CmoCh16G008070 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G008070
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmechanosensitive ion channel protein 2, chloroplastic-like isoform X1
LocationCmo_Chr16: 4154484 .. 4162845 (+)
RNA-Seq ExpressionCmoCh16G008070
SyntenyCmoCh16G008070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAAACAGAGACGGCGCCTCGATTGGAAGCTCTACAGCTTTGGGCATCAACAAAATTTGATTCCATTTCTGTAGGTAACATCTTAAGCCTTCAATCCTAGATTTCCTGTTCTGCCAATCTCAACTTTGGCGTTCTTCTCTTGTTCTTTGTTTTTGTTTTTGTTTTCTTATTCCACCACCGTCCTCGTTATCATCGCGAGTTTTTAGATGTTCGTCGATTACTCAATTTGAGATCTATTGCATGTTATAATCTGTATGTTTGGATTCTGCTCAAACGCTTGAGATTATGTTTCCTTTTGTTCTACCGTTTTGACTTTTTTTTTTTTCTTCAGAAGATACTGTTCGTAGGATTCTAATCCGCGATTGAGTCTTGATCTGAATGTTAGGGTTTGGAGAAAAAAATGTTGCGGTTTTTGGTTGAGTTATTGTTTTCGTTTATTAATTCACTTTGACCTTTTTTATTATCATGATGCTTAAATCGAGGATACATTAAGTTTCGTACTTTGGTTGGCGCTTTGTACGGGCTAATATTTTTTTCTCTCTTTTTGGTGAGAAACCGAGTTTTCATTGAGGAATGCTATAAAAAAGCCGGAAAATGAATTTCAATCCAAAGGAATTAACTGAAGCTGAGAATTACAAAATGACGATTTTTCCCCAAAAGACGCATTAAACTTCTTTGTGTCTCCATCCTTCTGAAAATTATACTATTCCTCTGAAGCCACTCTCCACGAGAACGATTCTGCCACAAGGCTCTTCCTTGATCATGAAATGGAAAGTTTATCATAATGTCCTCCAACATAGAGCAACAGGGTACAAACAAACTTAAAAATCTATTACGTGCCACATCAATCCCAAACACCTTAAAAATCTGACTCCTCAGGGTATAAGCAAACTGCGATCTCAAAAAAGATGATTGAGATCCTCCTCTTGTCGACGACAAATGAAACGTCACTGCAGATGCAAAACTAAGGAAGAAAATTGCTAGTTGCAAACCTGGGTGTTTACTCTACCATGTAAGACTTGCTAAGCAAATAACCTCACTTTTTTTAGGGACTTTGACCTTCCAAAGAGAGAGAGAGAAAAAAAAAAAAAAAAAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAACTGAGGCCTCTGACGGGGAGAAAGGAAACAAAGAATGAGGAAAAAGATTGACATGGGAATCCTTCTGATTGATTTGGAACCCAAACCTGAACATCCCTCCCACTAAAAAAAACCCTATGATTGAAGATGCTGGAAAGAAGTCCCACTACCTCCAAAGACTTACGATCACTCAAAGGACGACAAAACTGAGGTCACAAATATGACCTCTTTTACCCTGATGAACTGGCAACTTGGCAGGACCACGCAATGTGAAATGGTTTTCTATTTTCTTGTTTTGCTGAAAATATTTAAAACCAGCATCCTCCACTTGTTCGATGTATGTGACAAAATAATTTGATAGCGTACAACACTGTGTGTGCATGCCTGCACGTATACTGTTAGAAGTCGATGTTCATTGCCCAATTAACGAGCATATGTGGATTATGAACTCCTGTTTCACTTTATTGTGTTTCTTCTTTTAATCAATTTGAATATAATTTGCTGATGCAAGTTAGAGAACCAAGGTGTGAGACATAATTTAGATTCTTGACTATCAATTAATAGAAGTTGGTTCTACACCATTTATATTGCATTCATGATTCATGTATCAATAAAGGATGATTTGGTGCAGTTTTCAGCCGTCACTGTGCTGCGTAGAAGTCTCATTGTTCAATTTTCATCCTCCCAGCATTTAGTTAGTCTTAGCAATGGTTCTTGTTGGTTCTTTGCAACTGTCCTATCATCTGGGACCGTGGAAAAACCATTTGCTTGAAGAAAATCTTAAGGTACAGAGACCTTCTCCCCTGCCTCAAGACTATAAATATTTATTAGTCTCAGGTAAATATATGTGTTTCATTGTCTCTTTTTGTTCTTATCCAAAATTTAATTGTTCTTTTCAGTTTACCTCTCATAGCAATAACATACGGTTACAGAATGTAGCCAGTCCATCGTCTCTTTTAGTAAGTTCTTTAAACACTTCAGTTTAATTTTTAAAATCAGAACACAAATACCTTTTGTCCTTGGAGGTCATCAAGAATGCTTCCTTTATATCAAAATGGGATTGGATGATATCAATGTGTATTTGGCTGTGTATACTTGATGCATTGATTGCATATAGAAATTCTTGCCTTCAAGAGTAAATATTTGAGAATATTTCTTATTGCAGTTTCAGCAGAAGAACACTTGGAGTAGTCATTTATTTAGCTTGAAATACCCACCTAGTTACGTTGTGCCTTCTAGATACAATGCATTCAGGTGCCATTCGTCATTGATGACAAACCAACCACTGGATCCCTCTGGGATGAAGGCTGCTATAGTGGCGTTGACAAGGTGAGTTTTGTCTGGTTCATGTATCAATATTCAATGCCTCAAAAAATTTCCCAGCTTTGACTTATGTAAGATTGTTCTTAGTGTCATAGGGAGAATAAAGATATTATCTTTATCCCTGTTTTCACGCCCATCCACTATTAACATCAAAGCTACCACATTCATTATAAAAAAATCAACCCATCTGGATGCAGTTTACGTCAGTTTATTATTTTGTTCCCAAATGTTGGTTTGCATTTCATTTGGCCCTGTTGTGAAAAATTTATGTTGGTTAATGGCTATAATATGAAGGAAATTTTCATTAGAACTTTAGATGGATACATAATTGCTTACACGGGTGTGTTGGATAAATGTATTACAATTTATCTCTAATCATGAGACAAGTTGAACAAAGGACAATATTAGAGCTTTGAGAAAATATTAAAATTGGGGATTTTGGATCAGCGGCATTTTCTAAAAAATACTTGTTTGAGTCTAGCATCTGTGAAAATATTCTTTACATCATCCTTCCTTTGAAGAATGCTTACACTATCTCCAGAAAACCGCTATTTCTTCGGGCTTCAAATGCCTGGCAAATTTTATTCTCTCATTCTTGGCTTTTTGGTTTTTCACACATCTCTACCATCACACTAAAAAGAGATTATTGGAGGAGGCCACCATGGCTAGCGATTTTGCATTATTGGATTTATGAGAGGGAAATTGCAAAAATATTTCAAATTTGTATCTAGTGGTTATTAGGGGTATGCAAATTCTTGAATCTATATTTGACGGTCTTTAATATGCAACCCCATTATGTGGCCTCAATTTGAGGTTTATCTTGGACCTTGTGCTATGAACTATATTTTCCTTCTAGAGGTAAATTTTAACGCATAGTTAACCAACCACACTAATATTGTTGCAAATTAAGCAACTAGTAAGGCATCATATTTTTGTTGAAACATCGCAAGCACAGTTGATGGCCAGGACTAATATGGTGATTTGATTAAAAGCTAGGAACTTGTGTGCTTATATCATGAAAACTCAAATAGAAAGTAGCCATTAATTTAGGAAGAAAATTTGTAGTTTAACCTCATATTTTTTTACTAATGAAATTAAGATGATTGAGCACTTTAGTTTTTCATGGCGTTACCATAATTTTAATGGTACATTCGTTATCACTGTTCTACTGGTGTTGATTGACAATTTGCCATTTTTTTTTTTACTAGTATAAGGTATTTATTTCAATTTAGTTAGCGATATTTATGGGCATATAATCTTAAAGCTAAATATATATTTTGTTCCTGAATGTATGTGCTGTTTTCTATTTGTTTCTTCAATCTTCTAAAAAAAGTTTGAATGCTATCCTTTATATATTAAGTTTTATCACTCAGTTCCTTCTTGTAGTACACGTTAAAAGAATGGCTCCAGTAAAATGTTGTGGGTTGTGGTATGTGAAGTGGATAATCTTTTTTCTTTTTATATGTGGGGGAGTTCAATCAATAAAATTAAAGCATAGGGCAGTTTCATTTCAACTTGGTTAATAAAATATATTTTAATGTTCAATTATGACTTCTTTAGTAGATTTCAAGGTTTAGTGAGTCAGTAATGACTTGATAAAAAACCTTATCTTATGGTGGATGAAACTAAAATTTTGGAAAGGCTAGGGACAAAATTGAAGTGTGGCACAAACATATAAAGCACATAACAGTATATATTTAGTTTAATCTTAATTGAGATGTTCACTCTGGCATTTGCCTTGTAGACTCTAGAAATTAATTTAAATACATTTTTCATCTTTGAGTAATTAATATATTTCCATTGTGGCAAAATATCCGTCTCTTATATCATTTATTTTAGTTTCTACATGAGCTTGAAGATTGAAATTGATAGAATTTGAGCTCTACTGGTTTTTCACATTTGATCACATAACGCAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTTGTTAAGATGGTTCCTGCAGTTAGTATTATGATATTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACAAGAAGCCTACTCCATGTATGTTGACTTAACTTTTGTCTCTAATGTTGCATTGGCATTGTTTGCTCATTTTTTCTCTTTAATTGTATTCTACTGTTTTCACATTGTCTGATTAATTTTCCTGCAGAGTGATAGTAACTGGAAGAAAAGCCAGACATATAAAGTCATGACCTTGTATCTTCAACCATTATTGCTATGGACTGGAGCAACACTTATTTGCAGGTCCGTGATATATATTATTTTATATGTTGAGACGTGGACTATTCTTCTCAGTCGTACATTCAGAGATATTTTTCTCGCTAATTTAGTTTAATATTCCTGATCTTGCTTTTGCAGAGCTTTAGATCCAGTAGTTTTACCTACAGAGTCTAGCCAAGTTGTGAAGCAACGGGTGTTGAATTTTGTTAGGTCATTGTCCACTGTACTGGCCTTTGCGTATTGCTTATCTAGGTGAGTATATGAGATCGCGCCGTTTATAATGCTCAATAAGTTTACCTCTATGGTAAAGAAATCTACCATAGCTATGAACAAGATAATCTAGCCAGCAAGGATATGTTACCCTTACATGAGGCCTTTCGTTATGAATGTTAAAATACTTTAAATTCAATTTTATTAACACTTGCTTTCCTTTTCTTTTTTTTGGTTGATTGTGTAGCATGATTCAACAAGCACAGAAATTCTTCTCAGAGACTAGTGAATCCAGTGATGCGAGAAATGTATGTACAGTGTAACATCACATTTTTAGTCTATGCTTAATAGATATCATGTTATTAGGTTTCTGATGGTTAATGAACGTTTCATGTGTAGATCATTTTATGATGACTTTGTTGTAAGCTCTGAGTTGTATGGTATGTGACATTATACTTGAAGCAAGGCACCATTTGATTATCTTTTCCACGTTTCTTGTATGATGCAATTCAGCACAAGCTATAGCTGATACATGACTATATTGCTTACTTTTATTTTGAAGACAATTTGAGTGGCATCCATTATCTTATTTATCTCAATATCAGAAAGAAATTGTTCAAATAGCTTTGCATTCCTGAAATTACATACTTCAAGTACACACAATATATAGAGCTGGTGCTTTTCAGATAAACTTCGCGTTAATGTCATACTATATTGGTTTCTGTATTCTTTGTGATGCAATTCTCCCAGGAATTCTTGTTTCATGCTTTTTTTAAAACCATGGAAATGTGATATAAGTTTTCTGCTTATGTGTAGATGGGCTTTCAATTTGCTTGGAAAGCTGTGTACTCTGCAGTATGGGTTGCTGCTCTCTCATTGTTCATGGAGCTGTTAGGCTTTTCAACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTGTTGCTGACATTAGCTGGCCGCGAGGTGCTTTTCAATTATTATGGTTTGTGTTTTCTGTTTCCTTGAAGTTTATTTTAACTAATTTTAGATTTTCTCTCGCTGTTCCTAGATTTTCACCAACTTTCTCTCAAGTGTAATGATTCATGCAACCCGTCCTTTCGTTGTAAATGAGTGGATCCAAACGAGGATCGAGGGCTATGAAGTTTCTGGAACTGTTGAGGTTTGATGTTTCTATTGAACATGTACCCTAAAATGTTGATGTCAAACAAGTAATTTGGCGAACTCTAGTCATCAACTTACACCAATGTCTTAATTTAGAAGTTTTAGCGTATAGTTAGAAACATGTCTTATACGAACACGTTAGACATTTACTTCCATTTCAATCAACTGCAGCATGTAGGGTGGTGGTCGCCAACGATCATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATAAATTTACCATGAATGTCGTGAGAAATCTCAGCCAAAAAACTCACTGGCGGATTAAAACCCACCTAGCCATCAGCCATTTGGATGTCAGTAAAATCAATGTGAGAACATTTTATTTTGATCTTTTTATATGAATGTATGTATGTACGTACGTATGGTCGAATATTGTATGGTGATCGTATGTTTATCTCTCTGCAGAATATTGTCGCAGACATGCGGAAAGTCTTGGCAAAGAACCCTCAAGTTGAACAACAGAGATTGCATAGAAGAGTTTTTTTGGAAAACGTGAATCCTGAAAATCAGGCCCTTTTGGTCAGTATTAGACATTATGGCTTGAATACTATTTTTCTTTCTGAAATTTTCAATTTTTTCCTTAATGTTTGAATAATTTAAATAACCTATAACATTATAGTTATAGGTTTTGTGATGGTTGTCGAGACAAACCGATAGGATTACGCATCATTTTTTAGAAAACGTCATAAATCCTCGTTCGTTTAAATCCTTGTTGGTCATAAATCCTAATTGACTTTTGTCCTATTTTTGTTAGATTTTGATATCTTGCTTTGTCAAGACTTCACATTTTGAAGAATACCTTTGTGTGAAGGTAAATTGAACTTTTCTGTTCTTTTTTTCTATATACTTTTCATTCTTCCAAAAGAAGTTTCAGCAATTCCATTGATCCATTTTATTGGTTTGGAGCTTGTCTGAATACATTCGAGTTCTGTGTCGTTTGTTTTCGATTAACTTGTGATCTGATGAATCAGGAAGCTATATTTTTGGATCTTCTTAGAGTCATTAGACATCACCGAGCTCGACTTGCGACACCAATCCGCACAGTGCAGAAGATGCATAGTGATTCAGACTTGGAAAGCGTTCCATTTTCTGATTCAATATTTGGTCATGGTGGTGCGACTTTGAATCGTCGCATGCTGATGATTGAACCCCCCTACAAAGTTTATGGAGATGATCGAAAGCAAAGCCATAGTAGGACATCACGCACAACTGGAGAACAAAACGGTAAGCCCATTGCACGGTCATCAGGTGACAACAAGGCAGCTAAAGAGACAACACCATCGGAAAGAAAGACGGAAGTAAAGCCTGGAGAAGGACGAGACTCCGATGCAAAAATACATTCCAAAGTTCCGATGTCGACCTCTGAAGATAAACCAAGCAGTGAATCGAAACATAAATCATCATCAAGGTCTGTGTCGAGTACGAATGGTATCGCTGATATGCCAACTTCTGATGCCAAAACAACTCCATCAGATGCTGATAATTCAATGCAAGATTCTGGCTCCTCCAAGAAATCCAAAAATAGCTCAGGGAGTAACAACAAGCAGAACTACAAGCCTATCCATTCTTCTGTTTCCTTTCAGGAAGATGTCAAGAAACCAGAAGGAACTACTTCATCAGCTTCACAGCCAAGGATAGAAGGTGATCAGACGACGAATCCATCAACAACAAAGCCCGGCGTAGAAGAGAATATAATCCTCGGCGTTGCTTTGGATGGCTCTAAGAGAACACTCCCTATCGAGGACGACACGCCATCTTCCCCTCAAACGACGTTGGGAGCAAAAGATTTGGCTGCTGCATCCCTGAATGGGAACGGGGCGACTGGAGCAACAACTCCCGACAAGGAAACGAAACGGCTGTCTCCTCCTACCACATCTTCTATTGAATAATGAGACTAGTTTGATTGTTGAGTAAACAACTTTTATGACCGAGAAGTTCGGGGTGAGGCCCCGCCCCGTGAAGCCTGCATCTTCTCGGTGCTGCAACGAGTACCGTAAACGGAATTAGTGTAGAGCAGGAATGTATATTTGTATAATTTTTGACTCTTTTCAGTGAATTCTGAAACAAGAACAAGGTGATCACTTCCCGGAGCAGAGGCCATTGATGAGTTTTCTTGATATAGAAGCTTTCTTTCTGTATATAAAACCTGATCTACAAATAAGTGTGGGTTATATAATTATATAATTATATAATTTGCCCTTGGTTCAA

mRNA sequence

GAAGAAACAGAGACGGCGCCTCGATTGGAAGCTCTACAGCTTTGGGCATCAACAAAATTTGATTCCATTTCTGTAGTTTTCAGCCGTCACTGTGCTGCGTAGAAGTCTCATTGTTCAATTTTCATCCTCCCAGCATTTAGTTAGTCTTAGCAATGGTTCTTGTTGGTTCTTTGCAACTGTCCTATCATCTGGGACCGTGGAAAAACCATTTGCTTGAAGAAAATCTTAAGTTTACCTCTCATAGCAATAACATACGGTTACAGAATGTAGCCAGTCCATCGTCTCTTTTATTTCAGCAGAAGAACACTTGGAGTAGTCATTTATTTAGCTTGAAATACCCACCTAGTTACGTTGTGCCTTCTAGATACAATGCATTCAGGTGCCATTCGTCATTGATGACAAACCAACCACTGGATCCCTCTGGGATGAAGGCTGCTATAGTGGCGTTGACAAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTTGTTAAGATGGTTCCTGCAGTTAGTATTATGATATTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACAAGAAGCCTACTCCATAGTGATAGTAACTGGAAGAAAAGCCAGACATATAAAGTCATGACCTTGTATCTTCAACCATTATTGCTATGGACTGGAGCAACACTTATTTGCAGAGCTTTAGATCCAGTAGTTTTACCTACAGAGTCTAGCCAAGTTGTGAAGCAACGGGTGTTGAATTTTGTTAGGTCATTGTCCACTGTACTGGCCTTTGCGTATTGCTTATCTAGCATGATTCAACAAGCACAGAAATTCTTCTCAGAGACTAGTGAATCCAGTGATGCGAGAAATATGGGCTTTCAATTTGCTTGGAAAGCTGTGTACTCTGCAGTATGGGTTGCTGCTCTCTCATTGTTCATGGAGCTGTTAGGCTTTTCAACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTGTTGCTGACATTAGCTGGCCGCGAGATTTTCACCAACTTTCTCTCAAGTGTAATGATTCATGCAACCCGTCCTTTCGTTGTAAATGAGTGGATCCAAACGAGGATCGAGGGCTATGAAGTTTCTGGAACTGTTGAGCATGTAGGGTGGTGGTCGCCAACGATCATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATAAATTTACCATGAATGTCGTGAGAAATCTCAGCCAAAAAACTCACTGGCGGATTAAAACCCACCTAGCCATCAGCCATTTGGATGTCAGTAAAATCAATAATATTGTCGCAGACATGCGGAAAGTCTTGGCAAAGAACCCTCAAGTTGAACAACAGAGATTGCATAGAAGAGTTTTTTTGGAAAACGTGAATCCTGAAAATCAGGCCCTTTTGATTTTGATATCTTGCTTTGTCAAGACTTCACATTTTGAAGAATACCTTTGTGTGAAGGAAGCTATATTTTTGGATCTTCTTAGAGTCATTAGACATCACCGAGCTCGACTTGCGACACCAATCCGCACAGTGCAGAAGATGCATAGTGATTCAGACTTGGAAAGCGTTCCATTTTCTGATTCAATATTTGGTCATGGTGGTGCGACTTTGAATCGTCGCATGCTGATGATTGAACCCCCCTACAAAGTTTATGGAGATGATCGAAAGCAAAGCCATAGTAGGACATCACGCACAACTGGAGAACAAAACGGTAAGCCCATTGCACGGTCATCAGGTGACAACAAGGCAGCTAAAGAGACAACACCATCGGAAAGAAAGACGGAAGTAAAGCCTGGAGAAGGACGAGACTCCGATGCAAAAATACATTCCAAAGTTCCGATGTCGACCTCTGAAGATAAACCAAGCAGTGAATCGAAACATAAATCATCATCAAGGTCTGTGTCGAGTACGAATGGTATCGCTGATATGCCAACTTCTGATGCCAAAACAACTCCATCAGATGCTGATAATTCAATGCAAGATTCTGGCTCCTCCAAGAAATCCAAAAATAGCTCAGGGAGTAACAACAAGCAGAACTACAAGCCTATCCATTCTTCTGTTTCCTTTCAGGAAGATGTCAAGAAACCAGAAGGAACTACTTCATCAGCTTCACAGCCAAGGATAGAAGGTGATCAGACGACGAATCCATCAACAACAAAGCCCGGCGTAGAAGAGAATATAATCCTCGGCGTTGCTTTGGATGGCTCTAAGAGAACACTCCCTATCGAGGACGACACGCCATCTTCCCCTCAAACGACGTTGGGAGCAAAAGATTTGGCTGCTGCATCCCTGAATGGGAACGGGGCGACTGGAGCAACAACTCCCGACAAGGAAACGAAACGGCTGTCTCCTCCTACCACATCTTCTATTGAATAATGAGACTAGTTTGATTGTTGAGTAAACAACTTTTATGACCGAGAAGTTCGGGGTGAGGCCCCGCCCCGTGAAGCCTGCATCTTCTCGGTGCTGCAACGAGTACCGTAAACGGAATTAGTGTAGAGCAGGAATGTATATTTGTATAATTTTTGACTCTTTTCAGTGAATTCTGAAACAAGAACAAGGTGATCACTTCCCGGAGCAGAGGCCATTGATGAGTTTTCTTGATATAGAAGCTTTCTTTCTGTATATAAAACCTGATCTACAAATAAGTGTGGGTTATATAATTATATAATTATATAATTTGCCCTTGGTTCAA

Coding sequence (CDS)

ATGGTTCTTGTTGGTTCTTTGCAACTGTCCTATCATCTGGGACCGTGGAAAAACCATTTGCTTGAAGAAAATCTTAAGTTTACCTCTCATAGCAATAACATACGGTTACAGAATGTAGCCAGTCCATCGTCTCTTTTATTTCAGCAGAAGAACACTTGGAGTAGTCATTTATTTAGCTTGAAATACCCACCTAGTTACGTTGTGCCTTCTAGATACAATGCATTCAGGTGCCATTCGTCATTGATGACAAACCAACCACTGGATCCCTCTGGGATGAAGGCTGCTATAGTGGCGTTGACAAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTTGTTAAGATGGTTCCTGCAGTTAGTATTATGATATTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACAAGAAGCCTACTCCATAGTGATAGTAACTGGAAGAAAAGCCAGACATATAAAGTCATGACCTTGTATCTTCAACCATTATTGCTATGGACTGGAGCAACACTTATTTGCAGAGCTTTAGATCCAGTAGTTTTACCTACAGAGTCTAGCCAAGTTGTGAAGCAACGGGTGTTGAATTTTGTTAGGTCATTGTCCACTGTACTGGCCTTTGCGTATTGCTTATCTAGCATGATTCAACAAGCACAGAAATTCTTCTCAGAGACTAGTGAATCCAGTGATGCGAGAAATATGGGCTTTCAATTTGCTTGGAAAGCTGTGTACTCTGCAGTATGGGTTGCTGCTCTCTCATTGTTCATGGAGCTGTTAGGCTTTTCAACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTGTTGCTGACATTAGCTGGCCGCGAGATTTTCACCAACTTTCTCTCAAGTGTAATGATTCATGCAACCCGTCCTTTCGTTGTAAATGAGTGGATCCAAACGAGGATCGAGGGCTATGAAGTTTCTGGAACTGTTGAGCATGTAGGGTGGTGGTCGCCAACGATCATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATAAATTTACCATGAATGTCGTGAGAAATCTCAGCCAAAAAACTCACTGGCGGATTAAAACCCACCTAGCCATCAGCCATTTGGATGTCAGTAAAATCAATAATATTGTCGCAGACATGCGGAAAGTCTTGGCAAAGAACCCTCAAGTTGAACAACAGAGATTGCATAGAAGAGTTTTTTTGGAAAACGTGAATCCTGAAAATCAGGCCCTTTTGATTTTGATATCTTGCTTTGTCAAGACTTCACATTTTGAAGAATACCTTTGTGTGAAGGAAGCTATATTTTTGGATCTTCTTAGAGTCATTAGACATCACCGAGCTCGACTTGCGACACCAATCCGCACAGTGCAGAAGATGCATAGTGATTCAGACTTGGAAAGCGTTCCATTTTCTGATTCAATATTTGGTCATGGTGGTGCGACTTTGAATCGTCGCATGCTGATGATTGAACCCCCCTACAAAGTTTATGGAGATGATCGAAAGCAAAGCCATAGTAGGACATCACGCACAACTGGAGAACAAAACGGTAAGCCCATTGCACGGTCATCAGGTGACAACAAGGCAGCTAAAGAGACAACACCATCGGAAAGAAAGACGGAAGTAAAGCCTGGAGAAGGACGAGACTCCGATGCAAAAATACATTCCAAAGTTCCGATGTCGACCTCTGAAGATAAACCAAGCAGTGAATCGAAACATAAATCATCATCAAGGTCTGTGTCGAGTACGAATGGTATCGCTGATATGCCAACTTCTGATGCCAAAACAACTCCATCAGATGCTGATAATTCAATGCAAGATTCTGGCTCCTCCAAGAAATCCAAAAATAGCTCAGGGAGTAACAACAAGCAGAACTACAAGCCTATCCATTCTTCTGTTTCCTTTCAGGAAGATGTCAAGAAACCAGAAGGAACTACTTCATCAGCTTCACAGCCAAGGATAGAAGGTGATCAGACGACGAATCCATCAACAACAAAGCCCGGCGTAGAAGAGAATATAATCCTCGGCGTTGCTTTGGATGGCTCTAAGAGAACACTCCCTATCGAGGACGACACGCCATCTTCCCCTCAAACGACGTTGGGAGCAAAAGATTTGGCTGCTGCATCCCTGAATGGGAACGGGGCGACTGGAGCAACAACTCCCGACAAGGAAACGAAACGGCTGTCTCCTCCTACCACATCTTCTATTGAATAA

Protein sequence

MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTTNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDKETKRLSPPTTSSIE
Homology
BLAST of CmoCh16G008070 vs. ExPASy Swiss-Prot
Match: Q56X46 (Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL2 PE=2 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 4.6e-196
Identity = 416/733 (56.75%), Postives = 498/733 (67.94%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNI---RLQNVASPSSLLFQQKNTWSSHL 60
           M L G+LQLS+ LG      L  N +F +  N+    RL     P SL         S++
Sbjct: 1   MALYGTLQLSHSLG------LCRNQRFCNPENSAMRRRLHISNGPLSLGVPLGQHGFSNI 60

Query: 61  FSLKY--PPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVK 120
               Y   P   VP R  AFRCHS   + + ++P+ +KA  V LT+   ++    P V K
Sbjct: 61  LLSNYLRRPICSVPCRTTAFRCHSFSASGKAIEPA-VKAVTVVLTKSHGLMQQF-PFVYK 120

Query: 121 MVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLIC 180
           +VPAV++++F++WGL PF R  R++L   +D+ WKKS TY VMT Y+QPLLLW GA  IC
Sbjct: 121 LVPAVALLVFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFIC 180

Query: 181 RALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNM 240
           RALDPVVLPTE+S++VK R+LNFVRSLSTVLAFAYCLSS+IQQ QK FSETS  SD RNM
Sbjct: 181 RALDPVVLPTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNM 240

Query: 241 GFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMI 300
           GFQFA KA+YSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMI
Sbjct: 241 GFQFAGKALYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMI 300

Query: 301 HATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLS 360
           HATRPFV+NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNHKFT+NVVRNL+
Sbjct: 301 HATRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLT 360

Query: 361 QKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALL 420
           QKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL 
Sbjct: 361 QKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALS 420

Query: 421 ILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSD 480
           ILISCFVKTSH EEYL VKEAI LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +
Sbjct: 421 ILISCFVKTSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGE 480

Query: 481 SIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKET 540
           S++  GG T  R +++IEP YK+ G+D+ +S +R ++ T EQ  K      G N  +KET
Sbjct: 481 SMY--GGVTSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKET 540

Query: 541 TPSERKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSD 600
           +  + K  VK GE   SD    +KVP          E+  K   ++VS            
Sbjct: 541 SSPDLKANVKVGESPVSDT---NKVP---------EETVAKPVIKAVSK----------- 600

Query: 601 AKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPR 660
              TP D +     SG+ K     SG   K                 K + T SS S   
Sbjct: 601 -PPTPKDTET----SGTEKPKAKRSGGTIKST---------------KTDETDSSTS--- 659

Query: 661 IEGDQTTNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGN 720
                    S ++  +EENI+LGVAL+GSKRTLPIE++  S P  T  AK+L  A    +
Sbjct: 661 ---------SASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMET-DAKELTGA--RRS 659

Query: 721 GATGATTPDKETK 727
           G  G    DKE K
Sbjct: 721 GGNGPLVADKEQK 659

BLAST of CmoCh16G008070 vs. ExPASy Swiss-Prot
Match: Q8L7W1 (Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL3 PE=1 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 2.9e-174
Identity = 361/698 (51.72%), Postives = 467/698 (66.91%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           M+   +L LS+ L   K H  E +    S +   R+    +  S    +++ WS  L   
Sbjct: 2   MMRTVALPLSHDLNVHKIH--EASGFHNSAAGKNRVYLTRTGLSSCATRQDVWSLQLLES 61

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
                  V SR NAF C S+L      +   +K+  V  TR  + LGG  P +VK++PAV
Sbjct: 62  LSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG-NPHLVKLIPAV 121

Query: 121 SIMIFAVWGLGPFLRYTRSLLH---SDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALD 180
            I+ FA WGL P LR  R+ L    +D+N +KS T  ++  YLQPLLLW+GA L+CR LD
Sbjct: 122 GILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLD 181

Query: 181 PVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQF 240
           P+VLP+ + Q +KQR+L F RS+STVLAF+ CLSS++QQ QKFF ET+  +D RNMGF F
Sbjct: 182 PIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSF 241

Query: 241 AWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATR 300
           A KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATR
Sbjct: 242 AGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATR 301

Query: 301 PFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTH 360
           PFV+NEWIQT+I GYEVSGTVE VGWWSPTIIRG+DREAVHIPNH+F++N+VRNL+QKTH
Sbjct: 302 PFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTH 361

Query: 361 WRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILIS 420
           WRIKTHLAISHLDVSKINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILIS
Sbjct: 362 WRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILIS 421

Query: 421 CFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFG 480
           CFVKTS FEEYLCVKEA+ LDLL VIRHH ARLATPIRTVQ+M +++++++  FSD +F 
Sbjct: 422 CFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFN 481

Query: 481 HGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSE 540
              A +NRR ++IEP YK+  DD  +S    S + G+++  P  +S             E
Sbjct: 482 Q--AAMNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS------------EE 541

Query: 541 RKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTT 600
           R  + +P E + ++ + +  VP+S ++     +   K++  S S+T       T  + T+
Sbjct: 542 RDLQEEPSETK-AETENNGSVPVSNAK-----KENQKAALGSNSNTG------TKGSSTS 601

Query: 601 PSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGD 660
            SD   + +   S +K K S G  +K     +    +  E   K +    S         
Sbjct: 602 TSDQPVAQK---SEEKKKESVGDPHKAEKDEVSDDEATIEQTLKSKAKQGSEKNNGESKA 661

Query: 661 QTTNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSS 696
           +    S T   +EEN++LGVALDGSKRTLPI+++  +S
Sbjct: 662 RDGGGSGTSSLLEENLVLGVALDGSKRTLPIDEEHKAS 664

BLAST of CmoCh16G008070 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.2e-21
Identity = 78/293 (26.62%), Postives = 149/293 (50.85%), Query Frame = 0

Query: 170 TLICRALDPV---VLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQK-FFSETS 229
           T+I  A+  +   V+     + +   V++F+ +    +     L  +I Q +K    +  
Sbjct: 51  TIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGR 110

Query: 230 ESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 289
           + + AR M      + +   + V  + L+ E  G S    LT GG+G + + +AG++I +
Sbjct: 111 DITSARIMS-----RIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS 170

Query: 290 NFLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFT 349
           NF S +M++  RPF + +WI  R     + GTV  +G W  T I   D   +++PN  F+
Sbjct: 171 NFFSGIMLYFDRPFSIGDWI--RSPDRNIEGTVAEIG-WRITKITTFDNRPLYVPNSLFS 230

Query: 350 MNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 409
              V N  + T+ RI T + + + D +K+  IV  +R++L  +P ++Q    R+  L   
Sbjct: 231 SISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQ----RQTLLVYF 290

Query: 410 NP-ENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTV 458
           N   + +L I++ CF KT+ + E+L  ++ ++L ++ +++ H A  A P +T+
Sbjct: 291 NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of CmoCh16G008070 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.2e-21
Identity = 78/293 (26.62%), Postives = 149/293 (50.85%), Query Frame = 0

Query: 170 TLICRALDPV---VLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQK-FFSETS 229
           T+I  A+  +   V+     + +   V++F+ +    +     L  +I Q +K    +  
Sbjct: 51  TIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGR 110

Query: 230 ESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 289
           + + AR M      + +   + V  + L+ E  G S    LT GG+G + + +AG++I +
Sbjct: 111 DITSARIMS-----RIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS 170

Query: 290 NFLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFT 349
           NF S +M++  RPF + +WI  R     + GTV  +G W  T I   D   +++PN  F+
Sbjct: 171 NFFSGIMLYFDRPFSIGDWI--RSPDRNIEGTVAEIG-WRITKITTFDNRPLYVPNSLFS 230

Query: 350 MNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 409
              V N  + T+ RI T + + + D +K+  IV  +R++L  +P ++Q    R+  L   
Sbjct: 231 SISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQ----RQTLLVYF 290

Query: 410 NP-ENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTV 458
           N   + +L I++ CF KT+ + E+L  ++ ++L ++ +++ H A  A P +T+
Sbjct: 291 NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of CmoCh16G008070 vs. ExPASy Swiss-Prot
Match: Q58543 (Large-conductance mechanosensitive channel MscMJLR OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1143 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 6.3e-12
Identity = 61/250 (24.40%), Postives = 117/250 (46.80%), Query Frame = 0

Query: 180 VLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAW 239
           +LP+  + V +  +  F+  L  V+ F   L+ ++++            D  +       
Sbjct: 85  LLPSLKTAVNEGILTAFI--LCVVVFFDRFLNELVERYLALTISKKTKKDVDDQIVVLTK 144

Query: 240 KAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF 299
           K V   VWV  L L +  LG+  +  L   G+G + + LA + + +N ++ ++I   +PF
Sbjct: 145 KLVRLVVWVVGLLLILSNLGYDIKTLLAGLGIGGLAVALASQNLVSNLIAGLIILTDKPF 204

Query: 300 VVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWR 359
            +  WI T   G   SG VE +G  S T IR  D   + +PN K    +++N+  K  W+
Sbjct: 205 KIGNWI-TFSGG---SGIVEDIGIRS-TKIRATDNSIIVVPNSKLIDEIIQNVPSKNKWK 264

Query: 360 IKTHLAISH-LDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISC 419
           + T + +++   V KI      ++ +L ++P VE + +   V+ +     + +L I +  
Sbjct: 265 VSTTIGVTYNTPVEKIRKAEEIIKNILLEHPNVEDEPI--TVYFKEFG--DWSLNIQVVY 323

Query: 420 FVKTSHFEEY 429
           ++K S +  Y
Sbjct: 325 YIKNSRYNGY 323

BLAST of CmoCh16G008070 vs. ExPASy TrEMBL
Match: A0A6J1ES61 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 1425.2 bits (3688), Expect = 0.0e+00
Identity = 737/737 (100.00%), Postives = 737/737 (100.00%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360
           VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI
Sbjct: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360

Query: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420
           KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV
Sbjct: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420

Query: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480
           KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG
Sbjct: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480

Query: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540
           ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT
Sbjct: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540

Query: 541 EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD 600
           EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD
Sbjct: 541 EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD 600

Query: 601 ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT 660
           ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT
Sbjct: 601 ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT 660

Query: 661 NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT 720
           NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT
Sbjct: 661 NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT 720

Query: 721 PDKETKRLSPPTTSSIE 738
           PDKETKRLSPPTTSSIE
Sbjct: 721 PDKETKRLSPPTTSSIE 737

BLAST of CmoCh16G008070 vs. ExPASy TrEMBL
Match: A0A6J1JBP8 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483011 PE=4 SV=1)

HSP 1 Score: 1379.8 bits (3570), Expect = 0.0e+00
Identity = 718/738 (97.29%), Postives = 725/738 (98.24%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLK TSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKLTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVM+LYLQPLLLWTGATLICRALDPVV
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMSLYLQPLLLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360
           VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI
Sbjct: 301 VNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360

Query: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420
           KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV
Sbjct: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420

Query: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480
           KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG
Sbjct: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480

Query: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540
           ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQN KPIARSSGDNKAAKET+PSERK 
Sbjct: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNSKPIARSSGDNKAAKETSPSERKM 540

Query: 541 EVKPGEGRDSDAKIHSKVPMSTSED-KPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPS 600
           EVK GEGRDSD KI+SKVPMSTSED KPSSESKHKSSSRSVSSTNGIADMPTSDAKTT  
Sbjct: 541 EVKTGEGRDSDTKIYSKVPMSTSEDNKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTTL 600

Query: 601 DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT 660
           DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT
Sbjct: 601 DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT 660

Query: 661 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 720
           TNP TTKP VEENIILGVALD SKRTLPIEDDTPSSPQT+LG+KDLAAASLNGN A+GAT
Sbjct: 661 TNPPTTKPSVEENIILGVALDRSKRTLPIEDDTPSSPQTSLGSKDLAAASLNGNVASGAT 720

Query: 721 TPDKETKRLSPPTTSSIE 738
           TPDKETKR SPPTTSSIE
Sbjct: 721 TPDKETKRQSPPTTSSIE 738

BLAST of CmoCh16G008070 vs. ExPASy TrEMBL
Match: A0A6J1EN69 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 1376.3 bits (3561), Expect = 0.0e+00
Identity = 717/737 (97.29%), Postives = 717/737 (97.29%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLK                    FQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLK--------------------FQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360
           VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI
Sbjct: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360

Query: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420
           KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV
Sbjct: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420

Query: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480
           KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG
Sbjct: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480

Query: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540
           ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT
Sbjct: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540

Query: 541 EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD 600
           EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD
Sbjct: 541 EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD 600

Query: 601 ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT 660
           ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT
Sbjct: 601 ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT 660

Query: 661 NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT 720
           NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT
Sbjct: 661 NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT 717

Query: 721 PDKETKRLSPPTTSSIE 738
           PDKETKRLSPPTTSSIE
Sbjct: 721 PDKETKRLSPPTTSSIE 717

BLAST of CmoCh16G008070 vs. ExPASy TrEMBL
Match: A0A6J1J9R4 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483011 PE=4 SV=1)

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 699/738 (94.72%), Postives = 706/738 (95.66%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLK                    FQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLK--------------------FQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVM+LYLQPLLLWTGATLICRALDPVV
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMSLYLQPLLLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360
           VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI
Sbjct: 301 VNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360

Query: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420
           KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV
Sbjct: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420

Query: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480
           KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG
Sbjct: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480

Query: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540
           ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQN KPIARSSGDNKAAKET+PSERK 
Sbjct: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNSKPIARSSGDNKAAKETSPSERKM 540

Query: 541 EVKPGEGRDSDAKIHSKVPMSTSED-KPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPS 600
           EVK GEGRDSD KI+SKVPMSTSED KPSSESKHKSSSRSVSSTNGIADMPTSDAKTT  
Sbjct: 541 EVKTGEGRDSDTKIYSKVPMSTSEDNKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTTL 600

Query: 601 DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT 660
           DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT
Sbjct: 601 DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT 660

Query: 661 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 720
           TNP TTKP VEENIILGVALD SKRTLPIEDDTPSSPQT+LG+KDLAAASLNGN A+GAT
Sbjct: 661 TNPPTTKPSVEENIILGVALDRSKRTLPIEDDTPSSPQTSLGSKDLAAASLNGNVASGAT 718

Query: 721 TPDKETKRLSPPTTSSIE 738
           TPDKETKR SPPTTSSIE
Sbjct: 721 TPDKETKRQSPPTTSSIE 718

BLAST of CmoCh16G008070 vs. ExPASy TrEMBL
Match: A0A6J1EMU6 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 1260.4 bits (3260), Expect = 0.0e+00
Identity = 656/656 (100.00%), Postives = 656/656 (100.00%), Query Frame = 0

Query: 82  MTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL 141
           MTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL
Sbjct: 1   MTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL 60

Query: 142 HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLS 201
           HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLS
Sbjct: 61  HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLS 120

Query: 202 TVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFS 261
           TVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFS
Sbjct: 121 TVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFS 180

Query: 262 TQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHV 321
           TQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHV
Sbjct: 181 TQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHV 240

Query: 322 GWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADM 381
           GWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADM
Sbjct: 241 GWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADM 300

Query: 382 RKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLR 441
           RKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLR
Sbjct: 301 RKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLR 360

Query: 442 VIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDR 501
           VIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDR
Sbjct: 361 VIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDR 420

Query: 502 KQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMS 561
           KQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMS
Sbjct: 421 KQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMS 480

Query: 562 TSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSN 621
           TSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSN
Sbjct: 481 TSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSN 540

Query: 622 NKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTTNPSTTKPGVEENIILGVALDG 681
           NKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTTNPSTTKPGVEENIILGVALDG
Sbjct: 541 NKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTTNPSTTKPGVEENIILGVALDG 600

Query: 682 SKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDKETKRLSPPTTSSIE 738
           SKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDKETKRLSPPTTSSIE
Sbjct: 601 SKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDKETKRLSPPTTSSIE 656

BLAST of CmoCh16G008070 vs. NCBI nr
Match: XP_022929450.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929451.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929452.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929453.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929454.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929455.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1425.2 bits (3688), Expect = 0.0e+00
Identity = 737/737 (100.00%), Postives = 737/737 (100.00%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360
           VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI
Sbjct: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360

Query: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420
           KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV
Sbjct: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420

Query: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480
           KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG
Sbjct: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480

Query: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540
           ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT
Sbjct: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540

Query: 541 EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD 600
           EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD
Sbjct: 541 EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD 600

Query: 601 ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT 660
           ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT
Sbjct: 601 ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT 660

Query: 661 NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT 720
           NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT
Sbjct: 661 NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT 720

Query: 721 PDKETKRLSPPTTSSIE 738
           PDKETKRLSPPTTSSIE
Sbjct: 721 PDKETKRLSPPTTSSIE 737

BLAST of CmoCh16G008070 vs. NCBI nr
Match: KAG6577342.1 (Mechanosensitive ion channel protein 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 728/731 (99.59%), Postives = 728/731 (99.59%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360
           VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI
Sbjct: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360

Query: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420
           KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV
Sbjct: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420

Query: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480
           KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG
Sbjct: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480

Query: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540
           ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT
Sbjct: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540

Query: 541 EVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD 600
           EVK GEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD
Sbjct: 541 EVKTGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSD 600

Query: 601 ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT 660
           ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT
Sbjct: 601 ADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT 660

Query: 661 NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATT 720
           NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGA GATT
Sbjct: 661 NPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGANGATT 720

Query: 721 PDKETKRLSPP 732
           PDKETKR SPP
Sbjct: 721 PDKETKRQSPP 731

BLAST of CmoCh16G008070 vs. NCBI nr
Match: XP_023552794.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552796.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552797.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552798.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552799.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552800.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 721/738 (97.70%), Postives = 728/738 (98.64%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRL NVASPSSLLFQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLLNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDP GMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPPGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIF+VWG+GPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV
Sbjct: 121 SIMIFSVWGIGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360
           VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI
Sbjct: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360

Query: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420
           KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV
Sbjct: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420

Query: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480
           KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLES+PFSDSIFGHGG
Sbjct: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESIPFSDSIFGHGG 480

Query: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSR-TTGEQNGKPIARSSGDNKAAKETTPSERK 540
           ATLNRRMLMIEPPYK+YGDDRKQSH RTSR TTGEQN KPIARSSGDNKA+KETTPSERK
Sbjct: 481 ATLNRRMLMIEPPYKIYGDDRKQSHGRTSRTTTGEQNSKPIARSSGDNKASKETTPSERK 540

Query: 541 TEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPS 600
           TEVK GEGRDSD KIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMP+SDAKTT S
Sbjct: 541 TEVKTGEGRDSDTKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPSSDAKTTTS 600

Query: 601 DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT 660
           D DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT
Sbjct: 601 DTDNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT 660

Query: 661 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 720
           TNPSTTKPGVEENIILGVALDGSKRTLPI+DDTPSSPQTTLGAKDLAAASLNGNGATGAT
Sbjct: 661 TNPSTTKPGVEENIILGVALDGSKRTLPIDDDTPSSPQTTLGAKDLAAASLNGNGATGAT 720

Query: 721 TPDKETKRLSPPTTSSIE 738
           TPDKETKR SPPTTSSIE
Sbjct: 721 TPDKETKRQSPPTTSSIE 738

BLAST of CmoCh16G008070 vs. NCBI nr
Match: KAG7015430.1 (Mechanosensitive ion channel protein 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 728/759 (95.92%), Postives = 729/759 (96.05%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPL LWTGATLICRALDPVV
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLFLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVE------------------HVGWWSPTIIRGEDREAVHIPNH 360
           VNEWIQTRIEGYEVSGTVE                  HVGWWSPTIIRGEDREAVHIPNH
Sbjct: 301 VNEWIQTRIEGYEVSGTVEKHVLYEHVRHYFHFNQLQHVGWWSPTIIRGEDREAVHIPNH 360

Query: 361 KFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFL 420
           KFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFL
Sbjct: 361 KFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFL 420

Query: 421 ENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHS 480
           ENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHS
Sbjct: 421 ENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHS 480

Query: 481 DSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSR-TTGEQNGKPIA 540
           DSDLESVPFSDSIFGHGGATLNRRMLMIEPPYK+YGDDRKQSHSRTSR TTGEQN KPIA
Sbjct: 481 DSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKIYGDDRKQSHSRTSRTTTGEQNSKPIA 540

Query: 541 RSSGDNKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVS 600
           RSSGDNKAAKETTPSERKTEVK GEGRDSD KIHSKVPMSTSEDKPSSESKHKSSSRSVS
Sbjct: 541 RSSGDNKAAKETTPSERKTEVKTGEGRDSDTKIHSKVPMSTSEDKPSSESKHKSSSRSVS 600

Query: 601 --STNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDV 660
             STNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDV
Sbjct: 601 STSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDV 660

Query: 661 KKPEGTTSSASQPRIEGDQTTNPSTTKP-GVEENIILGVALDGSKRTLPIEDDTPSSPQT 720
           KKPEGTTS ASQPRIEGDQTTNPSTTK  GVEENIILGVALDGSKRTLPIEDDTPSSPQT
Sbjct: 661 KKPEGTTSPASQPRIEGDQTTNPSTTKTGGVEENIILGVALDGSKRTLPIEDDTPSSPQT 720

Query: 721 TLGAKDLAAASLNGNGATGATTPDKETKRLSPPTTSSIE 738
           TLGAKDLAAASLNGNGA GATTPDKETKR SPPTTSSIE
Sbjct: 721 TLGAKDLAAASLNGNGANGATTPDKETKRQSPPTTSSIE 759

BLAST of CmoCh16G008070 vs. NCBI nr
Match: XP_022984854.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022984855.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022984856.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022984857.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022984859.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1379.8 bits (3570), Expect = 0.0e+00
Identity = 718/738 (97.29%), Postives = 725/738 (98.24%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           MVLVGSLQLSYHLGPWKNHLLEENLK TSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKLTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
           KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVV 180
           SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVM+LYLQPLLLWTGATLICRALDPVV
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLLHSDSNWKKSQTYKVMSLYLQPLLLWTGATLICRALDPVV 180

Query: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240
           LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK
Sbjct: 181 LPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWK 240

Query: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300
           AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV
Sbjct: 241 AVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFV 300

Query: 301 VNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360
           VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI
Sbjct: 301 VNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRI 360

Query: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420
           KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV
Sbjct: 361 KTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFV 420

Query: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480
           KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG
Sbjct: 421 KTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGG 480

Query: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKT 540
           ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQN KPIARSSGDNKAAKET+PSERK 
Sbjct: 481 ATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNSKPIARSSGDNKAAKETSPSERKM 540

Query: 541 EVKPGEGRDSDAKIHSKVPMSTSED-KPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPS 600
           EVK GEGRDSD KI+SKVPMSTSED KPSSESKHKSSSRSVSSTNGIADMPTSDAKTT  
Sbjct: 541 EVKTGEGRDSDTKIYSKVPMSTSEDNKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTTL 600

Query: 601 DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT 660
           DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT
Sbjct: 601 DADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT 660

Query: 661 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 720
           TNP TTKP VEENIILGVALD SKRTLPIEDDTPSSPQT+LG+KDLAAASLNGN A+GAT
Sbjct: 661 TNPPTTKPSVEENIILGVALDRSKRTLPIEDDTPSSPQTSLGSKDLAAASLNGNVASGAT 720

Query: 721 TPDKETKRLSPPTTSSIE 738
           TPDKETKR SPPTTSSIE
Sbjct: 721 TPDKETKRQSPPTTSSIE 738

BLAST of CmoCh16G008070 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 686.0 bits (1769), Expect = 3.3e-197
Identity = 416/733 (56.75%), Postives = 498/733 (67.94%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNI---RLQNVASPSSLLFQQKNTWSSHL 60
           M L G+LQLS+ LG      L  N +F +  N+    RL     P SL         S++
Sbjct: 1   MALYGTLQLSHSLG------LCRNQRFCNPENSAMRRRLHISNGPLSLGVPLGQHGFSNI 60

Query: 61  FSLKY--PPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVK 120
               Y   P   VP R  AFRCHS   + + ++P+ +KA  V LT+   ++    P V K
Sbjct: 61  LLSNYLRRPICSVPCRTTAFRCHSFSASGKAIEPA-VKAVTVVLTKSHGLMQQF-PFVYK 120

Query: 121 MVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLIC 180
           +VPAV++++F++WGL PF R  R++L   +D+ WKKS TY VMT Y+QPLLLW GA  IC
Sbjct: 121 LVPAVALLVFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFIC 180

Query: 181 RALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNM 240
           RALDPVVLPTE+S++VK R+LNFVRSLSTVLAFAYCLSS+IQQ QK FSETS  SD RNM
Sbjct: 181 RALDPVVLPTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNM 240

Query: 241 GFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMI 300
           GFQFA KA+YSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMI
Sbjct: 241 GFQFAGKALYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMI 300

Query: 301 HATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLS 360
           HATRPFV+NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNHKFT+NVVRNL+
Sbjct: 301 HATRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLT 360

Query: 361 QKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALL 420
           QKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL 
Sbjct: 361 QKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALS 420

Query: 421 ILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSD 480
           ILISCFVKTSH EEYL VKEAI LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +
Sbjct: 421 ILISCFVKTSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGE 480

Query: 481 SIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKET 540
           S++  GG T  R +++IEP YK+ G+D+ +S +R ++ T EQ  K      G N  +KET
Sbjct: 481 SMY--GGVTSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKET 540

Query: 541 TPSERKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSD 600
           +  + K  VK GE   SD    +KVP          E+  K   ++VS            
Sbjct: 541 SSPDLKANVKVGESPVSDT---NKVP---------EETVAKPVIKAVSK----------- 600

Query: 601 AKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPR 660
              TP D +     SG+ K     SG   K                 K + T SS S   
Sbjct: 601 -PPTPKDTET----SGTEKPKAKRSGGTIKST---------------KTDETDSSTS--- 659

Query: 661 IEGDQTTNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGN 720
                    S ++  +EENI+LGVAL+GSKRTLPIE++  S P  T  AK+L  A    +
Sbjct: 661 ---------SASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMET-DAKELTGA--RRS 659

Query: 721 GATGATTPDKETK 727
           G  G    DKE K
Sbjct: 721 GGNGPLVADKEQK 659

BLAST of CmoCh16G008070 vs. TAIR 10
Match: AT5G10490.3 (MSCS-like 2 )

HSP 1 Score: 679.9 bits (1753), Expect = 2.3e-195
Identity = 408/723 (56.43%), Postives = 490/723 (67.77%), Query Frame = 0

Query: 24  NLKFTSHSNNI-RLQNVASPSSLLFQQKNTWSSHLFSLKYP-----------------PS 83
           N  F SHS  + R Q   +P +   +++   S+   SL  P                 P 
Sbjct: 3   NFNFLSHSLGLCRNQRFCNPENSAMRRRLHISNGPLSLGVPLGQHGFSNILLSNYLRRPI 62

Query: 84  YVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAVSIMIF 143
             VP R  AFRCHS   + + ++P+ +KA  V LT+   ++    P V K+VPAV++++F
Sbjct: 63  CSVPCRTTAFRCHSFSASGKAIEPA-VKAVTVVLTKSHGLMQQF-PFVYKLVPAVALLVF 122

Query: 144 AVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVLPT 203
           ++WGL PF R  R++L   +D+ WKKS TY VMT Y+QPLLLW GA  ICRALDPVVLPT
Sbjct: 123 SLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVLPT 182

Query: 204 ESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKAVY 263
           E+S++VK R+LNFVRSLSTVLAFAYCLSS+IQQ QK FSETS  SD RNMGFQFA KA+Y
Sbjct: 183 EASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKALY 242

Query: 264 SAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNE 323
           SAVWVAA+SLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPFV+NE
Sbjct: 243 SAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVLNE 302

Query: 324 WIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTH 383
           WIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNHKFT+NVVRNL+QKTHWRIKTH
Sbjct: 303 WIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTH 362

Query: 384 LAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTS 443
           LAISHLDV+KINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVKTS
Sbjct: 363 LAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTS 422

Query: 444 HFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATL 503
           H EEYL VKEAI LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GG T 
Sbjct: 423 HHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGVTS 482

Query: 504 NRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVK 563
            R +++IEP YK+ G+D+ +S +R ++ T EQ  K      G N  +KET+  + K  VK
Sbjct: 483 RRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKANVK 542

Query: 564 PGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADN 623
            GE   SD    +KVP          E+  K   ++VS               TP D + 
Sbjct: 543 VGESPVSDT---NKVP---------EETVAKPVIKAVSK------------PPTPKDTET 602

Query: 624 SMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTTNPS 683
               SG+ K     SG   K                 K + T SS S            S
Sbjct: 603 ----SGTEKPKAKRSGGTIKST---------------KTDETDSSTS------------S 657

Query: 684 TTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDK 727
            ++  +EENI+LGVAL+GSKRTLPIE++  S P  T  AK+L  A    +G  G    DK
Sbjct: 663 ASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMET-DAKELTGA--RRSGGNGPLVADK 657

BLAST of CmoCh16G008070 vs. TAIR 10
Match: AT5G10490.2 (MSCS-like 2 )

HSP 1 Score: 679.1 bits (1751), Expect = 4.0e-195
Identity = 396/665 (59.55%), Postives = 471/665 (70.83%), Query Frame = 0

Query: 64  PSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAVSIM 123
           P   VP R  AFRCHS   + + ++P+ +KA  V LT+   ++    P V K+VPAV+++
Sbjct: 35  PICSVPCRTTAFRCHSFSASGKAIEPA-VKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 94

Query: 124 IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 183
           +F++WGL PF R  R++L   +D+ WKKS TY VMT Y+QPLLLW GA  ICRALDPVVL
Sbjct: 95  VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 154

Query: 184 PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 243
           PTE+S++VK R+LNFVRSLSTVLAFAYCLSS+IQQ QK FSETS  SD RNMGFQFA KA
Sbjct: 155 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 214

Query: 244 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 303
           +YSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPFV+
Sbjct: 215 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 274

Query: 304 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 363
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNHKFT+NVVRNL+QKTHWRIK
Sbjct: 275 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 334

Query: 364 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 423
           THLAISHLDV+KINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 335 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 394

Query: 424 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 483
           TSH EEYL VKEAI LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GG 
Sbjct: 395 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 454

Query: 484 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 543
           T  R +++IEP YK+ G+D+ +S +R ++ T EQ  K      G N  +KET+  + K  
Sbjct: 455 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 514

Query: 544 VKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 603
           VK GE   SD    +KVP          E+  K   ++VS               TP D 
Sbjct: 515 VKVGESPVSDT---NKVP---------EETVAKPVIKAVSK------------PPTPKDT 574

Query: 604 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTTN 663
           +     SG+ K     SG   K                 K + T SS S           
Sbjct: 575 ET----SGTEKPKAKRSGGTIKST---------------KTDETDSSTS----------- 631

Query: 664 PSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTP 723
            S ++  +EENI+LGVAL+GSKRTLPIE++  S P  T  AK+L  A    +G  G    
Sbjct: 635 -SASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMET-DAKELTGA--RRSGGNGPLVA 631

Query: 724 DKETK 727
           DKE K
Sbjct: 695 DKEQK 631

BLAST of CmoCh16G008070 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 613.6 bits (1581), Expect = 2.1e-175
Identity = 361/698 (51.72%), Postives = 467/698 (66.91%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           M+   +L LS+ L   K H  E +    S +   R+    +  S    +++ WS  L   
Sbjct: 2   MMRTVALPLSHDLNVHKIH--EASGFHNSAAGKNRVYLTRTGLSSCATRQDVWSLQLLES 61

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
                  V SR NAF C S+L      +   +K+  V  TR  + LGG  P +VK++PAV
Sbjct: 62  LSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG-NPHLVKLIPAV 121

Query: 121 SIMIFAVWGLGPFLRYTRSLLH---SDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALD 180
            I+ FA WGL P LR  R+ L    +D+N +KS T  ++  YLQPLLLW+GA L+CR LD
Sbjct: 122 GILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLD 181

Query: 181 PVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQF 240
           P+VLP+ + Q +KQR+L F RS+STVLAF+ CLSS++QQ QKFF ET+  +D RNMGF F
Sbjct: 182 PIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSF 241

Query: 241 AWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATR 300
           A KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATR
Sbjct: 242 AGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATR 301

Query: 301 PFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTH 360
           PFV+NEWIQT+I GYEVSGTVE VGWWSPTIIRG+DREAVHIPNH+F++N+VRNL+QKTH
Sbjct: 302 PFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTH 361

Query: 361 WRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILIS 420
           WRIKTHLAISHLDVSKINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILIS
Sbjct: 362 WRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILIS 421

Query: 421 CFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFG 480
           CFVKTS FEEYLCVKEA+ LDLL VIRHH ARLATPIRTVQ+M +++++++  FSD +F 
Sbjct: 422 CFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFN 481

Query: 481 HGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSE 540
              A +NRR ++IEP YK+  DD  +S    S + G+++  P  +S             E
Sbjct: 482 Q--AAMNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS------------EE 541

Query: 541 RKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTT 600
           R  + +P E + ++ + +  VP+S ++     +   K++  S S+T       T  + T+
Sbjct: 542 RDLQEEPSETK-AETENNGSVPVSNAK-----KENQKAALGSNSNTG------TKGSSTS 601

Query: 601 PSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGD 660
            SD   + +   S +K K S G  +K     +    +  E   K +    S         
Sbjct: 602 TSDQPVAQK---SEEKKKESVGDPHKAEKDEVSDDEATIEQTLKSKAKQGSEKNNGESKA 661

Query: 661 QTTNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSS 696
           +    S T   +EEN++LGVALDGSKRTLPI+++  +S
Sbjct: 662 RDGGGSGTSSLLEENLVLGVALDGSKRTLPIDEEHKAS 664

BLAST of CmoCh16G008070 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 613.6 bits (1581), Expect = 2.1e-175
Identity = 361/698 (51.72%), Postives = 467/698 (66.91%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60
           M+   +L LS+ L   K H  E +    S +   R+    +  S    +++ WS  L   
Sbjct: 2   MMRTVALPLSHDLNVHKIH--EASGFHNSAAGKNRVYLTRTGLSSCATRQDVWSLQLLES 61

Query: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120
                  V SR NAF C S+L      +   +K+  V  TR  + LGG  P +VK++PAV
Sbjct: 62  LSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG-NPHLVKLIPAV 121

Query: 121 SIMIFAVWGLGPFLRYTRSLLH---SDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALD 180
            I+ FA WGL P LR  R+ L    +D+N +KS T  ++  YLQPLLLW+GA L+CR LD
Sbjct: 122 GILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLD 181

Query: 181 PVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQF 240
           P+VLP+ + Q +KQR+L F RS+STVLAF+ CLSS++QQ QKFF ET+  +D RNMGF F
Sbjct: 182 PIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSF 241

Query: 241 AWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATR 300
           A KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATR
Sbjct: 242 AGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATR 301

Query: 301 PFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTH 360
           PFV+NEWIQT+I GYEVSGTVE VGWWSPTIIRG+DREAVHIPNH+F++N+VRNL+QKTH
Sbjct: 302 PFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTH 361

Query: 361 WRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILIS 420
           WRIKTHLAISHLDVSKINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILIS
Sbjct: 362 WRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILIS 421

Query: 421 CFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFG 480
           CFVKTS FEEYLCVKEA+ LDLL VIRHH ARLATPIRTVQ+M +++++++  FSD +F 
Sbjct: 422 CFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFN 481

Query: 481 HGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSE 540
              A +NRR ++IEP YK+  DD  +S    S + G+++  P  +S             E
Sbjct: 482 Q--AAMNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS------------EE 541

Query: 541 RKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTT 600
           R  + +P E + ++ + +  VP+S ++     +   K++  S S+T       T  + T+
Sbjct: 542 RDLQEEPSETK-AETENNGSVPVSNAK-----KENQKAALGSNSNTG------TKGSSTS 601

Query: 601 PSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGD 660
            SD   + +   S +K K S G  +K     +    +  E   K +    S         
Sbjct: 602 TSDQPVAQK---SEEKKKESVGDPHKAEKDEVSDDEATIEQTLKSKAKQGSEKNNGESKA 661

Query: 661 QTTNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSS 696
           +    S T   +EEN++LGVALDGSKRTLPI+++  +S
Sbjct: 662 RDGGGSGTSSLLEENLVLGVALDGSKRTLPIDEEHKAS 664

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56X464.6e-19656.75Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX... [more]
Q8L7W12.9e-17451.72Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX... [more]
P0AEB61.2e-2126.62Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833... [more]
P0AEB51.2e-2126.62Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O... [more]
Q585436.3e-1224.40Large-conductance mechanosensitive channel MscMJLR OS=Methanocaldococcus jannasc... [more]
Match NameE-valueIdentityDescription
A0A6J1ES610.0e+00100.00mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbi... [more]
A0A6J1JBP80.0e+0097.29mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbi... [more]
A0A6J1EN690.0e+0097.29mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbi... [more]
A0A6J1J9R40.0e+0094.72mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbi... [more]
A0A6J1EMU60.0e+00100.00mechanosensitive ion channel protein 2, chloroplastic-like isoform X3 OS=Cucurbi... [more]
Match NameE-valueIdentityDescription
XP_022929450.10.0e+00100.00mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita... [more]
KAG6577342.10.0e+0099.59Mechanosensitive ion channel protein 2, chloroplastic, partial [Cucurbita argyro... [more]
XP_023552794.10.0e+0097.70mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita... [more]
KAG7015430.10.0e+0095.92Mechanosensitive ion channel protein 2, chloroplastic [Cucurbita argyrosperma su... [more]
XP_022984854.10.0e+0097.29mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita... [more]
Match NameE-valueIdentityDescription
AT5G10490.13.3e-19756.75MSCS-like 2 [more]
AT5G10490.32.3e-19556.43MSCS-like 2 [more]
AT5G10490.24.0e-19559.55MSCS-like 2 [more]
AT1G58200.12.1e-17551.72MSCS-like 3 [more]
AT1G58200.22.1e-17551.72MSCS-like 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 241..447
e-value: 1.5E-29
score: 103.0
NoneNo IPR availableGENE3D1.10.287.1260coord: 193..297
e-value: 2.2E-7
score: 33.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 496..737
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 712..737
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 574..668
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 509..530
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 531..573
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 688..704
NoneNo IPR availablePANTHERPTHR43634:SF6MECHANOSENSITIVE ION CHANNEL PROTEIN 2, CHLOROPLASTICcoord: 1..726
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 298..351
e-value: 4.7E-6
score: 28.4
IPR045042Mechanosensitive ion channel protein YnaI-likePANTHERPTHR43634OW CONDUCTANCE MECHANOSENSITIVE CHANNELcoord: 1..726
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 283..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G008070.1CmoCh16G008070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane