CmoCh13G005060 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh13G005060 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 5.4e-151
Identity = 270/525 (51.43%), Postives = 367/525 (69.90%), Query Frame = 0

Query: 6   LFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFT--NESASFSSLLQQSIR 65
           LF + L+ +S    S  LQQ FV+C    +S  S PI+   F+    +  F   L+ + +
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVD-NSDVSFPITASFFSPDQNATLFKEELESTAQ 71

Query: 66  NLRFLNTSS-KPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVS-SQSQF 125
           NLR+L  S+ KP+F+  P +E+HVQAAVVCA+K  L LR+RSGGHDYEGLS+V+  ++ F
Sbjct: 72  NLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPF 131

Query: 126 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 185
           +++DLS L  +D+D+++ +A    GAT+GE+YYRI EKS  HGFPAG C ++G+GGH+ G
Sbjct: 132 VIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVG 191

Query: 186 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 245
           G +G++ RK GL ADNV+DA+IVD NG+I+DR  MGE++FWAIRGGGG SFGVIL+WK+K
Sbjct: 192 GAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIK 251

Query: 246 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQ-------EQTIS 305
           LV +P  +T+F V KT +Q   ++  KW++IA KLD+DLF+ V I           +TIS
Sbjct: 252 LVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTIS 311

Query: 306 LSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLK-K 365
           +S+ A FLG   +L+ +M   FPEL L +++ TEM+WI+S+++ AGFP    P+ LL  K
Sbjct: 312 MSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGK 371

Query: 366 SLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHR 425
           SL    FKAKSDFV  PIP  GLEGLW + LEE+    I  PYGG MS+ISE E PFPHR
Sbjct: 372 SLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHR 431

Query: 426 KGNIFGIQYMVSWENGSES-YKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGR 485
            G +F IQ++ +W++G  S  +H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLG N G 
Sbjct: 432 NGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGE 491

Query: 486 NVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSL 518
                +A  WG KYY+ NFERLV++K + DP NFF +EQS+P  +
Sbjct: 492 T----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of CmoCh13G005060 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 274/530 (51.70%), Postives = 370/530 (69.81%), Query Frame = 0

Query: 1   MMGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNE--SASFSSLL 60
           ++ +LL  L  I LS S+    +QQ FV C    +S  S P+    FT++  ++SF   L
Sbjct: 13  LVTLLLLSLSYIPLSFST----IQQDFVMCLVD-NSDASFPMDSSFFTHDLNASSFKLAL 72

Query: 61  QQSIRNLRFLNTSS-KPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSS 120
           + S +NLR+L  S+ KP F+  P +E+HVQAAV+CA+K  L LR+RSGGHDYEGLSYVS 
Sbjct: 73  ETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSE 132

Query: 121 -QSQFILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVG 180
            ++ F+++DLS L  I +DIE+ +A V  GA++GE+YYRI EKS IHGFPAG C ++G+G
Sbjct: 133 METAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIG 192

Query: 181 GHISGGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVIL 240
           GHI GG +G++ RK GL ADNV+DA+IVD +G+I++R  MGE++FWAIRGGGG SFGVIL
Sbjct: 193 GHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVIL 252

Query: 241 SWKLKLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTI-------GVQ 300
           +WK+KLV +P  +T+F V +T +Q   +L  KWQ++A KLDEDLF+ V I         +
Sbjct: 253 AWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSK 312

Query: 301 EQTISLSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQI 360
           E+TIS S+   FLG   +L+ +M   FP+L L +++  E +WI+S+++ AGFP   P + 
Sbjct: 313 ERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEA 372

Query: 361 LLK-KSLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELET 420
           LL  KSL   +FKAKSD+V  PIP  GLEGLW K+LEE+    I  PYGG M++I E ET
Sbjct: 373 LLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETET 432

Query: 421 PFPHRKGNIFGIQYMVSWENGSES-YKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLG 480
           PFPHR G +F IQ++  W++G  S  KH+ W+RE+Y+YME YVSK PR AY+NYRDLDLG
Sbjct: 433 PFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLG 492

Query: 481 RNYGRNVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSL 518
            N G+     +A  WG +Y++ NFERLV +K K DP NFF +EQSIP  L
Sbjct: 493 MN-GKG---SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of CmoCh13G005060 vs. ExPASy Swiss-Prot
Match: Q9FKV2 (Berberine bridge enzyme-like 23 OS=Arabidopsis thaliana OX=3702 GN=At5g44360 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 267/523 (51.05%), Postives = 365/523 (69.79%), Query Frame = 0

Query: 6   LFYLPLI-WLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQSI-R 65
           LF + L+ W++S S S   +  F+ C S++S    I     +   +S  ++   Q  I +
Sbjct: 11  LFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQSLISQ 70

Query: 66  NLRFLN---TSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQ 125
           N RFL    TS KP+ +VTP  ++ +Q +++C++K G+++R +SGGHDYEGLSY+S  S 
Sbjct: 71  NYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLSLHSP 130

Query: 126 FILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHIS 185
           FI+LDL N+ SI+I++  ETA V  GAT+GELYY+IA+ S IHGFPAG+CP+VGVGGH S
Sbjct: 131 FIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVGGHFS 190

Query: 186 GGGFGTLFRKHGLAADNVIDAKIVDFNGRIMD-RNTMGEELFWAIRGGGGASFGVILSWK 245
           GGGFG + RKHGLAADNV+DA+ VD NGRI + R  MGE+LFWAIRGGG ASFGV+LSWK
Sbjct: 191 GGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVVLSWK 250

Query: 246 LKLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVA 305
           +KLV +P K+T F       Q   ++  +WQ+IA +LD++LF+ V + +   ++  +F A
Sbjct: 251 VKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGGSVQTTFQA 310

Query: 306 LFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFF---AGFPIQTPPQILLKKS-- 365
            +LG I+KLIPLMN +FPEL L  ++ +EM WI SI++F    G P++T    LL +   
Sbjct: 311 NYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYFNWKKGQPLET----LLDRGQR 370

Query: 366 LSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRK 425
            ++ +FKAKSDFV  PIP+ GLEG+W +  E E   +I+ P GGKM +I E ETPFPHR+
Sbjct: 371 YNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEVESPIMIMEPLGGKMYEIGETETPFPHRR 430

Query: 426 GNIFGIQYMVSW--ENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGR 485
           GN++ IQYMV W  ++     KH++W+R +Y YM  YVS  PRGAYLNYRDLDLG N G 
Sbjct: 431 GNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNYRDLDLGMNRGV 490

Query: 486 NVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPP 516
           N  +E+A++WG +Y+ SNF+RL  VK K+DP NFF NEQS+PP
Sbjct: 491 NTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPP 529

BLAST of CmoCh13G005060 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 262/531 (49.34%), Postives = 373/531 (70.24%), Query Frame = 0

Query: 1   MMGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVF--TNESASFSSLL 60
           +  IL+ Y  L  ++ +S   +LQ  F+ C    ++    P+ K  F  T   + FS +L
Sbjct: 9   LFSILVLYFSLYTITPTSSLASLQDQFINCVQR-NTHVYFPLEKTFFAPTKNVSMFSQVL 68

Query: 61  QQSIRNLRFLNTS-SKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSS 120
           + + +NLRFL  S  KP F+ +P HESHVQA+++C++K  + LRVRSGGHDYEGLSYVS 
Sbjct: 69  ESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQ 128

Query: 121 -QSQFILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVG 180
               FIL+DLS +  ++I+I+  +A V++GAT+GELYYRIAEKS +HGFPAG C ++G+G
Sbjct: 129 IDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIG 188

Query: 181 GHISGGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVIL 240
           GHI+GG +G++ RK+GL ADNV+DAKIVD NG+++DR  MGE+ FWAIRGG G SFG+IL
Sbjct: 189 GHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIIL 248

Query: 241 SWKLKLVCLPPKLTIFHVQKTPKQG-AIQLFQKWQRIAYKLDEDLFLHVTIGVQ----EQ 300
           +WK+KLV +P  +T+F V KT +Q    ++  KWQR+A KL E+LF+ V   V      +
Sbjct: 249 AWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNK 308

Query: 301 TISLSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILL 360
           T++ S+ ALFLG    L+ +M   FPEL L  ++  EM+W++SI + +GFP  TP  +LL
Sbjct: 309 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLL 368

Query: 361 --KKSLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETP 420
             K       FKAKSDFV  PIP++GL+G++ K+L+E+   +I  PYGG M++I E + P
Sbjct: 369 QGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIP 428

Query: 421 FPHRKGNIFGIQYMVSW-ENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGR 480
           FPHRKG +F +QY+ SW ++     +H++WIR++Y+YM PYVS +PR AY+NYRDLDLGR
Sbjct: 429 FPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGR 488

Query: 481 NYGRNVR--YEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSL 518
           N  ++V+   ++A+VWG  Y+++NF RL+ +K KVDP NFF +EQSIPP +
Sbjct: 489 N-TKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPMM 537

BLAST of CmoCh13G005060 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 5.8e-145
Identity = 252/502 (50.20%), Postives = 356/502 (70.92%), Query Frame = 0

Query: 18  SPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQSIRNLRFLNTS-SKPLF 77
           S + + Q SF++C S   +  +I +SKV+ T    SFSS+L  SI+N RF      KP+ 
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNI-VSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVL 87

Query: 78  LVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQFILLDLSNLHSIDIDI 137
           ++TP   S VQ+AV CA++ G+ +R RSGGHDYEGLSYV +   F++LDL NL SI +D+
Sbjct: 88  ILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYV-THKPFVILDLRNLRSITVDV 147

Query: 138 ETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISGGGFGTLFRKHGLAAD 197
           +  +  V+TGAT+GELYY I +K+    FPAG CPTVGVGGH SGGG+GTL RKHGLAAD
Sbjct: 148 DNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAAD 207

Query: 198 NVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLKLVCLPPKLTIFHVQK 257
           +VIDA++VD  GRI++R  MGE+ FWAIRGGGG+SF V+LSWK+ L+ +P  +T+F+V K
Sbjct: 208 HVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTK 267

Query: 258 TPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALFLGPIEKLIPLMNTQF 317
             +Q A+++  +WQ +A K+ +DLF+ V +   +  +  SF  L+LG ++ L+ ++N +F
Sbjct: 268 FSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVRASFPGLYLGSVKNLLKMVNKEF 327

Query: 318 PELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFKAKSDFVTYPIPQNGL 377
           PEL L+ ++ TEM+WI+S+++FA    + P  +L K++ ++  FKAKSDFV  P+P+  +
Sbjct: 328 PELGLEEDDCTEMSWIESVIWFAELG-EEPINVLTKRTRASLAFKAKSDFVQEPMPKTAI 387

Query: 378 EGLWNKVLEEEGSY--LILTPYGGKMSQISELETPFPHRKGNIFGIQYMVSWENGSESYK 437
             LW ++ E E  +  LI TP+GGKMS+I++ ETPFPHRKGNI+ IQY+  W  G    K
Sbjct: 388 SKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKEK 447

Query: 438 HLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYG-RNVRYEEAEVWGLKYYRSNFER 497
           ++ W+  VY  M  +V+K PRGAY+N RDLDLG   G +  +YEE + WG+KY+++NFER
Sbjct: 448 YMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFER 507

Query: 498 LVRVKTKVDPFNFFWNEQSIPP 516
           LVRVKT VDP +FF +EQSIPP
Sbjct: 508 LVRVKTSVDPSDFFCDEQSIPP 525

BLAST of CmoCh13G005060 vs. ExPASy TrEMBL
Match: A0A6J1EGS9 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111434055 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.1e-303
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0

Query: 2   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS 61
           MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS
Sbjct: 1   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS 60

Query: 62  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 121
           IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF
Sbjct: 61  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 120

Query: 122 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 181
           ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG
Sbjct: 121 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 180

Query: 182 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 241
           GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK
Sbjct: 181 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 240

Query: 242 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 301
           LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF
Sbjct: 241 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 300

Query: 302 LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 361
           LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK
Sbjct: 301 LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 360

Query: 362 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 421
           AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ
Sbjct: 361 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 420

Query: 422 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV 481
           YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV
Sbjct: 421 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV 480

Query: 482 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 521
           WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS
Sbjct: 481 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 519

BLAST of CmoCh13G005060 vs. ExPASy TrEMBL
Match: A0A6J1KH65 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111495709 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 7.8e-294
Identity = 502/519 (96.72%), Postives = 510/519 (98.27%), Query Frame = 0

Query: 2   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS 61
           MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLL QS
Sbjct: 1   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLHQS 60

Query: 62  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 121
           IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCA+KKGLQLRVRSGGHDYEGLSY+SSQSQF
Sbjct: 61  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAKKKGLQLRVRSGGHDYEGLSYLSSQSQF 120

Query: 122 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 181
           I+LDL+NL SID+DIETETASVETGATLGE YYRIAEKSLIHGFPAGSCPTVGVGGHISG
Sbjct: 121 IVLDLTNLRSIDVDIETETASVETGATLGEFYYRIAEKSLIHGFPAGSCPTVGVGGHISG 180

Query: 182 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 241
           GGFGTLFRKHGLAAD VIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK
Sbjct: 181 GGFGTLFRKHGLAADKVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 240

Query: 242 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 301
           LV LPPKLTIFHVQKTPKQGA+QLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF
Sbjct: 241 LVSLPPKLTIFHVQKTPKQGAMQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 300

Query: 302 LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 361
           LGPIEKLIPLMNTQFPEL LQRENYTEMNWIQSILFFAGFPIQTPPQILLKK LSNAFFK
Sbjct: 301 LGPIEKLIPLMNTQFPELGLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKPLSNAFFK 360

Query: 362 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 421
           AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ
Sbjct: 361 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 420

Query: 422 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV 481
           YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEA+V
Sbjct: 421 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAKV 480

Query: 482 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 521
           WGLKYY+SNFERLVRVKTKVDP NFFWNEQSIP SLYRS
Sbjct: 481 WGLKYYKSNFERLVRVKTKVDPSNFFWNEQSIPTSLYRS 519

BLAST of CmoCh13G005060 vs. ExPASy TrEMBL
Match: A0A1S3BH69 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103489514 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 9.4e-231
Identity = 410/535 (76.64%), Postives = 461/535 (86.17%), Query Frame = 0

Query: 5   LLFYLPLIWLSSSSPSHALQ-QSFVECFSS---YSSPQSIPISKVVFTNE-SASFSSLLQ 64
           LLFYLP IWLSSS  S++LQ +SF+ECF+S   Y SPQSIPISKVVFT++ SA FSSLLQ
Sbjct: 14  LLFYLPFIWLSSS--SYSLQAESFLECFNSTSYYYSPQSIPISKVVFTSKSSALFSSLLQ 73

Query: 65  QSIRNLRFLNTS-SKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSS- 124
            SI+NLRFLNTS  KPLFLVTPFH+SHVQ+A+VCA KKGLQ+RVRSGGHDYEG+SYVSS 
Sbjct: 74  SSIKNLRFLNTSLQKPLFLVTPFHQSHVQSAIVCANKKGLQIRVRSGGHDYEGISYVSSD 133

Query: 125 QSQFILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGG 184
           QSQFILLD  N  SIDID++TETA VE GATLGELYYRIAEKS IHGFPAGSCPTVG+GG
Sbjct: 134 QSQFILLDFINFRSIDIDMKTETAIVEAGATLGELYYRIAEKSPIHGFPAGSCPTVGMGG 193

Query: 185 HISGGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILS 244
           H+ GGGFGTLFRK+GLAADNVIDAKIVD NGRIMDRN+MGE+LFWAIRGGGGASFGVILS
Sbjct: 194 HVGGGGFGTLFRKYGLAADNVIDAKIVDVNGRIMDRNSMGEDLFWAIRGGGGASFGVILS 253

Query: 245 WKLKLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGV--------- 304
           WKLKLV +P  +T+F VQKT +QGAI LFQKWQRIA+KL EDLFLHVT+GV         
Sbjct: 254 WKLKLVYVPSNVTVFVVQKTLEQGAIHLFQKWQRIAHKLHEDLFLHVTMGVIDEQNKTPN 313

Query: 305 -QEQTISLSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPP 364
              +TI +SFV+LFLG IE+LIPLMN+ FPEL L+R N TEM+WIQS+LFFAG  I+TPP
Sbjct: 314 MSSKTILISFVSLFLGRIERLIPLMNSHFPELGLERNNCTEMSWIQSVLFFAGISIETPP 373

Query: 365 QILLKKSLSN--AFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISE 424
           ++LLK+  ++   FFKAKSDFV  PIPQ G EGLW K+LEEE SYLILTPYGGKM QIS+
Sbjct: 374 KVLLKRPPTSKMLFFKAKSDFVISPIPQIGFEGLWTKMLEEEASYLILTPYGGKMRQISD 433

Query: 425 LETPFPHRKGNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLD 484
            +TPFPHRKGNIFGIQYMV+WEN +E+Y+HLSWIREVY YMEPYVSK PR AYLNYRDLD
Sbjct: 434 FDTPFPHRKGNIFGIQYMVTWENANETYRHLSWIREVYDYMEPYVSKHPRAAYLNYRDLD 493

Query: 485 LGRNYGRNVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 521
           LGRN GRN  YEEA+VWGLKYY+SNF+RLVRVKTKVDP NFFWNEQSIP S+Y S
Sbjct: 494 LGRNCGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKVDPLNFFWNEQSIPTSVYHS 546

BLAST of CmoCh13G005060 vs. ExPASy TrEMBL
Match: A0A5A7TYX1 (Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold437G00060 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.8e-219
Identity = 386/520 (74.23%), Postives = 446/520 (85.77%), Query Frame = 0

Query: 5   LLFYLPLIWLSSSSPSHALQQSFVECFSSYS-SPQSIPISKVVFTNESASFSSLLQQSIR 64
           LLF + LIWL SSS S  LQ +F+ECF+S S S  SIP+S+VVFTNESASFSSL + SIR
Sbjct: 13  LLFCVSLIWLCSSSDS--LQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLFRLSIR 72

Query: 65  NLRFLNTS-SKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQFI 124
           NLRFL T+  KPLFLVTPFHESHVQAA+VCA++KGLQ+RVRSGGHDYEGLSYVSSQ+ FI
Sbjct: 73  NLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYVSSQAPFI 132

Query: 125 LLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISGG 184
           ++DL NL SI IDIE ETASV TGA LGELYYRIA+KS IHGFPAGSCPTVGVGGHISGG
Sbjct: 133 VIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGG 192

Query: 185 GFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLKL 244
           GFGTLFRK+GLAADNVIDAKI+DFNGRIMDR++MGE+LFWAIRGGGGASFGVILSWKLKL
Sbjct: 193 GFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKL 252

Query: 245 VCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGV-----QEQTISLSF 304
           V +P  +T+F+VQ+T +QGA  LFQKWQ I++KLD+D+FLHVT  V      ++TI LSF
Sbjct: 253 VSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFPSKKTIRLSF 312

Query: 305 VALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKS--- 364
            +LFLGPIE+LIP+M T+F EL L+R +  EM+WIQS+LFFA F I TP ++L+++S   
Sbjct: 313 TSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEVLMERSSPQ 372

Query: 365 LSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRK 424
           +S+AFF AKSD+V  PI Q GLEGLW K+LEEE S LILTPYGGKMSQISE +TPFPHR+
Sbjct: 373 ISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISESQTPFPHRE 432

Query: 425 GNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNV 484
           G IFGIQY+ +W+N +E+ KHLSWIREVYAYM+PYVSK PR AYLNYRDLDLGRNYGRN 
Sbjct: 433 GRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDLGRNYGRNT 492

Query: 485 RYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIP 515
            YEEA+VWGLKY+  NFERLVRVKTKVDP NFFWNEQSIP
Sbjct: 493 SYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 530

BLAST of CmoCh13G005060 vs. ExPASy TrEMBL
Match: A0A1S3BE11 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103488588 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.8e-219
Identity = 386/520 (74.23%), Postives = 446/520 (85.77%), Query Frame = 0

Query: 5   LLFYLPLIWLSSSSPSHALQQSFVECFSSYS-SPQSIPISKVVFTNESASFSSLLQQSIR 64
           LLF + LIWL SSS S  LQ +F+ECF+S S S  SIP+S+VVFTNESASFSSL + SIR
Sbjct: 13  LLFCVSLIWLCSSSDS--LQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLFRLSIR 72

Query: 65  NLRFLNTS-SKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQFI 124
           NLRFL T+  KPLFLVTPFHESHVQAA+VCA++KGLQ+RVRSGGHDYEGLSYVSSQ+ FI
Sbjct: 73  NLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYVSSQAPFI 132

Query: 125 LLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISGG 184
           ++DL NL SI IDIE ETASV TGA LGELYYRIA+KS IHGFPAGSCPTVGVGGHISGG
Sbjct: 133 VIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGG 192

Query: 185 GFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLKL 244
           GFGTLFRK+GLAADNVIDAKI+DFNGRIMDR++MGE+LFWAIRGGGGASFGVILSWKLKL
Sbjct: 193 GFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKL 252

Query: 245 VCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGV-----QEQTISLSF 304
           V +P  +T+F+VQ+T +QGA  LFQKWQ I++KLD+D+FLHVT  V      ++TI LSF
Sbjct: 253 VSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFPSKKTIRLSF 312

Query: 305 VALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKS--- 364
            +LFLGPIE+LIP+M T+F EL L+R +  EM+WIQS+LFFA F I TP ++L+++S   
Sbjct: 313 TSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEVLMERSSPQ 372

Query: 365 LSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRK 424
           +S+AFF AKSD+V  PI Q GLEGLW K+LEEE S LILTPYGGKMSQISE +TPFPHR+
Sbjct: 373 ISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISESQTPFPHRE 432

Query: 425 GNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNV 484
           G IFGIQY+ +W+N +E+ KHLSWIREVYAYM+PYVSK PR AYLNYRDLDLGRNYGRN 
Sbjct: 433 GRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDLGRNYGRNT 492

Query: 485 RYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIP 515
            YEEA+VWGLKY+  NFERLVRVKTKVDP NFFWNEQSIP
Sbjct: 493 SYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 530

BLAST of CmoCh13G005060 vs. NCBI nr
Match: XP_022927116.1 (berberine bridge enzyme-like 15 [Cucurbita moschata])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.2e-303
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0

Query: 2   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS 61
           MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS
Sbjct: 1   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS 60

Query: 62  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 121
           IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF
Sbjct: 61  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 120

Query: 122 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 181
           ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG
Sbjct: 121 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 180

Query: 182 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 241
           GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK
Sbjct: 181 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 240

Query: 242 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 301
           LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF
Sbjct: 241 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 300

Query: 302 LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 361
           LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK
Sbjct: 301 LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 360

Query: 362 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 421
           AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ
Sbjct: 361 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 420

Query: 422 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV 481
           YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV
Sbjct: 421 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV 480

Query: 482 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 521
           WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS
Sbjct: 481 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 519

BLAST of CmoCh13G005060 vs. NCBI nr
Match: KAG7019453.1 (Berberine bridge enzyme-like 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.2e-300
Identity = 514/519 (99.04%), Postives = 516/519 (99.42%), Query Frame = 0

Query: 2   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS 61
           MGILLFYLPLIWLSSSSPSHALQQSFVECFSS+SSPQSIPISKVVFTNESASFSSLL QS
Sbjct: 1   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSHSSPQSIPISKVVFTNESASFSSLLHQS 60

Query: 62  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 121
           IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF
Sbjct: 61  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 120

Query: 122 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 181
           ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG
Sbjct: 121 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 180

Query: 182 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 241
           GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK
Sbjct: 181 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 240

Query: 242 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 301
           LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF
Sbjct: 241 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 300

Query: 302 LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 361
           LGPIEKLIPLMNTQFPEL LQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK
Sbjct: 301 LGPIEKLIPLMNTQFPELGLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 360

Query: 362 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 421
           AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ
Sbjct: 361 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 420

Query: 422 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV 481
           YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEA+V
Sbjct: 421 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAKV 480

Query: 482 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 521
           WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIP SLYRS
Sbjct: 481 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPTSLYRS 519

BLAST of CmoCh13G005060 vs. NCBI nr
Match: XP_023520227.1 (berberine bridge enzyme-like 15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.1e-297
Identity = 509/519 (98.07%), Postives = 513/519 (98.84%), Query Frame = 0

Query: 2   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS 61
           MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLL QS
Sbjct: 1   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLHQS 60

Query: 62  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 121
           IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF
Sbjct: 61  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 120

Query: 122 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 181
           I+LDL+NL SIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG
Sbjct: 121 IVLDLTNLRSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 180

Query: 182 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 241
           GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRN MGEELFWAIRGGGGASFGVILSWKLK
Sbjct: 181 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNAMGEELFWAIRGGGGASFGVILSWKLK 240

Query: 242 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 301
           LV LPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF
Sbjct: 241 LVSLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 300

Query: 302 LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 361
           LGPIEKLIPLMNTQFPEL LQRENYTE+NWIQSILFFAGFPIQTPPQILLKKSLSNAFFK
Sbjct: 301 LGPIEKLIPLMNTQFPELGLQRENYTELNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 360

Query: 362 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 421
           AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQIS LETPFPHRKGNIFGIQ
Sbjct: 361 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISSLETPFPHRKGNIFGIQ 420

Query: 422 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV 481
           YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEA+V
Sbjct: 421 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAKV 480

Query: 482 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 521
           WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS
Sbjct: 481 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 519

BLAST of CmoCh13G005060 vs. NCBI nr
Match: XP_023001637.1 (berberine bridge enzyme-like 15 [Cucurbita maxima])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.6e-293
Identity = 502/519 (96.72%), Postives = 510/519 (98.27%), Query Frame = 0

Query: 2   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQS 61
           MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLL QS
Sbjct: 1   MGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLHQS 60

Query: 62  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 121
           IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCA+KKGLQLRVRSGGHDYEGLSY+SSQSQF
Sbjct: 61  IRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAKKKGLQLRVRSGGHDYEGLSYLSSQSQF 120

Query: 122 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 181
           I+LDL+NL SID+DIETETASVETGATLGE YYRIAEKSLIHGFPAGSCPTVGVGGHISG
Sbjct: 121 IVLDLTNLRSIDVDIETETASVETGATLGEFYYRIAEKSLIHGFPAGSCPTVGVGGHISG 180

Query: 182 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 241
           GGFGTLFRKHGLAAD VIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK
Sbjct: 181 GGFGTLFRKHGLAADKVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 240

Query: 242 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 301
           LV LPPKLTIFHVQKTPKQGA+QLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF
Sbjct: 241 LVSLPPKLTIFHVQKTPKQGAMQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALF 300

Query: 302 LGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFK 361
           LGPIEKLIPLMNTQFPEL LQRENYTEMNWIQSILFFAGFPIQTPPQILLKK LSNAFFK
Sbjct: 301 LGPIEKLIPLMNTQFPELGLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKPLSNAFFK 360

Query: 362 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 421
           AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ
Sbjct: 361 AKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGIQ 420

Query: 422 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAEV 481
           YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEA+V
Sbjct: 421 YMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAKV 480

Query: 482 WGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 521
           WGLKYY+SNFERLVRVKTKVDP NFFWNEQSIP SLYRS
Sbjct: 481 WGLKYYKSNFERLVRVKTKVDPSNFFWNEQSIPTSLYRS 519

BLAST of CmoCh13G005060 vs. NCBI nr
Match: KAG6583832.1 (Berberine bridge enzyme-like 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.5e-288
Identity = 500/520 (96.15%), Postives = 502/520 (96.54%), Query Frame = 0

Query: 1   MMGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQ 60
           MMGILLFYLPLIWLSSSSPSHALQQSFVECFSS+SSPQSIPISKVVFTNESASFSSLL Q
Sbjct: 1   MMGILLFYLPLIWLSSSSPSHALQQSFVECFSSHSSPQSIPISKVVFTNESASFSSLLHQ 60

Query: 61  SIRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQ 120
           SIRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKK              GLSYVSSQSQ
Sbjct: 61  SIRNLRFLNTSSKPLFLVTPFHESHVQAAVVCAQKK--------------GLSYVSSQSQ 120

Query: 121 FILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHIS 180
           FILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHIS
Sbjct: 121 FILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHIS 180

Query: 181 GGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKL 240
           GGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKL
Sbjct: 181 GGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKL 240

Query: 241 KLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVAL 300
           KLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVAL
Sbjct: 241 KLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVAL 300

Query: 301 FLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFF 360
           FLGP EKLIPLMNTQFPEL LQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFF
Sbjct: 301 FLGPTEKLIPLMNTQFPELGLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFF 360

Query: 361 KAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGI 420
           KAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGI
Sbjct: 361 KAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRKGNIFGI 420

Query: 421 QYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAE 480
           QYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEA+
Sbjct: 421 QYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGRNVRYEEAK 480

Query: 481 VWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 521
           VWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIP SLYRS
Sbjct: 481 VWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPTSLYRS 506

BLAST of CmoCh13G005060 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 535.8 bits (1379), Expect = 3.8e-152
Identity = 270/525 (51.43%), Postives = 367/525 (69.90%), Query Frame = 0

Query: 6   LFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFT--NESASFSSLLQQSIR 65
           LF + L+ +S    S  LQQ FV+C    +S  S PI+   F+    +  F   L+ + +
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVD-NSDVSFPITASFFSPDQNATLFKEELESTAQ 71

Query: 66  NLRFLNTSS-KPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVS-SQSQF 125
           NLR+L  S+ KP+F+  P +E+HVQAAVVCA+K  L LR+RSGGHDYEGLS+V+  ++ F
Sbjct: 72  NLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPF 131

Query: 126 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 185
           +++DLS L  +D+D+++ +A    GAT+GE+YYRI EKS  HGFPAG C ++G+GGH+ G
Sbjct: 132 VIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVG 191

Query: 186 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 245
           G +G++ RK GL ADNV+DA+IVD NG+I+DR  MGE++FWAIRGGGG SFGVIL+WK+K
Sbjct: 192 GAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIK 251

Query: 246 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQ-------EQTIS 305
           LV +P  +T+F V KT +Q   ++  KW++IA KLD+DLF+ V I           +TIS
Sbjct: 252 LVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTIS 311

Query: 306 LSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLK-K 365
           +S+ A FLG   +L+ +M   FPEL L +++ TEM+WI+S+++ AGFP    P+ LL  K
Sbjct: 312 MSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGK 371

Query: 366 SLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHR 425
           SL    FKAKSDFV  PIP  GLEGLW + LEE+    I  PYGG MS+ISE E PFPHR
Sbjct: 372 SLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHR 431

Query: 426 KGNIFGIQYMVSWENGSES-YKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGR 485
            G +F IQ++ +W++G  S  +H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLG N G 
Sbjct: 432 NGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGE 491

Query: 486 NVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSL 518
                +A  WG KYY+ NFERLV++K + DP NFF +EQS+P  +
Sbjct: 492 T----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of CmoCh13G005060 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.0e-149
Identity = 274/530 (51.70%), Postives = 370/530 (69.81%), Query Frame = 0

Query: 1   MMGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNE--SASFSSLL 60
           ++ +LL  L  I LS S+    +QQ FV C    +S  S P+    FT++  ++SF   L
Sbjct: 13  LVTLLLLSLSYIPLSFST----IQQDFVMCLVD-NSDASFPMDSSFFTHDLNASSFKLAL 72

Query: 61  QQSIRNLRFLNTSS-KPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSS 120
           + S +NLR+L  S+ KP F+  P +E+HVQAAV+CA+K  L LR+RSGGHDYEGLSYVS 
Sbjct: 73  ETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSE 132

Query: 121 -QSQFILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVG 180
            ++ F+++DLS L  I +DIE+ +A V  GA++GE+YYRI EKS IHGFPAG C ++G+G
Sbjct: 133 METAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIG 192

Query: 181 GHISGGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVIL 240
           GHI GG +G++ RK GL ADNV+DA+IVD +G+I++R  MGE++FWAIRGGGG SFGVIL
Sbjct: 193 GHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVIL 252

Query: 241 SWKLKLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTI-------GVQ 300
           +WK+KLV +P  +T+F V +T +Q   +L  KWQ++A KLDEDLF+ V I         +
Sbjct: 253 AWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSK 312

Query: 301 EQTISLSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQI 360
           E+TIS S+   FLG   +L+ +M   FP+L L +++  E +WI+S+++ AGFP   P + 
Sbjct: 313 ERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEA 372

Query: 361 LLK-KSLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELET 420
           LL  KSL   +FKAKSD+V  PIP  GLEGLW K+LEE+    I  PYGG M++I E ET
Sbjct: 373 LLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETET 432

Query: 421 PFPHRKGNIFGIQYMVSWENGSES-YKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLG 480
           PFPHR G +F IQ++  W++G  S  KH+ W+RE+Y+YME YVSK PR AY+NYRDLDLG
Sbjct: 433 PFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLG 492

Query: 481 RNYGRNVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSL 518
            N G+     +A  WG +Y++ NFERLV +K K DP NFF +EQSIP  L
Sbjct: 493 MN-GKG---SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of CmoCh13G005060 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.0e-149
Identity = 267/523 (51.05%), Postives = 365/523 (69.79%), Query Frame = 0

Query: 6   LFYLPLI-WLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQSI-R 65
           LF + L+ W++S S S   +  F+ C S++S    I     +   +S  ++   Q  I +
Sbjct: 11  LFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQSLISQ 70

Query: 66  NLRFLN---TSSKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQ 125
           N RFL    TS KP+ +VTP  ++ +Q +++C++K G+++R +SGGHDYEGLSY+S  S 
Sbjct: 71  NYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLSLHSP 130

Query: 126 FILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHIS 185
           FI+LDL N+ SI+I++  ETA V  GAT+GELYY+IA+ S IHGFPAG+CP+VGVGGH S
Sbjct: 131 FIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVGGHFS 190

Query: 186 GGGFGTLFRKHGLAADNVIDAKIVDFNGRIMD-RNTMGEELFWAIRGGGGASFGVILSWK 245
           GGGFG + RKHGLAADNV+DA+ VD NGRI + R  MGE+LFWAIRGGG ASFGV+LSWK
Sbjct: 191 GGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVVLSWK 250

Query: 246 LKLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVA 305
           +KLV +P K+T F       Q   ++  +WQ+IA +LD++LF+ V + +   ++  +F A
Sbjct: 251 VKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGGSVQTTFQA 310

Query: 306 LFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFF---AGFPIQTPPQILLKKS-- 365
            +LG I+KLIPLMN +FPEL L  ++ +EM WI SI++F    G P++T    LL +   
Sbjct: 311 NYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYFNWKKGQPLET----LLDRGQR 370

Query: 366 LSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPFPHRK 425
            ++ +FKAKSDFV  PIP+ GLEG+W +  E E   +I+ P GGKM +I E ETPFPHR+
Sbjct: 371 YNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEVESPIMIMEPLGGKMYEIGETETPFPHRR 430

Query: 426 GNIFGIQYMVSW--ENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYGR 485
           GN++ IQYMV W  ++     KH++W+R +Y YM  YVS  PRGAYLNYRDLDLG N G 
Sbjct: 431 GNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNYRDLDLGMNRGV 490

Query: 486 NVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPP 516
           N  +E+A++WG +Y+ SNF+RL  VK K+DP NFF NEQS+PP
Sbjct: 491 NTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPP 529

BLAST of CmoCh13G005060 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 521.5 bits (1342), Expect = 7.5e-148
Identity = 262/531 (49.34%), Postives = 373/531 (70.24%), Query Frame = 0

Query: 1   MMGILLFYLPLIWLSSSSPSHALQQSFVECFSSYSSPQSIPISKVVF--TNESASFSSLL 60
           +  IL+ Y  L  ++ +S   +LQ  F+ C    ++    P+ K  F  T   + FS +L
Sbjct: 9   LFSILVLYFSLYTITPTSSLASLQDQFINCVQR-NTHVYFPLEKTFFAPTKNVSMFSQVL 68

Query: 61  QQSIRNLRFLNTS-SKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSS 120
           + + +NLRFL  S  KP F+ +P HESHVQA+++C++K  + LRVRSGGHDYEGLSYVS 
Sbjct: 69  ESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQ 128

Query: 121 -QSQFILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVG 180
               FIL+DLS +  ++I+I+  +A V++GAT+GELYYRIAEKS +HGFPAG C ++G+G
Sbjct: 129 IDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIG 188

Query: 181 GHISGGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVIL 240
           GHI+GG +G++ RK+GL ADNV+DAKIVD NG+++DR  MGE+ FWAIRGG G SFG+IL
Sbjct: 189 GHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIIL 248

Query: 241 SWKLKLVCLPPKLTIFHVQKTPKQG-AIQLFQKWQRIAYKLDEDLFLHVTIGVQ----EQ 300
           +WK+KLV +P  +T+F V KT +Q    ++  KWQR+A KL E+LF+ V   V      +
Sbjct: 249 AWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNK 308

Query: 301 TISLSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILL 360
           T++ S+ ALFLG    L+ +M   FPEL L  ++  EM+W++SI + +GFP  TP  +LL
Sbjct: 309 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLL 368

Query: 361 --KKSLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETP 420
             K       FKAKSDFV  PIP++GL+G++ K+L+E+   +I  PYGG M++I E + P
Sbjct: 369 QGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIP 428

Query: 421 FPHRKGNIFGIQYMVSW-ENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGR 480
           FPHRKG +F +QY+ SW ++     +H++WIR++Y+YM PYVS +PR AY+NYRDLDLGR
Sbjct: 429 FPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGR 488

Query: 481 NYGRNVR--YEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSL 518
           N  ++V+   ++A+VWG  Y+++NF RL+ +K KVDP NFF +EQSIPP +
Sbjct: 489 N-TKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPMM 537

BLAST of CmoCh13G005060 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.8 bits (1327), Expect = 4.1e-146
Identity = 252/502 (50.20%), Postives = 356/502 (70.92%), Query Frame = 0

Query: 18  SPSHALQQSFVECFSSYSSPQSIPISKVVFTNESASFSSLLQQSIRNLRFLNTS-SKPLF 77
           S + + Q SF++C S   +  +I +SKV+ T    SFSS+L  SI+N RF      KP+ 
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNI-VSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVL 87

Query: 78  LVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQFILLDLSNLHSIDIDI 137
           ++TP   S VQ+AV CA++ G+ +R RSGGHDYEGLSYV +   F++LDL NL SI +D+
Sbjct: 88  ILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYV-THKPFVILDLRNLRSITVDV 147

Query: 138 ETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISGGGFGTLFRKHGLAAD 197
           +  +  V+TGAT+GELYY I +K+    FPAG CPTVGVGGH SGGG+GTL RKHGLAAD
Sbjct: 148 DNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAAD 207

Query: 198 NVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLKLVCLPPKLTIFHVQK 257
           +VIDA++VD  GRI++R  MGE+ FWAIRGGGG+SF V+LSWK+ L+ +P  +T+F+V K
Sbjct: 208 HVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTK 267

Query: 258 TPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQTISLSFVALFLGPIEKLIPLMNTQF 317
             +Q A+++  +WQ +A K+ +DLF+ V +   +  +  SF  L+LG ++ L+ ++N +F
Sbjct: 268 FSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVRASFPGLYLGSVKNLLKMVNKEF 327

Query: 318 PELVLQRENYTEMNWIQSILFFAGFPIQTPPQILLKKSLSNAFFKAKSDFVTYPIPQNGL 377
           PEL L+ ++ TEM+WI+S+++FA    + P  +L K++ ++  FKAKSDFV  P+P+  +
Sbjct: 328 PELGLEEDDCTEMSWIESVIWFAELG-EEPINVLTKRTRASLAFKAKSDFVQEPMPKTAI 387

Query: 378 EGLWNKVLEEEGSY--LILTPYGGKMSQISELETPFPHRKGNIFGIQYMVSWENGSESYK 437
             LW ++ E E  +  LI TP+GGKMS+I++ ETPFPHRKGNI+ IQY+  W  G    K
Sbjct: 388 SKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKEK 447

Query: 438 HLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNYG-RNVRYEEAEVWGLKYYRSNFER 497
           ++ W+  VY  M  +V+K PRGAY+N RDLDLG   G +  +YEE + WG+KY+++NFER
Sbjct: 448 YMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFER 507

Query: 498 LVRVKTKVDPFNFFWNEQSIPP 516
           LVRVKT VDP +FF +EQSIPP
Sbjct: 508 LVRVKTSVDPSDFFCDEQSIPP 525