATTGTTTTTTTTTTTTTTTTGAGCTTTTGTAATAATTTTTATATGAAATTGAAATTCATACAGGTGTTTGGCCTGAATCAAAGAGTTTTAGTGACGAAGGATATGGACCTATCCCAACAAGATGGAAGGGAAGTTGTGAAGGTGGCACCCACTTTAGTTGCAATAGGTCCCTAACATTAAATCTCTTATTGTTACTTCATCTCTAATTAATTCTTTTAGAAATATGCACACTTTCAATTGTCTAATATGTTAGGCACAAATTAGACCCTTTCATTAGCACAATAGATAAATAATTGACAATAGTTGTTCAAAGTGAATATTAATCTAAAATTTGTTAGATCTCTATCGAAACATTATTAATGATACTAAAAAGAAATTTATAAATTTTTAGAGCAAAATTAAGTCAAAGTCATTTTCTTGGTTAAATTATAAGTATAGTTCATAAACTATCAAATTTATGTTTAACATATCTTTAAATTTTAAAAATTATATAATAAGGATTTGAACTTAACTCCTTTTGAATTTTCTTTTTTATATACAAATGATAATAGATTTTTCTTTGTCGCGTTTGTATTCTAGCTAATCATTGTATCAAATGCTTGTATACAATTTTAAATGTAGGAAGCTGATTGGAGTACGATATTTCAACAAAGGTTTTGCATCCGACGTGGGACCTCTCAACTCAAGCTATGAAACAGCAAGGGACGTTGATGGGCATGGAACACACACCTTATCCACGGCTGGAGGCAATTTCGTTAAAGGAGTAAGCATTTTAGGGAATAGTTATGGCACTGCAAAAGGGGGTTCCCCTAAAGCCCTTGTTGCTGCCTATAAAGTATGTTGGTCTACAGATAGAGGTGATGGGTGTTTTATGGCCGATATTCTAGCTAGCTTTGAAGCTGCCATTAGTGATGGAGTTGATGTTCTATCGGTTTCACTCGGTGGAGGTATTCAAGAATTTTCCGACGATCTTATAGCTATAGGGTCCTTCCATGCAGTGAAGAACGGCATCACTGTTGTTTGTTCAGCTGGAAATTCAGGACCAAGTGAAGATACTGTCTTAAATGTCGCACCATGGATGATAACTGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTACGTGGTGTTGGGAGACAAGAGGCAATTCAAGGTACTCTTTCTAACACTGGTTAGTAGTATATAGTATTGTCCTTATACATATTTAGGTAAGTATGGAACCAGTTTTAAATATAGTAAATTGCATTAGGCATGTGAAATTGTATGTATACGTGTCTATATTTGTATCCATTGAGTTTATGGCTCTCTACTATGTTTATGTTCTCTTCAATGATGTTTCATTATCAATACCAGGGTGAAAGTCTTTCTAGTAAATTATTGCCACCTAAGAAGTTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAACACCTCTAATCACGAAGCGTGAGTATTTTTTTCTCTAAACTATACAAAATATCCCTAAATTTTAGATACATTTCAATCATGTCTTAAACTTGAATTTTAAATTAGATCCAAAAATTTTCCAACAATAAAAATTAATCATTTTTTTTATATAGTGAGTTATGATCATAATTTTTTCTAATTTGTAGAATATTTGCATCTTTCACAATTTTTGAAAGTTTTAAAATAAAATTTTTCAAAATCGTTTAAGAGTATTTTATATAATTTAATCCCGATAGTTTTACAATAATAATTAGAATAAATTGATTATAGCACATGATACTAAGCAAAATTTATTGTGATCTTGTAGCATACTATGTCGTCAAGGGTCTCTTAATCCTGAAAAGGTAAGAGGAAAAATTGTAGTTTGCCTTAGAGGGGAAAATTCAAGAGGGGAGAAAGGTTACGTGGTTGCTCAAGCAGGTGGTGTTGGGATGATTCTCGCTAATGACAAAGAAAGTGGGGATGAACTTTCGGCTAGTCCTCACTTTCTTCCTGCTTCACATATAAGCTATACCGATGGTGAATCAGTCTACCAATATATCCAATCTACTAAGTAATTACGCCATCTTATTCCATCAACTTCCATATTTATTCACGAAGTATTTTGAACTTTTTGATCTAATAAACATAAAATTAGAAGCATAGGAATCTATTAAATGTATTTTTTAAGTTAAAAAATTTATCAAACTCAAAATTTGTTACTTATATTTGATGTTTGTGGTTGAAACAGCACTCCAATAGCTTACATGACTTCCGTGACTGAGTTGGGAATCAAACCAGCACCAATTATGGCTTCATTCTCTTCGAGAGGTCCCAACATAGTTGAGCCCTCAATACTCAAGGTTGGTTAAAAACGATGTGTTGAGTACTTGTATAGTATAAAGTTCAATGTGTCATCCCCTAACAATCACAAAATTTTAAATAATGCATTTTTTTAAAAAATTCAGCCTGATATAACAGCACCAGGGGTGAATATAATAGCGGCTTTCTCTGAAGCAACATCCATATCTGGTTTACCTTATGATAAGCGTCAAGCTCAATTTATTACATTATCTGGCACTTCCATGTCATGCCCCCATATTTCTGGCATTGTCGGCCTTCTCAAAACGCTTTATCCAAAATGGAGTCCAGCAGCTATCAGATCTGCAATCATGACGACAGGTTCATTATTCAAAATTCTTCTATCATTTTAAAAGTTAAGGGACTAATTAAACTAATAGTTTATATATAGTAAGAGATGTTATTTATGGTATTTGCATTATTTTGCATTAAGAAACTTTTGATTTAAATCTTGCTGACTAACGGTTTTGTTTGGTAGCCGAAACCGAAGCCAATGACTTAAATCCAATACTAACCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGTCATGTCCAACCAAACAAAGCATCAAATCCTGGCCTTGTTTACGACCTCACCACCCAAGACTACTTGAACTTCCTATGTGCCCGTGGCTACAATAAAACACTATTGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAACAGATTTAAACTACCCATCAATCTCAATGAATAATCTGAAATCAGAGGCAGTAGAGATCAAAAGAAGAGTAAAAAATGTGGGAAGTCCAGGCATGTATGTTGCTCAAGTCGAGGCACCACCAGGAGTTTCTGTTTCGGTAGACCCGAGTACTTTGAAGTTCACTAAAACTGATGAAGAGAAGGATTTCAAAGTTGTGCTAAGGAGGGTGCCAAATAATCAAACTGAACAGAATGTGTTCGGAAAACTTGTATGGTCTGATGGAAAACATCGTGTTAGTAGCCCGATTTTTGTGATATTAGGGATTTAGTAAAACAAGCACATGAGTTGGATTGAGTTTAGCTCAATCTTTTGAAAATTTATTTTCTGGTTAACAGTTCTTATGTCGCATAATAATGAATTTTTAGTTGTTTTTTTTTAGCTTAATTTATTTAAGTTTTTTTTAGTTTAATTTATTTAAGTTGTTTTTTATTTAAGTTTTCGTCATGTTGTAAGCTACTCCTCTTTTTCAACCCTATTATTATTGAATTTTTGTTTTTTAAGGTCGGCCTCTAGGCCTCTTTGGTTAAGACAGTAAGTTTTATGAAACACCTTGACTCGTAGATGGCTATTTCTGACTAAGGTGACCTCTAATTTCTTCTTAGCTATGTTCTTTCGTTCCTTACTCTCACGACTCCCCGATTTCTTCTTCTCTTTCTTGCCCTCGATTCCAATGGAGGTTTTTGGAAGATATTGATGGTATTTTATTAGTATCTTATACTGGCGATTTTGCGACTGTTTTGCCTTCTTCAACAGAGCTTCATTTCTGTCGAATATCTTGAAGAACGAGTCAACTAACTCCATTATTCTACCTTCTTTCTTCGGTTACTTGATCAAAGCTAATAATATTGTTTTTCAAACTTGGGATGTAATATTCCTTGGTCAATAGAAATAGACATTGATCGTGGTTCTTACACTGGAATCATATAGATCCTTTGCCTTTGATCGGTACAATTATTGGTTTTGACCTAAGATCAAAATATACCAAAACAAATAAATGAAGGAGAAAGAACAACTTGAGGAGAGAGTTTCTTGGACTAAGAACTTTAAACACTATGCTTTTTAGTTCTCTTTTGTCGTTGTGAATATAGCTTACATTTAATGAGTATTTATATATGGGAGAAAACACTAACAAATTTTCTAAACGTTTTCTTCCGGTTAAAACGTCTAATAATTCTCCTAAATAAATTATTAAATATTTTCCAAAATTTAGCTCCACAATTTGTCAACTACTAAAAATTGTTTTACAACTTACAGTTCTTTTACCAAGTCAAGTTTATTGACTCACATAACGATCTAAGGTAGGTGACAACTATAAAAAAAGTATCTTATACAAAATTTAAATAATAAATTACTGAGTTTTTATGACATAAATCCCAACAATCTTCCACGAGCATTCAACATATTTTTCTTAGGGAGGATGAGATGTCTTATTTTTATATAAATCAAAACAATTGTCATTAAATAGTCATTTTGGTCCATACCACTATATTTTCTCTGTCAAAATCATTCCGTATACATTTATCTTTCACCCTTTCCTTGAAATCAATTTGTACATTAATATTTTGATAAAGAGCGGCAAAGAGAGACAAATTAAATCAAATTTACTATACTGTCACGACTTGAAATTTGAGCTCGTAATAAAAACATGTATGAAAGATTACTTTGAAAATTAAAATTTTATGCGATTTAACAGAAATAA

mRNA sequence

ATGTATAATCTAAAATCAGAGGTAGTGAAGATTAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGGCACCACCAGGAGTTTTGGTTTCGGTAGACCCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGGATTTTCAAGTTGTGTTGAGGAGGGTTCAAAGTAATCAAACTGAAGAAAAGCATGTGTTCGAAAAACTTGTGTGGCCTGATGGAAAACATCGTTCTTATATTGTTTACTTGGGATCACATTCACATGGGTTGAATCCTTCCGCAATTGATCTTCAACGTGCAACACAAACCCACTACAATCTACTTGGATCCATGTTAGGAAGCAACGAAGCAGCTAAGGAAGCAATCTTTTACTCATACAATAGACATATCAATGCCTTTGCAGCTGTTCTTGATCAGAAAGTTGCAGAAGATATAGCAATACATGAGAACAAAAAACTAAAACTGCACACTACACGATCATGGAACTTTCTTGGAGTTGAGAATGATGGTGGAATTCCTTTAGACTCACTTTGGAATCTTTCAAGATTTGGTGAATCTACAATCATTGGCAACATTGACTCTGGTGTTTGGCCTGAATCAAAGAGTTTTAGTGACGAAGGATATGGACCTATCCCAACAAGATGGAAGGGAAGTTGTGAAGGTGGCACCCACTTTAGTTGCAATAGGAAGCTGATTGGAGTACGATATTTCAACAAAGGTTTTGCATCCGACGTGGGACCTCTCAACTCAAGCTATGAAACAGCAAGGGACGTTGATGGGCATGGAACACACACCTTATCCACGGCTGGAGGCAATTTCGTTAAAGGAGTAAGCATTTTAGGGAATAGTTATGGCACTGCAAAAGGGGGTTCCCCTAAAGCCCTTGTTGCTGCCTATAAAGTATGTTGGTCTACAGATAGAGGTGATGGGTGTTTTATGGCCGATATTCTAGCTAGCTTTGAAGCTGCCATTAGTGATGGAGTTGATGTTCTATCGGTTTCACTCGGTGGAGGTATTCAAGAATTTTCCGACGATCTTATAGCTATAGGGTCCTTCCATGCAGTGAAGAACGGCATCACTGTTGTTTGTTCAGCTGGAAATTCAGGACCAAGTGAAGATACTGTCTTAAATGTCGCACCATGGATGATAACTGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTACGTGGGTGAAAGTCTTTCTAGTAAATTATTGCCACCTAAGAAGTTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAACACCTCTAATCACGAAGCCATACTATGTCGTCAAGGGTCTCTTAATCCTGAAAAGGTAAGAGGAAAAATTGTAGTTTGCCTTAGAGGGGAAAATTCAAGAGGGGAGAAAGGTTACGTGGTTGCTCAAGCAGGTGGTGTTGGGATGATTCTCGCTAATGACAAAGAAAGTGGGGATGAACTTTCGGCTAGTCCTCACTTTCTTCCTGCTTCACATATAAGCTATACCGATGCTTACATGACTTCCGTGACTGAGTTGGGAATCAAACCAGCACCAATTATGGCTTCATTCTCTTCGAGAGGTCCCAACATAGTTGAGCCCTCAATACTCAAGCCTGATATAACAGCACCAGGGGTGAATATAATAGCGGCTTTCTCTGAAGCAACATCCATATCTGGTTTACCTTATGATAAGCGTCAAGCTCAATTTATTACATTATCTGGCACTTCCATGTCATGCCCCCATATTTCTGGCATTGTCGGCCTTCTCAAAACGCTTTATCCAAAATGGAGTCCAGCAGCTATCAGATCTGCAATCATGACGACAGCCGAAACCGAAGCCAATGACTTAAATCCAATACTAACCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGTCATGTCCAACCAAACAAAGCATCAAATCCTGGCCTTGTTTACGACCTCACCACCCAAGACTACTTGAACTTCCTATGTGCCCGTGGCTACAATAAAACACTATTGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAACAGATTTAAACTACCCATCAATCTCAATGAATAATCTGAAATCAGAGGCAGTAGAGATCAAAAGAAGAGTAAAAAATGTGGGAAGTCCAGGCATGTATGTTGCTCAAGTCGAGGCACCACCAGGAGTTTCTGTTTCGGTAGACCCGAGTACTTTGAAGTTCACTAAAACTGATGAAGAGAAGGATTTCAAAGTTGTGCTAAGGAGGGTGCCAAATAATCAAACTGAACAGAATGTGTTCGGAAAACTTAAATAA

Coding sequence (CDS)

ATGTATAATCTAAAATCAGAGGTAGTGAAGATTAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGGCACCACCAGGAGTTTTGGTTTCGGTAGACCCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGGATTTTCAAGTTGTGTTGAGGAGGGTTCAAAGTAATCAAACTGAAGAAAAGCATGTGTTCGAAAAACTTGTGTGGCCTGATGGAAAACATCGTTCTTATATTGTTTACTTGGGATCACATTCACATGGGTTGAATCCTTCCGCAATTGATCTTCAACGTGCAACACAAACCCACTACAATCTACTTGGATCCATGTTAGGAAGCAACGAAGCAGCTAAGGAAGCAATCTTTTACTCATACAATAGACATATCAATGCCTTTGCAGCTGTTCTTGATCAGAAAGTTGCAGAAGATATAGCAATACATGAGAACAAAAAACTAAAACTGCACACTACACGATCATGGAACTTTCTTGGAGTTGAGAATGATGGTGGAATTCCTTTAGACTCACTTTGGAATCTTTCAAGATTTGGTGAATCTACAATCATTGGCAACATTGACTCTGGTGTTTGGCCTGAATCAAAGAGTTTTAGTGACGAAGGATATGGACCTATCCCAACAAGATGGAAGGGAAGTTGTGAAGGTGGCACCCACTTTAGTTGCAATAGGAAGCTGATTGGAGTACGATATTTCAACAAAGGTTTTGCATCCGACGTGGGACCTCTCAACTCAAGCTATGAAACAGCAAGGGACGTTGATGGGCATGGAACACACACCTTATCCACGGCTGGAGGCAATTTCGTTAAAGGAGTAAGCATTTTAGGGAATAGTTATGGCACTGCAAAAGGGGGTTCCCCTAAAGCCCTTGTTGCTGCCTATAAAGTATGTTGGTCTACAGATAGAGGTGATGGGTGTTTTATGGCCGATATTCTAGCTAGCTTTGAAGCTGCCATTAGTGATGGAGTTGATGTTCTATCGGTTTCACTCGGTGGAGGTATTCAAGAATTTTCCGACGATCTTATAGCTATAGGGTCCTTCCATGCAGTGAAGAACGGCATCACTGTTGTTTGTTCAGCTGGAAATTCAGGACCAAGTGAAGATACTGTCTTAAATGTCGCACCATGGATGATAACTGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTACGTGGGTGAAAGTCTTTCTAGTAAATTATTGCCACCTAAGAAGTTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAACACCTCTAATCACGAAGCCATACTATGTCGTCAAGGGTCTCTTAATCCTGAAAAGGTAAGAGGAAAAATTGTAGTTTGCCTTAGAGGGGAAAATTCAAGAGGGGAGAAAGGTTACGTGGTTGCTCAAGCAGGTGGTGTTGGGATGATTCTCGCTAATGACAAAGAAAGTGGGGATGAACTTTCGGCTAGTCCTCACTTTCTTCCTGCTTCACATATAAGCTATACCGATGCTTACATGACTTCCGTGACTGAGTTGGGAATCAAACCAGCACCAATTATGGCTTCATTCTCTTCGAGAGGTCCCAACATAGTTGAGCCCTCAATACTCAAGCCTGATATAACAGCACCAGGGGTGAATATAATAGCGGCTTTCTCTGAAGCAACATCCATATCTGGTTTACCTTATGATAAGCGTCAAGCTCAATTTATTACATTATCTGGCACTTCCATGTCATGCCCCCATATTTCTGGCATTGTCGGCCTTCTCAAAACGCTTTATCCAAAATGGAGTCCAGCAGCTATCAGATCTGCAATCATGACGACAGCCGAAACCGAAGCCAATGACTTAAATCCAATACTAACCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGTCATGTCCAACCAAACAAAGCATCAAATCCTGGCCTTGTTTACGACCTCACCACCCAAGACTACTTGAACTTCCTATGTGCCCGTGGCTACAATAAAACACTATTGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAACAGATTTAAACTACCCATCAATCTCAATGAATAATCTGAAATCAGAGGCAGTAGAGATCAAAAGAAGAGTAAAAAATGTGGGAAGTCCAGGCATGTATGTTGCTCAAGTCGAGGCACCACCAGGAGTTTCTGTTTCGGTAGACCCGAGTACTTTGAAGTTCACTAAAACTGATGAAGAGAAGGATTTCAAAGTTGTGCTAAGGAGGGTGCCAAATAATCAAACTGAACAGAATGTGTTCGGAAAACTTAAATAA

Protein sequence

MYNLKSEVVKIKRRVKNVGSPGTYVAKVEAPPGVLVSVDPSTLKFTKTGEEKDFQVVLRRVQSNQTEEKHVFEKLVWPDGKHRSYIVYLGSHSHGLNPSAIDLQRATQTHYNLLGSMLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIAIHENKKLKLHTTRSWNFLGVENDGGIPLDSLWNLSRFGESTIIGNIDSGVWPESKSFSDEGYGPIPTRWKGSCEGGTHFSCNRKLIGVRYFNKGFASDVGPLNSSYETARDVDGHGTHTLSTAGGNFVKGVSILGNSYGTAKGGSPKALVAAYKVCWSTDRGDGCFMADILASFEAAISDGVDVLSVSLGGGIQEFSDDLIAIGSFHAVKNGITVVCSAGNSGPSEDTVLNVAPWMITVGASTVDRLFTSYVGESLSSKLLPPKKFYPLIRALDAKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLRGENSRGEKGYVVAQAGGVGMILANDKESGDELSASPHFLPASHISYTDAYMTSVTELGIKPAPIMASFSSRGPNIVEPSILKPDITAPGVNIIAAFSEATSISGLPYDKRQAQFITLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETEANDLNPILTSEKEKANPLAYGAGHVQPNKASNPGLVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFVCSKSFKVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPGMYVAQVEAPPGVSVSVDPSTLKFTKTDEEKDFKVVLRRVPNNQTEQNVFGKLK
Homology
BLAST of CmaCh16G010020 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 2.9e-225
Identity = 404/739 (54.67%), Postives = 510/739 (69.01%), Query Frame = 0

Query: 70  HVFEKLVWPDGKHRSYIVYLGSHSHGLNPSAIDLQRATQTHYNLLGSMLGSNEAAKEAIF 129
           H+  K +       SY+VY G+HSH    +   + R  +THY+ LGS  GS E A +AIF
Sbjct: 17  HMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIF 76

Query: 130 YSYNRHINAFAAVLDQKVAEDIAIH-------ENKKLKLHTTRSWNFLGVENDGGIPLDS 189
           YSY +HIN FAA LD  +A +I+ H        NK LKLHTTRSW+FLG+E++  +P  S
Sbjct: 77  YSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSS 136

Query: 190 LWNLSRFGESTIIGNIDSGVWPESKSFSDEGYGPIPTRWKGSCEG--GTHFSCNRKLIGV 249
           +W  +RFGE TII N+D+GVWPESKSF DEG GPIP+RWKG C+      F CNRKLIG 
Sbjct: 137 IWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGA 196

Query: 250 RYFNKGFASDVGPLNSSYETARDVDGHGTHTLSTAGGNFVKGVSILGNSYGTAKGGSPKA 309
           RYFNKG+A+ VG LNSS+++ RD+DGHG+HTLSTA G+FV GVSI G   GTAKGGSP+A
Sbjct: 197 RYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRA 256

Query: 310 LVAAYKVCWSTDRGDGCFMADILASFEAAISDGVDVLSVSLGGGIQEFSDDLIAIGSFHA 369
            VAAYKVCW   +G+ C+ AD+LA+F+AAI DG DV+SVSLGG    F +D +AIGSFHA
Sbjct: 257 RVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHA 316

Query: 370 VKNGITVVCSAGNSGPSEDTVLNVAPWMITVGASTVDRLFTS---------YVGESLSSK 429
            K  I VVCSAGNSGP++ TV NVAPW ITVGAST+DR F S         Y G+SLSS 
Sbjct: 317 AKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSST 376

Query: 430 LLPPKKFYPLIRALDAKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLRGENSRGEKGYVV 489
            LP  KFYP++ +++AK+ N S  +A LC+ GSL+P K +GKI+VCLRG+N R EKG  V
Sbjct: 377 ALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAV 436

Query: 490 AQAGGVGMILANDKESGDELSASPHFLPASHISYTDAYMT----------------SVTE 549
           A  GG+GM+L N   +G++L A PH LPA+ ++  D++                  S T+
Sbjct: 437 ALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTD 496

Query: 550 LGIKPAPIMASFSSRGPNIVEPSILKPDITAPGVNIIAAFSEATSISGLPYDKRQAQFIT 609
           LG+KPAP+MASFSS+GP+IV P ILKPDITAPGV++IAA++ A S +   +D R+  F  
Sbjct: 497 LGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNA 556

Query: 610 LSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETEANDLNPILTSEKEKANPLA 669
           +SGTSMSCPHISGI GLLKT YP WSPAAIRSAIMTTA    +   PI  +   KA P +
Sbjct: 557 ISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFS 616

Query: 670 YGAGHVQPNKASNPGLVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFVCSKSFKVTDLN 729
           +GAGHVQPN A NPGLVYDL  +DYLNFLC+ GYN + + +F+ +     S    + +LN
Sbjct: 617 FGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLN 676

Query: 730 YPSISMNNLKSEAVEIKRRVKNVGSPGMYVAQVEAPPGVSVSVDPSTLKFTKTDEEKDFK 775
           YPSI++ NL S  V + R VKNVG P MY  +V  P GV V+V P++L FTK  E+K FK
Sbjct: 677 YPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFK 736

BLAST of CmaCh16G010020 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 4.6e-223
Identity = 411/724 (56.77%), Postives = 503/724 (69.48%), Query Frame = 0

Query: 83  RSYIVYLGSHSHGLNPSAIDLQRATQTHYNLLGSMLGSNEAAKEAIFYSYNRHINAFAAV 142
           +SYIVYLGSH+H    S+  L     +H   L S +GS+E AKEAIFYSY RHIN FAA+
Sbjct: 40  KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAI 99

Query: 143 LDQKVAEDIAIH-------ENKKLKLHTTRSWNFLGVENDGGIPLDSLWNLSRFGESTII 202
           LD+  A +IA H        NK  KLHTT SWNF+ +  +G +   SLWN + +GE TII
Sbjct: 100 LDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTII 159

Query: 203 GNIDSGVWPESKSFSDEGYGPIPTRWKGSCEGGTHFSCNRKLIGVRYFNKGFASDVG-PL 262
            N+D+GVWPESKSFSDEGYG +P RWKG C       CNRKLIG RYFNKG+ +  G P 
Sbjct: 160 ANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPS 219

Query: 263 NSSYETARDVDGHGTHTLSTAGGNFVKGVSILGNSYGTAKGGSPKALVAAYKVCWSTDRG 322
           N+SYET RD DGHG+HTLSTA GNFV G ++ G   GTA GGSPKA VAAYKVCW    G
Sbjct: 220 NASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDG 279

Query: 323 DGCFMADILASFEAAISDGVDVLSVSLGGGIQEFSDDLIAIGSFHAVKNGITVVCSAGNS 382
             CF ADILA+ EAAI DGVDVLS S+GG   ++  D IAIGSFHAVKNG+TVVCSAGNS
Sbjct: 280 AECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNS 339

Query: 383 GPSEDTVLNVAPWMITVGASTVDRLFTSYV----GESLS----SKLLPPKKFYPLIRALD 442
           GP   TV NVAPW+ITVGAS++DR F ++V    G+S      SK LP +K Y LI A D
Sbjct: 340 GPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPEEKMYSLISAAD 399

Query: 443 AKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLRGENSRGEKGYVVAQAGGVGMILANDKE 502
           A   N +  +A+LC++GSL+P+KV+GKI+VCLRG+N+R +KG   A AG  GM+L NDK 
Sbjct: 400 ANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKA 459

Query: 503 SGDELSASPHFLPASHISYTD-----AYMTSVTE-----------LGIKPAPIMASFSSR 562
           SG+E+ +  H LPAS I Y D     +Y++S  +           L  KPAP MASFSSR
Sbjct: 460 SGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSR 519

Query: 563 GPNIVEPSILKPDITAPGVNIIAAFSEATSISGLPYDKRQAQFITLSGTSMSCPHISGIV 622
           GPN + P ILKPDITAPGVNIIAAF+EAT  + L  D R+  F T SGTSMSCPHISG+V
Sbjct: 520 GPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVV 579

Query: 623 GLLKTLYPKWSPAAIRSAIMTTAETEANDLNPILTSEKEKANPLAYGAGHVQPNKASNPG 682
           GLLKTL+P WSPAAIRSAIMTT+ T  N   P++    +KANP +YG+GHVQPNKA++PG
Sbjct: 580 GLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPG 639

Query: 683 LVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFVCSKSFKVTDLNYPSISMNNLKSEAVE 742
           LVYDLTT DYL+FLCA GYN T+++LF  D  + C +   + D NYPSI++ NL + ++ 
Sbjct: 640 LVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNL-TGSIT 699

Query: 743 IKRRVKNVGSPGMYVAQVEAPPGVSVSVDPSTLKFTKTDEEKDFKVVLRRVPNNQTEQNV 775
           + R++KNVG P  Y A+   P GV VSV+P  L F KT E K F++ LR +P   +   V
Sbjct: 700 VTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGY-V 759

BLAST of CmaCh16G010020 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 666.0 bits (1717), Expect = 5.2e-190
Identity = 374/751 (49.80%), Postives = 475/751 (63.25%), Query Frame = 0

Query: 79  DGKHRSYIVYLGSHSHGLNPSAIDLQRATQTHYNLLGSMLGSNEAAKEAIFYSYNRHINA 138
           +G  + YIVY+G+HSHG +P++ DL+ AT +HY+LLGS+ GS E AKEAI YSYNRHIN 
Sbjct: 26  NGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHING 85

Query: 139 FAAVLDQKVAEDIAIHEN-------KKLKLHTTRSWNFLGVENDGGIPLDSLWNLSRFGE 198
           FAA+L+++ A DIA + N       K+ KLHTTRSW FLG+   G    +S W   RFGE
Sbjct: 86  FAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QNSAWQKGRFGE 145

Query: 199 STIIGNIDSGVWPESKSFSDEGYGPIPTRWKGS-CE-----GGTHFSCNRKLIGVRYFNK 258
           +TIIGNID+GVWPES+SFSD+GYG +P++W+G  C+     G    +CNRKLIG RY+NK
Sbjct: 146 NTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNK 205

Query: 259 GFASDVGPLNSSYETARDVDGHGTHTLSTAGGNFVKGVSILGNSYGTAKGGSPKALVAAY 318
            F +  G L+    TARD  GHGTHTLSTAGGNFV G  +     GTAKGGSP+A VAAY
Sbjct: 206 AFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAY 265

Query: 319 KVCWSTDRGDGCFMADILASFEAAISDGVDVLSVSLGGG----IQEFSDDLIAIGSFHAV 378
           KVCWS      C+ AD+LA+ + AI DGVDV++VS G       +    D I+IG+FHA+
Sbjct: 266 KVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAI 325

Query: 379 KNGITVVCSAGNSGPSEDTVLNVAPWMITVGASTVDRLFTSYV--------GESLSSKLL 438
              I +V SAGN GP+  TV NVAPW+ T+ AST+DR F+S +        G SL    L
Sbjct: 326 SKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINNQLIEGASLFVN-L 385

Query: 439 PPKKFYPLIRALDAKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLR-GENSRGEKGYVVA 498
           PP + + LI + DAK  N +  +A LCR+G+L+  KV GKIV+C R G+     +G    
Sbjct: 386 PPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEAL 445

Query: 499 QAGGVGMILANDKESGDELSASPHFL------PASHISYTDAYMTSV------------- 558
            AG  GMIL N  ++G  LSA PH        P    S      T+              
Sbjct: 446 TAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDT 505

Query: 559 -------TELGIKPAPIMASFSSRGPNIVEPSILKPDITAPGVNIIAAFSEATSISGLPY 618
                  T  G KPAP+MASFSSRGPN ++PSILKPD+TAPGVNI+AA+SE  S S L  
Sbjct: 506 IKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLV 565

Query: 619 DKRQA-QFITLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETEANDLNPILT 678
           D R+  +F  L GTSMSCPH SGI GLLKT +P WSPAAI+SAIMTTA T  N   PI  
Sbjct: 566 DNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQD 625

Query: 679 S-EKEKANPLAYGAGHVQPNKASNPGLVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFV 738
           + +K  A+  AYG+GHV+P+ A  PGLVYDL+  DYLNFLCA GY++ L+     + +F+
Sbjct: 626 AFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFI 685

Query: 739 CSKSFKVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPGMYVAQVEAPPGVSVSVDPSTLK 776
           CS S  V DLNYPSI++ NL+ + V I R V NVG P  Y     +P G S++V P +L 
Sbjct: 686 CSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLT 745

BLAST of CmaCh16G010020 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.4e-158
Identity = 327/733 (44.61%), Postives = 453/733 (61.80%), Query Frame = 0

Query: 84  SYIVYLGSHSHGLNPSAIDLQRATQTHYNLLGSMLGSNEAAKEAIFYSYNRHINAFAAVL 143
           +YIV++        PS+ DL      H N   S L S   + E + Y+Y   I+ F+  L
Sbjct: 31  TYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRL 90

Query: 144 DQKVAED-------IAIHENKKLKLHTTRSWNFLGVENDGGIPLDSLWNLSRFGESTIIG 203
            Q+ A+        I++    + +LHTTR+  FLG++         L+  +      ++G
Sbjct: 91  TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA----DLFPEAGSYSDVVVG 150

Query: 204 NIDSGVWPESKSFSDEGYGPIPTRWKGSCEGGTHFS---CNRKLIGVRYFNKGFASDVGP 263
            +D+GVWPESKS+SDEG+GPIP+ WKG CE GT+F+   CNRKLIG R+F +G+ S +GP
Sbjct: 151 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 210

Query: 264 LNSSYE--TARDVDGHGTHTLSTAGGNFVKGVSILGNSYGTAKGGSPKALVAAYKVCWST 323
           ++ S E  + RD DGHGTHT STA G+ V+G S+LG + GTA+G +P+A VA YKVCW  
Sbjct: 211 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL- 270

Query: 324 DRGDGCFMADILASFEAAISDGVDVLSVSLGGGIQEFSDDLIAIGSFHAVKNGITVVCSA 383
               GCF +DILA+ + AI+D V+VLS+SLGGG+ ++  D +AIG+F A++ GI V CSA
Sbjct: 271 ---GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSA 330

Query: 384 GNSGPSEDTVLNVAPWMITVGASTVDRLF---------TSYVGESLSSKLLPPKKFYPLI 443
           GN+GPS  ++ NVAPW+ TVGA T+DR F          ++ G SL      P K  P I
Sbjct: 331 GNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFI 390

Query: 444 RALDAKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLRGENSRGEKGYVVAQAGGVGMILA 503
            A +A +    N    LC  G+L PEKV+GKIV+C RG N+R +KG VV  AGGVGMILA
Sbjct: 391 YAGNASNATNGN----LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILA 450

Query: 504 NDKESGDELSASPHFLPAS-----------HISYTDAYMTSV-----TELGIKPAPIMAS 563
           N   +G+EL A  H LPA+           H   TD   T+      T +G+KP+P++A+
Sbjct: 451 NTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAA 510

Query: 564 FSSRGPNIVEPSILKPDITAPGVNIIAAFSEATSISGLPYDKRQAQFITLSGTSMSCPHI 623
           FSSRGPN + P+ILKPD+ APGVNI+AA++ A   +GL  D R+ +F  +SGTSMSCPH+
Sbjct: 511 FSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHV 570

Query: 624 SGIVGLLKTLYPKWSPAAIRSAIMTTAETEANDLNPILTSEKEK-ANPLAYGAGHVQPNK 683
           SG+  LLK+++P+WSPAAIRSA+MTTA     D  P+L     K + P  +GAGHV P  
Sbjct: 571 SGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTT 630

Query: 684 ASNPGLVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFVC--SKSFKVTDLNYPSISMNN 743
           A+NPGL+YDLTT+DYL FLCA  Y    ++  +   ++ C  SKS+ V DLNYPS ++N 
Sbjct: 631 ATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSFAVNV 690

Query: 744 LKSEAVEIKRRVKNVGSPGMYVAQVEA-PPGVSVSVDPSTLKFTKTDEEKDFKVVLRRVP 776
               A +  R V +VG  G Y  +V +   GV +SV+P+ L F + +E+K + V      
Sbjct: 691 DGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDS 740

BLAST of CmaCh16G010020 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 2.3e-145
Identity = 303/684 (44.30%), Postives = 410/684 (59.94%), Query Frame = 0

Query: 109 THYNLLGSMLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIA--------IHENKKLK 168
           TH++   S L S    + ++ Y+Y    + F+A LD   A+ +         I E+    
Sbjct: 45  THHDWYTSQLNS----ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYT 104

Query: 169 LHTTRSWNFLGVENDGGIPLDSLWNLSRFGESTIIGNIDSGVWPESKSFSDEGYGPIPTR 228
           LHTTR+  FLG+ ++ G+      +L       IIG +D+GVWPES+SF D     IP++
Sbjct: 105 LHTTRTPEFLGLNSEFGV-----HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSK 164

Query: 229 WKGSCEGGTHFS---CNRKLIGVRYFNKGF-ASDVGPLNSSYETA--RDVDGHGTHTLST 288
           WKG CE G+ F    CN+KLIG R F+KGF  +  G  +S  E+   RDVDGHGTHT +T
Sbjct: 165 WKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTT 224

Query: 289 AGGNFVKGVSILGNSYGTAKGGSPKALVAAYKVCWSTDRGDGCFMADILASFEAAISDGV 348
           A G+ V+  S LG + GTA+G + +A VA YKVCWST    GCF +DILA+ + AI DGV
Sbjct: 225 AAGSAVRNASFLGYAAGTARGMATRARVATYKVCWST----GCFGSDILAAMDRAILDGV 284

Query: 349 DVLSVSLGGGIQEFSDDLIAIGSFHAVKNGITVVCSAGNSGPSEDTVLNVAPWMITVGAS 408
           DVLS+SLGGG   +  D IAIG+F A++ G+ V CSAGNSGP+  +V NVAPW++TVGA 
Sbjct: 285 DVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAG 344

Query: 409 TVDRLFTSYVGESLSSKLLPPKKFYPL---IRALDAKSNNTSNHEAILCRQGSLNPEKVR 468
           T+DR F ++       +L     +  +    + L+   N  ++  + LC  GSL+   VR
Sbjct: 345 TLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVR 404

Query: 469 GKIVVCLRGENSRGEKGYVVAQAGGVGMILANDKESGDELSASPHFLPASHIS------- 528
           GKIVVC RG N+R EKG VV  AGG+GMI+AN   SG+EL A  H LPA  +        
Sbjct: 405 GKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLL 464

Query: 529 ----YTDAYMTSV-----TELGIKPAPIMASFSSRGPNIVEPSILKPDITAPGVNIIAAF 588
                +D+  T++     T L +KP+P++A+FSSRGPN V P ILKPD+  PGVNI+A +
Sbjct: 465 REYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGW 524

Query: 589 SEATSISGLPYDKRQAQFITLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAET 648
           S+A   +GL  D R+ QF  +SGTSMSCPHISG+ GLLK  +P+WSP+AI+SA+MTTA  
Sbjct: 525 SDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYV 584

Query: 649 EANDLNPIL-TSEKEKANPLAYGAGHVQPNKASNPGLVYDLTTQDYLNFLCARGYNKTLL 708
             N   P+   ++   +NP A+G+GHV P KA +PGLVYD++T++Y+ FLC+  Y    +
Sbjct: 585 LDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHI 644

Query: 709 KLFTNDTSFVCSKSFK-VTDLNYPSISMNNLKSEAVEIKRRVKNVG-SPGMYVAQVEAPP 757
                  S  CSK F     LNYPS S+       V   R V NVG +  +Y   V   P
Sbjct: 645 VAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAP 704

BLAST of CmaCh16G010020 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 783.1 bits (2021), Expect = 2.1e-226
Identity = 404/739 (54.67%), Postives = 510/739 (69.01%), Query Frame = 0

Query: 70  HVFEKLVWPDGKHRSYIVYLGSHSHGLNPSAIDLQRATQTHYNLLGSMLGSNEAAKEAIF 129
           H+  K +       SY+VY G+HSH    +   + R  +THY+ LGS  GS E A +AIF
Sbjct: 17  HMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIF 76

Query: 130 YSYNRHINAFAAVLDQKVAEDIAIH-------ENKKLKLHTTRSWNFLGVENDGGIPLDS 189
           YSY +HIN FAA LD  +A +I+ H        NK LKLHTTRSW+FLG+E++  +P  S
Sbjct: 77  YSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSS 136

Query: 190 LWNLSRFGESTIIGNIDSGVWPESKSFSDEGYGPIPTRWKGSCEG--GTHFSCNRKLIGV 249
           +W  +RFGE TII N+D+GVWPESKSF DEG GPIP+RWKG C+      F CNRKLIG 
Sbjct: 137 IWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGA 196

Query: 250 RYFNKGFASDVGPLNSSYETARDVDGHGTHTLSTAGGNFVKGVSILGNSYGTAKGGSPKA 309
           RYFNKG+A+ VG LNSS+++ RD+DGHG+HTLSTA G+FV GVSI G   GTAKGGSP+A
Sbjct: 197 RYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRA 256

Query: 310 LVAAYKVCWSTDRGDGCFMADILASFEAAISDGVDVLSVSLGGGIQEFSDDLIAIGSFHA 369
            VAAYKVCW   +G+ C+ AD+LA+F+AAI DG DV+SVSLGG    F +D +AIGSFHA
Sbjct: 257 RVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHA 316

Query: 370 VKNGITVVCSAGNSGPSEDTVLNVAPWMITVGASTVDRLFTS---------YVGESLSSK 429
            K  I VVCSAGNSGP++ TV NVAPW ITVGAST+DR F S         Y G+SLSS 
Sbjct: 317 AKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSST 376

Query: 430 LLPPKKFYPLIRALDAKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLRGENSRGEKGYVV 489
            LP  KFYP++ +++AK+ N S  +A LC+ GSL+P K +GKI+VCLRG+N R EKG  V
Sbjct: 377 ALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAV 436

Query: 490 AQAGGVGMILANDKESGDELSASPHFLPASHISYTDAYMT----------------SVTE 549
           A  GG+GM+L N   +G++L A PH LPA+ ++  D++                  S T+
Sbjct: 437 ALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTD 496

Query: 550 LGIKPAPIMASFSSRGPNIVEPSILKPDITAPGVNIIAAFSEATSISGLPYDKRQAQFIT 609
           LG+KPAP+MASFSS+GP+IV P ILKPDITAPGV++IAA++ A S +   +D R+  F  
Sbjct: 497 LGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNA 556

Query: 610 LSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETEANDLNPILTSEKEKANPLA 669
           +SGTSMSCPHISGI GLLKT YP WSPAAIRSAIMTTA    +   PI  +   KA P +
Sbjct: 557 ISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFS 616

Query: 670 YGAGHVQPNKASNPGLVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFVCSKSFKVTDLN 729
           +GAGHVQPN A NPGLVYDL  +DYLNFLC+ GYN + + +F+ +     S    + +LN
Sbjct: 617 FGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLN 676

Query: 730 YPSISMNNLKSEAVEIKRRVKNVGSPGMYVAQVEAPPGVSVSVDPSTLKFTKTDEEKDFK 775
           YPSI++ NL S  V + R VKNVG P MY  +V  P GV V+V P++L FTK  E+K FK
Sbjct: 677 YPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFK 736

BLAST of CmaCh16G010020 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 775.8 bits (2002), Expect = 3.3e-224
Identity = 411/724 (56.77%), Postives = 503/724 (69.48%), Query Frame = 0

Query: 83  RSYIVYLGSHSHGLNPSAIDLQRATQTHYNLLGSMLGSNEAAKEAIFYSYNRHINAFAAV 142
           +SYIVYLGSH+H    S+  L     +H   L S +GS+E AKEAIFYSY RHIN FAA+
Sbjct: 40  KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAI 99

Query: 143 LDQKVAEDIAIH-------ENKKLKLHTTRSWNFLGVENDGGIPLDSLWNLSRFGESTII 202
           LD+  A +IA H        NK  KLHTT SWNF+ +  +G +   SLWN + +GE TII
Sbjct: 100 LDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTII 159

Query: 203 GNIDSGVWPESKSFSDEGYGPIPTRWKGSCEGGTHFSCNRKLIGVRYFNKGFASDVG-PL 262
            N+D+GVWPESKSFSDEGYG +P RWKG C       CNRKLIG RYFNKG+ +  G P 
Sbjct: 160 ANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPS 219

Query: 263 NSSYETARDVDGHGTHTLSTAGGNFVKGVSILGNSYGTAKGGSPKALVAAYKVCWSTDRG 322
           N+SYET RD DGHG+HTLSTA GNFV G ++ G   GTA GGSPKA VAAYKVCW    G
Sbjct: 220 NASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDG 279

Query: 323 DGCFMADILASFEAAISDGVDVLSVSLGGGIQEFSDDLIAIGSFHAVKNGITVVCSAGNS 382
             CF ADILA+ EAAI DGVDVLS S+GG   ++  D IAIGSFHAVKNG+TVVCSAGNS
Sbjct: 280 AECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNS 339

Query: 383 GPSEDTVLNVAPWMITVGASTVDRLFTSYV----GESLS----SKLLPPKKFYPLIRALD 442
           GP   TV NVAPW+ITVGAS++DR F ++V    G+S      SK LP +K Y LI A D
Sbjct: 340 GPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPEEKMYSLISAAD 399

Query: 443 AKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLRGENSRGEKGYVVAQAGGVGMILANDKE 502
           A   N +  +A+LC++GSL+P+KV+GKI+VCLRG+N+R +KG   A AG  GM+L NDK 
Sbjct: 400 ANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKA 459

Query: 503 SGDELSASPHFLPASHISYTD-----AYMTSVTE-----------LGIKPAPIMASFSSR 562
           SG+E+ +  H LPAS I Y D     +Y++S  +           L  KPAP MASFSSR
Sbjct: 460 SGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSR 519

Query: 563 GPNIVEPSILKPDITAPGVNIIAAFSEATSISGLPYDKRQAQFITLSGTSMSCPHISGIV 622
           GPN + P ILKPDITAPGVNIIAAF+EAT  + L  D R+  F T SGTSMSCPHISG+V
Sbjct: 520 GPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVV 579

Query: 623 GLLKTLYPKWSPAAIRSAIMTTAETEANDLNPILTSEKEKANPLAYGAGHVQPNKASNPG 682
           GLLKTL+P WSPAAIRSAIMTT+ T  N   P++    +KANP +YG+GHVQPNKA++PG
Sbjct: 580 GLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPG 639

Query: 683 LVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFVCSKSFKVTDLNYPSISMNNLKSEAVE 742
           LVYDLTT DYL+FLCA GYN T+++LF  D  + C +   + D NYPSI++ NL + ++ 
Sbjct: 640 LVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNL-TGSIT 699

Query: 743 IKRRVKNVGSPGMYVAQVEAPPGVSVSVDPSTLKFTKTDEEKDFKVVLRRVPNNQTEQNV 775
           + R++KNVG P  Y A+   P GV VSV+P  L F KT E K F++ LR +P   +   V
Sbjct: 700 VTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGY-V 759

BLAST of CmaCh16G010020 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 561.6 bits (1446), Expect = 9.8e-160
Identity = 327/733 (44.61%), Postives = 453/733 (61.80%), Query Frame = 0

Query: 84  SYIVYLGSHSHGLNPSAIDLQRATQTHYNLLGSMLGSNEAAKEAIFYSYNRHINAFAAVL 143
           +YIV++        PS+ DL      H N   S L S   + E + Y+Y   I+ F+  L
Sbjct: 31  TYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRL 90

Query: 144 DQKVAED-------IAIHENKKLKLHTTRSWNFLGVENDGGIPLDSLWNLSRFGESTIIG 203
            Q+ A+        I++    + +LHTTR+  FLG++         L+  +      ++G
Sbjct: 91  TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA----DLFPEAGSYSDVVVG 150

Query: 204 NIDSGVWPESKSFSDEGYGPIPTRWKGSCEGGTHFS---CNRKLIGVRYFNKGFASDVGP 263
            +D+GVWPESKS+SDEG+GPIP+ WKG CE GT+F+   CNRKLIG R+F +G+ S +GP
Sbjct: 151 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 210

Query: 264 LNSSYE--TARDVDGHGTHTLSTAGGNFVKGVSILGNSYGTAKGGSPKALVAAYKVCWST 323
           ++ S E  + RD DGHGTHT STA G+ V+G S+LG + GTA+G +P+A VA YKVCW  
Sbjct: 211 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL- 270

Query: 324 DRGDGCFMADILASFEAAISDGVDVLSVSLGGGIQEFSDDLIAIGSFHAVKNGITVVCSA 383
               GCF +DILA+ + AI+D V+VLS+SLGGG+ ++  D +AIG+F A++ GI V CSA
Sbjct: 271 ---GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSA 330

Query: 384 GNSGPSEDTVLNVAPWMITVGASTVDRLF---------TSYVGESLSSKLLPPKKFYPLI 443
           GN+GPS  ++ NVAPW+ TVGA T+DR F          ++ G SL      P K  P I
Sbjct: 331 GNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFI 390

Query: 444 RALDAKSNNTSNHEAILCRQGSLNPEKVRGKIVVCLRGENSRGEKGYVVAQAGGVGMILA 503
            A +A +    N    LC  G+L PEKV+GKIV+C RG N+R +KG VV  AGGVGMILA
Sbjct: 391 YAGNASNATNGN----LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILA 450

Query: 504 NDKESGDELSASPHFLPAS-----------HISYTDAYMTSV-----TELGIKPAPIMAS 563
           N   +G+EL A  H LPA+           H   TD   T+      T +G+KP+P++A+
Sbjct: 451 NTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAA 510

Query: 564 FSSRGPNIVEPSILKPDITAPGVNIIAAFSEATSISGLPYDKRQAQFITLSGTSMSCPHI 623
           FSSRGPN + P+ILKPD+ APGVNI+AA++ A   +GL  D R+ +F  +SGTSMSCPH+
Sbjct: 511 FSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHV 570

Query: 624 SGIVGLLKTLYPKWSPAAIRSAIMTTAETEANDLNPILTSEKEK-ANPLAYGAGHVQPNK 683
           SG+  LLK+++P+WSPAAIRSA+MTTA     D  P+L     K + P  +GAGHV P  
Sbjct: 571 SGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTT 630

Query: 684 ASNPGLVYDLTTQDYLNFLCARGYNKTLLKLFTNDTSFVC--SKSFKVTDLNYPSISMNN 743
           A+NPGL+YDLTT+DYL FLCA  Y    ++  +   ++ C  SKS+ V DLNYPS ++N 
Sbjct: 631 ATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSFAVNV 690

Query: 744 LKSEAVEIKRRVKNVGSPGMYVAQVEA-PPGVSVSVDPSTLKFTKTDEEKDFKVVLRRVP 776
               A +  R V +VG  G Y  +V +   GV +SV+P+ L F + +E+K + V      
Sbjct: 691 DGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDS 740

BLAST of CmaCh16G010020 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.6e-146
Identity = 303/684 (44.30%), Postives = 410/684 (59.94%), Query Frame = 0

Query: 109 THYNLLGSMLGSNEAAKEAIFYSYNRHINAFAAVLDQKVAEDIA--------IHENKKLK 168
           TH++   S L S    + ++ Y+Y    + F+A LD   A+ +         I E+    
Sbjct: 45  THHDWYTSQLNS----ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYT 104

Query: 169 LHTTRSWNFLGVENDGGIPLDSLWNLSRFGESTIIGNIDSGVWPESKSFSDEGYGPIPTR 228
           LHTTR+  FLG+ ++ G+      +L       IIG +D+GVWPES+SF D     IP++
Sbjct: 105 LHTTRTPEFLGLNSEFGV-----HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSK 164

Query: 229 WKGSCEGGTHFS---CNRKLIGVRYFNKGF-ASDVGPLNSSYETA--RDVDGHGTHTLST 288
           WKG CE G+ F    CN+KLIG R F+KGF  +  G  +S  E+   RDVDGHGTHT +T
Sbjct: 165 WKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTT 224

Query: 289 AGGNFVKGVSILGNSYGTAKGGSPKALVAAYKVCWSTDRGDGCFMADILASFEAAISDGV 348
           A G+ V+  S LG + GTA+G + +A VA YKVCWST    GCF +DILA+ + AI DGV
Sbjct: 225 AAGSAVRNASFLGYAAGTARGMATRARVATYKVCWST----GCFGSDILAAMDRAILDGV 284

Query: 349 DVLSVSLGGGIQEFSDDLIAIGSFHAVKNGITVVCSAGNSGPSEDTVLNVAPWMITVGAS 408
           DVLS+SLGGG   +  D IAIG+F A++ G+ V CSAGNSGP+  +V NVAPW++TVGA 
Sbjct: 285 DVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAG 344

Query: 409 TVDRLFTSYVGESLSSKLLPPKKFYPL---IRALDAKSNNTSNHEAILCRQGSLNPEKVR 468
           T+DR F ++       +L     +  +    + L+   N  ++  + LC  GSL+   VR
Sbjct: 345 TLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVR 404

Query: 469 GKIVVCLRGENSRGEKGYVVAQAGGVGMILANDKESGDELSASPHFLPASHIS------- 528
           GKIVVC RG N+R EKG VV  AGG+GMI+AN   SG+EL A  H LPA  +        
Sbjct: 405 GKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLL 464

Query: 529 ----YTDAYMTSV-----TELGIKPAPIMASFSSRGPNIVEPSILKPDITAPGVNIIAAF 588
                +D+  T++     T L +KP+P++A+FSSRGPN V P ILKPD+  PGVNI+A +
Sbjct: 465 REYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGW 524

Query: 589 SEATSISGLPYDKRQAQFITLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAET 648
           S+A   +GL  D R+ QF  +SGTSMSCPHISG+ GLLK  +P+WSP+AI+SA+MTTA  
Sbjct: 525 SDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYV 584

Query: 649 EANDLNPIL-TSEKEKANPLAYGAGHVQPNKASNPGLVYDLTTQDYLNFLCARGYNKTLL 708
             N   P+   ++   +NP A+G+GHV P KA +PGLVYD++T++Y+ FLC+  Y    +
Sbjct: 585 LDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHI 644

Query: 709 KLFTNDTSFVCSKSFK-VTDLNYPSISMNNLKSEAVEIKRRVKNVG-SPGMYVAQVEAPP 757
                  S  CSK F     LNYPS S+       V   R V NVG +  +Y   V   P
Sbjct: 645 VAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAP 704

BLAST of CmaCh16G010020 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 2.4e-142
Identity = 299/686 (43.59%), Postives = 406/686 (59.18%), Query Frame = 0

Query: 116 SMLGSNEAAKE---AIFYSYNRHINAFAAVLDQKVA-------EDIAIHENKKLKLHTTR 175
           ++LG  E  +E    + YSY   I  FAA L +  A       E +A+  +  L++ TT 
Sbjct: 56  AVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTY 115

Query: 176 SWNFLGVENDGGIPLDSLWNLSRFGESTIIGNIDSGVWPESKSFSDEGYGPIPTRWKGSC 235
           S+ FLG++  G      +W+ SRFG+ TIIG +D+GVWPES SF D G   IP +WKG C
Sbjct: 116 SYKFLGLDGFGN---SGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGIC 175

Query: 236 EGGTHF---SCNRKLIGVRYFNKGFASDVGPLNS-----SYETARDVDGHGTHTLSTAGG 295
           + G  F   SCNRKLIG R+F +G      P  S      Y +ARD  GHGTHT ST GG
Sbjct: 176 QEGESFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGG 235

Query: 296 NFVKGVSILGNSYGTAKGGSPKALVAAYKVCWSTDRGDGCFMADILASFEAAISDGVDVL 355
           + V   ++LGN  G A+G +P A +A YKVCW     +GC+ +DILA+ + AI D VDVL
Sbjct: 236 SSVSMANVLGNGAGVARGMAPGAHIAVYKVCWF----NGCYSSDILAAIDVAIQDKVDVL 295

Query: 356 SVSLGGGIQEFSDDLIAIGSFHAVKNGITVVCSAGNSGPSEDTVLNVAPWMITVGASTVD 415
           S+SLGG      DD IAIG+F A++ GI+V+C+AGN+GP E +V N APW+ T+GA T+D
Sbjct: 296 SLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLD 355

Query: 416 RLFTSYVGESLSSKLLPPKKFYP------LIRALDAKSNNTSNHEAILCRQGSLNPEKVR 475
           R F + V    + KLL  +  YP        R ++       +  +  C +GSL  E++R
Sbjct: 356 RRFPAVV-RLANGKLLYGESLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIR 415

Query: 476 GKIVVCLRGENSRGEKGYVVAQAGGVGMILANDKESGDELSASPHFLPASHISYTD---- 535
           GK+V+C RG N R EKG  V +AGGV MILAN + + +E S   H LPA+ I YT+    
Sbjct: 416 GKMVICDRGVNGRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLL 475

Query: 536 -AYMTSV-----------TELGIKPAPIMASFSSRGPNIVEPSILKPDITAPGVNIIAAF 595
            AY+ +            T +G   AP +A FS+RGP++  PSILKPD+ APGVNIIAA+
Sbjct: 476 KAYVNATVKPKARIIFGGTVIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAW 535

Query: 596 SEATSISGLPYDKRQAQFITLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAET 655
            +    +GLPYD R+  F  +SGTSMSCPH+SGI  L+++ YP WSPAAI+SA+MTTA+ 
Sbjct: 536 PQNLGPTGLPYDSRRVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADL 595

Query: 656 EANDLNPILTSEKEKANPLAYGAGHVQPNKASNPGLVYDLTTQDYLNFLCARGYNKTLLK 715
                  I    K  A   A GAGHV P KA NPGLVY++   DY+ +LC  G+ ++ + 
Sbjct: 596 YDRQGKAIKDGNK-PAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDIL 655

Query: 716 LFTNDTSFVCSKSFKVT---DLNYPSISMNNLKSEAVE-IKRRVKNVGSP-GMYVAQVEA 757
             T+  +  C+   +      LNYPSI++   + +  E I RRV NVGSP  +Y   V+A
Sbjct: 656 AITH-KNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKA 715

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSP52.9e-22554.67Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
F4JXC54.6e-22356.77Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
I1N4625.2e-19049.80Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
O653511.4e-15844.61Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF62.3e-14544.30Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Match NameE-valueIdentityDescription
AT2G04160.12.1e-22654.67Subtilisin-like serine endopeptidase family protein [more]
AT5G59810.13.3e-22456.77Subtilase family protein [more]
AT5G67360.19.8e-16044.61Subtilase family protein [more]
AT2G05920.11.6e-14644.30Subtilase family protein [more]
AT1G04110.12.4e-14243.59Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 577..593
score: 50.29
coord: 190..209
score: 24.96
coord: 263..276
score: 39.48
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 5..83
e-value: 8.5E-17
score: 61.2
coord: 693..774
e-value: 2.8E-18
score: 65.9
NoneNo IPR availableGENE3D3.50.30.30coord: 397..517
e-value: 2.9E-166
score: 555.8
NoneNo IPR availableGENE3D2.60.40.2310coord: 664..775
e-value: 2.3E-25
score: 90.8
NoneNo IPR availableGENE3D2.60.40.2310coord: 1..89
e-value: 2.2E-18
score: 68.1
NoneNo IPR availablePANTHERPTHR10795:SF662SUBTILISIN-LIKE PROTEASE SBT5.4coord: 83..773
NoneNo IPR availablePANTHERPTHR10795:SF662SUBTILISIN-LIKE PROTEASE SBT5.4coord: 3..81
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 166..647
score: 27.612562
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 401..509
e-value: 1.06553E-29
score: 112.122
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 432..515
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 84..154
e-value: 5.9E-9
score: 36.5
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 193..660
e-value: 2.9E-166
score: 555.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 161..651
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 191..639
e-value: 1.2E-42
score: 146.7
IPR003137PA domainPFAMPF02225PAcoord: 440..508
e-value: 1.2E-11
score: 44.6
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 83..773
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 3..81
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 578..588
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 159..613
e-value: 3.18361E-136
score: 403.132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G010020.1CmaCh16G010020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity