Bhi01M000696 (mRNA) Wax gourd (B227) v1

Overview
NameBhi01M000696
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionTranscription termination factor MTEF1
Locationchr1: 17927885 .. 17929523 (-)
Sequence length1639
RNA-Seq ExpressionBhi01M000696
SyntenyBhi01M000696
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACTCCAAAGAGGCTATTTTCGTCAATTACCCTAATTCTCCTATTTTTGCTTCTGATTTCTCCGTCTCCCATTTTTGCCCGCAGCCGCACGACCTCACCGGCCCACTCCGTTTCTTCGTTCTCTCATAGTCTCCATCTCCACTCCTCCGCCGCCCATTTTCCTACTCTGTTCTCTCATAGTTTCATCCTCTTGCGAGGCATTCGTGGTTTCTCCCATTCTTCTTCTCAAGAAGGAAGTAGCAGGTCAGTTTTCCTTCTCCTCGCGTTTTTTTACAGTCATGTATAATCTGCTTCGCAGAATCATTCTAGTTAAACCTCCGTCATCAGTCTTTTCTCACGGATTTTCTAAAAGTCCGTTGAAATCTCTCAGATACTTATCGACCTCTTCTGAGAACGTATCATCTCCGAAACCTGATTCGTTGTCTTATAATTTTGCTCAGCTTAAGAATAATGAAAAGTCTGTAATTATGTTCTTTGAGAATCATGGATTCTCTAAATCACAGATCTCTGAGCTTGTCAAGAAGTTCCCTCAAGTTCTTTCAGCGAACCCCGAGAAAACCCTATTGCCCAAATTGTTGTTCTTTCAATCTAAAGGCCTTTCTTCCCCCGAGATTGCCAAATTAATATGTGCTTTTCCTCGTGTTTTAAAACGAAGTTTAGACAAACAAATTATTCCAGCCTATGATTATATTCAAGCCCTGCTTCGAACTGAGGAGAAGACTGTCGCAACCATAAAACGCTGTATGGGTATTCTTACTTGGAATCTTCGAATTTCAGCTCGTTCTAATATTGAAATTCTAAAACAAATTGGAGTGTCTGATTCCAACATTTCAACCGTTCTTCAGTACCAGCCCAGAATGTTTTTGATAAGTTCCATCAGATTCAAGGAGACTGTAGAGAAAGTTAAGGATATGGGATTCAACCCTCTGCGTTTGAAGTTTGTTCTTGCCGTTTTTGCTCTGCGATCAATGAGCAAATCTACATGGGATAAGAAGATTGAAGTTTATAGGAAATGGGGATGGGGTGAAGAAGAGGTCCGTTTGGCTTTTGGAAGGCATCCATGGTGTATGATGGCTTCTGAGGACAAGATTAATGGTGTAATGGATTATTTTCTTAACAAGATTGGATGTGAATCTTCTTTCATTGCTAGGAGACCTGCTCTAATTTCACTTAGTTTAAAGAACAGGATTGTGCCTAGAGGCTCTATTTATCAAGTTTTGCTGTCAAAGGGTTTGATTAAGAAATATGTGAATCTTCCTTTATTGTTTGAATCTACTGAAAACCGCTTCTTAGAAAAATTCATCAACCCTTATAAGAAGCGGATTCCTGGATTGTTGACCTTGTATAAAGAGAAATTAGAGGATTCTAACAGATGAGCAGGTTCAATCTCCTTGATATGTTAATGCTTACATTTTCTGTTAGTTGACATGTCTATTGACAAAAATTTATTTTCATCAATTTGTGTTTCTACGTTATCTATACTGTTTAGATTGTTACCTAAATGATGATATGATCTTAATACACTAGAGAAACATGCAACTTTTTCATTCAAATACTTCAGTTTCAAAAGAAGGGAAGTTCAACGAGTGGTGTTAGCGTATTTGAACCTCTAATCTTTTGAGTTGAGGTCATATACAC

mRNA sequence

AAACTCCAAAGAGGCTATTTTCGTCAATTACCCTAATTCTCCTATTTTTGCTTCTGATTTCTCCGTCTCCCATTTTTGCCCGCAGCCGCACGACCTCACCGGCCCACTCCGTTTCTTCGTTCTCTCATAGTCTCCATCTCCACTCCTCCGCCGCCCATTTTCCTACTCTGTTCTCTCATAGTTTCATCCTCTTGCGAGGCATTCGTGGTTTCTCCCATTCTTCTTCTCAAGAAGGAAGTAGCAGGTCAGTTTTCCTTCTCCTCGCGTTTTTTTACAGTCATGTATAATCTGCTTCGCAGAATCATTCTAGTTAAACCTCCGTCATCAGTCTTTTCTCACGGATTTTCTAAAAGTCCGTTGAAATCTCTCAGATACTTATCGACCTCTTCTGAGAACGTATCATCTCCGAAACCTGATTCGTTGTCTTATAATTTTGCTCAGCTTAAGAATAATGAAAAGTCTGTAATTATGTTCTTTGAGAATCATGGATTCTCTAAATCACAGATCTCTGAGCTTGTCAAGAAGTTCCCTCAAGTTCTTTCAGCGAACCCCGAGAAAACCCTATTGCCCAAATTGTTGTTCTTTCAATCTAAAGGCCTTTCTTCCCCCGAGATTGCCAAATTAATATGTGCTTTTCCTCGTGTTTTAAAACGAAGTTTAGACAAACAAATTATTCCAGCCTATGATTATATTCAAGCCCTGCTTCGAACTGAGGAGAAGACTGTCGCAACCATAAAACGCTGTATGGGTATTCTTACTTGGAATCTTCGAATTTCAGCTCGTTCTAATATTGAAATTCTAAAACAAATTGGAGTGTCTGATTCCAACATTTCAACCGTTCTTCAGTACCAGCCCAGAATGTTTTTGATAAGTTCCATCAGATTCAAGGAGACTGTAGAGAAAGTTAAGGATATGGGATTCAACCCTCTGCGTTTGAAGTTTGTTCTTGCCGTTTTTGCTCTGCGATCAATGAGCAAATCTACATGGGATAAGAAGATTGAAGTTTATAGGAAATGGGGATGGGGTGAAGAAGAGGTCCGTTTGGCTTTTGGAAGGCATCCATGGTGTATGATGGCTTCTGAGGACAAGATTAATGGTGTAATGGATTATTTTCTTAACAAGATTGGATGTGAATCTTCTTTCATTGCTAGGAGACCTGCTCTAATTTCACTTAGTTTAAAGAACAGGATTGTGCCTAGAGGCTCTATTTATCAAGTTTTGCTGTCAAAGGGTTTGATTAAGAAATATGTGAATCTTCCTTTATTGTTTGAATCTACTGAAAACCGCTTCTTAGAAAAATTCATCAACCCTTATAAGAAGCGGATTCCTGGATTGTTGACCTTGTATAAAGAGAAATTAGAGGATTCTAACAGATGAGCAGGTTCAATCTCCTTGATATGTTAATGCTTACATTTTCTGTTAGTTGACATGTCTATTGACAAAAATTTATTTTCATCAATTTGTGTTTCTACGTTATCTATACTGTTTAGATTGTTACCTAAATGATGATATGATCTTAATACACTAGAGAAACATGCAACTTTTTCATTCAAATACTTCAGTTTCAAAAGAAGGGAAGTTCAACGAGTGGTGTTAGCGTATTTGAACCTCTAATCTTTTGAGTTGAGGTCATATACAC

Coding sequence (CDS)

ATGTATAATCTGCTTCGCAGAATCATTCTAGTTAAACCTCCGTCATCAGTCTTTTCTCACGGATTTTCTAAAAGTCCGTTGAAATCTCTCAGATACTTATCGACCTCTTCTGAGAACGTATCATCTCCGAAACCTGATTCGTTGTCTTATAATTTTGCTCAGCTTAAGAATAATGAAAAGTCTGTAATTATGTTCTTTGAGAATCATGGATTCTCTAAATCACAGATCTCTGAGCTTGTCAAGAAGTTCCCTCAAGTTCTTTCAGCGAACCCCGAGAAAACCCTATTGCCCAAATTGTTGTTCTTTCAATCTAAAGGCCTTTCTTCCCCCGAGATTGCCAAATTAATATGTGCTTTTCCTCGTGTTTTAAAACGAAGTTTAGACAAACAAATTATTCCAGCCTATGATTATATTCAAGCCCTGCTTCGAACTGAGGAGAAGACTGTCGCAACCATAAAACGCTGTATGGGTATTCTTACTTGGAATCTTCGAATTTCAGCTCGTTCTAATATTGAAATTCTAAAACAAATTGGAGTGTCTGATTCCAACATTTCAACCGTTCTTCAGTACCAGCCCAGAATGTTTTTGATAAGTTCCATCAGATTCAAGGAGACTGTAGAGAAAGTTAAGGATATGGGATTCAACCCTCTGCGTTTGAAGTTTGTTCTTGCCGTTTTTGCTCTGCGATCAATGAGCAAATCTACATGGGATAAGAAGATTGAAGTTTATAGGAAATGGGGATGGGGTGAAGAAGAGGTCCGTTTGGCTTTTGGAAGGCATCCATGGTGTATGATGGCTTCTGAGGACAAGATTAATGGTGTAATGGATTATTTTCTTAACAAGATTGGATGTGAATCTTCTTTCATTGCTAGGAGACCTGCTCTAATTTCACTTAGTTTAAAGAACAGGATTGTGCCTAGAGGCTCTATTTATCAAGTTTTGCTGTCAAAGGGTTTGATTAAGAAATATGTGAATCTTCCTTTATTGTTTGAATCTACTGAAAACCGCTTCTTAGAAAAATTCATCAACCCTTATAAGAAGCGGATTCCTGGATTGTTGACCTTGTATAAAGAGAAATTAGAGGATTCTAACAGATGA

Protein sequence

MYNLLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVIMFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVLKRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSNISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVYRKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNRIVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDSNR
Homology
BLAST of Bhi01M000696 vs. TAIR 10
Match: AT5G07900.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 226.9 bits (577), Expect = 2.7e-59
Identity = 117/297 (39.39%), Postives = 185/297 (62.29%), Query Frame = 0

Query: 61  SVIMFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFP 120
           +V+    +HGF+ +QIS LVKK P +L AN E  LLPKL FF S G+S   +A+ + + P
Sbjct: 103 TVLNLLRDHGFTTAQISSLVKKRPVLLLANAESVLLPKLSFFLSIGVSKSLLARTLASDP 162

Query: 121 RVLKRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVS 180
            +L RSL  Q+IP+Y++++++L ++EK VA ++R   +   +   +   NI  + + GV 
Sbjct: 163 TILTRSLVNQLIPSYNFLKSVLDSDEKIVAALRRTTWVFLEDHTKNLVPNINYMAETGVP 222

Query: 181 DSNISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSM-SKSTWDKK 240
           +  I  +L + P   +  +  F+   ++ ++MGFNP +  FVLA+ AL    +KS WDK 
Sbjct: 223 EKCIKLLLTHFPEAVMQKNHEFQAIAKQAQEMGFNPQKSTFVLAIHALSGKGNKSIWDKC 282

Query: 241 IEVYRKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLS 300
            EVY++WGW E+++  AF +HP CMM SE KIN  M+YF+N++      IA+ P ++  S
Sbjct: 283 FEVYQRWGWSEDDIMCAFKKHPHCMMLSERKINRTMEYFVNEMNMAPRSIAQCPVVLFFS 342

Query: 301 LKNRIVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLY 357
           L+ RI+PR S+ +VL+S GL+K+  +L  L    E  FLEK +  Y++ +P L+ LY
Sbjct: 343 LEKRIIPRCSVTKVLVSNGLVKEDWSLTSLLVPVEKVFLEKLVIKYEEELPELMNLY 399

BLAST of Bhi01M000696 vs. TAIR 10
Match: AT1G21150.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.9e-53
Identity = 116/330 (35.15%), Postives = 194/330 (58.79%), Query Frame = 0

Query: 27  LKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVIMFFENHGFSKSQISELVKKFPQV 86
           L+S +  S   + VSS KPD              SV+  F++HGF+  QI+ ++K FP+V
Sbjct: 70  LESAKSNSRFVKLVSSKKPD--------------SVLALFKDHGFTNDQITSVIKSFPRV 129

Query: 87  LSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVLKRSLDKQIIPAYDYIQALLRTEE 146
           LS +PE  + PKL+FF S G S+ + AK+I + P++L  SL K++IP YD ++++L  EE
Sbjct: 130 LSLSPEDVISPKLMFFSSIGFSTSDTAKMISSSPKMLSYSLHKRLIPCYDSLKSILVEEE 189

Query: 147 KTVATIKRCMGILTWNLRIS--ARSNIEILKQIGVSDSNISTVLQYQPRMFLISSIRFKE 206
             V  +KR  GI  ++L+I+      + I +++GV D +I  ++Q  P  F     RF E
Sbjct: 190 SVVKCLKR--GIRCFSLKITHCVSLRVSICRELGVPDKSIKWLVQASPFTFFSRERRFNE 249

Query: 207 TVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVYRKWGWGEEEVRLAFGRHPWCM 266
            + +V   GF+P +  FV A+ A    S+S  ++K ++++ +GW +E+   A  R P C+
Sbjct: 250 VLNRVCSYGFDPKKAGFVHAMVAFDCTSESAMERKFKLFQCFGWSKEDFVAAIMRFPNCV 309

Query: 267 MASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNRIVPRGSIYQVLLSKGLIKKY- 326
             S++KI   ++Y +N IG ++  I  RP ++SLS++ RI PR  +  +LLSKGL+KK  
Sbjct: 310 TVSDEKIMYTLEYLVNNIGLQARDIVARPVVLSLSMEKRIKPRNQVISLLLSKGLVKKED 369

Query: 327 VNLPLLFESTENRFLEKFINPYKKRIPGLL 354
           +N   + +   + F++KF+  Y+  +P L+
Sbjct: 370 INYFTILKLKSSEFMDKFVLKYQDEMPQLV 383

BLAST of Bhi01M000696 vs. TAIR 10
Match: AT5G64950.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 157.5 bits (397), Expect = 2.0e-38
Identity = 96/324 (29.63%), Postives = 180/324 (55.56%), Query Frame = 0

Query: 43  PKPDSLSYNFAQLKNNE--KSVIMFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLL 102
           P   +++  +  LK+ E  +SVI   +++ FS +QI + ++  P+++  N EK L PKL 
Sbjct: 53  PLAIAIAMRYPNLKSLEQPRSVIQMLKSYSFSDTQIQKSIRVHPRMMFYNVEKILEPKLR 112

Query: 103 FFQSKGLSSPEIAKLICAFPRVLKRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMG--I 162
           FF+  G +   + K +     V+  SL K++IP  + +++++  + + +  I    G  +
Sbjct: 113 FFKDIGFTGSGLGKFVSQNSSVVGVSLVKKLIPTVEILKSIVAPKHEDLPVILSRCGWLL 172

Query: 163 LTWNLRISARSNIEILKQIGVSDSNISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLR 222
           L+ +  +    NI  L+  G+  S ++++L+ QPR+F +S  + +  V +  D+GF    
Sbjct: 173 LSRDPNLFLLPNISYLETCGIVGSQLASLLRRQPRIFNLSEEKLRGYVSRALDLGFTLNS 232

Query: 223 LKFVLAVFALRSMSKSTWDKKIEVYRKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYF 282
              V AV +L S+S+ T+D+K++++   G+ E+E+     R P  +  SEDK+    +++
Sbjct: 233 RMLVHAVISLSSLSEKTFDRKVKLFMANGFSEDEITDIIRRSPGLIRCSEDKLTLGFEFY 292

Query: 283 LNKIGCESSFIARRPALISLSLKNRIVPRGSIYQVLLSKGLI----KKYVNLPLLFESTE 342
           L ++G E   +A+RP ++S +L+ R++PR  + Q+L  KGL+    KK  N+  + E TE
Sbjct: 293 LKRMGLEREALAKRPCVLSYNLEKRVIPRLKVLQILREKGLLLKEEKKKKNMVGIVEMTE 352

Query: 343 NRFLEKFINPYKKRI-PGLLTLYK 358
             FLEK++  +   I   LL  YK
Sbjct: 353 EAFLEKYVVRFGDEIAEELLVAYK 376

BLAST of Bhi01M000696 vs. TAIR 10
Match: AT1G61970.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 145.2 bits (365), Expect = 1.0e-34
Identity = 99/342 (28.95%), Postives = 177/342 (51.75%), Query Frame = 0

Query: 56  KNNEKSVIMFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL 115
           KNN  SV+    +HGF+ SQIS +++ +PQ+L A+ EK+L PKL F QS+G SS EI ++
Sbjct: 81  KNNPDSVLNLLTSHGFTGSQISTIIRDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEI 140

Query: 116 ICAFPRVLKRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILK 175
           + + P +L +   K I   YD+I+  L   EK+    K C  +   NL    R N+ +L+
Sbjct: 141 VSSVPEILGKKGHKTISVYYDFIKDTLL--EKSSKNEKLCHSLPQGNLENKIR-NVSVLR 200

Query: 176 QIGVSDSNISTVL--QYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSK 235
           ++G+    + ++L    QP   +    +F+ET++KV +MGF+P   KFV A+  +  M++
Sbjct: 201 ELGMPHKLLFSLLISDSQP---VCGKEKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNE 260

Query: 236 STWDKKIEVYRKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFL-------------- 295
            T ++K+ +Y+  G+   +V  +F + P  +  SE K+   ++ FL              
Sbjct: 261 KTIEEKVHLYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVK 320

Query: 296 ---------------------NKIGCESSFIARRPALISLSLKNRIVPRGSIYQVLLSKG 355
                                 K+      +   PA+   SL+ RIVPRG++ + L+SKG
Sbjct: 321 HFPPCIGLSTETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKG 380

Query: 356 LIKKYV-NLPLLFESTENRFLEKFINPY--KKRIPGLLTLYK 358
           L++  + ++  +   T+  FL +++  +  K+ +  L+ +Y+
Sbjct: 381 LMRNELPSISCVLMCTKQVFLNRYVANHVDKQLVTELMAIYR 416

BLAST of Bhi01M000696 vs. TAIR 10
Match: AT1G61970.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 145.2 bits (365), Expect = 1.0e-34
Identity = 99/342 (28.95%), Postives = 177/342 (51.75%), Query Frame = 0

Query: 56  KNNEKSVIMFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL 115
           KNN  SV+    +HGF+ SQIS +++ +PQ+L A+ EK+L PKL F QS+G SS EI ++
Sbjct: 81  KNNPDSVLNLLTSHGFTGSQISTIIRDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEI 140

Query: 116 ICAFPRVLKRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILK 175
           + + P +L +   K I   YD+I+  L   EK+    K C  +   NL    R N+ +L+
Sbjct: 141 VSSVPEILGKKGHKTISVYYDFIKDTLL--EKSSKNEKLCHSLPQGNLENKIR-NVSVLR 200

Query: 176 QIGVSDSNISTVL--QYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSK 235
           ++G+    + ++L    QP   +    +F+ET++KV +MGF+P   KFV A+  +  M++
Sbjct: 201 ELGMPHKLLFSLLISDSQP---VCGKEKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNE 260

Query: 236 STWDKKIEVYRKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFL-------------- 295
            T ++K+ +Y+  G+   +V  +F + P  +  SE K+   ++ FL              
Sbjct: 261 KTIEEKVHLYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVK 320

Query: 296 ---------------------NKIGCESSFIARRPALISLSLKNRIVPRGSIYQVLLSKG 355
                                 K+      +   PA+   SL+ RIVPRG++ + L+SKG
Sbjct: 321 HFPPCIGLSTETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKG 380

Query: 356 LIKKYV-NLPLLFESTENRFLEKFINPY--KKRIPGLLTLYK 358
           L++  + ++  +   T+  FL +++  +  K+ +  L+ +Y+
Sbjct: 381 LMRNELPSISCVLMCTKQVFLNRYVANHVDKQLVTELMAIYR 416

BLAST of Bhi01M000696 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.0e-07
Identity = 49/261 (18.77%), Postives = 116/261 (44.44%), Query Frame = 0

Query: 62  VIMFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPR 121
           V+ + +      S +  +++++P+VL    E T+   + +    G++  EI  ++  +P 
Sbjct: 218 VVKYLQGLDIKPSDVPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPE 277

Query: 122 VLKRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSD 181
           +L   + + I P  +Y++ L          I++   IL + L  + + N++IL+   V +
Sbjct: 278 ILGMRVARIIKPLVEYLEVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRE 337

Query: 182 SNISTVLQYQPRMFLISSIRFKETVEKV--KDMGFNPLRL-KFVLAVFALRSMSKSTWDK 241
           +++ +++   P +  I      +T  K+    +  NP  L   +  +    S+S+S   K
Sbjct: 338 TSLPSIIAQYPEIIGIDLKPKLDTQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLK 397

Query: 242 KIEVYRKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISL 301
            I+   K G+  ++ R      P  +  +   +    +YF  ++      +   PA  + 
Sbjct: 398 HIDFLTKCGFSIDQTREMVIGCPQVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTY 457

Query: 302 SLKNRIVPRGSIYQVLLSKGL 320
            L++ + PR   ++ ++ KG+
Sbjct: 458 GLESTVKPR---HKKIIKKGI 475

BLAST of Bhi01M000696 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 7.5e-06
Identity = 41/191 (21.47%), Postives = 96/191 (50.26%), Query Frame = 0

Query: 70  GFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVLKRSLDK 129
           G +  +++  + KFP +LS + E+ L P L FFQ+ G+   ++ K+I   PR++  S+D 
Sbjct: 89  GRNPREVASAITKFPPILSHSVEEKLCPLLAFFQALGVPETQLGKMILFNPRLISYSIDT 148

Query: 130 QIIPAYDYIQALLRTEEKTVA-TIKRCMGILTWNLRISARSNIEILK-QIGVSDSNISTV 189
           ++     ++ +L   ++  +   + +   ++ +++    R   E LK  +G+S+  I +V
Sbjct: 149 KLTVIVSFLASLGLDQDGMIGKVLVKNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSV 208

Query: 190 LQYQPRMFL--ISSIRFKETVEKVKDMGFNPLRLKFVLAVF---ALRSMSKSTWDKKIEV 249
           +   P++    ++ I  K   + +K+ GF   ++  ++  +    ++S+  S   +   +
Sbjct: 209 VMNFPQLLCRDVNKI-LKPNYDYLKECGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFL 268

Query: 250 YRKWGWGEEEV 254
            +  G G +EV
Sbjct: 269 VQVMGRGMDEV 278

BLAST of Bhi01M000696 vs. ExPASy Swiss-Prot
Match: B6TGN4 (Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MTERF4 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 3.7e-05
Identity = 51/286 (17.83%), Postives = 121/286 (42.31%), Query Frame = 0

Query: 62  VIMFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPR 121
           V+ + +      + +  +++++P++L    E T+   + +    G+   ++  +I  FP 
Sbjct: 174 VVKYLQGMDVRPTDVPRVLERYPELLGFKLEGTMSTSVAYLVGIGVGRRQVGSVITRFPE 233

Query: 122 VLKRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSD 181
           VL   + K I P  ++++ +          I++   +L + L+   + NIE L  IGV  
Sbjct: 234 VLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLQEKVKPNIEALVDIGVRK 293

Query: 182 SNISTVLQYQP-------RMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKS 241
             +++++   P       R  L++     E+   V    F  +  +   A+    S+ ++
Sbjct: 294 EALASIVMQYPDVLGLELRDKLVAQQSLFESSILVSREDFGRVLERMPQAI----SLGRA 353

Query: 242 TWDKKIEVYRKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPA 301
              K +      G+   +V       P  +  + D +    +YF N++  +   +   PA
Sbjct: 354 AVLKHVNFLTACGFMLSQVSKMVVACPQLLALNIDIMRMNFEYFKNEMERDLEELVEFPA 413

Query: 302 LISLSLKNRIVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEK 341
             +  +++ + PR   ++++  KGL     +L  L   ++ +F E+
Sbjct: 414 FFTYGIESTVRPR---HEMVSRKGLT---CSLAWLLNCSDAKFDER 449

BLAST of Bhi01M000696 vs. ExPASy Swiss-Prot
Match: Q84X53 (Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF1 PE=2 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 6.3e-05
Identity = 36/140 (25.71%), Postives = 70/140 (50.00%), Query Frame = 0

Query: 61  SVIMFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSK-GLSSPEIAKLICAF 120
           SV     + G S+  +  ++  FP +L+++PE  +LP L F  ++  +S  +I K I   
Sbjct: 67  SVETLLSSTGLSRPAVGRILDMFPDLLTSDPESEILPVLRFLSNEISISEQDIPKSISRC 126

Query: 121 PRVLKRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQ-IG 180
           PR+L  S+D Q+ PA  +++ L      T+ +  R   +L  N+  +    IE L++ +G
Sbjct: 127 PRLLISSVDYQLRPALTFLKTLGFVGRDTITS--RNTVLLVSNVERTLIPKIEYLEEGLG 186

Query: 181 VSDSNISTVLQYQPRMFLIS 199
            +   ++ ++   P +   S
Sbjct: 187 FTREEVAKMVVRSPALLTYS 204

BLAST of Bhi01M000696 vs. NCBI nr
Match: XP_022933339.1 (uncharacterized protein LOC111440613 isoform X2 [Cucurbita moschata])

HSP 1 Score: 558.9 bits (1439), Expect = 3.2e-155
Identity = 278/362 (76.80%), Postives = 323/362 (89.23%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRI +V+ PSSVF+HGFS+SPLKSLRYLSTSSE  SSPK  S   N  + +N+EK VI
Sbjct: 97  LLRRINVVRQPSSVFAHGFSESPLKSLRYLSTSSEIASSPKSASPDSNKVKPENDEKPVI 156

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           +FFENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 157 VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 216

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQ LL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 217 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 276

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+L+YQPR+FL++++RFKE VE+VK+MGFNPLRL FVLAVFA+R+MSKSTW KK+EVY
Sbjct: 277 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 336

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMMASEDKINGVMD+F+NKIGCE S+IA+RPALISLSLK R
Sbjct: 337 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 396

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL+KFI+P+K+ IPGLL LYKEKLEDS
Sbjct: 397 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 456

Query: 364 NR 366
            R
Sbjct: 457 KR 458

BLAST of Bhi01M000696 vs. NCBI nr
Match: XP_022933338.1 (uncharacterized protein LOC111440613 isoform X1 [Cucurbita moschata])

HSP 1 Score: 558.9 bits (1439), Expect = 3.2e-155
Identity = 278/362 (76.80%), Postives = 323/362 (89.23%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRI +V+ PSSVF+HGFS+SPLKSLRYLSTSSE  SSPK  S   N  + +N+EK VI
Sbjct: 98  LLRRINVVRQPSSVFAHGFSESPLKSLRYLSTSSEIASSPKSASPDSNKVKPENDEKPVI 157

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           +FFENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 158 VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 217

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQ LL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 218 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 277

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+L+YQPR+FL++++RFKE VE+VK+MGFNPLRL FVLAVFA+R+MSKSTW KK+EVY
Sbjct: 278 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 337

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMMASEDKINGVMD+F+NKIGCE S+IA+RPALISLSLK R
Sbjct: 338 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 397

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL+KFI+P+K+ IPGLL LYKEKLEDS
Sbjct: 398 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 457

Query: 364 NR 366
            R
Sbjct: 458 KR 459

BLAST of Bhi01M000696 vs. NCBI nr
Match: XP_022956116.1 (uncharacterized protein LOC111457902 isoform X2 [Cucurbita moschata])

HSP 1 Score: 555.4 bits (1430), Expect = 3.5e-154
Identity = 277/362 (76.52%), Postives = 320/362 (88.40%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRII+V  PSSVF HGFS+SPLKSLRY STSSE  SSPK  S   N  + +N+EK VI
Sbjct: 3   LLRRIIVVSRPSSVFVHGFSESPLKSLRYSSTSSEIASSPKSASPDSNSVEPENDEKPVI 62

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           + FENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 63  VLFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQ LL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 123 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+L+YQPR+FL++++RFKE VE+VK+MGFNPLRL FVLAVFA+R+MSKSTW KK+EVY
Sbjct: 183 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMMASEDKINGVMD+F+NKIGCE S+IA+RPALISLSLK R
Sbjct: 243 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 302

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL+KFI+P+K+ IPGLL LYKEKLEDS
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 362

Query: 364 NR 366
            R
Sbjct: 363 KR 364

BLAST of Bhi01M000696 vs. NCBI nr
Match: XP_022979833.1 (uncharacterized protein LOC111479414 [Cucurbita maxima])

HSP 1 Score: 553.1 bits (1424), Expect = 1.8e-153
Identity = 276/362 (76.24%), Postives = 320/362 (88.40%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRII+V+ PSSVF+HGFS+SP KSLRY STSS   SS K  S   N  + +NNEK VI
Sbjct: 3   LLRRIIVVRQPSSVFAHGFSESPFKSLRYSSTSSGIASSLKSASPDSNSVKPENNEKPVI 62

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           +FFENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 63  VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQALL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 123 TRSLDRKIIPAFDYIQALLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+LQYQPR+FLI+++RFKE VE+VK+MGFNPLRL FVLAVFA+R+MSKSTW KK+EVY
Sbjct: 183 IATILQYQPRVFLINNVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMMASEDKINGVMD+F+NK+GCE S+IA+RPALISLSLK R
Sbjct: 243 KKWGWPEEEILFAFRRHPWCMMASEDKINGVMDFFVNKLGCEPSYIAKRPALISLSLKKR 302

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL+KFI+P+KK IPGLL LYK+KL D+
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKKLIPGLLKLYKKKLVDA 362

Query: 364 NR 366
            R
Sbjct: 363 KR 364

BLAST of Bhi01M000696 vs. NCBI nr
Match: XP_023527658.1 (transcription termination factor MTERF4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548.1 bits (1411), Expect = 5.6e-152
Identity = 272/362 (75.14%), Postives = 320/362 (88.40%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRI +V+ PSSVF+H FS+SPLKSLRY STSSE  SSPK  S + N  + +N+EK VI
Sbjct: 3   LLRRINVVRQPSSVFAHEFSESPLKSLRYSSTSSEIASSPKSASPNSNSVKPENDEKPVI 62

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           +FFENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C+FP +L
Sbjct: 63  VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCSFPSIL 122

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQALL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 123 MRSLDRKIIPAFDYIQALLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+L+YQPR+FL++++RFKE VE+VK+MGFNPLRL FV+AV A+R+MSKSTW KK+EVY
Sbjct: 183 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVIAVLAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMM SEDKINGVMD+F+NKIG + S+IA+RPALISLSLK R
Sbjct: 243 KKWGWSEEEILFAFRRHPWCMMVSEDKINGVMDFFVNKIGSDPSYIAKRPALISLSLKKR 302

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL KFI+P+KK IPGLL LYKEKLED+
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLNKFIDPHKKLIPGLLKLYKEKLEDA 362

Query: 364 NR 366
            R
Sbjct: 363 KR 364

BLAST of Bhi01M000696 vs. ExPASy TrEMBL
Match: A0A6J1EZH0 (uncharacterized protein LOC111440613 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111440613 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.5e-155
Identity = 278/362 (76.80%), Postives = 323/362 (89.23%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRI +V+ PSSVF+HGFS+SPLKSLRYLSTSSE  SSPK  S   N  + +N+EK VI
Sbjct: 97  LLRRINVVRQPSSVFAHGFSESPLKSLRYLSTSSEIASSPKSASPDSNKVKPENDEKPVI 156

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           +FFENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 157 VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 216

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQ LL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 217 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 276

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+L+YQPR+FL++++RFKE VE+VK+MGFNPLRL FVLAVFA+R+MSKSTW KK+EVY
Sbjct: 277 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 336

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMMASEDKINGVMD+F+NKIGCE S+IA+RPALISLSLK R
Sbjct: 337 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 396

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL+KFI+P+K+ IPGLL LYKEKLEDS
Sbjct: 397 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 456

Query: 364 NR 366
            R
Sbjct: 457 KR 458

BLAST of Bhi01M000696 vs. ExPASy TrEMBL
Match: A0A6J1F4G8 (uncharacterized protein LOC111440613 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440613 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.5e-155
Identity = 278/362 (76.80%), Postives = 323/362 (89.23%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRI +V+ PSSVF+HGFS+SPLKSLRYLSTSSE  SSPK  S   N  + +N+EK VI
Sbjct: 98  LLRRINVVRQPSSVFAHGFSESPLKSLRYLSTSSEIASSPKSASPDSNKVKPENDEKPVI 157

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           +FFENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 158 VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 217

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQ LL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 218 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 277

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+L+YQPR+FL++++RFKE VE+VK+MGFNPLRL FVLAVFA+R+MSKSTW KK+EVY
Sbjct: 278 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 337

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMMASEDKINGVMD+F+NKIGCE S+IA+RPALISLSLK R
Sbjct: 338 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 397

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL+KFI+P+K+ IPGLL LYKEKLEDS
Sbjct: 398 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 457

Query: 364 NR 366
            R
Sbjct: 458 KR 459

BLAST of Bhi01M000696 vs. ExPASy TrEMBL
Match: A0A6J1GVP2 (uncharacterized protein LOC111457902 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457902 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.7e-154
Identity = 277/362 (76.52%), Postives = 320/362 (88.40%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRII+V  PSSVF HGFS+SPLKSLRY STSSE  SSPK  S   N  + +N+EK VI
Sbjct: 3   LLRRIIVVSRPSSVFVHGFSESPLKSLRYSSTSSEIASSPKSASPDSNSVEPENDEKPVI 62

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           + FENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 63  VLFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQ LL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 123 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+L+YQPR+FL++++RFKE VE+VK+MGFNPLRL FVLAVFA+R+MSKSTW KK+EVY
Sbjct: 183 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMMASEDKINGVMD+F+NKIGCE S+IA+RPALISLSLK R
Sbjct: 243 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 302

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL+KFI+P+K+ IPGLL LYKEKLEDS
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 362

Query: 364 NR 366
            R
Sbjct: 363 KR 364

BLAST of Bhi01M000696 vs. ExPASy TrEMBL
Match: A0A6J1IXF7 (uncharacterized protein LOC111479414 OS=Cucurbita maxima OX=3661 GN=LOC111479414 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 8.5e-154
Identity = 276/362 (76.24%), Postives = 320/362 (88.40%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRII+V+ PSSVF+HGFS+SP KSLRY STSS   SS K  S   N  + +NNEK VI
Sbjct: 3   LLRRIIVVRQPSSVFAHGFSESPFKSLRYSSTSSGIASSLKSASPDSNSVKPENNEKPVI 62

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           +FFENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 63  VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQALL+TEEKT+A+IKR +GILTW+L+I AR N+E L QIGV DS 
Sbjct: 123 TRSLDRKIIPAFDYIQALLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+LQYQPR+FLI+++RFKE VE+VK+MGFNPLRL FVLAVFA+R+MSKSTW KK+EVY
Sbjct: 183 IATILQYQPRVFLINNVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+  AF RHPWCMMASEDKINGVMD+F+NK+GCE S+IA+RPALISLSLK R
Sbjct: 243 KKWGWPEEEILFAFRRHPWCMMASEDKINGVMDFFVNKLGCEPSYIAKRPALISLSLKKR 302

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRGS+YQVLLSKGLIKK VNLP LFES ENRFL+KFI+P+KK IPGLL LYK+KL D+
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKKLIPGLLKLYKKKLVDA 362

Query: 364 NR 366
            R
Sbjct: 363 KR 364

BLAST of Bhi01M000696 vs. ExPASy TrEMBL
Match: A0A6J1EK58 (uncharacterized protein LOC111434072 OS=Cucurbita moschata OX=3662 GN=LOC111434072 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 8.0e-152
Identity = 268/362 (74.03%), Postives = 323/362 (89.23%), Query Frame = 0

Query: 4   LLRRIILVKPPSSVFSHGFSKSPLKSLRYLSTSSENVSSPKPDSLSYNFAQLKNNEKSVI 63
           LLRRII+V+ PSSVF+HGFS+SPLKSLRY STSSE  SSPK  S   N  + +N+EK VI
Sbjct: 3   LLRRIIIVRQPSSVFAHGFSESPLKSLRYSSTSSEIASSPKSASPDSNSVRPENDEKPVI 62

Query: 64  MFFENHGFSKSQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLICAFPRVL 123
           + FENHGFSK+QISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKL+C FP +L
Sbjct: 63  VLFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLDKQIIPAYDYIQALLRTEEKTVATIKRCMGILTWNLRISARSNIEILKQIGVSDSN 183
            RSLD++IIPA+DYIQ LL+T+EK +A+IKR +GILTW+L+ + R N+E L QIGV DSN
Sbjct: 123 TRSLDRKIIPAFDYIQVLLQTKEKAIASIKRSLGILTWDLQ-TVRPNVETLIQIGVPDSN 182

Query: 184 ISTVLQYQPRMFLISSIRFKETVEKVKDMGFNPLRLKFVLAVFALRSMSKSTWDKKIEVY 243
           I+T+LQYQPR+FL++++RFKE VE VK+MGFNPLRLKFVLAVFA+R+MSK TW KK+EVY
Sbjct: 183 IATILQYQPRVFLVNTVRFKEIVEDVKEMGFNPLRLKFVLAVFAMRAMSKCTWRKKVEVY 242

Query: 244 RKWGWGEEEVRLAFGRHPWCMMASEDKINGVMDYFLNKIGCESSFIARRPALISLSLKNR 303
           +KWGW EEE+ +AF RHPWCMMASEDKING+MD+F+NKIGCE+S++A+RPA+I+LSLK R
Sbjct: 243 KKWGWPEEEILVAFRRHPWCMMASEDKINGIMDFFVNKIGCETSYVAKRPAVITLSLKKR 302

Query: 304 IVPRGSIYQVLLSKGLIKKYVNLPLLFESTENRFLEKFINPYKKRIPGLLTLYKEKLEDS 363
           IVPRG +YQVLLSKGLIKK VNLP++FESTE+RFL+KFI+P+K+ IPGLL LYKEKLED+
Sbjct: 303 IVPRGFVYQVLLSKGLIKKDVNLPMVFESTESRFLDKFIDPHKELIPGLLKLYKEKLEDA 362

Query: 364 NR 366
            R
Sbjct: 363 KR 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G07900.12.7e-5939.39Mitochondrial transcription termination factor family protein [more]
AT1G21150.12.9e-5335.15Mitochondrial transcription termination factor family protein [more]
AT5G64950.12.0e-3829.63Mitochondrial transcription termination factor family protein [more]
AT1G61970.11.0e-3428.95Mitochondrial transcription termination factor family protein [more]
AT1G61970.21.0e-3428.95Mitochondrial transcription termination factor family protein [more]
Match NameE-valueIdentityDescription
Q9ZT963.0e-0718.77Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL67.5e-0621.47Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
B6TGN43.7e-0517.83Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MT... [more]
Q84X536.3e-0525.71Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_022933339.13.2e-15576.80uncharacterized protein LOC111440613 isoform X2 [Cucurbita moschata][more]
XP_022933338.13.2e-15576.80uncharacterized protein LOC111440613 isoform X1 [Cucurbita moschata][more]
XP_022956116.13.5e-15476.52uncharacterized protein LOC111457902 isoform X2 [Cucurbita moschata][more]
XP_022979833.11.8e-15376.24uncharacterized protein LOC111479414 [Cucurbita maxima][more]
XP_023527658.15.6e-15275.14transcription termination factor MTERF4, chloroplastic-like [Cucurbita pepo subs... [more]
Match NameE-valueIdentityDescription
A0A6J1EZH01.5e-15576.80uncharacterized protein LOC111440613 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1F4G81.5e-15576.80uncharacterized protein LOC111440613 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1GVP21.7e-15476.52uncharacterized protein LOC111457902 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IXF78.5e-15476.24uncharacterized protein LOC111479414 OS=Cucurbita maxima OX=3661 GN=LOC111479414... [more]
A0A6J1EK588.0e-15274.03uncharacterized protein LOC111434072 OS=Cucurbita moschata OX=3662 GN=LOC1114340... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 77..108
e-value: 1.9E-5
score: 34.1
coord: 286..320
e-value: 11.0
score: 15.0
coord: 185..215
e-value: 140.0
score: 10.6
coord: 254..285
e-value: 1.8
score: 17.5
coord: 113..144
e-value: 0.18
score: 20.9
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 64..217
e-value: 4.7E-23
score: 81.6
coord: 169..337
e-value: 8.5E-18
score: 64.3
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 58..362
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 144..349
e-value: 4.5E-43
score: 149.3
NoneNo IPR availablePANTHERPTHR13068:SF160TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 58..362

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi01G000696Bhi01G000696gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi01M000696.exon1Bhi01M000696.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi01M000696.utr3p1Bhi01M000696.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi01M000696cds.Bhi01M000696CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi01M000696.utr5p1Bhi01M000696.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi01M000696Bhi01M000696-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0006353 DNA-templated transcription, termination
molecular_function GO:0003690 double-stranded DNA binding