CmoCh10G000500.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh10G000500.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmediator of RNA polymerase II transcription subunit 30
LocationCmo_Chr10: 240045 .. 241890 (-)
Sequence length1042
RNA-Seq ExpressionCmoCh10G000500.1
SyntenyCmoCh10G000500.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGATTATTTTTAATTTCATTTGTTTTATTTATTTATTTTTTTTTTTGGCTTCCTCGATTTCAATTTACCGATGAGGGTTAGGGTTTCATGTGAACATCAGAAGTGTGATTGAAAGTCGGAATTGGGATTTTGGTTGATTCCGAAGGCTCTCTCTACGAATTCGGTGGATTTTCGGTTGGCGTAGCAGCACAAAAGCTTCTGTTTTTTGTTCTGGAAAAATTTGATGGAAGAGAAACCCACTAATGTTTTGTCTCTTCCCCAGACAACTCAAGAGCTTGCAATGGAGGGCATCAAGCATCTTGAGGAAACGATAGAATCCGCATTCCAGATCCTATACTCCATGAATGACGAGCTCTGCAATCCCACATTATGGTCCACCACTTCTTCAACGGCAACAACGACTACTTCTGGTTTAACAATTGCCAGCCCAAATGCTCCATCTTCGCATTCCACTAATGGAGTTGTAAACGGCGATGCAAGTTCGGAAAGCACAAACCATCACACCGACACCAGCAGTGGCGGAGGCAGCGGTAGTGGGGGAGCCCTTGAGGAGGCCCGTGTTCGCTACAAGAATTCAGTACTTGCACTTCGTGCTATTCTTGCTGCAATTCCCAATTCTCAGAAGGTAAATTACTGACTGATCTTCCTCTTATTGGACTTTGTTCATCAGCCAGCGTTTCTCATTTCTCATTTGGCAAAATTCGAGATTGATTCGGAGATATTTGGTTCCCCCATGTTACAAAATGCTTTGTGGAGAACATGGAATTTTTGTATTCAATTTCCGCCGAGTGCCTAAAATTCTTCCTGAATTTGATAGATTGCAGATACTACATTCGACCTGGTTTTCATTTTCACCACTGCTGATAGATGCGTGGACTTAAGCCATGTCTATGAATTTGATAGATTGCTAACTGCATTGACTTGTTGTTCACTTATGCCGATTAAGCGACTTAAATCAGAGTTTCATGGTTTATTGATAGGCAAAAGCACATGAATCGGGTACAAGCGGTCATGCATCGCCTGCAGACCAATCTGAAATTGATAAGTTGGAACAACACCTCTCTAATCTCAGAAAGGTATTCTATTGTATCTTCAGCTTGTAAATTCATGTCAGTAATTTATTTTTTCTTCAAGACTCTTAGGATTACAACCAGTTAGAATTTTGTGGCAAAGATTTCATCTGGGAACCAAATATTTCTGGAAATAATTTACTTATATAAGACTTTGGAGGTAAATATATAGTAAAATTTACTAAAATATGTACAAATATTGATGAAACTGAGAAGGTCTTTAAAGATTTCAAGACAACAGTTCTGTTTGTCTTATTGTTTGTTGTATATCAGAACTAATCTAAGGAAAGATTTTGAAACTAAATAGGCAATTGAGAAAATCAAATAGTGAAACTAATAACGTTATGGAGGTTCTGTCATGGATATTATAGGTAATATGCTTCTTCCTAGAAATGACATCTTCTTTTTCTTTCAACTGCTATCTTACAAGGGTCCTATCTGCGGTGTGTTTGCAGGAACTTATGGTTAAGAACTTGCATATAAAGCTTCTGATAGATCAACTACGAGAACTTGTTGCTGATGTATCTACATGGCAAAGTCCTTGTTCTGTATGAAAAACATGACTAGCGTTGAGTCTCCTCACTAATCTAGCTGTCGTAATTTGAGAATGTCTTGCTCTGTTTCTTTATAAGATGAGCTGTTATTAGAACGTTTCTTTATATATCTCTTCAATGAAACTGGGATTATAAATCTGCCATTGGCACTAGATTGTTAATATAACTTAAGGCTCTGATAAATCAAGTACCGGGAATAAAAGGTTCAATATTTCCATGAA

mRNA sequence

ACGATTATTTTTAATTTCATTTGTTTTATTTATTTATTTTTTTTTTTGGCTTCCTCGATTTCAATTTACCGATGAGGGTTAGGGTTTCATGTGAACATCAGAAGTGTGATTGAAAGTCGGAATTGGGATTTTGGTTGATTCCGAAGGCTCTCTCTACGAATTCGGTGGATTTTCGGTTGGCGTAGCAGCACAAAAGCTTCTGTTTTTTGTTCTGGAAAAATTTGATGGAAGAGAAACCCACTAATGTTTTGTCTCTTCCCCAGACAACTCAAGAGCTTGCAATGGAGGGCATCAAGCATCTTGAGGAAACGATAGAATCCGCATTCCAGATCCTATACTCCATGAATGACGAGCTCTGCAATCCCACATTATGGTCCACCACTTCTTCAACGGCAACAACGACTACTTCTGGTTTAACAATTGCCAGCCCAAATGCTCCATCTTCGCATTCCACTAATGGAGTTGTAAACGGCGATGCAAGTTCGGAAAGCACAAACCATCACACCGACACCAGCAGTGGCGGAGGCAGCGGTAGTGGGGGAGCCCTTGAGGAGGCCCGTGTTCGCTACAAGAATTCAGTACTTGCACTTCGTGCTATTCTTGCTGCAATTCCCAATTCTCAGAAGGCAAAAGCACATGAATCGGGTACAAGCGGTCATGCATCGCCTGCAGACCAATCTGAAATTGATAAGTTGGAACAACACCTCTCTAATCTCAGAAAGGAACTTATGGTTAAGAACTTGCATATAAAGCTTCTGATAGATCAACTACGAGAACTTGTTGCTGATGTATCTACATGGCAAAGTCCTTGTTCTGTATGAAAAACATGACTAGCGTTGAGTCTCCTCACTAATCTAGCTGTCGTAATTTGAGAATGTCTTGCTCTGTTTCTTTATAAGATGAGCTGTTATTAGAACGTTTCTTTATATATCTCTTCAATGAAACTGGGATTATAAATCTGCCATTGGCACTAGATTGTTAATATAACTTAAGGCTCTGATAAATCAAGTACCGGGAATAAAAGGTTCAATATTTCCATGAA

Coding sequence (CDS)

ATGGAAGAGAAACCCACTAATGTTTTGTCTCTTCCCCAGACAACTCAAGAGCTTGCAATGGAGGGCATCAAGCATCTTGAGGAAACGATAGAATCCGCATTCCAGATCCTATACTCCATGAATGACGAGCTCTGCAATCCCACATTATGGTCCACCACTTCTTCAACGGCAACAACGACTACTTCTGGTTTAACAATTGCCAGCCCAAATGCTCCATCTTCGCATTCCACTAATGGAGTTGTAAACGGCGATGCAAGTTCGGAAAGCACAAACCATCACACCGACACCAGCAGTGGCGGAGGCAGCGGTAGTGGGGGAGCCCTTGAGGAGGCCCGTGTTCGCTACAAGAATTCAGTACTTGCACTTCGTGCTATTCTTGCTGCAATTCCCAATTCTCAGAAGGCAAAAGCACATGAATCGGGTACAAGCGGTCATGCATCGCCTGCAGACCAATCTGAAATTGATAAGTTGGAACAACACCTCTCTAATCTCAGAAAGGAACTTATGGTTAAGAACTTGCATATAAAGCTTCTGATAGATCAACTACGAGAACTTGTTGCTGATGTATCTACATGGCAAAGTCCTTGTTCTGTATGA

Protein sequence

MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTTTSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVLALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLIDQLRELVADVSTWQSPCSV
Homology
BLAST of CmoCh10G000500.1 vs. ExPASy Swiss-Prot
Match: Q9FMV4 (Mediator of RNA polymerase II transcription subunit 30 OS=Arabidopsis thaliana OX=3702 GN=MED30 PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.1e-43
Identity = 107/190 (56.32%), Postives = 139/190 (73.16%), Query Frame = 0

Query: 14  TTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWST--TSSTATTTTSGLTIASPNA 73
           TTQELAMEG K LEETIE+AFQI+ +MNDELCNP+LWST  T S+A TTT          
Sbjct: 13  TTQELAMEGEKQLEETIEAAFQIISAMNDELCNPSLWSTSATPSSAATTTGS-------- 72

Query: 74  PSSHSTNGVVNGDASS-ESTNHHTDTSSGGGSGSGG--ALEEARVRYKNSVLALRAILAA 133
               + + +V+ DA++ + T+HH++++ GGG G  G   L+EA +RYKNSV +LRA+LAA
Sbjct: 73  ----NGSALVSADAAAIDGTSHHSESAGGGGGGGSGNSVLDEASLRYKNSVTSLRAVLAA 132

Query: 134 IPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLIDQLRELVAD 193
           IPNSQKAKA E   +G  SP  + EI+KLE+   +LR E+  KN+H+K LID+LREL+AD
Sbjct: 133 IPNSQKAKASEM-QNGLGSPESEDEIEKLEEQALSLRMEIAKKNVHVKELIDKLRELIAD 189

Query: 194 VSTWQSPCSV 199
           +STWQSPCSV
Sbjct: 193 ISTWQSPCSV 189

BLAST of CmoCh10G000500.1 vs. ExPASy TrEMBL
Match: A0A6J1JFZ2 (mediator of RNA polymerase II transcription subunit 30 OS=Cucurbita maxima OX=3661 GN=LOC111484777 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.7e-98
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT
Sbjct: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLRELVADVSTWQSPCSV
Sbjct: 181 QLRELVADVSTWQSPCSV 198

BLAST of CmoCh10G000500.1 vs. ExPASy TrEMBL
Match: A0A6J1E0F5 (mediator of RNA polymerase II transcription subunit 30 OS=Cucurbita moschata OX=3662 GN=LOC111429707 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.7e-98
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT
Sbjct: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLRELVADVSTWQSPCSV
Sbjct: 181 QLRELVADVSTWQSPCSV 198

BLAST of CmoCh10G000500.1 vs. ExPASy TrEMBL
Match: A0A0A0LNW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G001500 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 8.6e-92
Identity = 186/198 (93.94%), Postives = 193/198 (97.47%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEK TNVL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATT 
Sbjct: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASP+ PSSHS NGVVNGDASSEST+HHTD+S GGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKAKA+ESGTSGHASPADQSEIDKLEQHLSNLRKEL+VKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

BLAST of CmoCh10G000500.1 vs. ExPASy TrEMBL
Match: A0A5A7USU3 (Mediator of RNA polymerase II transcription subunit 30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00490 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.9e-91
Identity = 186/198 (93.94%), Postives = 192/198 (96.97%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEK T VL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATT 
Sbjct: 1   MEEKSTTVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASPN PSSHS NGVVNGDASSEST+HHTD+SSGGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNGPSSHSANGVVNGDASSESTSHHTDSSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKAKA+ESGTS HASPADQSEIDKLEQHLSNLRKEL+VKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSAHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

BLAST of CmoCh10G000500.1 vs. ExPASy TrEMBL
Match: A0A1S3CMZ7 (mediator of RNA polymerase II transcription subunit 30 OS=Cucumis melo OX=3656 GN=LOC103502315 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.9e-91
Identity = 186/198 (93.94%), Postives = 192/198 (96.97%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEK T VL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATT 
Sbjct: 1   MEEKSTTVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASPN PSSHS NGVVNGDASSEST+HHTD+SSGGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNGPSSHSANGVVNGDASSESTSHHTDSSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKAKA+ESGTS HASPADQSEIDKLEQHLSNLRKEL+VKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSAHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

BLAST of CmoCh10G000500.1 vs. NCBI nr
Match: XP_022921424.1 (mediator of RNA polymerase II transcription subunit 30 [Cucurbita moschata] >XP_022987135.1 mediator of RNA polymerase II transcription subunit 30 [Cucurbita maxima] >XP_023515464.1 mediator of RNA polymerase II transcription subunit 30 [Cucurbita pepo subsp. pepo] >KAG6589379.1 Mediator of RNA polymerase II transcription subunit 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023058.1 Mediator of RNA polymerase II transcription subunit 30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 367.9 bits (943), Expect = 5.7e-98
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT
Sbjct: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLRELVADVSTWQSPCSV
Sbjct: 181 QLRELVADVSTWQSPCSV 198

BLAST of CmoCh10G000500.1 vs. NCBI nr
Match: XP_004138044.1 (mediator of RNA polymerase II transcription subunit 30 [Cucumis sativus] >KGN63478.1 hypothetical protein Csa_013518 [Cucumis sativus])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 186/198 (93.94%), Postives = 193/198 (97.47%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEK TNVL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATT 
Sbjct: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASP+ PSSHS NGVVNGDASSEST+HHTD+S GGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKAKA+ESGTSGHASPADQSEIDKLEQHLSNLRKEL+VKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

BLAST of CmoCh10G000500.1 vs. NCBI nr
Match: XP_008464424.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 30 [Cucumis melo] >KAA0057767.1 mediator of RNA polymerase II transcription subunit 30 [Cucumis melo var. makuwa] >TYJ98448.1 mediator of RNA polymerase II transcription subunit 30 [Cucumis melo var. makuwa])

HSP 1 Score: 345.1 bits (884), Expect = 3.9e-91
Identity = 186/198 (93.94%), Postives = 192/198 (96.97%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEK T VL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATT 
Sbjct: 1   MEEKSTTVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASPN PSSHS NGVVNGDASSEST+HHTD+SSGGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNGPSSHSANGVVNGDASSESTSHHTDSSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKAKA+ESGTS HASPADQSEIDKLEQHLSNLRKEL+VKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSAHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

BLAST of CmoCh10G000500.1 vs. NCBI nr
Match: XP_038879540.1 (mediator of RNA polymerase II transcription subunit 30 [Benincasa hispida])

HSP 1 Score: 335.5 bits (859), Expect = 3.1e-88
Identity = 183/198 (92.42%), Postives = 190/198 (95.96%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           MEEKPTNVL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSST TT 
Sbjct: 2   MEEKPTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTVTTA 61

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TSGL IASPN PSSHS NGVVNGDASSEST+HHTD+SSGGGSGSGGALEEARVRYKNSVL
Sbjct: 62  TSGLAIASPNGPSSHSVNGVVNGDASSESTSHHTDSSSGGGSGSGGALEEARVRYKNSVL 121

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIPNSQKA A+ESGTSG  SPADQSEIDKLEQHLS+LRKEL+VKNLHIKLLID
Sbjct: 122 ALRAILAAIPNSQKATAYESGTSG--SPADQSEIDKLEQHLSSLRKELVVKNLHIKLLID 181

Query: 181 QLRELVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 182 QLRDLVADVSTWQSPCSV 197

BLAST of CmoCh10G000500.1 vs. NCBI nr
Match: XP_022134927.1 (mediator of RNA polymerase II transcription subunit 30 [Momordica charantia])

HSP 1 Score: 325.1 bits (832), Expect = 4.2e-85
Identity = 174/198 (87.88%), Postives = 187/198 (94.44%), Query Frame = 0

Query: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60
           ME+KPT++L+LPQTTQELA+EGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTA   
Sbjct: 1   MEDKPTSILALPQTTQELAIEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTA--- 60

Query: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120
           TS L+IASPN PS HSTNGVVNGDASSESTNHH DTSSGGG+GSGGALEEARVRY NSVL
Sbjct: 61  TSALSIASPNGPSPHSTNGVVNGDASSESTNHHADTSSGGGTGSGGALEEARVRYNNSVL 120

Query: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180
           ALRAILAAIP+SQ AKA+ESGTSGHASPADQSEIDKLEQHL NLRKEL+ KNLH+KLLID
Sbjct: 121 ALRAILAAIPSSQNAKAYESGTSGHASPADQSEIDKLEQHLCNLRKELVNKNLHLKLLID 180

Query: 181 QLRELVADVSTWQSPCSV 199
           QLR+LVAD+STWQSPCSV
Sbjct: 181 QLRDLVADISTWQSPCSV 195

BLAST of CmoCh10G000500.1 vs. TAIR 10
Match: AT5G63480.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 177.9 bits (450), Expect = 7.8e-45
Identity = 107/190 (56.32%), Postives = 139/190 (73.16%), Query Frame = 0

Query: 14  TTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWST--TSSTATTTTSGLTIASPNA 73
           TTQELAMEG K LEETIE+AFQI+ +MNDELCNP+LWST  T S+A TTT          
Sbjct: 13  TTQELAMEGEKQLEETIEAAFQIISAMNDELCNPSLWSTSATPSSAATTTGS-------- 72

Query: 74  PSSHSTNGVVNGDASS-ESTNHHTDTSSGGGSGSGG--ALEEARVRYKNSVLALRAILAA 133
               + + +V+ DA++ + T+HH++++ GGG G  G   L+EA +RYKNSV +LRA+LAA
Sbjct: 73  ----NGSALVSADAAAIDGTSHHSESAGGGGGGGSGNSVLDEASLRYKNSVTSLRAVLAA 132

Query: 134 IPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLIDQLRELVAD 193
           IPNSQKAKA E   +G  SP  + EI+KLE+   +LR E+  KN+H+K LID+LREL+AD
Sbjct: 133 IPNSQKAKASEM-QNGLGSPESEDEIEKLEEQALSLRMEIAKKNVHVKELIDKLRELIAD 189

Query: 194 VSTWQSPCSV 199
           +STWQSPCSV
Sbjct: 193 ISTWQSPCSV 189

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMV41.1e-4356.32Mediator of RNA polymerase II transcription subunit 30 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1JFZ22.7e-98100.00mediator of RNA polymerase II transcription subunit 30 OS=Cucurbita maxima OX=36... [more]
A0A6J1E0F52.7e-98100.00mediator of RNA polymerase II transcription subunit 30 OS=Cucurbita moschata OX=... [more]
A0A0A0LNW48.6e-9293.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G001500 PE=4 SV=1[more]
A0A5A7USU31.9e-9193.94Mediator of RNA polymerase II transcription subunit 30 OS=Cucumis melo var. maku... [more]
A0A1S3CMZ71.9e-9193.94mediator of RNA polymerase II transcription subunit 30 OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
XP_022921424.15.7e-98100.00mediator of RNA polymerase II transcription subunit 30 [Cucurbita moschata] >XP_... [more]
XP_004138044.11.8e-9193.94mediator of RNA polymerase II transcription subunit 30 [Cucumis sativus] >KGN634... [more]
XP_008464424.13.9e-9193.94PREDICTED: mediator of RNA polymerase II transcription subunit 30 [Cucumis melo]... [more]
XP_038879540.13.1e-8892.42mediator of RNA polymerase II transcription subunit 30 [Benincasa hispida][more]
XP_022134927.14.2e-8587.88mediator of RNA polymerase II transcription subunit 30 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G63480.17.8e-4556.32unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 154..174
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..149
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 54..103
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 54..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 133..152
NoneNo IPR availablePANTHERPTHR36406:SF2MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30coord: 8..198
IPR034568Mediator of RNA polymerase II transcription subunit 30PANTHERPTHR36406MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30coord: 8..198

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh10G000500CmoCh10G000500gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G000500.1:exon:5971CmoCh10G000500.1:exon:5971exon
CmoCh10G000500.1:exon:5970CmoCh10G000500.1:exon:5970exon
CmoCh10G000500.1:exon:5969CmoCh10G000500.1:exon:5969exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G000500.1:three_prime_utrCmoCh10G000500.1:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G000500.1:cdsCmoCh10G000500.1:cds_3CDS
CmoCh10G000500.1:cdsCmoCh10G000500.1:cds_2CDS
CmoCh10G000500.1:cdsCmoCh10G000500.1:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G000500.1:five_prime_utrCmoCh10G000500.1:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh10G000500.1CmoCh10G000500.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016592 mediator complex