CmoCh13G008850 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G008850
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCmo_Chr13: 7988958 .. 7990261 (-)
RNA-Seq ExpressionCmoCh13G008850
SyntenyCmoCh13G008850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGGGTTTATGATGAACTCACGTGGGTTGAAGCTTTTCACGTGCGAATGGCTTCCAAAGGATAAGGAACCAAAGGGCTTGATCTTTATCTGCCATGGGTACGCGATGGAATGCAGCATCACCATGAACAGTAAACACCCTTCCACCACCTGAAACATTATATTCCTAAGATCAAATTATAATTGATTTGGTTTATAGGCACTGCAATTCGGCTTGCAAGGGCAGGGTTTGCGGTTTATGGGATTGATTACGAAGGCCATGGGAAATCAGATGGGCTGCAAGGCTATATTACAAGCTTTGATCATGTGGTGGAGGATTGCTCCAATCACTTCACAGCCACTTCTGGTATCTTATCTTCCCAATGCAAAAGATTCCATTTTTATGGGTTTTAAGTGAAATTTTTTACATGGGTCTTCGTTAAAAACTATGTTTTTTAATACCCAGAAAGGAAAGAGAACATGGGCAAAAGAAGGTATCTATTGGGGGAGTCAATGGGAGGGGCAGTGGCTCTGTTATTGCACAGAAAGAAGCCAGATTATTGGGATGGCGCTGTCTTGGTTGCACCAATGTGTAAAGTATGCCCATGTCTTGGCTCTGTTTTTGAATGAGTTTAAACTTTGTAGCTCAAAAGATTCCCCTGTTTTTCGCAGATTGCAGAGGATGTTAAACCAAAGCCAATTGTTGTAAATATACTGGCAAAGCTTTGCAAAGTGATACCCACATGGAAGATAATTCCAACCCAAGATATCATTGATGTTGCTTTCAAAGTTCCTGAGATTAGACATCAGGTAATAATTATTAATCTCCTCTGTTTTTACAATGTAAAACTGTTTATATTAATGATAATGAAATCAACAGGTCAGAAATAATCCTTATTGTTTCAAAGGGAGGCCTCGTTTGAACACTGGAAATGAACTTCTAAGGATCAGTTTAGATCTCGAGAAACGTCTCGAAGAGGTTTGCTATTTCTTCGTTGTTTGAATGAATTGATCCTTTTCGAGCTTTTGAGTTCAGTGGTAGTTTAATAGGTTTCGTTACCGTTCATAATCCTCCATGGGGAGGTGGATCGAGTGACCGATATATCGGTGAGCAAGCAACTCCTTGAGAAGGCGTCGAGCTGGGACAAGAGCTTGAAGACATATCCAGAGATGTGGCATGGATTGCTGTATGGAGAGACAGTTGAAAACATTGATGTTGTGTTTGCTGACATAATTGGTTGGTTGGAAGAAAGATGTGGTTTGGGAAATTCAAGGATTGAGAAACAGCTCAAGACAGAATCTGATGATCTTCAGACCAAATAA

mRNA sequence

ATGCAGGGGTTTATGATGAACTCACGTGGGTTGAAGCTTTTCACGTGCGAATGGCTTCCAAAGGATAAGGAACCAAAGGGCTTGATCTTTATCTGCCATGGGTACGCGATGGAATGCAGCATCACCATGAACAGCACTGCAATTCGGCTTGCAAGGGCAGGGTTTGCGGTTTATGGGATTGATTACGAAGGCCATGGGAAATCAGATGGGCTGCAAGGCTATATTACAAGCTTTGATCATGTGGTGGAGGATTGCTCCAATCACTTCACAGCCACTTCTGAAAGGAAAGAGAACATGGGCAAAAGAAGGTATCTATTGGGGGAGTCAATGGGAGGGGCAGTGGCTCTGTTATTGCACAGAAAGAAGCCAGATTATTGGGATGGCGCTGTCTTGGTTGCACCAATGTGTAAAATTGCAGAGGATGTTAAACCAAAGCCAATTGTTGTAAATATACTGGCAAAGCTTTGCAAAGTGATACCCACATGGAAGATAATTCCAACCCAAGATATCATTGATGTTGCTTTCAAAGTTCCTGAGATTAGACATCAGGTCAGAAATAATCCTTATTGTTTCAAAGGGAGGCCTCGTTTGAACACTGGAAATGAACTTCTAAGGATCAGTTTAGATCTCGAGAAACGTCTCGAAGAGGTTTCGTTACCGTTCATAATCCTCCATGGGGAGGTGGATCGAGTGACCGATATATCGGTGAGCAAGCAACTCCTTGAGAAGGCGTCGAGCTGGGACAAGAGCTTGAAGACATATCCAGAGATGTGGCATGGATTGCTGTATGGAGAGACAGTTGAAAACATTGATGTTGTGTTTGCTGACATAATTGGTTGGTTGGAAGAAAGATGTGGTTTGGGAAATTCAAGGATTGAGAAACAGCTCAAGACAGAATCTGATGATCTTCAGACCAAATAA

Coding sequence (CDS)

ATGCAGGGGTTTATGATGAACTCACGTGGGTTGAAGCTTTTCACGTGCGAATGGCTTCCAAAGGATAAGGAACCAAAGGGCTTGATCTTTATCTGCCATGGGTACGCGATGGAATGCAGCATCACCATGAACAGCACTGCAATTCGGCTTGCAAGGGCAGGGTTTGCGGTTTATGGGATTGATTACGAAGGCCATGGGAAATCAGATGGGCTGCAAGGCTATATTACAAGCTTTGATCATGTGGTGGAGGATTGCTCCAATCACTTCACAGCCACTTCTGAAAGGAAAGAGAACATGGGCAAAAGAAGGTATCTATTGGGGGAGTCAATGGGAGGGGCAGTGGCTCTGTTATTGCACAGAAAGAAGCCAGATTATTGGGATGGCGCTGTCTTGGTTGCACCAATGTGTAAAATTGCAGAGGATGTTAAACCAAAGCCAATTGTTGTAAATATACTGGCAAAGCTTTGCAAAGTGATACCCACATGGAAGATAATTCCAACCCAAGATATCATTGATGTTGCTTTCAAAGTTCCTGAGATTAGACATCAGGTCAGAAATAATCCTTATTGTTTCAAAGGGAGGCCTCGTTTGAACACTGGAAATGAACTTCTAAGGATCAGTTTAGATCTCGAGAAACGTCTCGAAGAGGTTTCGTTACCGTTCATAATCCTCCATGGGGAGGTGGATCGAGTGACCGATATATCGGTGAGCAAGCAACTCCTTGAGAAGGCGTCGAGCTGGGACAAGAGCTTGAAGACATATCCAGAGATGTGGCATGGATTGCTGTATGGAGAGACAGTTGAAAACATTGATGTTGTGTTTGCTGACATAATTGGTTGGTTGGAAGAAAGATGTGGTTTGGGAAATTCAAGGATTGAGAAACAGCTCAAGACAGAATCTGATGATCTTCAGACCAAATAA

Protein sequence

MQGFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK
Homology
BLAST of CmoCh13G008850 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.4e-42
Identity = 96/274 (35.04%), Postives = 146/274 (53.28%), Query Frame = 0

Query: 12  KLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGHGKSDGL 71
           KLFT  +LP D E KG +++ HGY  + S       +  +  G+AV+  D  GHG+SDG+
Sbjct: 50  KLFTQSFLPLDGEIKGTVYMSHGYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGI 109

Query: 72  QGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLH-RKKPDYWDGAV 131
           + Y+   + V       F              +L GESMGG V LL++ + +P+ W G +
Sbjct: 110 RCYMGDMEKVAATSLAFFKHVRCSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLM 169

Query: 132 LVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYC 191
             AP+  I ED+KP    +     L  +  TW  +P   ++  A K PE    + +NP  
Sbjct: 170 FSAPLFVIPEDMKPSKAHLFAYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQR 229

Query: 192 FKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKASSWDKS 251
           + G+PR+ T  ELLR +  +++   +V++P    HG  D VT  + SK L EKASS DK+
Sbjct: 230 YTGKPRVGTMRELLRKTQYVQENFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKT 289

Query: 252 LKTYPEMWHGLLYGETVENIDVVFADIIGWLEER 285
           LK Y  M+H L+ GE  EN ++V  D+  W++E+
Sbjct: 290 LKIYEGMYHSLIQGEPDENAEIVLKDMREWIDEK 323

BLAST of CmoCh13G008850 vs. ExPASy Swiss-Prot
Match: O07427 (Monoacylglycerol lipase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv0183 PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-25
Identity = 81/268 (30.22%), Postives = 127/268 (47.39%), Query Frame = 0

Query: 18  WLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGHGKSDGLQGYITS 77
           W P D  P+ ++ + HG   E +   +  A RL  AG   Y +D+ GHG+S G +  +  
Sbjct: 23  WTP-DTAPQAVVVLAHGLG-EHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKRVLVRD 82

Query: 78  FDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCK 137
                 D        +  +E  G +R +LG SMGG +      ++PD +D  VL AP   
Sbjct: 83  ISEYTADFDTLVGIAT--REYPGCKRIVLGHSMGGGIVFAYGVERPDNYDLMVLSAPAVA 142

Query: 138 IAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAF-KVPEIRHQVRNNPYCFKGRPR 197
             + V P   VV + AKL  V+     +P Q++   A  + PE+      +P    GR  
Sbjct: 143 AQDLVSP---VVAVAAKLLGVVVPG--LPVQELDFTAISRDPEVVQAYNTDPLVHHGRVP 202

Query: 198 LNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKASSWDKSLKTYPE 257
              G  LL++   + +R   ++ P ++LHG  DR+  I  S++L+E   S D  LK YP 
Sbjct: 203 AGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVECVGSADVQLKEYPG 262

Query: 258 MWHGLLYGETVENIDVVFADIIGWLEER 285
           ++H  ++ E   N   V  D++ WL ER
Sbjct: 263 LYHE-VFNEPERN--QVLDDVVAWLTER 278

BLAST of CmoCh13G008850 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 8.7e-24
Identity = 85/280 (30.36%), Postives = 140/280 (50.00%), Query Frame = 0

Query: 5   MMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEG 64
           ++N+ G  LF   W P    PK LIF+ HG A E S      A  L      V+  D+ G
Sbjct: 22  LVNADGQYLFCRYWKPTG-TPKALIFVSHG-AGEHSGRYEELARMLMGLDLLVFAHDHVG 81

Query: 65  HGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPD 124
           HG+S+G +  ++ F   V D   H    S +K+  G   +LLG SMGGA+A+L   ++P 
Sbjct: 82  HGQSEGERMVVSDFHVFVRDVLQH--VDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPG 141

Query: 125 YWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQV 184
           ++ G VL++P+  +A         V     L  V+P   + P    + ++    E+    
Sbjct: 142 HFAGMVLISPLV-LANPESATTFKVLAAKVLNLVLPNLSLGPIDSSV-LSRNKTEV-DIY 201

Query: 185 RNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKA 244
            ++P   +   ++  G +LL     +E+ L ++++PF++L G  DR+ D   +  L+E A
Sbjct: 202 NSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELA 261

Query: 245 SSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEER 285
            S DK+LK Y   +H +L+ E  E  + VF +I  W+ +R
Sbjct: 262 KSQDKTLKIYEGAYH-VLHKELPEVTNSVFHEINMWVSQR 293

BLAST of CmoCh13G008850 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.3e-23
Identity = 70/273 (25.64%), Postives = 127/273 (46.52%), Query Frame = 0

Query: 10  GLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGHGKSD 69
           G+++    W P D +P+G++ + HGYA E +   +  A R   AG  VY +D+ GHG+S 
Sbjct: 16  GVRIVYDVWTP-DTDPRGVVVLAHGYA-EHAGRYHHVAQRFGAAGLLVYALDHRGHGRSG 75

Query: 70  GLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGA 129
           G + ++      VED        +     +   R +LG SMGG +      + P  +   
Sbjct: 76  GKRVHLRDLSEFVEDFRTLVGIAANDHPTL--PRIVLGHSMGGGIVFAYGARYPGEYSAM 135

Query: 130 VLVAPMCKIAEDVKPKPI-VVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNP 189
           VL  P     + V P  + V  +L KL   IP   +       D   + PE+    + +P
Sbjct: 136 VLSGPAVNAHDGVSPVLVAVAKVLGKLAPGIPVENL-----DADAVSRDPEVVAAYKADP 195

Query: 190 YCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKASSWD 249
               G+        L+ +   + +R   ++ P +++HG+ DR+  ++ S+ L+++ +S D
Sbjct: 196 MVHHGKLPAGIARALIGLGQSMPQRAAALTAPLLVVHGDKDRLIPVAGSRLLVDRVASED 255

Query: 250 KSLKTYPEMWHGLLYGETVENIDVVFADIIGWL 282
             LK YP ++H  ++ E  + +  V  D+  W+
Sbjct: 256 VHLKVYPGLYHE-VFNEPEQKL--VLDDVTSWI 276

BLAST of CmoCh13G008850 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.6e-23
Identity = 78/287 (27.18%), Postives = 139/287 (48.43%), Query Frame = 0

Query: 5   MMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEG 64
           ++N+ G  LF   W P    PK LIF+ HG    C    +  A  L R    V+  D+ G
Sbjct: 22  LVNADGQYLFCRYWKPSG-TPKALIFVSHGAGEHCG-RYDELAQMLKRLDMLVFAHDHVG 81

Query: 65  HGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPD 124
           HG+S+G +  ++ F   V D   H    + +K+      +LLG SMGGA+++L   ++P 
Sbjct: 82  HGQSEGERMVVSDFQVFVRDLLQH--VNTVQKDYPEVPVFLLGHSMGGAISILAAAERPT 141

Query: 125 YWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQV 184
           ++ G +L++P+  I  + +    +  + AKL   +     +   D   ++    E+    
Sbjct: 142 HFSGMILISPL--ILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLSRNKSEV-DLY 201

Query: 185 RNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKA 244
            ++P       ++  G +LL     +E+ +  ++LPF++L G  DR+ D   +  L+E +
Sbjct: 202 NSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESS 261

Query: 245 SSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSR 292
            S DK+LK Y   +H +L+ E  E  + V  +I  W+  R  +  +R
Sbjct: 262 PSQDKTLKMYEGAYH-VLHKELPEVTNSVLHEINTWVSHRIAVAGAR 300

BLAST of CmoCh13G008850 vs. ExPASy TrEMBL
Match: A0A6J1ECU4 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111431373 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 5.6e-175
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 5   MMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEG 64
           MMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEG
Sbjct: 1   MMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEG 60

Query: 65  HGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPD 124
           HGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPD
Sbjct: 61  HGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPD 120

Query: 125 YWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQV 184
           YWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQV
Sbjct: 121 YWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQV 180

Query: 185 RNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKA 244
           RNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKA
Sbjct: 181 RNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKA 240

Query: 245 SSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQ 304
           SSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQ
Sbjct: 241 SSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQ 300

Query: 305 TK 307
           TK
Sbjct: 301 TK 302

BLAST of CmoCh13G008850 vs. ExPASy TrEMBL
Match: A0A6J1KJY0 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111495282 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 2.9e-171
Identity = 295/301 (98.01%), Postives = 297/301 (98.67%), Query Frame = 0

Query: 6   MNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGH 65
           MNS GLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNS+AIRLARAGFAVYGIDYEGH
Sbjct: 1   MNSCGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGH 60

Query: 66  GKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDY 125
           GKSDGLQGYITSFDHVVEDCSNHFTA SERKENMGKRRYLLGESMGGAVALLLHRKKPDY
Sbjct: 61  GKSDGLQGYITSFDHVVEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDY 120

Query: 126 WDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVR 185
           WDGAVLVAPMCKIAEDVKPKP+VVNIL KLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVR
Sbjct: 121 WDGAVLVAPMCKIAEDVKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVR 180

Query: 186 NNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKAS 245
           NNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGE DRVTDISVSKQLLEKAS
Sbjct: 181 NNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKAS 240

Query: 246 SWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQT 305
           SWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQT
Sbjct: 241 SWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQT 300

Query: 306 K 307
           K
Sbjct: 301 K 301

BLAST of CmoCh13G008850 vs. ExPASy TrEMBL
Match: A0A6J1GSV7 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111457223 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.8e-156
Identity = 262/303 (86.47%), Postives = 287/303 (94.72%), Query Frame = 0

Query: 4   FMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYE 63
           +++N+RGLKLFTC+W+PK++EPKGLIFICHGYAMECSITMNSTA+RLA+AGFAV+GIDYE
Sbjct: 15  YILNTRGLKLFTCKWIPKNQEPKGLIFICHGYAMECSITMNSTAVRLAKAGFAVHGIDYE 74

Query: 64  GHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKP 123
           GHGKSDGLQGYITSFDHVV+DCSNHFTA SE KEN  K RYLLGESMGGAVALLLHRKKP
Sbjct: 75  GHGKSDGLQGYITSFDHVVDDCSNHFTAISEMKENKRKMRYLLGESMGGAVALLLHRKKP 134

Query: 124 DYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQ 183
           DYWDGAVLVAPMCKIAEDVKP PIVV+IL KLC +IPTWKIIPTQDIIDVAFK PEIRHQ
Sbjct: 135 DYWDGAVLVAPMCKIAEDVKPSPIVVSILTKLCNLIPTWKIIPTQDIIDVAFKAPEIRHQ 194

Query: 184 VRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEK 243
           +R NPYC+KGRPRLNTG+ELL+ISLDLE+RLEEVS+PF+ILHGE DRVTDISVS+QL EK
Sbjct: 195 IRTNPYCYKGRPRLNTGHELLKISLDLEQRLEEVSIPFMILHGEADRVTDISVSEQLHEK 254

Query: 244 ASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDL 303
           ASSWDK LK YPEMWHGLLYGETVENID+VFADIIGWL+ERC LGNSRIE+QLKTE DDL
Sbjct: 255 ASSWDKRLKKYPEMWHGLLYGETVENIDIVFADIIGWLDERCALGNSRIEQQLKTECDDL 314

Query: 304 QTK 307
           QTK
Sbjct: 315 QTK 317

BLAST of CmoCh13G008850 vs. ExPASy TrEMBL
Match: A0A6J1DU62 (caffeoylshikimate esterase-like OS=Momordica charantia OX=3673 GN=LOC111024458 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.8e-155
Identity = 261/303 (86.14%), Postives = 284/303 (93.73%), Query Frame = 0

Query: 4   FMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYE 63
           +++NSRGLKLFTC+WLP ++EPKGLIFICHGYAMECSITM STAIRLA+AGFA+YG+DYE
Sbjct: 14  YVLNSRGLKLFTCQWLPTNQEPKGLIFICHGYAMECSITMKSTAIRLAKAGFAIYGVDYE 73

Query: 64  GHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKP 123
           GHGKSDGLQGYITSFDHVV+DCSNHFT+ SERK+N GK RYLLGESMGGAVALLLHRKKP
Sbjct: 74  GHGKSDGLQGYITSFDHVVDDCSNHFTSISERKQNKGKMRYLLGESMGGAVALLLHRKKP 133

Query: 124 DYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQ 183
           DYWDGAVLVAPMCKIA+DVKP PIVVNIL KLC  IPTWKIIPTQDIIDVAFKVPEIRHQ
Sbjct: 134 DYWDGAVLVAPMCKIADDVKPNPIVVNILTKLCNFIPTWKIIPTQDIIDVAFKVPEIRHQ 193

Query: 184 VRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEK 243
           +R NPYC+KGRPRLNTG++LL+IS DLE+RLEEVS PFIILHGEVDRVTDISVS+QL EK
Sbjct: 194 IRTNPYCYKGRPRLNTGHQLLKISSDLEQRLEEVSFPFIILHGEVDRVTDISVSEQLYEK 253

Query: 244 ASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDL 303
           ASSWDKSLKTYPEMWHGLLYGET+ENIDVVFA II WLEERC LGNSRIE+QLK+E DD 
Sbjct: 254 ASSWDKSLKTYPEMWHGLLYGETLENIDVVFAGIIAWLEERCALGNSRIERQLKSECDDQ 313

Query: 304 QTK 307
           Q K
Sbjct: 314 QAK 316

BLAST of CmoCh13G008850 vs. ExPASy TrEMBL
Match: A0A6J1JZ46 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111490153 PE=4 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.2e-154
Identity = 260/303 (85.81%), Postives = 286/303 (94.39%), Query Frame = 0

Query: 4   FMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYE 63
           +++N+RGLKLFTC+W+PK++EPKGLIFICHGYAMECSITMNSTA+RLA+AGFAV+GIDYE
Sbjct: 15  YILNTRGLKLFTCKWVPKNQEPKGLIFICHGYAMECSITMNSTAVRLAKAGFAVHGIDYE 74

Query: 64  GHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKP 123
           GHGKSDGLQGYITSFDHVV+DCSNHFTA SE KEN  K RYLLGESMGGA+ALLLHRKKP
Sbjct: 75  GHGKSDGLQGYITSFDHVVDDCSNHFTAISEMKENKMKMRYLLGESMGGAMALLLHRKKP 134

Query: 124 DYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQ 183
           DYWDGAVLVAPMCKIAE+VKP PIVV+IL KLC +IPTWKIIPTQDIIDVAFK PEIR Q
Sbjct: 135 DYWDGAVLVAPMCKIAENVKPSPIVVSILTKLCNLIPTWKIIPTQDIIDVAFKAPEIRRQ 194

Query: 184 VRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEK 243
           +R NPYC+KGRPRLNTG+ELLRISLDLE+RLEEVS+PF+ILHGE DRVTDISVS+QL  K
Sbjct: 195 IRTNPYCYKGRPRLNTGHELLRISLDLEQRLEEVSIPFMILHGEADRVTDISVSEQLHRK 254

Query: 244 ASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDL 303
           ASSWDKSLK YPEMWHGLLYGETVENID+VFADIIGWL+ERC LGNSRIE+QLKTE DDL
Sbjct: 255 ASSWDKSLKKYPEMWHGLLYGETVENIDIVFADIIGWLDERCALGNSRIEQQLKTECDDL 314

Query: 304 QTK 307
           QTK
Sbjct: 315 QTK 317

BLAST of CmoCh13G008850 vs. NCBI nr
Match: KAG7019788.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625.9 bits (1613), Expect = 1.8e-175
Identity = 302/305 (99.02%), Postives = 304/305 (99.67%), Query Frame = 0

Query: 2   QGFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID 61
           +GFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID
Sbjct: 13  EGFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID 72

Query: 62  YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK 121
           YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK
Sbjct: 73  YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK 132

Query: 122 KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIR 181
           KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIR
Sbjct: 133 KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIR 192

Query: 182 HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLL 241
           HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGE D+VTDISVSKQLL
Sbjct: 193 HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEADQVTDISVSKQLL 252

Query: 242 EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 301
           EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD
Sbjct: 253 EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 312

Query: 302 DLQTK 307
           DLQTK
Sbjct: 313 DLQTK 317

BLAST of CmoCh13G008850 vs. NCBI nr
Match: XP_022923765.1 (caffeoylshikimate esterase-like [Cucurbita moschata])

HSP 1 Score: 623.2 bits (1606), Expect = 1.2e-174
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 5   MMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEG 64
           MMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEG
Sbjct: 1   MMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEG 60

Query: 65  HGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPD 124
           HGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPD
Sbjct: 61  HGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPD 120

Query: 125 YWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQV 184
           YWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQV
Sbjct: 121 YWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQV 180

Query: 185 RNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKA 244
           RNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKA
Sbjct: 181 RNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKA 240

Query: 245 SSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQ 304
           SSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQ
Sbjct: 241 SSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQ 300

Query: 305 TK 307
           TK
Sbjct: 301 TK 302

BLAST of CmoCh13G008850 vs. NCBI nr
Match: XP_023520138.1 (caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.1 bits (1603), Expect = 2.6e-174
Identity = 300/305 (98.36%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 2   QGFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID 61
           +GF+MNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID
Sbjct: 16  EGFVMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID 75

Query: 62  YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK 121
           YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK
Sbjct: 76  YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK 135

Query: 122 KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIR 181
           KPDYWDGAVLVAPMCKIAEDVKPKPIVVNIL KLCKVIPTWKIIPTQDIIDVAFKVPEIR
Sbjct: 136 KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIR 195

Query: 182 HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLL 241
           HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGE DRVTDISVSKQLL
Sbjct: 196 HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLL 255

Query: 242 EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 301
           EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGN RIEKQLKTESD
Sbjct: 256 EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNLRIEKQLKTESD 315

Query: 302 DLQTK 307
           DLQTK
Sbjct: 316 DLQTK 320

BLAST of CmoCh13G008850 vs. NCBI nr
Match: XP_023001020.1 (caffeoylshikimate esterase-like [Cucurbita maxima])

HSP 1 Score: 610.9 bits (1574), Expect = 5.9e-171
Identity = 295/301 (98.01%), Postives = 297/301 (98.67%), Query Frame = 0

Query: 6   MNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGH 65
           MNS GLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNS+AIRLARAGFAVYGIDYEGH
Sbjct: 1   MNSCGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGH 60

Query: 66  GKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDY 125
           GKSDGLQGYITSFDHVVEDCSNHFTA SERKENMGKRRYLLGESMGGAVALLLHRKKPDY
Sbjct: 61  GKSDGLQGYITSFDHVVEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDY 120

Query: 126 WDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVR 185
           WDGAVLVAPMCKIAEDVKPKP+VVNIL KLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVR
Sbjct: 121 WDGAVLVAPMCKIAEDVKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVR 180

Query: 186 NNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKAS 245
           NNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGE DRVTDISVSKQLLEKAS
Sbjct: 181 NNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKAS 240

Query: 246 SWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQT 305
           SWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQT
Sbjct: 241 SWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQT 300

Query: 306 K 307
           K
Sbjct: 301 K 301

BLAST of CmoCh13G008850 vs. NCBI nr
Match: XP_038894800.1 (caffeoylshikimate esterase-like [Benincasa hispida])

HSP 1 Score: 565.8 bits (1457), Expect = 2.2e-157
Identity = 268/305 (87.87%), Postives = 287/305 (94.10%), Query Frame = 0

Query: 2   QGFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID 61
           + +++NSRG+KLFTC+WLPKDKEPKGLIFICHGYAMECSITMNSTAIRLA+AGFA+YGID
Sbjct: 13  EDYILNSRGVKLFTCKWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLAQAGFAIYGID 72

Query: 62  YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK 121
           YEGHGKSDGLQGYITSFD+VV+DCSN+FT  SERKEN GK RYLLGESMG AVALLLHRK
Sbjct: 73  YEGHGKSDGLQGYITSFDYVVDDCSNYFTEISERKENKGKMRYLLGESMGAAVALLLHRK 132

Query: 122 KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIR 181
           KPDYWDGAVLVAPMCKIAEDVKP PIVVNIL KLC VIPTWKIIPTQDIIDVAFKVPEIR
Sbjct: 133 KPDYWDGAVLVAPMCKIAEDVKPSPIVVNILTKLCNVIPTWKIIPTQDIIDVAFKVPEIR 192

Query: 182 HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLL 241
           HQ+R NPYC+KGRPRLNTG+ELLRISLDLE+RLEEVSLPFIILHGE DRVTD+S S+QL 
Sbjct: 193 HQIRTNPYCYKGRPRLNTGHELLRISLDLEQRLEEVSLPFIILHGEEDRVTDVSASEQLH 252

Query: 242 EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 301
           EKASSWDK+LK YPEMWHGLLYGET ENIDVVFADII WL+ERC LGNSRIEKQLKTE D
Sbjct: 253 EKASSWDKTLKKYPEMWHGLLYGETDENIDVVFADIIAWLDERCALGNSRIEKQLKTECD 312

Query: 302 DLQTK 307
           DLQTK
Sbjct: 313 DLQTK 317

BLAST of CmoCh13G008850 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 450.7 bits (1158), Expect = 9.6e-127
Identity = 210/305 (68.85%), Postives = 252/305 (82.62%), Query Frame = 0

Query: 2   QGFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID 61
           + F+ N+RG+KLFTC+W+P  +EPK L+FICHGYAMECSITMNSTA RL +AGFAVYGID
Sbjct: 12  ESFIKNTRGMKLFTCKWVPAKQEPKALVFICHGYAMECSITMNSTARRLVKAGFAVYGID 71

Query: 62  YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK 121
           YEGHGKSDGL  Y+ +FDH+V+D S H+T+  E++EN GK R+LLGESMGGAV LLLHRK
Sbjct: 72  YEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGAVLLLLHRK 131

Query: 122 KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIR 181
           KP +WDGAVLVAPMCKIAE++KP P+V++ILAKL  VIP+WKIIP QDII+ AFK PEIR
Sbjct: 132 KPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIETAFKQPEIR 191

Query: 182 HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLL 241
            QVR NPYC+KGRPRL T  ELLR+S DLEKRL EVSLPFI+LHGE D+VTD +VS+QL 
Sbjct: 192 KQVRENPYCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTDKAVSRQLY 251

Query: 242 EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 301
           E ASS DK+ K YP MWHGLLYGET ENI+ VFADIIGWL+++    +   E +LK ++D
Sbjct: 252 EVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWLDKKVADESGGFESELKRKND 311

Query: 302 DLQTK 307
            +  K
Sbjct: 312 GIPLK 316

BLAST of CmoCh13G008850 vs. TAIR 10
Match: AT2G39410.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 433.0 bits (1112), Expect = 2.1e-121
Identity = 200/305 (65.57%), Postives = 246/305 (80.66%), Query Frame = 0

Query: 2   QGFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID 61
           + F+ N+RG KLFTC WLP ++EP+ L+F+CHGY MECSITMNSTA RL +AGFAVYG+D
Sbjct: 12  ESFIKNTRGFKLFTCRWLPTNREPRALVFLCHGYGMECSITMNSTARRLVKAGFAVYGMD 71

Query: 62  YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK 121
           YEGHGKSDGL  YI++FD +V+D S H+TA  ER+EN  K R++LGESMGGAV LLL RK
Sbjct: 72  YEGHGKSDGLSAYISNFDRLVDDVSTHYTAICEREENKWKMRFMLGESMGGAVVLLLGRK 131

Query: 122 KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIR 181
            PD+WDGA+LVAPMCKIAE++KP P V++IL KL  +IP WKIIP+QDII++++K PEIR
Sbjct: 132 NPDFWDGAILVAPMCKIAEEMKPSPFVISILTKLISIIPKWKIIPSQDIIEISYKEPEIR 191

Query: 182 HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLL 241
            QVR NP C KGRPRL T  ELLRIS DLEKRL+EVSLPF++LHG+ D+VTD +VS++L 
Sbjct: 192 KQVRENPLCSKGRPRLKTAYELLRISNDLEKRLQEVSLPFLVLHGDDDKVTDKAVSQELY 251

Query: 242 EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 301
           + A S DK+LK YP MWHGLL GET ENI++VFAD+I WLE+R   GN R E +LK  +D
Sbjct: 252 KVALSADKTLKLYPGMWHGLLTGETPENIEIVFADVISWLEKRSDYGNDRFESELKQRND 311

Query: 302 DLQTK 307
            L  K
Sbjct: 312 RLNFK 316

BLAST of CmoCh13G008850 vs. TAIR 10
Match: AT2G39400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 1.3e-120
Identity = 199/300 (66.33%), Postives = 242/300 (80.67%), Query Frame = 0

Query: 2   QGFMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGID 61
           + F++NSRG+KLFTC W P  +EPK L+F+CHGYAME SITMNS A RLA AGFAVYG+D
Sbjct: 6   EDFVLNSRGMKLFTCVWKPVKQEPKALLFLCHGYAMESSITMNSAATRLANAGFAVYGMD 65

Query: 62  YEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRK 121
           YEGHGKS+GL GYI++FD +V+D SNH++   ER+EN GK R+LLGESMGGAV LLL RK
Sbjct: 66  YEGHGKSEGLNGYISNFDDLVDDVSNHYSTICEREENKGKMRFLLGESMGGAVVLLLARK 125

Query: 122 KPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIR 181
           KPD+WDGAVLVAPMCK+A+++KP P+V++IL KL K IPTWKI+P  DIID+A K P IR
Sbjct: 126 KPDFWDGAVLVAPMCKLADEIKPHPVVISILIKLAKFIPTWKIVPGNDIIDIAIKEPHIR 185

Query: 182 HQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLL 241
           +QVR N YC+KGRPRLNT  +LL +SLDLEK L +VS+PFI+LHGE D+VTD S+SK L 
Sbjct: 186 NQVRENKYCYKGRPRLNTAYQLLLVSLDLEKNLHQVSIPFIVLHGEDDKVTDKSISKMLY 245

Query: 242 EKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 301
           E ASS DK+ K YP+MWH LLYGET EN ++VF DII WLE+R    N  +E QLK + D
Sbjct: 246 EVASSSDKTFKLYPKMWHALLYGETNENSEIVFGDIINWLEDRATDSNGGLESQLKHKHD 305

BLAST of CmoCh13G008850 vs. TAIR 10
Match: AT3G55180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 415.6 bits (1067), Expect = 3.4e-116
Identity = 190/301 (63.12%), Postives = 245/301 (81.40%), Query Frame = 0

Query: 2   QGFMMNSRGLKLFTCEWLPKD-KEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGI 61
           + ++ NSRG++LFTC W  ++ +EPK LIF+CHGYAME SITM+STA+RLA AGF+VYG+
Sbjct: 6   EDYVSNSRGIQLFTCSWKQEEQQEPKALIFLCHGYAMESSITMSSTAVRLANAGFSVYGM 65

Query: 62  DYEGHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHR 121
           DYEGHGKS GL GY+  FD +V+D S+H+++  E +EN GK R+L+GESMGGAV LLL R
Sbjct: 66  DYEGHGKSGGLNGYVKKFDDLVQDVSSHYSSICELEENKGKMRFLMGESMGGAVVLLLER 125

Query: 122 KKPDYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEI 181
           KKP++WDGAVLVAPMCK+AED+KP P+V++ L KL + IPTWKI+P+ DIIDVAFK   I
Sbjct: 126 KKPNFWDGAVLVAPMCKLAEDIKPHPMVISFLTKLTRFIPTWKIVPSNDIIDVAFKETHI 185

Query: 182 RHQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQL 241
           R QVR+N YC+KGRPRL T ++LL +SLDLEK L++VS+PFI+LHGE D+VTD +VSK L
Sbjct: 186 RKQVRDNEYCYKGRPRLKTAHQLLMVSLDLEKNLDQVSMPFIVLHGEDDKVTDKNVSKLL 245

Query: 242 LEKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTES 301
            E ASS DK+ K YP MWHGLLYGE+ EN+++VF+DII WL+ER  + N ++E +LK   
Sbjct: 246 YEVASSSDKTFKLYPNMWHGLLYGESPENLEIVFSDIISWLKERASVTNQKLETELKHVD 305

BLAST of CmoCh13G008850 vs. TAIR 10
Match: AT3G55190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 347.4 bits (890), Expect = 1.1e-95
Identity = 158/282 (56.03%), Postives = 215/282 (76.24%), Query Frame = 0

Query: 4   FMMNSRGLKLFTCEWLPKDKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYE 63
           F+ NSRG++L TC+W P ++EP+ LIF CHGYA++CS T    A + A+ GFAV+GI+YE
Sbjct: 14  FIENSRGMQLLTCKWFPVNQEPRALIFFCHGYAIDCSTTFKDIAPKFAKEGFAVHGIEYE 73

Query: 64  GHGKSDGLQGYITSFDHVVEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKP 123
           GHG+S GL  YI +FD +++D S+HF+  SE  +N  K+R+L+GESMGGAV LLLHRKKP
Sbjct: 74  GHGRSSGLSVYIDNFDLLIDDVSSHFSKISEMGDNTKKKRFLMGESMGGAVVLLLHRKKP 133

Query: 124 DYWDGAVLVAPMCKIAEDVKPKPIVVNILAKLCKVIPTWK-IIPTQDIIDVAFKVPEIRH 183
           ++WDG +L+APMCKIAE++KP  +V++++  +  +IP+WK II   DI++ A K+PE RH
Sbjct: 134 EFWDGGILIAPMCKIAEEMKPSRMVISMINMVTNLIPSWKSIIHGPDILNSAIKLPEKRH 193

Query: 184 QVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLE 243
           ++R NP C+ G PR+ T +EL RISLDLE RL EV++PFI+LHGE D+VTD   SK L E
Sbjct: 194 EIRTNPNCYNGWPRMKTMSELFRISLDLENRLNEVTMPFIVLHGEDDKVTDKGGSKLLYE 253

Query: 244 KASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEER 285
            A S DK+LK YPEMWH LL+GE  EN ++VF DI+ W++ R
Sbjct: 254 VALSNDKTLKLYPEMWHSLLFGEPPENSEIVFNDIVQWMQTR 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9421.4e-4235.04Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1[more]
O074271.6e-2530.22Monoacylglycerol lipase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv... [more]
Q996858.7e-2430.36Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2[more]
A0QNZ74.3e-2325.64Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(... [more]
Q8R4315.6e-2327.18Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ECU45.6e-175100.00caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111431373 PE... [more]
A0A6J1KJY02.9e-17198.01caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111495282 PE=4... [more]
A0A6J1GSV75.8e-15686.47caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111457223 PE... [more]
A0A6J1DU623.8e-15586.14caffeoylshikimate esterase-like OS=Momordica charantia OX=3673 GN=LOC111024458 P... [more]
A0A6J1JZ463.2e-15485.81caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111490153 PE=4... [more]
Match NameE-valueIdentityDescription
KAG7019788.11.8e-17599.02Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022923765.11.2e-174100.00caffeoylshikimate esterase-like [Cucurbita moschata][more]
XP_023520138.12.6e-17498.36caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo][more]
XP_023001020.15.9e-17198.01caffeoylshikimate esterase-like [Cucurbita maxima][more]
XP_038894800.12.2e-15787.87caffeoylshikimate esterase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G39420.19.6e-12768.85alpha/beta-Hydrolases superfamily protein [more]
AT2G39410.22.1e-12165.57alpha/beta-Hydrolases superfamily protein [more]
AT2G39400.11.3e-12066.33alpha/beta-Hydrolases superfamily protein [more]
AT3G55180.13.4e-11663.12alpha/beta-Hydrolases superfamily protein [more]
AT3G55190.11.1e-9556.03alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 54..69
score: 36.85
coord: 105..118
score: 50.74
coord: 221..235
score: 28.74
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..286
e-value: 5.8E-56
score: 191.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 5..282
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 24..262
e-value: 1.1E-61
score: 208.2
NoneNo IPR availablePANTHERPTHR11614PHOSPHOLIPASE-RELATEDcoord: 3..300
NoneNo IPR availablePANTHERPTHR11614:SF176ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 3..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G008850.1CmoCh13G008850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016298 lipase activity