CmaCh04G022390 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G022390
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionGRAM domain-containing protein
LocationCma_Chr04: 15617903 .. 15621260 (+)
RNA-Seq ExpressionCmaCh04G022390
SyntenyCmaCh04G022390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATATCAATCTTCGTTTCTGGTAAAATTATGCGTCCGTTATGCCTTTTAGCATGTGATAGGTGTTAGTTTTACCAGTTGGGTTTTACATGGACCTTCCTTACCTTTTCTTTATGCTGGTATGGAAAGTAAGCTGGCATCAGAAAGCCAATATGCCAGCTCAAAAAGTTGAAAATCATAGCCTCACACCATTTCAAATTGAGGGAAAGTAGGAATCTATAGTGCTATGATTCAAAAGTTGTCTTGGGATCTATTTTCAATAGCTAGCTTTTTCTATACTTTCTTCTTCTTATTTATTTTATCAACTTTTTTCGGGGGTGCGAGTGGGAGTTGTTGAAATATCATCACTAGCCCAATAGAGTCAAGCCCAATAATTGTACTAGCCCAATAGAGTCAAGCCTAATTGTACGAGCCCAGTATATTTATTTTAGTAGAGATTTTAGGAGAAAGAGATGTAGATATTTTAGGAGAAAGATTCAATTTGTTAGGTCTAGATTCAAATCGGTTAGGTCTAGATTTAGATTGGTTAAATATAGGTTGTTTACTCTTAGTATAAATACCCCCACCCTACCTAGGTTATTCATCCAAGAAATCTAAAAACAATAAAAAGCAGAAGTATTCTACAGAGTGTCTTGTATTCTCTTCTCGATTGGTCTTAATAAAGAGAGCGAGTGTTTCCCACAGTGAGTTGTGTTCAACAGGAGTTCGTTAAGAAACCTGAACAATTTTCGTGTCGCCATAATTCATGGTAATAGATGTTTTAAATAAGAGGTACGTGAGGATAACTTAACTTGTTAAGTCATAATTATCCAACTAAAAGGTTGAGGCTGAAATCCTCACGTGTTGTTGAACTATTACTCTCTTGTTTCCTTGAGCTTTAGTGGATCCGCTCGGTAAGGTCCTTTTTCGGTAGTGCAGAGAAACAAAAAACAAAGTCATGAGAGTTTAGGCTCAAAGTGGACAATATCATACCATTGTGGAGGTTCGTAGTTCCTAACAGGTATCAGAGTCATGCCTTTAACTTAACCATGTTAATAGAATCCTCAAATGTCGAACAAAAAAGTTATGAGCCTCGAAAATGTAGTCAAAAGTGACTCAAATGTCGAACAAATAGTGTACTTTGTTTGAGGCTCTAGAGAAGGAGTCAAGTCTCGATTAAGAGGAGACTGTTCGAGGGCTCCATATGCCTAAGGGGGGGCTCTATGGTGTACTTGGTTTAAAGGATTGTTGGGAGGGAGTCTCACATTGGCTAATTAAGGGGAAGATCATGGAAATGAGGCCTTATGAGAGCTATTGCTAAAAGTGGACAATATCATAACATTATGGAGAGTCGTGGTTTCTAACATCCTCGTTATACTTACTTACACTCAGCACTTCTGCAATATACCGAGACCATCGGCTTTCATATTCTTATAACTCCAAGAAACTAGCCTTTCTTCAGAAGCGTTCTAGGGAGATAGAAGATATTAAAAAATGCATTCAGTTTTTCTTTTTTTCTGAAGTTACTAAACCTAAGATCCCCTTTTCTTTTTCCCAATTTGCTTAACTTTATTTTTTTTCTGATGCACATTGGATGACTCGGACAATAGAAAAGTAGGATTCACCAACAATTTTCGACCCCTTTAAATGTATTAGGAGAATCAAAGAATGATACACTAGGCATCAAGCAAACATCTCTTCCCCTGCTTATTGCTATTGAATAACATCCTAGGTGAGTGGCTGCAGAGTTCAAGCCGCTCAAAGAAAGAGCATTCTGTATTGCGGAGTTTTCTTTATCAATATTCGAATATGGAAGCTTCACTGCAGAGACATGTTATTGGATTTCCACTTGGCTCAGCAGCATTGCAGGTCGAGCAATCGGTTAAAAGACTCTTACCTGATCTTGCTGGCCAGTACAACATTCCCCCCTCAGATGATAAATATCCATCAAAACAATGTAAGAAATCCCCCCTACTCTTGGTTTGACAAATGAATGATGAGCAAGAAATGGACTTTTGGATTGAGAATAATCTTCATTTTGCAGGTAGTAAAAATTCTGTGCTTAGCAGGGTGAATAAGCTTGGAAAAAGAGCAGACAAGTTTGCACATGGAGTCCGAGAACATGGTGAGTTCATTGTTCTACTTTATTAGTTTCTTATTAAGAGTGTCAAATGCTTTGATACGGTGCTAATGTGAGATCCCACATTGGTTGGAGAGGGGAACGAAACATTCTTTATAAGAGTGTGGAAACCTCTCCCCAGTAGACGTGTTTTAAAAACCTTGAGGTGAAACCTGAAAGGGAAAGCCCAAAGAGGACAATATCGGCTAGCAGTGGACTTGAGCTGTTACAAATGATATCAGAGCCAGACATCGAGCGGTGTGCTATCGAGGACGCTCGGCCCTCAATGGAGGTGGATTGTGAGATCCCACGTCGGTTGGACAGGAGAACGAAACATTCATTATAAAAATGTGGAAACCTCTCCCTAGCAGACTCGTTTTAAAAACCTTTAGGGAAAGTTCAAAGAGGACAATATCTGCTAGCGGTGGGTGGATGAGAATTGAGATGATAACTAAGTTTTTCTTTTGTTTATTTATTACAACATCCAAGTGAGCTATTTGGAATGGAAATTTGATAGAAACTTTCCTGTTTATTGAACTGCAGTGAGATTGGGATCCAACATCTCAGAAATTGTTAAGGGAAAGTTGAGCTTAGGAGCTAGAATTCTTCAAGTAGGTGGTTTGAGAAAAATATACAAGCAATTGTTTATTGATGGAGATAGCGAGAAATTGTTGAAGGCTGCCCAATGCTATCTGTCAACCACAGCTGGACCCATTGCAGGTCTGCTCTTCATATCCACAGATAGGATTTCCTTCTGCAGTGACAGATCCATCAAAGTTTCTTCCTCCTCAGGAGAGCTAATTCGATTCCATTACAAGGTATGGTTGCTTTCTTGTTCTTCGTCATCGTCGTCAGTGTTGTCTTCGTCTTCTTTCTTTCTCTCTTTCTCTGCGCTAAATCTCAAAAGAAATGTTTGTGTTCTGCAGGTAGTGATTCCAGTAGGAAAAATAGAGAGAATCAATGAGAGTGAGAATGTGAAGAAGCCTTCACAGAAGTACATAGAAATAGTTACAGTTGATAATTTTGACTTCTGGTTCATGGGGTTCTTAAATTATCACAAAACTCTTCAATATCTTCAAAATTTGATCCCTTTAGAATGCAAGAACTGCTAAATGCTTGGAAGAGATTGCAGCAGAAATGTTCTTCGTCTCCTCCACCGTTTGAACTGGGAGGAAAAGTTCACTATACAATTTACTCAATTGTACAAATTCTGTTTATGAATGATTGGTTTAGTTGTGAAATAAGATTAGAAAGAGGCTC

mRNA sequence

ATGATATCAATCTTCGTTTCTGGTGAGTGGCTGCAGAGTTCAAGCCGCTCAAAGAAAGAGCATTCTGTATTGCGGAGTTTTCTTTATCAATATTCGAATATGGAAGCTTCACTGCAGAGACATGTTATTGGATTTCCACTTGGCTCAGCAGCATTGCAGGTCGAGCAATCGGTTAAAAGACTCTTACCTGATCTTGCTGGCCAGTACAACATTCCCCCCTCAGATGATAAATATCCATCAAAACAATGTAGTAAAAATTCTGTGCTTAGCAGGGTGAATAAGCTTGGAAAAAGAGCAGACAAGTTTGCACATGGAGTCCGAGAACATGTGAGATTGGGATCCAACATCTCAGAAATTGTTAAGGGAAAGTTGAGCTTAGGAGCTAGAATTCTTCAAGTAGGTGGTTTGAGAAAAATATACAAGCAATTGTTTATTGATGGAGATAGCGAGAAATTGTTGAAGGCTGCCCAATGCTATCTGTCAACCACAGCTGGACCCATTGCAGGTCTGCTCTTCATATCCACAGATAGGATTTCCTTCTGCAGTGACAGATCCATCAAAGTTTCTTCCTCCTCAGGAGAGCTAATTCGATTCCATTACAAGGTAGTGATTCCAGTAGGAAAAATAGAGAGAATCAATGAGAGTGAGAATGTGAAGAAGCCTTCACAGAAGTACATAGAAATAGTTACAGTTGATAATTTTGACTTCTGGTTCATGGGGTTCTTAAATTATCACAAAACTCTTCAATATCTTCAAAATTTGATCCCTTTAGAATGCAAGAACTGCTAAATGCTTGGAAGAGATTGCAGCAGAAATGTTCTTCGTCTCCTCCACCGTTTGAACTGGGAGGAAAAGTTCACTATACAATTTACTCAATTGTACAAATTCTGTTTATGAATGATTGGTTTAGTTGTGAAATAAGATTAGAAAGAGGCTC

Coding sequence (CDS)

ATGATATCAATCTTCGTTTCTGGTGAGTGGCTGCAGAGTTCAAGCCGCTCAAAGAAAGAGCATTCTGTATTGCGGAGTTTTCTTTATCAATATTCGAATATGGAAGCTTCACTGCAGAGACATGTTATTGGATTTCCACTTGGCTCAGCAGCATTGCAGGTCGAGCAATCGGTTAAAAGACTCTTACCTGATCTTGCTGGCCAGTACAACATTCCCCCCTCAGATGATAAATATCCATCAAAACAATGTAGTAAAAATTCTGTGCTTAGCAGGGTGAATAAGCTTGGAAAAAGAGCAGACAAGTTTGCACATGGAGTCCGAGAACATGTGAGATTGGGATCCAACATCTCAGAAATTGTTAAGGGAAAGTTGAGCTTAGGAGCTAGAATTCTTCAAGTAGGTGGTTTGAGAAAAATATACAAGCAATTGTTTATTGATGGAGATAGCGAGAAATTGTTGAAGGCTGCCCAATGCTATCTGTCAACCACAGCTGGACCCATTGCAGGTCTGCTCTTCATATCCACAGATAGGATTTCCTTCTGCAGTGACAGATCCATCAAAGTTTCTTCCTCCTCAGGAGAGCTAATTCGATTCCATTACAAGGTAGTGATTCCAGTAGGAAAAATAGAGAGAATCAATGAGAGTGAGAATGTGAAGAAGCCTTCACAGAAGTACATAGAAATAGTTACAGTTGATAATTTTGACTTCTGGTTCATGGGGTTCTTAAATTATCACAAAACTCTTCAATATCTTCAAAATTTGATCCCTTTAGAATGCAAGAACTGCTAA

Protein sequence

MISIFVSGEWLQSSSRSKKEHSVLRSFLYQYSNMEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC
Homology
BLAST of CmaCh04G022390 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.1e-54
Identity = 111/198 (56.06%), Postives = 144/198 (72.73%), Query Frame = 0

Query: 62  LPDLA--GQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREHVRLGSNISEI 121
           LPD A   ++ +P S  K  S+Q +  S+L R     K+ D F +GVR+  ++   ++E 
Sbjct: 24  LPDPASFNKFRVPASSKK--SEQSNVKSILKR-----KKTDGFTNGVRDQSKIRPKLTET 83

Query: 122 VKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAGLLFISTDRIS 181
           VK KLSLGARILQVGGL KI+K+LF   + EKL K  QCYLSTTAGPIAGLLFIS+ +++
Sbjct: 84  VKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMA 143

Query: 182 FCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIVTVDNFDFWFM 241
           FCS+RSIKV S  G++IR HYKV IP+ KI+R+N+S+N KKPSQKY+E+VTVD FDFWFM
Sbjct: 144 FCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFM 203

Query: 242 GFLNYHKTLQYLQNLIPL 258
           GFL+Y K    L+  + L
Sbjct: 204 GFLSYQKAFNCLEKALSL 214

BLAST of CmaCh04G022390 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.1e-54
Identity = 114/198 (57.58%), Postives = 138/198 (69.70%), Query Frame = 0

Query: 66  AGQYNIPPSDDKYPSKQCSKNSVLSRVNK---LGKRADKFAHGVREHVRLGSNISEIVKG 125
           AG    P S +K      SK S L+   K     K+ D F +GVR+  +LG  ++E VK 
Sbjct: 21  AGYLPDPASINKLQIPTSSKFSFLTGKGKSMLRKKKNDSFTNGVRDQDKLGPKLTETVKR 80

Query: 126 KLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAGLLFISTDRISFCS 185
           KLSLGARILQ+GGL KIYK+LF   D EKL KA QCYLSTTAGPIAGLLFIS+ +I+FCS
Sbjct: 81  KLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCS 140

Query: 186 DRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIVTVDNFDFWFMGFL 245
           +RSIKV+S  GEL R HYKV IP+ KI  +N+S+N  KPSQKY+E+VTVD FDFWFMGFL
Sbjct: 141 ERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNTTKPSQKYLEVVTVDGFDFWFMGFL 200

Query: 246 NYHKTLQYLQNLIPLECK 261
           +Y K    L+  + L  K
Sbjct: 201 SYQKAFNCLEQALSLSFK 218

BLAST of CmaCh04G022390 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 206.8 bits (525), Expect = 2.9e-52
Identity = 114/221 (51.58%), Postives = 144/221 (65.16%), Query Frame = 0

Query: 38  LQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNK--- 97
           + + VI FP        +      LPD       P S + +     SK S L+   K   
Sbjct: 6   VHQQVIAFP------AAKTDTMSYLPD-------PASINNHQIPTSSKVSYLTGKGKSML 65

Query: 98  LGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLK 157
             K+ D F +G R+  +LG  ++E VK KLSLGA+ILQ+GGL KIYK+LF   D EKL K
Sbjct: 66  RKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFK 125

Query: 158 AAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINE 217
           A QCYLSTTAGPIAGLLFIS+ +I+FCS+RSIKV+S  G L R HYKV IP+ KI  +N+
Sbjct: 126 AYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQ 185

Query: 218 SENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLI 256
           S+N KKPSQKY+EIVT+DNFDFWFMGF++Y K    L+  +
Sbjct: 186 SQNTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKAL 213

BLAST of CmaCh04G022390 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 3.2e-51
Identity = 111/218 (50.92%), Postives = 144/218 (66.06%), Query Frame = 0

Query: 38  LQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGK 97
           + + VI FP       V  +    LPD       P S +K      SK S+L +     K
Sbjct: 6   VHQQVIAFP------AVNTAPVGYLPD-------PASINKLQIPTSSKVSMLQK-----K 65

Query: 98  RADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQ 157
           + D F +G R+  +LG  ++E VK KLSLGA+ILQ+GGL KIYK+LF   D EKL KA Q
Sbjct: 66  KTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQ 125

Query: 158 CYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESEN 217
           CYLSTT G IAGLLFIS+ +I+FCS+RSIKV+S  G+L R HYKV IP+ KI  +N+S+N
Sbjct: 126 CYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQN 185

Query: 218 VKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLI 256
            KKPSQ+Y+E+VTVDN+DFWFMGF++Y K    L+  +
Sbjct: 186 TKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKAL 205

BLAST of CmaCh04G022390 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.4e-30
Identity = 73/203 (35.96%), Postives = 124/203 (61.08%), Query Frame = 0

Query: 53  QVEQSVKRLLPDLAGQYNIPPSDDKYP---SKQCSKNSVLSRVNKLGK-------RADKF 112
           +++ + K       G+Y+ P      P   S + SK+ VL  +N+ GK       +A+  
Sbjct: 11  KLDSTAKHKTNSHGGRYDNPYVHITSPTSASDKRSKDKVLEVLNRCGKKVEDATRKAEAL 70

Query: 113 AHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLST 172
             G+++H++   +IS+    +LS G +++  GG  +++++ F     EKLL +  CY+ST
Sbjct: 71  VGGLKDHLKFSPSISDAAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYIST 130

Query: 173 TAGPIAGLLFISTDRISFCSDRSIKV-SSSSGELIRFHYKVVIPVGKIERINESENVKKP 232
           T+GP+ G+++IS  RI+FCSD +I++ SS+ G  +  +YKVV+   KI  I+ S NV KP
Sbjct: 131 TSGPVTGVIYISNRRIAFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKP 190

Query: 233 SQKYIEIVTVDNFDFWFMGFLNY 245
           S++Y+ +VT D F+FWFMGF++Y
Sbjct: 191 SERYVHMVTRDGFEFWFMGFVSY 213

BLAST of CmaCh04G022390 vs. ExPASy TrEMBL
Match: A0A6J1JM22 (GEM-like protein 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487106 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 7.8e-125
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 34  MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN 93
           MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN
Sbjct: 1   MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN 60

Query: 94  KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL 153
           KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL
Sbjct: 61  KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL 120

Query: 154 KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 213
           KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN
Sbjct: 121 KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 180

Query: 214 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC
Sbjct: 181 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 229

BLAST of CmaCh04G022390 vs. ExPASy TrEMBL
Match: A0A6J1GPT0 (putative GEM-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111456007 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.2e-120
Identity = 222/229 (96.94%), Postives = 226/229 (98.69%), Query Frame = 0

Query: 34  MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN 93
           MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSD KYPSKQCSKNSVLSRVN
Sbjct: 1   MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDGKYPSKQCSKNSVLSRVN 60

Query: 94  KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL 153
           KLGK+ADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLF DGDSEKLL
Sbjct: 61  KLGKKADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFSDGDSEKLL 120

Query: 154 KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 213
           KAAQCYLSTTAGPIAGLLFISTD+I+FCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN
Sbjct: 121 KAAQCYLSTTAGPIAGLLFISTDKIAFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 180

Query: 214 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           ESENVKKPSQKYIEIVTVDNFDFWFMGFLNY+KTLQYLQNLI LECKNC
Sbjct: 181 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYYKTLQYLQNLISLECKNC 229

BLAST of CmaCh04G022390 vs. ExPASy TrEMBL
Match: A0A6J1JSB8 (GEM-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487106 PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 4.3e-115
Identity = 212/213 (99.53%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 50  AALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREH 109
           +ALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREH
Sbjct: 7   SALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREH 66

Query: 110 VRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAG 169
           VRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAG
Sbjct: 67  VRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAG 126

Query: 170 LLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIV 229
           LLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIV
Sbjct: 127 LLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIV 186

Query: 230 TVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           TVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC
Sbjct: 187 TVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 219

BLAST of CmaCh04G022390 vs. ExPASy TrEMBL
Match: A0A1S3CN28 (putative GEM-like protein 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502808 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 6.2e-106
Identity = 200/240 (83.33%), Postives = 215/240 (89.58%), Query Frame = 0

Query: 24  LRSFLYQYSNMEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPS-KQ 83
           +R+FL  +SN     +RHVIGFP+GS ALQVEQSV+RLLPDLAGQYNIP SD KYP+ KQ
Sbjct: 16  MRTFLIFFSN-----KRHVIGFPIGSTALQVEQSVQRLLPDLAGQYNIPSSDTKYPTLKQ 75

Query: 84  CSKNSVLSRVNKLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQ 143
           C K+S+LSRVNKLGKRAD FAHGVREHVRLGS ISE VKGKLSLGARILQVGGLRKI+KQ
Sbjct: 76  CGKDSMLSRVNKLGKRADSFAHGVREHVRLGSKISETVKGKLSLGARILQVGGLRKIFKQ 135

Query: 144 LFIDGDSEKLLKAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKV 203
           LF  GD EKLLKA  CYLSTTAGPIAGLLFISTD+I+FCSDRSIKVSS SGELIRFHYKV
Sbjct: 136 LFNVGDGEKLLKAVHCYLSTTAGPIAGLLFISTDKIAFCSDRSIKVSSPSGELIRFHYKV 195

Query: 204 VIPVGKIERINESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           VIPVGKIERIN+SENVKKPSQKY+EIVTVDNFDFWFMGFLNYHKTLQYLQ +I  ECK C
Sbjct: 196 VIPVGKIERINQSENVKKPSQKYMEIVTVDNFDFWFMGFLNYHKTLQYLQEVISQECKKC 250

BLAST of CmaCh04G022390 vs. ExPASy TrEMBL
Match: A0A5D3CUH3 (Putative GEM-like protein 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00540 PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.1e-105
Identity = 198/230 (86.09%), Postives = 209/230 (90.87%), Query Frame = 0

Query: 34  MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPS-KQCSKNSVLSRV 93
           ME  LQRHVIGFP+GS ALQVEQSV+RLL DLAGQYNIP SD KYP+ KQC K+S+LSRV
Sbjct: 1   METLLQRHVIGFPIGSTALQVEQSVQRLLSDLAGQYNIPSSDTKYPTLKQCGKDSMLSRV 60

Query: 94  NKLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKL 153
           NKLGKRAD FAHGVREHVRLGS ISE VKGKLSLGARILQVGGLRKI+KQLF  GD EKL
Sbjct: 61  NKLGKRADSFAHGVREHVRLGSKISETVKGKLSLGARILQVGGLRKIFKQLFNVGDGEKL 120

Query: 154 LKAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERI 213
           LKA  CYLSTTAGPIAGLLFISTD+I+FCSDRSIKVSS SGELIRFHYKVVIPVGKIERI
Sbjct: 121 LKAVHCYLSTTAGPIAGLLFISTDKIAFCSDRSIKVSSPSGELIRFHYKVVIPVGKIERI 180

Query: 214 NESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           N+SENVKKPSQKY+EIVTVDNFDFWFMGFLNYHKTLQYLQ +I  ECK C
Sbjct: 181 NQSENVKKPSQKYMEIVTVDNFDFWFMGFLNYHKTLQYLQEVISQECKKC 230

BLAST of CmaCh04G022390 vs. NCBI nr
Match: XP_022990116.1 (GEM-like protein 4 isoform X1 [Cucurbita maxima] >XP_022990117.1 GEM-like protein 4 isoform X1 [Cucurbita maxima])

HSP 1 Score: 456.4 bits (1173), Expect = 1.6e-124
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 34  MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN 93
           MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN
Sbjct: 1   MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN 60

Query: 94  KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL 153
           KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL
Sbjct: 61  KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL 120

Query: 154 KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 213
           KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN
Sbjct: 121 KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 180

Query: 214 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC
Sbjct: 181 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 229

BLAST of CmaCh04G022390 vs. NCBI nr
Match: XP_022953469.1 (putative GEM-like protein 8 [Cucurbita moschata] >XP_022953476.1 putative GEM-like protein 8 [Cucurbita moschata])

HSP 1 Score: 442.6 bits (1137), Expect = 2.4e-120
Identity = 222/229 (96.94%), Postives = 226/229 (98.69%), Query Frame = 0

Query: 34  MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN 93
           MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSD KYPSKQCSKNSVLSRVN
Sbjct: 1   MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDGKYPSKQCSKNSVLSRVN 60

Query: 94  KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL 153
           KLGK+ADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLF DGDSEKLL
Sbjct: 61  KLGKKADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFSDGDSEKLL 120

Query: 154 KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 213
           KAAQCYLSTTAGPIAGLLFISTD+I+FCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN
Sbjct: 121 KAAQCYLSTTAGPIAGLLFISTDKIAFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 180

Query: 214 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           ESENVKKPSQKYIEIVTVDNFDFWFMGFLNY+KTLQYLQNLI LECKNC
Sbjct: 181 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYYKTLQYLQNLISLECKNC 229

BLAST of CmaCh04G022390 vs. NCBI nr
Match: XP_023526338.1 (GEM-like protein 7 [Cucurbita pepo subsp. pepo] >XP_023526343.1 GEM-like protein 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 430.6 bits (1106), Expect = 9.4e-117
Identity = 216/229 (94.32%), Postives = 222/229 (96.94%), Query Frame = 0

Query: 34  MEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVN 93
           MEASL RHVIGFPLGSAALQVEQSVKR+LPDLAGQYNI PSD KYPSKQCSK SVLSRVN
Sbjct: 1   MEASLHRHVIGFPLGSAALQVEQSVKRILPDLAGQYNISPSDGKYPSKQCSKISVLSRVN 60

Query: 94  KLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLL 153
           KLGK+ADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLF DGDSEKLL
Sbjct: 61  KLGKKADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFSDGDSEKLL 120

Query: 154 KAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERIN 213
           KAAQCYLSTTAGPIAGLLFISTD+I+FCSDRSIKVSSS+GELIRFHYKVVIPVGKIERIN
Sbjct: 121 KAAQCYLSTTAGPIAGLLFISTDKIAFCSDRSIKVSSSAGELIRFHYKVVIPVGKIERIN 180

Query: 214 ESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           ESENVKKPSQKYIEIVTVDNF+FWFMGFLNYHKTLQYLQNLI  ECKNC
Sbjct: 181 ESENVKKPSQKYIEIVTVDNFEFWFMGFLNYHKTLQYLQNLISQECKNC 229

BLAST of CmaCh04G022390 vs. NCBI nr
Match: XP_022990118.1 (GEM-like protein 4 isoform X2 [Cucurbita maxima])

HSP 1 Score: 424.1 bits (1089), Expect = 8.8e-115
Identity = 212/213 (99.53%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 50  AALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREH 109
           +ALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREH
Sbjct: 7   SALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREH 66

Query: 110 VRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAG 169
           VRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAG
Sbjct: 67  VRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAG 126

Query: 170 LLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIV 229
           LLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIV
Sbjct: 127 LLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIV 186

Query: 230 TVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           TVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC
Sbjct: 187 TVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 219

BLAST of CmaCh04G022390 vs. NCBI nr
Match: XP_008465126.2 (PREDICTED: putative GEM-like protein 8 isoform X1 [Cucumis melo])

HSP 1 Score: 393.7 bits (1010), Expect = 1.3e-105
Identity = 200/240 (83.33%), Postives = 215/240 (89.58%), Query Frame = 0

Query: 24  LRSFLYQYSNMEASLQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPS-KQ 83
           +R+FL  +SN     +RHVIGFP+GS ALQVEQSV+RLLPDLAGQYNIP SD KYP+ KQ
Sbjct: 16  MRTFLIFFSN-----KRHVIGFPIGSTALQVEQSVQRLLPDLAGQYNIPSSDTKYPTLKQ 75

Query: 84  CSKNSVLSRVNKLGKRADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQ 143
           C K+S+LSRVNKLGKRAD FAHGVREHVRLGS ISE VKGKLSLGARILQVGGLRKI+KQ
Sbjct: 76  CGKDSMLSRVNKLGKRADSFAHGVREHVRLGSKISETVKGKLSLGARILQVGGLRKIFKQ 135

Query: 144 LFIDGDSEKLLKAAQCYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKV 203
           LF  GD EKLLKA  CYLSTTAGPIAGLLFISTD+I+FCSDRSIKVSS SGELIRFHYKV
Sbjct: 136 LFNVGDGEKLLKAVHCYLSTTAGPIAGLLFISTDKIAFCSDRSIKVSSPSGELIRFHYKV 195

Query: 204 VIPVGKIERINESENVKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLIPLECKNC 263
           VIPVGKIERIN+SENVKKPSQKY+EIVTVDNFDFWFMGFLNYHKTLQYLQ +I  ECK C
Sbjct: 196 VIPVGKIERINQSENVKKPSQKYMEIVTVDNFDFWFMGFLNYHKTLQYLQEVISQECKKC 250

BLAST of CmaCh04G022390 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 214.9 bits (546), Expect = 7.6e-56
Identity = 111/198 (56.06%), Postives = 144/198 (72.73%), Query Frame = 0

Query: 62  LPDLA--GQYNIPPSDDKYPSKQCSKNSVLSRVNKLGKRADKFAHGVREHVRLGSNISEI 121
           LPD A   ++ +P S  K  S+Q +  S+L R     K+ D F +GVR+  ++   ++E 
Sbjct: 24  LPDPASFNKFRVPASSKK--SEQSNVKSILKR-----KKTDGFTNGVRDQSKIRPKLTET 83

Query: 122 VKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAGLLFISTDRIS 181
           VK KLSLGARILQVGGL KI+K+LF   + EKL K  QCYLSTTAGPIAGLLFIS+ +++
Sbjct: 84  VKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMA 143

Query: 182 FCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIVTVDNFDFWFM 241
           FCS+RSIKV S  G++IR HYKV IP+ KI+R+N+S+N KKPSQKY+E+VTVD FDFWFM
Sbjct: 144 FCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFM 203

Query: 242 GFLNYHKTLQYLQNLIPL 258
           GFL+Y K    L+  + L
Sbjct: 204 GFLSYQKAFNCLEKALSL 214

BLAST of CmaCh04G022390 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 213.4 bits (542), Expect = 2.2e-55
Identity = 114/198 (57.58%), Postives = 138/198 (69.70%), Query Frame = 0

Query: 66  AGQYNIPPSDDKYPSKQCSKNSVLSRVNK---LGKRADKFAHGVREHVRLGSNISEIVKG 125
           AG    P S +K      SK S L+   K     K+ D F +GVR+  +LG  ++E VK 
Sbjct: 21  AGYLPDPASINKLQIPTSSKFSFLTGKGKSMLRKKKNDSFTNGVRDQDKLGPKLTETVKR 80

Query: 126 KLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAGLLFISTDRISFCS 185
           KLSLGARILQ+GGL KIYK+LF   D EKL KA QCYLSTTAGPIAGLLFIS+ +I+FCS
Sbjct: 81  KLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCS 140

Query: 186 DRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIVTVDNFDFWFMGFL 245
           +RSIKV+S  GEL R HYKV IP+ KI  +N+S+N  KPSQKY+E+VTVD FDFWFMGFL
Sbjct: 141 ERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNTTKPSQKYLEVVTVDGFDFWFMGFL 200

Query: 246 NYHKTLQYLQNLIPLECK 261
           +Y K    L+  + L  K
Sbjct: 201 SYQKAFNCLEQALSLSFK 218

BLAST of CmaCh04G022390 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 206.8 bits (525), Expect = 2.1e-53
Identity = 121/254 (47.64%), Postives = 156/254 (61.42%), Query Frame = 0

Query: 15  SRSKKEHSVLRSFLYQYSNMEASLQ----------RHVIGFPLGSAALQVEQSVKRLLPD 74
           S S   H++  SF +  S ++ + Q          + VI FP        +      LPD
Sbjct: 35  SSSTITHNLSLSFSFTKSRIKITTQSSKMTLSRVHQQVIAFP------AAKTDTMSYLPD 94

Query: 75  LAGQYNIPPSDDKYPSKQCSKNSVLSRVNK---LGKRADKFAHGVREHVRLGSNISEIVK 134
                  P S + +     SK S L+   K     K+ D F +G R+  +LG  ++E VK
Sbjct: 95  -------PASINNHQIPTSSKVSYLTGKGKSMLRKKKTDSFTNGARDQDKLGPKLTETVK 154

Query: 135 GKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLSTTAGPIAGLLFISTDRISFC 194
            KLSLGA+ILQ+GGL KIYK+LF   D EKL KA QCYLSTTAGPIAGLLFIS+ +I+FC
Sbjct: 155 RKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFC 214

Query: 195 SDRSIKVSSSSGELIRFHYKVVIPVGKIERINESENVKKPSQKYIEIVTVDNFDFWFMGF 254
           S+RSIKV+S  G L R HYKV IP+ KI  +N+S+N KKPSQKY+EIVT+DNFDFWFMGF
Sbjct: 215 SERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKKPSQKYLEIVTIDNFDFWFMGF 274

Query: 255 LNYHKTLQYLQNLI 256
           ++Y K    L+  +
Sbjct: 275 VSYQKAFNCLEKAL 275

BLAST of CmaCh04G022390 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 203.4 bits (516), Expect = 2.3e-52
Identity = 111/218 (50.92%), Postives = 144/218 (66.06%), Query Frame = 0

Query: 38  LQRHVIGFPLGSAALQVEQSVKRLLPDLAGQYNIPPSDDKYPSKQCSKNSVLSRVNKLGK 97
           + + VI FP       V  +    LPD       P S +K      SK S+L +     K
Sbjct: 6   VHQQVIAFP------AVNTAPVGYLPD-------PASINKLQIPTSSKVSMLQK-----K 65

Query: 98  RADKFAHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQ 157
           + D F +G R+  +LG  ++E VK KLSLGA+ILQ+GGL KIYK+LF   D EKL KA Q
Sbjct: 66  KTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQ 125

Query: 158 CYLSTTAGPIAGLLFISTDRISFCSDRSIKVSSSSGELIRFHYKVVIPVGKIERINESEN 217
           CYLSTT G IAGLLFIS+ +I+FCS+RSIKV+S  G+L R HYKV IP+ KI  +N+S+N
Sbjct: 126 CYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQN 185

Query: 218 VKKPSQKYIEIVTVDNFDFWFMGFLNYHKTLQYLQNLI 256
            KKPSQ+Y+E+VTVDN+DFWFMGF++Y K    L+  +
Sbjct: 186 TKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKAL 205

BLAST of CmaCh04G022390 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 134.0 bits (336), Expect = 1.7e-31
Identity = 73/203 (35.96%), Postives = 124/203 (61.08%), Query Frame = 0

Query: 53  QVEQSVKRLLPDLAGQYNIPPSDDKYP---SKQCSKNSVLSRVNKLGK-------RADKF 112
           +++ + K       G+Y+ P      P   S + SK+ VL  +N+ GK       +A+  
Sbjct: 11  KLDSTAKHKTNSHGGRYDNPYVHITSPTSASDKRSKDKVLEVLNRCGKKVEDATRKAEAL 70

Query: 113 AHGVREHVRLGSNISEIVKGKLSLGARILQVGGLRKIYKQLFIDGDSEKLLKAAQCYLST 172
             G+++H++   +IS+    +LS G +++  GG  +++++ F     EKLL +  CY+ST
Sbjct: 71  VGGLKDHLKFSPSISDAAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYIST 130

Query: 173 TAGPIAGLLFISTDRISFCSDRSIKV-SSSSGELIRFHYKVVIPVGKIERINESENVKKP 232
           T+GP+ G+++IS  RI+FCSD +I++ SS+ G  +  +YKVV+   KI  I+ S NV KP
Sbjct: 131 TSGPVTGVIYISNRRIAFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKP 190

Query: 233 SQKYIEIVTVDNFDFWFMGFLNY 245
           S++Y+ +VT D F+FWFMGF++Y
Sbjct: 191 SERYVHMVTRDGFEFWFMGFVSY 213

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FTA01.1e-5456.06GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1[more]
Q9FMW43.1e-5457.58Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV... [more]
Q9FMW62.9e-5251.58GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2[more]
Q9FMW53.2e-5150.92GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1[more]
Q9M1222.4e-3035.96GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JM227.8e-125100.00GEM-like protein 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487106 PE=3 S... [more]
A0A6J1GPT01.2e-12096.94putative GEM-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111456007 PE=3 S... [more]
A0A6J1JSB84.3e-11599.53GEM-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487106 PE=3 S... [more]
A0A1S3CN286.2e-10683.33putative GEM-like protein 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502808 P... [more]
A0A5D3CUH31.1e-10586.09Putative GEM-like protein 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
XP_022990116.11.6e-124100.00GEM-like protein 4 isoform X1 [Cucurbita maxima] >XP_022990117.1 GEM-like protei... [more]
XP_022953469.12.4e-12096.94putative GEM-like protein 8 [Cucurbita moschata] >XP_022953476.1 putative GEM-li... [more]
XP_023526338.19.4e-11794.32GEM-like protein 7 [Cucurbita pepo subsp. pepo] >XP_023526343.1 GEM-like protein... [more]
XP_022990118.18.8e-11599.53GEM-like protein 4 isoform X2 [Cucurbita maxima][more]
XP_008465126.21.3e-10583.33PREDICTED: putative GEM-like protein 8 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G08350.17.6e-5656.06GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23370.12.2e-5557.58GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23350.12.1e-5347.64GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23360.12.3e-5250.92GRAM domain-containing protein / ABA-responsive protein-related [more]
AT4G01600.11.7e-3135.96GRAM domain family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 138..216
e-value: 6.5E-16
score: 68.9
IPR004182GRAM domainPFAMPF02893GRAMcoord: 148..255
e-value: 2.3E-16
score: 59.7
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 136..256
e-value: 8.3E-20
score: 72.6
IPR037848GEM-like proteinPANTHERPTHR31969GEM-LIKE PROTEIN 2coord: 34..255
NoneNo IPR availablePANTHERPTHR31969:SF38GEM-LIKE PROTEIN 4coord: 34..255

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G022390.1CmaCh04G022390.1mRNA