CmoCh13G000110 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh13G000110
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	PseudoU_synth_1 domain-containing protein
Location	Cmo_Chr13: 145338 .. 150206 (+)
RNA-Seq Expression	CmoCh13G000110
Synteny	CmoCh13G000110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATGCCATGGCAGTCTCTTCTCTGCGATTCCCGCTTTCCCCATGGGCAAACAAAACTTCATTTCCATGCTTCCGTTTTCCCTACCCTAATTTCGTTTCGTGGACCTCTCGTCTGAGTCCTAGACTGCTGTGCTGTTCCTCATCTTCTACTGCTTCCACCGATGCTCTTTCCACTCAGTGCGCCCCAGACCAGCTCCGGAAATGGGAAGCTTTTCGGAAGAAGAAGGTCGTTATGCGGATTGGCTATGTTGGGACTGATTATAGAGGTCTCATTTGATATTACTCTCTGGTTCTCTTGACGACCCTTTTGTGAATCCTTGACCAGTTCATTAATTCTTGAGTTTAAAAAATGCAGGGCTGCAAATGCAACGCGAGCAATCATCACATCCAAGTGAGCTTTTTTCATCATTTCTTTTTACATCATTTACATAATGTCATGAAAATTCCTGTCGGGGATTGGCAATGACATCCGCCGGATGTTCTGTTGCTGTCCTTGATTTGCATACTTTCGTAGTGAATGTGATGGTGGTTGCTCAATTACTTACTTAGCCAAGTGTAAAGTACAACAACTATTTATAACAATTTAGTTCACAGTGACTAACAGAAACAGTTGAAATTCACTGACCAATTGTGATATGAACCAAGACATCGATCATGCAATATAAACTTACAAATGAGTTATGGTATTTCACTAAGGTTACATTTACATCATGGCTAATTATGACCATAAAGTATACATAGTAGTCATAAGATATGAAAGTTACAACTCTTGAAAGGCTTCATAGAAGGTTATGGGTTTTCATTTTGAGGCTATATAAAGCCATGTATGTTATCTTTGCAAGGGAGATTTGGAAAATGTAGTAAAAACAGCATTTTCGCTTTGTTTTAGCCTGGGGCTAAGTTTCTTTGCAATTCTATTAAGTCTAGATTGCATGTTTAGAATCATTCAAGTTTGACATGATCGACCTTGCTTGTGGAGTGACTCGAATCTCAATCAAGTGTTCTTGCCTTGAGATATCTGATTTGCCTTGGGATATCTGATCAACAAGGTAATCTGAATTTTATTTTCCCTTGGAGTGATTCCTTATCATTAGCGCTAAGTGTTCTTACCTTTCCTAAGTCGAGGGATAAGTTTCTTTGTAATTCTATGTAGTTTAGGTTGCATGTTTAGAATCATTCAAGCGTGATCCGAATCTCAGACAAGTGTTCTTGCCTTGAGATATTTGATATCCGAATCTCATTGATAAAGCATTCATGGTTCAAGCTATCTATTGCAACTAGCGCTCGAAACCTTGGAACTTATTATCAGACGTTCCTATGATGTACTGACAGATTGGATTCCATGTATTTCAGACAATGTAATCTAAGAGGCAAGTTTTCAAGGGGAACTTTGTAACATGCTTGCTGTTACATGATTTGGGAGAACTAGAGATGCGTGGCAGAATAGTTACTGCTAAAAGAGTACATAAAAAGGACATGTATCAGCAACACTAATGCCATTAAACTTTGTCAGCGATAATGAAGTGATGCACGCACCATGCATTAGTTACTAACTACAATAAATTTAAAATATAACTGCAGACTGAACCAACAGAAATAGTTGAGCTTAATATGCATTCAGCTGATCAATATCTGATTAAATACACTTAACTTCTTAACCAACCTTGATATATTGTTTTATGTATATTAATCTCATAGGATCTGTCGTAGATTTCCATTTATTTTATTGGATAAGCATTTCTTTGTCTTTCAATGTTTCTTTTACCATTAAAAAATGTTAAGTGCCCATGTTGGTTATGATAATAAGAAATTAACCTCGTACAGTTCAAAGTACCCAGCTAATGCATGGATGGAATGTCCTAAAGCATTACAGTTTTAATGTGTAGCAATTGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAGATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTAAATATCCTGAATGTCTTTGTTGCATCTCATTTTATGACTTTTGTAATCTTATTGGTTGTTTTGATTTGTTTTTTCTTTCTCTTTCATTTTCAAGGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGATAGTGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATCAACTTTTATCTTCCTGAAAATATCAGGGTTTTTGGCATTGTACCTTCACAGAGGTAAGTGCTCTTTTTTTTTTTTCTTTTTTTTTGGGTGCTTTGAAGTGAGTAAATGAAGTTTCAGCGTTAGCATGATGCGTTTGTATGTTTTTACTACAATGTGGTAACTTTACATCATTTTTTTCACTTGTCAAGGAGCTTTGATCCTAGAAGGGAGTGTGATGTTCGGAAGTATACTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAGCTCACAACTACTGAAATTGATTATCACATCTCAGAATTTAAGGATATATTAAATTCGTTTCAGGTAGGTTTTTATCTCCTTCATTTAGTTACATAAAGATCAGTTTGTACCTCCCTTCACTGCAAGATACATGATTTGTTATTATTTGATAGTCTAGTGCTGTTATTTGGAAGACAATTGTATGGCGTTGAAGAACATAGGTGGATTCTTTTTGCGTACAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGGATTCGAACCAACCAATCTCCAGGATATGTCCACAAGTCAGGAGATGGGGTATCATCTAGTGATTTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAAACGATATTTTTGTTGGAGGAATCAAGGAACCAAATCAACATTCTCCAAGCTTAAGTGGTAGTTATGAGAATACATTTGAAGTATGCAGTAAACTTGGAAATGGATCAGAGCTTCCACAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAAATCCTATACTGTTCTTGTGGGAAGCTGGAAACATCTCTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCTTTCATGTTGCATCAAGTTAGTCTTTCTCTAACTTTGTTTTCTTTGGTGCGTAATTTGTTCTTATTTCCGTTGTCCATGTCTACTTCATTTATTCTTATTTCCGTTGGCAGCTCTGTCGCTTAGATTTGGGTGGTTTTCCGTTCTAACACCTCTTGTAGCTTAGGTCATGATTCTGGTCACATGGGAGCCAAAACTTAGTATATAACTGTTCTTTTGTCCCCCACTCGATAATTTCTTGTCAGCCTTATTTCATTTTTGAACTGTTTTGCCCCTTTGGAAATCAATGCTCAGTGGCCTAGTGGGGTTTTTCTGTGCTAGTAGTCCTTCAAATAAAATTAAGAAATAGTCTTTATAAGCTTGACTTGTTCAATATCTATCATCATAACCTTCATTTACTCCCCAAGGTTCATAACTCATAGGATCATCTTACACCATTAGACATGTCTGGTTTAATTTTTTTGCACTTGATCCATTCCTATTCGAAATCAATTTGCATGAAATTAAATTCTAAGATGATAGGAACCAGTTAATAATTTGATGGTCCCTGAGCATTGATGGACAGAAGATATGAGTTTATGGGTTCAAGCTTTATGAGCTTGACCTGTTCAATATCTACCATCATAACCTTCATTTACTCCCTGAGGTTTATAATTCATAGGAATAGCTCACAAGACATGTCTAGGTTAACTTTTCTGCACTTGACTATTGCTATTCAAAACTAATTCTTATTATCTGGATAGAAGGTGGAAGTAGAATCTCTATCCAGACAAGAGAAAGTAGAATTTGTTTGAAAAGCTTTTGCTTCTCTGTCAGAGATTAAACCAGGTTAGGAATCACTACCGTACAATTATATGATTTTGTCCACTTTGAGCATAAGCTCTCATGGTTTTGCCTTGGACGTCTCCATATGGTCTCATACCAATAGAGATATATTTCTTACTTATAAACCCATGATCAGTCCCTAAATTAGCCAACGTGGGACTCCCTCCAAACAATCCTCAACAAACCATTCCGTTGGGGTCAAAGCCTCAAGAAGAAACTCAGAATCTTATGAACAAATGGTGTATGGAGAGGCACTAACATGAACCATAAAGTTATCTACTCTTATCTTGAGGTGGAGGTTCGAACCCATATTTCCACGTTTATCGTACTCGAAAGTGGTAAGAAATGGAAGGCATTATTGCTGGTTTAGGACTTGGAAGGAGATATGGGAAGTGGCAAGGTGGCGTCCTTTTTATACTTTTTGAGTCGTTATATGATACTTTAAGGATGAAGTTGGCTCCATCAGAAATTAGTATTTGTTAGTTAATGTTGTACTTTTAAATCAAAGGGCTATTCTCATGCTGCTTTTATTACACCCTGGGCAGATTCGAAAGATGGTAGGCACAGCCATTGCCGTGAAGCGGAAACTGCTCCCAAGAGATATCATAGCATTGTCACTGAGCAAGTTCTCTAGAATAGTTCTGCCCATTGCTCCAGCTGAAGTCTTAGTCCTAAGAGGGAACAGGTTTTCCAACAGAAAACGACCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTAAACGAATCCCACGACATTGTCAAGTCAGTGAATGAATTCTACAGATCGGCGATATTACCCGAGGTAACTAACTTTCTAGACATAGAAAGACCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGAGGAAGTTAGGAATGCTTGGAAGGCATGGACAGGAAAAGGTAGATTGTCTTGA

mRNA sequence

GGATGCCATGGCAGTCTCTTCTCTGCGATTCCCGCTTTCCCCATGGGCAAACAAAACTTCATTTCCATGCTTCCGTTTTCCCTACCCTAATTTCGTTTCGTGGACCTCTCGTCTGAGTCCTAGACTGCTGTGCTGTTCCTCATCTTCTACTGCTTCCACCGATGCTCTTTCCACTCAGTGCGCCCCAGACCAGCTCCGGAAATGGGAAGCTTTTCGGAAGAAGAAGGTCGTTATGCGGATTGGCTATGTTGGGACTGATTATAGAGGGCTGCAAATGCAACGCGAGCAATCATCACATCCAACAATTGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAGATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGATAGTGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATCAACTTTTATCTTCCTGAAAATATCAGGGTTTTTGGCATTGTACCTTCACAGAGGAGCTTTGATCCTAGAAGGGAGTGTGATGTTCGGAAGTATACTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAGCTCACAACTACTGAAATTGATTATCACATCTCAGAATTTAAGGATATATTAAATTCGTTTCAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGGATTCGAACCAACCAATCTCCAGGATATGTCCACAAGTCAGGAGATGGGGTATCATCTAGTGATTTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAAACGATATTTTTGTTGGAGGAATCAAGGAACCAAATCAACATTCTCCAAGCTTAAGTGGTAGTTATGAGAATACATTTGAAGTATGCAGTAAACTTGGAAATGGATCAGAGCTTCCACAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAAATCCTATACTGTTCTTGTGGGAAGCTGGAAACATCTCTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCTTTCATGTTGCATCAAATTCGAAAGATGGTAGGCACAGCCATTGCCGTGAAGCGGAAACTGCTCCCAAGAGATATCATAGCATTGTCACTGAGCAAGTTCTCTAGAATAGTTCTGCCCATTGCTCCAGCTGAAGTCTTAGTCCTAAGAGGGAACAGGTTTTCCAACAGAAAACGACCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTAAACGAATCCCACGACATTGTCAAGTCAGTGAATGAATTCTACAGATCGGCGATATTACCCGAGGTAACTAACTTTCTAGACATAGAAAGACCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGAGGAAGTTAGGAATGCTTGGAAGGCATGGACAGGAAAAGGTAGATTGTCTTGA

Coding sequence (CDS)

ATGGCAGTCTCTTCTCTGCGATTCCCGCTTTCCCCATGGGCAAACAAAACTTCATTTCCATGCTTCCGTTTTCCCTACCCTAATTTCGTTTCGTGGACCTCTCGTCTGAGTCCTAGACTGCTGTGCTGTTCCTCATCTTCTACTGCTTCCACCGATGCTCTTTCCACTCAGTGCGCCCCAGACCAGCTCCGGAAATGGGAAGCTTTTCGGAAGAAGAAGGTCGTTATGCGGATTGGCTATGTTGGGACTGATTATAGAGGGCTGCAAATGCAACGCGAGCAATCATCACATCCAACAATTGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAGATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGATAGTGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATCAACTTTTATCTTCCTGAAAATATCAGGGTTTTTGGCATTGTACCTTCACAGAGGAGCTTTGATCCTAGAAGGGAGTGTGATGTTCGGAAGTATACTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAGCTCACAACTACTGAAATTGATTATCACATCTCAGAATTTAAGGATATATTAAATTCGTTTCAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGGATTCGAACCAACCAATCTCCAGGATATGTCCACAAGTCAGGAGATGGGGTATCATCTAGTGATTTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAAACGATATTTTTGTTGGAGGAATCAAGGAACCAAATCAACATTCTCCAAGCTTAAGTGGTAGTTATGAGAATACATTTGAAGTATGCAGTAAACTTGGAAATGGATCAGAGCTTCCACAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAAATCCTATACTGTTCTTGTGGGAAGCTGGAAACATCTCTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCTTTCATGTTGCATCAAATTCGAAAGATGGTAGGCACAGCCATTGCCGTGAAGCGGAAACTGCTCCCAAGAGATATCATAGCATTGTCACTGAGCAAGTTCTCTAGAATAGTTCTGCCCATTGCTCCAGCTGAAGTCTTAGTCCTAAGAGGGAACAGGTTTTCCAACAGAAAACGACCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTAAACGAATCCCACGACATTGTCAAGTCAGTGAATGAATTCTACAGATCGGCGATATTACCCGAGGTAACTAACTTTCTAGACATAGAAAGACCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGAGGAAGTTAGGAATGCTTGGAAGGCATGGACAGGAAAAGGTAGATTGTCTTGA

Protein sequence

MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAPDQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSPSLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS

Homology

[Close]

BLAST of CmoCh13G000110 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.1e-143
Identity = 268/491 (54.58%), Postives = 353/491 (71.89%), Query Frame = 0


Query: 23  RFPYPNFVSW----TSRLSPRLLCCSSSST--ASTDALSTQCAPDQLR-KWEAFRKKKVV 82
           R P P+ V W      R++P LL  SSS+    S+ +LS   + + L  KWE++RKKKVV
Sbjct: 7   RIPIPSRVFWYPTRLIRVTPALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVV 66

Query: 83  MRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARSSRTDK 142
           +RIGYVGTDYRGLQ+QR+  S  TIE ELE AIYKAGGIR+SNYGDL+KI WARSSRTDK
Sbjct: 67  IRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDK 126

Query: 143 GVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECD 202
           GVHSLAT+I+LKMEIP++AW  DP G +LA  I+ +LPENIRVF ++PS R FDPRREC 
Sbjct: 127 GVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECT 186

Query: 203 VRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRI--R 262
           +RKY+YLLP  ++GI++  T+ EIDYHI++F +IL  F+GE+PFHNYT RS+YR +   +
Sbjct: 187 LRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK 246

Query: 263 TNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSPSLSGSYENTFEVC 322
             Q  G   +    + +S+   SEF E   EN I +G  +E  +    + G  E+   V 
Sbjct: 247 IKQRNGRPPREPKSIKASE---SEFRE---ENHIEIGEEEEEKE----VDG--ESDEHVV 306

Query: 323 SKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYDFVEISIWGES 382
           +   + S++    RA+WL+EPDE D+I  +HFRK+  C  GKLE SLG+ FVEISIWGES
Sbjct: 307 TPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEISIWGES 366

Query: 383 FMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVLRGNRFSNRKRPG 442
           FMLHQIRKM+GTA+AVKR+LLPRDII LSL+KF+RIVLP+AP+EVL+LRGN F  R+   
Sbjct: 367 FMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRR--- 426

Query: 443 APKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQ 502
            P+RP +    ES ++ K + EFYR+ ++P+V+ FLD E+ PWKEWV+ LD+   + +E+
Sbjct: 427 LPERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEE 480

Query: 503 LEEVRNAWKAW 505
           LE+VR  W+ W
Sbjct: 487 LEDVRKGWEEW 480

[Close]

BLAST of CmoCh13G000110 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 4.5e-25
Identity = 110/370 (29.73%), Postives = 172/370 (46.49%), Query Frame = 0


Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARS 130
           K+KV + +GY GT Y G+Q+Q + +   TIE+++  A+  AG I   N  DL K  + R+
Sbjct: 148 KRKVAVMLGYCGTGYNGMQVQNDPNV-KTIEKDIYDAMATAGAISAENAVDLKKSGFQRA 207

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LKM I D           + N IN  LP+ IR++GI  + + FD 
Sbjct: 208 ARTDKGVHAAGNVISLKMIIEDPE---------IINKINDLLPKQIRIWGIQRTTKGFDC 267

Query: 191 RRECDVRKYTYLLPA-KLIGIESKLTTTEIDYHISEFK-DILNSFQGEHPFHNY--TIRS 250
           R+ C  R Y YLLP   L+  + K   +E+   + E K +  + F+ +    ++   ++S
Sbjct: 268 RKCCSSRIYEYLLPTFSLLPPKPKSVLSEL---VKEKKLENPDLFEDDQEGIDWWENVKS 327

Query: 251 KYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIF----------VGGIKEPN 310
           K      T +    +  S D          + +  + E DI           +  I+  +
Sbjct: 328 KILASGITQEQIDSITSSYDEQQQQQQQQQQQQADEEERDISELSFTKLIKQIKTIENQS 387

Query: 311 QHSPSLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKL 370
           + S  +S S    F    K   G+          + +P  KD        K +      +
Sbjct: 388 RRSYRISSSRLQHFREVMKQYEGT---HNFHNFTVGKP-FKDTSANRFMIKTIVSDPFVI 447

Query: 371 ETSLGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPA 427
           E   G +++ I I G+SFMLHQIRKM+  A  V R  LP  II  +  + ++I +P APA
Sbjct: 448 E---GTEWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIIN-NFFQSTKINIPKAPA 496

[Close]

BLAST of CmoCh13G000110 vs. ExPASy Swiss-Prot
Match: Q6FV05 (tRNA pseudouridine synthase 1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.8e-24
Identity = 120/438 (27.40%), Postives = 191/438 (43.61%), Query Frame = 0


Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARS 130
           K+KV + IGY GT Y G+Q       +PTIE  L  A   AG I ++N  DL K ++ R+
Sbjct: 86  KRKVAVMIGYCGTGYHGMQY---NPPNPTIEAALFKAFVDAGAISKANSNDLKKNNFMRA 145

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LKM I D   M         + IN  LPE IR++ I    ++FD 
Sbjct: 146 ARTDKGVHAGGNLISLKMIIEDPEIM---------DKINANLPEGIRIWDIERVNKAFDC 205

Query: 191 RRECDVRKYTYLLPA-KLIGIE-SKLTTTEIDYHISEFKDILN-SFQGEHPFHNYTIRSK 250
           R+ C  R Y YLLP   LIG +   +   +I+   +E   +L+   + +  + N+   +K
Sbjct: 206 RKMCSSRWYEYLLPTYSLIGPKPGTILNNDIESSKTELPGVLDEDLESKEFWENFEAAAK 265

Query: 251 YRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSPSLSGSYE 310
                    +P  +       +  D S    EE D + +++     +  +    L  ++ 
Sbjct: 266 ------KEFTPEEI------AAIKDYSPPPREEFDDQEELY-----QKVKQWKLLENTHR 325

Query: 311 NTFEVCSKLGN--GSELPQGVRARWLHE----PDEKDRIGASHFRKILYCSCGKLETSLG 370
             + +     N   + + Q + A   H      + KD   A  F K +  S   +     
Sbjct: 326 RQYRISEMKLNKFRAAMNQYLGAHNFHNFTLGKEFKDP-SAIRFMKEIKVSDPFVIGDAK 385

Query: 371 YDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVL 430
            ++V I I G+SFMLHQIRKM+  A  + R   P + I+ +     +I +P APA  L+L
Sbjct: 386 AEWVSIKIHGQSFMLHQIRKMISMATLIARCGTPVERISQAFGP-QKINIPKAPALGLLL 445

Query: 431 RG---NRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKE 490
                  ++ R      K  D     E  D  K +   Y      EV++ +      +  
Sbjct: 446 EAPVFESYNKRLEKFGYKPIDFSKYQEKVDAFK-MKHIYDKIYAEEVSDNV------FNA 485

Query: 491 WVEKLDKYTSIPNEQLEE 497
           +   +D +  +   Q EE
Sbjct: 506 FFSYIDSFNKVTGAQTEE 485

[Close]

BLAST of CmoCh13G000110 vs. ExPASy Swiss-Prot
Match: Q6CWQ8 (tRNA pseudouridine synthase 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 3.2e-23
Identity = 110/359 (30.64%), Postives = 161/359 (44.85%), Query Frame = 0


Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARS 130
           K+KV + IGY GT Y G+Q       + TIE+EL  A  +AG I ++N  DL K  + R+
Sbjct: 98  KRKVAVMIGYCGTGYHGMQY---NPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRA 157

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LK+ I D           +   IN  LP+ IRV+ I    ++FD 
Sbjct: 158 ARTDKGVHAGGNVISLKLIIEDPE---------VKEKINNELPDQIRVWDISRVNKAFDC 217

Query: 191 RRECDVRKYTYLLPA-KLIGIE-SKLTTTEIDYHISEFKDILNSFQGEHPF---HNYTIR 250
           R+ C  R Y YLLP   LIG + S     EI+    E  +++        F    +    
Sbjct: 218 RKMCSSRWYEYLLPTYSLIGPKPSTYLYNEIEASKKEIPNVIQDDVESAQFWEAFSTEAE 277

Query: 251 SKYRTRIRTNQSPGYV--HKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSPSLS 310
           SK+ T+    +   +V    S D  + +   +  F++ + E+       KE      S  
Sbjct: 278 SKF-TKEELEEIAAFVMPKDSFDENNETYQKSKAFKKLEAEHKRAYRISKERLDRFRSAL 337

Query: 311 GSYENTFEVCS-KLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGY 370
             YE TF   +  LG     P                  A  F K +  S   +      
Sbjct: 338 KQYEGTFNFHNFTLGKDFNDP-----------------SAKRFMKQITVSEPFVIGEAKT 397

Query: 371 DFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVL 422
           ++V I I G+SFMLHQIRKM+  A  + R   P + I  +  +  +I +P APA  L+L
Sbjct: 398 EWVSIKIHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQ-EKINIPKAPALGLLL 425

[Close]

BLAST of CmoCh13G000110 vs. ExPASy Swiss-Prot
Match: Q12211 (tRNA pseudouridine synthase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PUS1 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.7e-22
Identity = 108/385 (28.05%), Postives = 157/385 (40.78%), Query Frame = 0


Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARS 130
           K+KV + +GY GT Y G+Q       +PTIE  L  A  +AG I + N  DL K  + R+
Sbjct: 74  KRKVAVMVGYCGTGYHGMQY---NPPNPTIESALFKAFVEAGAISKDNSNDLKKNGFMRA 133

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LKM I D           +   IN  LPE IRV+ I    ++FD 
Sbjct: 134 ARTDKGVHAGGNLISLKMIIEDPD---------IKQKINEKLPEGIRVWDIERVNKAFDC 193

Query: 191 RRECDVRKYTYLLPA-KLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYR 250
           R+ C  R Y YLLP   LIG +              ++DI  S                +
Sbjct: 194 RKMCSSRWYEYLLPTYSLIGPKPGSIL---------YRDIEES----------------K 253

Query: 251 TRIRTNQSPGYVHKSGDGVSSSDLSNSEFEE---SDGENDIFVGGIKEPNQHSPSLSGSY 310
           T +              GV   DL + EF E    D         I+    + P     +
Sbjct: 254 TEL-------------PGVLDEDLESKEFWEEFKKDANEKFSTEEIEAILAYVPPARDEF 313

Query: 311 ENTFEVCSKLGNGSELPQGVRARW--------LHEPDEKDRIGASHFRKILYCSCGKLET 370
           +   E+  K+    +L    R R+                 +GA +F         K  +
Sbjct: 314 DINEELYQKVKKYKQLENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKEPS 373

Query: 371 SLGY-----------------DFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIAL 427
           ++ +                 +++ I I G+SFMLHQIRKMV  A  + R   P + I+ 
Sbjct: 374 AIRFMKDIKVSDPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERISQ 407

[Close]

BLAST of CmoCh13G000110 vs. ExPASy TrEMBL
Match: A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.7e-301
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
           NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512
           EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511

[Close]

BLAST of CmoCh13G000110 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 8.5e-293
Identity = 500/512 (97.66%), Postives = 504/512 (98.44%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWA KTSFPCFRFPYPNFVSWTSRL PRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENI+VFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFV-GGIKEPNQHS 300
           NYTIRSKYRTRIR NQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFV GGIKEPNQHS
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PSLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 360
           PSLSG  ENTFEVCSKLGNGSELPQGVRARWLHEPDE+DRIGASHFRKIL CSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420
           LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL
Sbjct: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420

Query: 421 VLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480
           VLRGNRFSNRKRPGAPKRPDLLTLN+S DIVKSVNEFYRSAILPEVTNFLDIERPPWKEW
Sbjct: 421 VLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480

Query: 481 VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512
           VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512

[Close]

BLAST of CmoCh13G000110 vs. ExPASy TrEMBL
Match: A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.9e-248
Identity = 428/507 (84.42%), Postives = 457/507 (90.14%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPW  KTS  CFR PY  FVS +  L PRLLCC SS +ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLR WEAFRKKKVVMRIGY GTDYRGLQMQREQ SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +L KISWARSSRTDKGVHSLATTI+LKMEIP++AWMGDPCGILLANYINFYLP+NI+VFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKY+YLLPAKLIGIESK  TTEID+HISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
           NYTIRSKYR  IR  QSPGY H SG+ VS S +SNS FEESD E++ FV G+K PNQH+P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300

Query: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
            LS   E+  EVCSKLGNGSEL +GVRARWLHEPDEKDRIGASHFRKIL CSCGKLETSL
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGN FSN KRPGAPKRPDLLTLN+S DI+KSV+EFYRSAILPE+TNFLD E+ PW EWV
Sbjct: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGK 508
           EKLDKYTSIPNEQL+EVRNAWKAW  K
Sbjct: 481 EKLDKYTSIPNEQLDEVRNAWKAWKEK 506

[Close]

BLAST of CmoCh13G000110 vs. ExPASy TrEMBL
Match: A0A6J1DQ07 (putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.9e-248
Identity = 428/507 (84.42%), Postives = 457/507 (90.14%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPW  KTS  CFR PY  FVS +  L PRLLCC SS +ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLR WEAFRKKKVVMRIGY GTDYRGLQMQREQ SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +L KISWARSSRTDKGVHSLATTI+LKMEIP++AWMGDPCGILLANYINFYLP+NI+VFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKY+YLLPAKLIGIESK  TTEID+HISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
           NYTIRSKYR  IR  QSPGY H SG+ VS S +SNS FEESD E++ FV G+K PNQH+P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300

Query: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
            LS   E+  EVCSKLGNGSEL +GVRARWLHEPDEKDRIGASHFRKIL CSCGKLETSL
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGN FSN KRPGAPKRPDLLTLN+S DI+KSV+EFYRSAILPE+TNFLD E+ PW EWV
Sbjct: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGK 508
           EKLDKYTSIPNEQL+EVRNAWKAW  K
Sbjct: 481 EKLDKYTSIPNEQLDEVRNAWKAWKEK 506

[Close]

BLAST of CmoCh13G000110 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.3e-244
Identity = 420/504 (83.33%), Postives = 453/504 (89.88%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           M+VSSLR PLSPW  K+S  CFRFPYP FV  T RL P LL C SS+ ASTDA STQCAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKW+AFRKKKVV+RIGYVGTDYRGLQMQR Q SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LA+YIN YLP+NI+VFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTEID HIS+FKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
           NYTIRS YRTRIR  QSPGY  KSG+ VS  ++SN EFEESD E+D FV G+KEP+Q SP
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300

Query: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLS   EN  EVCSKL +GSEL +GVRARWLHEPDEKDRIGASHFR+IL CSCGKLETS 
Sbjct: 301 SLS-VCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKM+GTA+AVKRKLLPRD+IALSL+KFSRIVLPIAPAEVLV
Sbjct: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDVIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGN FS RKRPG P RPDL+TLNES DI+KSVNEFYRSAILPEVT FLD E PPWKEWV
Sbjct: 421 LRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPEVTEFLDTESPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAW 505
           EKLDKYTSIPNE+L+EVR+AWKAW
Sbjct: 481 EKLDKYTSIPNEELDEVRSAWKAW 503

[Close]

BLAST of CmoCh13G000110 vs. NCBI nr
Match: XP_022964935.1 (putative tRNA pseudouridine synthase [Cucurbita moschata])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.5e-301
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
           NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512
           EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511

[Close]

BLAST of CmoCh13G000110 vs. NCBI nr
Match: KAG6583326.1 (putative tRNA pseudouridine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1030.4 bits (2663), Expect = 5.3e-297
Identity = 505/511 (98.83%), Postives = 507/511 (99.22%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWA KTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
           NYTIRSKYRTRIR NQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIF GGIKEPNQHSP
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300

Query: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLSGSYENTFEV SKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL
Sbjct: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIV+PIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512
           EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511

[Close]

BLAST of CmoCh13G000110 vs. NCBI nr
Match: XP_023520301.1 (putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.2e-295
Identity = 500/511 (97.85%), Postives = 504/511 (98.63%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWA KTSFPCFRFPYPNFV WTSRL PRLLCCSSSSTASTDALSTQCAP
Sbjct: 18  MAVSSLRFPLSPWATKTSFPCFRFPYPNFVLWTSRLRPRLLCCSSSSTASTDALSTQCAP 77

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 78  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 137

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENI+VFG
Sbjct: 138 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 197

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 198 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 257

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
           NYTIRSKYRTRIR NQSPGYVHKSGDGVSSSD SNSEFEESDGENDIFVGGIKEPNQHSP
Sbjct: 258 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDFSNSEFEESDGENDIFVGGIKEPNQHSP 317

Query: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGA HFRKILYCSCGKLETS 
Sbjct: 318 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGAPHFRKILYCSCGKLETSR 377

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSL+KFSRIV+PIAPAEVLV
Sbjct: 378 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLTKFSRIVMPIAPAEVLV 437

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV
Sbjct: 438 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 497

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512
           EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 498 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 528

[Close]

BLAST of CmoCh13G000110 vs. NCBI nr
Match: XP_022970646.1 (putative tRNA pseudouridine synthase [Cucurbita maxima])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.7e-292
Identity = 500/512 (97.66%), Postives = 504/512 (98.44%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWA KTSFPCFRFPYPNFVSWTSRL PRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENI+VFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFV-GGIKEPNQHS 300
           NYTIRSKYRTRIR NQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFV GGIKEPNQHS
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PSLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 360
           PSLSG  ENTFEVCSKLGNGSELPQGVRARWLHEPDE+DRIGASHFRKIL CSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420
           LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL
Sbjct: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420

Query: 421 VLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480
           VLRGNRFSNRKRPGAPKRPDLLTLN+S DIVKSVNEFYRSAILPEVTNFLDIERPPWKEW
Sbjct: 421 VLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480

Query: 481 VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512
           VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512

[Close]

BLAST of CmoCh13G000110 vs. NCBI nr
Match: XP_038894774.1 (putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 890.2 bits (2299), Expect = 8.5e-255
Identity = 437/504 (86.71%), Postives = 463/504 (91.87%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLR PLSPW  KTS  CF FPYP FVS T RL P L  CS+SSTASTDA STQCAP
Sbjct: 1   MAVSSLRLPLSPWITKTSSSCFLFPYPTFVSRTHRLGPTLSGCSASSTASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           +QLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  NQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +LNKISWARSSRTDKGVHSLATTI LKMEIP+SAWMGDPCGI LANYINFYLP+NI+VFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTIALKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQ+SFDPR+ECDVRKY+Y+LPAKLIGI +KL TTE+DYHIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQKSFDPRKECDVRKYSYVLPAKLIGIGNKLKTTEVDYHISDFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
           NYTIRSKYRTRIR  +SPGY H+    VS SD+SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 241 NYTIRSKYRTRIRAKRSPGYGHE----VSPSDVSNSEFEESDGEDDTFVEGVKEPDQGSP 300

Query: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLS  YEN  EVCSKLG+GSELP+ VRARWLHEPDEKDRIGASHFRKIL CSCGKLETS 
Sbjct: 301 SLSVCYENPTEVCSKLGDGSELPKDVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKM+GTAIAVKRKLLPRD+IALSLSKFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMIGTAIAVKRKLLPRDVIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGN FSNRKRPG PKRPDLLTLNES DIVKSVNEFYRSAILPEVTNFLD ERPPWKEWV
Sbjct: 421 LRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSAILPEVTNFLDTERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAW 505
           EKLDKYTSIP+EQL+EVRNAWKAW
Sbjct: 481 EKLDKYTSIPSEQLDEVRNAWKAW 500

[Close]

BLAST of CmoCh13G000110 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.2e-144
Identity = 268/491 (54.58%), Postives = 353/491 (71.89%), Query Frame = 0


Query: 23  RFPYPNFVSW----TSRLSPRLLCCSSSST--ASTDALSTQCAPDQLR-KWEAFRKKKVV 82
           R P P+ V W      R++P LL  SSS+    S+ +LS   + + L  KWE++RKKKVV
Sbjct: 7   RIPIPSRVFWYPTRLIRVTPALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVV 66

Query: 83  MRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARSSRTDK 142
           +RIGYVGTDYRGLQ+QR+  S  TIE ELE AIYKAGGIR+SNYGDL+KI WARSSRTDK
Sbjct: 67  IRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDK 126

Query: 143 GVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECD 202
           GVHSLAT+I+LKMEIP++AW  DP G +LA  I+ +LPENIRVF ++PS R FDPRREC 
Sbjct: 127 GVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECT 186

Query: 203 VRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRI--R 262
           +RKY+YLLP  ++GI++  T+ EIDYHI++F +IL  F+GE+PFHNYT RS+YR +   +
Sbjct: 187 LRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK 246

Query: 263 TNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSPSLSGSYENTFEVC 322
             Q  G   +    + +S+   SEF E   EN I +G  +E  +    + G  E+   V 
Sbjct: 247 IKQRNGRPPREPKSIKASE---SEFRE---ENHIEIGEEEEEKE----VDG--ESDEHVV 306

Query: 323 SKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYDFVEISIWGES 382
           +   + S++    RA+WL+EPDE D+I  +HFRK+  C  GKLE SLG+ FVEISIWGES
Sbjct: 307 TPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEISIWGES 366

Query: 383 FMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVLRGNRFSNRKRPG 442
           FMLHQIRKM+GTA+AVKR+LLPRDII LSL+KF+RIVLP+AP+EVL+LRGN F  R+   
Sbjct: 367 FMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRR--- 426

Query: 443 APKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQ 502
            P+RP +    ES ++ K + EFYR+ ++P+V+ FLD E+ PWKEWV+ LD+   + +E+
Sbjct: 427 LPERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEE 480

Query: 503 LEEVRNAWKAW 505
           LE+VR  W+ W
Sbjct: 487 LEDVRKGWEEW 480

[Close]

BLAST of CmoCh13G000110 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-26
Identity = 110/416 (26.44%), Postives = 186/416 (44.71%), Query Frame = 0


Query: 39  RLLCCSSSSTASTDALSTQCAPDQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHP 98
           +L   ++     + A++T  +    R+   +R++KV +   + G  Y+G+Q   +     
Sbjct: 25  KLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKVAIVFAFCGVGYQGMQ---KNPGAK 84

Query: 99  TIEQELEAAIYKAGGIRESNYGDLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGD 158
           TIE ELE A++ AG + +++        WARS+RTDKGV ++   ++ +  +       D
Sbjct: 85  TIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGVSAVGQVVSGRFYV-------D 144

Query: 159 PCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECDVRKYTYLLPA----KLIGIESKL 218
           P G +    +N  LP+ IRVFG      SF  ++ CD R+Y YL+P       +  E+++
Sbjct: 145 PPGFV--ERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEM 204

Query: 219 TTTEIDYHISEFKDILNSFQG-EHPF----HNYTIRSKYRTRIRTNQSPGYVHKSGDGVS 278
             T++ Y     K +  S +G + P      +          I+++ S       G  V 
Sbjct: 205 VRTDLGYEY--VKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISSSTCDAIGADVK 264

Query: 279 SSDLSNSEFEESDGENDIFVGGIKEPNQHSPSLSGSYENTF-EVCSKLGNGSELPQGVRA 338
              LS+S     +  N   + G       S +      NT  +V    G G ++ +   +
Sbjct: 265 CETLSSSVPSAENNLNSEVLDGADVSALSSKAEGMEESNTLAKVEMNNGEGEDMTKS-ES 324

Query: 339 RWLHEPDEKDR--------IGASHF---------------RKILYCSCGKLETSLGYDFV 398
           ++ +   EK+R        +G+ +F               R IL  +   +    G DFV
Sbjct: 325 KFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANRYILSFNANTVINLDGKDFV 384

Query: 399 EISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVL 422
           +  + G+SFMLHQIRKM+G A+A+ R   P  +I  S  K  RI +P+AP   L L
Sbjct: 385 KCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMAPEVGLYL 425

[Close]

BLAST of CmoCh13G000110 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-24
Identity = 109/395 (27.59%), Postives = 174/395 (44.05%), Query Frame = 0


Query: 72  KKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARSS 131
           +KV +   + G  Y+G+Q   +     TIE ELE A++ AG + ES  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 132 RTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPR 191
           RTDKGV ++        ++    ++ DP G +  N +N  LP  IR+FG      SF  +
Sbjct: 82  RTDKGVSAVG-------QVVSGRFIVDPLGFV--NRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 192 RECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTR 251
           + CD R+Y YLLP   +   S      +   +   ++ +  F+           S+   +
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFE----------CSERGRK 201

Query: 252 IRTNQSPGYVHK---SGDGVSSSDLSN--SEFEESDGENDIFVGGIKEPNQHSPSLSGSY 311
           I     PG V K   +  G  S D  +  S    S   +DI +  +      S +L+G  
Sbjct: 202 I----PPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEAL------SSNLAGLC 261

Query: 312 ENTFEV-------CSKLGNGSELPQGVRARWLHEPDEKDR--------IGASHF------ 371
               EV       C    N SE    V++++ +   EK+R        +G+ +F      
Sbjct: 262 SVDVEVGRIQEDSCKLNTNSSETK--VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTR 321

Query: 372 ---------RKILYCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPR 431
                    R+I+  +   +    G DF++  + G+SFMLHQIRKM+G A+A+ R     
Sbjct: 322 TKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASE 381

[Close]

BLAST of CmoCh13G000110 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 104.8 bits (260), Expect = 2.2e-22
Identity = 101/369 (27.37%), Postives = 162/369 (43.90%), Query Frame = 0


Query: 72  KKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARSS 131
           +KV +   + G  Y+G+Q   +     TIE ELE A++ AG + ES  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 132 RTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPR 191
           RTDKGV ++        ++    ++ DP G +  N +N  LP  IR+FG      SF  +
Sbjct: 82  RTDKGVSAVG-------QVVSGRFIVDPLGFV--NRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 192 RECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTR 251
           + CD R+Y YLLP   +   S      +   +   ++ +  F+           S+   +
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFE----------CSERGRK 201

Query: 252 IRTNQSPGYVHK---SGDGVSSSDLSN--SEFEESDGENDIFVGGIKEPNQHSPSLSGSY 311
           I     PG V K   +  G  S D  +  S    S   +DI +  +      S +L+G  
Sbjct: 202 I----PPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEAL------SSNLAGLC 261

Query: 312 ENTFEV-------CSKLGNGSELPQGVRARWLHEPDEKDR--------IGASHF------ 371
               EV       C    N SE    V++++ +   EK+R        +G+ +F      
Sbjct: 262 SVDVEVGRIQEDSCKLNTNSSETK--VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTR 321

Query: 372 ---------RKILYCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPR 406
                    R+I+  +   +    G DF++  + G+SFMLHQIRKM+G A+A+ R     
Sbjct: 322 TKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASE 356

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BLAST of CmoCh13G000110 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 53.9 bits (128), Expect = 4.4e-07
Identity = 47/183 (25.68%), Postives = 71/183 (38.80%), Query Frame = 0


Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARS 130
           K+ V ++I Y G  + G       S+   +E  +E+ ++KA        GD     ++R 
Sbjct: 89  KRYVALKIMYFGKRFYGF------SAEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRC 148

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGIL-----------LANYINFYLPENIRVF 190
            RTDKGV S    I L +     +  GD    +               +N  LP++IRV 
Sbjct: 149 GRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVI 208

Query: 191 GIVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPF 243
           G  P+   F  R  C  R+Y Y            L  + +D+           F GEH F
Sbjct: 209 GWSPAPIDFHARFSCYAREYKYFF------WRQNLNLSAMDF-------AGKKFIGEHDF 252

The following BLAST results are available for this feature:

BLAST of CmoCh13G000110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22928	3.1e-143	54.58	Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032...	[more]
Q59S63	4.5e-25	29.73	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q6FV05	3.8e-24	27.40	tRNA pseudouridine synthase 1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / ...	[more]
Q6CWQ8	3.2e-23	30.64	tRNA pseudouridine synthase 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 23...	[more]
Q12211	2.7e-22	28.05	tRNA pseudouridine synthase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / ...	[more]

BLAST of CmoCh13G000110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HM83	1.7e-301	100.00	putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648...	[more]
A0A6J1I156	8.5e-293	97.66	putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569...	[more]
A0A6J1DMV6	2.9e-248	84.42	putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DQ07	2.9e-248	84.42	putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A1S3C5W1	4.3e-244	83.33	putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=...	[more]

BLAST of CmoCh13G000110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022964935.1	3.5e-301	100.00	putative tRNA pseudouridine synthase [Cucurbita moschata]	[more]
KAG6583326.1	5.3e-297	98.83	putative tRNA pseudouridine synthase, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_023520301.1	2.2e-295	97.85	putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo]	[more]
XP_022970646.1	1.7e-292	97.66	putative tRNA pseudouridine synthase [Cucurbita maxima]	[more]
XP_038894774.1	8.5e-255	86.71	putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida]	[more]

BLAST of CmoCh13G000110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30320.1	2.2e-144	54.58	Pseudouridine synthase family protein	[more]
AT1G20370.1	1.4e-26	26.44	Pseudouridine synthase family protein	[more]
AT1G76120.1	1.0e-24	27.59	Pseudouridine synthase family protein	[more]
AT1G76120.2	2.2e-22	27.37	Pseudouridine synthase family protein	[more]
AT1G34150.1	4.4e-07	25.68	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 195..496 e-value: 1.9E-34 score: 120.9
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 341..422 e-value: 5.6E-7 score: 30.0
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 63..194 e-value: 1.9E-31 score: 110.6
None	No IPR available	PIRSR	PIRSR001430-2	PIRSR001430-2	coord: 124..254 e-value: 9.6E-16 score: 55.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 255..276
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 255..301
None	No IPR available	PANTHER	PTHR11142:SF21	TRNA PSEUDOURIDINE SYNTHASE	coord: 41..505
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 41..505
IPR041708	Pseudouridine synthase PUS1/ PUS2-like	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 76..426 e-value: 7.18308E-80 score: 247.527
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 342..428
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 71..245

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh13G000110.1	CmoCh13G000110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding