CmoCh13G006760 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G006760
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionpolyphenol oxidase, chloroplastic-like
LocationCmo_Chr13: 7034014 .. 7036234 (+)
RNA-Seq ExpressionCmoCh13G006760
SyntenyCmoCh13G006760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCGCTATCACCACCAGCTCTTTCCACCACCACATTTCGCCCATCATCCAGCCTTAGAACATCAAAATACCTTCCCAATAACGCTGCTCGAATTTTGTGTAGCGCCGCCGATGGCAATATTGATAGAAGAGACGTTCTTATTGGCATAGGCGCAGGCGGTCTCTACGGAGCTTCCGCCTTTGCCCTTGGCGCTCCAGTCCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACCTGCCACCTGACGCTGACCCAACGAACTGCTGCCCGCCGCCGTCCCCCTCGATCGTCGACTTCCGACCACCGGCACCGGGAAAATTACGGGTCAGGCCAGCTGCCCATTTGGTCGATCAAAGCTACATAGACAAATATAAGAGGGCCGTGGAGCTAATGAAAGGGCTGCCCGAGGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTGATTGGAGATCCCACTCTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAGAATGCCAGCCATGTACGCCGACCACAACTCCTCGCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACGTTGATTGACCTGGATTTCGATGGGGTTGATCCGACCATAGGCGATGATGCGCTGATAAAAAGTAATCTTAGAATTATGTATCGCCAGATGGTTTCCAATAGTGTCACGCCCAAGCTGTTCTTCGGCAGCGCTTATGTAGCCGGAGACGAGCCATCACCGGGTAACGCTTTTCTTTTTTCCAACTTTTTCCCAACTCTCTATGATATTTTACACTTTGAGCATAAGCTCTCATGCAACAATGGAGAGAGTATTCTTTGTTTATAAAATCATGATTATTCCCTAAATTAGTCGACGTGGGACTTTCATCATCCAACACCTTCCCTCGAACAAAGGCTCGACTCCTTTGGAGTCTTAGTAATTTTTGGCCTTCGAGGAGACTTAACTCCTTTTCTTTTGGAGTCCTTTGTTCGATATTTGAGAATTTACTAATCTATTGGCACGACTAAGTTTAGGGCATGACTCTGATACCATGTTAGACGAACATGACTCTCCACAATGGTATGATATTGTCTCAGTATAAACTCTCATGGATTTACTCTAGGTTTCCCAAAATGTGGTATGATATTGTCTCAGTATAAACTCTCATGGATTTACTCTAGGTTTCCCAAAATGGCATACACGAATGGACAAAGTATTGTTTATTTAATTTCTATTCTGAGTACGATGGAGGTTAGTTTTGTGGTGGGATAGAAGGGTAGTTAATTAACTTAGTATACACAAAATGACAGGAGGTGGGTCAGTGGAGAACATCCCACACAATACGGTCCACACATGGTGCGGCGACAACAAGAAGCCCAACCTAGAGAACATGGGCAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACGTAGACCGCATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACAGGACCTTCAAAACCCTGAATGGCTCAACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAAACTCGGCTACGTCTACCAAGACGTGGAACTCCCATGGCTCCAAACCCGCCCCATGCCTCGTGGAGCCCGGAAGGCGGCGAGACATCCAAAGATGAAGTTTCCGGTAACTCTGAAGTCGGCGATCAGTACGGAAGTGAAGAGGGCGAAGAAATCAAGAAGCAAGAAGGAGAAAGAGGAGGAAGAGGAGATTTTGGTGATTGAGGGAATCAAGTTGGACCCCAACATGCTGGTAAAGTTCGATATTTATATCAACGACGAAGATGAGAAGGAAAATAGAGCCGACATTACTGAGTTCGCTGGGAGCTTTGTGAACGTGCCGCATAAGCACGGACACAACAAGAAGGTGAAGACGGGGCTTAGGTTGGGGATAACTGAACTGCTTGAGGATTTGGGTGCTGATGGTGACGACAGCATCATCGTCACTCTCGTCCCCAGGCTTGGAGCTGGGCTCGTCTCCATTGCTAAGATCAAGATCGAATATGGAGATTAA

mRNA sequence

ATGGCTTCGCTATCACCACCAGCTCTTTCCACCACCACATTTCGCCCATCATCCAGCCTTAGAACATCAAAATACCTTCCCAATAACGCTGCTCGAATTTTGTGTAGCGCCGCCGATGGCAATATTGATAGAAGAGACGTTCTTATTGGCATAGGCGCAGGCGGTCTCTACGGAGCTTCCGCCTTTGCCCTTGGCGCTCCAGTCCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACCTGCCACCTGACGCTGACCCAACGAACTGCTGCCCGCCGCCGTCCCCCTCGATCGTCGACTTCCGACCACCGGCACCGGGAAAATTACGGGTCAGGCCAGCTGCCCATTTGGTCGATCAAAGCTACATAGACAAATATAAGAGGGCCGTGGAGCTAATGAAAGGGCTGCCCGAGGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTGATTGGAGATCCCACTCTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAGAATGCCAGCCATGTACGCCGACCACAACTCCTCGCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACGTTGATTGACCTGGATTTCGATGGGGTTGATCCGACCATAGGCGATGATGCGCTGATAAAAAGTAATCTTAGAATTATGTATCGCCAGATGGTTTCCAATAGTGTCACGCCCAAGCTGTTCTTCGGCAGCGCTTATGTAGCCGGAGACGAGCCATCACCGGGAGGTGGGTCAGTGGAGAACATCCCACACAATACGGTCCACACATGGTGCGGCGACAACAAGAAGCCCAACCTAGAGAACATGGGCAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACGTAGACCGCATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACAGGACCTTCAAAACCCTGAATGGCTCAACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAAACTCGGCTACGTCTACCAAGACGTGGAACTCCCATGGCTCCAAACCCGCCCCATGCCTCGTGGAGCCCGGAAGGCGGCGAGACATCCAAAGATGAAGTTTCCGGTAACTCTGAAGTCGGCGATCAGTACGGAAGTGAAGAGGGCGAAGAAATCAAGAAGCAAGAAGGAGAAAGAGGAGGAAGAGGAGATTTTGGTGATTGAGGGAATCAAGTTGGACCCCAACATGCTGGTAAAGTTCGATATTTATATCAACGACGAAGATGAGAAGGAAAATAGAGCCGACATTACTGAGTTCGCTGGGAGCTTTGTGAACGTGCCGCATAAGCACGGACACAACAAGAAGGTGAAGACGGGGCTTAGGTTGGGGATAACTGAACTGCTTGAGGATTTGGGTGCTGATGGTGACGACAGCATCATCGTCACTCTCGTCCCCAGGCTTGGAGCTGGGCTCGTCTCCATTGCTAAGATCAAGATCGAATATGGAGATTAA

Coding sequence (CDS)

ATGGCTTCGCTATCACCACCAGCTCTTTCCACCACCACATTTCGCCCATCATCCAGCCTTAGAACATCAAAATACCTTCCCAATAACGCTGCTCGAATTTTGTGTAGCGCCGCCGATGGCAATATTGATAGAAGAGACGTTCTTATTGGCATAGGCGCAGGCGGTCTCTACGGAGCTTCCGCCTTTGCCCTTGGCGCTCCAGTCCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACCTGCCACCTGACGCTGACCCAACGAACTGCTGCCCGCCGCCGTCCCCCTCGATCGTCGACTTCCGACCACCGGCACCGGGAAAATTACGGGTCAGGCCAGCTGCCCATTTGGTCGATCAAAGCTACATAGACAAATATAAGAGGGCCGTGGAGCTAATGAAAGGGCTGCCCGAGGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTGATTGGAGATCCCACTCTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAGAATGCCAGCCATGTACGCCGACCACAACTCCTCGCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACGTTGATTGACCTGGATTTCGATGGGGTTGATCCGACCATAGGCGATGATGCGCTGATAAAAAGTAATCTTAGAATTATGTATCGCCAGATGGTTTCCAATAGTGTCACGCCCAAGCTGTTCTTCGGCAGCGCTTATGTAGCCGGAGACGAGCCATCACCGGGAGGTGGGTCAGTGGAGAACATCCCACACAATACGGTCCACACATGGTGCGGCGACAACAAGAAGCCCAACCTAGAGAACATGGGCAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACGTAGACCGCATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACAGGACCTTCAAAACCCTGAATGGCTCAACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAAACTCGGCTACGTCTACCAAGACGTGGAACTCCCATGGCTCCAAACCCGCCCCATGCCTCGTGGAGCCCGGAAGGCGGCGAGACATCCAAAGATGAAGTTTCCGGTAACTCTGAAGTCGGCGATCAGTACGGAAGTGAAGAGGGCGAAGAAATCAAGAAGCAAGAAGGAGAAAGAGGAGGAAGAGGAGATTTTGGTGATTGAGGGAATCAAGTTGGACCCCAACATGCTGGTAAAGTTCGATATTTATATCAACGACGAAGATGAGAAGGAAAATAGAGCCGACATTACTGAGTTCGCTGGGAGCTTTGTGAACGTGCCGCATAAGCACGGACACAACAAGAAGGTGAAGACGGGGCTTAGGTTGGGGATAACTGAACTGCTTGAGGATTTGGGTGCTGATGGTGACGACAGCATCATCGTCACTCTCGTCCCCAGGCTTGGAGCTGGGCTCGTCTCCATTGCTAAGATCAAGATCGAATATGGAGATTAA

Protein sequence

MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGASAFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVSIAKIKIEYGD
Homology
BLAST of CmoCh13G006760 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 4.1e-197
Identity = 343/595 (57.65%), Postives = 427/595 (71.76%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNN-------------AARILCSA--------AD 60
           M SLSPP ++T T    ++   S +  NN             A ++ C A        A 
Sbjct: 1   MTSLSPPVVTTPTVPNPATKPLSPFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQ 60

Query: 61  GNIDRRDVLIGIGAGGLYGASA-----FALGAPVQTPNISKCGPPDLPPDADPTNCCPPP 120
             +DRR+VL+G+  GGLYG +      FA   P+  P++SKCGP DLP  A PTNCCPPP
Sbjct: 61  SKLDRRNVLLGL--GGLYGVAGMGTDPFAFAKPIAPPDVSKCGPADLPQGAVPTNCCPPP 120

Query: 121 SPSIVDFRPPAPGKLRVRPAAHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAY 180
           S  I+DF+ PAP KLR+RP AH VDQ+Y DKY +A+ELMK LP+DDPRSF QQA VHCAY
Sbjct: 121 STKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAY 180

Query: 181 CNGGYDQVGFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGM 240
           C+G YDQVGFP +E+Q+HNSWLFFPFHRYYLYF+EKILG+LI DPT ALPFWN+D+P GM
Sbjct: 181 CDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGM 240

Query: 241 RMPAMYADHNSSLYDQLRNQNHLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNS 300
            +PA+YAD  S LYD+LR+ NH PPTL+DLD++G +  +  +  I +NL+IMYRQMVSNS
Sbjct: 241 PLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNS 300

Query: 301 VTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFY 360
              KLFFG+ Y AGDEP PGGGS+E  PH  VH W GDN +PN E+MGNFYSA RDPIF+
Sbjct: 301 KNAKLFFGNPYRAGDEPDPGGGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIFF 360

Query: 361 SHHSNVDRMWSVWKTLGGRRQDLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVY 420
           +HHSNVDRMWS+WKTLGG+R DL + +WL++ F+FYNE A+ VRV+VRDCL+TK LGYVY
Sbjct: 361 AHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVY 420

Query: 421 QDVELPWLQTRPMPRGARKAARHPKMK-------------------FPVTLKSAISTEVK 480
           QDV++PWL ++P PR A+ A      K                   FP++L S IST VK
Sbjct: 421 QDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVVK 480

Query: 481 RAK-KSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENRADITEFAGSFVNV 540
           R K K RSKK KE+EEEILVIEGI+ D ++ VKFD+Y+ND D+  +  D TEFAGSFV+V
Sbjct: 481 RPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDKTEFAGSFVSV 540

Query: 541 PHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVSIAKIKIEY 549
           PH H H KK+ T LRLG+T+LLE++ A+ DDS++VTLVP+ GA  V I  IKIE+
Sbjct: 541 PHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGA--VKIGGIKIEF 591

BLAST of CmoCh13G006760 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 8.2e-174
Identity = 305/535 (57.01%), Postives = 377/535 (70.47%), Query Frame = 0

Query: 38  ADGNIDRRDVLIGIG-----AGGLYGASAFALGAPVQTPNISKCGPPDLPPDADPTNCCP 97
           + G +DRR+VL+GIG     AGGL      A GAP+Q P+ISKCG   +P    PTNCCP
Sbjct: 71  SSGKLDRRNVLLGIGGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCP 130

Query: 98  PPSPSIVDFRPPAPGK-LRVRPAAHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVH 157
           P +  I+DF+ P+ G  +R RPAAHLV + Y+ KYK+A+EL K LP+DDPRSF QQANVH
Sbjct: 131 PVTTKIIDFQLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVH 190

Query: 158 CAYCNGGYDQVGF-PVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAP 217
           C YC G YDQVG+  +E+QVH SWLF PFHRYYLYF E+IL +LI DPT ALP+W +D P
Sbjct: 191 CTYCQGAYDQVGYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNP 250

Query: 218 GGMRMPAMYADHNSSLYDQLRNQNHLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMV 277
            GM MP +YA   SSLYD+ RN  HLPPT+IDLD+DG +PTI DD L   NL IMY+Q+V
Sbjct: 251 DGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIV 310

Query: 278 SNSVTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDP 337
           S + TPKLF G  Y AGD   PG G++E+ PHN VH W G   KP+ E+MGNFY+A RDP
Sbjct: 311 SGATTPKLFLGYPYRAGDAIDPGAGTLEHAPHNIVHKWTGLADKPS-EDMGNFYTAGRDP 370

Query: 338 IFYSHHSNVDRMWSVWKTLGGR-RQDLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKL 397
           IF+ HH+NVDRMW++WKT+GG+ R+D  + +WL+A+F+FY+E  Q V+V+V DC+DT KL
Sbjct: 371 IFFGHHANVDRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKL 430

Query: 398 GYVYQDVELPWL----------QTRPMPRGARKAARHPKM------KFPVTLKSAISTEV 457
            Y YQD+ +PWL           T+    G  KAA  PK        FP  L S I  EV
Sbjct: 431 RYQYQDIPIPWLPKNTKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEV 490

Query: 458 KRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENRADITEFAGSFVNV 517
            R KKSRSKKEKE+EEE+L+I+GI+LD    VKFD+YINDED   +R   +EFAGSFVNV
Sbjct: 491 PRPKKSRSKKEKEDEEEVLLIKGIELDRENFVKFDVYINDEDYSVSRPKNSEFAGSFVNV 550

Query: 518 PHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVSIAKIKIEY 549
           PHKH    K KT LR  I ELLEDLGA+ D+S+IVT+VPR G   V+I  I+IE+
Sbjct: 551 PHKHMKEMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIEF 604

BLAST of CmoCh13G006760 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 7.0e-173
Identity = 306/540 (56.67%), Postives = 383/540 (70.93%), Query Frame = 0

Query: 44  RRDVLIGIGAGGLYGASA---FALGAPVQTPNISKCGPP-DLPPDADP--TNCCPPPSPS 103
           RR+VLIG+  GG+YG  A    AL +P+  P++SKC PP DLP    P   NCCPP S  
Sbjct: 70  RRNVLIGL--GGIYGTLATNPSALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPYSTK 129

Query: 104 IVDFRPPAPGKLRVRPAAHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNG 163
           I DF+ P+   LRVR AAHLVD  +++KYK+A ELMK LP +DPR+F QQAN+HCAYC+G
Sbjct: 130 ITDFKFPSNQPLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDG 189

Query: 164 GYDQVGFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMP 223
            Y Q+GFP +++QVH SWLFFPFHR+YLYFYE+ILG LI DPT ALPFWNYDAP GM++P
Sbjct: 190 AYSQIGFPDLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLP 249

Query: 224 AMYADHNSSLYDQLRNQNHLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTP 283
            +YAD  S LYD+LRN +H PPTLIDL+F  +   I  + LIK+NL IMYRQ+ SN  T 
Sbjct: 250 TIYADKASPLYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTS 309

Query: 284 KLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHH 343
           +LF G+ Y AGD    G GS+EN+PH  VHTW GDN + N+E+MG FYSAARDPIFYSHH
Sbjct: 310 RLFLGNPYRAGDAEPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHH 369

Query: 344 SNVDRMWSVWKTLGGRRQDLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDV 403
           SNVDR+W +WKTLGG++ D  + +WL + F+FY+E    VRV V+D LD  KLGY YQDV
Sbjct: 370 SNVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDV 429

Query: 404 ELPWLQTRPMPRG------------------------ARKAARHPKMKFPVTLKSAISTE 463
            +PW + +P+PR                          R+ +    + FP+ L + +S  
Sbjct: 430 PIPWEKAKPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAI 489

Query: 464 VKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENRADITEFAGSFVN 523
           VKR KK RSKKEKEEEEE+LVIEGI+   N+ +KFD+YINDED+K   A  TEFAGSFVN
Sbjct: 490 VKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYINDEDDKVG-AGNTEFAGSFVN 549

Query: 524 VPHK-HGH-NKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVSIAKIKIEYGD 551
           +PH  HGH NKK+ T LRLGIT+LLEDL  +GDD+I+VTLVP+ G+G V I  ++I + D
Sbjct: 550 IPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEIVFED 606

BLAST of CmoCh13G006760 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 572.4 bits (1474), Expect = 5.5e-162
Identity = 292/572 (51.05%), Postives = 392/572 (68.53%), Query Frame = 0

Query: 3   SLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGN----IDRRDVLIGIGAGGLYG 62
           SLS  A  ++     S +  +     +  ++ C+A +G+    +DRRDVL+G+  GGL G
Sbjct: 19  SLSSSATISSPLPKPSQIHIATAKRTHHFKVSCNAPNGDSQPKLDRRDVLLGL--GGLAG 78

Query: 63  ASA-----FALGAPVQTPNISKC--GPPDLPPDADPTNCCPPPSPSIVDFRPP--APGKL 122
           A++      A   P+  P ISKC   P DLPPDA   NCCPP + +++ ++ P  +P  +
Sbjct: 79  AASLINNPLAFAEPIHAPEISKCVVPPKDLPPDAIVDNCCPPLATNVIPYKVPKTSPSAM 138

Query: 123 RVRPAAHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFP-VEV 182
           ++RPA H +D+ YI K+++A+ LMK LP DDPR+F QQA VHCAYCNGGY Q  +P  E+
Sbjct: 139 KIRPAIHRMDKEYIAKFEKAIRLMKELPADDPRNFYQQALVHCAYCNGGYVQTDYPDKEI 198

Query: 183 QVHNSWLFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYD 242
           QVHNSWLFFPFHR+YLYFYE+ILG+LIGDPT  LPFWN+D P GM +P  + + NS LYD
Sbjct: 199 QVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGMLIPQYFRNQNSPLYD 258

Query: 243 QLRNQNHLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGD 302
           + RNQ+HL P ++DL + G D  + D   I +NL +MY+ MV+N+ T +LF G  Y AGD
Sbjct: 259 ENRNQSHL-PLVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAGTAELFLGKPYKAGD 318

Query: 303 EP-SPGGGSVENIPHNTVHTWCGDNK--KPNLENMGNFYSAARDPIFYSHHSNVDRMWSV 362
           +P + GGGS+ENIPH  VH W GD K    N E+MGNFYSA RD +FY HHSNVDRMW++
Sbjct: 319 DPVNKGGGSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDILFYCHHSNVDRMWTI 378

Query: 363 WKTLG--GRRQDLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQT 422
           W+ LG  GRR+D  + +WL+A+FIFY+E  QAVRVRV D LD +KLGY Y+   LPWL +
Sbjct: 379 WQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKLGYKYEFTNLPWLNS 438

Query: 423 RPMPR------GARKAARHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGI 482
           +P+P        AR  A      FP+TL   +  +V R KKSRSK+EKE EEEIL I+GI
Sbjct: 439 KPLPTKKKTGLAARSKAPFVTDVFPLTLDKVVQVKVPRPKKSRSKEEKEAEEEILEIQGI 498

Query: 483 KLDPNMLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLED 542
           ++  +   KFD+Y+NDEDE E   +  E+AGSF ++PHKH  +KK++T L LG+ E LED
Sbjct: 499 EVAIDQYAKFDVYLNDEDEPEAGKEKAEYAGSFAHLPHKHTGSKKIRTSLSLGLNEPLED 558

Query: 543 LGADGDDSIIVTLVPRLGAGLVSIAKIKIEYG 550
           LGA+ DD+++VTL P++G G+V++  IKI YG
Sbjct: 559 LGAEDDDAVLVTLAPKVGGGVVTVENIKIVYG 587

BLAST of CmoCh13G006760 vs. ExPASy Swiss-Prot
Match: Q9ZP19 (Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-1 PE=1 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.6e-161
Identity = 273/495 (55.15%), Postives = 352/495 (71.11%), Query Frame = 0

Query: 66  APVQTPNISKC--GPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQSYI 125
           AP+Q P ISKC   P DLPP A   NCCPP + +IVD++ PA   ++VRPAAH +D+  I
Sbjct: 1   APIQAPEISKCVVPPADLPPGAVVDNCCPPVASNIVDYKLPAVTTMKVRPAAHTMDKDAI 60

Query: 126 DKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFP-VEVQVHNSWLFFPFHRY 185
            K+ +AVELMK LP DDPR+F QQA VHCAYCNGGYDQV FP  E+QVHNSWLFFPFHR+
Sbjct: 61  AKFAKAVELMKALPADDPRNFYQQALVHCAYCNGGYDQVNFPDQEIQVHNSWLFFPFHRW 120

Query: 186 YLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTLID 245
           YLYFYE+ILG+LIGDP+  LPFWN+D PGGM +P    D  SSLYD  RNQ+HLPP ++D
Sbjct: 121 YLYFYERILGKLIGDPSFGLPFWNWDNPGGMVLPDFLNDSTSSLYDSNRNQSHLPPVVVD 180

Query: 246 LDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENIPH 305
           L ++G D  + D   I  NL +MY+QMV+N+ T +LF G AY AGD PSPG GS+E  PH
Sbjct: 181 LGYNGADTDVTDQQRITDNLALMYKQMVTNAGTAELFLGKAYRAGDAPSPGAGSIETSPH 240

Query: 306 NTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGG--RRQDLQNPE 365
             +H W GD +  N E+MGNFYSA RD  FY HHSNVDRMW++W+ L G  R++D  + +
Sbjct: 241 IPIHRWVGDPRNTNNEDMGNFYSAGRDIAFYCHHSNVDRMWTIWQQLAGKPRKRDYTDSD 300

Query: 366 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPR------GARKAA 425
           WLNA+F+FY+E  QAV+VR+ D LD +K+GY Y    LPWL ++P+P        ++  A
Sbjct: 301 WLNATFLFYDENGQAVKVRIGDSLDNQKMGYKYAKTPLPWLDSKPVPTKKKGGYASKSKA 360

Query: 426 RHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDE 485
                 FPVTL   +  +V R KKSRS +EKE EEEIL+I GI+++ +   KFD+Y+ND 
Sbjct: 361 PFVASVFPVTLDKVVQVKVARPKKSRSAEEKEAEEEILLIVGIEVEIDKYAKFDVYLNDS 420

Query: 486 DEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRL 545
           D+     D  E+AGSF ++PHKH   KK++T L LG+ E LEDLGA+ DD+I+VTL P++
Sbjct: 421 DDPSGGKDKAEYAGSFAHLPHKHKGMKKIRTTLSLGLNEPLEDLGAEDDDTILVTLAPKV 480

Query: 546 GAGLVSIAKIKIEYG 550
           G G+VS+  +K+ YG
Sbjct: 481 GGGVVSVDNVKVVYG 495

BLAST of CmoCh13G006760 vs. ExPASy TrEMBL
Match: A0A6J1EMV7 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434014 PE=3 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ 120
           AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ
Sbjct: 61  AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ 120

Query: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180
           SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH
Sbjct: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180

Query: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240
           RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL
Sbjct: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240

Query: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI 300
           IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI
Sbjct: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI 300

Query: 301 PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE 360
           PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE
Sbjct: 301 PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE 360

Query: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420
           WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK
Sbjct: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420

Query: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR 480
           FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR
Sbjct: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR 480

Query: 481 ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS 540
           ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS
Sbjct: 481 ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS 540

Query: 541 IAKIKIEYGD 551
           IAKIKIEYGD
Sbjct: 541 IAKIKIEYGD 550

BLAST of CmoCh13G006760 vs. ExPASy TrEMBL
Match: A0A6J1KJD3 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495764 PE=3 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.6e-297
Identity = 508/568 (89.44%), Postives = 523/568 (92.08%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           M SLS PALSTTTFRPSS   TSKYLPNNAAR+LCSAADG IDRRDVLIGIGAGG+YGAS
Sbjct: 1   MGSLSSPALSTTTFRPSSRPPTSKYLPNNAARVLCSAADGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  A------FALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           A      FAL APVQTPNISKC PPDLP  ADPTNCCPPPSPSIVDFRPP PGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCSPPDLPHGADPTNCCPPPSPSIVDFRPPVPGKLRVRPA 120

Query: 121 AHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHL DQSYIDKYKRAVELMKGLP DDPRSFMQQAN+HCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANIHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPT ALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300
           HLPPTL+DLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDN +PNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKA- 420
           DLQ PEWL+ASFIFYNEKAQAVRVRVRDCLDTK LGY+YQDVELPWL+TRP P  A +A 
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPCRALRAQ 420

Query: 421 -----------ARHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPN 480
                       + PKMKFPVTLKSAISTEVKR KKSRSKKEKEEEEEILVIEGIKLDPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKFPVTLKSAISTEVKRGKKSRSKKEKEEEEEILVIEGIKLDPN 480

Query: 481 MLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADG 540
           MLVKFDIYINDEDEKENRADI+EFAGSFVNVPHKHGH KK+KTGLRLGIT+LLEDL AD 
Sbjct: 481 MLVKFDIYINDEDEKENRADISEFAGSFVNVPHKHGHKKKMKTGLRLGITQLLEDLDADC 540

Query: 541 DDSIIVTLVPRLGAGLVSIAKIKIEYGD 551
           DDSIIVTL+PRLGAGLVSIAKIKIEYGD
Sbjct: 541 DDSIIVTLIPRLGAGLVSIAKIKIEYGD 568

BLAST of CmoCh13G006760 vs. ExPASy TrEMBL
Match: A0A6J1KRC0 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495765 PE=3 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.5e-287
Identity = 488/568 (85.92%), Postives = 514/568 (90.49%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           MASL PPALST TFRPSS   TSKYLPNNAAR+LCSAA+G IDRRDVLIGIGAGG+YGAS
Sbjct: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  A------FALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           A      FAL APVQTPNISKCGPPDLPP +DPTNCCPPPSPSIVDFRPPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120

Query: 121 AHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHL DQSYIDKYKRAVELMKGLP DDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPT ALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300
           HLPPTL+DLDFDGVD  IGD+A I+SNLRIMYRQMVSNS TPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDN +PNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKA- 420
           DLQ PEWL+ASFIFYNEKAQAVRVRVRDCLDTK LGY+YQDVELPWL+TRP PR A +A 
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 -----------ARHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPN 480
                       + PKMK PVTLKSAISTEVKR KKSR +KEK+EEEEILVI+GI+ DPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480

Query: 481 MLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADG 540
             +K DIYINDED++E R D TEFAGSFVNVPHKHGH KKVKTGLRLGI+E+LEDL AD 
Sbjct: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540

Query: 541 DDSIIVTLVPRLGAGLVSIAKIKIEYGD 551
           DDSIIVT++PRLGAGLVSIA+IKIEYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 568

BLAST of CmoCh13G006760 vs. ExPASy TrEMBL
Match: A0A6J1EGP5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434015 PE=3 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 3.5e-284
Identity = 483/568 (85.04%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           MASL PPALST  FRPSSSLRTSKYLP NAARILCSAADG IDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  A------FALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           +      FAL APVQTPNISKCGPPDLPP ADPTNCCPPP+ SI+DF PPAPGKLRVRPA
Sbjct: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120

Query: 121 AHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHL DQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPT ALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300
           HLPPT IDLDFDGVD  IGD+A I+SNLRIMYRQMVSNS TPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDN +PNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRRQ
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKA- 420
           DLQ PEWL+ASFIFYNEKAQAVRVRVRDCLDTK LGY+YQDVEL WL+TRP PR A +A 
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420

Query: 421 -----------ARHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPN 480
                       +  ++KFPVT+KSA+STEVKRAKKSRSKKEKEEEEEILVI+GI+ DPN
Sbjct: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480

Query: 481 MLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADG 540
             +K D+YINDED+ E R D TEFAGSFVNVPHKHGH KKVKTGLRLGI+E+LEDL AD 
Sbjct: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540

Query: 541 DDSIIVTLVPRLGAGLVSIAKIKIEYGD 551
           DDSIIVT++PRLGAGLVSIA+I +EYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 568

BLAST of CmoCh13G006760 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 4.4e-247
Identity = 429/590 (72.71%), Postives = 480/590 (81.36%), Query Frame = 0

Query: 5   SPPALS----TTTF--------RPSSSLRTSKYLPNNAARILCSAADG---NIDRRDVLI 64
           SPPALS    TTTF         P  +LR +KY  + A RILCSA++G   +IDRRD+LI
Sbjct: 4   SPPALSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQNHIDRRDILI 63

Query: 65  GIGAGGLYGAS-----AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPP 124
           G+  GGLYGAS     + A  APVQTPNISKCG PDLPP ADP NCCPPP+ ++VDFRP 
Sbjct: 64  GL--GGLYGASTLSQGSLAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFRPQ 123

Query: 125 APGKLRVRPAAHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGF 184
           APGKLRVRPAAHL DQ+Y++KYKRAVELMK LP+DDPRSF QQANVHCAYCNG YDQVGF
Sbjct: 124 APGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQVGF 183

Query: 185 PVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNS 244
           PVEVQVHNSWLFFPFHRYYL+F+EKILGELIGDP+ ALPFWNYDAPGGM+MPAMYAD  S
Sbjct: 184 PVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEKS 243

Query: 245 SLYDQLRNQNHLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAY 304
           SLYD+LRN+NHLPPTL+DLDF GVDPTIGD+A I+SNL IMYRQMVSNS TP LFFG+AY
Sbjct: 244 SLYDELRNRNHLPPTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFFGNAY 303

Query: 305 VAGDEPSPGGGSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWS 364
           +AGDEPSPGGG VENIPH  VH WCGDN +PN ENMGNFYSAARDPIFY+HHSN+DR+WS
Sbjct: 304 LAGDEPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDRLWS 363

Query: 365 VWKTLGGRRQDLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTR 424
           VWKTLGGRRQDLQN +WLNASFIFY+EKAQAVRVRVRDCLD++ LGYVYQDV+LPWL++R
Sbjct: 364 VWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWLESR 423

Query: 425 PMPRGARKA--------------------------ARHPKMKFPVTLKSAISTEVKRAKK 484
           P PR A +A                           R  K+KFP+TL S IS EVKR K+
Sbjct: 424 PTPRRALRAQKAVAHLPFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISMEVKRPKR 483

Query: 485 SRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHG 544
           SRSKKEK+EEEEI+VI GI+ D N  +KFD++INDED+KE R D TEFAGSFVNVPHKHG
Sbjct: 484 SRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHKHG 543

Query: 545 HNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVSIAKIKIEY 549
           H K VKTGLRLGITELLEDL AD DDS+ VTLVPR G GLVSIA IKIE+
Sbjct: 544 HKKNVKTGLRLGITELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEF 591

BLAST of CmoCh13G006760 vs. NCBI nr
Match: XP_022927080.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ 120
           AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ
Sbjct: 61  AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ 120

Query: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180
           SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH
Sbjct: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180

Query: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240
           RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL
Sbjct: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240

Query: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI 300
           IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI
Sbjct: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI 300

Query: 301 PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE 360
           PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE
Sbjct: 301 PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE 360

Query: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420
           WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK
Sbjct: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420

Query: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR 480
           FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR
Sbjct: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR 480

Query: 481 ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS 540
           ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS
Sbjct: 481 ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS 540

Query: 541 IAKIKIEYGD 551
           IAKIKIEYGD
Sbjct: 541 IAKIKIEYGD 550

BLAST of CmoCh13G006760 vs. NCBI nr
Match: KAG6584006.1 (hypothetical protein SDJN03_19938, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 541/550 (98.36%), Postives = 545/550 (99.09%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           MASLSPPALSTTTFR SSSLRTSKYLPNNAARILCSA DGNIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLSPPALSTTTFRRSSSLRTSKYLPNNAARILCSAGDGNIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ 120
           AFALGAPVQTPNISKCGPPDLPP ADPTNCCPPPSPSI+DFRPPAPGKLRVRPAAHLVDQ
Sbjct: 61  AFALGAPVQTPNISKCGPPDLPPGADPTNCCPPPSPSILDFRPPAPGKLRVRPAAHLVDQ 120

Query: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180
           SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH
Sbjct: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180

Query: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240
           RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL
Sbjct: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240

Query: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI 300
           IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI
Sbjct: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI 300

Query: 301 PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE 360
           PHNTVHTWCGDN +PNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE
Sbjct: 301 PHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE 360

Query: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420
           WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK
Sbjct: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420

Query: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR 480
           FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGI+ DPNMLVKFDIYINDEDEK+NR
Sbjct: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIEFDPNMLVKFDIYINDEDEKDNR 480

Query: 481 ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS 540
           ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS
Sbjct: 481 ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS 540

Query: 541 IAKIKIEYGD 551
           IAKIKIEYGD
Sbjct: 541 IAKIKIEYGD 550

BLAST of CmoCh13G006760 vs. NCBI nr
Match: XP_023520010.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1059.3 bits (2738), Expect = 1.1e-305
Identity = 516/550 (93.82%), Postives = 528/550 (96.00%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           MASLSPPALSTTTFRPSSSLRTSKYLPNN  RILCSAA+GNIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNGTRILCSAANGNIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ 120
           AFALGAPVQTPNISKCGPPDLPP  DPTNCCPPPSPSI+DFRPPAPGKLRVRPAAHLVDQ
Sbjct: 61  AFALGAPVQTPNISKCGPPDLPPGTDPTNCCPPPSPSILDFRPPAPGKLRVRPAAHLVDQ 120

Query: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180
           SYI+KYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH
Sbjct: 121 SYIEKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180

Query: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240
           RYYLYFYEKILGELIGDPT ALPFWNYDAP GMRMPAMYAD NSSLYDQLRNQNHLPPTL
Sbjct: 181 RYYLYFYEKILGELIGDPTFALPFWNYDAPEGMRMPAMYADQNSSLYDQLRNQNHLPPTL 240

Query: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI 300
           IDLDFDGVD  IG++A I++NLRIMYRQMVSNS TPKLFFGS YVAGDEPSPGGGSVENI
Sbjct: 241 IDLDFDGVDSNIGNNAQIRTNLRIMYRQMVSNSRTPKLFFGSPYVAGDEPSPGGGSVENI 300

Query: 301 PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE 360
           PHNTVHTWCGD KKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGG+RQDL+ PE
Sbjct: 301 PHNTVHTWCGDTKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGKRQDLEYPE 360

Query: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420
           WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWL+TRP PR A KAAR PKMK
Sbjct: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLETRPTPRRAGKAARRPKMK 420

Query: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR 480
           FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPN LVKFDIYINDEDEK+NR
Sbjct: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNTLVKFDIYINDEDEKDNR 480

Query: 481 ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSIIVTLVPRLGAGLVS 540
            DITEFAGSFVNVPHKHGH KKVKTGLRLGITE+LEDLGADGDDSIIVTLVPRLGAGLVS
Sbjct: 481 GDITEFAGSFVNVPHKHGHKKKVKTGLRLGITEVLEDLGADGDDSIIVTLVPRLGAGLVS 540

Query: 541 IAKIKIEYGD 551
           IA IKIEYGD
Sbjct: 541 IAGIKIEYGD 550

BLAST of CmoCh13G006760 vs. NCBI nr
Match: XP_023001711.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.3e-297
Identity = 508/568 (89.44%), Postives = 523/568 (92.08%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           M SLS PALSTTTFRPSS   TSKYLPNNAAR+LCSAADG IDRRDVLIGIGAGG+YGAS
Sbjct: 1   MGSLSSPALSTTTFRPSSRPPTSKYLPNNAARVLCSAADGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  A------FALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           A      FAL APVQTPNISKC PPDLP  ADPTNCCPPPSPSIVDFRPP PGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCSPPDLPHGADPTNCCPPPSPSIVDFRPPVPGKLRVRPA 120

Query: 121 AHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHL DQSYIDKYKRAVELMKGLP DDPRSFMQQAN+HCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANIHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPT ALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300
           HLPPTL+DLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDN +PNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKA- 420
           DLQ PEWL+ASFIFYNEKAQAVRVRVRDCLDTK LGY+YQDVELPWL+TRP P  A +A 
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPCRALRAQ 420

Query: 421 -----------ARHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPN 480
                       + PKMKFPVTLKSAISTEVKR KKSRSKKEKEEEEEILVIEGIKLDPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKFPVTLKSAISTEVKRGKKSRSKKEKEEEEEILVIEGIKLDPN 480

Query: 481 MLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADG 540
           MLVKFDIYINDEDEKENRADI+EFAGSFVNVPHKHGH KK+KTGLRLGIT+LLEDL AD 
Sbjct: 481 MLVKFDIYINDEDEKENRADISEFAGSFVNVPHKHGHKKKMKTGLRLGITQLLEDLDADC 540

Query: 541 DDSIIVTLVPRLGAGLVSIAKIKIEYGD 551
           DDSIIVTL+PRLGAGLVSIAKIKIEYGD
Sbjct: 541 DDSIIVTLIPRLGAGLVSIAKIKIEYGD 568

BLAST of CmoCh13G006760 vs. NCBI nr
Match: KAG7019629.1 (hypothetical protein SDJN02_18592, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 998.0 bits (2579), Expect = 3.1e-287
Identity = 489/526 (92.97%), Postives = 496/526 (94.30%), Query Frame = 0

Query: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60
           MASLSPPALSTTTFR SSSLRTSKYLPNNAARILCSA DGNIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLSPPALSTTTFRRSSSLRTSKYLPNNAARILCSAGDGNIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLVDQ 120
           AFA+GAPVQTPNISKCGPPDLPP ADPTNCCPPPSPSI+DFRPPAPGKLRVRPAAHLVDQ
Sbjct: 61  AFAIGAPVQTPNISKCGPPDLPPGADPTNCCPPPSPSILDFRPPAPGKLRVRPAAHLVDQ 120

Query: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180
           SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH
Sbjct: 121 SYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFH 180

Query: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240
           RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL
Sbjct: 181 RYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTL 240

Query: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGGGSVENI 300
           IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSN                     GGSVENI
Sbjct: 241 IDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNR--------------------GGSVENI 300

Query: 301 PHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQDLQNPE 360
           PHNTVHTWCGDN +PNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRRQDLQNPE
Sbjct: 301 PHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQDLQNPE 360

Query: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420
           WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK
Sbjct: 361 WLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKAARHPKMK 420

Query: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPNMLVKFDIYINDEDEKENR 480
           FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGI+ DPNMLVKFDIYINDEDEK+NR
Sbjct: 421 FPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIEFDPNMLVKFDIYINDEDEKDNR 480

Query: 481 ADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADGDDSI 527
           ADITEFAGSFVNVPHK GHNKKVKTGLRLGITELLEDLGAD   S+
Sbjct: 481 ADITEFAGSFVNVPHKQGHNKKVKTGLRLGITELLEDLGADAMASL 506

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P433094.1e-19757.65Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
P433118.2e-17457.01Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q062157.0e-17356.67Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
Q9MB145.5e-16251.05Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=... [more]
Q9ZP191.6e-16155.15Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EMV70.0e+00100.00polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1KJD31.6e-29789.44polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957... [more]
A0A6J1KRC01.5e-28785.92polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957... [more]
A0A6J1EGP53.5e-28485.04polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A1L5JHU34.4e-24772.71Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022927080.10.0e+00100.00polyphenol oxidase, chloroplastic-like [Cucurbita moschata][more]
KAG6584006.10.0e+0098.36hypothetical protein SDJN03_19938, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023520010.11.1e-30593.82polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023001711.13.3e-29789.44polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
KAG7019629.13.1e-28792.97hypothetical protein SDJN02_18592, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 202..207
score: 55.1
coord: 171..188
score: 58.96
coord: 301..312
score: 41.33
coord: 328..346
score: 57.57
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 141..348
e-value: 1.8E-33
score: 116.7
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 329..340
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 171..188
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 421..548
e-value: 2.5E-51
score: 173.2
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 355..405
e-value: 2.7E-24
score: 84.6
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 66..402
e-value: 4.3E-121
score: 406.5
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 70..401
IPR016213Polyphenol oxidasePIRSFPIRSF000290PPO_plantcoord: 10..550
e-value: 5.5E-247
score: 819.2
NoneNo IPR availablePIRSRPIRSR000290-1PIRSR000290-1coord: 66..549
e-value: 3.3E-233
score: 772.9
NoneNo IPR availablePIRSRPIRSR000290-2PIRSR000290-2coord: 66..549
e-value: 3.3E-233
score: 772.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 27..548
NoneNo IPR availablePANTHERPTHR11474:SF94POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 27..548

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G006760.1CmoCh13G006760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity